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Reproductive phasiRNAs (phased, small interfering RNAs) are broadly present in angiosperms and play crucial roles in sustaining male fertility. While the premeiotic 21-nt (nucleotides) phasiRNAs and meiotic 24-nt phasiRNA pathways have been extensively studied in maize (Zea mays) and rice (Oryza sativa), a third putative category of reproductive phasiRNAs-named premeiotic 24-nt phasiRNAs-have recently been reported in barley (Hordeum vulgare) and wheat (Triticum aestivum). To determine whether premeiotic 24-nt phasiRNAs are also present in maize and related species and begin to characterize their biogenesis and function, we performed a comparative transcriptome and degradome analysis of premeiotic and meiotic anthers from five maize inbred lines and three teosinte species/subspecies. Our data indicate that a substantial subset of the 24-nt phasiRNA loci in maize and teosinte are already highly expressed at the premeiotic phase. The premeiotic 24-nt phasiRNAs are similar to meiotic 24-nt phasiRNAs in genomic origin and dependence on DCL5 (Dicer-like 5) for biogenesis, however, premeiotic 24-nt phasiRNAs are unique in that they are likely i) not triggered by microRNAs, ii) not loaded by AGO18 proteins, and iii) not capable of mediating PHAS precursor cleavage. In addition, we also observed a group of premeiotic 24-nt phasiRNAs in rice using previously published data. Together, our results indicate that the premeiotic 24-nt phasiRNAs constitute a unique class of reproductive phasiRNAs and are present more broadly in the grass family (Poaceae) than previously known. [ABSTRACT FROM AUTHOR]

Fusarium head blight (FHB) resistance in wheat and triticale has a high priority in the European Union because of the strict guidelines for the major mycotoxins deoxynivalenol (DON) and zearalenone (ZON) and the admission policy of the regulatory authorities. Potentially 70% of the arable land in Germany and about 60% in Austria can be affected by Fusarium. Although epidemics occur only in some years and/or some regions, DON and ZON are detected every year in varying amounts in wheat, rye and maize. Despite a high significance of FHB resistance in breeding companies, as validated by a recent survey, breeding progress in wheat is basically absent for FHB resistance in both countries. The main reasons are the complex inheritance of FHB resistance and the high proportions of the dwarfing allele Rht‐D1b in high‐yielding varieties promoting susceptibility. Despite this, some varieties with high FHB resistance (score 2–3 on the 1–9 scale) have been released that account, however, only for 11% and 18% of the multiplication area in Germany and Austria, respectively. For triticale, an official testing system for FHB resistance in terms of DON content exists in Germany and Austria, but not for the other cereals. Susceptibility to maize ear rot has been described in Austria, but not in Germany. Additionally, a testing system for stalk rot resistance in both countries should be established. [ABSTRACT FROM AUTHOR]

Maintaining or even increasing crop yields while reducing nitrous oxide (N2O) emissions is necessary to reconcile food security and climate change, while the metric of yield‐scaled N2O emission (i.e., N2O emissions per unit of crop yield) is at present poorly understood. Here we conducted a global meta‐analysis with more than 6000 observations to explore the variation patterns and controlling factors of yield‐scaled N2O emissions for maize, wheat and rice and associated potential mitigation options. Our results showed that the average yield‐scaled N2O emissions across all available data followed the order wheat (322 g N Mg−1, with the 95% confidence interval [CI]: 301–346) > maize (211 g N Mg−1, CI: 198–225) > rice (153 g N Mg−1, CI: 144–163). Yield‐scaled N2O emissions for individual crops were generally higher in tropical or subtropical zones than in temperate zones, and also showed a trend towards lower intensities from low to high latitudes. This global variation was better explained by climatic and edaphic factors than by N fertilizer management, while their combined effect predicted more than 70% of the variance. Furthermore, our analysis showed a significant decrease in yield‐scaled N2O emissions with increasing N use efficiency or in N2O emissions for production systems with cereal yields >10 Mg ha−1 (maize), 6.6 Mg ha−1 (wheat) or 6.8 Mg ha−1 (rice), respectively. This highlights that N use efficiency indicators can be used as valuable proxies for reconciling trade‐offs between crop production and N2O mitigation. For all three major staple crops, reducing N fertilization by up to 30%, optimizing the timing and placement of fertilizer application or using enhanced‐efficiency N fertilizers significantly reduced yield‐scaled N2O emissions at similar or even higher cereal yields. Our data‐driven assessment provides some key guidance for developing effective and targeted mitigation and adaptation strategies for the sustainable intensification of cereal production. [ABSTRACT FROM AUTHOR]

The study of the supramolecular organization of the mitochondrial oxidative phosphorylation system (OXPHOS) in various eukaryotes has led to the accumulation of a considerable amount of data on the composition, stoichiometry, and architecture of its constituent superstructures. However, the link between the features of system arrangement and the biological characteristics of the studied organisms has been poorly explored. Here, we report a comparative investigation into supramolecular and functional OXPHOS organization in the mitochondria of etiolated shoots of winter wheat (Triticum aestivum L.), maize (Zea mays L.), and pea (Pisum sativum L.). Investigations based on BN-PAGE, in-gel activity assays, and densitometric analysis revealed both similarities and specific OXPHOS features apparently related to the life strategies of each species. Frost-resistant winter wheat was distinguished by highly stable basic I1III2IVa/b respirasomes and V2 dimers, highly active complex I, and labile complex IV, which were probably essential for effective OXPHOS adaptation during hypothermia. Maize, a C4 plant, had the highly stable dimers IV2 and V2, less active complex I, and active alternative NAD(P)H dehydrogenases. The latter fact could contribute to successful chloroplast–mitochondrial cooperation, which is essential for highly efficient photosynthesis in this species. The pea OXPHOS contained detergent-resistant high-molecular respirasomes I1–2III2IVn, highly active complexes IV and V, and stable succinate dehydrogenase, suggesting an active energy metabolism in organelles of this plant. The results and conclusions are in good agreement with the literature data on the respiratory activity of mitochondria from these species and are summarized in a proposed scheme of organization of OXPHOS fragments. [ABSTRACT FROM AUTHOR]

Maize and wheat are the main cereal forage crops cultivated at a global scale. They are among preferred forage grains for poultry nutrition. The aim of this study was to evaluate the productive performance of broiler chickens, whose compound feeds contained either maize, or wheat alone and maize +wheat combination. The experiment was performed with 120 day-old male ROSS hybrid chicks reared on the floor for 49 days. The birds were divided into three groups, each one consisting of two subgroups, as follows: group Е1 – fed forage with maize and wheat; group Е2 – fed forage with wheat and group Е3 – forage with maize. The live weight, feed intake, feed conversion and slaughter traits were investigated. By the 49 th day of age, the live weight of chickens from groups Е1 and Е2 fed diet with maize and wheat (35.99%+28%), and only wheat without maize (66%) were the heaviest, 3147.18 g and 3361.32 g, respectively. Chicks from group E3 fed the diet containing only maize (64.12%) without wheat had the lowest average live weight (2587.75 g at Р<0.0001). Wheat, as a single cereal in broiler compound feeds, and its combination with maize had the most beneficial effect on weight gain and feed conversion over the entire fattening period (P<0.001). The improved weight gain of birds fed these diets was accompanied with higher feed intake (P<0.001) compared to birds fed forage with maize only. The slaughter yield and the breast with bone cut increased statistically significantly in the wheat-fed groups (P<0.001). [ABSTRACT FROM AUTHOR]

Although accurate forecasting of the production of major cereal crops is important to make policy decisions related to their supply, demand and import/export, very little work has been done in Ethiopia. Therefore, this study aimed to develop the best performing trend models for area, production, and yield of wheat (Triticum aestivum L.), teff [Eragrostis tef (Zucc.) Trotter], and maize (Zea mays L.) in Ethiopia, and to provide forecasts for 2019 to 2023. For each crop, the best-performing model was selected among linear, quadratic, exponential growth, and S-curve trend models using five performance measures (mean absolute percent error [MAPE], mean absolute deviation [MAD], mean squared deviation [MSD], coefficient of efficiency [E], and index of agreement [d]). Quadratic trend model was the best for area of wheat and teff, production of wheat, teff, and maize, and wheat and maize yields. S-curve and Linear trend models were the best for area of maize and yield of teff, respectively. Forecasts for 2019 to 2023 using the best-performing model revealed that cultivated area for these three cereals will decrease by 3,581 ha in Ethiopia, but their combined total production will increase by 4,896,630 tons, and productivity of wheat, teff, and maize will increase by 20.3%, 13.2%, and 13.8%, respectively, which is slightly higher than the projected population growth (10.9%) during this time. Although these results indicate that Ethiopia is on track to meet the second SDG, government policymakers and other stakeholders can use these findings to ensure sustainable food and nutrition security in Ethiopia. [ABSTRACT FROM AUTHOR]

Maize and wheat are important stable food and cash crops in Ethiopia. However, the productivity and quality of these crops are highly limited by high weed infestations. Identification and quantification of weeds are very important. The survey was conducted to identify and document weed species in study areas. Therefore, the objectives were to determine the distribution of weeds, to document major cereal crops weed species and to identify newly introduced weeds in study areas. The study was carried out in Siltie zone (Sankura and Lanfuro districts) and Gurage zone (Mareko and Meskan districts) during 2022 cropping season. A total of 22 fields for each crop were randomly assessed within minimum of 3 kilometres distance. The selected field was assessed by using 0.5 m × 0.5 m quadrants. The assessment in farms was performed with a simple random sampling technique by throwing quadrants. There were three random sampling sites per field taken. The survey result revealed that thirteen weed families and twenty nine weed species were identified in maize field while fifteen weed families and thirty weed species in wheat field. The family Poaceae, Asteraceae and Solanaceae were dominant families in both maize and wheat fields. The frequency (0.05 up to 0.95%) and the dominance (0.09% up to 38.55%) of weed species were recorded in maize field, while the frequency (0.05 up to 0.95%) and the dominance (0.05% up to 57.81) of weed species in wheat field. Galinsoga parviflora, Phalaris minor and Guzotia scabra weeds in maize field and Galinsoga parviflora, Avena fatua, Guzotia scabra, Phalaris minor and Cyperus species weeds in wheat field were the most frequent and dominant weeds in four selected districts. [ABSTRACT FROM AUTHOR]

Cover crops (CC) have great potential to enhance the sustainability of agroecosystems. However, the wide range of possible rotations of CC and cash crops (CaC) means that important knowledge gaps persist on how CC affects CaC. We investigated the legacy effects of five common CC (three monocultures: vetch, melilotus, and barley, and two mixtures: barley-vetch and barley-melilotus) on two of the most important CaC, maize and wheat. A microcosm, semi-controlled experiment was established simulating warm, low-income Mediterranean conditions. After two cycles, soil physicochemical and microbial properties, as well as plant growth and nutrition variables, were measured at the CC early growth CaC stage. In maize, barley CC had the best soil microbial and nutritional legacy effects, which resulted in the highest biomass and nutrient status. In contrast, barley produced the worst results on wheat, showing the disadvantages of growing two crops from the same tribe consecutively. CC mixtures also did not offer a productive advantage over pure CC. Additionally, our findings suggest that archaea seem to play a role in increasing N and Zn content in maize shoots. Furthermore, shoot B contents showed highly significant regressions with the CaC biomass. These results can help select the appropriate CC in each case. [ABSTRACT FROM AUTHOR]

The paper purpose was to assess the impact of climate change on wheat, maize and sunflower yield in the period 2017-2021, using statistical data from National Institute of Statistics (NIS) and National Administration of Meteorology (NAM) and other sources. Comparison method was used to evaluate the deviations between the registered air temperatures and precipitations and the 1981-2010 climatological norm. Graphic method reflected the dynamics of monthly air temperatures and rainfalls in each year. Descriptive statistics for mean, standard deviation and coefficient of variation reflected an more comprehensive image upon air temperature, precipitations and yield. Correlations and regression equations were used to establish the intensity of the kinks between climate factors and yield. The highest average air temperature In Romania was 12.130C in 2019. In the period 2017-2020, the average annual temperatures exceeded the climatological norm 1981-2010 accounting for 9.10C. The lowest rainfalls, 614.2 mm, were recorded in 2019, being below of 633.1 mm the norm for the period 1981-2010. The high temperatures associated with drought and low precipitations have deeply affected yield of many agricultural crops, including maize, wheat and sunflower. The highest wheat yield 4,888 kg/ha was achieved in 2017, and the lowest one, 2,966 kg/ha in 2020. Maize registered the highest performance of 7,644 kg/ha in 2018, and the lowest one, 3,977 kg/ha in 2020. Sunflower carried out the high performance 3,041 kg/ha in 2018, and the lowest one, 1,858 kg/ha in 2020. The worst agricultural years for these three crops was 2020, but 2017 favored wheat, while 2018 favored maize and sunflower. The correlation coefficient had in general small values between average temperature and yield, but a higher link with precipitations level. The multiple correlation between yield, air temperatures and precipitations was: r= 0.817 for wheat, r = 0.116 for maize and r = 0.504 for sunflower. In the South Eastern Dobrogea, the driest area in Romania, the rainfalls declined by 65% in the period 2018-2020 having a deep negative impact on sunflower seeds yield, which decreased from 4,282 kg/ha in 2018, to 1,503 kg/ha in 2020. The correlation between precipitations and sunflower yield positive and very strong (r = 0.737). As a conclusion, the climate conditions should be analyzed in each farm and farmers have to take measure to adapt the technologies for sustaining production. [ABSTRACT FROM AUTHOR]

The aim of present study was to investigate the potential of indigenous plant growth promoting bacteria (PGPB) for enhancing the nutrient availability and plant growth. Field experiment was conducted and total three sampling sites [Bhimber (BH), Samahni (SA) and Barnala (BA)] were selected. Samples were collected from fallow fields under long term (about 70 years) cultivation of wheat (Triticum aestivum) and maize (Zea mays). Soil nutrients and physicochemical properties were determined and climatic data was obtained from the nearest metrological station. Total 26 different isolates were obtained from the soils, out of which 10 were from SA while 8 each from BH and BA soils. The highest amount of IAA (44.8 µg/mL), siderophore production (12.83 µg/mL), N-fixation (128.6 µg/mL) and P-sol (40.01 µg/mL) was recorded in SA as compared to BH and BA soils while Ksol (13.61 µg/mL) and Znsol (28.2 µg/mL) was highest in BA. Bacillus spp. showed maximum IAA production (52.01 µg/mL), Ksol (19.1 µg/mL) and ZnSol (34.64 µg/mL). The maximum GA (1371 µg/mL), siderophore production (17.22 µg/mL) and Psol (62.64 µg/mL) was observed by Pseudomonas spp. The production and solubilization of some important plant growth hormones and nutrients were highest in SA by Bacillus, Rhizobium, Pseudomonas, BH by Pseudomonas and BA by Bacillus. It was concluded that PGPB have potential to promote growth of plants and nutrient regulation. [ABSTRACT FROM AUTHOR]

Background: Lifestyle diseases are ailments caused due to unhealthy living pattern. Maize being an affordable and inexpensive cereal crop for unprivileged people and has potential benefits also which can be utilized with other grains to develop functional flour. Methods: The present investigation was conducted during 2020-21 in the Laboratory of Department of Food and Nutrition, Dr. RPCAU. The raw ingredients viz. maize (variety 'Lakshmi'), flaxseed, chickpea, barley, ragi and wheat were procured from local market Pusa or farmers. After processing with various methods, different proportions of flour were developed. Bulk density, water absorption capacity and proximate composition were determined. Result: The result showed that bulk density ranged from 0.57 to 0.67 g/ml, whereas water absorption capacity varied from 1.08 to 2.16 g/ml. Proximate composition such as moisture content ranged from 6.53 to 11.55 g/100 g, ash 1.26 to 1.9 g/100 g, protein 5.83 to 11.08 g/100 g, fat 1.60 to 5.33 g/100 g, fibre 11.09 to 18.27 g/100 g, carbohydrate 56.45 to 65.09 g/100 g whereas level of minerals like iron varied from 3.68 to 5.71 mg/100 g, zinc 2.83 to 3.76 mg/100 g and calcium 25.73 to 132.28 mg/100 g. [ABSTRACT FROM AUTHOR]

High temperature inhibits the growth and development of maize (Zea mays L.) and wheat (Triticum aestivum L.) seedlings. The current study attempts to elucidate the underlying mechanisms of exogenous trehalose for the improvement of maize and wheat responses to heat stress. Results show that, in maize seedlings, exogenously-supplied trehalose was able to effectively elevate the capacity of photosynthetic carbon assimilation and improve the carbohydrate content via up-regulating the transcript levels of key enzymes including phosphoenol pyruvate carboxylase (PEPC), NADP-malate dehydrogenase (NADP-MDH), NADP-malic enzyme (NADP-ME), pyruvate phosphate dikinase (PPDK) and Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit. Besides, it was also able to improve C4 pathway enzyme activities, including PEPC, NADP-MDH, NADP-ME and Ribulose-1,5-bisphosphate carboxylase (RUBPCase). In wheat seedlings, exogenously-supplied trehalose promoted photosynthetic carbon assimilation by elevating the activity of RUBPCase. These results show that exogenous trehalose improved photosynthetic carbon assimilation capacities in maize and wheat seedlings under heat stress through different mechanisms. [ABSTRACT FROM AUTHOR]

The water footprint (WF), based on irrigation water quality, is important as a decision-making tool for crop selection based on the comparative advantage of water consumption and yield to maximize agricultural water productivity and sustainably improve water use efficiency. This paper presents a generic link between climate variability and water footprint. To support this link, a case study is presented for wheat and maize in different agro-climate zones in Egypt. In this study, the three agro-ecological zones, Nile Delta, Middle Egypt, and Upper Egypt, were selected to represent three different microclimates. The climate data were analyzed to estimate reference evapotranspiration (ETo) and calculate crop water use (CWU) for wheat and maize from 2015 through 2019. Cultivated area and yield data were analyzed during the study period. Water footprint (WF) was calculated for old land (clay soils) and new lands (sandy soils) in three climate regions based on blue and grey water. Green water was excluded due to negligible rainfall depths in Egypt. The results showed that the mean values of WF for maize were 1067, 1395, 1655 m3/ton in old land and 1395, 1634, 2232 m3/ton in new land under the three climate regions, respectively, while it was 923, 982, 1117 m3/ton in old land and 1180, 1258, 1452 m3/ton for wheat in new land for the three regions, respectively. The results show that the crop water use fluctuated over regions due to climate variability where the CWU values were 6211, 7335, 8007 m3/ha for maize and 4348, 4825, 5774 m3/ha for wheat in the three regions, respectively. The results show an 11% and 33% increase in maize and an 18% and 29% increase in wheat CWU in Middle and Upper Egypt regions comparing to what was observed in Nile Delta due to an increase in solar radiation, temperature, and wind speed. The Egypt mean value of wheat water footprint was 1152 m3/ton and mean value of maize water footprint was 1563 m3/ton. The data clearly show the effect of microclimate variability on WF and irrigation requirements between regions. The methodology and results from this study provide a pathway to help the policy makers to mitigate climate change impacts on crop yield and to enhance water resources management in major crop production regions by redistribution of the cropping patterns based on the comparative advantages of each crop within each region. The crop choices relative to the soil water retention characteristics could also contribute to the moderation of microclimate, which affects ETo and ETc and the water footprint. [ABSTRACT FROM AUTHOR]

In recent years, worldwide scientific efforts towards controlling maize late wilt disease (LWD) have focused on eco-friendly approaches that minimize the environmental impact and health risks. This disease is considered to be the most severe threat to maize fields in Israel and Egypt, and a major growth restraint in India, Spain, and Portugal. Today's most commonly used method for LWD control involving resistant maize genotypes is under constant risk from aggressive pathogen lines. Thus, this study's objectives were to evaluate the effect of crop rotation and avoiding tillage on restraining the disease. Such an agrotechnical approach will support the continuity of soil mycorrhiza networks, which antagonize the disease's causal agent, Magnaporthiopsis maydis. The method gained positive results in previous studies, but many knowledge gaps still need to be addressed. To this end, a dual-season study was conducted using the LWD hyper-susceptible maize hybrid, Megaton cv. The trials were performed in a greenhouse and in the field over full dual-growth seasons (wheat or clover as the winter crop followed by maize as the summer crop). In the greenhouse under LWD stress, the results clearly demonstrate the beneficial effect of maize crop rotation with clover and wheat on plant weight (1.4-fold), height (1.1–1.2-fold) and cob yield (1.8–2.4-fold), especially in the no-till soil. The clover-maize growth sequence excels in reducing disease impact (1.7-fold) and pathogen spread in the host tissues (3-fold). Even though the wheat-maize crop cycle was less effective, it still had better results than the commercial mycorrhizal preparation treatment and the uncultivated non-infected soil. The results were slightly different in the field. The clover-maize rotation also achieved the best growth promotion and disease restraint results (2.6-fold increase in healthy plants), but the maize rotation with wheat showed only minor efficiency. Interestingly, pre-cultivating the soil with clover had better results in no-till soil in both experiments. In contrast, the same procedure with wheat had a better impact when tillage was applied. It may be concluded that crop rotation and soil cultivation can be essential in reducing LWD, but other factors may affect this approach's benefits in commercial field growth. [ABSTRACT FROM AUTHOR]

The mechanical properties of the materials stored in agricultural silos determine the loads they generate under static and dynamic conditions. The present work describes the mechanical properties of wheat, maize and wood pellets. Direct shear and triaxial assay devices, and oedometers (all commonly used in geotechnical assays), were used to determine these materials' internal angle of friction, Poisson's ratio, Young's modulus, apparent specific weight, etc. The results for wheat and maize were similar to those previously reported by other authors. For the wood pellets, the results for the internal angle of friction and apparent specific weight were also similar to those found in the literature. However, this is a relatively new type of material, and few results of this type have been reported, certainly not enough for reference values to be available. This work is the first to report this material's dilatancy angle and Poisson's ratio. A table is provided with suggested reference values for the studied mechanical properties of each of the test materials; these can be used in silo load calculations involving numerical methods. [ABSTRACT FROM AUTHOR]

Concerns about the effects of global warming on crop yields have been raised due to stagnating yield progress in recent years. However, an understanding of the effects of changes in temperature and rainfall throughout the crop cycle on historical yield progress is lacking in Europe (EU). In this study, positive wheat, barley, rice, and maize yield progress in the EU (1961–2019) was significant, with rates of 0.05, 0.04, 0.05, and 0.07 Tha−1year−1, for the four crops respectively. Much of this progress has been sustained by Eastern European countries (EE), which had the highest yield progress rates. On average, in the case of wheat and barley, a temperature increase of 1°C in the winter resulted in yields increasing by +0.33 Tha−1 in EE. This was potentially due to decreasing cold damage and improved photosynthesis and vegetative growth, supporting positive yield progress. Recent historical (2001–2019) rates of wheat, barley, rice, and maize yield progress were positive in all EU regions except Western Europe (WE), barley in Southern Europe (SE) and wheat in Northern Europe (NE). Stagnated wheat, barley and rice yields in WE were not explained by temperature or rainfall using direct correlations of observed data. However, May and July temperatures were associated with wheat yields in NE (−0.30 Tha−1°C−1), barley in SE (−0.14 Tha−1°C−1) and maize in WE and SE (−0.42 and −0.39 Tha−1°C−1). With increasing temperatures becoming less than optimal for photosynthesis, reducing grain filling duration and increasing drought episodes, crop yields have stagnated for wheat in NE and barley in SE. With consistent increases in temperature and water evaporative demand expected in the future, the interplay among genetic adaptation, increased crop cycle duration, drought tolerance, sowing dates, smart irrigation and sustainable practices may require thorough regional testing to maximise the yields of wheat, barley and maize in Europe. [ABSTRACT FROM AUTHOR]

Maize and millet are the other major cereal grains grown worldwide along with wheat grains and their incorporation in products prepared solely with wheat flours can alter nutritional and sensorial attributes of products positively. Present study has been designed to develop multigrain muffins by utilizing wheat, maize and millet in different proportions i.e., T0 (100% wheat flour), T1 (80% wheat flour, 10% maize and millet flour each), T2 (60% wheat flour, 20% maize and millet flour each), T3 (40% wheat flour, 30% maize and millet flour each), T4 (20% wheat flour, 40% maize and millet flour each), T5 (50% maize and millet flour each), T6 (100% maize flour) and T7 (100% millet flour) treatments have been prepared and investigated for their nutritional profile and sensorial characteristics (performa evaluated on 9 points Hedonic scale). Moreover, storage study of the products was carried on 0, 10, 20 and 30th day of preparation. Data has been collected and subjected to twoway ANOVA by using software Statistics 8.1. The overall mean values for crude protein (6.97%) and crude fiber (1.17%) contents proved to be improved in T3. On the other hand, T3 proved to be good in attaining best sensory scores in aroma and texture. Overall acceptability of T3 remained in good condition on average basis throughout storage period of muffins. So, it has been concluded that using wheat flour in combination with the maize flour and millet flour not only improves the nutritional profile of products but also the sensorial attributes. This product would be a valuable healthy addition in the market for consumers if studied further for launching and commercialization. [ABSTRACT FROM AUTHOR]

Background: Nicotianamine (NA), 2′-deoxymugineic acid (DMA), and mugineic acid (MA) are chelators required for iron uptake and transport in plants. Nicotianamine aminotransferase (NAAT), 2′-deoxymugineic acid synthase (DMAS), transporter of MAs (TOM), and efflux transporter of NA (ENA) are involved in iron uptake and transport in rice (Oryza sativa), wheat (Triticum aestivum), and barley (Hordeum vulgare); however, these families have not been fully identified and comprehensively analyzed in maize (Zea mays L.). Results: Here, we identified 5 ZmNAAT, 9 ZmDMAS, 11 ZmTOM, and 2 ZmENA genes by genome mining. RNA-sequencing and quantitative real-time PCR analysis revealed that these genes are expressed in various tissues and respond differently to high and low iron conditions. In particular, iron deficiency stimulated the expression of ZmDMAS1, ZmTOM1, ZmTOM3, and ZmENA1. Furthermore, we determined protein subcellular localization by transient expression of green fluorescent protein fusions in maize mesophyll protoplasts. ZmNAAT1, ZmNAAT-L4, ZmDMAS1, and ZmDMAS-L1 localized in the cytoplasm, whereas ZmTOMs and ZmENAs targeted to plasma and tonoplast membranes, endomembranes, and vesicles. Conclusions: Our results suggest that the different gene expression profiles and subcellular localizations of ZmNAAT, ZmDMAS, ZmTOM, and ZmENA family members may enable specific regulation of phytosiderophore metabolism in different tissues and under different external conditions, shedding light on iron homeostasis in maize and providing candidate genes for breeding iron-rich maize varieties. [ABSTRACT FROM AUTHOR]

The use of plant growth‐promoting bacteria (PGPB) to enhance plant growth and protection against heavy metal toxicity has been extensively studied. However, its potentiality to reduce arsenate toxicity, a threat to plant growth and metabolism, has been hardly investigated. Moreover, the toxic effect of arsenic oxide nanoparticles (As‐NPs) on plants and possible mechanisms for its alleviation has not yet been explored. In this study, the impact of the bioactive actinomycete Streptomyces spp. on the growth, physiology and stress‐related metabolites, such as sugars and proline, on As‐NPs‐stressed wheat and maize plants was investigated. Soil amendment with arsenic oxide nanoparticles (As‐NPs) induced the uptake and accumulation of As in the plants of both species, resulting in reduced growth and photosynthesis, but less marked in maize than in wheat plants. Under As‐NPs‐free conditions, Streptomyces spp. treatment markedly improved growth and photosynthesis in wheat only. The application of Streptomyces spp. reduced As accumulation, recovered the As‐NPs‐induced growth, photosynthesis inhibition, and oxidative damage in plants of both species. Wheat plants specifically accumulated soluble sugars, while both species accumulated proline. Under As‐NPs stress, the ornithine pathway of proline biosynthesis was more important in maize than in wheat plants, while the glutamine pathway was dominant in wheat ones. The addition of Streptomyces spp. further induced the accumulation of proline and starch in both plant species. Overall, despite a different response to Streptomyces spp. under nontoxic conditions, the amendment of as‐contaminated soil with Streptomyces spp. induced similar metabolic responses in the two tested species, which trigger stress recovery. [ABSTRACT FROM AUTHOR]

GA 2-oxidases (GA2oxs) are a class of enzymes that inhibit the biosynthesis of bioactive GAs in plants. Although GA2oxs have clear roles in the development and defence responses in Arabidopsis, rice, and wheat, their potential effects on maize remain unclear. This study identified thirteen ZmGA2ox genes in maize and further characterized them using phylogenetic, gene structure, genomic locus, expression pattern analyses and GA content determination. Phylogenetic relationship analysis clearly divided the ZmGA2ox family into three groups—seven in C19-GA2ox class I, three in C19-GA2ox class II, and three in C20-GA2ox class. Evolutionary analysis suggested that ZmGA2ox1;1 and ZmGA2ox1;2 , ZmGA2ox3;1 and ZmGA2ox3;2 , and ZmGA2ox7;1 and ZmGA2ox7;2 are three pairs of segmental duplicated genes. Prediction of cis-regulatory elements in promoters suggested that ZmGA2ox genes were mainly associated with growth, development, hormones, and biotic/abiotic stress. Therefore, their spatial and temporal expression patterns and responses to various stress treatments were analysed on the basis of published RNA-seq data. Moreover, the changes of ZmGA2ox expression in leaves and roots of maize seedlings was detected under salt, alkali, dehydration, and cold stresses by qRT-PCR. The ZmGA2oxs exhibited obvious expression tendencies or characteristics in various organs under different abiotic stresses. The variations in the expression of three ZmGA2ox genes in the C20-GA2ox class in maize seedling roots showed significant regularity and a clear negative correlation with bioactive GA contents under cold and drought conditions, indicating that these three genes might exert key effects on the regulation of GA synthesis and the response to drought and cold stress. Taken together, this study is useful for further dissection of the effect of ZmGA2oxs on abiotic stress responses and might provide potential targets for the genetic improvement of maize. • A set of 13 ZmGA2ox genes were identified in maize via genome-wide screening. • Spatio-temporal specificity of ZmGA2ox gene expression was accurately demonstrated. • C20-GA2ox expressions in roots showed opposite changes under the cold and PEG stress. [ABSTRACT FROM AUTHOR]

Development of semi-dwarf wheat (Triticum aestivum) and rice (Oryza sativa) led to increased yields and agronomic efficiencies with less lodging. While these short-stature cereals have become common in global crop production, commercial maize (Zea mays L.) hybrids remain tall. There is no information currently available on plant N uptake or yield responses to N timing for short stature maize hybrids. We tested three hypotheses on short-stature and tall maize hybrids 1) short-stature maize hybrids would have similar yield response to in-season N application compared to tall hybrids, 2) short-stature maize hybrids would have similar dry matter and N uptake patterns to tall hybrids, and 3) there would be significant partitioning differences between short-stature and tall hybrids in dry matter and N within the plants at multiple growth stages. In ten side-by-side field trials across eight site-years in the Midwestern USA, we tested the yield response and underlying nitrogen physiology of short-stature maize hybrids and tall comparators to split-rate N application. We show that split-rate application of 50% of the total N at mid-vegetative stages (V6) averaged 0.24 t ha−1 more grain yield across 10 field trials (positive yield response in 6 out of 10 trials) compared to a single 100% up-front N application, with the same pattern for short-stature and tall hybrids. In both hybrid statures, V6 N applications showed more frequent improvement in grain yield than V12 N applications. Total N uptake at maturity was not different between short-stature hybrids and their tall counterparts (p = 0.62). N partitioning differed between short-stature and tall hybrids; leaves in short-stature hybrids had 5% higher N concentration and 13% more N content in early vegetative development, stalks had 18–27% less dry matter and 5–25% less N content, depending on growth stage, while R1 ears averaged 18% more dry matter and N accumulation (p < 0.10 in all cases). At maturity short-stature hybrids had 4% higher harvest index and 3% higher N harvest index (p < 0.10). Based on these results, we expect that N management recommendations in short-stature maize hybrids would be similar to their tall counterparts, but that short stature mitigates logistical risk by providing more flexibility for in-season N applications. This study provides a unique, robust dataset on yield and physiological response to split-rate N treatments in commercially relevant maize hybrids. • Short stature in maize simplifies in-season N application and later crop management. • Split-rate N applications at V6 increased hybrid yields 60% of the time, with similar patterns for short-stature and tall hybrids. • Overall, short-stature maize yielded similarly to tall comparators. • Short stature led to greater partitioning of N and dry matter to the grain than tall hybrids. • Greater flexibility for in-season N fertilization in short-stature maize reduces in-season application risks. [ABSTRACT FROM AUTHOR]

Yield-scaled nitrous oxide (N 2 O) emission (YSNE) has been recognized as a means for developing appropriate nitrogen (N) management strategies to balance food security and mitigating N 2 O emissions. To better understand and use the concept of YSNE, it is essential to be assessed under various field conditions. The main objectives of this study were to assess the relationship between N inputs and YSNE with published results and identify response patterns of YSNE to N inputs. We assessed the relationship between N inputs and YSNE using published results encompassing 1800 observations (published from 1980 to 2020) from maize, rice, and wheat crops worldwide. Background yield-scaled N 2 O emission (BYSNE; a YSNE in the condition of zero N input) was significantly different by crop type. Rice (90.8 ± 12.9 g N 2 O−N Mg−1) had lower BYSNE compared to maize (174.2 ± 30.1 g N 2 O−N Mg−1) and wheat (325.4 ± 41.8 g N 2 O−N Mg−1). BYSNE was positively correlated with annual mean temperature in maize, rice, and wheat fields. BYSNE was negatively correlated with soil total nitrogen contents in rice fields. Over 60% data set showed a positive relationship between N inputs and YSNE in all three crops studied. A small proportion of the dataset had an optimum N rate that minimized YSNE. The results suggest that in general, lower N input rates result in lower YSNE in crop production. YSNE can be reduced in three ways: increasing yields (Type 1), reducing N 2 O emissions (Type 2), and both increasing yields and reducing N 2 O emissions (Type 3). To identify appropriate measures, we suggest a N 2 O emission-yield curve combining responses of yields and N 2 O emissions to N inputs. In type 1, 2, and 3 measures, an N 2 O emission-yield curve is shifted to the right, down, and both right and down, respectively. Through identifying direction and magnitude of the shifting, the effects of applied measures on yields and N 2 O emission in N input levels can be easily compared and recognized. This study provided insights into the nature of YSNE and how YSNE responds to N inputs. It also suggested an N 2 O emission-yield curve, which can be useful to identify how certain measures affect both N 2 O emissions and crop yields. The information can be useful for scientific community and policymakers developing appropriate N management strategies to balance food security and the mitigation of N 2 O emissions. [Display omitted] • Background yield-scaled N 2 O emission (BYSNE) varied by crop type. • BYSNE was positively correlated with annual mean temperature. • Over 60% data sets showed consistently lower yield-scaled N 2 O emission as N input decreased. • N 2 O emission yield curve showed the effects of mitigation measures on both yields and N 2 O emission. [ABSTRACT FROM AUTHOR]

MiR396s play important roles in regulating plant growth and stress response, and great potential for crop yield promotion was anticipated. For more comprehensive and precise understanding of miR396s in Poaceae, we analyzed the phylogenetic linkage, gene expression, and chromosomal distribution of miR396s in this study. Although the mature miR396s' sequences were mostly conserved, differential expression patterns and chromosomal distribution were found among Poaceae species including the major cereal crops rice, wheat, and maize. Consistently, in comparison with rice, wheat and maize plants transformed with the target mimicry construct of miR396 (MIM396) exhibited differential effects on grain size and disease resistance. While the TaMIM396 plants showed increased grain size, panicle length and sensitivity to B. graminis , the ZmMIM396 plants didn't show obvious changes in grain size and disease resistance. In Addition, several GROWTH-REGULATING FACTOR (GRF) genes in wheat and maize were repressed by miR396s, which could be reversed by MIM396, confirming the conserved regulatory roles of miR396 on GRFs. While providing new solution to enhance grain yield in wheat and revealing potential regulatory variations of miR396s in controlling grain size and disease resistance in different crops, this study gives clues to further explore miR396s' functions in other Poaceae species. • Poaceae miR396s are divided into three groups and display both conserved and diversified evolutionary characterizations. • Inhibition of miR396s results in increased grain size, panicle length and pathogen sensitivity in wheat but not in maize. • Different GRFs were upregulated in the MIM396 transgenic plants compared to their corresponding wild type in wheat and maize. [ABSTRACT FROM AUTHOR]

• We determined frequency of environment types (ENVT) for US maize, soybean, and wheat. • Each ENVT corresponded to a combination of drought and heat stress during critical period. • Inter-annual variation in drought and heat stress was higher than spatial variation. • The ENVT scheme explained observed variation in average actual yield for the three crops. • ENVTs can be used to link field-year experiments with target production environments. The impact of agricultural technologies on crop yield is influenced by the environment type (ENVT) as determined by weather and soil. Understanding the correlation between the ENVT of the testing site in relation to the ENVT of the target production region is important for the evaluation and scaling out of agricultural technologies. Here we propose and apply the first explicit method to characterize ENVTs for major rainfed maize, soybean, and wheat producing regions in the United States. We combined a tested spatial framework, Technology Extrapolation Domain (TED), with crop modeling, long-term (30-y) daily weather records, and soil and management databases to calculate the frequency of ENVTs per crop for major harvested areas. Each ENVT was determined based on the intensity of drought and heat stress during key crop stages for yield determination. The ENVT repeatability was calculated based on the frequency of the most dominant ENVT in each TED. We found that inter-annual variation in drought and heat stress was larger than spatial variation. Our ENVTs explained 2x to 7x larger portion of the variance in actual yield compared to the existing TED framework that is based on long-term annual climate means and soil water storage. For maize and soybean, ca. 30% of their harvested area was located in TEDs with highly repeatable ENVTs (>66% of years). In contrast, only 15% of the wheat harvested area was located in TEDs with high ENVT repeatability. In comparison to the TED framework, the ENVTs defined here can help better capture G×E×M interactions and determine the environmental correlation between testing sites and target production environments. [ABSTRACT FROM AUTHOR]