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Nile crocodiles (Crocodylus niloticus) at Lake St Lucia, South Africa, have some of the highest blood lead (Pb) concentrations ever recorded in wildlife globally. Although exposure to Pb is known to pose major risks to wildlife reproductive success, potential impacts on crocodile reproduction at Lake St Lucia have yet to be examined. In this study, we investigated the accumulation of Pb and other trace metals (Al, V, Cr, Co, Cu, Ni, Zn and Cd) in eggs (n = 20) collected from five wild crocodile nests at Lake St Lucia. All metals analysed in this study were detected in egg contents, although concentrations varied considerably among nests and within clutches. Lead was detected in the contents of all eggs, but only at relatively low concentrations (43 ± 26 ng g−1 dry weight). Although sampling limitations commonly associated with wild population surveys prevent a complete assessment of exposure variability, our findings suggest maternal transfer may not be a significant depuration pathway for Pb and females possibly clear Pb through other mechanisms (e.g. sequestration into claws, bone and osteoderms). Metal concentrations in eggshells and shell membranes were poorly correlated with concentrations measured in egg content and thus do not provide viable non-lethal indicators for monitoring metal exposure in Nile crocodiles. Intra-clutch variability accounted for a considerable proportion of the total variance in egg content metal concentrations, suggesting the "one egg" sampling strategy often applied in reptile studies may not be an effective biomonitoring tool for wild crocodilian populations. Although maternally derived Pb does not appear to present widespread toxicological concern at Lake St Lucia, adverse effects of Pb exposure on other reproductive functions (e.g. spermatogenesis) cannot be discounted and warrant further investigation. [ABSTRACT FROM AUTHOR]

Lead (Pb) exposure is a widespread wildlife conservation threat, but impacts on reptile populations remain poorly documented. In this study, we examined Pb exposure and accumulation in a wild population of Nile crocodiles (Crocodylus niloticus) at Lake St Lucia, South Africa. Recreational angling has occurred in the area since the 1930s and incidental ingestion of Pb fishing weights has previously been identified as a major source of Pb poisoning in the local crocodile population. In 2019, we sampled blood and tail fat tissues from wild (n = 22) and captive (n = 3) crocodiles at Lake St Lucia to investigate potential impacts of chronic Pb exposure on crocodilian health. Lead was detected in blood samples of all wild crocodiles, although concentrations varied widely between individuals (86–13,100 ng ml−1). The incidence of Pb poisoning was higher in male crocodiles, with mean blood lead (BPb) concentrations in males (3780 ± 4690 ng ml−1) significantly (p < 0.001) higher compared to females (266 ± 230 ng ml−1). Blood Pb concentrations were correlated with concentrations measured in tail fat tissue (n.d – 4175 ng g−1 wet wt.). Although most of the crocodiles sampled appeared to be in good physical condition, highly elevated BPb concentrations (>6000 ng ml−1) were associated with markedly suppressed packed cell volumes (4.6–10.8%) and severe deterioration in tooth condition. These findings suggest that anaemia and tooth loss may be clinical signs of long-term environmental exposure to Pb. Although previously undocumented in crocodilians, these symptoms are consistent with Pb poisoning observed in birds and mammals, and suggest that crocodilians may be more susceptible to the long-term toxic effects of Pb than previously thought. In light of these findings, we suggest that the impact of accumulated Pb on crocodilian fitness, reproduction and mortality requires urgent attention. [Display omitted] • We examine Pb concentrations in blood and fat tissues of free-ranging Nile crocodiles. • Highly elevated Pb levels attributed to incidental ingestion of Pb fishing weights. • Clinical signs of Pb toxicosis observed in individuals with blood Pb > 6000 ng ml−1 • Long-term Pb exposure associated with anaemia and severe tooth loss in individuals. [ABSTRACT FROM AUTHOR]