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Mol Med Rep 17: 4817-4822, 2018; DOI: 10.3892/mmr.2018.8449 Subsequently to the publication of this paper, an interested reader drew to the authors' attention that Figs. The authors have consulted their original data, and realized that the control western blot images were inadvertently selected incorrectly for Fig. [Extracted from the article]

TSHZ3 siRNA alone or combined with miR-125b-1-3p inhibitor was administered to NP cells and the expression of (A) COL2, (B) TSHZ3 and (C) miR-125b-1--p was measured from different generations of NP cells. Exp Ther Med 13:2627-2633, 2018; DOI: 10.3892/etm.2018.5715 After the publication of the above article, the authors have realized that the -actin control bands were selected incorrectly for Figs. [Extracted from the article]

Osteoporosis is a common metabolic disease that has a high incidence in postmenopausal women. Studies have indicated that oxidative damage plays an important role in the development of postmenopausal osteoporosis. Metformin has been showed to have the ability to relieve excessive oxidation. The aim of the present was to determine the therapeutic effect and potential mechanism of metformin in postmenopausal osteoporosis. Oxidative damage was stimulated in vitro by the addition of H2O2 to MC3T3-E1 cells and a mouse menopausal model was also constructed. Cell viability and flow cytometry experiments were performed to determine the effects of H2O2 and metformin treatment on apoptosis. Mitochondrial membrane potential was tested by JC-1 assays. Western blotting was used to detect the expression of mitochondrial apoptosis markers and antioxidant enzymes. Small interfering RNA was used to knockdown sirtuin3 (SIRT3), which was verified at the mRNA and protein levels. Bilateral ovariectomy was used to prepare menopausal mice, which were analyzed using micro-computed tomography. The results indicated that metformin is able to repair mitochondrial damage and inhibit the apoptosis of osteoblasts induced by H2O2, and also reverse bone mass loss in ovariectomized mice. Western blotting results demonstrated the involvement of SIRT3 in the production of antioxidant enzymes that are essential in protecting against mitochondrial injury. In addition, experiments with SIRT3 knockdown indicated that metformin reverses H2O2-induced osteoblast apoptosis by upregulating the expression of SIRT3 via the PI3K/AKT pathway. The results of the present reveal the pathogenesis of oxidative damage and the therapeutic effect of metformin in postmenopausal osteoporosis. They also suggest that SIRT3 is a potential drug target in the treatment of osteoporosis, with metformin being a candidate drug for modification and/or clinical application. [ABSTRACT FROM AUTHOR]