• Introducing climate (CS) and vegetation seasonality (VS) into the Budyko framework. • CS and VS jointly contribute more than the changes in P and ET 0 in water partitioning. • In average the contributions of VS and CS in water partitioning are equally important. Climate and vegetation changes are main factors influencing water partitioning. However, their impacts on water partitioning are still not well understood. To revisit the interactions among climate, vegetation and water partitioning, this study introduces the climate seasonality (CS) and vegetation seasonality (VS) into the Budyko framework, by using a statistical approach combining the three-parameter Log-logistic probability distribution and multiple linear regression, based on the monthly 〈 P - ET 0 〉 (precipitation (P) minus potential evapotranspiration ( ET 0)) and Normalized Difference Vegetation Index (NDVI). This method is then validated and applied in 86 global catchments to quantify the contribution of climate and vegetation changes to evapotranspiration (ET). Results show that this model can predict well (mean R 2 = 0.81 ± 0.05, P < 0.05) the parameter n , a parameter reflecting the interactions between land surface and atmosphere in the Budyko framework. More importantly, CS and VS jointly control the variation in parameter n , and in average jointly contribute more (mean relative contribution of 57.1 ± 2.3 %) than the changes in P and ET 0 (mean of 42.9 ± 2.3%) to ET. These indicate that CS and VS are at least as important as the changes in P and ET 0 in affecting water balance. Furthermore, the VS contributes slightly lower (mean of 24.9 ± 0.3%) than the CS does (mean of 32.2 ± 2.3%), implying that CS and VS play comparable roles in water partitioning. These findings would be valuable for water resources management and assessing the impacts of climate and land surface changes on water balance in a gradually changing environment. [ABSTRACT FROM AUTHOR]