1. Paeonol inhibits human lung cancer cell viability and metastasis in vitro via miR-126-5p/ZEB2 axis
- Author
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Shibing Zhu, Ying Xu, Jing Lv, Guofen Yu, Qingyu Su, Wei Ma, and Huiping Chen
- Subjects
Lung Neoplasms ,Cell Survival ,Metastasis ,Transforming Growth Factor beta1 ,chemistry.chemical_compound ,Western blot ,Cell Movement ,Cell Line, Tumor ,Drug Discovery ,microRNA ,medicine ,Gene silencing ,Humans ,Neoplasm Invasiveness ,Viability assay ,Epithelial–mesenchymal transition ,Lung cancer ,Cell Proliferation ,Zinc Finger E-box Binding Homeobox 2 ,medicine.diagnostic_test ,Chemistry ,Acetophenones ,medicine.disease ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Cancer research ,Paeonol - Abstract
Paeonol exerted an effect in lung cancer, but the underlying mechanism remained vague. In this research, we assessed the effects of Paeonol and microRNA (miR)-126-5p on the viability, migration, invasion, and epithelial-mesenchymal transition (EMT) of lung cancer cells. Lung cancer cells and BEAS-2B cells were treated with Paeonol, and viability was detected by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide (MTT) assay. The migration and invasion of lung cancer cells after treatment with Paeonol at 40 μg/mL or 80 μg/mL were detected by wound healing assay and Transwell assay, respectively. The effects of Paeonol on transforming growth factor-β1 (TGF-β1)-induced EMT and relative expressions of EMT-related proteins were determined using Western blot. The target gene of miR-126-5p and the binding sites between them were predicted by TargetScan, and confirmed using dual-luciferase reporter assay. Relative expressions of miR-126-5p, its target gene and EMT-related proteins were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Rescue assay was performed to analyze the relation between Paeonol and miR-126-5p. Paeonol down-regulated cell viability and inhibited migration, invasion and TGF-β1-induced EMT while up-regulating miR-126-5p expression in lung cancer cells as the dose increased. However, miR-126-5p inhibitor could reverse the effect of Paeonol. ZEB2 was the target gene of miR-126-5p, and silencing ZEB2 expression reversed the effects of miR-126-5p downregulation. Paeonol also regulated the expression of ZEB2 in lung cancer cells, and this regulation depends on the regulation of miR-126-5p. Paeonol inhibits human lung cancer cell viability and metastasis via the miR-126-5p/ZEB2 axis, and could be adopted as a potential agent for lung cancer treatment.
- Published
- 2021