1. Determination of ibuprofen and paraben in pharmaceutical formulations using flow-injection and derivative spectrophotometry
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Turk, Sevilay Camalan, Satana, Eda, Basa, Hasan, and Goger, Nilgun Gunden
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Parabens -- Analysis ,Ibuprofen -- Analysis ,High performance liquid chromatography -- Analysis -- Methods ,Drugs -- Analysis ,Spectrum analysis -- Methods -- Analysis ,Chemistry - Abstract
A flow-injection UV spectrophotometric method for the determination of ibuprofen in tablets was developed. The proposed method was achieved by using a flow cell which was located in a UV spectrophotometer and a peristaltic pump helped maintaining the carrier solvent, 0.1 M NaOH. During the study, flow rate, loop volume and the number of injections per hour were 15 mL/min, 193 µL and 100, respectively. The analytical signal of ibuprofen was linear in the concentration range of 400-1200 µg/mL. The detection limit and limit of quantification were determined as 27 and 91 µg/mL, respectively. Results for the determination of ibuprofen in tablets, 600 ± 2 mg/tablet (mean ± SD), were in good agreement with the labeled quantities, 600 mg/tablet. A relatively high recovery value, 100.4 ± 0.6%, proves the accuracy of the proposed method. The spectrophotometric method and reference HPLC method were shown to be comparable when Student's f-test and Fisher test were applied to both methods. In addition, a fourth order derivative UV spectrophotometric method (at 268.8 nm and n = 9) for the determination of ibuprofen and a first order (at 271 nm and n = 1) derivative UV spectrophotometric method for the determination of paraben in pediatric syrups were developed. Recovery values for the determination of ibuprofen and paraben were 102.4 ± 1.1% and 100.2 ± 2.3%, respectively. Keywords: ibuprofen, flow-injection analysis, UV--VIS derivative spectroscopy, HPLC, paraben, pediatric syrups DOI: 10.1134/S1061934815010141, Ibuprofen, a-methyl-4-(2-methylpropyl)benzeneacetic acid, is a derivative of phenylpropionic acid and is used as a nonsteroidal anti-inflammatory agent. Several methods have been described for ibuprofen determination in pharmaceutical formulations including chromatography [...]
- Published
- 2015
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