Search

We herein report 2 cases of herpes simplex keratitis after trans-epithelial photorefractive keratectomy. Patients' medical histories, symptoms, signs, clinical examination results, diagnosis and treatment were showed in detail. Following precision diagnosis and medical intervention, including topical and systemic antiviral treatmented for 1 to 2 weeks. The two patients were cured with full reepithelialization without corneal scar.本文报道了Trans-PRK术后单纯疱疹病毒性角膜炎患者2例，详细记述了患者的病史、症状、体征、辅助检查结果和诊疗过程。经过及时、确切的诊断和充分的治疗，患者经1~2周的局部联合全身的抗病毒药物治疗后角膜上皮完全修复，无角膜瘢痕。.

This study aimed to explore the effects of Wenyang Zhenshuai granules (WZG) on the morphology of cardiomyocytes, cell viability, and the expression of key mitochondrial autophagy proteins in the doxorubicin-induced model of H9c2 cardiomyocyte injury. Cardiomyocytes were cultured for 44 h and divided into 4 groups: intact control, doxorubicin-injured cells (DOX), doxorubicin-injured cells treated with WZG (DOX+WZG), and doxorubicin-injured cells treated with valsartan (DOX+valsartan; reference group). The morphology of cardiomyocytes was analyzed under an inverted microscope; cardiomyocyte survival rate was determined by MTT assay. The expression of the key mitochondrial autophagy proteins (PINK1, parkin, LC3-II, and prohibitin-2) was analyzed by Western blotting. WZG down-regulated the expression of the key mitochondrial autophagy proteins in DOX-injured cells, which may be one of the important mechanisms for regulating ventricular remodeling and cardiomyocyte apoptosis.

Water hyacinth (Eichhornia crassipes (Mart.) Solms), an invasive plant, is used to control algae-polluted water, but the mechanism among which is not clear. In this study, the allelopathic effects of water hyacinth on the growth of Microcytis aeruginosa were investigated. The physiological changes of M. aeruginosa such as chlorophyll content, superoxide dismutases (SODs), peroxidases (PODs), total protein contents (TPCs), and MDA contents were also determined. Our results showed that higher density of water hyacinth extracts and ethyl acetate fractions all showed significant allelopathic inhibition on the growth of M. aeruginosa. The ethyl acetate fraction considerably reduced the algae chlorophyll content. The higher concentration used of ethyl acetate fraction, the lower the chlorophyll content of algae. The activity of SODs and PODs were quite reduced after 10-day treatment by ethyl acetate fraction. The TPCs and MDA contents were also found to be decreased after 4-day treatment. It is inferred that water hyacinth inhibited the algal growth by released allelochemicals and affected diversity of biochemical and physiological attributes, and also lead to the membrane injury in the M. aeruginosa cells.

To explore the correlation between angiotensin II type 1 receptor (AT1R) gene polymorphism and epilepsy secondary to cerebral infarction and its significance for the diagnosis of this disease.A total of 200 patients with epilepsy secondary to cerebral infarction were enrolled from our hospital as observation group, and 200 patients without epilepsy after cerebral infarction as control group. Genomic deoxyribonucleic acids (DNAs) were extracted from the peripheral blood of the subjects, and the polymorphic regions at AT1R gene loci rs380400, rs1799870, rs12721273, and rs55707609 were amplified via polymerase chain reaction (PCR) and sent to the company for sequencing. The concentration of farnesyl diphosphate synthase (FDPS) was determined using enzyme-linked immunosorbent assay (ELISA) kit, and activated partial thromboplastin time (APTT) and prothrombin time (PT) were measured in the Laboratory Department.There were no differences in the allele distributions at AT1R gene loci rs380400 (p=0.070), rs179987 (p=0.0.280), and rs55707609 (p=0.046), but in the allele distribution at rs12721273 (p=0.001) between control group and observation group, and observation group exhibited a significantly lower frequency of allele G in cerebral infarction patients than control group [153 (0.383) vs. 198 (0.495)]. The frequency of genotype GT at rs12721273 was lower [71 (0.355)] and that of genotype TT was evidently higher [88 (0.440)] in observation group (p=0.000). Control group showed a notably lower frequency of genotype AA [47 (0.235)] and a markedly higher frequency of genotype AT [110 (0.550)] at rs55707609 (p=0.000). Observation group exhibited a substantially lower frequency of recessive model AG+GG [128 (0.640)] (p=0.037), and a notably higher frequency of homozygous model AA [72 (0.360)] (p=0.048) at AT1R gene locus rs380400, a remarkably lower frequency of dominant model GG+GT [112 (0.560)] (p=0.002) at rs12721273, and a significantly lower frequency of recessive model AT+TT [126 (0.630)] (p=0.000) and a considerably lower frequency of heterozygous model AT [84 (0.420)] (p=0.026) at rs55707609. The frequencies of AT1R gene haplotypes ACGA (p=0.001), ACGT (p=0.045), ACTT (p=0.000), ATTT (p=0.048), GCTA (p=0.000), and GTGA (p=0.005) in observation group were distinctly higher than those in control group, and the frequencies of the haplotypes ACTA (p=0.000) and ATTA (p=0.029) were evidently lower than those in control group. The loci rs12721273 and rs1799870 showed a significant association (D'=0.783), and APTT was considerably correlated with genotype AG at rs380400 (p=0.042), PT with genotype CC at rs1799870 (p=0.002) and FDPS with genotype AA at rs55707609 (p=0.015).The polymorphisms of AT1R gene loci rs380400, rs1799870, rs12721273, and rs55707609 are correlated with the susceptibility to epilepsy secondary to cerebral infarction.

Inflammatory accumulation in epicardial adipose tissue (EAT) may influence the formation and development of coronary artery disease (CAD). EAT macrophages exhibit M1 polarization and the secretion of a large number of inflammatory factors in CAD patients. Emerging data demonstrate that Krüppel-like factor-7 (KLF7), contributes to the regulation of adipocyte differentiation and the secretion of adipose tissue inflammation. However, the function of KLF7 in EAT inflammation still remains to be uncovered. This study aims to investigate the role of KLF7 in macrophage activation in EAT.The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in cell supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expression levels were measured by Real Time-PCR. The protein expression level was detected by Western blot.The expression of inflammatory factors and KLF7 were markedly increased in CAD EAT than non-CAD EAT. KLF7 is highly expressed in human THP-1-derived macrophages induced by inflammatory stimuli, such as LPS. The knockdown of KLF7 inhibited the release of inflammatory factors and significantly decreased the expression of KLF7 in human THP-1-derived macrophages stimulated by LPS. Moreover, transfection with KLF7-siRNA caused the marked inhibition of LPS-induced phosphorylation of JNK-MAPKs and also suppressed the levels of p-p65 and inhibited the activation of p-IκBα.Taken together, these results indicate that KLF7 enhances macrophage activation, mediated by JNK-NF-κB signaling pathways in EAT. This suggests that KLF7 may be a potential therapeutic target for cardiovascular diseases such as CAD.

Despite physiological importance of aldonic sugar acids for living organisms, little is known about metabolic pathways of these compounds. Here, we investigated the functional diversity of homologs of L-threonic acid dehydrogenase (ThrDH; UniProt ID: Q0KBC7), an enzyme composed of two NAD-binding domains (PF14833 and PF03446). Ten ThrDH homologs with different genomic context were studied; seven new enzymatic activities were identified, such as (R)-pantoate dehydrogenase, L-altronic acid dehydrogenase, 6-deoxy-L-talonate dehydrogenase, L-idonic acid dehydrogenase, D-xylonic acid dehydrogenase, D-gluconic acid dehydrogenase, and 2-hydroxy-3-oxopantoate reductase activities. Two associated metabolic pathways were identified: L-idonic acid dehydrogenase was found to be involved in the degradation of L-idonic acid through oxidation/decarboxylation in Agrobacterium radiobacter K84, while 2-hydroxy-3-oxopantoate reductase was found to participate in D-glucarate catabolism through dehydration/cleavage in Ralstonia metallidurans CH34.

Diamond samples containing silicon and nitrogen are shown to be heavily photochromic, with the dominant visible changes due to simultaneous change in total ${\mathrm{SiV}}^{0/\ensuremath{-}}$ concentration. The photochromism treatment is not capable of creating or destroying SiV defects, and thus we infer the presence of the optically inactive ${\mathrm{SiV}}^{2\ensuremath{-}}$ . We measure spectroscopic signatures we attribute to substitutional silicon in diamond, and identify a silicon-vacancy complex decorated with a nearest-neighbor nitrogen $\mathrm{SiVN}$, supported by theoretical calculations.

目的 分析造血干细胞移植（HSCT）治疗血管免疫母细胞性T细胞淋巴瘤（AITL）的临床疗效。 方法 回顾性分析2007年6月至2017年6月期间在国内八所医院接受HSCT的AITL患者。 结果 共有19例患者纳入本研究，其中男13例，女6例，中位年龄50（32~60）岁；自体造血干细胞移植（auto-HSCT）12例，异基因造血干细胞移植（allo-HSCT）7例（均为同胞全相合供者，其中2例为auto-HSCT后复发患者）。auto-HSCT组移植前疾病处于完全缓解（CR）、部分缓解（PR）状态者分别为5例、7例；allo-HSCT组患者移植前疾病处于PR、疾病进展（PD）状态者分别为2例、3例。失访2例（均为auto-HSCT组），中位随访时间46.5（1~100）个月。allo-HSCT组3例患者发生急性移植物抗宿主病（GVHD）（Ⅰ度2例，Ⅱ度1例），5例发生慢性GVHD（局限型2例，广泛型3例）。auto-HSCT组死亡4例（原发病复发死亡3例），allo-HSCT组死亡3例（移植相关死亡2例，原发病复发死亡1例）。auto-HSCT组（10例）、allo-HSCT组（7例）的3年总生存率分别为56%（95%CI 32%~100%）、57%（95%CI 30%~100%）（P=0.979）；3年累积无进展生存率分别为34%（95%CI 14%~85%）、57%（95%CI 30%~100%）（P=0.451）。 结论 auto-HSCT和allo-HSCT均是AITL的有效治疗方法。

This work presents a study on the controlled growth of WO

To understand the distribution of nerve fibers and the types of neural cells inWhole worms were subjected to silver staining, histochemical staining and hematoxylin-eosin (HE) staining, and the nervous systems of the worms were observed.There were 3 types of neural cells in the worm head near the cerebral ganglion, including unipolar, bipolar and multipolar neurons, which were divided into 7 types according to the morphology. There was a nerve network on the surface of pharynx and intestinal tract, as well as the reproductive organ, including testis, ovary, lower uterus and penis sac. The nerve network was consisted of circular and longitudinal nerve fibers, and the structure of the nerve network around the mouth was similar to central nerve.The structure of the

As a promising engineering material, high-entropy alloys (HEAs) CrFeCoNi system has attracted extensive attention worldwide. Their cast alloys are of great importance because of their great formability of complex components, which can be further improved through the transition of the columnar to equiaxed grains and grain refinement. In the current work, the influence of C contents on the grain structures and mechanical properties of the as-cast high-entropy alloy CrFeCoNi was chosen as the target and systematically studied via a hybrid approach of the experiments and thermodynamic calculations. The alloys with various C additions were prepared by arc melting and drop cast. The as-cast macrostructure and microstructure were characterized using optical microscopy, scanning electron microscopy, and transmission electron microscopy. The cast HEAs transform from coarse columnar grains into equiaxed grains with the C level increased to ≥ 2 at. pct and the size of equiaxed grains is further decreased with the increasing C addition. It is revealed that the interdendritic segregation of Cr and C results in grain boundary precipitation of M23C6 carbides. The grain refinement is attributed to the additional constitutional supercoiling from the C addition. The yield stress and tensile strength at room temperature are improved due to the transition of columnar to equiaxed grains and grain refinement.

Recently, porous carbon materials have attracted attention due to their versatile properties. In this study, a hierarchical porous N-doped carbon (NHPC) was successfully synthesized as a catalyst support using a facile method. A large amount of Pd nanoparticles were uniformly loaded on the NHPC with an average diameter of about 4.45 nm. The synthesized Pd/NHPC nanocatalyst exhibited excellent stability and reusability toward the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) at room temperature. The uniformity and high reusability of Pd/NHPC was mainly attributed to the abundant doped nitrogen as a coordinated site to largely stable Pd nanoparticles. Herein, the excellent properties of NHPC provided ideas for the fabrication of other NHPC supported noble nanoparticles, which can be applied in various catalytic reductive reactions.

Both atrial fibrillation (AF) and heart failure (HF) are increasingly prevalent and related to high hospitalization rate and mortality. AF is a cause as well as a consequence of HF, with complicated interactions resulting in impairment of cardiac systolic and diastolic function. Conversely, the complex structural and neurohormonal alterations in HF contribute to the occurrence and development of AF. However, the molecular mechanism remains unclear. This study aims to explore the effect of Exchange-protein activated by cAMP 1 (EPAC1) on AF in isoproterenol (ISO)-induced HF and the potential molecular mechanism.Mice and cultured isolated adult cardiomyocytes were treated with ISO and or not EPAC1 inhibitor CE3F4. Programmed electrical stimulation (PES) was performed to induce AF. EPAC1 expression was determined by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Western blot. Cellular electrophysiology was examined by whole cell patch clamp.Both mRNA and protein levels of EPAC1 were upregulated in HF mice. ISO increased the AF susceptibility, and the negative effect was deteriorated by CE3F4. ISO mediated high AF susceptibility of HF via prolonging action potential and exciting L-type calcium channel (LTCC). These could also be reversed by CE3F4 treatment.EPAC1 increased the AF susceptibility in ISO-induced HF mouse model via alternating LTCC.

Excessive zinc (Zn) uptake is toxic to both plants and animals. 24-Epibrassinolide (EBL), a plant steroid hormone, plays a pivotal role in regulating plant resistance to various stresses. In this s...

This work presents a study on the controlled growth of WO3 nanowires via chemical vapor deposition without catalyst, and their potential applications in visible photodetectors. The influence of growth conditions on the morphology of WO3 nanowires is studied in order to understand the growth mechanism of WO3 nanowires, and ultra-long (60 [Formula: see text], the longest one ever reported) WO3 nanowires with a spindle shape are achieved by optimizing the growth conditions. It was found that the length of WO3 nanowires increases from 15 [Formula: see text] to 60 [Formula: see text] with increasing the argon carrier gas flow rate from 30 sccm to 90 sccm, and then saturates with further increasing the argon carrier gas flow rate. However, the length of WO3 nanowires reduces from 60 [Formula: see text] to 19 [Formula: see text] with increasing the tube inner pressure from 2.5 Torr to 3.5 Torr. The photoconductor detectors based on WO3 single nanowires present excellent device performance with a responsivity as high as 19 A W-1 at a bias of 0.1 V, a detectivity as high as 1.06 × 1011 Jones, and a response (rising and decay) time as short as 8 ms under the illumination of a 404 nm laser. These results indicate the great potential of WO3 nanowires for applications in fabricating high performance visible photodetectors.

The effects of foliar application of 24-epibrassinolide (EBR) on chlorophyll content, oxidative damage, and antioxidant capacity in eggplant (Solanum melongena L.) seedlings during a short-term chilling stress and subsequent recovery were investigated. The eggplant seedlings pretreated with 0.1 μM EBR were exposed to 5 °C for 12 h in the dark and then recovered under a normal temperature for another 12 h. The results show that EBR alleviated a chilling-induced oxidative stress reflected by a decrease in malondialdehyde and H2O2 content, O2 ·− production rate, and an increase in chlorophyll content and activities of superoxide dismutase, guaicol peroxidase, and catalase. The EBR pretreatment also promoted activities of ascorbate peroxidase, glutathione reductase, dehydroascorbate reductase, as well as the accumulation of ascorbic acid (AsA) and glutathione (GSH) involved in the AsA-GSH cycle. Moreover, after the12 h recovery, EBR plus chilling treated leaves showed no significant changes in content of MDA and chlorophyll a, lower reactive oxygen species (ROS) content, and greater activities of AsA-GSH cycle enzymes than in seedlings only chilled. The results suggest that EBR alleviated an oxidative damage caused by the dark chilling stress and accelerated a recovery rate mainly through increasing the ROS scavenging system including the AsA-GSH cycle.

SummaryBrassinosteroids (BRs) have been implicated in the responses of plants to various environmental stresses.To explore the mechanism(s) of BRs in the tolerance of plants to low temperatures, 24-epibrassinolide (EBR)-induced changes in chlorophyll concentrations, gas exchange parameters, chlorophyll fluorescence, and anti-oxidant defence systems in eggplant (Solanum melongena L.) were studied under chilling stress conditions. Seedlings were exposed to 10°C/5°C (day/night) chilling temperatures for 8 d, with or without a single spray of 0.1 ?M EBR applied 24 h beforehand.The EBR treatment partially overcame the inhibition of growth caused by 8 d of chilling stress.The application of EBR to chilled seedlings had a significant effect on photosynthesis, as reflected by increases in the concentrations of chlorophyll a, chlorophyll b, and total chlorophyll, the net photosynthetic rate (Pn), stomatal conductance (gs), the quantum efficiency of PSII (Fv/Fm), the efficiency of the excitation capture of open PSI...

HLA-A*02:07:09 is different from HLA-A*02:07:01 by a single nucleotide substitution at position 244.

RNA-seq data of hepatocellular carcinoma (HCC) was analyzed to identify critical genes related to the pathogenesis and prognosis.Three RNA-seq datasets of HCC (GSE69164, GSE63863 and GSE55758) were downloaded from Gene Expression Omnibus (GEO), while another dataset including 54 HCC cases with survival time was obtained from The Cancer Genome Atlas (TCGA). Differentially expressed genes (DEGs) were identified by significant analysis of microarrays (SAM) method using package samr of R. As followed, we constructed a protein-protein interaction (PPI) network based on the information in Human Protein Reference Database (HPRD). Modules in the PPI network were identified with MCODE method using plugin clusterViz of CytoScape. Gene Ontology (GO) enrichment analysis and pathway enrichment analysis were performed with DAVID. The difference in survival curves was analyzed with Kaplan-Meier (K-M) method using package survival.A total of 2572 DEGs were identified in the 3 datasets from GEO (GSE69164, GSE63863 and GSE55758). The PPI network was constructed including 660 nodes and 1008 edges, and 4 modules were disclosed in the network. Module A (containing 244 DEGs) was found to related to HCC closely, which genes were involved in transcription factor binding, protein metabolism as well as regulation of apoptosis. Nine hub genes were identified in the module A, including PRKCA, YWHAZ, KRT18, NDRG1, HSPA1A, HSP90AA1, HSF1, IKGKB and UBE21. The network provides the protein-protein interaction of these critical genes, which were implicated in the pathogenesis of HCC. Survival analysis showed that there is a significant difference between two groups classified by the genes in module A. Further Univariate Cox regression analysis showed that 72 genes were associated with survival time significantly, such as NPM1, PRKDC, SPARC, HMGA1, COL1A1 and COL1A2.Nine critical genes related to the pathogenesis and 72 potential prognostic markers were revealed in HCC by the network and module analysis of RNA-seq data. These findings could improve the understanding of the pathogenesis and provide valuable information to further investigate the prognostic markers of HCC.

Wind-solar hybrid generation system can significantly improve the utilization efficiency of intermittent renewable energy as it can make full use of the complementarity of wind-solar. Meanwhile, it will affect the low frequency characteristics of power system. Effect of different wind-solar accommodation conditions on low frequency oscillation characteristics of power system are analyzed based on IEEE 2-area 4-unit system. Eigenvalue analysis and dynamic time-domain simulation are adopted to figure out the influence, including different factors such as separate integration, grid-connected integration points and hybrid capacity ratios of wind-solar.

To use the 3.0T susceptibility-weighted imaging (SWI) for universality analysis of the "swallow tail" appearance in the substantia nigra of non-Parkinson disease and discuss its lack of the value of imaging diagnosis of Parkinson disease (PD).Take 3.0TMR SWI in 60 PD patients (Group PD) and non-PD volunteers (Group N-PD), on the map of range analyze morphology and number of "swallow tail" appearance in substantia nigra of N-PD group volunteers, and compare the performance image of the corresponding region of the patients in the PD group.After, 15 patients with lesions in the brain stem and significant motion artifacts were excluded. Forty-nine cases of group N-PD (96.08%) had typical "swallow tail" appearance in the bilateral or unilateral substantia nigra compacta posterolateral. All 54 patients with group PD (100%) lacked the "drop" rear elliptical high signal.On the 3.0T SWI range map, the "swallow tail" appearance is ubiquitous in the substantia nigra of patients with non-PD. The deficiency of the signs has high sensitivity and specificity for PD diagnosis.

To assess the imaging features of nigrosomes-1 in the substantia nigra through 3T MR susceptibility weighted imaging (SWI) and its disease-specific changes for the diagnosis of Parkinson's disease (PD).A total of 116 subjects were included in this study and allocated into 3 groups: 54 patients diagnosed with PD were assigned to the PD group, 51 age- and sex-matched volunteers without PD served as the control N-PD group, and 11 clinically suspected PD patients were allocated to the undiagnosed (UD) group. All patients received 3.0T superconducting MRI scanning on xxx. The images were analyzed and compared to assess the ability of nigrosomes-1 signals to depict PD pathology.The signals of nigrosomes-1 were strong, droplet-like or oval in shape, and were found in 49 patients from the N-PD group (96.08%), on both sides of the SN (47 cases) and unilaterally (2 cases). In contrast, these signals were absent in all 54 cases from the PD group, and were undetected in 7 out of 11 cases in the UD group, 7 cases without the "drop" and 1 case with narrow strips of hyperintensity were clinically proven to PD, 2 cases with the typical hyperintensity were clinically proven to Parkinson's plus syndrome, 2 cases with slightly wider strip of hyperintensity were less sensitive to the drug levodopa.The absence of typical droplet-like or oval-shaped nigrosomes-1 signals in 3.0T MR SWI may prove useful in identifying PD and Parkinson's syndrome with high sensitivity and specificity.

With the development of photoelectric technology and single-chip microcomputer technology, objective optometry, also known as automatic optometry, is becoming precise. This paper proposed a presbyopia optometry method based on diopter regulation and Charge Couple Device (CCD) imaging technology and, in the meantime, designed a light path that could measure the system. This method projects a test figure to the eye ground and then the reflected image from the eye ground is detected by CCD. The image is then automatically identified by computer and the far point and near point diopters are determined to calculate lens parameter. This is a fully automatic objective optometry method which eliminates subjective factors of the tested subject. Furthermore, it can acquire the lens parameter of presbyopia accurately and quickly and can be used to measure the lens parameter of hyperopia, myopia and astigmatism.