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Due to recent advancements in connected and autonomous vehicles (CAVs), freeway traffic control (FTC) has become a trendy area of research. Combining CAVs and control measures has opened more efficient traffic flow possibilities. However, while there is extensive research on how traffic control and CAVs can enhance traffic flow performance, their safety benefits should be evaluated more comprehensively. It is challenging to distinguish their contributions to traffic flow safety. Studies on the safety of CAVs in traffic flow indicate that traffic flow safety improves with higher market penetration rates (MPR) of CAVs. However, this finding only sometimes aligns with the traffic flow performance effects of CAVs on freeways. This paper introduces a framework for assessing the safety effects of FTC strategies in mixed traffic scenarios involving human-driven vehicles and CAVs. The proposed framework is tested through a microsimulation-based case study in Istanbul, Turkey. The results show that, despite varying MPRs, the safety effects of CAVs and FTC methods do not consistently reduce the number of conflicts in the traffic flow context. [ABSTRACT FROM AUTHOR]