Search

Your search keyword '"Rubessa, Marcello"' showing total 38 results
Start Over Author "Rubessa, Marcello" Publication Year Range Last 10 years
38 results on '"Rubessa, Marcello"'

2. 102 Estradiol and follicular growth are associated with estrous behavior monitored by an accelerometer in grazing beef cows

Author

Rocha, Cecilia c Constantino, primary, Montevecchio, Ana B, additional, Mazziotta, Mackenzie, additional, Bennett, Alexandra, additional, Waheed, Abdul, additional, Campbell, Meghan, additional, Rubessa, Marcello, additional, Krisher, Rebecca, additional, Moriel, Philipe, additional, Hansen, Peter J, additional, Chebel, Ricardo, additional, and Binelli, Mario, additional

Published
2024
Full Text
View/download PDF

12. Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

Author

Rocha, Rebeca Magalhães Pedrosa, primary, Rubessa, Marcello, additional, Lima, Laritza Ferreira de, additional, Silva, Ana Flávia Bezerra da, additional, Winters, Rebecca, additional, Polkoff, Kathryn, additional, Milner, Derek, additional, Campello, Cláudio Cabral, additional, Figueiredo, Jose Ricardo de, additional, and Wheeler, Matthew, additional

Published
2021
Full Text
View/download PDF

13. Repair of critical-size porcine craniofacial bone defects using a collagen–polycaprolactone composite biomaterial

Author

Dewey, Marley J, primary, Milner, Derek J, additional, Weisgerber, Daniel, additional, Flanagan, Colleen L, additional, Rubessa, Marcello, additional, Lotti, Sammi, additional, Polkoff, Kathryn M, additional, Crotts, Sarah, additional, Hollister, Scott J, additional, Wheeler, Matthew B, additional, and Harley, Brendan A C, additional

Published
2021
Full Text
View/download PDF

14. Repair of critical-size porcine craniofacial bone defects using a collagen-polycaprolactone composite biomaterial

Author

Dewey, Marley J., primary, Milner, Derek J., additional, Weisgerber, Daniel, additional, Flanagan, Colleen L., additional, Rubessa, Marcello, additional, Lotti, Sammi, additional, Polkoff, Kathryn M., additional, Crotts, Sarah, additional, Hollister, Scott J., additional, Wheeler, Matthew B., additional, and Harley, Brendan A.C., additional

Published
2021
Full Text
View/download PDF

15. Estradiol and follicular growth are associated with estrous behavior monitored by an accelerometer in grazing beef cows.

Author

Constantino Rocha, Cecilia c., Montevecchio, Ana B., Mazziotta, Mackenzie, Bennett, Alexandra, Waheed, Abdul, Campbell, Meghan, Rubessa, Marcello, Krisher, Rebecca, Moriel, Philipe, Hansen, Peter J., Chebel, Ricardo, and Binelli, Mario

Subjects
ESTRUS, COWS, EMBRYO transfer, PREGNANCY outcomes, COW-calf system, ESTRADIOL, ACCELEROMETERS
Abstract

In beef cattle, 40% of cows that display estrus do not remain pregnant after embryo transfer (ET). Estrus is a physiological behavior influenced by the concentration of sex-steroids. The use of accelerometer devices for estrus detection is a common practice in dairy operations. It remains unclear if this same artificial intelligence software can accurately measure reproductive behavior in beef females. We hypothesized that estrous variables detected by an accelerometer are associated with ovarian, hormonal, pregnancy outcome, and fertility-related variables measured in the cow. Fifty Bos indicus-influenced, cyclic, primiparous cows were fitted with an accelerometer collar (SCR Engineers, Israel) and a breeding indicator (Estrotectâ) to detect estrus after a synchronization protocol in five subsequent experimental replicates (n = 50*5 = 250). Only cows that showed estrus (d 0) remained in the study. The following variables were used from the accelerometer software: Activity Peak, Rumination Nadir, Estrus Duration, and Heat Index, calculated by proprietary algorithms. Dominant follicle (DF) diameter and plasma concentration of estradiol were measured on d -2 (day of progesterone device removal) and on d 0. On d -1 and d 4, endometrial thickness and luminal fluid accumulation were measured by ultrasonography. On d 7, two embryos were transferred/cow. Pregnancies were diagnosed on d 45 and terminated. After 30 d, a new replicate started. Cows included in at least two replicates that remained pregnant 100%, 99-50%, or less than 50% were classified as fertile, sub fertile, and infertile, respectively. Estrus display was recorded for 166 cows (66%). Estrus detection by accelerometer and Estrotect had excellent agreement rate (kappa coefficient 0.76). Cows detected in estrus only by Estrotect had 66% less pregnancy/ET than cows in estrus by both (9/21 vs. 97/145; P = 0.01). The DF diameter on d 0 was not associated with accelerometer variables. Cows with greater and moderate Activity Peak had a greater growth rate of the DF from d -2 to d 0 than lower Activity Peak cows (interaction day*Activity Peak; P = 0.06). Estradiol on d -1 was positively associated with the Activity Peak (P < 0.0001). Cows with lower Activity Peak had a thicker endometrium on both d -1 and d 4 (P=0.02), and greater uterine luminal fluid score on d -1 (P=0.03). Fertility status was not associated with any accelerometer variables. In conclusion, the Activity Peak is associated positively with estradiol concentration and DF growth, however no association was found with pregnancy outcome and fertility. Therefore, the main advantage of accelerometers in beef ranches is the ability to detect estrus at a similar accuracy as breeding indicators, using less labor. Future enhancements in hardware and software to allow prediction of pregnancy outcomes are expected to increase reproductive efficiency in cow-calf operations. [ABSTRACT FROM AUTHOR]

Published
2024

18. Emergency ventilator for COVID-19

Author

King, William P., primary, Amos, Jennifer, additional, Azer, Magdi, additional, Baker, Daniel, additional, Bashir, Rashid, additional, Best, Catherine, additional, Bethke, Eliot, additional, Boppart, Stephen A., additional, Bralts, Elisabeth, additional, Corey, Ryan M., additional, Dietkus, Rachael, additional, Durack, Gary, additional, Elbel, Stefan, additional, Elliott, Greg, additional, Fava, Jake, additional, Goldenfeld, Nigel, additional, Goldstein, Molly H., additional, Hayes, Courtney, additional, Herndon, Nicole, additional, Jamison, Shandra, additional, Johnson, Blake, additional, Johnson, Harley, additional, Johnson, Mark, additional, Kolaczynski, John, additional, Lee, Tonghun, additional, Maslov, Sergei, additional, McGregor, Davis J., additional, Milner, Derek, additional, Moller, Ralf, additional, Mosley, Jonathan, additional, Musser, Andy, additional, Newberger, Max, additional, Null, David, additional, O’Bryan, Lucas, additional, Oelze, Michael, additional, O’Leary, Jerry, additional, Pagano, Alex, additional, Philpott, Michael, additional, Pianfetti, Brian, additional, Pille, Alex, additional, Pizzuto, Luca, additional, Ricconi, Brian, additional, Rubessa, Marcello, additional, Rylowicz, Sam, additional, Shipley, Clifford, additional, Singer, Andrew C., additional, Stewart, Brian, additional, Switzky, Rachel, additional, Tawfick, Sameh, additional, Wheeler, Matthew, additional, White, Karen, additional, Widloski, Evan M., additional, Wood, Eric, additional, Wood, Charles, additional, and Wooldridge, Abigail R., additional

Published
2020
Full Text
View/download PDF

19. “Which Factors Affect Pregnancy Until Calving and Pregnancy Loss in Buffalo Recipients of in vitro Produced Embryos?”

Author

Saliba, Wilson Pardini, primary, Gimenes, Lindsay Unno, additional, Drumond, Roberti Martins, additional, Bayão, Henrique Xavier Salgado, additional, Di Palo, Rossella, additional, Gasparrini, Bianca, additional, Rubessa, Marcello, additional, Baruselli, Pietro Sampaio, additional, Sales, José Nélio Souza, additional, Bastianetto, Eduardo, additional, Leite, Rômulo Cerqueira, additional, and Alvim, Mucio Túlio Teixeira, additional

Published
2020
Full Text
View/download PDF

26. ASSESSMENT OF VITRIFICATION-INDUCED STRUCTURAL DAMAGES IN MATURED BOVINE OOCYTES BY RAMAN MICROSPECTROSCOPY

Author

CAROTENUTO, ROSA, TUSSELLINO, MARGHERITA, GASPARRINI, BIANCA, RUSCIANO, GIULIA, De Canditiis, Carolina, Rubessa,Marcello, Carotenuto, Rosa, Tussellino, Margherita, Gasparrini, Bianca, Rusciano, Giulia, Rubessa, Marcello, and De Canditiis, Carolina

Subjects
RAMAN MICROSPECTROSCOPY, VITRIFICATION
Abstract

Vitrification induces ultrastructural and structural damages in different oocyte structures such as membrane, zona pellucida (ZP) and cytoplasm.1 Recently Raman Microspectroscopy (RMS) has been used to assess the changes caused by vitrification/ warming of in vitro matured ovine oocytes.2 RMS is a noninvasive technique for studying the molecular composition of cells, based on the inelastic scattering of laser photons by vibrating molecules. Aim of the present study was to investigate the structural modifications of ZP and cytoplasm of vitrified/warmed in vitro matured bovine oocytes by RMS. Abattoir derived in vitro matured oocytes (n=171) were denuded and divided in three experimental groups: control untreated oocytes (CTR), oocytes only exposed to vitrification/warming solutions (CPA) and vitrified/warmed oocytes (VITRI). Oocytes were exposed/vitrified in 20% EG + 20% of DMSO and 0.5 M sucrose and warmed into decreasing concentrations of sucrose (1.25 M-0.3 M). RMS analysis was carried out on CTR matured oocytes (after 22h) and on CPA and VITRI oocytes at different incubation times (0,1,2,3 and 4h) after exposure/warming. The analysis of the large spectral date set, acquired by RMS were performed by Principal Component Analysis (PCA). Our experimental outcomes suggest that vitrification induce a transformation of the protein secondary structure from the α-helices to the b-sheet form in the ZP, while lipids tend to assume a more ordered configuration. Both effects induce a mechanical stiffening of ZP, which could explain the reduced fertility of vitrified oocytes with respect to the untreated ones. Intriguingly, these transformations present a certain degree of reversibility, which renders vitrified oocytes more similar to untreated cells after 2h of warming at room temperature.

Published
2016

27. A Mineralized Collagen-Polycaprolactone Composite Promotes Healing of a Porcine Mandibular Defect

Author

Weisgerber, Daniel W., primary, Milner, Derek J., additional, Lopez-Lake, Heather, additional, Rubessa, Marcello, additional, Lotti, Sammi, additional, Polkoff, Kathryn, additional, Hortensius, Rebecca A., additional, Flanagan, Colleen L., additional, Hollister, Scott J., additional, Wheeler, Matthew B., additional, and Harley, Brendan A.C., additional

Published
2018
Full Text
View/download PDF

35. Non-invasive analysis of bovine embryo metabolites during in vitro embryo culture using nuclear magnetic resonance.

Author

Rubessa, Marcello, Ambrosi, Andrea, Gonzalez-Pena, Dianelys, Polkoff, Kathryn M., Denmark, Scott E., and Wheeler, Matthew B.

Subjects
*METABOLITE analysis, *NUCLEAR magnetic resonance, *SMALL molecules
Abstract

The ability to identify embryos that have the highest developmental potential from a cohort would significantly increase the chances of achieving pregnancy. Metabolic analysis is a well-established analytical approach in biological systems. Starting from this idea, we chose to use high-resolution nuclear magnetic resonance (1H-NMR) spectroscopy. The aim of this study was to determine if it is possible to select viable embryos after 48 h of culture using metabolic activity as the parameter. We evaluated embryo metabolism after the first 48 h of culture and compared the activity of cleaved embryos that became blastocysts to cleaved embryos that did not develop to blastocysts, and in vitro fertilized (IVF) blastocysts and parthenogenetic-activated (PA) blastocysts. Our results show that citrate, pyruvate, myo-inositol and lysine have great impact on predicting embryo development. When we compared IVF and PA blastocysts, we found that acetate and phenylalanine concentrations are excellent parameters for evaluating blastocyst quality. Combining all these results, we were able to create a formula that predicts zygote development after 2 days of culture. In conclusion, we found that it is possible predict the future development of in vitro produced bovine embryos after only 2 days of culture using 1H-NMR. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

37. Raman-microscopy investigation of vitrification-induced structural damages in mature bovine oocytes

Author

Giulia Rusciano, Rosa Carotenuto, Carolina De Canditiis, Gianluigi Zito, Bianca Gasparrini, María Roca, Antonio Sasso, Marcello Rubessa, Rusciano, Giulia, De Canditiis, Carolina, Zito, Gianluigi, Rubessa, Marcello, Roca, Maria Serena, Carotenuto, Rosa, Sasso, Antonio, and Gasparrini, Bianca

Subjects
0301 basic medicine, Cytoplasm, Embryology, lcsh:Medicine, Reproductive technology, Spectrum Analysis, Raman, Biochemistry, Oogenesis, Protein Structure, Secondary, Cryopreservation, Oxidative Damage, 0302 clinical medicine, Animal Cells, Macromolecular Structure Analysis, Vitrification, Birth Rate, Zona pellucida, lcsh:Science, Principal Component Analysis, 030219 obstetrics & reproductive medicine, Multidisciplinary, Fertility Preservation, Lipids, Cell biology, medicine.anatomical_structure, OVA, bovine oocytes, vitrification, Raman-microscopy, Female, Structural Proteins, Cellular Types, Cellular Structures and Organelles, Research Article, Protein Structure, Cell Survival, Specimen Preservation, Biology, Research and Analysis Methods, Cryobiology, 03 medical and health sciences, Botany, medicine, Animals, Blastocyst, Molecular Biology, Zona Pellucida, lcsh:R, Biology and Life Sciences, Proteins, Cell Biology, Oocyte cryopreservation, Lipid Metabolism, Oocyte, Germ Cells, 030104 developmental biology, Specimen Preparation and Treatment, Oocytes, Blastocysts, Cattle, lcsh:Q, Reactive Oxygen Species, Developmental Biology
Abstract

Although oocyte cryopreservation has great potentials in the field of reproductive technologies, it still is an open challenge in the majority of domestic animals and little is known on the biochemical transformation induced by this process in the different cellular compartments. Raman micro-spectroscopy allows the non-invasive evaluation of the molecular composition of cells, based on the inelastic scattering of laser photons by vibrating molecules. The aim of this work was to assess the biochemical modifications of both the zona pellucida and cytoplasm of vitrified/warmed in vitro matured bovine oocytes at different post-warming times. By taking advantage of Principal Component Analysis, we were able to shed light on the biochemical transformation induced by the cryogenic treatment, also pointing out the specific role of cryoprotective agents (CPs). Our results suggest that vitrification induces a transformation of the protein secondary structure from the α-helices to the β-sheet form, while lipids tend to assume a more packed configuration in the zona pellucida. Both modifications result in a mechanical hardening of this cellular compartment, which could account for the reduced fertility rates of vitrified oocytes. Furthermore, biochemical modifications were observed at the cytoplasmic level in the protein secondary structure, with α-helices loss, suggesting cold protein denaturation. In addition, a decrease of lipid unsaturation was found in vitrified oocytes, suggesting oxidative damages. Interestingly, most modifications were not observed in oocytes exposed to CPs, suggesting that they do not severely affect the biochemical architecture of the oocyte. Nevertheless, in oocytes exposed to CPs decreased developmental competence and increased reactive oxygen species production were observed compared to the control. A more severe reduction of cleavage and blastocyst rates after in vitro fertilization was obtained from vitrified oocytes. Our experimental outcomes also suggest a certain degree of reversibility of the induced transformations, which renders vitrified oocytes more similar to untreated cells after 2 h warming.

Published
2017

38. In Vitro Embryo Production in Buffalo Species (Bubalus bubalis).

Author

Rubessa M, Boccia L, and Di Francesco S

Subjects
Animals, Blastocyst cytology, Buffaloes, Female, Blastocyst metabolism, Embryo Culture Techniques methods, Embryonic Development, Fertilization in Vitro methods
Abstract

In the last years there has been a growing attention in the world regarding buffalo (Bubalus bubalis) breeding. In tropical countries, climatic and environmental conditions make buffalo an irreplaceable milk producer. Since cattle cannot express their own productive potential in these conditions, buffaloes are best suited to satisfy animal protein demand, as they are easy to maintain, long-lived, and parasite resistant. Here we describe how to produce buffalo embryos from slaughterhouse oocytes, with protocols for media formulation, oocyte selection, semen capacitation, and in the end embryo evaluation.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results