1. A rhodamine derivative-based fluorescent probe for visual monitoring of pH changes in the Golgi apparatus.
- Author
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Feng, Zhongjiao, Wu, Jiasheng, Jiang, Meiyu, Sha, Jie, Liu, Weimin, Ren, Haohui, Zhang, Wenjun, Lee, Chun-Sing, and Wang, Pengfei
- Subjects
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GOLGI apparatus , *FLUORESCENT probes , *CELL physiology , *CELLULAR therapy , *FLUORESCENCE - Abstract
The pH homeostasis of Golgi plays a vital role in maintaining normal cell functions. An effective near-infrared fluorescent probe that can sense pH changes in Golgi apparatus is of great significance. Here, a Golgi-targeting fluorescent probe (Golgi-hNR) based on a rhodamine derivative is presented to show a highly sensitive response to pH variations. Golgi-hNR displays a conspicuous pH-dependent behavior at 664 nm from pH 7.4–3.0 with a remarkable fluorescence off-on response. The pKa value of Golgi-hNR is measured to be 3.62. Confocal fluorescence imaging reveals that Golgi-hNR diffuses into Golgi with high selectivity. Using Golgi-hNR , we have successfully visualized real-time tracking of Golgi pH variations for medical therapy in living cells. • An effective near-infrared fluorescent probe (Golgi-hNR) based on rhodamine is presented to sense pH variations. • Golgi-hNR displays a conspicuous pH-dependent behavior with a remarkable fluorescence off-on response. • Golgi-hNR was successfully visualized for real-time tracking of Golgi pH variations for medical therapy in cells. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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