1. Magnetic-responsive upconversion luminescence resonance energy transfer (LRET) biosensor for ultrasensitive detection of SARS-CoV-2 spike protein.
- Author
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Chen, Jiareng, Ho, Willis Kwun Hei, Yin, Bohan, Zhang, Qin, Li, Chuanqi, Yan, Jiaxiang, Huang, Yingying, Hao, Jianhua, Yi, Changqing, Zhang, Yu, Wong, Siu Hong Dexter, and Yang, Mo
- Subjects
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FLUORESCENCE resonance energy transfer , *PHOTON upconversion , *LUMINESCENCE , *BIOSENSORS , *SARS-CoV-2 - Abstract
Upconversion nanoparticles (UCNPs) are ideal donors for luminescence resonance energy transfer (LRET)-based biosensors due to their excellent upconversion luminescence properties. However, the relatively large size of antibodies and proteins limits the application of UCNPs-based LRET biosensors in protein detection because the large steric hindrance of proteins leads to low energy transfer efficiency between UCNPs and receptors. Herein, we developed a magnetic responsive UCNPs-based LRET biosensor to control the coupling distance between antibody-functionalized UCNPs (Ab-UCNPs) as donors and antibody-PEG linker-magnetic gold nanoparticles (Ab-PEG-MGNs) as acceptors for ultrasensitive and highly selective detection of SARS-CoV-2 spike proteins. Our results showed that this platform reversibly shortened the coupling distance between UCNPs and MGNs and enhanced the LRET signal with a 10-fold increase in the limit of detection (LOD) from 20.6 pg/mL without magnetic modulation to 2.1 pg/mL with magnetic modulation within 1 h. The finite-difference time-domain (FDTD) simulation with cyclic distance change confirmed the distance-dependent LRET efficiency under magnetic modulation, which supported the experimental results. Moreover, the applications of this magnetic-responsive UCNP-based LRET biosensor could be extended to other large-size biomolecule detection. • Development of a magnetic-responsive upconversion luminescence resonance energy transfer (LRET) protein biosensor. • Magnetic modulation could overcome the space hindrance of protein to enhance energy tranfer efficiency. • A low limit of detection down to 2.1 pg/mL under magnetic modulation was achieved. • A rapid detection time within 40 min was achieved. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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