1. Utilizing protein-polyphenol molecular interactions to prepare moringa seed residue protein/tannic acid Pickering stabilizers.
- Author
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Huang, Zhilian, Liao, Liangkun, McClements, David Julian, Li, Jihua, Li, Ruyi, Zou, Ying, Li, Mi, and Zhou, Wei
- Subjects
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TANNINS , *SEED proteins , *MOLECULAR interactions , *PLANT polyphenols , *FOURIER transform infrared spectroscopy , *ISOTHERMAL titration calorimetry , *MORINGA - Abstract
The formation of complexes between plant proteins and polyphenols is being explored for the development for functional ingredients with new or improved properties. In this study, complexes were fabricated from moringa seed residue protein (MSRP) and tannic acid (TA). Fourier transform infrared spectroscopy and isothermal titration calorimetry suggested that the main interactions between MSRP and TA were hydrophobic and hydrogen bonding. The MSRP-TA complexes had near neutral wettability (θ ow = 86.1°), which meant that they could adsorb strongly to oil-water interfaces and thereby form and stabilize Pickering emulsions. The stability of the emulsions was improved by optimizing the MSRP:TA ratio. Confocal laser scanning microscopy indicated that higher tannic acid concentrations enhanced the thickness and compactness of the MSRP-TA interfacial layers formed around the oil droplets. The physicochemical properties of the emulsions were measured over a range of pH (3–9) and ionic strength (0–0.4 M NaCl) conditions. These results showed that the complexes were able to form stable emulsions at lower ionic strengths (<0.2 M) and higher pH values (pH 7–9). Overall, this study shows that self-assembly of MSRP and TA can improve the emulsifying properties of MSRP, which may increase its application as an emulsifier in foods. [Display omitted] • Interactions between MSRP and TA were hydrogen bonding and hydrophobic interaction. • Tuning ratio of MSRP-TA to 1:0.5, the complexes had tuned near neutral wettability. • Complexation of MSRP and TA improved emulsifying properties. • Pickering emulsion stability was improved by proper MSRP-TA ratio, pH, or ionic strength. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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