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Your search keyword '"Taylor, Ryan G."' showing total 7 results
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7 results on '"Taylor, Ryan G."'

1. Opioid-associated amnestic syndrome observed with fentanyl patch use

Author

Taylor, Ryan G., Budhram, Adrian, Lee, Donald H., and Mirsattari, Seyed M.

Subjects
Fentanyl -- Dosage and administration, Multiple sclerosis -- Drug therapy, Electroencephalography -- Usage, Cerebrospinal fluid -- Research, Postoperative pain, Pregabalin, Flumazenil, Topical analgesics, Health
Abstract

A 63-year-old woman with a history of multiple sclerosis and chronic musculoskeletal pain was found unresponsive in bed by her husband at about 1100 h. A year prior, the patient [...]

Published
2019
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3. The authors respond to 'Fentanyl patch probably not related to amnesia in case'

Author

Taylor, Ryan G., Mirsattari, Seyed M., and Lee, Donald H.

Subjects
Fentanyl, Central nervous system depressants, Health
Abstract

We thank Dr. Romayne Gallagher for her letter to the editor (1) regarding our article published in CMAJ, which describes a case of bilateral hippocampal injury and amnesia after pharmaceutical [...]

Published
2019

7. Measurement of Cdk1/Cyclin B Kinase Activity by Specific Antibodies and Western Blotting.

Author

Lewis CW, Taylor RG, and Golsteyn RM

Subjects
CDC2 Protein Kinase antagonists & inhibitors, Cell Cycle Checkpoints drug effects, Electrophoresis, Polyacrylamide Gel, Humans, Mitosis drug effects, Protein Kinase Inhibitors pharmacology, Antibody Specificity, Blotting, Western methods, CDC2 Protein Kinase immunology, CDC2 Protein Kinase metabolism, Cyclin B immunology, Cyclin B metabolism, Enzyme Assays methods
Abstract

Quantitative measurement of enzyme activity is a valuable approach to study how cells function. We present a method to measure the activity of the enzyme Cdk1/cyclin B. This enzyme is required by all eukaryotic cells to enter mitosis. Therefore, a biochemical assay to measure Cdk1/cyclin B activity can be used to identify cell populations that are in mitosis or to detect inhibitors of Cdk1/Cyclin B in vitro. A key distinction of the method presented here, compared to others, is that it uses a recombinant protein, a specific antibody, and a western blot apparatus, which makes the technique available to cell and molecular biology laboratories who do not wish to use radioisotopes, which are commonly required for other protein kinase assays.

Published
2016
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