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'Cumulative cultural evolution' broadly describes the process by which cultural traits accumulate improvements to efficacy over generations of social learning and innovation. The term can arguably be applied without much controversy to the cultural evolution of tool use, for example. However, it has recently gained momentum in the aesthetic realm as well, where it has been used to describe changes to sexually selected cultural traits. These, the argument goes, can gain efficacy in the sense of evoking stronger emotional responses from receivers. Here, we use examples from birdsong literature to outline our objections to the application of cumulative cultural evolution to cultural traits that achieve popularity based on no standard other than social or sexual preference. Moreover, we distinguish between categories of cultural traits that are preferred for different functional reasons, presenting arguments for or against describing changes to each as improvements. We conclude by proposing that a detailed and nuanced understanding of the mechanisms and outcomes of cultural change is of more service to behavioural and cultural science than a fraught binary distinction between what we can and cannot label 'cumulative'. • We summarize current definitions of 'cumulative cultural evolution' (CCE). • We suggest areas where CCE may apply to socially learned vocal communication. • We use birdsong to explore the untenability of applying CCE in the aesthetic realm. • More nuanced distinctions are needed between cultural traits than CCE can provide. [ABSTRACT FROM AUTHOR]

Morphogenesis of the nervous system involves a highly complex spatio-temporal pattern of physical forces (mainly tension and pressure) acting on cells and tissues that are pliable but have an intricately organized cytoskeletal infrastructure. This review begins by covering basic principles of biomechanics and the core cytoskeletal toolkit used to regulate the shapes of cells and tissues during embryogenesis and neural development. It illustrates how the principle of 'tensegrity' provides a useful conceptual framework for understanding how cells dynamically respond to forces that are generated internally or applied externally. The latter part of the review builds on this foundation in considering the development of mammalian cerebral cortex. The main focus is on cortical expansion and folding – processes that take place over an extended period of prenatal and postnatal development. Cortical expansion and folding are likely to involve many complementary mechanisms, some related to regulating cell proliferation and migration and others related to specific types and patterns of mechanical tension and pressure. Three distinct multi-mechanism models are evaluated in relation to a set of 18 key experimental observations and findings. The Composite Tension Plus (CT+) model is introduced as an updated version of a previous multi-component Differential Expansion Sandwich Plus (DES+) model (Van Essen, 2020); the new CT+ model includes 10 distinct mechanisms and has the greatest explanatory power among published models to date. Much needs to be done in order to validate specific mechanistic components and to assess their relative importance in different species, and important directions for future research are suggested. • Mechanical tension mediated by the cytoskeleton plays a key role in many aspects of morphogenesis. • Expansion and folding of cerebral cortex are key to achieving a large cortical surface area while keeping wiring compact. • The Composite Tension Plus (CT+) model invokes 10 distinct mechanisms to account for cortical expansion and folding. [ABSTRACT FROM AUTHOR]

• Data support EEG as an important tool to measure delirium-relevant aspects in mice. • During critical illness mice showed slow-wave EEG dominance and sleep disruption. • Hypersensitivity to auditory stimuli during sepsis can be measured in sick mice. • Cognitive disruption was observed in some mice following recovery from systemic inflammation. • Deficits post sepsis associated with altered hippocampal long term potentiation. Sepsis and systemic inflammation are often accompanied by severe encephalopathy, sleep disruption and delirium that strongly correlate with poor clinical outcomes including long-term cognitive deficits. The cardinal manifestations of delirium are fluctuating altered mental status and inattention, identified in critically ill patients by interactive bedside assessment. The lack of analogous assessments in mouse models or clear biomarkers is a challenge to preclinical studies of delirium. In this study, we utilized concurrent measures of telemetric EEG recordings and neurobehavioral tasks in mice to characterize inattention and persistent cognitive deficits following polymicrobial sepsis. During the 24-hour critical illness period for the mice, slow-wave EEG dominance, sleep disruption, and hypersensitivity to auditory stimuli in neurobehavioral tasks resembled clinical observations in delirious patients in which alterations in similar outcome measurements, although measured differently in mice and humans, are reported. Mice were tested for nest building ability 7 days after sepsis induction, when sickness behaviors and spontaneous activity had returned to baseline. Animals that showed persistent deficits determined by poor nest building at 7 days also exhibited molecular changes in hippocampal long-term potentiation compared to mice that returned to baseline cognitive performance. Together, these behavioral and electrophysiological biomarkers offer a robust mouse model with which to further probe molecular pathways underlying brain and behavioral changes during and after acute illness such as sepsis. [ABSTRACT FROM AUTHOR]

Lead (Pb2+) ions, among heavy toxic metals, are extensively distributed and bioaccumulate in food chains, prompting researchers to monitor their levels in water and biosystems. Consequently, aiming to protect the environment and human health, this study synthesizes a straightforward naphthalene-naphthalene dyad-based "turn-on" fluorescent chemosensor (S) tailored for detecting Pb2+ ions. This selective response to Pb2+ ions over other competing metal ions is characterized by hypsochromic changes in fluorescence enhancement resulting from an intramolecular charge transfer (ICT) process from -OH group (donor) to the naphthyl ring (acceptor) following the complexation of S-Pb2+. Moreover, cross-contamination titration for probe S was conducted in the presence of various distinct competitive metal ions. By employing Job's plot and B-H plot, the complicated stoichiometry (1:2) and association constant (K a = 9.45×104 M−1) of S-Pb2+ were studied. The probe's interaction with Pb2+ is studied using spectroscopic measurements and DFT calculations. The probe demonstrated exceptional selectivity and sensitivity, achieving a limit of detection (LOD) value of 12.50×10−6 M for Pb2+ in a buffered THF:H 2 O (50:50, v:v) medium. The applicability of chemosensor S spans across various water, food, and E. coli samples, indicating its potential in monitoring and managing Pb2+ toxicity in wastewater and biosystems. [Display omitted] • The study reports sensor S, detecting toxic Pb2+ ions rapidly and applicable in food, water, and living cells. • The sensor demonstrated high selectivity and sensitivity via ICT mechanistic process. • The detection limit (LOD) of 12.50 µM for Pb2+ is lower than many previous reports. • The sensor's versatility is showcased by its applicability in both biological and environmental contexts. [ABSTRACT FROM AUTHOR]

Background: Phase I trial to determine the safety and efficacy of paclitaxel, sapanisertib, and serabelisib. Patients and methods : Patients with previously treated advanced solid tumors were eligible for this open label, cohort study of sapanisertib (TAK-228) and serabelisib (TAK-117) with weekly paclitaxel. A traditional 3 + 3 dose escalation design with 5 dosing cohorts was used. Patient reported outcomes were also evaluated. Results: 19 heavily pretreated patients were enrolled (10 ovarian, 3 breast, and 6 endometrial cancers). All patients received comprehensive genomic profiling prior to enrollment. RP2D is sapanisertib 3 or 4 mg, serabelisib 200 mg on days 2–4, 9–11, 16–18 and 23–25 with paclitaxel 80 mg/m2 on days 1, 8 and 15 every 28 days. All patients in Cohort 5 required dose reductions and one patient experienced a DLT. The most frequent grade 3 or 4 adverse events were decreased WBCs (20%), nonfebrile neutropenia (12%), anemia (9%), elevated liver enzymes (4%), and hyperglycemia (11%). 3 patients had a CR, 4 had a PR, and 4 patients had SD > six months. ORR was 47% and CBR was 73% in 15 evaluable patients. Including all 19 enrolled patients, the PFS was 11 months and OS is still ongoing at 17 months. Conclusions: The combination of sapanisertib, serabelisib, and paclitaxel was safe and generally well tolerated. Preliminary efficacy was remarkable in an area of unmet need, especially for patient with PI3K/AKT/mTOR pathway aberrations. Positive effects and sustained clinical benefit were even seen in patients that were refractory to platinum and had failed taxane, everolimus, or temsirolimus. Clinical Trial number : ClinicalTrials.gov , NCT03154294 • Sapanisertib, serabelisib and paclitaxel was a safe and well tolerated combination in heavily pretreated patients • Antitumor activity was observed, especially with endometrioid endometrial cancer and those with PI3K aberrations • In this heavily pretreated population with advanced solid tumors, the ORR = 47%, the CBR = 73%, PFS = 11 months, and OS = 17 months, and is ongoing • The study was awarded a plenary session at this year's Society of Gynecologic Oncology meeting in March 2022 [ABSTRACT FROM AUTHOR]

Progress in understanding physiological mechanisms often consists of discrete discoveries made across different models and species. Accordingly, understanding the mechanistic bases for how altering nitric oxide (NO) bioavailability impacts exercise tolerance (or not) depends on integrating information from cellular energetics and contractile regulation through microvascular/vascular control of O 2 transport and pulmonary gas exchange. This review adopts state-of-the-art concepts including the intramyocyte power grid, the Wagner conflation of perfusive and diffusive O 2 conductances, and the Critical Power/Critical Speed model of exercise tolerance to address how altered NO bioavailability may, or may not, affect physical performance. This question is germane from the elite athlete to the recreational exerciser and particularly the burgeoning heart failure (and other clinical) populations for whom elevating O 2 transport and/or exercise capacity translates directly to improved life quality and reduced morbidity and mortality. The dearth of studies in females is also highlighted, and areas of uncertainty and questions for future research are identified. • We summarize the effects of nitrate(NO 3 −)/nitrite(NO 2 −) supplementation on exercise tolerance in healthy subjects and heart failure patients, starting with the impact of greater nitric oxide (NO) bioavailability on muscle perfusive and diffusive O 2 conductance up to the mechanisms by which nitrate supplementation enhances muscle contractile function • We also explore how the positive effects of elevated intramyocyte NO bioavailability combine with NO-induced cytochrome c oxidase inhibition to better distribute O 2 across the myocyte • Finally, we highlight what little is known regarding sex differences in response to NO 3 − supplementation and identify next-step questions in this vital arena. [ABSTRACT FROM AUTHOR]

The difference in infant health outcomes by maternal opioid use disorder (OUD) status is understudied. We measured the association between maternal OUD during pregnancy and infant mortality and investigated whether this association differs by infant neonatal opioid withdrawal syndrome (NOWS) or maternal receipt of medication for OUD (MOUD) during pregnancy. We sampled 204,543 Medicaid-paid births from Wisconsin, United States (2010–2018). The primary exposure was any maternal OUD during pregnancy. We also stratified this exposure on NOWS diagnosis (no OUD; OUD without NOWS; OUD with NOWS) and on maternal MOUD receipt (no OUD; OUD without MOUD; OUD with <90 consecutive days of MOUD; OUD with 90+ consecutive days of MOUD). Our outcome was infant mortality (death at age <365 days). Demographic-adjusted logistic regressions measured associations with odds ratios (OR) and 95% confidence intervals (CI). Maternal OUD was associated with increased odds of infant mortality (OR 1.43; 95% CI 1.02–2.02). After excluding infants who died <5 days post-birth (i.e., before the clinical presentation of NOWS), regression estimates of infant mortality did not significantly differ by NOWS diagnosis. Likewise, regression estimates did not significantly differ by maternal MOUD receipt in the full sample. Maternal OUD is associated with an elevated risk of infant mortality without evidence of modification by NOWS nor by maternal MOUD treatment. Future research should investigate potential mechanisms linking maternal OUD, NOWS, MOUD treatment, and infant mortality to better inform clinical intervention. • We examined opioid use disorder (OUD) and infant mortality in a large birth cohort. • Maternal OUD in pregnancy may increase the odds of infant mortality. • Association did not significantly vary by neonatal opioid withdrawal syndrome. • Association did not significantly vary by medication for OUD receipt in pregnancy. [ABSTRACT FROM AUTHOR]

We used three years of house finch, Haemorhous mexicanus , song recordings spanning four decades in the introduced eastern range to assess how individual level cultural transmission mechanisms drive population level changes in birdsong. First, we developed an agent-based model (available as a new R package called 'TransmissionBias') that simulates the cultural transmission of house finch song given different parameters related to transmission biases, or biases in social learning that modify the probability of adoption of particular cultural variants. Next, we used approximate Bayesian computation and machine learning to estimate what parameter values likely generated the temporal changes in diversity in our observed data. We found evidence that strong content bias, likely targeted towards syllable complexity, plays a central role in the cultural evolution of house finch song in the New York metropolitan area. Frequency and demonstrator biases appear to be neutral or absent. Additionally, we estimated that house finch song is transmitted with extremely high fidelity. Future studies can use our simulation framework to better understand how cultural transmission and population declines influence song diversity in wild populations. • We analysed house finch songs from three years spanning four decades in New York. • We compared real data against simulations to make inferences about cultural transmission. • Content bias, likely for syllable complexity, drives cultural evolutionary changes. • Frequency and demonstrator biases were absent and transmission fidelity was high. [ABSTRACT FROM AUTHOR]

• A significant proportion of private hospitals experience financial distress • For-profit status was associated with an increased risk of financial distress • A higher share of Medicaid revenue was associated with increased odds of distress Hospitals are closing after poor financial performance leaving many patients without access to medical care. Identifying the factors associated with financial distress offers hospitals avenues for potential intervention to avoid bankruptcy and closure. We performed a retrospective analysis of private U.S. hospitals' financial information from 2011 to 2018. A mixed effects logistic regression model was used with the primary outcome of hospital financial distress (based on the Altman Z-score). Our sample included 2,720 private hospitals contributing a total of 20,022 hospital-year observations. The proportion of hospitals experiencing financial distress each year ranged from 22.0% to 24.3%. For-profit status was associated with an increased odds of financial distress (adjusted odds ratio (aOR), 4.36 [95% Confidence Interval (CI) 3.05 - 6.24]) as compared to non-profit status. A higher share of hospital revenue from Medicaid was also associated with increased odds of financial distress (aOR for the highest quartile, 2.28 [95% CI 1.73 - 3.00]) as compared to the lowest quartile. A higher case mix index (aOR for the highest quartile, 0.32 [95% CI 0.23 - 0.46]) and an increased share of hospital revenue from outpatient services (aOR for the highest quartile, 0.34 [95% CI 0.23 - 0.49]) were associated with decreased odds of financial distress as compared to their respective lowest quartiles. A significant proportion of private U.S. hospitals experience financial distress. Increasing case complexity and the proportion of patient revenue from outpatient services may represent avenues to avoid financial distress. [ABSTRACT FROM AUTHOR]

• 75 adolescents read and heard time-locked narratives during an fMRI scan. • Inter-subject correlation (ISC) was used to analyze reading ability's impact. • ISC was more sensitive to individual differences in reading ability than GLMs. • Reading ability affects brain activity in more regions than previously thought. • Worse readers had more idiosyncratic responses than better readers across the brain. Recent work using fMRI inter-subject correlation analysis has provided new information about the brain's response to video and audio narratives, particularly in frontal regions not typically activated by single words. This approach is very well suited to the study of reading, where narrative is central to natural experience. But since past reading paradigms have primarily presented single words or phrases, the influence of narrative on semantic processing in the brain – and how that influence might change with reading ability – remains largely unexplored. In this study, we presented coherent stories to adolescents and young adults with a wide range of reading abilities. The stories were presented in alternating visual and auditory blocks. We used a dimensional inter-subject correlation analysis to identify regions in which better and worse readers had varying levels of consistency with other readers. This analysis identified a widespread set of brain regions in which activity timecourses were more similar among better readers than among worse readers. These differences were not detected with standard block activation analyses. Worse readers had higher correlation with better readers than with other worse readers, suggesting that the worse readers had "idiosyncratic" responses rather than using a single compensatory mechanism. Close inspection confirmed that these differences were not explained by differences in IQ or motion. These results suggest an expansion of the current view of where and how reading ability is reflected in the brain, and in doing so, they establish inter-subject correlation as a sensitive tool for future studies of reading disorders. [ABSTRACT FROM AUTHOR]

• Cortico-cerebellar functional connectivity predicts cerebellar task activation. • A single connectome measurement can predict across a multi-domain task battery. • Predictions are unique to the individual. • Findings indicate highly specific cerebellar contributions to cognition. The cerebellum is gaining scientific attention as a key neural substrate of cognitive function; however, individual differences in the cerebellar organization have not yet been well studied. Individual differences in functional brain organization can be closely tied to individual differences in brain connectivity. 'Connectome Fingerprinting' is a modeling approach that predicts an individual's brain activity from their connectome. Here, we extend 'Connectome Fingerprinting' (CF) to the cerebellum. We examined functional MRI data from 160 subjects (98 females) of the Human Connectome Project young adult dataset. For each of seven cognitive task paradigms, we constructed CF models from task activation maps and resting-state cortico-cerebellar functional connectomes, using a set of training subjects. For each model, we then predicted task activation in novel individual subjects, using their resting-state functional connectomes. In each cognitive paradigm, the CF models predicted individual subject cerebellar activity patterns with significantly greater precision than did predictions from the group average task activation. Examination of the CF models revealed that the cortico-cerebellar connections that carried the most information were those made with the non-motor portions of the cerebral cortex. These results demonstrate that the fine-scale functional connectivity between the cerebral cortex and cerebellum carries important information about individual differences in cerebellar functional organization. Additionally, CF modeling may be useful in the examination of patients with cerebellar dysfunction, since model predictions require only resting-state fMRI data which is more easily obtained than task fMRI. [ABSTRACT FROM AUTHOR]

It remains an elusive goal to simultaneously model the astrophysics of Solar System accretion while reproducing the mantle chemistry of more than one inner terrestrial planet. Here, we used a multistage core-mantle differentiation model based on Rubie et al. (2011, 2015) to track the formation and composition of Earth and Mars in various Grand Tack formation simulations. Prior studies showed that in order to recreate Earth's mantle composition, it must grow first from reduced (Fe-metal rich and O-poor) building blocks and then from increasingly oxidized (FeO rich) material. This accretion chemistry occurs when an oxidation gradient exists across the disk so that the innermost solids are reduced and increasingly oxidized material is found at greater heliocentric distances. For a suite of Grand Tack simulations, we investigated whether Earth and Mars can be simultaneously produced by the same oxidation gradient. Our model did not find an oxidation gradient that simultaneously reproduces the mantle compositions of Earth and Mars. Due to its small mass and rapid formation, the formation history of Mars-like planets is very stochastic which decreases the likelihood of compatibility with an Earth-producing oxidation gradient in any given realization. To reconcile the accretion history and ideal chemistry of the Mars-like planet with the oxidation gradient of an Earth-producing disk, we determined where in the Earth-producing disk Mars must have formed. We find that the FeO-rich composition of the Martian mantle requires that Mars' building blocks must originate exterior to 1.0 astronomical units (AU). • Earth's building blocks reflect an average of the entire inner disk, unlike Mars. • Mars' growth history varies greatly between simulations due to dynamical mixing. • Mars' building blocks must originate exterior to 1 AU to reproduce FeO rich mantle. [ABSTRACT FROM AUTHOR]

We advance a recent comprehensive model of climate change risk perceptions by investigating the role of media in shaping individuals' health risk perceptions. Results from a national survey in Australia (n = 1023) show that perceived health risks associated with climate change are related but distinct from perceived harm to self and society as measured in previous risk perception studies. Moreover, the full model explains 47% variance in health risks and 74% in perceived harm; While experiential processes (21%) account for about half of the variance in health risks, cognitive factors explain the largest share of variance in perceived harm (32%). Media processes explain a 10% variance in health risks and a 14% variance in perceived harm. News interest and media exposure to extreme weather events are significant to understand public health risks and harm perceptions, even after accounting for several other socio-demographic, cognitive, experiential, and socio-cultural factors. Findings support a conditional media effects model. Media can, directly and indirectly, affect public health risk perceptions by providing vicarious experience opportunities and information that Australians are looking for in the media about climate change. • Media processes explain significant variance in climate change related health risks. • Cognitive factors explain the largest share of variance in perceived harm. • Experiential processes account for about half of the variance in health risks. [ABSTRACT FROM AUTHOR]

[Display omitted] Electronic health records (EHR), containing detailed longitudinal clinical information on a large number of patients and covering broad patient populations, open opportunities for comprehensive predictive modeling of disease progression and treatment response. However, since EHRs were originally constructed for administrative purposes not for research, in the EHR-linked studies, it is often not feasible to capture reliable information for analytical variables, especially in the survival setting, when both accurate event status and event times are needed for model building. For example, progression-free survival (PFS), a commonly used survival outcome for cancer patients, often involves complex information embedded in free-text clinical notes and cannot be extracted reliably. Proxies of PFS time such as time to the first mention of progression in the notes are at best good approximations to the true event time. This leads to difficulty in efficiently estimating event rates for an EHR patient cohort. Estimating survival rates based on error-prone outcome definitions can lead to biased results and hamper the power in the downstream analysis. On the other hand, extracting accurate event time information via manual annotation is time and resource intensive. The objective of this study is to develop a calibrated survival rate estimator using noisy outcomes from EHR data. In this paper, we propose a two-stage semi-supervised calibration of noisy event rate (SCANER) estimator that can effectively overcome censoring induced dependency and attains more robust performance (i.e., not sensitive to misspecification of the imputation model) by fully utilizing both a small-labeled set of gold-standard survival outcomes annotated via manual chart review and a set of proxy features automatically captured via EHR in the unlabeled set. We validate the SCANER estimator by estimating the PFS rates for a virtual cohort of lung cancer patients from one large tertiary care center and the ICU-free survival rates for COVID patients from two large tertiary care centers. In terms of survival rate estimates, the SCANER had very similar point estimates compared to the complete-case Kaplan Meier estimator. On the other hand, other benchmark methods for comparison, which fail to account for the induced dependency between event time and the censoring time conditioning on surrogate outcomes, produced biased results across all three case studies. In terms of standard errors, the SCANER estimator was more efficient than the KM estimator, with up to 50% efficiency gain. The SCANER estimator achieves more efficient, robust, and accurate survival rate estimates compared to existing approaches. This promising new approach can also improve the resolution (i.e., granularity of event time) by using labels conditioning on multiple surrogates, particularly among less common or poorly coded conditions. [ABSTRACT FROM AUTHOR]

Exposure to perfluoroalkyl substances (PFAS) has been associated with changes in insulin sensitivity and pancreatic beta-cell function in humans. Genetic predisposition to diabetes may modify these associations; however, this hypothesis has not been yet studied. To evaluate genetic heterogeneity as a modifier in the PFAS association with insulin sensitivity and pancreatic beta-cell function, using a targeted gene – environment (GxE) approach. We studied 85 single-nucleotide polymorphisms (SNPs) associated with type 2 diabetes, in 665 Faroese adults born in 1986–1987. Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) were measured in cord whole blood at birth and in participants' serum from age 28 years. We calculated the Matsuda-insulin sensitivity index (ISI) and the insulinogenic index (IGI) based on a 2 h-oral glucose tolerance test performed at age 28. Effect modification was evaluated in linear regression models adjusted for cross-product terms (PFAS*SNP) and important covariates. Prenatal and adult PFOS exposures were significantly associated with decreased insulin sensitivity and increased beta-cell function. PFOA associations were in the same direction but attenuated compared to PFOS. A total of 58 SNPs were associated with at least one PFAS exposure variable and/or Matsuda-ISI or IGI in the Faroese population and were subsequently tested as modifiers in the PFAS-clinical outcome associations. Eighteen SNPs showed interaction p-values (P GxE) < 0.05 in at least one PFAS-clinical outcome association, five of which passed False Discovery Rate (FDR) correction (P GxE-FDR <0.20). SNPs for which we found stronger evidence for GxE interactions included ABCA1 rs3890182, FTO rs9939609, FTO rs3751812, PPARG rs170036314 and SLC12A3 rs2289116 and were more clearly shown to modify the PFAS associations with insulin sensitivity, rather than with beta-cell function. Findings from this study suggest that PFAS-associated changes in insulin sensitivity could vary between individuals as a result of genetic predisposition and warrant replication in independent larger populations. • Exposure to perfluoroalkyl substances (PFAS) is ubiquitous and associated with diabetes risk. • We examined targeted single-nucleotide polymorphisms as effect modifiers in this association. • Findings suggest that PFAS associations with insulin sensitivity could be modified by genetic predisposition. • Results warrant replication in larger independent populations. [ABSTRACT FROM AUTHOR]

Clinical guidelines recommend screening people with epilepsy (PWE) regularly for mental distress, but it is unclear how guidelines are implemented. We surveyed epilepsy specialists in adult Scottish services to determine approaches used to screen for anxiety, depression, and suicidality; the perceived difficulty of screening; factors associated with intention to screen; and treatment decisions made following positive screens. An anonymous email-based questionnaire survey of epilepsy nurses and epilepsy neurology specialists (n = 38) was conducted. Two in every three specialists used a systematic screening approach; a third did not. Clinical interview was employed more often than standardized questionnaire. Clinicians reported positive attitudes towards screening but found screening difficult to implement. Intention to screen was associated with favorable attitude, perceived control, and social norm. Pharmacological and non-pharmacological interventions were proposed equally often for those screening positive for anxiety or depression. Routine screening for mental distress is carried out in Scottish epilepsy treatment settings but is not universal. Attention should be paid to clinician factors associated with screening, such as intention to screen and resulting treatment decisions. These factors are potentially modifiable, offering a means of closing the gap between guideline recommendations and clinical practice. [ABSTRACT FROM AUTHOR]

• The mental health burden for people with epilepsy (PWE) is considerable. • Screening for mental distress, though recommended, is often not done routinely. • We report the feasibility of a postal-telephone mental distress screening pathway. • Mood and quality of life (QOL) outcomes improved for PWE included in the pathway. • However, 1 in 3 PWE could not be screened, possibly those struggling most with mood. Mental distress is present in a significant proportion of people with epilepsy (PWE), with a negative impact across life domains. It is underdiagnosed and under-treated despite guidelines recommending screening for its presence (e.g., SIGN, 2015). We describe a tertiary-care epilepsy mental distress screening and treatment pathway, with a preliminary investigation of its feasibility. We selected psychometric screening instruments for depression, anxiety, quality of life (QOL), and suicidality, establishing treatment options matched to instrument scores on the Patient Health Questionnaire 9 (PHQ-9), along 'traffic light' lines. We determined feasibility outcomes including recruitment and retention rates, resources required to run the pathway, and level of psychological need. We undertook a preliminary investigation of change in distress scores over a 9-month interval and determined PWE engagement and the perceived usefulness of pathway treatment options. Two-thirds of eligible PWE were included in the pathway with an 88% retention rate. At the initial screen, 45.8% of PWE required either an 'Amber-2' intervention (for moderate distress) or a 'Red' one (for severe distress). The equivalent figure at the 9-month re-screen was 36.8%, reflective of an improvement in depression and QOL scores. Online charity-delivered well-being sessions and neuropsychology were rated highly for engagement and perceived usefulness, but computerized cognitive behavioral therapy was not. The resources required to run the pathway were modest. Outpatient mental distress screening and intervention are feasible in PWE. The challenge is to optimize methods for screening in busy clinics and to determine the best (and most acceptable) interventions for screening positive PWE. [ABSTRACT FROM AUTHOR]

While hypogammaglobulinemia is associated with COPD exacerbations, it is unknown whether frequent exacerbators have specific defects in antibody production/function. We hypothesized that reduced quantity/function of serum pneumococcal antibodies correlate with exacerbation risk in the SPIROMICS cohort. We measured total pneumococcal IgG in n = 764 previously vaccinated participants with COPD. In a propensity-matched subset of n = 200 with vaccination within five years (n = 50 without exacerbations in the previous year; n = 75 with one, n = 75 with ≥2), we measured pneumococcal IgG for 23 individual serotypes, and pneumococcal antibody function for 4 serotypes. Higher total pneumococcal IgG, serotype-specific IgG (17/23 serotypes), and antibody function (3/4 serotypes) were independently associated with fewer prior exacerbations. Higher pneumococcal IgG (5/23 serotypes) predicted lower exacerbation risk in the following year. Pneumococcal antibodies are inversely associated with exacerbations, supporting the presence of immune defects in frequent exacerbators. With further study, pneumococcal antibodies may be useful biomarkers for immune dysfunction in COPD. [ABSTRACT FROM AUTHOR]

• Biological control of Sirex noctilio in North America with Deladenus is explored. • The woodwasp's symbiotic fungus can impact nematode parasitism. • Amylostereum fungus species and strain affected nematode reproduction. • Mycophagous Deladenus juveniles never avoided non-host fungi. • Deladenus proximus could produce some eggs on non-host fungi. Dimorphic nematodes in the genus Deladenus have been used or are being considered for use in biological control of the invasive Eurasian woodwasp, Sirex noctilio , which threatens pine (Pinus spp.) trees. Deladenus species that are parasitic on Sirex can kill woodwasp eggs and occupy these same eggs for their own dispersal. These nematodes also have mycophagous phases that feed on the white rot fungal symbionts of Sirex , Amylostereum species. The mycophagous stage of the Hungarian strain of Deladenus siricidicola developed for control of S. noctilio in Australia feeds exclusively on A. areolatum. The mycophagous stage of a North American Deladenus species being evaluated for biological control, D. proximus , feeds on either A. chailletii, or A. areolatum. Amylostereum species and strains associated with Sirex have differential impacts on survival and growth of these nematodes. We investigated whether differences in species and strains of Amylostereum influence the numbers of Deladenus juveniles adjacent to cultures, as this would impact potential parasitism of Sirex. Fungal species or strain did not influence persistence of juveniles in the fungal vicinity although retention could be influenced by the fungal strain consumed by parents. Investigating D. proximus , we tested whether the most common invasive strain of A. areolatum associated with S. noctilio in North America (IGS D) impacted nematode growth, compared with the common native Amylostereum chailletii. Deladenus proximus increased very slowly when feeding on A. areolatum IGS D, compared with A. chailletii ; when provided A. areolatum IGS D, 55 eggs were produced after 4 weeks compared with 8.1 × 104 eggs after 2 weeks when A. chailletii was provided. In summary, behavior of Deladenus juveniles resulted in no or low avoidance of Amylostereum species and strains, regardless of poor growth when mycophagous forms fed on fungi on which growth was poor.. [ABSTRACT FROM AUTHOR]

Imidacloprid is a neonicotinoid neurotoxin that remains widely used worldwide and persists in the environment, resulting in chronic exposure to non-target insects. To accurately map dose-dependent effects of such exposure across taxa, toxicological assays need to assess relevant modes of exposure across indicator species. However, due to the difficulty of these experiments, contact bioassays are most frequently used to quantify dose. Here, we developed a novel naturalistic feeding bioassay to precisely measure imidacloprid ingestion and its toxicity for acute and chronic exposure in a dipteran, Eristalis tenax L., an important member of an under-represented pollinator group. Flies which ingested imidacloprid dosages lower than 12.1 ng/mg all showed consistent intake volumes and learned improved feeding efficiency over successive feeding sessions. In contrast, at doses of 12.1 ng/mg and higher flies showed a rapid onset of severe locomotive impairment which prevented them from completing the feeding task. Neither probability of survival nor severe locomotive impairment were significantly higher than the control group until doses of 1.43 ng/mg or higher were reached. We were unable to measure a median lethal dose for acute exposure (72 h) due to flies possessing a relatively high tolerance for imidacloprid. However, with chronic exposure (18 days), mortality went up and an LD50 of 0.41 ng/mg was estimated. Severe locomotive impairment (immobilisation) tended to occur earlier and at lower dosages than lethality, with ED50s of 7.82 ng/mg and 0.17 ng/mg for acute and chronic exposure, respectively. We conclude that adult Eristalis possess a much higher tolerance to this toxin than the honeybees that they mimic. The similarity of the LD50 to other dipterans such as the fruitfly and the housefly suggests that there may be a phylogenetic component to pesticide tolerance that merits further investigation. The absence of obvious adverse effects at sublethal dosages also underscores a need to develop better tools for quantifying animal behaviour to evaluate the impact of insecticides on foraging efficiency in economically important species. [Display omitted] • Relevant modes of pesticide exposure need to be studied across more varied taxa. • We used a naturalistic system to control neonicotinoid intake in a dipteran pollinator. • Flies learned to feed more efficiently and consumed consistent volumes over time. • We determined the lethal dose for both acute (72 h) and chronic (18d) exposure. • Eristalis is 2–3 orders of magnitude more tolerant to imidacloprid than honeybees. [ABSTRACT FROM AUTHOR]

Prenatal exposure to elements may be associated with birth weight via shortening of gestation. This study aimed to determine if prenatal exposure is associated with birth weight, and to explore the potential mediating role of gestational age in the association. Within an established Bangladesh prospective birth cohort (2008–2011), we analyzed the concentrations of 15 elements in maternal serum samples collected during the first (n = 780) and second (n = 610) trimesters using inductively coupled plasma mass spectrometry. Mediation analyses explored the relationships between these elements, gestational age, and birth weight. Serum concentrations of cobalt (Co) (first trimester: b = 56.5; 95% confidence interval [CI]: 13.5–99.0; false discovery rate [FDR] -q = 0.035; second trimester: b = 73.3; 95% CI: 20.4–130.2; FDR -q = 0.037) and antimony (Sb) in both trimesters (first trimester: b = 92.1; 95% CI: 66.0–118.9; FDR - q < 0.001; second trimester: b = 93.3; 95% CI: 67.3–118.4; FDR -q < 0.001), and strontium (Sr) in the first trimester (b = 142.4; 95% CI: 41.6–247.9; FDR -q = 0.035) were positively associated with birth weight, while negative associations were observed for barium (Ba) (first trimester: b = −154.8; 95% CI: −217.9 to 91.8; FDR -q <0.001; second trimester: b = −26.7; 95% CI: −44.9 to 10.2; FDR -q < 0.001). These elements act partially by affecting gestation age and appear to have heightened impact among smaller infants. Further research is needed to determine the biological underpinnings of these effects, which may inform strategies to avert low birth weight. • Co, Sr, and Sb may be beneficial as nutritional supplements for pregnant women. • Ba exerted adverse effects on fetal development. • Co, Sr, and Ba acted partially by affecting gestation age. • Associations between Co, Sr, and Ba and birthweight varied across percentiles. [ABSTRACT FROM AUTHOR]

• New class of provably stable flux reconstruction schemes for curvilinear elements. • Conservative and non-conservative schemes inherently different on curvilinear grids. • Flux Reconstruction correction functions must include metric dependence. • Flux reconstruction in split form only reconstructing surface is divergent. • Provable stability only if correction functions reconstruct the volume and surface. Provably stable flux reconstruction (FR) schemes are derived for partial differential equations cast in curvilinear coordinates. Specifically, energy stable flux reconstruction (ESFR) schemes are considered as they allow for design flexibility as well as stability proofs for the linear advection problem on affine elements. Additionally, the curvilinear metric split-form for a linear physical flux is examined as it enables the development of energy stability proofs. The first critical step proves, that in curvilinear coordinates, the discontinuous Galerkin (DG) conservative and non-conservative forms are inherently different–even under exact integration and analytically exact metric terms. This analysis demonstrates that the split form is essential to developing provably stable DG schemes on curvilinear coordinates and motivates the construction of metric dependent ESFR correction functions in each element. Furthermore, the provably stable FR schemes differ from schemes in the literature that only apply the ESFR correction functions to surface terms or on the conservative form, and instead incorporate the ESFR correction functions on the full split form of the equations. It is demonstrated that the scheme is divergent when the correction functions are only used for surface reconstruction in curvilinear coordinates. We numerically verify the stability claims for our proposed FR split forms and compare them to ESFR schemes in the literature. Lastly, the newly proposed provably stable FR schemes are shown to obtain optimal orders of convergence. The scheme loses the orders of accuracy at the equivalent correction parameter value c as that of the one-dimensional ESFR scheme. [ABSTRACT FROM AUTHOR]

Behavioral plasticity refers to changes occurring due to external influences on an organism, including adaptation, learning, memory and enduring influences from early life experience. There are 2 types of behavioral plasticity: "developmental", which refers to gene/environment interactions affecting a phenotype, and "activational" which refers to innate physiology and can involve structural physiological changes of the body. In this review, we focus on feeding behavior, and studies involving neuropeptides that influence behavioral plasticity - primarily opioids, orexin, neuropeptide Y, and oxytocin. In each section of the review, we include examples of behavioral plasticity as it relates to actions of these neuropeptides. It can be concluded from this review that eating behavior is influenced by a number of external factors, including time of day, type of food available, energy balance state, and stressors. The reviewed work underscores that environmental factors play a critical role in feeding behavior and energy balance, but changes in eating behavior also result from a multitude of non-environmental factors, such that there can be no single mechanism or variable that can explain ingestive behavior. [ABSTRACT FROM AUTHOR]

When exposed to the same sensory event, some individuals are bound to have less typical experiences than others. Previous research has investigated this phenomenon by showing that the typicality of one's sensory experience is associated with the typicality of their stimulus-evoked brain activity (as measured by intersubject correlation, or ISC). Individual differences in ISC have recently been attributed to variability in focal neural processing. However, the extent to which these differences reflect purely intra-regional variability versus variation in the brain's baseline ability to transmit information between regions has yet to be established. Here, we show that an individual's degree and spatial distribution of ISC are closely related to their brain's functional organization at rest. Using resting state and movie watching fMRI data from the Human Connectome Project, we reveal that resting state functional connectivity (RSFC) profiles can be used to predict cortex-wide ISC. Similar region-level analyses demonstrate that the levels of ISC exhibited by brain regions during movie watching are associated with their connectivity to other regions at rest, and that the nature of these connectivity-activity relationships varies as a function of regional roles in sensory information processing. Finally, we show that an individual's unique spatial distribution of ISC, independent of its magnitude, is also related to their RSFC profile. These findings contextualize reports of localized individual differences in ISC as potentially reflecting larger, network-level alterations in resting brain function and detail how the brain's ability to process complex sensory information is linked to its baseline functional organization. [ABSTRACT FROM AUTHOR]

The objective of this study was to characterize vitellogenin (VTG) protein in male fathead minnow (Pimephales promelas) mucus compared with more conventional measures in plasma and mRNA isolated from liver. To assess the intensity and duration of changes in mucus VTG concentrations, male fathead minnows were exposed to 17α-ethinylestradiol (EE2) for 7 days with a subsequent depuration period of 14 days. The experiment was conducted in a flow-through system to maintain a consistent concentration of EE2 at a nominal EC 50 concentration of 2.5 ng/L and high concentration of 10 ng/L as a positive control. Mucus, plasma and liver were sampled at regular intervals throughout the study. Relative abundance of vtg mRNA increased after 2 days of exposure and returned to control levels after 4 days of depuration. VTG protein concentration displayed similar induction kinetics in both mucus and plasma, however, it was found to be significantly increased after 2 days of exposure using the mucus-based assays and 7 days with the plasma-based assay. Significantly elevated levels of VTG were detected by both assays throughout the 14-day depuration period. The elimination of the laborious plasma collection step in the mucus-based workflow allowed sampling of smaller organisms where blood volume is limiting. It also resulted in significant gains in workflow efficiency, decreasing sampling time without loss of performance. • Vitellogenin was detected in fathead minnow mucus with a commercial ELISA kit. • Vitellogenin mRNA expression in liver and protein in mucus can time exposure. • Vitellogenin in mucus was comparable to plasma levels. [ABSTRACT FROM AUTHOR]

Diffusion MRI measures of the human brain provide key insight into microstructural variations across individuals and into the impact of central nervous system diseases and disorders. One approach to extract information from diffusion signals has been to use biologically relevant analytical models to link millimetre scale diffusion MRI measures with microscale influences. The other approach has been to represent diffusion as an anomalous transport process and infer microstructural information from the different anomalous diffusion equation parameters. In this study, we investigated how parameters of various anomalous diffusion models vary with age in the human brain white matter, particularly focusing on the corpus callosum. We first unified several established anomalous diffusion models (the super-diffusion, sub-diffusion, quasi-diffusion and fractional Bloch-Torrey models) under the continuous time random walk modelling framework. This unification allows a consistent parameter fitting strategy to be applied from which meaningful model parameter comparisons can be made. We then provided a novel way to derive the diffusional kurtosis imaging (DKI) model, which is shown to be a degree two approximation of the sub-diffusion model. This link between the DKI and sub-diffusion models led to a new robust technique for generating maps of kurtosis and diffusivity using the sub-diffusion parameters β S U B and D S U B. Superior tissue contrast is achieved in kurtosis maps based on the sub-diffusion model. 7T diffusion weighted MRI data for 65 healthy participants in the age range 19–78 years was used in this study. Results revealed that anomalous diffusion model parameters α and β have shown consistent positive correlation with age in the corpus callosum, indicating α and β are sensitive to tissue microstructural changes in ageing. [ABSTRACT FROM AUTHOR]

Working memory is the ability to keep information in one's mind and mentally manipulate it. Decrements in working memory play a key role in many behavioral and psychiatric disorders, therefore identifying modifiable environmental risk factors for such decrements is important for mitigating these disorders. There is some evidence that prenatal exposure to individual chemicals may adversely impact working memory among children, but few studies have explored the association of co-exposure to multiple chemicals with this outcome in adolescence, a time when working memory skills undergo substantial development. We investigated the association of organochlorines (DDE, HCB, PCBs) and metals (lead, manganese) measured in cord serum and cord blood, respectively, with working memory measured with the Wide Range Assessment of Memory and Learning, 2nd Edition among 373 adolescents living near a Superfund site in New Bedford, Massachusetts. We used Bayesian Kernel Machine Regression (BKMR) and linear regression analyses and assessed effect modification by sex and prenatal social disadvantage. In BKMR models, we observed an adverse joint association of the chemical mixture with Verbal, but not Symbolic, Working Memory. In co-exposure and covariate-adjusted linear regression models, a twofold increase in cord blood manganese was associated with lower working memory scaled scores, with a stronger association with Verbal Working Memory (difference = −0.75; 95% CI: −1.29, −0.20 points) compared to Symbolic Working Memory (difference = −0.44; 95% CI: −1.00, 0.12 points). There was little evidence of effect modification by sex and some evidence associating organochlorine pesticides with poorer working memory scores among those with greater prenatal social disadvantage. This study provided evidence of an adverse joint association of a chemical mixture with a verbal working memory task among adolescents, as well as an adverse association of prenatal manganese exposure with working memory. • Prenatal chemical mixture associated with poorer working memory in adolescence. • Strongest associations were for prenatal manganese and verbal working memory. • DDE associated with poorer working memory among those with social disadvantage. • Prenatal exposures can impact key aspects of adolescent cognitive development. [ABSTRACT FROM AUTHOR]

[Display omitted] We analyze dynamic adsorption of surfactant from a micellar solution to a rapidly created surface that acts as an absorbing boundary for surfactant monomers (single molecules), along which the monomer concentration vanishes, with no direct micelle adsorption. This somewhat idealized situation is analyzed as a prototype for situations in which strong suppression of monomer concentration accelerates micelle dissociation, and will be used as a starting point for analysis of more realistic boundary conditions in subsequent work. We present scaling arguments and approximate models for particular time and parameter regimes and compare the resulting predictions to numerical simulations of the reaction–diffusion equations for a polydisperse system containing surfactant monomers and clusters of arbitrary aggregation number. The model considered here exhibits an initial period of rapid shrinkage and ultimate dissociation of micelles within a narrow region near the interface. This opens a micelle-free region near the interface after some time τ e , the width of which increases as t 1 / 2 at times t ≫ τ e . In systems that exhibit disparate fast and slow bulk relaxation times τ 1 and τ 2 in response to small perturbations, τ e is usually comparable to or greater than τ 1 but much less than τ 2 . Such systems exhibit a wide intermediate time regime τ e < t < τ 2 in which the remaining micellar region reaches a state of partial local equilibrium, followed by a final stage t ≫ τ 2 in which full local equilibrium is established. [ABSTRACT FROM AUTHOR]

• Mathematical competencies and self-evaluations are placed in evolutionary context. • Evolved brain and cognitive systems that support mathematical learning are discussed. • Sex differences in evolved systems and mathematics learning are discussed. • Historical, cultural, and experiential influences on this learning are also discussed. • Mathematics attitudes are considered in the context of the evolution of self-awareness. The articles in this special issue provide state-of-the-art reviews of the brain and cognitive systems that are engaged during some aspects of mathematical learning, as well as the self-beliefs, anxiety, and social factors that influence engagement with mathematics, along with discussion of any associated sex differences. These issues are integrated into an evolutionary perspective that includes discussion of how evolved brain and cognitive systems might be co-opted for learning in the evolutionarily novel domain of mathematics. Attitudes and beliefs about mathematics are considered in the context of the evolution of self-awareness that in turn explains why many students do not value mathematics, despites its importance in the modern world, as highly as many other personal traits, such as their physical appearance. The overall argument is that reflecting on academic learning and attitudes from an evolutionary perspective provides insights into student learning and self-beliefs about learning that might otherwise elude explanation. [ABSTRACT FROM AUTHOR]

The literature on gender homophily has mostly been focused on patient-physician relationship but not on interprofessional referrals. The goal of this study is to quantify interphysician gender homophily of referring physicians in surgical referrals. An observational study of the referral data at a large academic center was performed. Patients referred through Epic to the department of general surgery from January 2016 to October 2019 were included. The primary end point was gender homophily and the primary independent variable was referring physician gender. Gender homophily was defined as greater than expected rates of gender concordance. Gender concordance was defined when referring physicians have the same gender as receiving surgeons. The expected concordance rate was defined as the availability of gender-concordant surgeons in the population. Absolute homophily is the difference between observed and expected concordance rates, whereas relative homophily is the ratio between observed and expected concordance rates. A total of 25,271 patient referrals from 2625 referring physicians to 91 surgeons were analyzed. The overall observed concordance rate for the entire study population was 55.3% and was 31.7% among female physicians and 82.4% among male physicians. Compared to the expected concordance rate, the absolute gender homophily among all female physicians was +7.2% or a relative homophily of 1.29%. In contrast, the absolute gender homophily among all male physicians was +6.9% or a relative homophily of 1.09%. Gender homophily exists in interprofessional referrals. Although referral decisions are presumably based solely on clinical factors, referrals can be affected by subjective biases. [ABSTRACT FROM AUTHOR]

• In-class attentive behavior is an important predictor of mathematics achievement. • Adolescent boys are less attentive in classroom settings than girls (d = −.34). • Controlling for attentive behavior, boys have advantages in mathematics achievement. • The sex difference in mathematics achievement is fully mediated by spatial abilities. The mathematics and reading achievement of 322 adolescents (159 boys) was assessed in seventh and eighth grades, as were their intelligence, working memory, and spatial abilities. Their seventh- and eighth-grade mathematics and English language arts teachers reported on their in-class attentive behavior. The latter emerged as an important predictor of achievement, but more so for mathematics than for reading. Boy were less attentive in classroom settings than girls (d = −.34) and performed better than expected in mathematics given their level of engagement in the classroom. Boys' better-than-expected mathematics achievement was related to advantages on visuospatial measures (d s =.28–.56), which fully mediated a sex difference in mathematics (d s =.27–.28) but not in reading achievement, with control of in-class attentive behavior. The results suggest that boys' advantages in visuospatial skills compensate for lower levels of classroom engagement in the learning of mathematics but not in reading competencies. [ABSTRACT FROM AUTHOR]

Ancient DNA analyses have shown that interbreeding between hominin taxa occurred multiple times. Although admixture is often reflected in skeletal phenotype, the relationship between the two remains poorly understood, hampering interpretation of the hominin fossil record. Direct study of this relationship is often impossible due to the paucity of hominin fossils and difficulties retrieving ancient genetic material. Here, we use a sample of known ancestry hybrids between two closely related nonhuman primate taxa (Indian and Chinese Macaca mulatta) to investigate the effect of admixture on skeletal morphology. We focus on pelvic shape, which has potential fitness implications in hybrids, as mismatches between maternal pelvic and fetal cranial morphology are often fatal to mother and offspring. As the pelvis is also one of the skeletal regions that differs most between Homo sapiens and Neanderthals, investigating the pelvic consequences of interbreeding could be informative regarding the viability of their hybrids. We find that the effect of admixture in M. mulatta is small and proportional to the relatively small morphological difference between the parent taxa. Sexual dimorphism appears to be the main determinant of pelvic shape in M. mulatta. The lack of difference in pelvic shape between Chinese and Indian M. mulatta is in contrast to that between Neanderthals and H. sapiens , despite a similar split time (in generations) between the hybridizing pairs. Greater phenotypic divergence between hominins may relate to adaptations to disparate environments but may also highlight how the unique degree of cultural buffering in hominins allowed for greater neutral divergence. In contrast to some previous work identifying extreme morphologies in first- and second-generation hybrids, here the relationship between pelvic shape and admixture is linear. This linearity may be because most sampled animals have a multigenerational admixture history or because of relatively high constraints on the pelvis compared with other skeletal regions. [ABSTRACT FROM AUTHOR]

The nuclear region of the lens is metabolically quiescent, but it is far from inert chemically. Without cellular renewal and with decades of environmental exposures, the lens proteome, lipidome, and metabolome change. The lens crystallins have evolved exquisite mechanisms for resisting, slowing, adapting to, and perhaps even harnessing the effects of these cumulative chemical modifications to minimize the amount of light-scattering aggregation in the lens over a lifetime. Redox chemistry is a major factor in these damages and mitigating adaptations, and as such, it is likely to be a key component of any successful therapeutic strategy for preserving or rescuing lens transparency, and perhaps flexibility, during aging. Protein redox chemistry is typically mediated by Cys residues. This review will therefore focus primarily on the Cys-rich γ-crystallins of the human lens, taking care to extend these findings to the β- and α-crystallins where pertinent. • As the lens loses glutathione, abundant lens crystallins become a redox buffer. • Early crystallin disulfides are compatible with native structure but strained. • With age, disulfides flow from native to misfolded conformers that aggregate. • Transition metals and PTMs alter crystallin redox chemistry, stability, and aggregation. • Creative approaches are needed to repair or preserve lens redox homeostasis. [ABSTRACT FROM AUTHOR]

Soils in urban and built areas are growing in extent as world populations increase, and they provide essential ecological services to meet the complex needs of urban populations. Soils impacted by and formed from human-altered and human-transported (HAHT) materials are spatially heterogeneous, and their properties are typically discontinuous across short distances. Similar to non-urban soils, they inherit properties from the origin of their parent material. Although there is substantial spatial heterogeneity of soil properties, there are common properties such as presence of human artifacts, elevated pH, and greater bulk densities in urban soils compared to reference sites. Each city also has unique soil patterns and characteristics related to a city's native soil composition, cultural and development history, and management practices compared to other cities. Soil profile development has been observed in young HAHT soils and is usually concentrated near the surface layer or upper part of the soil profile. Methods, datasets, and data availability of soil survey maps in urban areas of the United States have progressed considerably, and modern soil surveys have been completed in several major metropolitan areas including New York City, NY, Chicago, IL, Detroit, MI, and Los Angeles, CA. Due to the wealth of new information and interest in urban soils, a new soil order, Artesols, has been proposed to represent soils formed in HAHT materials with significant alteration to soil properties. The new soil order will improve the ability of soil scientists to communicate the diverse soil properties encountered in urban and built areas. [ABSTRACT FROM AUTHOR]

Periprocedural anxiety is common in pediatric patients and is characterized by tension, anxiety, irritability, and autonomic activation. Periprocedural anxiety increases during certain events including admission to the preoperative area, separation from caregivers, induction of anesthesia, and IV placement. A study of children aged 2-12 showed that perioperative anxiety in children may be influenced by high parental anxiety and low sociability of the child. While these are nonmodifiable variables in the perioperative setting, there are numerous ways to ameliorate both parental and patient anxiety including the use of certified child life specialists (CCLSs) to aid in child comfort. In this study, our objective was to evaluate the integration of CCLS in our perioperative setting on the rate of benzodiazepine use. We used a prospectively maintained database to identify patients undergoing outpatient elective surgical and radiologic procedures from July 2022 to September 2023 and January 2023 to September 2023 respectively. CCLSs were used to work with appropriately aged children in order to decrease the use of benzodiazepines and reduce possible adverse events associated with their use. A total of 2175 pediatric patients were seen by CCLS in same day surgery from July 2022 to September 2023. During this period, midazolam use decreased by an average of 11.4% (range 6.2%-19.3%). An even greater effect was seen in the radiologic group with 73% reduction. No adverse events were reported during this period. CCLSs working with age-appropriate patients in the periprocedural setting is a useful adjunct in easing anxiety in pediatric patients, reducing the need for periprocedural benzodiazepine administration and the risk of exposure to unintended side effects. [ABSTRACT FROM AUTHOR]

We report a simple and effective means to increase the biosynthetic capacity of host CHO cells. Lonza proprietary CHOK1SV® cells were evolved by serial sub-culture for over 150 generations at 32 °C. During this period the specific proliferation rate of hypothermic cells gradually recovered to become comparable to that of cells routinely maintained at 37 °C. Cold-adapted cell populations exhibited (1) a significantly increased volume and biomass content (exemplified by total RNA and protein), (2) increased mitochondrial function, (3) an increased antioxidant capacity, (4) altered central metabolism, (5) increased transient and stable productivity of a model IgG4 monoclonal antibody and Fc-fusion protein, and (6) unaffected recombinant protein N-glycan processing. This phenotypic transformation was associated with significant genome-scale changes in both karyotype and the relative abundance of thousands of cellular mRNAs across numerous functional groups. Taken together, these observations provide evidence of coordinated cellular adaptations to sub-physiological temperature. These data reveal the extreme genomic/functional plasticity of CHO cells, and that directed evolution is a viable genome-scale cell engineering strategy that can be exploited to create host cells with an increased cellular capacity for recombinant protein production. • Long-term adaptive evolution of mammalian (CHO) cells to hypothermic growth. • Increased biomass synthesis and recombinant protein production by cold-adapted cells. • Cellular adaptation (karyotypic, metabolic, bioenergetic, transcriptomic) was extensive and conserved. [ABSTRACT FROM AUTHOR]

Previous studies showed that there is variation in ontogenetic trajectories of human limb dimensions and proportions. However, little is known about the evolutionary significance of this variation. This study used a global sample of modern human immature long bone measurements and a multivariate linear mixed-effects model to study 1) whether the variation in ontogenetic trajectories of limb dimensions is consistent with ecogeographic predictions and 2) the effects of different evolutionary forces on the variation in ontogenetic trajectories. We found that genetic relatedness arising from neutral (nonselective) evolution, allometric variation associated with the change in size, and directional effects from climate all contributed to the variation in ontogenetic trajectories of all major long bone dimensions in modern humans. After accounting for the effects of neutral evolution and holding other effects considered in the current study constant, extreme temperatures have weak, positive associations with diaphyseal length and breadth measurements, while mean temperature shows negative associations with diaphyseal dimensions. The association with extreme temperatures fits the expectations of ecogeographic rules, while the association with mean temperature may explain the observed among-group variation in intralimb indices. The association with climate is present throughout ontogeny, suggesting an explanation of adaptation by natural selection as the most likely cause. On the other hand, genetic relatedness among groups, as structured by neutral evolutionary factors, is an important consideration when interpreting skeletal morphology, even for nonadult individuals. [ABSTRACT FROM AUTHOR]

Theory in evolutionary educational psychology (EEP) distinguishes between evolved or biologically primary knowledge and non-evolved or biologically secondary knowledge that emerges with formal schooling. The current study explores the associated argument that framing biologically secondary mathematics learning in biologically primary contexts will increase students' learning motivation. We investigated this hypothesis by presenting standard math content in primary scenarios to a sample of Grade 9 adolescents (n = 32, age = 15) and compared their motivation before and after the intervention. Quantitative results showed an increase in the students' motivation scores from pre-to-post intervention comparisons, and qualitative interviews confirmed their positive attitudes toward learning mathematics. The results are discussed from an evolutionary point of view, and the theory's implications for improving classrooms' environments are outlined. • The students' motivation in an evolutionary-based learning condition has been examined. • The evolutionary-based learning condition positively affected the students' motivation. • The students' enjoyment of learning mathematics increased significantly. • The students' concentration on mathematical problems increased significantly. • The students' anxiety on mathematical tests decreased significantly. [ABSTRACT FROM AUTHOR]

Operations in copper sulfide flotation plants (CSFP) are complex and governed by several variables such as available technologies, reagents, and environmental conditions. However, few investigations are related to studying the microbial communities. These aspects provide a reason to compare the bacterial communities of two CSFP operated with freshwater (FwFlo) and seawater (SwFlo), and study whether indigenous bacteria could be used as pyrite bioreagents. Analyses were determined through next-generation sequencing by Illumina MiSeq System and conducted throughout the entire process: (i) minerals before and after grinding; (ii) final concentrate and concentrate thickener overflow; (iii) final tailings and tailings thickener overflow; and (iv) intake water. Bacterial strains from both plants were tested as potential bioreagents, given their tendency to adhere to pyrite after 5 min. In both CSFP, Proteobacteria (relative abundance from 45.48% to 79.22%), followed by Bacteroidetes (9.37%–44.7%), were the most abundant phyla. Regarding species, Algoriphagus olei (11.35%–43.52%) was present exclusively in FwFlo samples in contact with process water and absent in the mineral before grinding, where Cupriavidus metallidurans (16.05%) and Pseudomonas_uc (11.79%) predominated. In SwFlo samples, Marinobacter flavimaris (3.47%–41.1%), and GU061212-s (10.92%–27.63%), were the most abundant microorganisms. All of them were also detected in intake seawater. The strains with the highest adhesion rate (from 29.84% ± 0.14–100%) were phylogenetically identified as species of the genera Marinobacter , Pseudomonas , Idiomarina , Halomonas , Bacillus , Aerocuccus, and Peribacillus. Our results reveal that bacterial communities are critically dependent on process waters during mining activities, and our data depicted that indigenous bacteria could be used as potential pyrite bioreagents, evidenced by a high adhesion rate. It is thus possible to propose that different indigenous bacterial strains could be considered as new bioreagents to reduce the impact of conventional flotation reagents on health from an environment friendly perspective. [Display omitted] • Novel insights into bacterial communities from CSFP operated with freshwater and seawater. • Proteobacteria (79.22%) and Bacteroidetes (44.7%) were high relative abundance phyla in CSFP. • The process water contributes with the high diversity of bacterial species to CSFP. • Isolation of indigenous CSFP bacteria as potential pyrite bioreagents in flotation. [ABSTRACT FROM AUTHOR]

Hemorrhoid disease is very common problem in the Medicare population. Prior work has shown significant variation in county-level practices of hemorrhoidectomy; however, regional variation of rubber band ligation (RBL) has yet to be assessed. This is important as many different practitioners from different specialties can perform this procedure repeatedly in an office-based setting. We aim to evaluate the variation of RBL and hemorrhoidectomy over a 7-y period. Using Medicare part B claims data, we identified all beneficiaries >65 y seen for hemorrhoid disease between 2006 and 2013. Current Procedural Terminology (CPT) codes were used to identify all events for hemorrhoidectomy (46083, 46250, 46255, 46257, 46260, and 46261) or RBL (46221) by hospital referral region (HRR). We determined HRR-level rates of hemorrhoidectomy and RBL per 1000 beneficiaries adjusted for age, sex, and race. We calculated annual coefficients of variation (SD × 100/mean) for hemorrhoidectomy and RBL. 1.2 to 1.3 million fee-for-service Medicare beneficiaries were seen annually for evaluation of hemorrhoid disease. Mean-adjusted annual rates for hemorrhoidectomy by HRRs varied from 4.34 to 63.03 per 1000 beneficiaries. Mean-adjusted rates of RBL by HRRs varied from 7.06 to 163 per 1000 beneficiaries. Annual procedural coefficients of variation over the study period were 41-48 (high) for hemorrhoidectomy and 69-74 (very high) for RBL. While continued high variation exists for hemorrhoidectomy, there is very high variation for RBL between HRRs in treating hemorrhoid disease among Medicare beneficiaries. There are substantial Medicare expenditures in this high-volume population that are likely unwarranted. [ABSTRACT FROM AUTHOR]

An ischemic stroke (IS) is caused due to the lack of blood flow to cerebral tissue. Most of the studies have focused on how stroke affects the localized tissue, but it has been observed that a stroke can cause secondary complications in distant organs, such as Bone Marrow (BM). Our study focused on the effect of ischemic strokes on the bone marrow microenvironment. Bone marrow (BM) is a vital organ that maintains inflammatory homeostasis and aids in the repair of damaged tissue after injury/IS. We used the middle cerebral artery occlusion (MCAO) model of ischemic stroke on adult mice (6 months) and investigated the changes in the BM environment. BM cells were used for western blot and RT-PCR, and the BM supernatant was used for cytokine analysis and extracellular vesicle (EVs) isolation. We observed a significant increase in the total cell number within the BM and an increase in TNF-alpha and MCP-1, which are known for inducing a pro-inflammatory environment. Western blots analysis on the whole BM cell lysate demonstrated elevated levels of inflammatory factors (IL-6, TNF-alpha, and TLR-4) and senescence markers (p21 p16). EVs isolated from the BM supernatant showed no change in size or concentration; however, we found that the EVs carried increased miRNA-141-3p and miRNA-34a. Proteomic analysis on BM-derived EVs showed an alteration in the protein cargo of IS. We observed an increase in FgB, C3, Fn1, and Tra2b levels. The signaling pathway analysis showed mitochondrial function is most affected within the bone marrow. Our study demonstrated that IS induces changes in the BM environment and EVs secreted in the BM. • Ischemic stroke (IS) increases total cell number, elevates pro-inflammatory and senescence markers in the Bone Marrow (BM). • IS alter the BM-Extracellular vesicles (EVs) miRNA-141-3p and miRNA-34a content. • Proteomic analysis showed IS alter the BM-EVs protein cargo FgB, C3, Fn1, and Tra2b. • Overall, IS induces significant alterations in the BM microenvironment. [ABSTRACT FROM AUTHOR]

The CRISM instrument on MRO collected visible-infrared hyperspectral mapping data (HSP; 180 m/pixel, 262 spectral channels covering 365 to 3937 nm) that covered ∼39% of Mars. Here we present results from a new processing pipeline for these data that produces 5° x 5° hyperspectral mapping tiled mosaics that overlap the coverage of recently released multispectral map tile products (version 4 Multispectral Reduced Data Records, MRDRs; 180 m/pixel, 72 spectral channels). These data enable regional investigations into compositional variations that require high spectral resolution or wavelengths not included in MRDRs. In addition to standard processing techniques available in the CRISM Analysis Toolkit (CAT), the pipeline includes a new correction for systematic discrepancies in radiometric calibration between CRISM observing modes, an improved filtering algorithm to remediate noise, and a technique to correct for differences in radiometry among data strips that arise from differences in photometric and atmospheric conditions. Demonstration hyperspectral mapping tiles covering the Mawrth Vallis region were developed and compared with MRDRs and high spatial resolution hyperspectral targeted observations. The new processing pipeline shows an improvement in data quality over standard processing using CAT utilities. Compared to targeted observations (18 or 36 m/pixel, 545 spectral channels), hyperspectral mapping tile mosaics reveal compositional information across a much greater spatial extent at the expense of 5–10 times coarser spatial resolution. In addition, the hyperspectral mapping tile mosaics reveal greater compositional detail in both the spatial and spectral dimensions compared to MRDRs, but with sparser spatial coverage. Sample hyperspectral mapping tile mosaics over the Mawrth Vallis region reveal hydrated silica, Al-bearing smectite, Fe/Mg-bearing smectite, and mixed clays, consistent with compositions previously reported in the literature. • We present new 5° x 5° hyperspectral mapping tiled mosaics comprised ofCRISM hyperspectral mapping data (HSP; 180 m/pixel, 262 spectral channels). • HSP tile mosaics enable regional investigations into compositional variations that require high spectral resolution. • HSP tile mosaics reveal compositional information across a much greater spatial extant than targeted data and with more spatial and spectral detail than MRDR tiles. • For regional to global-scale investigations, HSP tile mosaics could augment the use of MRDRs by adding critical spectral detail. • For local-scale investigations using CRISM targeted observations, HSP mapping data could add contextual information complementary to that which is available with the MRDRs. [ABSTRACT FROM AUTHOR]

Pulmonary hypertension (PH) is a chronic, progressive condition in which respiratory muscle dysfunction is a primary contributor to exercise intolerance and dyspnea in patients. Contractile function, blood flow distribution, and the hyperemic response are altered in the diaphragm with PH, and we sought to determine whether this may be attributed, in part, to impaired vasoreactivity of the resistance vasculature. We hypothesized that there would be blunted endothelium-dependent vasodilation and impaired myogenic responsiveness in arterioles from the diaphragm of PH rats. Female Sprague-Dawley rats were randomized into healthy control (HC, n = 9) and monocrotaline-induced PH rats (MCT, n = 9). Endothelium-dependent and -independent vasodilation and myogenic responses were assessed in first-order arterioles (1As) from the medial costal diaphragm in vitro. There was a significant reduction in endothelium-dependent (via acetylcholine; HC, 78 ± 15% vs. MCT, 47 ± 17%; P < 0.05) and -independent (via sodium nitroprusside; HC, 89 ± 10% vs. MCT, 66 ± 10%; P < 0.05) vasodilation in 1As from MCT rats. MCT-induced PH also diminished myogenic constriction (P < 0.05) but did not alter passive pressure responses. The diaphragmatic weakness, impaired hyperemia, and blood flow redistribution associated with PH may be due, in part, to diaphragm vascular dysfunction and thus compromised oxygen delivery which occurs through both endothelium-dependent and -independent mechanisms. [Display omitted] • Pulmonary hypertension (PH) induces diaphragm dysfunction and blood flow redistribution. • Diaphragm arteriolar responsiveness is impaired in PH rats. • Both endothelial function and vascular smooth muscle function are blunted with PH. • Diaphragm vascular dysfunction may predicate respiratory muscle failure in PH. [ABSTRACT FROM AUTHOR]

Exposure to cadmium may increase risk of urolithiasis, but the results remain inconclusive. This systematic review and meta-analysis aimed to access the association between cadmium exposure and urolithiasis. We searched Medline/PubMed, Embase, Web of Science Core Collection, and Cochrane Central for studies. The primary outcome was the incidence of urolithiasis compared to reference groups. We used relative risk as the summary effect measure. This meta-analysis included eight observational studies and divided into 39 study populations. Among 63,051 subjects, 5018 (7.96%) individuals had urolithiasis. The results indicated that people with an increment of 0.1 μg/g creatinine in urinary cadmium had a 2% increased risk of urolithiasis (pooled relative risk [RR], 1.02; 95% confidence interval [CI], 1.01–1.03) and there is no difference in the risk of urolithiasis in high and low cadmium exposure levels. Meanwhile, people with an increment of 0.1 μg/L in urinary cadmium had a 4% increased risk of urolithiasis (pooled RR, 1.04; 95% CI, 1.02–1.07). Our findings also showed similar associations in both sex, different region (Sweden, China, and Thailand), general and occupational population. The results indicate that cadmium exposure was significantly associated with an elevated risk of urolithiasis. Therefore, it is imperative to take steps to minimize cadmium exposure. • Higher urinary cadmium is associated with an increased incidental urolithiasis risk. • The association could be found in different genders, regions, and exposure settings. • Standardized rate ratio aids urolithiasis comparison in 39 unique study populations. [ABSTRACT FROM AUTHOR]

The lung represents a frequent site of spread for metastatic melanoma, which has historically been managed with surgical resection achieving promising outcomes. We hypothesized that the role of surgery in the management of melanoma pulmonary metastases (MPM) is evolving among the development of less invasive diagnostic and novel systemic therapeutic strategies. A single-center thoracic surgery database was reviewed and patients who underwent surgical resection of MPM between 1998 and 2019 were identified. Demographic, clinicopathologic, and surgical data were collected and analyzed, as were the annual volumes and indications for surgical resection. A Cochran-Armitage test was used to assess the trend in surgical indication. Three hundred and seventy seven surgical procedures for MPM were performed during the years of study in the care of 347 patients. Patients were predominantly male, with a mean age of 59.3 y. The mean number of annual resections was 17 and while this number initially increased from six in 1998 to a peak of 39 cases in 2008, a decline was subsequently observed. Diagnostic resection decreased from 22% in 1998-1999 to 5% at the peak of procedures in 2008-2009 and to 0 in 2018-2019 (P = 0.02). Curative resection increased from 44% in 1998-1999 to 73% in 2008-2009 (P < 0.001) and remained the dominant reason for surgery in later years. Surgical indications in the management of MPM have transformed in conjunction with systemic modalities, and the volume of resections has decreased in the modern era. Despite innovations in systemic management and shifting goals of operative interventions, surgeons continue to play a vital role in caring for these patients with an advanced disease. • Therapies for melanoma pulmonary metastases (MPM) continue to evolve. • Surgical indications for MPM are changing in parallel with treatment options. • Recent years have seen greater emphasis on curative resection and tissue harvest. • Surgical resection remains a valuable tool in the management of MPM. [ABSTRACT FROM AUTHOR]

Traumatic injury is the leading cause of pediatric mortality and morbidity in the United States. Pediatric trauma survivors requiring inpatient rehabilitation (IPR) require coordinated, multispecialty follow-up. Knowledge of the nature and level of disability is necessary for planning this continued care that is specific to the needs of pediatric trauma patients. This study aims to describe the outcomes of pediatric and adolescent trauma patients using measures of functional progression. A retrospective review of trauma patients aged ≤18 y admitted to IPR between January 2018 and December 2020 at the only certified pediatric rehabilitation center in the region was performed. Ninety five children and adolescents were admitted to IPR after traumatic injury with diagnoses of multitrauma (MT, N = 18), traumatic brain injury (TBI, N = 59), and spinal cord injury (SCI, N = 18). School aged children returned to school at high rates for all injury types (MT: 86.7%, TBI: 97.4%, SCI: 93.8%, P = ns). All groups had similar hospital and rehabilitation length of stay, and most patients required a durable medical equipment at discharge (79%). Using pediatric functional independence measure scoring progression from admission to discharge from IPR, SCI patients made significant improvement in bladder function and the least improvement in stair function. Patients sustaining a TBI made significant improvement in memory and comprehension tasks. Pediatric and adolescent trauma patients admitted to IPR had a positive progression during their therapy but required variable ongoing care depending on the mechanism of injury. Excellent rates of returning to school were seen across the three injury types. [ABSTRACT FROM AUTHOR]

• Derived a new class of nonlinearly stable flux reconstruction schemes in split form. • Nonlinearly stable flux reconstruction correction functions must be applied on the nonlinear volume terms. • Flux reconstruction in split form with the correction functions only reconstructing the surface is divergent. • The split form must be embedded within the discrete stiffness operator for nonlinear stability. The flux reconstruction (FR) method has gained popularity in the research community as it recovers promising high-order methods through modally filtered correction fields, such as the discontinuous Galerkin method, amongst others, on unstructured grids over complex geometries. Moreover, FR schemes, specifically energy stable FR (ESFR) schemes also known as Vincent-Castonguay-Jameson-Huynh schemes, have proven attractive as they allow for design flexibility as well as stability proofs for the linear advection problem on affine elements. Additionally, split forms have recently seen a resurgence in research activity due to their resultant nonlinear (entropy) stability proofs. This paper derives for the first time nonlinearly stable ESFR schemes in split form that enable nonlinear stability proofs for, uncollocated, modal, ESFR split forms with different volume and surface cubature nodes. The critical enabling technology is applying the splitting to the discrete stiffness operator. This naturally leads to appropriate surface and numerical fluxes, enabling both entropy stability and conservation proofs. When these schemes are recast in strong form, they differ from schemes found in the ESFR literature as the ESFR correction functions are incorporated on the volume integral. Furthermore, numerical experiments are conducted for Burgers' equation verifying that the new class of proposed ESFR split forms is nonlinearly stable in contrast to the standard split form ESFR approach. Lastly, the new ESFR split form is shown to obtain the correct orders of accuracy. [ABSTRACT FROM AUTHOR]

[Display omitted] • The poison frog genus Epipedobates is ∼11 My old and contains eight putative species. • Inconspicuous Epipedobates spp. are more deeply diverged genetically than aposematic spp. • We reorganize the E. boulengeri/espinosai species complex into four species. • We synonymize E. darwinwallacei with E. espinosai. • We provide the first genetic data for E. narinensis. Poison frogs (Dendrobatidae) are famous for their aposematic species, having a combination of diverse color patterns and defensive skin toxins, yet most species in this family are inconspicuously colored and considered non-aposematic. Epipedobates is among the youngest genus-level clades of Dendrobatidae that includes both aposematic and inconspicuous species. Using Sanger-sequenced mitochondrial and nuclear markers, we demonstrate deep genetic divergences among inconspicuous species of Epipedobates but relatively shallow genetic divergences among conspicuous species. Our phylogenetic analysis includes broad geographic sampling of the inconspicuous lineages typically identified as E. boulengeri and E. espinosai , which reveals two putative new species, one in west-central Colombia (E. sp. 1) and the other in north-central Ecuador (E. aff. espinosai). We conclude that E. darwinwallacei is a junior subjective synonym of E. espinosai. We also clarify the geographic distributions of inconspicuous Epipedobates species including the widespread E. boulengeri. We provide a qualitative assessment of the phenotypic diversity in each nominal species, with a focus on the color and pattern of inconspicuous species. We conclude that Epipedobates contains eight known valid species, six of which are inconspicuous. A relaxed molecular clock analysis suggests that the most recent common ancestor of Epipedobates is ∼11.1 million years old, which nearly doubles previous estimates. Last, genetic information points to a center of species diversity in the Chocó at the southwestern border of Colombia with Ecuador. A Spanish translation of this text is available in the supplementary materials. [ABSTRACT FROM AUTHOR]

Cryopreserved human heart valves fill a crucial role in the treatment for congenital cardiac anomalies, since the use of alternative mechanical and xenogeneic tissue valves have historically been limited in babies. Heart valve models have been used since 1998 to better understand the impact of cryopreservation variables on the heart valve tissue components with the ultimate goals of improving cryopreserved tissue outcomes and potentially extrapolating results with tissues to organs. Cryopreservation traditionally relies on conventional freezing, employing cryoprotective agents, and slow cooling to sub-zero centigrade temperatures; but it is plagued by the formation of ice crystals and cell damage upon thawing. Researchers have identified ice-free vitrification procedures and developed a new rapid warming method termed nanowarming. Nanowarming is an emerging method that utilizes targeted application of energy at the nanoscale level to rapidly rewarm vitrified tissues, such as heart valves, uniformly for transplantation. Vitrification and nanowarming methods hold great promise for surgery, enabling the storage and transplantation of tissues for various applications, including tissue repair and replacement. These innovations have the potential to revolutionize complex tissue and organ transplantation, including partial heart transplantation. Banking these grafts addresses organ scarcity by extending preservation duration while preserving biological activity with maintenance of structural fidelity. While ice-free vitrification and nanowarming show remarkable potential, they are still in early development. Further interdisciplinary research must be dedicated to exploring the remaining challenges that include scalability, optimizing cryoprotectant solutions, and ensuring long-term viability upon rewarming in vitro and in vivo. [ABSTRACT FROM AUTHOR]