151. A paper-based sensor for rapid and ultrasensitive detection of ibuprofen in water and herbal tea
- Author
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Shaozhen Li, Liqiang Liu, Wenliang Ge, Jianping Zhu, Jie Liu, Chuanlai Xu, Hua Kuang, and Steven Suryoprabowo
- Subjects
Chromatography ,Chemistry ,food and beverages ,Water ,Ibuprofen ,Paper based ,Biochemistry ,Chromatography, Affinity ,Analytical Chemistry ,Herbal tea ,Specific antibody ,Linear range ,Limit of Detection ,Electrochemistry ,medicine ,Environmental Chemistry ,Naked eye ,Teas, Herbal ,Spectroscopy ,medicine.drug - Abstract
As the use of non-steroidal anti-inflammatory drugs (NSAIDS) increases, their side effects have also attracted attention. Ibuprofen is one of the most widely-used NSAIDs. In this study, we screened the highly-sensitive and specific antibody 6E10, with an IC50 of 1.92 ng mL-1, and a linear range of 0.53-6.97 ng mL-1. In this study, we developed a rapid lateral flow immunochromatographic assay (ICA) strip method to detect ibuprofen in water or herbal tea. The cut-off limit of the strip is 10 ng mL-1 in water, and concentrations as low as 1 ng mL-1 can be detected in herbal tea samples, with the results obtained by the naked eye within 6 min. All the data were confirmed by high performance liquid chromatography-quadrupole time of flight-mass spectrometry (HPLC-QTOF-MS). This lateral-flow ICA strip is thus a rapid tool for on-site detection and screening of ibuprofen in water and herbal tea.
- Published
- 2021