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Deoxynivalenol (DON) contamination causes the grievous injury in public and animal health, poultry suffer from the greater toxin challenge. Probiotic have been considered as a potential way to mitigate the deleterious effects of DON. In this study, a total of 144 1-day-old Arbor Acres chickens were randomly assigned into 3 groups: control group, DON group (5 mg/kg DON diet), DJ group (1×109cfu Lactiplantibacillus plantarumJM113/kg DON diet). The results showed that Lactiplantibacillus plantarumJM113 (L. plantarumJM113) increased the growth performance of 21-day-old broilers that challenged by the DON (P< 0.05), and the DON-induced disorder of jejunal morphology was recovered in DJ group (P< 0.05). Compared with the DON group, the mRNA and protein levels of Nrf2 and NQO-1 were upregulated in jejunum of DJ group broilers (P< 0.05). Meanwhile, administration of L. plantarumJM113 effectively increased the expression level of barrier-related genes, and the protein abundance of occludin and claudin1 (P< 0.05). L. plantarumJM113 restored the mRNA and protein abundance of PCNA, and proliferation-linked gene (Lgr5and Bmi1) expression levels in jejunum of DON-insulted broilers (P< 0.05). Furthermore, administration of L. plantarumJM113 significantly enhanced the relative abundance of s_Limosilactobacillus_reuteriin jejuna of DON-challenged broilers (P< 0.05). Spearman correlation analysis showed that s_Limosilactobacillus_reuteriwas positively associated with the jejunal barrier related genes (P< 0.05). In conclusion, L. plantarumJM113 alleviated the toxic effects of DON by regulating the jejunal function through microbial adjustment. Our findings proposed a viable approach to mitigating the adverse effects of deoxynivalenol exposure in broilers.