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Your search keyword '"Yi-wen Ou"' showing total 4 results
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4 results on '"Yi-wen Ou"'

1. Gut microbiota induced abnormal amino acids and their correlation with diabetic retinopathy

Author

Sheng-Qun Jiang, Su-Na Ye, Yin-Hua Huang, Yi-Wen Ou, Ke-Yang Chen, Jian-Su Chen, and Shi-Bo Tang

Subjects
proliferative retinopathy, gut microbiota, ruminococcaceae, amino acid metabolism, arginine, Ophthalmology, RE1-994
Abstract

AIM: To explore the correlation of gut microbiota and the metabolites with the progression of diabetic retinopathy (DR) and provide a novel strategy to elucidate the pathological mechanism of DR. METHODS: The fecal samples from 32 type 2 diabetes patients with proliferative retinopathy (PDR), 23 with non-proliferative retinopathy (NPDR), 27 without retinopathy (DM), and 29 from the sex-, age- and BMI- matched healthy controls (29 HC) were analyzed by 16S rDNA gene sequencing. Sixty fecal samples from PDR, DM, and HC groups were assayed by untargeted metabolomics. Fecal metabolites were measured using liquid chromatography-mass spectrometry (LC-MS) analysis. Associations between gut microbiota and fecal metabolites were analyzed. RESULTS: A cluster of 2 microbiome and 12 metabolites accompanied with the severity of DR, and the close correlation of the disease progression with PDR-related microbiome and metabolites were found. To be specific, the structure of gut microbiota differed in four groups. Diversity and richness of gut microbiota were significantly lower in PDR and NPDR groups, than those in DM and HC groups. A cluster of microbiome enriched in PDR group, including Pseudomonas, Ruminococcaceae-UCG-002, Ruminococcaceae-UCG-005, Christensenellaceae-R-7, was observed. Functional analysis showed that the glucose and nicotinate degradations were significantly higher in PDR group than those in HC group. Arginine, serine, ornithine, and arachidonic acid were significantly enriched in PDR group, while proline was enriched in HC group. Functional analysis illustrated that arginine biosynthesis, lysine degradation, histidine catabolism, central carbon catabolism in cancer, D-arginine and D-ornithine catabolism were elevated in PDR group. Correlation analysis revealed that Ruminococcaceae-UCG-002 and Christensenellaceae-R-7 were positively associated with L-arginine, ornithine levels in fecal samples. CONCLUSION: This study elaborates the different microbiota structure in the gut from four groups. The relative abundance of Ruminococcaceae-UCG-002 and Parabacteroides are associated with the severity of DR. Amino acid and fatty acid catabolism is especially disordered in PDR group. This may help provide a novel diagnostic parameter for DR, especially PDR.

Published
2024
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3. Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae

Author

Shuai Li, Wei Chen, Yi-wen Ou, Qin Ou, and Bei Li

Subjects
0301 basic medicine, Minimum bactericidal concentration, medicine.drug_class, 030106 microbiology, Cephalosporin, Biofilm, General Medicine, Microbial Sensitivity Tests, biochemical phenomena, metabolism, and nutrition, Biology, medicine.disease, biology.organism_classification, Microbiology, Agar dilution, Integrons, 03 medical and health sciences, Minimum inhibitory concentration, Klebsiella pneumoniae, Biofilms, medicine, Scutellaria, Scutellaria baicalensis, Klebsiella pneumonia
Abstract

Objective To explore the effects of Scutellaria baicalensis on activity and biofilm formation of Klebsiella pneumonia (Kp).Methods The broth and agar dilution Methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of Scutellaria baicalensis for TW518. VITEK-32 system was used to assay TW518 susceptibility to antibiotics. Kp biofilms were formed in vitro and stained with BacLight Live/Dead stain. The class integron geneⅠ1 mRNA expression was analyzed with RT-PCR.Results The minimum inhibitory concentration of Scutellaria baicalensis on TW518 identified as a Kp colony was 32 mg/ml, and minimum bactericidal concentration was 64 mg/ml. Scutellaria baicalensis and broad-spectrum penicillin, cephalosporin, quinolones, or beta-lactamase had synergistic bactericidal effects. Biofilm formation activity of Kp treated with Scutellaria baicalensis was significantly lower than that of the control group. And class integron geneⅠ1 mRNA expression of TW518 was significantly inhibited by Scutellaria baicalensis.Conclusions Scutellaria baicalensis has sterilization effect on Kp, and Scutellaria baicalensis could effectively inhibit Kp biofilm formation with prolonged treatment. Scutellaria baicalensis might inhibit Kp biofilm formation through down-regulating integron geneⅠ1 expression.

Published
2016

4. Dynamic analysis of the human brain with complex cerebral sulci

Author

Yi-Te Wu, Ke-Tien Yen, Jung-Ge Tseng, Yi-Wen Ou, and Bo-Wun Huang

Subjects
Head (linguistics), 0206 medical engineering, Finite Element Analysis, Bioengineering, 02 engineering and technology, Brain damage, Applied Microbiology and Biotechnology, Models, Biological, 03 medical and health sciences, 0302 clinical medicine, Imaging, Three-Dimensional, Medicine, Humans, Computer Simulation, Brain Concussion, business.industry, Brain, General Medicine, Human brain, Human body, Anatomy, 020601 biomedical engineering, Skull, medicine.anatomical_structure, Brain Injuries, medicine.symptom, business, 030217 neurology & neurosurgery, Software, Biotechnology, Research Paper
Abstract

The brain is one of the most vulnerable organs inside the human body. Head accidents often appear in daily life and are easy to cause different level of brain damage inside the skull. Once the brain suffered intense locomotive impact, external injuries, falls, or other accidents, it will result in different degrees of concussion. This study employs finite element analysis to compare the dynamic characteristics between the geometric models of an assumed simple brain tissue and a brain tissue with complex cerebral sulci. It is aimed to understand the free vibration of the internal brain tissue and then to protect the brain from injury caused by external influences. Reverse engineering method is used for a Classic 5-Part Brain (C18) model produced by 3B Scientific Corporation. 3D optical scanner is employed to scan the human brain structure model with complex cerebral sulci and imported into 3D graphics software to construct a solid brain model to simulate the real complex brain tissue. Obtaining the normal mode analysis by inputting the material properties of the true human brain into finite element analysis software, and then to compare the simplified and the complex of brain models.

Published
2016

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