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• The metamafic rocks can be classified into high-Fe and low-Fe groups, and the former is similar to N-MORB and the latter is similar to E-MORB. • The high-Fe and low-Fe groups may have been derived from different parts of a mantle plume. • The Tarim Craton, North China Craton, and North Australia Craton may potentially dominated by the same superplume. In the Mesoproterozoic, there was an epic transition on Earth, the final breakup of the Columbia supercontinent. Understanding the dynamic mechanism responsible for the final breakup of Columbia supercontinent is crucial for establishing Earth's evolution that includes geology, environment and life. However, there is little research on what drove the breakup of the Columbia supercontinent. Here we focus on this issue by integrating new zircon U-Pb ages, Lu-Hf isotopes, and whole-rock geochemical data for metamafic rocks from the Oulongbuluke Block in the southeast of Tarim Craton. These data show that the protoliths of these metamafic rocks were emplaced at ca. 1.37–1.35 Ga and were divided into high-Fe and low-Fe groups. The high-Fe and low-Fe groups show geochemical character similar to normal mid-ocean ridge basalt (N-MORB) and enriched mid-ocean ridge basalt (E-MORB), respectively. The high-Fe group was formed by high degree (7%-9%) partial melting of spinel-phase lherzolite mantle source, whereas the low-Fe group was derived from low degree (5%-6%) partial melting of spinel-phase lherzolite mantle source. We propose that the high-Fe and low-Fe groups may have been derived from magmas from different parts of a mantle plume. The formation of the 1.37–1.35 Ga metamafic rocks in the Oulongbuluke Block may be related to the separation of the Tarim Craton into North and South Tarim cratons, which resulted in the opening of the initial Middle Tarim Ocean Basin. By comparison with the plume-induced Yanliao Rift of North China Craton and McArthur Basin of North Australia Craton, we present that these three cratons may potentially dominated by a superplume, forming circular rift zone and radiating dykes. This superplume triggered the continuous extension to most parts of Columbia and led to the final breakup of the Columbia supercontinent. [ABSTRACT FROM AUTHOR]