1. The role of native cysteine residues in the oligomerization of KCNQ1 channels.
- Author
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Bates, Alison, Stowe, Rebecca B., Travis, Elizabeth M., Cook, Lauryn E., Dabney-Smith, Carole, and Lorigan, Gary A.
- Subjects
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POTASSIUM channels , *OLIGOMERIZATION , *MOLECULAR structure , *SPIN labels , *ELECTRON paramagnetic resonance , *LONG QT syndrome - Abstract
KCNQ1, the major component of the slow-delayed rectifier potassium channel, is responsible for repolarization of cardiac action potential. Mutations in this channel can lead to a variety of diseases, most notably long QT syndrome. It is currently unknown how many of these mutations change channel function and structure on a molecular level. Since tetramerization is key to proper function and structure of the channel, it is likely that mutations modify the stability of KCNQ1 oligomers. Presently, the C-terminal domain of KCNQ1 has been noted as the driving force for oligomer formation. However, truncated versions of this protein lacking the C-terminal domain still tetramerize. Therefore, we explored the role of native cysteine residues in a truncated construct of human KCNQ1, amino acids 100–370, by blocking potential interactions of cysteines with a nitroxide based spin label. Mobility of the spin labels was investigated with continuous wave electron paramagnetic resonance (CW-EPR) spectroscopy. The oligomerization state was examined by gel electrophoresis. The data provide information on tetramerization of human KCNQ1 without the C-terminal domain. Specifically, how blocking the side chains of native cysteines residues reduces oligomerization. A better understanding of tetramer formation could provide improved understanding of the molecular etiology of long QT syndrome and other diseases related to KCNQ1. • Exploration of oligomerization of Kv channels without C-terminal domain. • Nitroxide based spin labeling of native cysteine residues alters oligomerization. • C214 plays a role in the stability and or formation of KCNQ1 tetramers. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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