1. Optimization of differentiation conditions for porcine adipose-derived mesenchymal stem cells and analysis of fatty acids in cultured fat.
- Author
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Liu, Pei-Pei, Yang, Zi-Jiang, Song, Wen-Juan, Ding, Shi-Jie, Li, Hui-Xia, and Li, Chun-Bao
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FATTY acid analysis , *LIQUID chromatography-mass spectrometry , *UNSATURATED fatty acids , *STEM cell culture , *MESENCHYMAL stem cells - Abstract
[Display omitted] • A combination of differentiation-inducing factors is obtained in order to produce high-quality cultured fat by single-factor experiment and orthogonal experiment. • Cultured fats with collagen as scaffolds are successfully prepared. • Cultured fats contain forty-eight fatty acids, mainly C16:0, C18:0, C20:0, C16:1, C18:1n-9, C18:1n-7, C20:4, C22:5n-3, and C22:6, and cultured fat and porcine subcutaneous fat are similar in fatty acid composition. • The ratio of ω-6 fatty acids content/ω-3 fatty acids content in cultured fat is 1.23:1, which is good and recommended for human health. Cultured fat is an important part of cultured meat, and the ability of adipose-derived mesenchymal stem cells (ADSCs) to differentiate into mature adipose tissue affects the quality of cultured fat. Thus, the primary aim of this study was to screen for combinations of differentiation-inducing factors (DIF) using single-factor experiment and orthogonal experimental design under two-dimensional culture conditions for ADSCs. The results showed that a combination of DIF consisting of 1 μmol/L dexamethasone, 0.1 mmol/L 3-isobutyl-1-methylxanthine, 10 μg/mL insulin, 0.1 mmol/L indomethacin, and 2 μmol/L rosiglitazone was a good choice for the differentiation of ADSCs. An combination of DIF was applied to the preparation of cultured fat with collagen as scaffolds. Forty-eight fatty acids were detected in cultured fat by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Among them, the content of twenty-one fatty acids in cultured fat was significantly higher than that of conventional porcine subcutaneous adipose tissue (P < 0.05), and the content of 14 fatty acids was not significantly different (P > 0.05). The ratio of ω-6 polyunsaturated fatty acids content to ω-3 polyunsaturated fatty acids content was 1.23:1, which meant cultured fat was beneficial for human health. This study provides a method to improve the differentiation ability of ADSCs while also providing a reference for indicating the nutritional value of cultured fat. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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