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Identifying traits for adaptation to different management and environmental regimes is key to maintaining robust honey bee populations under global climate change. We compared mite-resistant (Pol-line and Russian) and Italian honey bee stocks in variable-temperature cage experiments (200 bees per cage) with respect to food consumption, thermoregulation, gene expression, and lifespan, in 3 experiments over 2 years. The Italian stock bees consumed more syrup and pollen on average than the mite-resistant stocks, but the mite-resistant stocks maintained higher cluster temperatures and had median lifespans 8 days longer, consistent with the increased expression of vitellogenin relative to Italian stock. Model results indicated that, to maintain the same colony size as the mite-resistant stocks, Italian stock colonies would need about 13% more sealed brood to offset reduced worker lifespans. These differences among bee stocks likely influence colony-level productivity and health, and showed the importance of experimental replication. [ABSTRACT FROM AUTHOR]

Keywords: neonicotinoid; sublethal effects; colony-level behavior; thermoregulation; bee cage study EN neonicotinoid sublethal effects colony-level behavior thermoregulation bee cage study N.PAG N.PAG 11 09/27/22 20220901 NES 220901 1. The focus of this study was the effect of exposure of flonicamid on honey bee colony behavior in a field experiment and on honey bee survivorship and thermoregulation in controlled cage studies, as has been observed with those other pesticides mentioned above. The total adult bee population weight was calculated by subtracting the combined weights of hive components free of bees from the total hive weight with bees recorded at midnight prior to the inspection. [Extracted from the article]

CO2, a byproduct of respiration, is toxic at high concentrations so regulation of CO2 within the honey bee hive is an important colony function. In this study, we measured hive CO2 concentrations at 1-s intervals while ventilation characteristics of the hive were changed every few days, and we analyzed the data for effects of increased ventilation on colony behavior and thermoregulation. Average CO2 concentrations were significantly higher, by > 200 ppm, when hives had screened bottom boards (higher ventilation) compared to hives with solid bottom boards (lower ventilation) at the same time. Daily CO2 concentration amplitudes, hourly temperature, daily temperature amplitudes, nor hourly hive weight changes were not significantly affected by the changes in hive ventilation. In a second experiment, we found average CO2 concentrations at the top center of the upper hive box, on top of the frames, were significantly lower than concentrations at the center of a solid bottom board underneath frames, which was expected due to the higher density of CO2 relative to air. Bee colonies have been reported to cycle air, with shorter periods of 20 to 150 s and longer periods of 42–80 min, but a periodogram analysis of the CO2 concentration data found no evidence of important CO2 cycle periods other than a strong 24-h period. Bee colonies maintained strong daily cycles of CO2 concentration, with average maximum concentrations > 11,000 ppm, even in conditions of increased ventilation, indicating that managing CO2 concentration is a complex colony behavior. [ABSTRACT FROM AUTHOR]

Placing honey bee colonies in cold storage has been proposed as a way to induce a pause in brood production as part of a Varroa mite treatment plan. Here, we exposed colonies to combinations of with or without an October cold storage period and with or without a subsequent miticide application. We then measured the effects of those treatments on colony-level variables (i.e. colony size, Varroa infestation level, survivorship and hive weight and temperature) and pooled individual-level variables that are associated with nutritional and stress responses. Colonies were assessed before and after cold storage, and again post winter, for a total duration of about 5 months, and the experiment was repeated. Brood levels were significantly lower after cold storage, and hive temperatures indicated that most or all brood had emerged after about two weeks in cold storage. However, Varroa levels at the end of the experiments in February were not significantly different among treatment groups. Colonies kept outside (not subjected to cold storage) and treated with a miticide had higher survivorship on average than any other treatment group, but no other group comparisons were significant, and long-term impact of cold storage on adult bee populations and on colony thermoregulation was low. The bee forage environment was also very different between the 2 years of the study, as rainfall and bee forage availability were much higher the second year. Colonies were over 2.5 times larger on average the second year compared to the first, both in terms of adult bee mass and brood area, and expression levels of nutrition and stress response genes were also significantly higher the second year. The results indicate that limited cold storage would likely have little long-term impact on most colony and individual measures of health, but for such a strategy to succeed levels of stressors, such as Varroa, may also need to be low. [ABSTRACT FROM AUTHOR]

The relationship between beehive weight and traffic is a fundamental open research problem for electronic beehive monitoring and digital apiculture, because weight and traffic affect many aspects of honeybee (Apis mellifera) colony dynamics. An investigation of this relationship was conducted with a nondisruptive two-sensor (scale and camera) system on the weight and video data collected on six Apis mellifera colonies in Langstroth hives at the USDA-ARS Carl Hayden Bee Research Center in Tucson, Arizona, USA, from 15 May to 15 August 2021. Three hives had positive and two hives had negative correlations between weight and traffic. In one hive, weight and traffic were uncorrelated. The strength of the correlation between weight and traffic was stronger for longer time intervals. The traffic spread and mean, when taken separately, did not affect the correlation between weight and traffic more significantly than the exact traffic counts from videos. Lateral traffic did not have a significant impact on weight. [ABSTRACT FROM AUTHOR]