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Inflammatory response in the pulmonary endothelium drives the pathogenesis of acute lung injury and sepsis. Sirtuin 6 (SIRT6), a member of class III NAD+-dependent deacetylases belonging to the sirtuin family, regulates senescence, metabolism, and inflammation and extends lifespan in mice and model organisms. However, the role of SIRT6 in pulmonary endothelial inflammation is unknown. Thus, we hypothesized that SIRT6 suppresses inflammatory response in human lung microvascular cells (HLMEC) and ensues monocyte adhesion to endothelial cells. Primary HLMECs were treated with control or SIRT6 adenovirus or SIRT6 agonist, with or without lipopolysaccharide (LPS) treatment. We observed that treatment with LPS did not affect the protein expression of SIRT6 in HLMECs. However, adenovirus-mediated SIRT6 overexpression attenuated LPS-induced VCAM1 gene and protein expression, followed by decreased monocyte adhesion to endothelial cells. Similarly, activation of SIRT6 by a recently reported SIRT6 activator UBCS039, but not the regioisomer negative control compound UBCS060, ameliorated LPS-induced VCAM1 mRNA and protein expression as well as monocyte adhesion. Moreover, luciferase assay revealed that SIRT6 adenovirus decreased the activity of NF-κB, the master regulator of vascular inflammation. Taken together, these results indicate that molecular and pharmacological activation of SIRT6 protects against lung microvascular inflammation via suppressing NF-κB activation, implicating the therapeutic potential of the SIRT6 activators for lung disorders associated with microvascular inflammation. [ABSTRACT FROM AUTHOR]

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Iron carbon agglomerates (ICA) are used to realize low-carbon blast furnace ironmaking. In this study, the central composite design based on response surface methodology was used to synergistically optimize the compressive strength, reactivity, and post-reaction strength of ICA. Results show that the iron ore addition ratio significantly influences the compressive strength, reactivity, and post-reaction strength of ICA. The iron ore addition ratio and carbonization temperature or the iron ore addition ratio and carbonization time exert significant interaction effects on the compressive strength and reactivity of ICA, but it has no interaction effects on the post-reaction strength of ICA. In addition, the optimal process parameters are as follows: iron ore addition ratio of 15.30wt%, carbonization temperature of 1000°C, and carbonization time of 4.27 h. The model prediction results of compressive strength, reactivity, and post-reaction strength are 4026 N, 55.03%, and 38.24%, respectively, which are close to the experimental results and further verify the accuracy and reliability of the models. [ABSTRACT FROM AUTHOR]