26 results on '"Traore Hamidou"'
Search Results
2. Screening for Resistance to Striga Hermonthica in Mutagenized Sorghum and Upland Rice in Burkina Faso
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Yonli, Djibril, Nikiéma, Philippe M., Traoré, Hamidou, Ghanim, Abdelbagi M. A., Ghanim, Abdelbagi M. A., editor, Sivasankar, Shoba, editor, and Rich, Patrick J., editor
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- 2024
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3. Potentiels évoqués cognitifs en psychiatrie : expérience de terrain
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Traore, Hamidou, Borde, Stanley, Blanchard-Bouhajja, Françoise, Rocher, Joëlle, Girard, Murielle, and Lacroix, Aurélie
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- 2024
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4. Opportunities and Challenges for Decentralized Clinical Trial Approaches: European Health Technology Assessment Perspective
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de Jong, Amos J., Shahid, Nadi, Zuidgeest, Mira G.P., Santa-Ana-Tellez, Yared, Hogervorst, Milou, Goettsch, Wim, Traore, Hamidou, de Boer, Anthonius, and Gardarsdottir, Helga
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- 2024
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5. Evaluation of granular formulated strigolactone analogs for Striga suicidal germination.
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Jamil, Muhammad, Margueritte, Ouedraogo, Yonli, Djibril, Wang, Jian You, Navangi, Lynet, Mudavadi, Patrick, Patil, Rohit H., Bhoge, Satish Ekanath, Traore, Hamidou, Runo, Steven, and Al‐Babili, Salim
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PURPLE witchweed ,SOIL seed banks ,WITCHWEEDS ,AGRICULTURE ,GERMINATION - Abstract
BACKGROUND: Striga hermonthica, an obligate root parasitic weed, poses a significant threat to cereal production in sub‐Saharan Africa. Lowering Striga seed bank in infested soils is a promising strategy to mitigate infestation levels. The dependency of Striga seed germination on strigolactones opens up the possibility of a 'suicidal germination' approach, where synthetic germination stimulants induce lethal germination in the absence of a host. Implementing this approach requires active germination stimulants with a suitable formulation for field application. Here, we describe the development of slow‐releasing granular formulation of two potent germination stimulants 'Methyl Phenlactonoate 3' and 'Nijmegen‐1' and the assessment of their activity under Laboratory, greenhouse, mini‐field, and field conditions. RESULTS: Under laboratory conditions, the granular formulation of either of the two germination stimulants (1.25 mg per plate, corresponding to 0.09 mg a.i.) induced Striga seed germination at a rate of up to 43%. With 10 mg granular product (0.75 mg a.i.) per pot, we observed 77–83% reduction in Striga emergence under greenhouse pot conditions. Application of the formulated stimulants under artificially or naturally infested fields resulted in approximately 56%, 60%, and 72% reduction in Striga emergence in maize, sorghum, and millet fields in Kenya and Burkina Faso, respectively. CONCLUSION: Our findings on the newly designed granular formulation of Methyl Phenlactonoate 3 and Nijmegen‐1 reveal encouraging prospects for addressing the Striga problem in Africa. These findings underscore several significant advantages of the formulated stimulants, including suitability for the African agricultural context, and, most importantly, their effectiveness in reducing Striga infection. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Opportunities and Challenges for Decentralized Clinical Trial Approaches: European Health Technology Assessment Perspective
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de Jong, Amos J., primary, Shahid, Nadi, additional, Zuidgeest, Mira G.P., additional, Santa-Ana-Tellez, Yared, additional, Hogervorst, Milou, additional, Goettsch, Wim, additional, Traore, Hamidou, additional, de Boer, Anthonius, additional, and Gardarsdottir, Helga, additional
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- 2023
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7. Effets du travail du sol et de la fertilisation organo-minérale sur l’humidité du sol et l’assimilation chlorophyllienne du sorgho en culture associée avec le niébé
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Koumbem, Mahamoudou, Pale, Siébou, Hien, Edmond, Yonli, Djibril, Traore, Hamidou, Pale, Grégoire, Prasad, P. V. Vara, and Middendorf, B. Jan
- Abstract
La précarité des agroécosystèmes des pays sahéliens impose aux producteurs des options de gestion intégrée de la fertilité des sols. Cette étude avait pour objectif d’évaluer les effets du travail du sol et du système de culture avec fertilisation sur l’état hydrique du sol et le taux de chlorophylle des feuilles du sorgho associé avec le niébé. Le dispositif expérimental était un bloc complètement randomisé avec les traitements arrangés en split plot, quatre méthodes de travail du sol comme parcelles principales, seize systèmes de culture avec fertilisation comme parcelles secondaires et trois répétitions. Le zaï manuel a permis d’obtenir des humidités du sol à 90 jours Après Semis plus élevées avec des taux variant de 78,57 à 263,64% par rapport au labour, au travail minimum du sol et au billonnage cloisonné. Les taux de chlorophylle les plus élevés ont été enregistrés au stade montaison dans les parcelles labourées et de billonnage cloisonné, également avec le système de culture comprenant 1 ligne de sorgho + 1 ligne de niébé à port rampant recevant des engrais NPK et urée. Ces résultats montrent que le travail du sol combiné à la fertilisation organo-minérale est susceptible d’optimiser la productivité agricole. English title: Effects of tillage and organo-mineral fertilization on soil moisture and chlorophyll assimilation of sorghum intercropped with cowpea The precariousness of the agro-ecosystems in the sahelian countries is forcing farmers to adopt diverseoptions of integrated soil fertility management. This study carried out at the Saria environmental andagricultural research Station in 2020 and 2021 aimed to assess the effects of tillage and cropping system with fertilization on the water status of the soil and the chlorophyll level in the leaves of sorghum intercropped with cowpea. The experimental design was a completely randomized block with treatments arranged in a split plot, four tillage methods as main plots, sixteen cropping systems with fertilization subplots and three replications. The manual zaï made it possible to obtain higher soil humidity at 90 days after planting with rates varying from 78.57 to 263.64% compared to ploughing, minimum tillage and tied-ridging. The highest chlorophyll levels were recorded at boot stage in the ploughed and tied-ridging plots, also with the cropping system of 1 line of sorghum alternated with 1 line of cowpea cropping system and receiving NPK and urea fertilizers. These results show that tillage method combined with organo-mineral fertilization is likely to optimize agricultural productivity. 
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- 2023
8. Management of Omphalocele at the Regional Hospital Fousseyni Daou of Kayes, Mali
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Kouyaté Mamaye, Lamine Issaga Traore, Coulibaly Mamadou Bernard, Toure Sidy Mohama, Dembélé Singoun, Dicko Batté, kané Moustapha, Traore Hamidou, and Coulibaly Yacaridia
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General Engineering - Abstract
Introduction: the omphalocele is defined as a congenital malformation of the abdominal wall from the umbilical ring leading to non-integration of the viscera covered by an amniotic and avascular membrane. The cord still fits over the membrane. There are sometimes cavities between the viscera and the membrane that contain wharton jelly. The frequency is 1 case in 6000 births in France and 1 case in 5000 in Germany. We conducted this study in order to describe the epidemiological aspects and to evaluate the therapeutic results of omphaloceles in the regional hospital of Kayes. Materials and methods: We conducted a 15-month retrospective study from January 2020 to March 2021 in the pediatric surgery unit of the Kayes regional hospital, all newborns (0 to 6 days old) admitted to the ward with omphalocele were included. in the study. Other newborns with a malformation in addition to laparochisis were excluded from the study. Results: 11 patients were recorded in the study including 7 boys and 4 girls. The average age was 3.5 days and the average weight was 3 kg 350. According to the classification of Aitken 7 were type II and 4 cases type I. The technique of primary musculo-aponeurotic closure was used in all cases. Intraoperative hepatic hemorrhage occurred in 2 cases. We had recorded 2 deaths. No mother had a history of taking teratogenic drugs, 7 were multiparts. Ultrasound had found omphaloceles in 3 antenatal patients. Conclusion: Omphaloceles is a surgical emergency for which the management is multidisciplinary and requires an antenatal ultrasound.
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- 2021
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9. A search to reducing Striga hermonthica (Del.) Benth propagation using plant aqueous extracts with bio-herbicide properties on the seeds in vitro
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SAWADOGO-ILBOUDO, Tinkoudougou Cathérine, primary, YONLI, Djibril, additional, SOURABIE, Soumaïla, additional, ZERBO, Patrice, additional, TRAORE, Hamidou, additional, and BOUSSIM, Joseph Issaka, additional
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- 2022
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10. Striga hermonthica Suicidal Germination Activity of Potent Strigolactone Analogs: Evaluation from Laboratory Bioassays to Field Trials
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Jamil, Muhammad, primary, Wang, Jian You, additional, Yonli, Djibril, additional, Ota, Tsuyoshi, additional, Berqdar, Lamis, additional, Traore, Hamidou, additional, Margueritte, Ouedraogo, additional, Zwanenburg, Binne, additional, Asami, Tadao, additional, and Al-Babili, Salim, additional
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- 2022
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11. A New Formulation for Strigolactone Suicidal Germination Agents, towards Successful Striga Management
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Jamil, Muhammad, primary, Wang, Jian You, additional, Yonli, Djibril, additional, Patil, Rohit H., additional, Riyazaddin, Mohammed, additional, Gangashetty, Prakash, additional, Berqdar, Lamis, additional, Chen, Guan-Ting Erica, additional, Traore, Hamidou, additional, Margueritte, Ouedraogo, additional, Zwanenburg, Binne, additional, Bhoge, Satish Ekanath, additional, and Al-Babili, Salim, additional
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- 2022
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12. Inventaire des mauvaises herbes et des méthodes de lutte contre l’enherbement dans les exploitations de la zone cotonnière Est du Burkina Faso
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Etiabi, Bétia, primary, Koulibaly, Bazoumana, additional, and Traore, Hamidou, additional
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- 2021
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13. Potentiels évoqués cognitifs en psychiatrie : expérience de terrain
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Traore, Hamidou, primary, Borde, Stanley, additional, Blanchard-Bouhajja, Françoise, additional, Rocher, Joëlle, additional, Girard, Murielle, additional, and Lacroix, Aurélie, additional
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- 2021
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14. Typology of Farms and Farmers’ Perception of the Effects of Soil and Water Conservation Practices in Northern Burkina Faso
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Noufe Tiatite, Traore Hamidou, Mason C. Stephen, Yonli Djibril, Fofana Souleymane, Prasad P. V. Vara, Pale Siebou, Zachary P. Stewart, and Coulibaly Zoumité Christ Thierry Stephen
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Typology ,Geography ,Agroforestry ,Perception ,media_common.quotation_subject ,Soil Science ,Animal Science and Zoology ,Forestry ,Plant Science ,Soil conservation ,Agronomy and Crop Science ,Food Science ,media_common - Abstract
The continuous degradation of agroecosystem is a major concern for Sub-Saharan African countries, particularly Burkina Faso. To fight against this agroecosystem degradation, SWC such as stone rows, grass strips, zaï, filtering dikes, half-moons and agroforestry had been introduced in the Yatenga Province in Northern Burkina Faso several decades ago. Decades after introduction of SWC practices, a survey was conducted with 120 farmers equally distributed in four villages in the region to learn the farmers’ perceptions of the effects of these practices on their farms. Results revealed a higher proportion of men in the study sites (63%) compared to women. The largest difference in proportions between surveyed men and women was observed in Bogoya where only 22% of the surveyed persons were women. The average years of the respondents across villages was 53 years with 57% of farmers being members of at least one farmer organization. The proportion of educated farmers was 73% and those who received training in SWC techniques represented 36%. Results indicated that white grain sorghum and pearl millet were the main crops produced by 95% of farmers and stone rows and zaï were the dominant SWC techniques used by 77-80% of farmers. Data from the survey indicated a fairly high proportion of big ruminant breeders and small ruminant breeders as well. In fact, 79% of farmers bred big ruminants and 78% bred small ruminants. The main beneficial and direct effects of SWC techniques perceived by farmers was their capacity to improve soil fertility, recover soil, reduce water run-off, and allow good water infiltration in the field, thus improving soil productivity. Farmers pointed out indirect effects of SWC practices on livestock by the regeneration and increase of grass, tree and small shrub biomass available to improve animal growth and health. However, some farmers commented that the long-term use of zaï could lead to soil degradation. The study showed that farmers did perceive the beneficial effects of SWC practices and that greater extension and adoption will only be achieved if they could still receive training, financial and equipment supports.
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- 2019
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15. Effets du travail du sol et de la fertilisation organo-minérale sur les rendements du mil en zone soudano-sahélienne du Burkina Faso
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Pale, Siébou, primary, Barro, Albert, additional, Koumbem, Mahamoudou, additional, Sere, Aïda, additional, and Traore, Hamidou, additional
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- 2021
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16. Mauvaises herbes majeures de la culture de haricot (Phaseolus vulgaris L.) dans la Région du Moronou au Centre-Est de la Côte d’Ivoire
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Ahonon, Brou Alain, Traore, Hamidou, and Ipou, Joseph Ipou
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Haricot, mauvaises herbes, espèces nuisibles, Côte d’Ivoire - Abstract
Le haricot (Phaseolus vulgaris L.) constitue une source de compléments protéiques pour l’alimentation des populations. Cependant, les mauvaises herbes affectent la productivité de cette légumineuse. Cette étude a pour objectif de caractériser la flore adventice dans les parcelles de haricot. Des inventaires floristiques et des analyses phytosociologiques ont été conduits en 2014 et en 2015 dans des parcelles de haricot localisées dans trois départements de la Côte d’Ivoire. Ainsi, 190 relevés floristiques ont été réalisés permettant de recenser 229 espèces adventices réparties en 157 genres appartenant à 57 familles. Les familles les mieux représentées sont par ordre d’importance les Poaceae (11,35%), les Fabaceae (7,86%), les Asteraceae (7,42%), les Euphorbiaceae (6,97%), les Cyperaceae (3,93%), les Caesalpiniaceae (3,93%) et les Amaranthaceae (3,49%). L’analyse de la fréquence et de l’abondance-dominance des espèces a révélé que Chromolaena odorata (L.) R. M. King et H. Rob. et Croton hirtus l’Hérit. sont les adventices les plus nuisibles dans les parcelles de haricot.Mots clés: Haricot, mauvaises herbes, espèces nuisibles, Côte d’Ivoire.
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- 2018
17. Economic Performance of Soil and Water Conservation Practicesin Burkina Faso
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Noufe Tiatite, Mason C. Stephen, Yonli Djibril, Zachary P. Stewart, Pale Siebou, Coulibaly Zoumité Christ Thierry Stephen, Fofana Souleymane, Prasad P. V. Vara, and Traore Hamidou
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Agroecosystem ,biology ,Agroforestry ,Yield (finance) ,0207 environmental engineering ,02 engineering and technology ,010501 environmental sciences ,Sorghum ,biology.organism_classification ,Investment (macroeconomics) ,01 natural sciences ,Stochastic frontier analysis ,Geography ,Sustainability ,Immunology and Allergy ,Agricultural productivity ,020701 environmental engineering ,Soil conservation ,0105 earth and related environmental sciences - Abstract
The continuous degradation of agroecosystems is a major concern for Sub-Saharan African countries, particularly Burkina Faso. To fight against this problem, various research projects and programs have implemented S oil and W ater C onservation practices (SWC) in Northern Burkina Faso. The objective of this study was to assess the economic performance of stone rows, grass strips, zai, filtering dikes, half-moons and agroforestry on agricultural production in this part of Burkina Faso. Stochastic Frontier Analysis was used to estimate SWC’s technical efficiency. Results indicated that the cost for SWC construction did not influence white sorghum and pearl millet yield. However, an increase of 1% in the investment for SWC implementation results in a 0.42% increase in groundnut yield and 0.19% in cowpea yield. Although, the half-moon technique had a positive effect on the farmer’s technical efficiency, the effects of stone rows, filtering dikes, zai and grass strips were not significant. Given the tremendous efforts that farmers develop to implement these anti-erosion practices, one recommendation is that policy makers strengthen the technical, financial and equipment supports to farmers for efficient implementation of SWC techniques to ensure sustainability of agricultural production systems in Northern Burkina Faso.
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- 2019
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18. Acquired resistance to macrolides inPseudomonas aeruginosafrom cystic fibrosis patients
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UCL - SSS/LDRI - Louvain Drug Research Institute, Mustafa, Muhammad Hariri, Khandekar, Shaunak, Tunney, Michael M., Elborn, Stuart J., Kahl, Barbara C., Denis, Olivier, Plésiat, Patrick, Traore, Hamidou, Tulkens, Paul M., Vanderbist, Francis, Van Bambeke, Françoise, UCL - SSS/LDRI - Louvain Drug Research Institute, Mustafa, Muhammad Hariri, Khandekar, Shaunak, Tunney, Michael M., Elborn, Stuart J., Kahl, Barbara C., Denis, Olivier, Plésiat, Patrick, Traore, Hamidou, Tulkens, Paul M., Vanderbist, Francis, and Van Bambeke, Françoise
- Abstract
Cystic fibrosis (CF) patients receive chronic treatment with macrolides for their antivirulence and anti-inflammatory properties. We, however, previously showed that Pseudomonas aeruginosa, considered as naturally resistant to macrolides, becomes susceptible when tested in a eukaryotic medium rather than a conventional broth. We therefore looked for specific macrolide resistance determinants in 333 CF isolates from four European CF centres in comparison with 48 isolates from patients suffering from hospital-acquired pneumonia (HAP). Minimum inhibitory concentrations (MICs) of macrolides and ketolides measured in eukaryotic medium (RPMI-1640) were higher towards CF than HAP isolates. Gene sequencing revealed mutations at three positions (2045, 2046 and 2598) in domain V of 23S rRNA of 43% of sequenced CF isolates, but none in HAP isolates. Enzymes degrading extracellular polymeric substances also reduced MICs, highlighting a role of the mucoid, biofilm-forming phenotype in resistance. An association between high MICs and chronic azithromycin administration was evidenced, which was statistically significant for patients infected by the Liverpool Epidemic Strain. Thus, ribosomal mutations are highly prevalent in CF isolates and may spread in epidemic clones, arguing for prudent use of oral macrolides in these patients. Measuring MICs in RPMI-1640 could be easily implemented in microbiology laboratories to phenotypically detect resistance.
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- 2017
19. Farmers' perception on impact of drought and their preference for sorghum cultivars in Burkina Faso
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Ouedraogo, Nofou, Sanou, Jacob, Kam, Honore, Traore, Hamidou, Adam, Myriam, Gracen, Vernon, Danquah, Eric Y., Ouedraogo, Nofou, Sanou, Jacob, Kam, Honore, Traore, Hamidou, Adam, Myriam, Gracen, Vernon, and Danquah, Eric Y.
- Abstract
Sorghum production in Burkina Faso relies on small scale farmers. Unfortunately, many suffer from drought caused by erratic rainfall during the rainy season. The importance of understanding farmers' perception on impact of drought and different ways to manage it is necessary. Participatory rural approach (PRA) using a focus group discussion and semi-structured interviews with 340 farmers was conducted in six districts located in two agro ecological zones. The study investigated famers' perceptions on sorghum production constraints with emphasis on drought impact, drought management, sorghum varieties farmers grow and their traits of choice. Results showed Striga (Striga hermontica) is the major constraint limiting sorghum production. Drought was the second factor reducing sorghum yield and was the most important abiotic constraint. Farmers showed a good empirical knowledge about drought and indicated that post-flowering drought was recurrent but less damaging than other types of drought. To alleviate drought effect, farmers employed different methods including cultural practices, soil and water conservation, use early maturity materials and prayers for rain. Until now, farmers rely more on their local landraces than improved varieties for sorghum production and want high yielding varieties that withstand drought, have white grain color and are high edible.
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- 2017
20. Acquired resistance to macrolides in Pseudomonas aeruginosa from cystic fibrosis patients
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Mustafa, Muhammad-Hariri, Vanderbist, Francis, Van Bambeke, Françoise, Khandekar, Shaunak, Tunney, Michael, Elborn, Stuart J.S., Kahl, Barbara C, Denis, Olivier, Plésiat, Patrick, Traore, Hamidou, Tulkens, Paul M., Mustafa, Muhammad-Hariri, Vanderbist, Francis, Van Bambeke, Françoise, Khandekar, Shaunak, Tunney, Michael, Elborn, Stuart J.S., Kahl, Barbara C, Denis, Olivier, Plésiat, Patrick, Traore, Hamidou, and Tulkens, Paul M.
- Abstract
Cystic fibrosis (CF) patients receive chronic treatment with macrolides for their antivirulence and anti-inflammatory properties. We, however, previously showed that Pseudomonas aeruginosa, considered as naturally resistant to macrolides, becomes susceptible when tested in a eukaryotic medium rather than a conventional broth. We therefore looked for specific macrolide resistance determinants in 333 CF isolates from four European CF centres in comparison with 48 isolates from patients suffering from hospital-acquired pneumonia (HAP). Minimum inhibitory concentrations (MICs) of macrolides and ketolides measured in eukaryotic medium (RPMI-1640) were higher towards CF than HAP isolates. Gene sequencing revealed mutations at three positions (2045, 2046 and 2598) in domain V of 23S rRNA of 43% of sequenced CF isolates, but none in HAP isolates. Enzymes degrading extracellular polymeric substances also reduced MICs, highlighting a role of the mucoid, biofilm-forming phenotype in resistance. An association between high MICs and chronic azithromycin administration was evidenced, which was statistically significant for patients infected by the Liverpool Epidemic Strain. Thus, ribosomal mutations are highly prevalent in CF isolates and may spread in epidemic clones, arguing for prudent use of oral macrolides in these patients. Measuring MICs in RPMI-1640 could be easily implemented in microbiology laboratories to phenotypically detect resistance., SCOPUS: ar.j, info:eu-repo/semantics/published
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- 2017
21. Genetic diversity among sorghum landraces and polymorphism assessment of local improved varieties for stay-green trait
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Ouedraogo, Nofou, primary, Sanou, Jacob, additional, Traore, Hamidou, additional, Gracen, Vernon, additional, Tongoona, Pangirayi, additional, and Danquah, Eric Y., additional
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- 2017
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22. Acquired resistance to macrolides inPseudomonas aeruginosafrom cystic fibrosis patients
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Mustafa, Muhammad-Hariri, primary, Khandekar, Shaunak, additional, Tunney, Michael M., additional, Elborn, J. Stuart, additional, Kahl, Barbara C., additional, Denis, Olivier, additional, Plésiat, Patrick, additional, Traore, Hamidou, additional, Tulkens, Paul M., additional, Vanderbist, Francis, additional, and Van Bambeke, Françoise, additional
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- 2017
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23. Antimicrobial susceptibility of Pseudomonas aeruginosa isolated from cystic fibrosis patients through Northern Europe.
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Mustafa, Muhammad-Hariri, Chalhoub, Hussein, Denis, Olivier, Deplano, Ariane, Vergison, Anne, Rodriguez Villalobos, Hector, Tunney, Michael, Elborn, Joseph Stuart, Kahl, Barbara C, Traore, Hamidou, Vanderbist, Francis, Tulkens, Paul M., Van Bambeke, Françoise, Mustafa, Muhammad-Hariri, Chalhoub, Hussein, Denis, Olivier, Deplano, Ariane, Vergison, Anne, Rodriguez Villalobos, Hector, Tunney, Michael, Elborn, Joseph Stuart, Kahl, Barbara C, Traore, Hamidou, Vanderbist, Francis, Tulkens, Paul M., and Van Bambeke, Françoise
- Abstract
Pseudomonas aeruginosa is a major cause of morbidity and mortality in cystic fibrosis patients. This study compares the antimicrobial susceptibility of 153 P. aeruginosa isolates from the United Kingdom (UK) (n=58), Belgium (n=44), and Germany (n=51) collected from 120 patients during routine visits over the 2006-2012 period. MICs were measured by broth microdilution. Genes encoding extended spectrum β-lactamases (ESBL), metallo-β-lactamases and carbapenemases were detected by PCR. Pulsed Field Gel Electrophoresis and Multi-Locus Sequence Typing were performed on isolates resistant to ≥ 3 antibiotic classes among penicillins/cephalosporins, carbapenems, fluoroquinolones, aminoglycosides, polymyxins. Based on EUCAST/CLSI breakpoints, susceptibility was ≤ 30%/≤ 40% (penicillins, ceftazidime, amikacin, ciprofloxacin), 44-48%/48-63% (carbapenems), 72%/72% (tobramycin), and 92%/78% (colistin) independently of patient's age. Sixty percent of strains were multidrug resistant (MDR; European Centre for Disease prevention and Control criteria). Genes encoding ESBL (most prevalent BEL, PER, GES, VEB, CTX-M, TEM, SHV, and OXA), metallo β-lactamases (VIM, IMP, NDM), or carbapenemases (OXA-48, KPC) were not detected. The Liverpool Epidemic Strain (LES) was prevalent in UK isolates only (75% of MDR isolates). Four MDR ST958 isolates were found spread over the three countries. The other MDR clones were evidenced in ≤ 3 isolates and localized in a single country. A new sequence type (ST2254) was discovered in one MDR isolate in Germany. Clonal and non-clonal isolates with different susceptibility profiles were found in 21 patients. Thus, resistance and MDR are highly prevalent in routine isolates from 3 countries, with carbapenem (meropenem), tobramycin and colistin remaining the most active drugs., SCOPUS: ar.j, info:eu-repo/semantics/published
- Published
- 2016
24. Antimicrobial Susceptibility of Pseudomonas aeruginosa Isolated from Cystic Fibrosis Patients in Northern Europe
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Mustafa, Muhammad-Hariri, primary, Chalhoub, Hussein, additional, Denis, Olivier, additional, Deplano, Ariane, additional, Vergison, Anne, additional, Rodriguez-Villalobos, Hector, additional, Tunney, Michael M., additional, Elborn, J. Stuart, additional, Kahl, Barbara C., additional, Traore, Hamidou, additional, Vanderbist, Francis, additional, Tulkens, Paul M., additional, and Van Bambeke, Françoise, additional
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- 2016
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25. Antimicrobial Susceptibility of Pseudomonas aeruginosaIsolated from Cystic Fibrosis Patients in Northern Europe
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Mustafa, Muhammad-Hariri, Chalhoub, Hussein, Denis, Olivier, Deplano, Ariane, Vergison, Anne, Rodriguez-Villalobos, Hector, Tunney, Michael M., Elborn, J. Stuart, Kahl, Barbara C., Traore, Hamidou, Vanderbist, Francis, Tulkens, Paul M., and Van Bambeke, Françoise
- Abstract
ABSTRACTPseudomonas aeruginosais a major cause of morbidity and mortality in cystic fibrosis patients. This study compared the antimicrobial susceptibilities of 153 P. aeruginosaisolates from the United Kingdom (UK) (n =58), Belgium (n =44), and Germany (n =51) collected from 118 patients during routine visits over the period from 2006 to 2012. MICs were measured by broth microdilution. Genes encoding extended-spectrum β-lactamases (ESBL), metallo-β-lactamases, and carbapenemases were detected by PCR. Pulsed-field gel electrophoresis and multilocus sequence typing were performed on isolates resistant to ≥3 antibiotic classes among the penicillins/cephalosporins, carbapenems, fluoroquinolones, aminoglycosides, and polymyxins. Based on EUCAST/CLSI breakpoints, susceptibility rates were ≤30%/≤40% (penicillins, ceftazidime, amikacin, and ciprofloxacin), 44 to 48%/48 to 63% (carbapenems), 72%/72% (tobramycin), and 92%/78% (colistin) independent of patient age. Sixty percent of strains were multidrug resistant (MDR; European Centre for Disease Prevention and Control criteria). Genes encoding the most prevalent ESBL (BEL, PER, GES, VEB, CTX-M, TEM, SHV, and OXA), metallo-β-lactamases (VIM, IMP, and NDM), or carbapenemases (OXA-48 and KPC) were not detected. The Liverpool epidemic strain (LES) was prevalent in UK isolates only (75% of MDR isolates). Four MDR sequence type 958 (ST958) isolates were found to be spread over the three countries. The other MDR clones were evidenced in ≤3 isolates and localized in a single country. A new sequence type (ST2254) was discovered in one MDR isolate in Germany. Clonal and nonclonal isolates with different susceptibility profiles were found in 20 patients. Thus, resistance and MDR are highly prevalent in routine isolates from 3 countries, with meropenem, tobramycin, and colistin remaining the most active drugs.
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- 2016
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26. Acquired resistance to macrolides in Pseudomonas aeruginosa from cystic fibrosis patients.
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Mustafa MH, Khandekar S, Tunney MM, Elborn JS, Kahl BC, Denis O, Plésiat P, Traore H, Tulkens PM, Vanderbist F, and Van Bambeke F
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- Administration, Oral, Adolescent, Adult, Cell Membrane metabolism, Child, Child, Preschool, Chronic Disease, Cystic Fibrosis drug therapy, Europe, Humans, Infant, Ketolides therapeutic use, Microbial Sensitivity Tests, Middle Aged, Mutation, Permeability, Phenotype, Ribosomes metabolism, Sequence Analysis, DNA, Young Adult, Anti-Bacterial Agents therapeutic use, Cystic Fibrosis microbiology, Drug Resistance, Bacterial genetics, Macrolides therapeutic use, Pseudomonas Infections drug therapy, Pseudomonas aeruginosa genetics
- Abstract
Cystic fibrosis (CF) patients receive chronic treatment with macrolides for their antivirulence and anti-inflammatory properties. We, however, previously showed that Pseudomonas aeruginosa , considered as naturally resistant to macrolides, becomes susceptible when tested in a eukaryotic medium rather than a conventional broth.We therefore looked for specific macrolide resistance determinants in 333 CF isolates from four European CF centres in comparison with 48 isolates from patients suffering from hospital-acquired pneumonia (HAP).Minimum inhibitory concentrations (MICs) of macrolides and ketolides measured in eukaryotic medium (RPMI-1640) were higher towards CF than HAP isolates. Gene sequencing revealed mutations at three positions (2045, 2046 and 2598) in domain V of 23S rRNA of 43% of sequenced CF isolates, but none in HAP isolates. Enzymes degrading extracellular polymeric substances also reduced MICs, highlighting a role of the mucoid, biofilm-forming phenotype in resistance. An association between high MICs and chronic azithromycin administration was evidenced, which was statistically significant for patients infected by the Liverpool Epidemic Strain.Thus, ribosomal mutations are highly prevalent in CF isolates and may spread in epidemic clones, arguing for prudent use of oral macrolides in these patients. Measuring MICs in RPMI-1640 could be easily implemented in microbiology laboratories to phenotypically detect resistance., Competing Interests: Conflict of interest: Disclosures can be found alongside this article at erj.ersjournals.com, (Copyright ©ERS 2017.)
- Published
- 2017
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