Search

Your search keyword '"Sasaki, Tsutomu"' showing total 393 results
Start Over Author "Sasaki, Tsutomu" Publication Year Range Last 10 years
393 results on '"Sasaki, Tsutomu"'

4. Adaptive gene expression of alternative splicing variants of PGC-1α regulates whole-body energy metabolism

Author

Nomura, Kazuhiro, Kinoshita, Shinichi, Mizusaki, Nao, Senga, Yoko, Sasaki, Tsutomu, Kitamura, Tadahiro, Sakaue, Hiroshi, Emi, Aki, Hosooka, Tetsuya, Matsuo, Masahiro, Okamura, Hitoshi, Amo, Taku, Wolf, Alexander M., Kamimura, Naomi, Ohta, Shigeo, Itoh, Tomoo, Hayashi, Yoshitake, Kiyonari, Hiroshi, Krook, Anna, Zierath, Juleen R., Kasuga, Masato, and Ogawa, Wataru

Published
2024
Full Text
View/download PDF

6. Higher Frequency of Premature Atrial Contractions Correlates With Atrial Fibrillation Detection after Cryptogenic Stroke

Author

Shimada, Yuki, Todo, Kenichi, Doijiri, Ryosuke, Yamazaki, Hidekazu, Sonoda, Kazutaka, Koge, Junpei, Iwata, Tomonori, Ueno, Yuji, Yamagami, Hiroshi, Kimura, Naoto, Morimoto, Masafumi, Kondo, Daisuke, Koga, Masatoshi, Nagata, Eiichiro, Miyamoto, Nobukazu, Kimura, Yoko, Gon, Yasufumi, Okazaki, Shuhei, Sasaki, Tsutomu, Mochizuki, Hideki, Sugiyama, Shintaro, Ohara, Mariko, Ikegami, Takeshi, Wadayama, Tomoya, Murakami, Yasutaka, Sakurai, Rei, Moriyama, Takuya, Oi, Kiyotaka, Ikenouchi, Hajime, Yamaguchi, Eriko, Egashira, Shuhei, Takizawa, Shunya, Honma, Kazunari, Nakayama, Taira, Kadokura, Ayana, Hanano, Hideyuki, and Yamamoto, Shiro

Published
2024
Full Text
View/download PDF

13. CREB coactivator CRTC1 in melanocortin‐4 receptor‐expressing cells regulate dietary fat intake.

Author

Matsumura, Shigenobu, Fujisawa, Miyu, Fujiwara, Mizuki, Okayama, Houko, Marutani, Miona, Nousou, Eri, Sasaki, Tsutomu, and Harada, Naoki

Abstract

Cyclic adenosine monophosphate‐response element‐binding protein‐1‐regulated transcription coactivator‐1 (CRTC1), a cytoplasmic coactivator that translocates to the nucleus in response to cAMP, is associated with obesity. We previously reported that CRTC1 deficiency in melanocortin‐4 receptor (MC4R)‐expressing neurons, which regulate appetite and energy metabolism in the brain, causes hyperphagia and obesity under a high‐fat diet (HFD). HFD is preferred for mice, and the dietary fat in HFD is the main factor contributing to its palatability. These findings, along with our previous results, suggest that CRTC1 regulates the appetite for dietary fat. Therefore, in this study, we aimed to investigate the dietary fat intake behavior and energy metabolism of MC4R neuron‐specific CRTC1 knockout mice fed soybean oil or lard. CRTC1 deficiency increased the intake of soybean oil and significantly increased body weight gain. Furthermore, obesity induced by soybean oil intake was partially due to leptin resistance. No significant changes in soybean oil intake were observed between young CRTC1‐deficient and wild‐type mice; however, soybean oil intake increased with age. Moreover, lard intake did not significantly affect the body weight. Overall, our findings highlighted the crucial role of CRTC1 in the regulation of spontaneous dietary fat intake. Furthermore, the role of CRTC1 becomes increasingly significant with age. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

14. Modulation of Ca2+ oscillation following ischemia and nicotinic acetylcholine receptors in primary cortical neurons by high-throughput analysis.

Author

Sasaki, Tsutomu, Hisada, Sunao, Kanki, Hideaki, Nunomura, Kazuto, Lin, Bangzhong, Nishiyama, Kumiko, Kawano, Tomohito, Matsumura, Shigenobu, and Mochizuki, Hideki

Subjects
*NICOTINIC acetylcholine receptors, *FREQUENCIES of oscillating systems, *NEURON analysis, *CEREBRAL ischemia, *METHYL aspartate receptors, *NICOTINIC receptors
Abstract

Calcium oscillations in primary neuronal cultures and iPSCs have been employed to investigate arrhythmogenicity and epileptogenicity in drug development. Previous studies have demonstrated that Ca2+ influx via NMDA and nicotinic acetylcholine receptors (nAChRs) modulates Ca2+ oscillations. Nevertheless, there has been no comprehensive investigation into the impact of ischemia or nAChR-positive allosteric modulators (PAM) drugs on Ca2+ oscillations at a level that would facilitate high-throughput screening. We investigated the effects of ischemia and nAChR subtypes or nAChR PAM agonists on Ca2+ oscillations in high-density 2D and 3D-sphere primary neuronal cultures using 384-well plates with FDSS-7000. Ischemia for 1 and 2 h resulted in an increase in the frequency of Ca2+ oscillations and a decrease in their amplitude in a time-dependent manner. The NMDA and AMPA receptor inhibition significantly suppressed Ca2+ oscillation. Inhibition of NR2A or NR2B had the opposite effect on Ca oscillations. The potentiation of ischemia-induced Ca2+ oscillations was significantly inhibited by the NMDA receptor antagonist, MK-801, and the frequency of these oscillations was suppressed by the NR2B inhibitor, Ro-256981. In the 3D-neurosphere, the application of an α7nAChR agonist increased the frequency of Ca2+ oscillations, whereas the activation of α4β2 had no effect. The combination of nicotine and PNU-120596 (type II PAM) affected the frequency and amplitude of Ca2+ oscillations in a manner distinct from that of type I PAM. These systems may be useful not only for detecting epileptogenicity but also in the search for neuroprotective agents against cerebral ischemia. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

17. Transcriptome Analysis Identified SPP1+ Monocytes as a Key in Extracellular Matrix Formation in Thrombi

Author

Kitano, Takaya, primary, Sasaki, Tsutomu, additional, Matsui, Takahiro, additional, Kohara, Masaharu, additional, Ogawa, Kotaro, additional, Todo, Kenichi, additional, Nakamura, Hajime, additional, Sugiura, Yuri, additional, Shimada, Yuki, additional, Okazaki, Shuhei, additional, Iida, Junichi, additional, Shimazu, Kohki, additional, Morii, Eiichi, additional, Sakaguchi, Manabu, additional, Nishio, Masami, additional, Yokoe, Masaru, additional, Kishima, Haruhiko, additional, and Mochizuki, Hideki, additional

Published
2024
Full Text
View/download PDF

20. The PDK1-FoxO1 signaling in adipocytes controls systemic insulin sensitivity through the 5-lipoxygenase–leukotriene B₄ axis

Author

Hosooka, Tetsuya, Hosokawa, Yusei, Matsugi, Kaku, Shinohara, Masakazu, Senga, Yoko, Tamori, Yoshikazu, Aoki, Chikako, Matsui, Sho, Sasaki, Tsutomu, Kitamura, Tadahiro, Kuroda, Masashi, Sakaue, Hiroshi, Nomura, Kazuhiro, Yoshino, Kei, Nabatame, Yuko, Itoh, Yoshito, Yamaguchi, Kanji, Hayashi, Yoshitake, Nakae, Jun, Accili, Domenico, Yokomizo, Takehiko, Seino, Susumu, Kasuga, Masato, and Ogawa, Wataru

Published
2020

21. Neurosecretory protein GL stimulates food intake, de novo lipogenesis, and onset of obesity.

Author

Iwakoshi-Ukena, Eiko, Shikano, Kenshiro, Kondo, Kunihiro, Taniuchi, Shusuke, Furumitsu, Megumi, Ochi, Yuta, Sasaki, Tsutomu, Okamoto, Shiki, Bentley, George E, Kriegsfeld, Lance J, Minokoshi, Yasuhiko, and Ukena, Kazuyoshi

Subjects
Hypothalamus, Animals, Rats, Obesity, Insulin, Nerve Tissue Proteins, Gene Expression Profiling, Eating, Lipogenesis, fat accumulation, food intake, hypothalamus, insulin, lipogenesis, neuropeptide, neuroscience, rat, Biochemistry and Cell Biology
Abstract

Mechanisms underlying the central regulation of food intake and fat accumulation are not fully understood. We found that neurosecretory protein GL (NPGL), a newly-identified neuropeptide, increased food intake and white adipose tissue (WAT) in rats. NPGL-precursor gene overexpression in the hypothalamus caused increases in food intake, WAT, body mass, and circulating insulin when fed a high calorie diet. Intracerebroventricular administration of NPGL induced de novo lipogenesis in WAT, increased insulin, and it selectively induced carbohydrate intake. Neutralizing antibody administration decreased the size of lipid droplets in WAT. Npgl mRNA expression was upregulated by fasting and low insulin levels. Additionally, NPGL-producing cells were responsive to insulin. These results point to NPGL as a novel neuronal regulator that drives food intake and fat deposition through de novo lipogenesis and acts to maintain steady-state fat level in concert with insulin. Dysregulation of NPGL may be a root cause of obesity.

Published
2017

25. Mechanical thrombectomy in acute ischemic stroke patients with left ventricular assist device

Author

Kitano, Takaya, Sakaguchi, Manabu, Yamagami, Hiroshi, Ishikawa, Tatsuya, Ishibashi-Ueda, Hatsue, Tanaka, Kanta, Okazaki, Shuhei, Sasaki, Tsutomu, Kadono, Yoshinori, Takagaki, Masatoshi, Nishida, Takeo, Nakamura, Hajime, Yanase, Masanobu, Fukushima, Norihide, Shiozawa, Masayuki, Toyoda, Kazunori, Takahashi, Jun C., Funatsu, Takayuki, Ryu, Bikei, Yoshioka, Daisuke, Toda, Koichi, Murayama, Shigeo, Kawamata, Takakazu, Kishima, Haruhiko, Sawa, Yoshiki, Mochizuki, Hideki, and Todo, Kenichi

Published
2020
Full Text
View/download PDF

31. microRNA-33 maintains adaptive thermogenesis via enhanced sympathetic nerve activity

Author

Horie, Takahiro, Nakao, Tetsushi, Miyasaka, Yui, Nishino, Tomohiro, Matsumura, Shigenobu, Nakazeki, Fumiko, Ide, Yuya, Kimura, Masahiro, Tsuji, Shuhei, Rodriguez, Randolph Ruiz, Watanabe, Toshimitsu, Yamasaki, Tomohiro, Xu, Sijia, Otani, Chiharu, Miyagawa, Sawa, Matsushita, Kazuki, Sowa, Naoya, Omori, Aoi, Tanaka, Jin, Nishimura, Chika, Nishiga, Masataka, Kuwabara, Yasuhide, Baba, Osamu, Watanabe, Shin, Nishi, Hitoo, Nakashima, Yasuhiro, Picciotto, Marina R., Inoue, Haruhisa, Watanabe, Dai, Nakamura, Kazuhiro, Sasaki, Tsutomu, Kimura, Takeshi, and Ono, Koh

Published
2021
Full Text
View/download PDF

35. RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS

Author

Shimizu, Mikito, primary, Shiraishi, Naoyuki, additional, Tada, Satoru, additional, Sasaki, Tsutomu, additional, Beck, Goichi, additional, Nagano, Seiichi, additional, Kinoshita, Makoto, additional, Sumi, Hisae, additional, Sugimoto, Tomoyuki, additional, Ishida, Yoko, additional, Koda, Toru, additional, Ishikura, Teruyuki, additional, Sugiyama, Yasuko, additional, Kihara, Keigo, additional, Kanakura, Minami, additional, Nakajima, Tsuneo, additional, Takeda, Shuko, additional, Takahashi, Masanori P., additional, Yamashita, Toshihide, additional, Okuno, Tatsusada, additional, and Mochizuki, Hideki, additional

Published
2023
Full Text
View/download PDF

40. Carnosol suppresses interleukin-6 production in mouse lungs injured by ischemia-reperfusion operation and in RAW264.7 macrophages treated with lipopolysaccharide

Author

Momozane, Toru, Kawamura, Tomohiro, Itoh, Yumi, Sanosaka, Masato, Sasaki, Tsutomu, Kanzaki, Ryu, Ose, Naoko, Funaki, Soichiro, Shintani, Yasushi, Minami, Masato, Okumura, Meinoshin, and Takemori, Hiroshi

Subjects
Interleukins -- Health aspects, Lung -- Health aspects, Lipopolysaccharides -- Health aspects, Biological sciences
Abstract

Carnosol is a naturally occurring herbal compound, known for its antioxidative properties. We previously found that carnosol protected mouse lungs from ischemia-reperfusion injury in ex vivo cultures. To elucidate the molecular mechanisms underpinning carnosol-mediated lung protection, we analyzed modes of interleukin-6 (IL-6) gene expression, which is associated with lung ischemia-reperfusion injury. Microarray analysis of mouse lungs suggested that IL-6 mRNA levels were elevated in the mouse lungs subjected to clamp-reperfusion, which was associated with elevated levels of other inflammatory modulators, such as activating transcription factor 3 (ATF3). Carnosol pretreatment lowered the IL-6 protein levels in mouse lung homogenates prepared after the clamp-reperfusion. On the other hand, the ATF3 gene expression was negatively correlated with that of IL-6 in RAW264.7 cells. IL-6 mRNA levels and gene promoter activities were suppressed by carnosol in RAW264.7 cells, but rescued by ATF3 knockdown. When RAW264.7 cells were subjected to hypoxia-reoxygenation, carnosol treatment lowered oxygen consumption after reoxygenation, which was coupled with a correlation with a transient production of mitochondrial reactive oxygen species and following ATF3 gene expression. These results suggest that carnosol treatment could be a new strategy for protecting lungs from ischemia-reperfusion injury by modulating the ATF3-IL-6 axis.Key words: carnosol, IL-6, ATF3, lung ischemia-reperfusion injury.Le carnosol est un compose naturel d'origine vegetale connu pour ses proprietes antioxydantes. Les auteurs avaient precedemment trouve que le carnosol protegeait le poumon de la souris du dommage d'ischemie-reperfiision en culture ex vivo. Afin d'elucider les mecanismes moleculaires qui sous-tendent la protection du poumon par le carnosol, ils ont analyse l'expression genique de l'interleukine-6 (IL-6), associee au dommage d'ischemie-reperfusion pulmonaire. Une analyse sur microreseau de poumons de souris a revele que les niveaux de l'ARNm d'IL-6 etaient eleves dans les poumons des souris soumises a un clamp-reperfusion, qui etaient associes a des niveaux eleves d'autres modulateurs inflammatoires tels que le facteur ATF3 (pour << activating transcription factor 3 >>). Un pretraitement au carnosol diminuait les niveaux proteiques d'IL-6 dans des homogenats de poumons prepares apres le clamp-reperfusion. Par contre, l'expression genique d'ATF3 etait correlee negativement avec celle de l'IL-6 chez les cellules RAW264.7. Les niveaux d'ARNm d'IL-6 et l'activite du promoteur du gene etaient diminues par le carnosol dans les cellules RAW264.7, mais retablis par le knock-down d'ATF3. Lorsque les cellules RAW264.7 etaient soumises a une hypoxie-reoxygenation, le traitement au carnosol diminuait la consommation d'oxygene apres la reoxygenation, diminution couplee a une production transitoire de derives reactifs d'oxygene mitochondriaux a la suite de l'expression d'ATF3. Ces resultats suggerent que le traitement au carnosol pourrait constituer une nouvelle strategie de protection des poumons des dommages d'ischemie-reperfusion en modulant l'axe ATF3-IL-6. [Traduit par la Redaction]Mots-cles: carnosol, IL-6, ATF3, dommage pulmonaire d'ischemie-reperfusion., IntroductionInflammatory cytokines play important roles in the development of tissue injury after ischemia-reperfusion (Beckers et al. 2017). Proper control of inflammatory signals is considered an excellent prognosis in surgery for [...]

Published
2018
Full Text
View/download PDF

41. Predictors of survival in patients with ischemic stroke and active cancer: A prospective, multicenter, observational study

Author

Gon, Yasufumi, primary, Sakaguchi, Manabu, additional, Yamagami, Hiroshi, additional, Abe, Soichiro, additional, Hashimoto, Hiroyuki, additional, Ohara, Nobuyuki, additional, Takahashi, Daisuke, additional, Abe, Yuko, additional, Takahashi, Tsutomu, additional, Kitano, Takaya, additional, Okazaki, Shuhei, additional, Todo, Kenichi, additional, Sasaki, Tsutomu, additional, Hattori, Satoshi, additional, and Mochizuki, Hideki, additional

Published
2023
Full Text
View/download PDF

42. Predictors of Survival in Patients With Ischemic Stroke and Active Cancer: A Prospective, Multicenter, Observational Study

Author

1000070751311, Gon, Yasufumi, Sakaguchi, Manabu, Yamagami, Hiroshi, Abe, Soichiro, Hashimoto, Hiroyuki, Ohara, Nobuyuki, Takahashi, Daisuke, Abe, Yuko, Takahashi, Tsutomu, Kitano, Takaya, 1000060623072, Okazaki, Shuhei, 1000040403068, Todo, Kenichi, 1000020534879, 0000-0001-8726-978X, Sasaki, Tsutomu, 1000050425154, Hattori, Satoshi, 1000090230044, 0000-0002-0874-7542, Mochizuki, Hideki, 1000070751311, Gon, Yasufumi, Sakaguchi, Manabu, Yamagami, Hiroshi, Abe, Soichiro, Hashimoto, Hiroyuki, Ohara, Nobuyuki, Takahashi, Daisuke, Abe, Yuko, Takahashi, Tsutomu, Kitano, Takaya, 1000060623072, Okazaki, Shuhei, 1000040403068, Todo, Kenichi, 1000020534879, 0000-0001-8726-978X, Sasaki, Tsutomu, 1000050425154, Hattori, Satoshi, 1000090230044, 0000-0002-0874-7542, and Mochizuki, Hideki

Abstract

Gon Y., Sakaguchi M., Yamagami H., et al. Predictors of Survival in Patients With Ischemic Stroke and Active Cancer: A Prospective, Multicenter, Observational Study. Journal of the American Heart Association 12, e029618 (2023); https://doi.org/10.1161/JAHA.123.029618., BACKGROUND: Limited data exist on the prognostic factors for patients with ischemic stroke and active cancer. METHODS AND RESULTS: We conducted a prospective, multicenter, observational study in Japan, including patients with acute ischemic stroke and active cancer, to investigate the prognostic factors. We followed up the patients for 1 year after stroke onset. The patients were divided into 2 groups according to cryptogenic stroke and known causes (small-vessel occlusion, large-artery atherosclerosis, cardioembolism, and other determined cause), and survival was compared. The hazard ratios (HRs) and 95% CIs for mortality were calculated using Cox regression models. We identified 135 eligible patients (39% women; median age, 75 years). Of these patients, 51% had distant metastasis. A total of 65 (48%) and 70 (52%) patients had cryptogenic stroke and known causes, respectively. Patients with cryptogenic stroke had significantly shorter survival than those with known causes (HR [95% CI], 3.11 [1.82–5.32]). The multivariable Cox regression analysis revealed that distant metastasis, plasma D-dimer levels, venous thromboembolism (either deep venous thrombosis or pulmonary embolism) complications at stroke onset were independent predictors of mortality after adjusting for potential confounders. Cryptogenic stroke was associated with prognosis in univariable analysis but was not significant in multivariable analysis. The plasma D-dimer levels stratified the prognosis of patients with ischemic stroke and active cancer. CONCLUSIONS: The prognosis of patients with acute ischemic stroke and active cancer varied considerably depending on stroke mechanism, distant metastasis, and coagulation abnormalities. The present study confirmed that coagulation abnormalities were crucial in determining the prognosis of such patients.

Published
2023

Catalog

Books, media, physical & digital resources
See catalog results