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At present, percutaneous nephrolithotomy has become an option for hospitals increasingly to treat calculus. However, due to the certain risk of percutaneous nephrolithotomy, it may lead to postoperative complications. Analyzing the preoperative factors of septic shock after percutaneous nephrolithotomy can provide guidance for reducing the incidence of septic shock after PCNL. This article establishes a logistic regression model based on preoperative factors, uses statistical methods, and uses the professional statistical software SPSS to create a database and analyze the data. The method of classification data analysis was used to determine various influencing factors including patient factors, stone factor, and preoperative factors and combined B-ultrasound guidance and percutaneous nephrolithotomy to reduce the risk of surgery. The experimental results found that the percutaneous nephroscope laser lithotripsy guided by the smart Internet of Things can effectively perform lithotripsy; in this paper, the preoperative data of different complications are obtained, and the risk of surgery is closely related to the operation time. The operation time exceeds 90 minutes and causes the risk of severe bleeding after PCNL. It is twice the operation time of less than 90 minutes and 5 times the operation time of less than 60 minutes. [ABSTRACT FROM AUTHOR]

Organic matter (OM) and carbonate are the important organic and inorganic components, respectively, in the shales of the upper fourth member of the Eocene Shahejie Formation, Dongying Depression, Eastern China. Their enrichment affects the hydrocarbon generation capacity and reservoir development of shales. The development mechanism of sequence stratigraphy and the influence of sequence stratigraphy on OM enrichment and carbonate deposition are investigated in this study. Based on the petrological, mineralogical, and geochemical analyses, a third-order sequence is recognized, including the lowstand systems tract (LST), transgressive systems tract (TST), and highstand systems tract (HST). The total organic carbon (TOC) content is 0.11–9.05 wt% (average 2.2 wt%), and the carbonate content is 3–95 wt% (average 51 wt%). Organic matter and carbonate are enriched in TST and gradually reduced in HST and LST. The decrease in the relative lake level in LST results in the deposition of abundant siltstone, leading to both poor OM preservation and carbonate precipitation. The rapid rise in the relative lake level in TST may be triggered by marine transgression, promoting OM enrichment in terms of both production and preservation. Seawater input brought abundant Ca2+ and Mg2+, which facilitated the precipitation of carbonates. The enrichment of OM and carbonate improves the capacity of hydrocarbon generation and reservoir performance of shales in the TST. The decrease in the relative lake level in HST causes a slight increase in the terrigenous clastic content, which, on the one hand, accelerates the sedimentation rate and OM deposition and, on the other hand, reduces carbonate precipitation. This study not only explains the differential distribution of OM and carbonate but also helps to improve the accuracy of the evaluation of shale oil sweet spots. [ABSTRACT FROM AUTHOR]

Organic matter (OM) and carbonate are the important organic and inorganic components, respectively, in the shales of the upper fourth member of the Eocene Shahejie Formation, Dongying Depression, Eastern China. Their enrichment affects the hydrocarbon generation capacity and reservoir development of shales. The development mechanism of sequence stratigraphy and the influence of sequence stratigraphy on OM enrichment and carbonate deposition are investigated in this study. Based on the petrological, mineralogical, and geochemical analyses, a third-order sequence is recognized, including the lowstand systems tract (LST), transgressive systems tract (TST), and highstand systems tract (HST). The total organic carbon (TOC) content is 0.11–9.05 wt% (average 2.2 wt%), and the carbonate content is 3–95 wt% (average 51 wt%). Organic matter and carbonate are enriched in TST and gradually reduced in HST and LST. The decrease in the relative lake level in LST results in the deposition of abundant siltstone, leading to both poor OM preservation and carbonate precipitation. The rapid rise in the relative lake level in TST may be triggered by marine transgression, promoting OM enrichment in terms of both production and preservation. Seawater input brought abundant Ca2+ and Mg2+, which facilitated the precipitation of carbonates. The enrichment of OM and carbonate improves the capacity of hydrocarbon generation and reservoir performance of shales in the TST. The decrease in the relative lake level in HST causes a slight increase in the terrigenous clastic content, which, on the one hand, accelerates the sedimentation rate and OM deposition and, on the other hand, reduces carbonate precipitation. This study not only explains the differential distribution of OM and carbonate but also helps to improve the accuracy of the evaluation of shale oil sweet spots. [ABSTRACT FROM AUTHOR]

Connotative development of higher education is of great significance in China. To promote this endeavor, this paper has established a connotative teaching system based on the Kano model and has applied it in the teaching of statistics. Guided by the fundamental demands of students, this system is built from the four perspectives, including students, third-party evaluation institutions, schools, and morality and law. It also takes the advantages of the Kano model, featuring the three dimensions of essential demand quality, expected demand quality, and charismatic demand quality. The paper also describes a two-year experiment to test the effect of the Kano-model-based connotative teaching system. It has collected more than 200 effective samples and compared the performance of students in private colleges. The results are as follows: the connotative teaching of statistics guided by the Kano model can meet the demands of students in private colleges and improve their comprehensive scores. This model shows remarkable performance and will promote the reform on connotative teaching. [ABSTRACT FROM AUTHOR]

Currently, PRRSV-1 causes a large number of clinical infections in Chinese swine herds, and the prevalence of new strains has presented great challenges. In this study, the novel PRRSV-1 strain SL-01 was isolated, with a genome length of 14,978 bp, and genetic evolution analysis revealed that it belonged to a new subtype branch. Sequence homology analysis showed that the strain was only 82.2%–86.7% identical to the current classical PRRSV-1 strains. In particular, the novel strain exhibited a unique deletion pattern in Nsp2. In addition, GP3 and GP4 of the SL-01 strain showed four consecutive amino acid deletions in the highly variable regions at amino acids 243–248 and 63–68, respectively. Further challenges in piglet and pregnant sow demonstrated that the SL-01 strain could cause the piglet fever and death but less pathogenic to pregnant sow. Overall, the characterization and pathogenicity of a novel PRRSV-1 strain were first explored and provide a prevention for pig farms. [ABSTRACT FROM AUTHOR]

Background. With the technology of high-fidelity simulates developed, the clinical education route has changed. The nursing school pays attention on the use of high-fidelity simulations in nursing education. However, in China, only a few schools can afford the expensive teaching tools, including SimMom 3G and virtual reality (VR) devices, which also focus on "holistic nursing simulation." Objective. To explore the evaluation and development of a holistic nursing simulation session for nursing students based on an integrated nursing approach in obstetrics. Methods. This study was based in a rich Chinese nursing school under the medical university that value nursing education. This study is founded on 147 third-year nursing students in obstetrics classes. After the simulation teaching, the teaching effect of the questionnaire was investigated, and the total number of questionnaires was 124 students. Results. Students agreed that the holistic nursing simulation cultivated humanistic care literacy, clinical practice ability, and clinical thinking ability; enhanced teamwork ability; and reinforced professional knowledge. Conclusion. This simulation teaching method helps to cultivate students' enthusiasm and initiative and fosters their self-learning ability. [ABSTRACT FROM AUTHOR]

Acacetin (5,7-dihydroxy-4′-methoxyflavone) is the major bioactive component of the traditional Chinese medicine "Snow lotus". As a natural flavonoid compound, it has been shown to have good pharmacological effects such as anti-inflammatory, anticancer, and anti-obesity. Among them, its prominent role in cardiovascular diseases (CVD) has received extensive attention from scholars in recent years. In this review, the protective effects of acacetin on a variety of cardiovascular diseases, as well as the existing problems and prospects, are discussed and summarized. This review also highlights the great potential of acacetin, a natural-derived Chinese medicine, as a cardiovascular agent candidate. [ABSTRACT FROM AUTHOR]

High dietary protein input in aquaculture leads to suboptimal growth and increased production costs. Limited knowledge on dietary protein requirements of Procambarus clarkii in pond culture has hindered the scientific expansion of optimum feeding strategies. We evaluated the effects of reduced dietary protein levels (from 30% to 26%) on the growth performance (weight, length, GSI, HSI, SGRw, SGRL and muscle weight) and muscle composition (crude protein, lipid, ash and moisture) of juveniles in eight concrete ponds cultured with Hydrilla verticillata. No significant differences were observed in the growth performance between the two treatments. P. clarkii fed a diet containing 26% protein had significantly higher crude protein and ash contents, whereas no significant differences existed in the lipid contents. The δ13C and δ15N values of P. clarkii and its food resources were not significantly affected by dietary protein levels. However, the Bayesian mixing model identified the mean contribution of H. verticillata in P. clarkii growth increased from 37.34% to 41.93% when dietary protein levels decreased from 30% to 26%. Considering the significant contribution of natural foods, we encourage the reduction in dietary protein levels from 30% to 26% in ponds to further reduce the production costs. [ABSTRACT FROM AUTHOR]

Background. The global impact of coronavirus 2019 (COVID-19) has raised concerns about the management of psoriasis patients, especially among those using biologics. Methods. We conducted a survey-based research among Omicron-infected (confirmed, probable, and suspected ones) psoriasis patients in the department of dermatology, West China Hospital, Sichuan University, from January 9th to January 22nd, 2023. We collected demographic and clinical information (psoriasis- and COVID-19-related) and conducted statistics analysis. Results. Of the 240 patients enrolled, they were classified by the psoriatic treatment, as biologics (n = 138), nonbiological systematic treatment (n = 52), and topical treatment or without pharmacological treatment (n = 50). This study showed the characteristics of Omicron-related symptoms and cutaneous signs in patients. We observed that patients who received topical treatment or without pharmacological treatment had a lower risk of presenting with COVID-19 symptoms in the fully adjusted logistic model (OR = 0.40, 95% CI: 0.18–0.90, and P = 0.025). Moreover, in the model for skin manifestations, nonbiological systematic treatment (OR = 2.15, 95% CI: 1.08–4.27, and P = 0.029) and high BMI (OR = 1.10, 95% CI: 1.00–1.20, and P = 0.042) were correlative factors. Conclusions. Our data suggested that differential psoriatic treatment might be a correlative factor in developing symptomatic or asymptomatic Omicron infection and presenting cutaneous signs. [ABSTRACT FROM AUTHOR]

Objective. Preeclampsia (PE) is a severe complication in pregnancy and a leading cause of maternal and infant mortality. However, the exact underlying etiology of PE remains unknown. Emerging evidence indicates that the cause of PE is associated with genetic factors. Therefore, the aim of this study is to identify susceptibility genes to PE. Materials and Methods. Human Exome BeadChip assays were conducted using 370 cases and 482 controls and 21 loci were discovered. A further independent set of 958 cases and 1007 controls were recruited for genotyping to determine whether the genes of interest ROS1 and PTPRK are associated with PE. Immunohistochemistry was used for localization. Both qPCR and Western blotting were utilized to investigate the levels of PTPRK in placentas of 20 PE and 20 normal pregnancies. Results. The allele frequency of PTPRK rs3190930 differed significantly between PE and controls and was particularly significant in severe PE subgroup and early-onset PE subgroup. PTPRK is primarily localized in placental trophoblast cells. The mRNA and protein levels of PTPRK in PE were significantly higher than those in controls. Conclusion. These results suggest that PTPRK appears to be a previously unrecognized susceptibility gene for PE in Han Chinese women, and its expression is also associated with PE, while ROS1 rs9489124 has no apparent correlation with PE risk. [ABSTRACT FROM AUTHOR]