12 results on '"Wu Liang-huan"'
Search Results
2. Effects of ammonium application rate on uptake of soil adsorbed amino acids by rice
- Author
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Cao, Xiao-chuang, Ma, Qing-xu, Wu, Liang-huan, Zhu, Lian-feng, and Jin, Qian-yu
- Published
- 2016
- Full Text
- View/download PDF
3. Experimental Determination of Silicon Isotope Fractionation in Rice
- Author
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Sun, Yan, primary, Wu, Liang-huan, additional, and Li, Xiao-yan, additional
- Published
- 2016
- Full Text
- View/download PDF
4. Effects of Biochemical Inhibitors on Transformation of Urea Nitrogen in Yellow Clayey Soil
- Author
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Zhou, Xuan, primary, Wu, Liang-Huan, additional, Lu, Ruo-Hui, additional, and Dai, Feng, additional
- Published
- 2015
- Full Text
- View/download PDF
5. Advances in studies of absorption and utilization of amino acids by plants: A review.
- Author
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CAO Xiao-chuang, WU Liang-huan, MA Qing-xu, and JIN Qian-yu
- Abstract
Plant can directly take up the intact amino acids, thus bypass the microbial mineralization of organic nitrogen. As an excellent carbon and nitrogen source, there exists competition for amino acid absorption between plant roots and soil microorganisms. And the total flux of amino acids in soil may be enormous due to the extensive sources and short hall-life. Studies on amino acid nitrogen nutritional contribution for plant by the technique of nitrogen isotopic tracer, has become a research topic in recent years, which will help us better understand the principle of soil fertility. This paper summarized the recent researches on amino acid morphological characteristics in soil and its metabolic mechanism and nitrogen nutritional contribution for plant in different ecosystems, and discussed the present status and development trend of the amino acid circulation mechanism in the plant-soil-microorganism ecosystem and its bioavailability for plant. Finally, the topics of environmental regulating mechanism of amino acid bioavailability, amino acid carbon-nitrogen metabolism, and how to improve the field organic nitrogen management were all the core issues to be resolved. [ABSTRACT FROM AUTHOR]
- Published
- 2015
6. Maintaining KEAP1 levels in retinal pigment epithelial cells preserves their viability during prolonged exposure to artificial blue light.
- Author
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Li CH, Yang TM, Fitriana I, Fang TC, Wu LH, Hsiao G, and Cheng YW
- Abstract
Exposure to artificial blue light, one of the most energetic forms of visible light, can increase oxidative stress in retinal cells, potentially enhancing the risk of macular degeneration. Retinal pigment epithelial (RPE) cells play a crucial role in this process; the loss of RPE cells is the primary pathway through which retinal degeneration occurs. In RPE cells, Kelch-like ECH-associated protein 1 (KEAP1) is located in both the nucleus and cytosol, where it binds to nuclear factor erythroid 2-related factor 2 (NRF2) and p62 (sequestosome-1), respectively. Blue light exposure activates the NRF2-heme oxygenase 1 (HMOX1) axis through both canonical and noncanonical p62 pathways thereby reducing oxidative damage, and initiates autophagy, which helps remove damaged proteins. These protective responses may support the survival of RPE cells. However, extended exposure to blue light drastically decreases the viability of RPE cells. This exposure diminishes the ability of KEAP1 to bind to p62 and reduces the level of KEAP1. Inhibition of autophagy does not prevent KEAP1 degradation, the NRF2-HMOX1 axis, or blue-light-induced cytotoxicity. However, proteasome inhibitor along with a transient increase in the amount of KEAP1 in RPE cells, partially restores the p62-KEAP1 complex and reduces blue-light-induced cytotoxicity. In vivo studies confirmed the downregulation of KEAP1 in damaged RPE cells. Mice subjected to periodic blue light exposure exhibited significant atrophy in the outer retina, particularly in the peripheral areas. Additionally, there was a significant decrease in c-wave electroretinography and pupillary light reflex, indicating functional impairments in both visual and nonvisual physiological processes. These data underscore the essential role of KEAP1 in managing oxidative defense and autophagy pathways triggered by blue light exposure in RPE cells., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
7. [Dynamics and Runoff Losses of Nitrogen in Paddy Field Surface Water Under Combined Application of Biochar and Slow/Controlled-Release Fertilizer].
- Author
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Si LL, Zhou JJ, Wu LH, and Hu ZP
- Subjects
- Agriculture, Ammonium Compounds analysis, Nitrates analysis, Oryza, Charcoal, Fertilizers, Nitrogen analysis, Water chemistry
- Abstract
Field experiments were conducted to investigate the effects of four fertilization treatments, that is, controlled-release fertilizer (CRF), biochar combined with controlled-release fertilizer (BC+ CRF), biochar combined with stabilized fertilizer (BC+ SF), and biochar combined with controlled-release fertilizer and stabilized fertilizer (BC+ CRF+ SF), on the dynamics of the pH and nitrogen mass concentration and runoff losses of nitrogen in paddy field surface water in the Taihu Lake Basin. The results show that the average pH of the surface water decreases by 3.16%-4.48% for BC+ CRF+ SF and is in the range of 5.64-8.15. The average total nitrogen (TN) mass concentration of surface water ranges from 19.05 to 25.23 mg·L
-1 . A significant decrease of 4.75%-6.58% in the TN mass concentration of surface water was observed for BC+ CRF+ SF. The average ammonium (NH4 + -N) and nitrate (NO3 - -N) mass concentration of the surface water vary from 0.01-17.26 mg·L-1 and from 0.24-3.11 mg·L-1 , respectively. Inorganic nitrogen is dominated by NH4 + -N in surface water. Compared with individual CRF, other treatments significantly reduce the NH4 + -N mass concentration of surface water by 35.89%-48.78% and the NO3 - -N mass concentration of surface water by 20.54%-37.01%. The BC+ SF combination shows a significant reduction in the NH4 + -N and NO3 - -N mass concentration of the surface water, which greatly lowers the risk of inorganic nitrogen loss via runoff. The runoff losses of TN, NH4 + -N, and NO3 - -N are in the range of 16.24-18.09, 1.76-2.22 and 0.76-1.38 kg·hm-2 , respectively. Compared with the individual CRF, the runoff loss of TN, NH4 + -N, and NO3 - -N of other treatments is reduced. The BC+ CRF+ SF combination shows a significant reduction in the runoff losses of nitrogen, which greatly lowers the risk of nitrogen nonpoint source pollution from paddy fields.- Published
- 2018
- Full Text
- View/download PDF
8. [Effect of agricultural application of municipal sewage sludge on plant-soil system: A review].
- Author
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Liu MJ, Xia SP, Wang J, Ma QX, Wang ZQ, and Wu LH
- Subjects
- Soil, Soil Pollutants, Agriculture, Metals, Heavy, Sewage
- Abstract
Currently, reasonable disposal of municipal sewage sludge is one of the important issues in the field of resources and environmental science. Sludge is rich in large amounts of organic matter and available nutrients, promoting soil fertility, soil physical structure and biological properties. However, sludge contains a variety of heavy metals, organic contaminants and other hazardous substance, especially heavy metals, which are the bottlenecks of agricultural application of sludge. To improve the sewage sludge utilization efficiency and decrease the effect on soil, this essay made a summary on domestic and foreign studies on plant-soil interaction ecosystem with sewage sludge to provide a theoretical basis and scientific guidance for advancing sewage sludge utilization efficiency.
- Published
- 2017
- Full Text
- View/download PDF
9. [Effects of soil pH on the competitive uptake of amino acids by maize and microorganisms].
- Author
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Ma QX, Wang J, Cao XC, Sun Y, Sun T, and Wu LH
- Subjects
- Fertilizers, Nitrogen, Plant Roots, Amino Acids, Soil, Zea mays
- Abstract
Organic nitrogen can play an important role in plant growth, and soil pH changed greatly due to the over-use of chemical fertilizers, but the effects of soil pH on the competitive uptake of amino acids by plants and rhizosphere microorganisms are lack of detailed research. To study the effects of soil pH on the uptake of amino acids by maize and soil microorganisms, two soils from Hangzhou and Tieling were selected, and the soil pH was changed by the electrokinesis, then the
15 N-labeled glycine was injected to the centrifuge tube with a short-term uptake of 4 h. Soil pH had a significant effect on the shoot and root biomass, and the optimal pH for maize shoot growth was 6.48 for Hangzhou red soil, while it was 7.65 for Tieling brown soil. For Hangzhou soil, the15 N abundance of maize shoots under pH=6.48 was significantly higher than under other treatments, and the uptake amount of15 N-glycine was also much higher. However, the15 N abundance of maize shoots and roots under pH=7.65 Tieling soil was significantly lower than it under pH=5.78, but the uptake amount of15 N-glycine under pH=7.65 was much higher. The microbial biomass C was much higher in pH=6.48 Hangzhou soil, while it was much lower in pH=7.65 Tieling soil. According to the results of root uptake, root to shoot transportation, and the competition with microorganisms, we suggested that although facing the fierce competition with microorganisms, the maize grown in pH=6.48 Hangzhou soil increased the uptake of glycine by increasing its root uptake and root to shoot transportation. While in pH=7.65 Tieling soil, the activity of microorganisms was decreased, which decreased the competition with maize for glycine, and increased the uptake of glycine by maize.- Published
- 2017
- Full Text
- View/download PDF
10. [Influence of a new phosphoramide urease inhibitor on urea-N transformation in different texture soil].
- Author
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Zhou X, Wu LH, and Dai F
- Subjects
- Aluminum Silicates, Ammonia, Clay, Nitrification, Nitrogen, Organophosphorus Compounds chemistry, Volatilization, Phosphoramides chemistry, Soil chemistry, Urea chemistry, Urease antagonists & inhibitors
- Abstract
Addition of urease inhibitors is one of the important measures to increase nitrogen (N) use efficiency of crop, due to retardant of urea hydrolysis and reduction of ammonia volatilization loss. An incubation experiment was conducted to investigate the urease inhibition effect of a new phosphoramide urease inhibitor, NPPT (N-(n-propyl) thiophosphoric triamide) in different texture soils under dark condition at 25 ℃, and NBPT (N-(n-butyl) thiophosphoric triamide) was obtained to compare the inhibition effect on urease in different soil textures by different dosages of urea adding. Results showed that the effective reaction time of urea was less than 9 d in the loamy and clay soil. Addition of inhibitors for retardation of urea hydrolysis was more than 3 d. In sandy soil, urea decomposition was relatively slow, and adding inhibitor significantly inhibited soil urease acti-vity, and reduced NH
4 + -N content. During the incubation time, the inhibition effect of high dosage urea in the soil was better than that of low dosage. At day 6, the urease inhibition rate of NBPT and NPPT (N 250 mg·kg-1 ) were 56.3% and 53.0% in sandy soil, 0.04% and 0.3% in loamy soil, 4.1% and 6.2% in clay soil; the urease inhibition rate of NBPT and NPPT (N 500 mg·kg-1 ) were 59.4% and 65.8% in sandy soil, 14.5% and 15.1% in loamy soil, 49.1% and 48.1% in clay soil. The urease inhibition effects in different texture soil were in order of sandy soil > clay soil> loamy soil. The soil NH4 + -N content by different inhibitors during incubation time increased at first and then decreased, while soil NO3 - -N content and apparent nitrification rate both showed rising trends. Compared with urea treatment, addition of urease inhibitors (NBPT and NPPT) significantly increased urea-N left in the soil and reduced NH4 + -N content. In short, new urease inhibitor NPPT in different texture is an effective urease inhibitor.- Published
- 2016
- Full Text
- View/download PDF
11. [Distribution characteristics of soil nitrogen and its influence factors in different typical zonal soils.]
- Author
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Cao XH, Zhong C, Ma QX, Zhu LF, Zhang JH, Yu SM, Jin QY, and Wu LH
- Subjects
- Adsorption, Amino Acids analysis, Hydrogen-Ion Concentration, Nitrates analysis, Nitrogen analysis, Soil chemistry
- Abstract
On the basis of field soil sampling, this paper investigated the distribution characteristics of soil different nitrogen (N) forms and its influence factors in the different typical zonal soils. The results showed that the concentrations of soil extractable total N, extractable organic N and adsorbed amino acids extracted with 0.5 mol·L
-1 K2 SO4 significantly increased along the altitudinal gradient in the different vertical soils, and their mean concentrations were greater than that in the horizontal soils. The concentrations of soil different N forms widely varied with the soil type in the different horizontal soils. On average, the concentration of soil adsorbed amino acids was approximately 5-fold greater than that of the free amino acids, representing 21.1% of soil extractable organic N. It indicated that the soil adsorbed amino acids extracted with the strong salt solution could serve as an important form of soil organic N. Pearson correlation analysis showed that extractable total N, extractable organic N, ammonium and amino acids in vertical soils were positively correlated with soil organic matter and total N (r=0.57-0.93, P<0.05), but negatively correlated with soil pH and nitrate (r=-0.37--0.91, P<0.05). In the horizontal soils, soil extractable total N, nitrate, organic matter, total N, alkali-hydrolyzable N and cation ions (e.g. K+ , Ca2+ , Mg2+ ) were all positively correlated with soil pH (r=0.36-0.85, P<0.05), whereas negatively correlated with soil ammonium and amino acids (r=-0.39--0.81, P<0.05).- Published
- 2016
- Full Text
- View/download PDF
12. [Advances in studies of absorption and utilization of amino acids by plants: A review].
- Author
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Cao XC, Wu LH, Ma QX, and Jin QY
- Subjects
- Carbon metabolism, Ecosystem, Nitrogen metabolism, Nitrogen Isotopes, Plant Roots, Soil Microbiology, Amino Acids metabolism, Plants metabolism, Soil chemistry
- Abstract
Plant can directly take up the intact amino acids, thus bypass the microbial mineralization of organic nitrogen. As an excellent carbon and nitrogen source, there exists competition for amino acid absorption between plant roots.and soil microorganisms. And the total flux of amino acids in soil may be enormous due to the extensive sources and short half-life. Studies on amino acid nitrogen nutritional contribution for plant by the technique of nitrogen isotopic tracer, has become a research topic in recent years ,which will help us better understand the principle of soil fertility. This paper summarized the recent researches on amino acid morphological characteristics in soil and its metabolic mechanism and nitrogen nutritional contribution for plant in different ecosystems, and discussed the present status and development trend of the amino acid circulation mechanism in the plant-soil-microorganism ecosystem and its bioavailability for plant. Finally, the topics of environmental regulating mechanism of amino acid bioavailability, amino acid carbon-nitrogen metabolism, and how to improve the field organic nitrogen management were all the core issues to be resolved.
- Published
- 2015
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