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[Objectives] To study the main active components, targets and related pathways of Ningmitai capsule for the treatment of urinary tract infections (UTIs) based on network pharmacology. [Methods] The chemical components of Ningmitai capsule were collected through literature search, and the relevant target information of the components was sorted out. The UTIs-associated targets were also screened out using DisGeNET database and GeneCards database. Cytoscape 3.6.1 software and STRING platform were used to construct the protein-protein interaction (PPI) network, and MCODE plug-in in this software was used to analyze the action pathway and key targets of Ningmitai capsule for the treatment of UTIs. GO and KEGG pathway enrichment analysis of key targets was conducted using David database, and the component-target-pathway network diagram of Ningmitai capsule for the treatment of UTIs was established. [Results] A total of 37 active compounds, including salicylate, ferulic acid, baicalin, quercetin, apigenin and ellagic acid were screened from seven TCM components of Ningmitai capsule. There were 26 possible targets related to the treatment of UTIs, such as NFKB1, JUN, CTNNB1 and STAT3, which play an important role for the treatment of UTIs through prostate cancer, bladder cancer, pancreatic cancer and other signaling pathways. [Conclusions] The study provides a theoretical basis for the study of the mechanism of Ningmitai capsule in the treatment of UTIs. [ABSTRACT FROM AUTHOR]

[ Objectives ] To investigate the inhibitory effect and possible mechanism of essential oil from Valerianae Jatamansi Rhizoma et Radix on microglia activation induced by lipopolysaccharide $ LPS ). [ Methods ] The LPS-induced microglia activation model was established and treated with different doses of essential oil from Valerianae Jatamansi Rhizoma et Radix. MTT assay was used to detect cell viability, ELISA to detect IL-6 secretion, RT-PCR to detect mRNA expression levels of IL-6, IL-1, NF-kB, and IkBcc, Western blotting to detect the protein expression of IL-6, IL-1,NF-kB, IkBcc, and their phosphorylated products. [ Results] Compared with the norma control group, the model group showed increased IL-6 content $ A<0.01), upregulated mRNA and protein levels of IL-6, IL-1", and NF-kB $ A<0.01), and elevated ratio of P-IkBcc/IkBcc $ A < 0. 05). Compared with the model group, 4 and 2 xg /L essential oil from Valerianae Jatamansi Rhizoma et Radix reduced the content of IL-6 $ A < 0. 05), while 4, 2, and 1 xg /L essential oil from Valerianae Jatamansi Rhizoma et Radix down-regulated the mRNA and protein levels of IL-6, IL-1 and NF-kB to varying degrees $ A < 0. 05 or A < 0. 01), up-regulate the mRNA expression of IkBcc $ A <0. 05 or A <0.01), and decreased the ratio of P-IkBcc/IkBcc $ A <0.05 or A <0.01). [ Conclusions] Essential oil from Valerianae Jatamansi Rhizoma et Radix can inhibit LPS-induced microglia activation, and its mechanism may be related to the inhibition of the NF-kB/IkBcc signaling pathway. [ABSTRACT FROM AUTHOR]