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1,551 results on '"Sub Cell Biology"'

1. Erratum: Acute cellular and vascular responses to photodynamic therapy using EGFR-targeted nanobody-photosensitizer conjugates studied with intravital optical imaging and magnetic resonance imaging: Erratum

Author

Sub Cell Biology, Afd Pharmaceutics, Cell Biology, Neurobiology and Biophysics, Pharmaceutics, de Bruijn, Henriette S, Mashayekhi, Vida, Schreurs, Tom J L, van Driel, Pieter B A A, Strijkers, Gustav J, van Diest, Paul J, Lowik, Clemens W G M, Seynhaeve, Ann L B, Ten Hagen, Timo L M, Prompers, Jeanine J, van Bergen En Henegouwen, Paul M P, Robinson, Dominic J, Oliveira, Sabrina, Sub Cell Biology, Afd Pharmaceutics, Cell Biology, Neurobiology and Biophysics, Pharmaceutics, de Bruijn, Henriette S, Mashayekhi, Vida, Schreurs, Tom J L, van Driel, Pieter B A A, Strijkers, Gustav J, van Diest, Paul J, Lowik, Clemens W G M, Seynhaeve, Ann L B, Ten Hagen, Timo L M, Prompers, Jeanine J, van Bergen En Henegouwen, Paul M P, Robinson, Dominic J, and Oliveira, Sabrina

Published
2024

3. Opportunities for risk-taking during play alters cognitive performance and prefrontal inhibitory signalling in rats of both sexes

Author

AISS Behaviour Neuroscience, Dep Biologie, Sub Cell Biology, AISS – Behaviour Neuroscience, Bijlsma, Ate, Birza, Evelien, Pimentel, Tara, Maranus, Janneke, van Gaans, Marieke, Lozeman - van t Klooster, José, Baars, Anne-Marie, Achterberg, Marijke, Lesscher, Heidi, Wierenga, Corette, Vanderschuren, Louk, AISS Behaviour Neuroscience, Dep Biologie, Sub Cell Biology, AISS – Behaviour Neuroscience, Bijlsma, Ate, Birza, Evelien, Pimentel, Tara, Maranus, Janneke, van Gaans, Marieke, Lozeman - van t Klooster, José, Baars, Anne-Marie, Achterberg, Marijke, Lesscher, Heidi, Wierenga, Corette, and Vanderschuren, Louk

Published
2024

4. Cell scientist to watch - Ginny G. Farıás

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Farıás, Ginny Galdames, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, and Farıás, Ginny Galdames

Published
2024

7. The Role of MAPRE2 and Microtubules in Maintaining Normal Ventricular Conduction

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Chiang, DY, Verkerk, AO, Victorio, R, Shneyer, BI, van der Vaart, B, Jouni, M, Narendran, N, Kc, A, Sampognaro, JR, Vetrano-Olsen, F, Oh, JS, Buys, E, de Jonge, B, Shah, DA, Kiviniemi, T, Burridge, PW, Bezzina, CR, Akhmanova, A, Macrae, CA, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Chiang, DY, Verkerk, AO, Victorio, R, Shneyer, BI, van der Vaart, B, Jouni, M, Narendran, N, Kc, A, Sampognaro, JR, Vetrano-Olsen, F, Oh, JS, Buys, E, de Jonge, B, Shah, DA, Kiviniemi, T, Burridge, PW, Bezzina, CR, Akhmanova, A, and Macrae, CA

Published
2024

9. Photoimmuno-antimicrobial therapy for Staphylococcus aureus implant infection

Author

Afd Pharmaceutics, Sub Cell Biology, Pharmaceutics, Dijk, Bruce van, Oliveira, Sabrina, Hooning van Duyvenbode, J Fred F, Nurmohamed, F Ruben H A, Mashayekhi, Vida, Hernández, Irati Beltrán, van Strijp, Jos, de Vor, Lisanne, Aerts, Piet C, Vogely, H Charles, Weinans, Harrie, van der Wal, Bart C H, Afd Pharmaceutics, Sub Cell Biology, Pharmaceutics, Dijk, Bruce van, Oliveira, Sabrina, Hooning van Duyvenbode, J Fred F, Nurmohamed, F Ruben H A, Mashayekhi, Vida, Hernández, Irati Beltrán, van Strijp, Jos, de Vor, Lisanne, Aerts, Piet C, Vogely, H Charles, Weinans, Harrie, and van der Wal, Bart C H

Published
2024

10. Advances and Challenges in Cell Biology for Cultured Meat

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Martins, Beatriz, Bister, Arthur, Dohmen, Richard G.J., Gouveia, Maria Ana, Hueber, Rui, Melzener, Lea, Messmer, Tobias, Papadopoulos, Joanna, Pimenta, Joana, Raina, Dhruv, Schaeken, Lieke, Shirley, Sara, Bouchet, Benjamin P., Flack, Joshua E., Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Martins, Beatriz, Bister, Arthur, Dohmen, Richard G.J., Gouveia, Maria Ana, Hueber, Rui, Melzener, Lea, Messmer, Tobias, Papadopoulos, Joanna, Pimenta, Joana, Raina, Dhruv, Schaeken, Lieke, Shirley, Sara, Bouchet, Benjamin P., and Flack, Joshua E.

Published
2024

11. Nanobody-mediated targeting of zinc phthalocyanine with polymer micelles as nanocarriers

Author

Afd Pharmaceutics, Sub Membrane Biochemistry & Biophysics, Sub Cell Biology, Pharmaceutics, Mesquita, Bárbara, Singh, Arunika, Masdeu, Cèlia Prats, Lokhorst, Nienke, Hebels, Erik R, van Steenbergen, Mies, Mastrobattista, Enrico, Heger, Michal, van Nostrum, Cornelus F, Oliveira, Sabrina, Afd Pharmaceutics, Sub Membrane Biochemistry & Biophysics, Sub Cell Biology, Pharmaceutics, Mesquita, Bárbara, Singh, Arunika, Masdeu, Cèlia Prats, Lokhorst, Nienke, Hebels, Erik R, van Steenbergen, Mies, Mastrobattista, Enrico, Heger, Michal, van Nostrum, Cornelus F, and Oliveira, Sabrina

Published
2024

12. Social Play Behavior Is Critical for the Development of Prefrontal Inhibitory Synapses and Cognitive Flexibility in Rats

Author

Bijlsma, Ate, Omrani, Azar, Spoelder, Marcia, Verharen, Jeroen P H, Bauer, Lisa, Cornelis, Cosette, de Zwart, Beleke, van Dorland, René, Vanderschuren, Louk J M J, Wierenga, Corette J, AISS Behaviour Neuroscience, Sub Cell Biology, Celbiologie, AISS Behaviour Neuroscience, Sub Cell Biology, and Celbiologie

Subjects
Male, prefrontal cortex, social play, Neurogenesis, General Neuroscience, Vascular damage Radboud Institute for Health Sciences [Radboudumc 16], brain development, experience-dependent plasticity, Rats, Parvalbumins, Cognition, Synapses, Taverne, Animals, synaptic currents, cognitive performance, Research Articles
Abstract

Contains fulltext : 287797.pdf (Publisher’s version ) (Closed access) Sensory driven activity during early life is critical for setting up the proper connectivity of the sensory cortices. We ask here whether social play behavior, a particular form of social interaction that is highly abundant during postweaning development, is equally important for setting up connections in the developing prefrontal cortex (PFC). Young male rats were deprived from social play with peers during the period in life when social play behavior normally peaks [postnatal day 21-42] (SPD rats), followed by resocialization until adulthood. We recorded synaptic currents in layer 5 cells in slices from medial PFC of adult SPD and control rats and observed that inhibitory synaptic currents were reduced in SPD slices, while excitatory synaptic currents were unaffected. This was associated with a decrease in perisomatic inhibitory synapses from parvalbumin-positive GABAergic cells. In parallel experiments, adult SPD rats achieved more reversals in a probabilistic reversal learning (PRL) task, which depends on the integrity of the PFC, by using a more simplified cognitive strategy than controls. Interestingly, we observed that one daily hour of play during SPD partially rescued the behavioral performance in the PRL, but did not prevent the decrease in PFC inhibitory synaptic inputs. Our data demonstrate the importance of unrestricted social play for the development of inhibitory synapses in the PFC and cognitive skills in adulthood and show that specific synaptic alterations in the PFC can result in a complex behavioral outcome.SIGNIFICANCE STATEMENT This study addressed the question whether social play behavior in juvenile rats contributes to functional development of the prefrontal cortex (PFC). We found that rats that had been deprived from juvenile social play (social play deprivation - SPD) showed a reduction in inhibitory synapses in the PFC and a simplified strategy to solve a complex behavioral task in adulthood. Providing one daily hour of play during SPD partially rescued the cognitive skills in these rats, but did not prevent the reduction in PFC inhibitory synapses. Our results demonstrate a key role for unrestricted juvenile social play in PFC development and emphasize the complex relation between PFC circuit connectivity and cognitive function.

Published
2022

13. Teixobactin kills bacteria by a two-pronged attack on the cell envelope

Author

Shukla, Rhythm, Lavore, Francesca, Maity, Sourav, Derks, Maik G.N., Jones, Chelsea R., Vermeulen, Bram J.A., Melcrová, Adéla, Morris, Michael A., Becker, Lea Marie, Wang, Xiaoqi, Kumar, Raj, Medeiros-Silva, João, van Beekveld, Roy A.M., Bonvin, Alexandre M.J.J., Lorent, Joseph H., Lelli, Moreno, Nowick, James S., MacGillavry, Harold D., Peoples, Aaron J., Spoering, Amy L., Ling, Losee L., Hughes, Dallas E., Roos, Wouter H., Breukink, Eefjan, Lewis, Kim, Weingarth, Markus, Sub NMR Spectroscopy, Sub Membrane Biochemistry & Biophysics, Sub Cell Biology, NMR Spectroscopy, Celbiologie, Membrane Biochemistry and Biophysics, Sub NMR Spectroscopy, Sub Membrane Biochemistry & Biophysics, Sub Cell Biology, NMR Spectroscopy, Celbiologie, Membrane Biochemistry and Biophysics, and Molecular Biophysics

Subjects
Component, Target, Protein Structure, Secondary, Pyrrolidines, Nuclear Magnetic Resonance, Drug Resistance, Bacterial/drug effects, Drug Resistance, Anti-Bacterial Agents/chemistry, Bacteria/cytology, Lipids/chemistry, Microbial Sensitivity Tests, Molecular Dynamics Simulation, Microscopy, Atomic Force, Elucidation, Protein Structure, Secondary, Precursor lipid ii, Depsipeptides/chemistry, Cell Wall, Solid-state, Depsipeptides, Drug Resistance, Bacterial, Humans, Sugars/chemistry, Staphylococcus-aureus, General, Nuclear Magnetic Resonance, Biomolecular, Cell Wall/drug effects, Peptide antibiotics, Microscopy, Microbial Viability, Multidisciplinary, Bacteria, Cell Membrane/drug effects, Diphosphates/chemistry, Cell Membrane, Microbial Viability/drug effects, Bacterial/drug effects, Atomic Force, Enduracididine, Lipids, Nmr, Anti-Bacterial Agents, Diphosphates, Pyrrolidines/chemistry, Sugars, Analogs, Biomolecular
Abstract

Antibiotics that use novel mechanisms are needed to combat antimicrobial resistance1–3. Teixobactin4 represents a new class of antibiotics with a unique chemical scaffold and lack of detectable resistance. Teixobactin targets lipid II, a precursor of peptidoglycan5. Here we unravel the mechanism of teixobactin at the atomic level using a combination of solid-state NMR, microscopy, in vivo assays and molecular dynamics simulations. The unique enduracididine C-terminal headgroup of teixobactin specifically binds to the pyrophosphate-sugar moiety of lipid II, whereas the N terminus coordinates the pyrophosphate of another lipid II molecule. This configuration favours the formation of a β-sheet of teixobactins bound to the target, creating a supramolecular fibrillar structure. Specific binding to the conserved pyrophosphate-sugar moiety accounts for the lack of resistance to teixobactin4. The supramolecular structure compromises membrane integrity. Atomic force microscopy and molecular dynamics simulations show that the supramolecular structure displaces phospholipids, thinning the membrane. The long hydrophobic tails of lipid II concentrated within the supramolecular structure apparently contribute to membrane disruption. Teixobactin hijacks lipid II to help destroy the membrane. Known membrane-acting antibiotics also damage human cells, producing undesirable side effects. Teixobactin damages only membranes that contain lipid II, which is absent in eukaryotes, elegantly resolving the toxicity problem. The two-pronged action against cell wall synthesis and cytoplasmic membrane produces a highly effective compound targeting the bacterial cell envelope. Structural knowledge of the mechanism of teixobactin will enable the rational design of improved drug candidates.

Published
2022

14. Estimation of microtubule-generated forces using a DNA origami nanospring

Author

Nick Maleki, Ali, Huis In 't Veld, Pim J., Akhmanova, Anna, Dogterom, Marileen, Volkov, Vladimir A., Sub Cell Biology, Celbiologie, Sub Cell Biology, and Celbiologie

Subjects
Force sensor, Kinetochore, Microscopy, Fluorescence, Dynein, Microtubule, DNA origami, Cell Biology, Microtubules, Cytoskeleton, Mechanical Phenomena, Optical trap
Abstract

Microtubules are dynamic cytoskeletal filaments that can generate forces when polymerizing and depolymerizing. Proteins that follow growing or shortening microtubule ends and couple forces to cargo movement are important for a wide range of cellular processes. Quantifying these forces and the composition of protein complexes at dynamic microtubule ends is challenging and requires sophisticated instrumentation. Here, we present an experimental approach to estimate microtubule-generated forces through the extension of a fluorescent spring-shaped DNA origami molecule. Optical readout of the spring extension enables recording of force production simultaneously with single-molecule fluorescence of proteins getting recruited to the site of force generation. DNA nanosprings enable multiplexing of force measurements and only require a fluorescence microscope and basic laboratory equipment. We validate the performance of DNA nanosprings against results obtained using optical trapping. Finally, we demonstrate the use of the nanospring to study proteins that couple microtubule growth and shortening to force generation.

Published
2023

15. Incomplete abscission and cytoplasmic bridges in the evolution of eukaryotic multicellularity

Author

Chaigne, Agathe, Brunet, Thibaut, Celbiologie, Sub Cell Biology, Celbiologie, and Sub Cell Biology

Subjects
Cytoplasm, Cytosol, Eukaryotic Cells, Animals, Eukaryota, General Agricultural and Biological Sciences, Biological Evolution, General Biochemistry, Genetics and Molecular Biology
Abstract

The textbook view of cell division terminates with the final separation of the two daughter cells in the process called abscission. However, in contrast to this classical view, a variety of cell types in multicellular organisms are connected through cytoplasmic bridges, which most often form by incomplete abscission or — more rarely — by local fusion of plasma membranes. In this review, we survey the distribution, function, and formation of cytoplasmic bridges across the eukaryotic tree of life. We find that cytoplasmic bridges are widespread, and were likely ancestrally present, in almost all lineages of eukaryotes with clonal multicellularity — including the five ‘complex multicellular’ lineages: animals, fungi, land plants, red algae, and brown algae. In animals, cytoplasmic bridges resulting from incomplete abscission are ubiquitous in the germline and common in pluripotent cell types. Although cytoplasmic bridges have been less studied than other structural mediators of multicellularity (such as adhesion proteins and extracellular matrix), we propose that they have played a pivotal role in the repeated evolution of eukaryotic clonal multicellularity — possibly by first performing a structural role and later by allowing exchange of nutrients and/or intercellular communication, which notably buffered cell–cell competition by averaging gene expression. Bridges were eventually lost from many animal tissues in concert with the evolution of spatial cell differentiation, cell motility within the organism, and other mechanisms for intercellular distribution of signals and metabolites. Finally, we discuss the molecular basis for the evolution of incomplete abscission and examine the alternative hypotheses of single or multiple origins.

Published
2022

16. Parvalbumin basket cell myelination accumulates axonal mitochondria to internodes

Author

Kole, Koen, Voesenek, Bas J B, Brinia, Maria E, Petersen, Naomi, Kole, Maarten H P, Sub Cell Biology, Celbiologie, Sub Cell Biology, Celbiologie, and Netherlands Institute for Neuroscience (NIN)

Subjects
Myelin Sheath/metabolism, Action Potentials/physiology, Demyelinating Diseases/metabolism, Multidisciplinary, Action Potentials, General Physics and Astronomy, General Chemistry, Axons/metabolism, Interneurons/physiology, Axons, General Biochemistry, Genetics and Molecular Biology, Parvalbumins, nervous system, Interneurons, Humans, Parvalbumins/metabolism, Myelin Sheath, Demyelinating Diseases
Abstract

Parvalbumin-expressing (PV+) basket cells are fast-spiking inhibitory interneurons that exert critical control over local circuit activity and oscillations. PV+ axons are often myelinated, but the electrical and metabolic roles of interneuron myelination remain poorly understood. Here, we developed viral constructs allowing cell type-specific investigation of mitochondria with genetically encoded fluorescent probes. Single-cell reconstructions revealed that mitochondria selectively cluster to myelinated segments of PV+ basket cells, confirmed by analyses of a high-resolution electron microscopy dataset. In contrast to the increased mitochondrial densities in excitatory axons cuprizone-induced demyelination abolished mitochondrial clustering in PV+ axons. Furthermore, with genetic deletion of myelin basic protein the mitochondrial clustering was still observed at internodes wrapped by noncompacted myelin, indicating that compaction is dispensable. Finally, two-photon imaging of action potential-evoked calcium (Ca2+) responses showed that interneuron myelination attenuates both the cytosolic and mitochondrial Ca2+ transients. These findings suggest that oligodendrocyte ensheathment of PV+ axons assembles mitochondria to branch selectively fine-tune metabolic demands.

Published
2022

17. Directing HIV-1 for degradation by non-target cells, using bi-specific single-chain llama antibodies

Author

Stam, Jord C., De maat, Steven, De jong, Dorien, Arens, Mathia, Van lint, Fenna, Gharu, Lavina, Van roosmalen, Mark H., Roovers, Rob C., Strokappe, Nika M., Wagner, Ralf, Kliche, Alexander, De haard, Hans J., Van bergen en henegouwen, Paul M., Nijhuis, Monique, Verrips, C. Theo, Celbiologie, Sub Cell Biology, Celbiologie, and Sub Cell Biology

Subjects
Multidisciplinary, HIV Infections, New World, HIV Antibodies, Antibodies, Neutralizing, Antibodies, ErbB Receptors, Camelids, HIV Seropositivity, HIV-1, Animals, Humans, General, Neutralizing, Camelids, New World, Single-Chain Antibodies
Abstract

While vaccination against HIV-1 has been so far unsuccessful, recently broadly neutralizing antibodies (bNAbs) against HIV-1 envelope glycoprotein were shown to induce long-term suppression in the absence of antiretroviral therapy in patients with antibody-sensitive viral reservoirs. The requirement of neutralizing antibodies indicates that the antibody mediated removal (clearance) of HIV-1 in itself is not efficient enough in these immune compromised patients. Here we present a novel, alternative approach that is independent of a functional immune system to clear HIV-1, by capturing the virus and redirecting it to non-target cells where it is internalized and degraded. We use bispecific antibodies with domains derived from small single chain Llama antibodies (VHHs). These bind with one domain to HIV-1 envelope proteins and with the other domain direct the virus to cells expressing epidermal growth factor receptor (EGFR), a receptor that is ubiquitously expressed in the body. We show that HIV envelope proteins, virus-like particles and HIV-1 viruses (representing HIV-1 subtypes A, B and C) are efficiently recruited to EGFR, internalized and degraded in the lysosomal pathway at low nM concentrations of bispecific VHHs. This directed degradation in non-target cells may provide a clearance platform for the removal of viruses and other unwanted agents from the circulation, including toxins, and may thus provide a novel method for curing.

Published
2022

18. Structural insights into the non-inhibitory mechanism of the anti-EGFR EgB4 nanobody

Author

Zeronian, Matthieu R., Doulkeridou, Sofia, Van bergen en henegouwen, Paul M. P., Janssen, Bert J. C., Sub Structural Biochemistry, Sub Cell Biology, Celbiologie, Structural Biochemistry, Sub Structural Biochemistry, Sub Cell Biology, Celbiologie, and Structural Biochemistry

Subjects
EGFR-EGF, Antibodies, Monoclonal, Structure, Cell Biology, Single-Domain Antibodies, Ligands, X-ray diffraction, Epitopes, Neoplasms, Nanobody, Humans, Molecular Biology, Receptor
Abstract

Background The epidermal growth factor receptor (EGFR) is involved in various developmental processes, and alterations of its extracellular segment are associated with several types of cancers, in particular glioblastoma multiforme (GBM). The EGFR extracellular region is therefore a primary target for therapeutic agents, such as monoclonal antibodies and variable domains of heavy chain antibodies (VHH), also called nanobodies. Nanobodies have been previously shown to bind to EGFR, and to inhibit ligand-mediated EGFR activation. Results Here we present the X-ray crystal structures of the EgB4 nanobody, alone (to 1.48 Å resolution) and bound to the full extracellular EGFR-EGF complex in its active conformation (to 6.0 Å resolution). We show that EgB4 binds to a new epitope located on EGFR domains I and II, and we describe the molecular mechanism by which EgB4 plays a non-inhibitory role in EGFR signaling. Conclusion This work provides the structural basis for the application of EgB4 as a tool for research, for targeted therapy, or as a biomarker to locate EGFR-associated tumors, all without affecting EGFR activation.

Published
2022

19. Opto-katanin, an optogenetic tool for localized, microtubule disassembly

Author

Meiring, Joyce C M, Grigoriev, Ilya, Nijenhuis, Wilco, Kapitein, Lukas C, Akhmanova, Anna, Sub Cell Biology, Celbiologie, Sub Cell Biology, and Celbiologie

Subjects
Adenosine Triphosphatases, Agricultural and Biological Sciences(all), katanin, Biochemistry, Genetics and Molecular Biology(all), Neuroscience(all), Mitosis, opto-katanin, tool, optogenetic, Biochemistry, General Biochemistry, Genetics and Molecular Biology, neuron, Optogenetics, microtubules, ER, severing, transport, Golgi, General Agricultural and Biological Sciences, Genetics and Molecular Biology(all)
Abstract

Microtubules are cytoskeletal polymers that separate chromosomes during mitosis and serve as rails for intracellular transport and organelle positioning. Manipulation of microtubules is widely used in cell and developmental biology, but tools for precise subcellular spatiotemporal control of microtubules are currently lacking. Here, we describe a light-activated system for localized recruitment of the microtubule-severing enzyme katanin. This system, named opto-katanin, uses targeted illumination with blue light to induce rapid, localized, and reversible microtubule depolymerization. This tool allows precise clearing of a subcellular region of microtubules while preserving the rest of the microtubule network, demonstrating that regulation of katanin recruitment to microtubules is sufficient to control its severing activity. The tool is not toxic in the absence of blue light and can be used to disassemble both dynamic and stable microtubules in primary neurons as well as in dividing cells. We show that opto-katanin can be used to locally block vesicle transport and to clarify the dependence of organelle morphology and dynamics on microtubules. Specifically, our data indicate that microtubules are not required for the maintenance of the Golgi stacks or the tubules of the endoplasmic reticulum but are needed for the formation of new membrane tubules. Finally, we demonstrate that this tool can be applied to study the contribution of microtubules to cell mechanics by showing that microtubule bundles can exert forces constricting the nucleus.

Published
2022

20. Local changes in microtubule network mobility instruct neuronal polarization and axon specification

Author

Burute, Mithila, Jansen, Klara I, Mihajlovic, Marko, Vermonden, Tina, Kapitein, Lukas C, Celbiologie, Pharmaceutics, Sub Cell Biology, Afd Pharmaceutics, Celbiologie, Pharmaceutics, Sub Cell Biology, and Afd Pharmaceutics

Subjects
Neurons, Cultured, Multidisciplinary, Cells, Hippocampus/metabolism, Kinesins, Cell Polarity, Cell Polarity/physiology, Axons/metabolism, Hippocampus, Microtubules, Axons, Microtubules/metabolism, Neurons/physiology, Cells, Cultured
Abstract

The polarization of neurons into axons and dendrites depends on extracellular cues, intracellular signaling, cytoskeletal rearrangements, and polarized transport, but the interplay between these processes during polarization remains unresolved. Here, we show that axon specification is determined by differences in microtubule network mobility between neurites, regulated by Rho guanosine triphosphatases (GTPases) and extracellular cues. In developing neurons, retrograde microtubule flow prevents the entry of the axon-selective motor protein Kinesin-1 into most neurites. Using inducible assays to control microtubule network flow, we demonstrate that local inhibition of microtubule mobility is sufficient to guide Kinesin-1 into a specific neurite, whereas long-term global inhibition induces the formation of multiple axons. We furthermore show that extracellular mechanical cues and intracellular Rho GTPase signaling control the local differences in microtubule network flow. These results reveal a novel cytoskeletal mechanism for neuronal polarization.

Published
2022

22. A kinesin-based approach for inducing chromosome-specific mis-segregation in human cells

Author

Sub Cell Biology, Celbiologie, Truong, My Anh, Cané-Gasull, Paula, de Vries, Sippe G, Nijenhuis, Wilco, Wardenaar, René, Kapitein, Lukas C, Foijer, Floris, Lens, Susanne, Sub Cell Biology, Celbiologie, Truong, My Anh, Cané-Gasull, Paula, de Vries, Sippe G, Nijenhuis, Wilco, Wardenaar, René, Kapitein, Lukas C, Foijer, Floris, and Lens, Susanne

Published
2023

23. Estimation of microtubule-generated forces using a DNA origami nanospring

Author

Sub Cell Biology, Celbiologie, Nick Maleki, Ali, Huis In 't Veld, Pim J., Akhmanova, Anna, Dogterom, Marileen, Volkov, Vladimir A., Sub Cell Biology, Celbiologie, Nick Maleki, Ali, Huis In 't Veld, Pim J., Akhmanova, Anna, Dogterom, Marileen, and Volkov, Vladimir A.

Published
2023

28. Delaying the GABA Shift Indirectly Affects Membrane Properties in the Developing Hippocampus

Author

Sub Cell Biology, Innovation and Sustainability, Cell Biology, Neurobiology and Biophysics, Copernicus Institute of Sustainable Development, Peerboom, Carlijn, de Kater, Sam, Jonker, Nikki, Rieter, Marijn P.J.M., Wijne, Tessel, Wierenga, Corette J., Sub Cell Biology, Innovation and Sustainability, Cell Biology, Neurobiology and Biophysics, Copernicus Institute of Sustainable Development, Peerboom, Carlijn, de Kater, Sam, Jonker, Nikki, Rieter, Marijn P.J.M., Wijne, Tessel, and Wierenga, Corette J.

Published
2023

32. Plasticity of postsynaptic nanostructure

Author

Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Droogers, W J, MacGillavry, H D, Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Droogers, W J, and MacGillavry, H D

Published
2023

34. Exosomal release of the virus-encoded chemokine receptor US28 contributes to chemokine scavenging

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Bebelman, Maarten P, Setiawan, Irfan M, Bergkamp, Nick D, van Senten, Jeffrey R, Crudden, Caitrin, Bebelman, Jan Paul M, Verweij, Frederik J, van Niel, Guillaume, Siderius, Marco, Pegtel, D Michiel, Smit, Martine J, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Bebelman, Maarten P, Setiawan, Irfan M, Bergkamp, Nick D, van Senten, Jeffrey R, Crudden, Caitrin, Bebelman, Jan Paul M, Verweij, Frederik J, van Niel, Guillaume, Siderius, Marco, Pegtel, D Michiel, and Smit, Martine J

Published
2023

36. Compressive forces stabilize microtubules in living cells

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Li, Yuhui, Kučera, Ondřej, Cuvelier, Damien, Rutkowski, David M., Deygas, Mathieu, Rai, Dipti, Pavlovič, Tonja, Vicente, Filipe Nunes, Piel, Matthieu, Giannone, Grégory, Vavylonis, Dimitrios, Akhmanova, Anna, Blanchoin, Laurent, Théry, Manuel, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Li, Yuhui, Kučera, Ondřej, Cuvelier, Damien, Rutkowski, David M., Deygas, Mathieu, Rai, Dipti, Pavlovič, Tonja, Vicente, Filipe Nunes, Piel, Matthieu, Giannone, Grégory, Vavylonis, Dimitrios, Akhmanova, Anna, Blanchoin, Laurent, and Théry, Manuel

Published
2023

37. Aquaporin-4 and GPRC5B: old and new players in controlling brain oedema

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Passchier, Emma M J, Kerst, Sven, Brouwers, Eelke, Hamilton, Eline M C, Bisseling, Quinty, Bugiani, Marianna, Waisfisz, Quinten, Kitchen, Philip, Unger, Lucas, Breur, Marjolein, Hoogterp, Leoni, de Vries, Sharon I, Abbink, Truus E M, Kole, Maarten H P, Leurs, Rob, Vischer, Henry F, Brignone, Maria S, Ambrosini, Elena, Feillet, François, Born, Alfred P, Epstein, Leon G, Mansvelder, Huibert D, Min, Rogier, van der Knaap, Marjo S, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Passchier, Emma M J, Kerst, Sven, Brouwers, Eelke, Hamilton, Eline M C, Bisseling, Quinty, Bugiani, Marianna, Waisfisz, Quinten, Kitchen, Philip, Unger, Lucas, Breur, Marjolein, Hoogterp, Leoni, de Vries, Sharon I, Abbink, Truus E M, Kole, Maarten H P, Leurs, Rob, Vischer, Henry F, Brignone, Maria S, Ambrosini, Elena, Feillet, François, Born, Alfred P, Epstein, Leon G, Mansvelder, Huibert D, Min, Rogier, and van der Knaap, Marjo S

Published
2023

38. Taxanes trigger cancer cell killing in vivo by inducing non-canonical T cell cytotoxicity

Author

Sub Cell Biology, Sub Biomol.Mass Spectrometry & Proteom., Cell Biology, Neurobiology and Biophysics, Biomolecular Mass Spectrometry and Proteomics, Vennin, Claire, Cattaneo, Chiara M, Bosch, Leontien, Vegna, Serena, Ma, Xuhui, Damstra, Hugo G J, Martinovic, Moreno, Tsouri, Efi, Ilic, Mila, Azarang, Leyla, van Weering, Jan R T, Pulver, Emilia, Zeeman, Amber L, Schelfhorst, Tim, Lohuis, Jeroen O, Rios, Anne C, Dekkers, Johanna F, Akkari, Leila, Menezes, Renee, Medema, Rene, Baglio, Serena R, Akhmanova, Anna, Linn, Sabine C, Lemeer, Simone, Pegtel, Dirk M, Voest, Emile E, van Rheenen, Jacco, Sub Cell Biology, Sub Biomol.Mass Spectrometry & Proteom., Cell Biology, Neurobiology and Biophysics, Biomolecular Mass Spectrometry and Proteomics, Vennin, Claire, Cattaneo, Chiara M, Bosch, Leontien, Vegna, Serena, Ma, Xuhui, Damstra, Hugo G J, Martinovic, Moreno, Tsouri, Efi, Ilic, Mila, Azarang, Leyla, van Weering, Jan R T, Pulver, Emilia, Zeeman, Amber L, Schelfhorst, Tim, Lohuis, Jeroen O, Rios, Anne C, Dekkers, Johanna F, Akkari, Leila, Menezes, Renee, Medema, Rene, Baglio, Serena R, Akhmanova, Anna, Linn, Sabine C, Lemeer, Simone, Pegtel, Dirk M, Voest, Emile E, and van Rheenen, Jacco

Published
2023

39. CSPP1 stabilizes growing microtubule ends and damaged lattices from the luminal side

Author

Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, van den Berg, Cyntha M., Volkov, Vladimir A., Schnorrenberg, Sebastian, Huang, Ziqiang, Stecker, Kelly E., Grigoriev, Ilya, Gilani, Sania, Frikstad, Kari Anne M., Patzke, Sebastian, Zimmermann, Timo, Dogterom, Marileen, Akhmanova, Anna, Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, van den Berg, Cyntha M., Volkov, Vladimir A., Schnorrenberg, Sebastian, Huang, Ziqiang, Stecker, Kelly E., Grigoriev, Ilya, Gilani, Sania, Frikstad, Kari Anne M., Patzke, Sebastian, Zimmermann, Timo, Dogterom, Marileen, and Akhmanova, Anna

Published
2023

40. Sodium channel endocytosis drives axon initial segment plasticity

Author

Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, Kole, Maarten H P, Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, and Kole, Maarten H P

Published
2023

41. GelMap: intrinsic calibration and deformation mapping for expansion microscopy

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Damstra, Hugo G.J., Passmore, Josiah B., Serweta, Albert K., Koutlas, Ioannis, Burute, Mithila, Meye, Frank J., Akhmanova, Anna, Kapitein, Lukas C., Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Damstra, Hugo G.J., Passmore, Josiah B., Serweta, Albert K., Koutlas, Ioannis, Burute, Mithila, Meye, Frank J., Akhmanova, Anna, and Kapitein, Lukas C.

Published
2023

42. Human voltage-gated Na+ and K+ channel properties underlie sustained fast AP signaling

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Wilbers, René, Metodieva, Verjinia D, Duverdin, Sarah, Heyer, Djai B, Galakhova, Anna A, Mertens, Eline J, Versluis, Tamara D, Baayen, Johannes C, Idema, Sander, Noske, David P, Verburg, Niels, Willemse, Ronald B, de Witt Hamer, Philip C, Kole, Maarten H P, de Kock, Christiaan P J, Mansvelder, Huibert D, Goriounova, Natalia A, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Wilbers, René, Metodieva, Verjinia D, Duverdin, Sarah, Heyer, Djai B, Galakhova, Anna A, Mertens, Eline J, Versluis, Tamara D, Baayen, Johannes C, Idema, Sander, Noske, David P, Verburg, Niels, Willemse, Ronald B, de Witt Hamer, Philip C, Kole, Maarten H P, de Kock, Christiaan P J, Mansvelder, Huibert D, and Goriounova, Natalia A

Published
2023

45. Ten-fold Robust Expansion Microscopy

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Damstra, Hugo G.J., Mohar, Boaz, Eddison, Mark, Akhmanova, Anna, Kapitein, Lukas C., Tillberg, Paul W., Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Damstra, Hugo G.J., Mohar, Boaz, Eddison, Mark, Akhmanova, Anna, Kapitein, Lukas C., and Tillberg, Paul W.

Published
2023

47. Penetration of Nanobody-Dextran Polymer Conjugates through Tumor Spheroids

Author

Sub Cell Biology, Afd Pharmaceutics, Cell Biology, Neurobiology and Biophysics, Pharmaceutics, Bitsch, Peter, Baum, Eva S., Beltrán Hernández, Irati, Bitsch, Sebastian, Harwood, Jakob, Oliveira, Sabrina, Kolmar, Harald, Sub Cell Biology, Afd Pharmaceutics, Cell Biology, Neurobiology and Biophysics, Pharmaceutics, Bitsch, Peter, Baum, Eva S., Beltrán Hernández, Irati, Bitsch, Sebastian, Harwood, Jakob, Oliveira, Sabrina, and Kolmar, Harald

Published
2023

48. Active mesh and neural network pipeline for cell aggregate segmentation

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Smith, Matthew B., Sparks, Hugh, Almagro, Jorge, Chaigne, Agathe, Behrens, Axel, Dunsby, Chris, Salbreux, Guillaume, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Smith, Matthew B., Sparks, Hugh, Almagro, Jorge, Chaigne, Agathe, Behrens, Axel, Dunsby, Chris, and Salbreux, Guillaume

Published
2023

49. Ultrastructural Axon–Myelin Unit Alterations in Multiple Sclerosis Correlate with Inflammation

Author

Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Bosch, Aletta M. R. van den, Hümmert, Sophie, Steyer, Anna, Ruhwedel, Torben, Hamann, Jörg, Smolders, Joost, Nave, Klaus‐Armin, Stadelmann, Christine, Kole, Maarten H. P., Möbius, Wiebke, Huitinga, Inge, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Bosch, Aletta M. R. van den, Hümmert, Sophie, Steyer, Anna, Ruhwedel, Torben, Hamann, Jörg, Smolders, Joost, Nave, Klaus‐Armin, Stadelmann, Christine, Kole, Maarten H. P., Möbius, Wiebke, and Huitinga, Inge

Published
2023

50. What risk factors for Developmental Language Disorder can tell us about the neurobiological mechanisms of language development

Author

ILS LAPD, LS Psycholinguistiek, Experimental Psychology (onderzoeksprogramma PF), Helmholtz Institute, LS English Linguistics:Language Developm, Leerstoel Blom, Education and Learning: Cognitive and Motor Disabilities, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Boerma, Tessel, ter Haar, Sita, Ganga, Rachida, Wijnen, Frank, Blom, Elma, Wierenga, Corette, ILS LAPD, LS Psycholinguistiek, Experimental Psychology (onderzoeksprogramma PF), Helmholtz Institute, LS English Linguistics:Language Developm, Leerstoel Blom, Education and Learning: Cognitive and Motor Disabilities, Sub Cell Biology, Cell Biology, Neurobiology and Biophysics, Boerma, Tessel, ter Haar, Sita, Ganga, Rachida, Wijnen, Frank, Blom, Elma, and Wierenga, Corette

Published
2023

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