Search

OBJECTIVE: To investigate the role of TGF-β1 in autophagy and invasion ability in human gastric carcinoma cell line SGC7901. MATERIALS AND METHODS: Cultured SGC7901 cells were treated with different concentrations of TGF-β1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-β1 on invasion ability of SGC7901 cells was detected with transwell method. RESULTS: The results demonstrated that TGF-β1 was able to induced autophagy of SGC7901 cells in a dose-dependent manner. Autophagy inhibitor 3-MA could inhibit TGF-β1 upregulated autophagy. Furthermore, TGF-β1 significantly enhances the invasion ability of SGC7901 cells. However, autophagy inhibitor 3-MA could effectively reverse this process. CONCLUSIONS: TGF-β1 enhances SGC7901 cells migration by inducing autophagy. [ABSTRACT FROM AUTHOR]

Objective: To assess the prevalence of HIV infection and related factors in men who have sex with men (MSM) living for ≤5 years in Wenzhou of Zhejiang province. Methods: MSM who were aged ≥16 years, had lived in Wenzhou for ≥3 months and had anal sex and/or oral sex with men in the last 12 months were recruited through respondent-driven sampling (RDS) from February to October in 2015. The MSM recruited completed a questionnaire for the information collection on socio-demographic characteristics, sexual behavior, awareness of HIV and related intervention, mental health status. Blood samples were collected from them for serological detection of HIV and syphilis antibodies. Software SPSS 18.0 was used to analyze HIV infection and related factors. Results: A total of 454 MSM were investigated, 108 of them (23.7 % ) were HIV positive. There were 267 MSM who lived in Wenzhou for ≤5 years, and 73 of them (27.3 % ) were HIV positive. Most of them were workers and commercial servants aged ≥25 years with personal monthly income <4 000 yuan and educational level of junior high school or below. According to multiple logistics regression analysis, age of 25-68 years old ( OR =12.19, 95 %CI : 2.29-65.02), heterosexual behavior in recent 6 months ( OR =0.42, 95 %CI : 0.18-0.96), believing it was possible to be infected with HIV ( OR =0.06, 95 %CI : 0.01-0.95), believing it was impossible to be infected with HIV ( OR =0.03, 95 %CI : 0.01-0.35) and syphilis status ( OR =3.32, 95 %CI : 1.05-10.52) were the risk factors associated with HIV infection. Conclusion: Compared with MSM who lived in Wenzhou for >5 years, MSM who lived in Wenzhou for ≤5 years had higher HIV infection rate and higher prevalence of risk behavior. It is necessary to conduct targeted intervention among them.

Objective: To investigate the effect of humidex combined with mean temperature and relative humidity on the incidence of bacillary dysentery in Hefei. Methods: Daily counts of bacillary dysentery cases and weather data in Hefei were collected from January 1, 2006 to December 31, 2013. Then, the humidex was calculated from temperature and relative humidity. A Poisson generalized linear regression combined with distributed lag non-linear model was applied to analyze the relationship between humidex and the incidence of bacillary dysentery, after adjusting for long-term and seasonal trends, day of week and other weather confounders. Stratified analyses by gender, age and address were also conducted. Results: The risk of bacillary dysentery increased with the rise of humidex. The adverse effect of high humidex (90 percentile of humidex) appeared in 2-days lag and it was the largest at 4-days lag ( RR =1.063, 95 %CI : 1.037-1.090). Subgroup analyses indicated that all groups were affected by high humidex at lag 2-5 days. Conclusion: High humidex could significantly increase the risk of bacillary dysentery, and the lagged effects were observed.

Objective: To explore the correlation between quantitative value of joint bone scan by single photon emission computed tomography(SPECT) and serum bone metabolic markers in patients with active rheumatoid arthritis(RA). Method: Clinical data of 60 newly diagnosed RA patients were retrospectively collected in Department of Rheumatology and Immunology from May 2013 to December 2014, including 28 cases with medical history less than 6 months and 32 from 6 months to 2 years. Serum C-terminal telopeptide of type Ⅰ collgen(CTX-Ⅰ)and N-terminal propeptide of type Ⅰ collagen(PINP)were tested by chemiluminesence. SPECT whole body bone and target joint scan before treatment was done. Thirty-nine healthy subjects as control group received ultrasound, electrocardiogram, X-ray, and whole body bone imaging examination. Quantitative values of joint imaging were calculated for the statistical analysis. Result: Demographic data between RA group and control group were comparable ( P >0.05), including sample, sex, age and BMI. The joint SPECT value, CTX-Ⅰ and PINP levels were all significantly higher than those of control group ( P <0.01), which were 6.48±1.98 versus 3.73±1.16; (0.66±0.37) mg/L versus (0.58±0.21) mg/L; (46.35±28.15) mg/L versus (30.47±13.75) mg/L respectively. Joint SPECT values had positive correlations with serum CTX-Ⅰ levels in all RA patients, as well as PINP in patients with disease duration 6mon-2years. And the according correlation coefficients were 0.513, 0.495, 0.402( P <0.05). But SPECT value had no correlation with CTX-Ⅰ ( P =0.081) in patients with disease duration less than 6 mon.The correlation coefficient was 0.336. Conclusion: SPECT imaging quantitative values were positively correlated with serum bone metabolic parameters. Thus SPECT imaging alone or combined with bone markers are helpful in diagnosing active RA.