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Equine odontoclastic tooth resorption and hypercementosis (EOTRH) is one of the horses' dental diseases, mainly affecting the incisor teeth. An increase in the incidence of aged horses and a painful progressive course of the disease create the need for improved early diagnosis. Besides clinical findings, EOTRH recognition is based on the typical radiographic findings, including levels of dental resorption and hypercementosis. This study aimed to introduce digital processing methods to equine dental radiographic images and identify texture features changing with disease progression. The radiographs of maxillary incisor teeth from 80 horses were obtained. Each incisor was annotated by separate masks and clinically classified as 0, 1, 2, or 3 EOTRH degrees. Images were filtered by Mean, Median, Normalize, Bilateral, Binomial, CurvatureFlow, LaplacianSharpening, DiscreteGaussian, and SmoothingRecursiveGaussian filters independently, and 93 features of image texture were extracted using First Order Statistics (FOS), Gray Level Co-occurrence Matrix (GLCM), Neighbouring Gray Tone Difference Matrix (NGTDM), Gray Level Dependence Matrix (GLDM), Gray Level Run Length Matrix (GLRLM), and Gray Level Size Zone Matrix (GLSZM) approaches. The most informative processing was selected. GLCM and GLRLM return the most favorable features for the quantitative evaluation of radiographic signs of the EOTRH syndrome, which may be supported by filtering by filters improving the edge delimitation. [ABSTRACT FROM AUTHOR]

Simple Summary: Detecting horse state after exercise is critical for maximizing athletic performance. The horse's response to fatigue includes exercise termination or exercise continuation at a lower intensity, which significantly limit the results achieved in races and equestrian competition. As conventional methods of detecting and quantifying exercise effort have shown some limitations, infrared thermography was proposed as a method of contactless detection of exercise effect. The promising correlation between body surface temperature and exercise-dependent blood biomarkers has been demonstrated. As the application of conventional thermography is limited by low specificity, advanced thermal image analysis was proposed here to visualize the link between blood biomarkers and texture of thermal images. Twelve horses underwent standardized exercise tests for six consecutive days, and both thermal images and blood samples were collected before and after each test. The images were analyzed using four color models (RGB, red-green-blue; YUV, brightness-UV-components; YIQ, brightness-IQ-components; HSB, hue-saturation-brightness) and eight texture-features approaches, including 88 features in total. In contrast to conventional temperature measures, as many as twelve texture features in two color models (RGB, YIQ) were linked with blood biomarker levels as part of the horse's response to exercise. As the detection of horse state after exercise is constantly developing, a link between blood biomarkers and infrared thermography (IRT) was investigated using advanced image texture analysis. The aim of the study was to determine which combinations of RGB (red-green-blue), YUI (brightness-UV-components), YIQ (brightness-IQ-components), and HSB (hue-saturation-brightness) color models, components, and texture features are related to the blood biomarkers of exercise effect. Twelve Polish warmblood horses underwent standardized exercise tests for six consecutive days. Both thermal images and blood samples were collected before and after each test. All 144 obtained IRT images were analyzed independently for 12 color components in four color models using eight texture-feature approaches, including 88 features. The similarity between blood biomarker levels and texture features was determined using linear regression models. In the horses' thoracolumbar region, 12 texture features (nine in RGB, one in YIQ, and two in HSB) were related to blood biomarkers. Variance, sum of squares, and sum of variance in the RGB were highly repeatable between image processing protocols. The combination of two approaches of image texture (histogram statistics and gray-level co-occurrence matrix) and two color models (RGB, YIQ), should be considered in the application of digital image processing of equine IRT. [ABSTRACT FROM AUTHOR]

Infrared thermography (IRT) was applied as a potentially useful tool in the detection of pregnancy in equids, especially native or wildlife. IRT measures heat emission from the body surface, which increases with the progression of pregnancy as blood flow and metabolic activity in the uterine and fetal tissues increase. Conventional IRT imaging is promising; however, with specific limitations considered, this study aimed to develop novel digital processing methods for thermal images of pregnant mares to detect pregnancy earlier with higher accuracy. In the current study, 40 mares were divided into non-pregnant and pregnant groups and imaged using IRT. Thermal images were transformed into four color models (RGB, YUV, YIQ, HSB) and 10 color components were separated. From each color component, features of image texture were obtained using Histogram Statistics and Grey-Level Run-Length Matrix algorithms. The most informative color/feature combinations were selected for further investigation, and the accuracy of pregnancy detection was calculated. The image texture features in the RGB and YIQ color models reflecting increased heterogeneity of image texture seem to be applicable as potential indicators of pregnancy. Their application in IRT-based pregnancy detection in mares allows for earlier recognition of pregnant mares with higher accuracy than the conventional IRT imaging technique. [ABSTRACT FROM AUTHOR]

Simple Summary: Overloading of the horse's thoracolumbar region is a serious problem mainly affecting sport and school horses during their daily under-saddle work. As the human population becomes heavier, the effect of rider bodyweight on equine welfare and performance requires further investigation. This study used infrared thermography to assess the effect of rider:horse bodyweight ratio on the horse's thoracolumbar region by introducing advanced digital image processing. Twelve horses during regular work were ridden by each of six riders assigned to light (L), moderate (M), and heavy (H) groups. Thermal images of the back region were taken before and after standard exercise and underwent conventional analysis and texture analysis where the thermal images were separated into red, green, and blue components. Four areas of the horse's thoracolumbar region were annotated to represent the withers area, the thoracic spine area, and the left and right areas of back muscles. Among 372 returned features, 75 texture features differed between bodyweight ratio groups, whereas the conventional thermal features did not. Contrary to conventional thermal features, the consistent measurable differences in texture features were evidenced predominantly in the red component of thermal images when the texture heterogeneity measures, such as InvDefMom, SumEntrp, Entropy, DifVarnc, and DifEntrp, were considered. Appropriate matching of rider–horse sizes is becoming an increasingly important issue of riding horses' care, as the human population becomes heavier. Recently, infrared thermography (IRT) was considered to be effective in differing the effect of 10.6% and 21.3% of the rider:horse bodyweight ratio, but not 10.1% and 15.3%. As IRT images contain many pixels reflecting the complexity of the body's surface, the pixel relations were assessed by image texture analysis using histogram statistics (HS), gray-level run-length matrix (GLRLM), and gray level co-occurrence matrix (GLCM) approaches. The study aimed to determine differences in texture features of thermal images under the impact of 10–12%, >12 ≤15%, >15 <18% rider:horse bodyweight ratios, respectively. Twelve horses were ridden by each of six riders assigned to light (L), moderate (M), and heavy (H) groups. Thermal images were taken pre- and post-standard exercise and underwent conventional and texture analysis. Texture analysis required image decomposition into red, green, and blue components. Among 372 returned features, 95 HS features, 48 GLRLM features, and 96 GLCH features differed dependent on exercise; whereas 29 HS features, 16 GLRLM features, and 30 GLCH features differed dependent on bodyweight ratio. Contrary to conventional thermal features, the texture heterogeneity measures, InvDefMom, SumEntrp, Entropy, DifVarnc, and DifEntrp, expressed consistent measurable differences when the red component was considered. [ABSTRACT FROM AUTHOR]