Search

Your search keyword '"Wingren, Anette Gjörloff"' showing total 28 results
Start Over Author "Wingren, Anette Gjörloff" Publication Year Range Last 10 years

Refine your results

more »

more »

more »
28 results on '"Wingren, Anette Gjörloff"'

4. Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior

Author

Yeung, Sing Yee, primary, Sergeeva, Yulia, additional, Pan, Guoqing, additional, Mittler, Silvia, additional, Ederth, Thomas, additional, Dam, Tommy, additional, Jönsson, Peter, additional, El-Schich, Zahra, additional, Wingren, Anette Gjörloff, additional, Tillo, Adam, additional, Hsiung Mattisson, Sabrina, additional, Holmqvist, Bo, additional, Stollenwerk, Maria M., additional, and Sellergren, Börje, additional

Published
2022
Full Text
View/download PDF

7. Circulating tumor cell models mimicking metastasizing cells in vitro : discrimination of colorectal cancer cells and white blood cells using digital holographic cytometry

Author

Feith, Marek, Zhang, Yuecheng, Persson, Jenny L., Balvan, Jan, El-Schich, Zahra, Wingren, Anette Gjörloff, Feith, Marek, Zhang, Yuecheng, Persson, Jenny L., Balvan, Jan, El-Schich, Zahra, and Wingren, Anette Gjörloff

Abstract

Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. In this study, we intended to mimic the environment for metastasizing cells. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers.

Published
2022
Full Text
View/download PDF

9. Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-Coated Polystyrene Cores—Assessment of the Binding Behavior to Cancer Cells

Author

Beyer, Sarah, primary, Kimani, Martha, additional, Zhang, Yuecheng, additional, Verhassel, Alejandra, additional, Sternbæk, Louise, additional, Wang, Tianyan, additional, Persson, Jenny L., additional, Härkönen, Pirkko, additional, Johansson, Emil, additional, Caraballo, Remi, additional, Elofsson, Mikael, additional, Gawlitza, Kornelia, additional, Rurack, Knut, additional, Ohlsson, Lars, additional, El-Schich, Zahra, additional, Wingren, Anette Gjörloff, additional, and Stollenwerk, Maria M., additional

Published
2022
Full Text
View/download PDF

10. Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-Coated Polystyrene Cores — Assessment of the Binding Behavior to Cancer Cells

Author

Beyer, Sarah, Kimani, Martha, Zhang, Yuecheng, Verhassel, Alejandra, Sternbæk, Louise, Wang, Tianyan, Persson, Jenny L., Härkönen, Pirkko, Johansson, Emil, Caraballo, Remi, Elofsson, Mikael, Gawlitza, Kornelia, Rurack, Knut, Ohlsson, Lars, El-Schich, Zahra, Wingren, Anette Gjörloff, and Stollenwerk, Maria M.

Subjects
sialic acid, Cell- och molekylärbiologi, Biochemistry and Molecular Biology, SA conjugates, cancer, molecularly imprinted polymers, imprinting, Biokemi och molekylärbiologi, Cell and Molecular Biology
Abstract

Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Lectins are widely used to analyze the cell surface expression of SA. However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. In this study, SA-imprinted fluorescent core-shell molecularly imprinted polymer particles (SA-MIPs) were employed to recognize SA on the cell surface of cancer cell lines. The SA-MIPs improved suspensibility and scattering properties compared with previously used core-shell SA-MIPs. Although SA-imprinting was performed using SA without preference for the α2,3- and α2,6-SA forms, we screened the cancer cell lines analyzed using the lectins Maackia Amurensis Lectin I (MAL I, α2,3-SA) and Sambucus Nigra Lectin (SNA, α2,6-SA). Our results show that the selected cancer cell lines in this study presented a varied binding behavior with the SA-MIPs. The binding pattern of the lectins was also demonstrated. Moreover, two different pentavalent SA conjugates were used to inhibit the binding of the SA-MIPs to breast, skin, and lung cancer cell lines, demonstrating the specificity of the SA-MIPs in both flow cytometry and confocal fluorescence microscopy. We concluded that the synthesized SA-MIPs might be a powerful future tool in the diagnostic analysis of various cancer cells.

Published
2022

11. FcγRIIIa receptor interacts with androgen receptor and PIP5K1α to promote growth and metastasis of prostate cancer

Author

Larsson, Per Flodbring, primary, Karlsson, Richard, additional, Sarwar, Martuza, additional, Miftakhova, Regina, additional, Wang, Tianyan, additional, Syed Khaja, Azharuddin Sajid, additional, Semenas, Julius, additional, Chen, Sa, additional, Hedblom, Andreas, additional, Ali, Amjad, additional, Ekström‐Holka, Kristina, additional, Simoulis, Athanasios, additional, Kumar, Anjani, additional, Wingren, Anette Gjörloff, additional, Robinson, Brian, additional, Nyunt Wai, Sun, additional, Mongan, Nigel P., additional, Heery, David M., additional, Öhlund, Daniel, additional, Grundström, Thomas, additional, Ødum, Niels, additional, and Persson, Jenny L., additional

Published
2022
Full Text
View/download PDF

12. Sialic Acid as a Biomarker Studied in Breast Cancer Cell Lines In Vitro Using Fluorescent Molecularly Imprinted Polymers

Author

El-Schich, Zahra, Zhang, Yuecheng, Göransson, Tommy, Dizeyi, Nishtman, Persson, Jenny L., Johansson, Emil, Caraballo, Remi, Elofsson, Mikael, Shinde, Sudhirkumar, Sellergren, Börje, and Wingren, Anette Gjörloff

Subjects
lcsh:T, Cell- och molekylärbiologi, lcsh:Technology, Sialic acid, lcsh:QC1-999, Analytical Chemistry, lcsh:Chemistry, Breast cancer, breast cancer, Epithelial cell adhesion molecule, lcsh:Biology (General), lcsh:QD1-999, sialic acid, lcsh:TA1-2040, Molecularly imprinted polymers, Analytisk kemi, Nanoparticles, molecularly imprinted polymers, nanoparticles, epithelial cell adhesion molecule, lcsh:Engineering (General). Civil engineering (General), lcsh:QH301-705.5, Cell and Molecular Biology, lcsh:Physics
Abstract

Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation, and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors, correlating with poor prognosis. To overcome the lack of natural SA-receptors, such as antibodies and lectins with high enough specificity and sensitivity, we have used molecularly imprinted polymers (MIPs), or “plastic antibodies”, as nanoprobes. Because high expression of epithelial cell adhesion molecule (EpCAM) in primary tumors is often associated with proliferation and a more aggressive phenotype, the expression of EpCAM and CD44 was initially analyzed. The SA-MIPs were used for the detection of SA on the cell surface of breast cancer cells. Lectins that specifically bind to the a-2,3 SA and a-2,6 SA variants were used for analysis of SA expression, with both flow cytometry and confocal microscopy. Here we show a correlation of EpCAM and SA expression when using the SA-MIPs for detection of SA. We also demonstrate the binding pattern of the SA-MIPs on the breast cancer cell lines using confocal microscopy. Pre-incubation of the SA-MIPs with SA-derivatives as inhibitors could reduce the binding of the SA-MIPs to the tumor cells, indicating the specificity of the SA-MIPs. In conclusion, the SA-MIPs may be a new powerful tool in the diagnostic analysis of breast cancer cells.

Published
2021

13. Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements : Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

Author

El-Schich, Zahra, Janicke, Birgit, Alm, Kersti, Dizeyi, Nishtman, Persson, Jenny L., Wingren, Anette Gjörloff, El-Schich, Zahra, Janicke, Birgit, Alm, Kersti, Dizeyi, Nishtman, Persson, Jenny L., and Wingren, Anette Gjörloff

Abstract

Breast cancer is the second most common cancer worldwide. Metastasis is the main reason for death in breast cancer, and today, there is a lack of methods to detect and isolate circulating tumor cells (CTCs), mainly due to their heterogeneity and rarity. There are some systems that are designed to detect rare epithelial cancer cells in whole blood based on the most common marker used today, the epithelial cell adhesion molecule (EpCAM). It has been shown that aggressive breast cancer metastases are of non-epithelial origin and are therefore not always detected using EpCAM as a marker. In the present study, we used an in vitro-based circulating tumor cell model comprising a collection of six breast cancer cell lines and white blood cell lines. We used digital holographic cytometry (DHC) to characterize and distinguish between the different cell types by area, volume and thickness. Here, we present significant differences in cell size-related parameters observed when comparing white blood cells and breast cancer cells by using DHC. In conclusion, DHC can be a powerful diagnostic tool for the characterization of CTCs in the blood.

Published
2020
Full Text
View/download PDF

15. Sialic acid as a biomarker studied in breast cancer cells using fluorescent molecularly imprinted polymers, MIPs

Author

El-Schich, Zahra, primary, Zhang, Yuecheng, additional, Göransson, Tommy, additional, Dizeyi, Nishtman, additional, Persson, Jenny, additional, Johansson, Emil, additional, Caraballo, Remi, additional, Elofsson, Mikael, additional, Shinde, Sudhirkumar, additional, Sellergren, Börje, additional, and Wingren, Anette Gjörloff, additional

Published
2020
Full Text
View/download PDF

20. Supervised classification of etoposide-treated in vitro adherent cells based on noninvasive imaging morphology

Author

Mölder, Anna Leida, Persson, Johan, El-Schich, Zahra, Czanner, Silvester, Wingren, Anette Gjörloff, Mölder, Anna Leida, Persson, Johan, El-Schich, Zahra, Czanner, Silvester, and Wingren, Anette Gjörloff

Abstract

Single-cell studies using noninvasive imaging is a challenging, yet appealing way to study cellular characteristics over extended periods of time, for instance to follow cell interactions and the behavior of different cell types within the same sample. In some cases, e.g., transplantation culturing, real-time cellular monitoring, stem cell studies, in vivo studies, and embryo growth studies, it is also crucial to keep the sample intact and invasive imaging using fluorophores or dyes is not an option. Computerized methods are needed to improve throughput of image-based analysis and for use with noninvasive microscopy such methods are poorly developed. By combining a set of well-documented image analysis and classification tools with noninvasive microscopy, we demonstrate the ability for long-term image-based analysis of morphological changes in single cells as induced by a toxin, and show how these changes can be used to indicate changes in biological function. In this study, adherent cell cultures of DU-145 treated with low-concentration (LC) etoposide were imaged during 3 days. Single cells were identified by image segmentation and subsequently classified on image features, extracted for each cell. In parallel with image analysis, an MTS assay was performed to allow comparison between metabolic activity and morphological changes after long-term low-level drug response. Results show a decrease in proliferation rate for LC etoposide, accompanied by changes in cell morphology, primarily leading to an increase in cell area and textural changes. It is shown that changes detected by image analysis are already visible on day 1 for [Formula: see text] etoposide, whereas effects on MTS and viability are detected only on day 3 for [Formula: see text] etoposide concentration, leading to the conclusion that the morphological changes observed occur before and at lower concentrations than a reduction in cell metabolic activity or viability. Three classifiers are compared and we

Published
2017
Full Text
View/download PDF

22. Targeted suppression of AR-V7 using PIP5K1α inhibitor overcomes enzalutamide resistance in prostate cancer cells

Author

Sarwar, Martuza, primary, Semenas, Julius, additional, Miftakhova, Regina, additional, Simoulis, Athanasios, additional, Robinson, Brian, additional, Wingren, Anette Gjörloff, additional, Mongan, Nigel P., additional, Heery, David M., additional, Johnsson, Heather, additional, Abrahamsson, Per-Anders, additional, Dizeyi, Nishtman, additional, Luo, Jun, additional, and Persson, Jenny L., additional

Published
2016
Full Text
View/download PDF

28. An Epitope-Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell-Biomaterial Interactions.

Author

Pan G, Shinde S, Yeung SY, Jakštaitė M, Li Q, Wingren AG, and Sellergren B

Subjects
3T3 Cells, Animals, Cell Communication, Ligands, Mice, Molecular Structure, Surface Properties, Biocompatible Materials chemistry, Epitopes chemistry, Fibroblasts chemistry, Molecular Imprinting, Oligopeptides chemistry
Abstract

In this study, an epitope-imprinting strategy was employed for the dynamic display of bioactive ligands on a material interface. An imprinted surface was initially designed to exhibit specific affinity towards a short peptide (i.e., the epitope). This surface was subsequently used to anchor an epitope-tagged cell-adhesive peptide ligand (RGD: Arg-Gly-Asp). Owing to reversible epitope-binding affinity, ligand presentation and thereby cell adhesion could be controlled. As compared to current strategies for the fabrication of dynamic biointerfaces, for example, through reversible covalent or host-guest interactions, such a molecularly tunable dynamic system based on a surface-imprinting process may unlock new applications in in situ cell biology, diagnostics, and regenerative medicine., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results