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In E. coli, NusG is an essential regulator of RNA polymerase. Due to its two domain structure, NusG acts as flexible linker in the anti termination complex which promotes elongation rates by suppressing transcriptional pausing in vitro. However the specific role of NusG in the process of N antitermination is still unclear as antitermination has been observed in the absence of NusG, in vivo. Here we attempt to resolve this issue by isolating a point mutation in NusG that blocks N antitermination. Doing so will reveal the structural interaction between the Nus G and N protein during phage Lambda infection. [ABSTRACT FROM AUTHOR]

This article will review our current understanding of transcription elongation and termination in E. coli. We discuss why transcription elongation complexes pause at certain template sites and how auxiliary host and phage transcription factors affect elongation and termination. The connection between translation and transcription elongation is described. Finally we present an overview indicating where progress has been made and where it has not. [ABSTRACT FROM AUTHOR]