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Your search keyword '"Zhengzheng Duan"' showing total 4 results
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4 results on '"Zhengzheng Duan"'

1. Transcriptomic Profiling Reveals Complex Molecular Regulation in Cotton Genic Male Sterile Mutant Yu98-8A.

Author

Weiping Fang, Fu'an Zhao, Yao Sun, Deyi Xie, Li Sun, Zhenzhen Xu, Wei Zhu, Lirong Yang, Yuanming Zhao, Shuping Lv, Zhongjie Tang, Lihong Nie, Wu Li, Jianan Hou, Zhengzheng Duan, Yuebo Yu, and Xiaojie Yang

Subjects
Medicine, Science
Abstract

Although cotton genic male sterility (GMS) plays an important role in the utilization of hybrid vigor, its precise molecular mechanism remains unclear. To characterize the molecular events of pollen abortion, transcriptome analysis, combined with histological observations, was conducted in the cotton GMS line, Yu98-8A. A total of 2,412 genes were identified as significant differentially expressed genes (DEGs) before and during the critical pollen abortion stages. Bioinformatics and biochemical analysis showed that the DEGs mainly associated with sugars and starch metabolism, oxidative phosphorylation, and plant endogenous hormones play a critical and complicated role in pollen abortion. These findings extend a better understanding of the molecular events involved in the regulation of pollen abortion in genic male sterile cotton, which may provide a foundation for further research studies on cotton heterosis breeding.

Published
2015
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2. Imsnc761 and DDX6 synergistically suppress cell proliferation and promote apoptosis via p53 in testicular embryonal carcinoma cells

Author

Ping Ping, Fei Sun, Zhengzheng Duan, Xiansheng Zhang, and Guishuan Wang

Subjects
0301 basic medicine, p53, Male, Embryonal Carcinoma Stem Cells, RNA, Untranslated, proliferation, Cell, KEGG pathways, Biophysics, Biology, Biochemistry, DEAD-box RNA Helicases, 03 medical and health sciences, Testicular Neoplasms, Cell Line, Tumor, Proto-Oncogene Proteins, DDX6, Testis, medicine, Humans, KEGG, Molecular Biology, Gene, Research Articles, Cell Proliferation, Cell growth, apoptosis, Translation (biology), Cell Biology, Cell biology, Testicular Embryonal Carcinoma, 030104 developmental biology, medicine.anatomical_structure, HEK293 Cells, Apoptosis, imsnc761, Tumor Suppressor Protein p53, Function (biology), Research Article
Abstract

Intermediate-sized non-coding RNAs (imsncRNAs) have been shown to play important regulatory roles in the development of several eukaryotic organisms. In the present research, we selected imsncRNA 761 (imsnc761) as a research target. Expression analyses in a previous study showed that imsnc761 was down-regulated in maturation-arrested testis tissues as compared with the level in normal controls. In the present study, we found that imsnc761 could interact with DEAD-box helicase 6 (DDX6) to induce NTERA-2 (NT2 (testicular embryonal carcinoma cell)) cell apoptosis and proliferation inhibition via the p53 pathway. This interaction between imsnc761 and DDX6 also inhibited mitochondrial function and specific gene transcription and translation. To facilitate further research, we used label-free quantitation method to analyze the associated differences in Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways and biological processes. This confirmed the changes in several specific pathways, which matched our molecular experimental results.

Published
2018

3. The functional and predictive roles of miR-210 in cryptorchidism

Author

Zhengzheng Duan, Helong Huang, and Fei Sun

Subjects
0301 basic medicine, Male, Receptors, Cytoplasmic and Nuclear, Biology, Bioinformatics, Article, Male infertility, Andrology, 03 medical and health sciences, Mice, Downregulation and upregulation, Cell Line, Tumor, microRNA, Cryptorchidism, Testis, medicine, Animals, Humans, Reproductive system, Receptor, Spermatogenesis, Azoospermia, Regulation of gene expression, Multidisciplinary, medicine.disease, Spermatogonia, Up-Regulation, Repressor Proteins, MicroRNAs, 030104 developmental biology, HEK293 Cells, Nuclear receptor, Gene Expression Regulation, Biomarker (medicine)
Abstract

Idiopathic diseases of the reproductive system are important factors leading to male infertility. Many studies have shown that microRNAs (miRNAs) regulate the expression of multiple genes that play a significant role in spermatogenesis and development. We previously showed that microRNA-210 (miR-210) is one of the markedly upregulated microRNAs in the testes of sterile males with maturation arrest (MA). However, the role of miR-210 in spermatogenesis remains unknown. In this study, we found that miR-210 is highly expressed not only in patients with MA but also in patients with cryptorchidism. In addition, miR-210 inhibits the expression of Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) both in vitro and in vivo, particularly in cryptorchidic tissues. To facilitate further research, we established a mouse model of cryptorchidism and were surprised to discover that the miR-210 expression pattern was in accordance with that of patients with cryptorchidism. Thus, we propose that miR-210 may serve as a biomarker of cryptorchidism in clinical tests.

Published
2016

4. Imsnc761 and DDX6 synergistically suppress cell proliferation and promote apoptosis via p53 in testicular embryonal carcinoma cells.

Author

Zhengzheng Duan, Ping Ping, Guishuan Wang, Xiansheng Zhang, and Fei Sun

Subjects
*CANCER cell proliferation, *APOPTOSIS, *TESTICULAR cancer, *NON-coding RNA, *EUKARYOTIC cells
Abstract

Intermediate-sized non-coding RNAs (imsncRNAs) have been shown to play important regulatory roles in the development of several eukaryotic organisms. In the present research, we selected imsncRNA 761 (imsnc761) as a research target. Expression analyses in a previous study showed that imsnc761 was down-regulated in maturation-arrested testis tissues as compared with the level in normal controls. In the present study, we found that imsnc761 could interact with DEAD-box helicase 6 (DDX6) to induce NTERA-2 (NT2 (testicular embryonal carcinoma cell)) cell apoptosis and proliferation inhibition via the p53 pathway. This interaction between imsnc761 and DDX6 also inhibited mitochondrial function and specific gene transcription and translation. To facilitate further research, we used label-free quantitation method to analyze the associated differences in Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways and biological processes. This confirmed the changes in several specific pathways, which matched our molecular experimental results. [ABSTRACT FROM AUTHOR]

Published
2018
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