1. Longitudinal IgA and IgG Response, and ACE2 Binding Blockade, to Full-Length SARS-CoV-2 Spike Protein Variants in a Population of Black PLWH Vaccinated with ChAdOx1 nCoV-19.
- Author
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Smith, Muneerah, Kwatra, Gaurav, Izu, Alane, Nel, Andrew, Cutland, Clare, Ahmed, Khatja, Baillie, Vicky, Barnabas, Shaun, Bhorat, Qasim, Briner, Carmen, Lazarus, Erica, Dheda, Keertan, Fairlie, Lee, Koen, Anthonet, Madhi, Shabir, and Blackburn, Jonathan M.
- Subjects
ANGIOTENSIN converting enzyme ,IMMUNOGLOBULIN G ,VIRAL antibodies ,IMMUNOGLOBULIN A ,FC receptors ,SARS-CoV-2 ,BLOCKADE ,ANGIOTENSIN I - Abstract
Vaccines against SARS-CoV-2 have been pivotal in overcoming the COVID-19 pandemic yet understanding the subsequent outcomes and immunological effects remain crucial, especially for at-risk groups e.g., people living with human immunodeficiency virus (HIV) (PLWH). In this study we report the longitudinal IgA and IgG antibody titers, as well as antibody-mediated angiotensin converting enzyme 2 (ACE2) binding blockade, against the SARS-CoV-2 spike (S) proteins after 1 and 2 doses of the ChAdOx1 nCoV-19 vaccine in a population of Black PLWH. Here, we report that PLWH (N = 103) did not produce an anti-S IgA response after infection or vaccination, however, anti-S IgG was detected in response to vaccination and infection, with the highest level detected for infected vaccinated participants. The anti-IgG and ACE2 blockade assays revealed that both vaccination and infection resulted in IgG production, however, only vaccination resulted in a moderate increase in ACE2 binding blockade to the ancestral S protein. Vaccination with a previous infection results in the greatest anti-S IgG and ACE2 blockade for the ancestral S protein. In conclusion, PLWH produce an anti-S IgG response to the ChAdOx1 nCoV-19 vaccine and/or infection, and ChAdOx1 nCoV-19 vaccination with a previous infection produced more neutralizing antibodies than vaccination alone. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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