Search

We report the 1st example of a true complementarity effect in memory development--a situation in which memory for the "same event" simultaneously becomes more and less accurate between early childhood and adulthood. We investigated this paradoxical effect because fuzzy-trace theory predicts that it can occur in paradigms that produce developmental reversals in false memory, which are circumstances in which adults are more likely than children to remember new events as old. The complementarity prediction is this: If subjects separately judge whether those same events are new but similar to old ones, adults will be more accurate than children, even though adults are less accurate when they judge whether the items are old. We report 4 experiments in which children (6- and 10-year-olds), adolescents (14-year-olds), and adults encoded the modal developmental reversal materials: Deese-Roediger-McDermott lists. Then, they responded to memory tests on which half the subjects judged whether test items were old and half judged whether the same items were new-similar. The paradoxical complementarity effect was detected in all experiments: The tendency to falsely remember "new-similar" items as being old increased with development, but so did the tendency to correctly remember them as being new-similar.

Abstract The hepatocytes within the liver present an immense capacity to adapt to changes in nutrient availability. Here, by using high resolution volume electron microscopy, we map how hepatic subcellular spatial organization is regulated during nutritional fluctuations and as a function of liver zonation. We identify that fasting leads to remodeling of endoplasmic reticulum (ER) architecture in hepatocytes, characterized by the induction of single rough ER sheet around the mitochondria, which becomes larger and flatter. These alterations are enriched in periportal and mid-lobular hepatocytes but not in pericentral hepatocytes. Gain- and loss-of-function in vivo models demonstrate that the Ribosome receptor binding protein1 (RRBP1) is required to enable fasting-induced ER sheet-mitochondria interactions and to regulate hepatic fatty acid oxidation. Endogenous RRBP1 is enriched around periportal and mid-lobular regions of the liver. In obesity, ER-mitochondria interactions are distinct and fasting fails to induce rough ER sheet-mitochondrion interactions. These findings illustrate the importance of a regulated molecular architecture for hepatocyte metabolic flexibility.

La evaluación de la política monetaria sobre la actividad económica se ha enfocado al ámbito nacional sin considerar sus efectos a nivel regional. El objetivo de este documento es estimar los efectos que un incremento no previsto de la tasa de interés tendría sobre la producción regional en México para el periodo 2000-2019. Para ello, se emplea un modelo estructural de vectores autorregresivos (SVAR) en panel. Los resultados sugieren un efecto negativo y diferenciado sobre la producción regional. Este efecto se presenta a partir del segundo trimestre, después del aumento de la tasa de interés.

Food insecurity among college students is an emerging public health issue, affecting a considera­ble proportion of the student population nation­wide, approximately 35–45%. Research is discover­ing links between college student food insecurity and physical and mental health, as well as academic performance. Such high prevalence of student food insecurity highlights the urgency of addressing the lack of consistent access to nutritious food. This research examines a pilot intervention at an urban public university that deployed miniature food pan­tries across campus from which anyone could take food anonymously. The research team systemati­cally restocked these pantries with food on a weekly basis for nearly two school years. Sensors installed in the pantries collected instances when individuals “interacted” with the pantry’s door. The sensor system documented thousands of interac­tions with the pantries each school year. As such, the intervention can be considered a success. How­ever, the miniature pantry model was not without flaws: its decentralized nature created challenges for the research team, the sensor system was often unstable, and heavy reliance on undergraduate stu­dents proved a long-term problem. The research team believes that administrative and information technology improvements could further enhance the model’s ability to mitigate campus food insecu­rity. This intervention could be an inspiration to other campuses and other institutions considering similar strategies.

El presente artículo analiza tres tipos de predicación no verbal (PNV) del dominio de la locación: locativas, existenciales y posesivas, de la lengua triqui (otomangue, familia mixtecana) hablada en el Poblado Miguel Alemán, Sonora, México (TPMA); con tendencia a expresarse tipológicamente de manera similar. Este persigue los siguientes objetivos: (i) definir la codificación de las PNV locativas, (ii) evidenciar los diversos mecanismos de enunciación de estas y, (iii) presentar sus similitudes y diferencias. Las PNV son unidades de análisis semántico y morfosintáctico (Hengeveld, 1992). Los datos son de tipo cualitativo provenientes de textos discursivos y elicitaciones, recopilados entre el 2019 y el 2023 en el Poblado Miguel Alemán, Sonora, México; pertenecientes a mis colaboradoras: Rosa y Hortencia Vázquez. En el análisis se obtuvo que las PNV locativas y existenciales se expresan con cópula cero y tres cópulas verbales. En contraste, las PNV posesivas se dividen en: genitivas, codificadas con la cópula me3, y de tener, emitida con la cópula vaa32. En TPMA, todas las PNV negativas documentadas se presentan con vaa32, excepto las de posesión genitiva; la cual cuenta con características particulares.

For young women, im/migration can impact education, employment, and family dynamics in ways that shape the courses of their lives and health. The purpose of this study is to deepen understandings of how im/migration shapes young women's experiences of pregnancy, motherhood, and marriage. Guided by intersectionality theory and the structural and social determinants of health, we analyzed qualitative interviews conducted with young im/migrants ages 15-30, who identify as women, and who had experiences of pregnancy, motherhood, and/or marriage (N ​= ​34). Participants were interviewed as part of the IRIS study in British Columbia, Canada between May 2019 to February 2021. Young im/migrant women described how im/migration shaped experiences of pregnancy, motherhood, and marriage by way of health, social, legal, and economic drivers. These included being ineligible for health insurance and unable to prevent pregnancy and experiencing workplace discrimination in form of reduced hours and no maternity benefits. Demanding caregiving roles and unsafe home environments were also described as being exacerbated by unaffordable childcare in destination settings and unequal gender roles and power dynamics after im/migration. Our findings suggest structural changes necessary to improve young im/migrant women's health. We recommend that health systems decouple im/migration status from health insurance; that governments provide all people with accessible childcare and maternity benefits; and that governments fund community-based efforts towards dismantling structures of patriarchy.

El objetivo de la presente revisión es analizar la literatura relacionada el estrés y sus fuentes generadoras en la aviación, un tema poco estudiado en Panamá. Además, se el trabajo busca describir las medidas que se están implementando en la aviación para disminuir los errores humanos en los accidentes aéreos. La presente revisión se realizó desde diciembre 2021 a enero 2022. Se consultaron las bases de datos MEDLINE, Google Scholar y Scielo, empleando descriptores clave como estrés, aviación, salud ocupacional, accidentes aéreos, entre otros. La revisión arrojó hallazgos relacionados con el estrés laboral como problema de salud creciente en los países en vías de desarrollo. Las profesiones relacionadas con la aviación tienden a sufrir de estrés laboral y otras afecciones psicológicas, debido a múltiples factores como la presión laboral, la ansiedad y aquellos relacionados con la responsabilidad por el trabajo desempeñado. Las organizaciones internacionales de aviación han implementado protocolos detallados para tratar de disminuir el factor humano dentro de los accidentes aéreos. El protocolo más utilizado es Crew Resource Management, que contribuye a la adecuada utilización de las máquinas y el recurso humano. En conclusión, las responsabilidades de los profesionales de la aviación, no solo consigo mismos y con la institución para la cual laboran, sino también con los pasajeros que transportan, les producen un aumento de estrés. Por lo que resulta importante brindar a estos profesionales terapias psicológicas efectivas para que reconozcan y puedan disminuir sus niveles de estrés. Además, se requieren abordaje desde las políticas públicas de salud ocupacional para tratar el estrés laboral, sobre todo en actividades de alto riesgo como la aviación.

El objetivo de este documento es analizar la relación entre el ciclo económico y diversas variables monetarias y financieras con el fin de determinar si han sido estables en el tiempo, teniendo en cuenta los efectos de las modificaciones en las condiciones económicas generales, la estrategia específica adoptada de política monetaria en diferentes subperiodos y las condiciones institucionales en que esta se maneja. Para probar la estabilidad de estas relaciones, una vez que se obtienen los indicadores del ciclo mediante los filtros convencionales, se emplea la metodología de cambio estructural de Bai y Perron (1998). Los resultados destacan dos cambios estructurales en la relación entre el indicador del ciclo y las variables de inflación, tasa de interés, tipo de cambio nominal y agregados monetarios nominales, uno a mediados de los años ochenta y el otro a mediados de los noventa. Abstract This paper aims to analyze the relationship between the business cycle and various monetary and financial variables. In particular, the paper seeks to determine whether this relationship has been stable over time, considering the effects of the changes in the general economic conditions, the specific monetary policy strategy adopted in different subperiods, and the institutional framework in which this is managed. To test the stability of these relationships, once the cycle indicators are obtained using conventional filters, the structural change methodology of Bai and Perron (1998) is used. The results highlight two structural changes in the relationship between the cycle indicator and inflation, interest rate, nominal exchange rate, and nominal monetary aggregates, one in the mid-eighties and the other in the middle of the nineties.

El potencial de habitabilidad térmica y el diseño de espacios públicos exteriores, establecen las condiciones de ambiente térmico de sus habitantes por la interrelación de sus componentes (Sevilla, 2008). En la ciudad de El Grullo, Jalisco, México, se considera que condiciones de clima cálido semiseco en periodo de verano, reduce niveles de habitabilidad térmica en espacios de convivencia públicos exteriores, revisión de literatura hasta 2019, indica que estudios sobre habitabilidad térmica en México, han sido desarrollados básicamente en vivienda y edificación, parte de lo que justifica la elaboración de este estudio. El objetivo de esta investigación es presentar el proceso de desarrollo de un Índice de Habitabilidad Térmica (IHT) para espacios de convivencia públicos exteriores, a partir de la estimación del efecto de variables físicas, meteorológicas y termo fisiológicas del habitante, en periodo frío. El caso de estudio fue la población de El Grullo y se utilizó una metodología descriptiva, no experimental y transversal. Después del análisis documental y necesidades del estudio, se adecuó la Fórmula de Confort (COMFA) desarrollada por Brown y Gillespie (1995). De acuerdo al diagnóstico bioclimático de horas de confort para El Grullo y el análisis de áreas de estudio, se aplicaron 554 cédulas de información de habitante y 98 de ambiente térmico y contexto urbano para periodo frío. Para alcanzar valores del IHT, se evaluó nivel de desempeño de cada indicador en función de complejidad, relevancia y accesibilidad de información, para análisis de datos se utilizó el coeficiente de correlación de Spearman y con base en los resultados obtenidos, se determina que el desarrollo del IHT contribuye a la relación y adecuación entre el hombre y su entorno, y es referido a la integración que resulta de la capacidad de satisfacer las necesidades humanas y sus expectativas de desarrollo y convivencia en un espacio público exterior, además el IHT funciona como herramienta de diseño urbano y sistema de evaluación como propuesta para mejorar las condiciones de confort y de habitabilidad de dichos espacios.

This study aims to formulate a regional food security model based on the relationship between characteristics of the area of production, consumption and entrepreneurship with the performance of community food business development (PUPM) in the Province of East Nusa Tenggara (NTT). The research sample consisted of 93 rice farmers, 96 rice consumers and 30 business people representing the Association of Farmers' Groups (Gapoktan) and the Indonesian Farmers Shop (TTI) owners, accounting for the total respondents to 219 people. Data analysis was using Smart-PLS. The results of the analysis show that the performance of the PUPM in NTT which is influenced by the characteristics of the production, consumption and entrepreneurship areas has only been achieved at a sufficient level. The analysis also shows that the new PUPM activities in NTT are able to fulfill aspects of access and stability, while the availability aspect is still very small. Nevertheless, this study has proven that the regional food security model in NTT can be realized through community food business development activities.

El objetivo de la investigación es identificar el crecimiento de la oferta turística en la Ruta del Vino de Baja California (México), una de las regiones vinícolas emergentes con gran potencial, donde se produce la mayoría del vino del país. Actualmente, es visitada por turistas nacionales e internacionales por la calidad de su vino y por sus atractivos singulares, como su paisaje, historia, cultura y oferta, que le dan una identidad única. El estudio se sustenta en la investigación documental, revisión bibliográfica y en fuentes secundarias, donde se identifica un importante crecimiento del sector vitivinícola, pasando de ser 16 vinícolas en el año 2004 a 89 vinícolas en el actual. Asimismo, el crecimiento vitivinícola ha influido en un incremento paralelo de la oferta turística en la región. Este rápido crecimiento, aunado al incremento del turismo, implica retos para la competitividad y la sostenibilidad de la región.

Objectives: Loneliness is a biopsychosocial determinant of health and contributes to physical and psychological chronic illnesses, functional decline, and mortality in older adults. This paper presents the results of the first randomized trial of LISTEN, which is a new cognitive behavioral intervention for loneliness, on loneliness, neuroimmunological stress response, psychosocial functioning, quality of life, and measures of physical health. Methods: The effectiveness of LISTEN was evaluated in a sample population comprising 27 lonely, chronically ill, older adults living in Appalachia. Participants were randomized into LISTEN or educational attention control groups. Outcome measures included salivary cortisol and DHEA, interleukin-6, interleukin-2, depressive symptoms, loneliness, perceived social support, functional ability, quality of life, fasting glucose, blood pressure, and body mass index. Results: At 12 weeks after the last intervention session, participants of the LISTEN group reported reduced loneliness (p = 0.03), enhanced overall social support (p = 0.05), and decreased systolic blood pressure (p = 0.02). The attention control group reported decreased functional ability (p = 0.10) and reduced quality of life (p = 0.13). Conclusions: LISTEN can effectively diminish loneliness and decrease the systolic blood pressure in community-dwelling, chronically ill, older adults. Results indicate that this population, if left with untreated loneliness, may experience functional impairment over a period as short as 4 months. Further studies on LISTEN are needed with larger samples, in varied populations, and over longer periods of time to assess the long-term effects of diminishing loneliness in multiple chronic conditions.

Este trabajo tiene como objetivo analizar, desde la perspectiva de género, la percepción que un grupo de mujeres de un poblado rururbano, con alta marginalidad, tienen de los espacios públicos y del contexto de sus viviendas. La investigación forma parte del proyecto Vivienda y mujer: espacios habitables para el desarrollo de familias con jefatura femenina en la comunidad de Flor de Coco, Municipio de Armería, Colima. Para el estudio se empleó una metodología cuali-cuantitativa con información de campo, entrevistas, talleres y el análisis del entorno inmediato de ocho viviendas con jefatura femenina. Los resultados muestran que existen diez tipos de inseguridad, desde la percepción de las mujeres: drogadicción, alcoholismo, robos y asaltos, violencia de género, pandillerismo, inseguridad por falta de iluminación, por falta de vigilancia, por riesgo de deslaves, por problemas de movilidad y por inundaciones y crecidas del río. Como señalan Nieto Morales y Esquivel Hernández, Soto Villagrán, Ortiz Escalante, Col-lectiu Punt 6 y Farapi, la ausencia de una planeación con perspectiva de género de la forma urbana y su espacio público afecta más a las mujeres, lo que las vuelve más vulnerables.

Esta investigación evalúa el poder predictivo de una familia de modelos GARCH usados en la predicción de la volatilidad de los rendimientos de la Mezcla Mexicana de Exportación durante el periodo del 2 de enero de 1989 al 30 de diciembre de 2011. Los resultados empíricos evidencian un alto grado de persistencia y la presencia de efectos asimétricos en la volatilidad. Aunque la prueba de predicción sesgada muestra que el modelo IGARCH proporciona el mejor ajuste para recoger la persistencia infinita de los choques en la volatilidad, estos hallazgos no son sustentados por las robustas funciones de pérdidas y la prueba estadística de Diebold-Mariano, debido a que los modelos GARCH y EGARCH proporcionan mejores predicciones de la volatilidad fuera de muestra para los horizontes de 1 y 5 días.

En este documento se evalúan los choques de precios de recursos naturales, asícomo el rol de la asignación de los ingresos fiscales sobre la posición fiscal de Bolivia, país dependiente de gas natural con base en información trimestral: 2003-2011 (período de auge de precios). Al emplear la metodología SVAR, se ejemplifica −con un caso− al demostrar que la asignación de ingresos fiscales al gasto público ejerce un rol más relevante que las perturbaciones de precios en el recurso natural para distintas mediciones de balances fiscales; asimismo, se evalúan las respuestas del gasto público desagregado en términos de presión fiscal.

Introduction: An abdominal aortic aneurysm (AAA) is a dilation localized in the infrarenal segment of the abdominal aorta that can expand continuously and rupture if left untreated. Computational methods such as finite element analysis (FEA) are widely used with in silico models to calculate biomechanical predictors of rupture risk while choosing constitutive material properties for the AAA wall and intraluminal thrombus (ILT)., Methods: In the present work, we investigated the effect of different constitutive material properties for the wall and ILT on 21 idealized and 10 unruptured patient-specific AAA geometries. Three material properties were used to characterize the wall and two for the ILT, leading to six material model combinations for each AAA geometry subject to appropriate boundary conditions., Results: The results of the FEA simulations indicate significant differences in the average peak wall stress (PWS), 99th percentile wall stress (99th WS), and spatially averaged wall stress (SAWS) for all AAA geometries subject to the choice of a material model combination. Specifically, using a material model combination with a compliant ILT yielded statistically higher wall stresses compared to using a stiff ILT, irrespective of the constitutive equation used to model the AAA wall., Discussion: This work provides quantitative insight into the variability of the wall stress distributions ensuing from AAA FEA modeling due to its strong dependency on population-averaged soft tissue material characterizations. This dependency leads to uncertainty about the true biomechanical state of stress of an individual AAA and the subsequent assessment of its rupture risk., (© 2024. The Author(s) under exclusive licence to Biomedical Engineering Society.)

Plant pathogenic bacteria often have a narrow host range, which can vary among different isolates within a population. Here, we investigated the host range of the tomato pathogen Clavibacter michiganensis (Cm). We determined the genome sequences of 40 tomato Cm isolates and screened them for pathogenicity on tomato and eggplant. Our screen revealed that out of the tested isolates, five were unable to cause disease on any of the hosts, 33 were exclusively pathogenic on tomato, and two were capable of infecting both tomato and eggplant. Through comparative genomic analyses, we identified that the five non-pathogenic isolates lacked the chp/tomA pathogenicity island, which has previously been associated with virulence in tomato. In addition, we found that the two eggplant-pathogenic isolates encode a unique allelic variant of the putative serine hydrolase chpG (chpGC), an effector that is recognized in eggplant. Introduction of chpGC into a chpG inactivation mutant in the eggplant-non-pathogenic strain Cm101, failed to complement the mutant, which retained its ability to cause disease in eggplant and failed to elicit hypersensitive response (HR). Conversely, introduction of the chpG variant from Cm101 into an eggplant pathogenic Cm isolate (C48), eliminated its pathogenicity on eggplant, and enabled C48 to elicit HR. Our study demonstrates that allelic variation in the chpG effector gene is a key determinant of host range plasticity within Cm populations., Competing Interests: The authors declare that no competing interests exist., (Copyright: © 2024 Verma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Chemistry in eukaryotic intercellular spaces is shaped by both hosts and symbiotic microorganisms such as bacteria. Pathogenic microorganisms like barley-associated Xanthomonas translucens (Xt) swiftly overtake the inner leaf tissue becoming the dominant microbial community member during disease development. The dynamic metabolic changes due to Xt pathogenesis in the mesophyll spaces remain unknown. Genomic group I of Xt consists of two barley-infecting lineages: pathovar translucens (Xtt) and pathovar undulosa (Xtu). Xtu and Xtt, although genomically distinct, cause similar water-soaked lesions. To define the metabolic signals associated with inner leaf colonization, we used untargeted metabolomics to characterize Xtu and Xtt metabolism signatures associated with mesophyll growth. We found that mesophyll apoplast fluid from infected tissue yielded a distinct metabolic profile and shift from catabolic to anabolic processes over time compared to water-infiltrated control. The pathways with the most differentially expressed metabolites by time were glycolysis, tricarboxylic acid cycle, sucrose metabolism, pentose interconversion, amino acids, galactose, and purine metabolism. Hierarchical clustering and principal component analysis showed that metabolic changes were more affected by the time point rather than the individual colonization of the inner leaves by Xtt compared to Xtu. Overall, in this study, we identified metabolic pathways that explain carbon and nitrogen usage during host-bacterial interactions over time for mesophyll tissue colonization. This foundational research provides initial insights into shared metabolic strategies of inner leaf colonization niche occupation by related but phylogenetically distinct phyllosphere bacteria., Importance: The phyllosphere is a habitat for microorganisms including pathogenic bacteria. Metabolic shifts in the inner leaf spaces for most plant-microbe interactions are unknown, especially for Xanthomonas species in understudied plants like barley ( Hordeum vulgare ). Xanthomonas translucens pv. translucens (Xtt) and Xanthomonas translucens pv. undulosa (Xtu) are phylogenomically distinct, but both colonize barley leaves for pathogenesis. In this study, we used untargeted metabolomics to shed light on Xtu and Xtt metabolic signatures. Our findings revealed a dynamic metabolic landscape that changes over time, rather than exhibiting a pattern associated with individual pathovars. These results provide initial insights into the metabolic mechanisms of X. translucens inner leaf pathogenesis., Competing Interests: The authors declare no conflict of interest.

Quickly understanding the genomic changes that lead to pathogen emergence is necessary to launch mitigation efforts and reduce harm. In this study, we tracked in real time a 2022 bacterial plant disease outbreak in U.S. geraniums ( Pelargonium × hortorum ) caused by Xhp2022, a novel lineage of Xanthomonas hortorum . Genomes from 31 Xhp2022 isolates from seven states showed limited chromosomal variation and all contained a single plasmid (p93). Time tree and single nucleotide polymorphism whole-genome analysis estimated that Xhp2022 emerged within the last decade. The phylogenomic analysis determined that p93 resulted from the cointegration of three plasmids (p31, p45, and p66) found sporadically across isolates from previous outbreaks. Although p93 had a 49 kb nucleotide reduction, it retained putative fitness genes, which became predominant in the 2022 outbreak. Overall, we demonstrated, through rapid whole-genome sequencing and analysis, a recent, traceable event of genome reduction for niche adaptation typically observed over millennia in obligate and fastidious pathogens.IMPORTANCEThe geranium industry, valued at $4 million annually, faces an ongoing Xanthomonas hortorum pv. pelargonii (Xhp) pathogen outbreak. To track and describe the outbreak, we compared the genome structure across historical and globally distributed isolates. Our research revealed Xhp population has not had chromosome rearrangements since 1974 and has three distinct plasmids. In 2012, we found all three plasmids in individual Xhp isolates. However, in 2022, the three plasmids co-integrated into one plasmid named p93. p93 retained putative fitness genes but lost extraneous genomic material. Our findings show that the 2022 strain group of the bacterial plant pathogen Xanthomonas hortorum underwent a plasmid reduction. We also observed several Xanthomonas species from different years, hosts, and continents have similar plasmids to p93, possibly due to shared agricultural settings. We noticed parallels between genome efficiency and reduction that we see across millennia with obligate parasites with increased niche specificity., Competing Interests: The authors declare no conflict of interest.

Background: Xanthomonas translucens pv. graminis (Xtg) is a major bacterial pathogen of economically important forage grasses, causing severe yield losses. So far, genomic resources for this pathovar consisted mostly of draft genome sequences, and only one complete genome sequence was available, preventing comprehensive comparative genomic analyses. Such comparative analyses are essential in understanding the mechanisms involved in the virulence of pathogens and to identify virulence factors involved in pathogenicity., Results: In this study, we produced high-quality, complete genome sequences of four strains of Xtg, complementing the recently obtained complete genome sequence of the Xtg pathotype strain. These genomic resources allowed for a comprehensive comparative analysis, which revealed a high genomic plasticity with many chromosomal rearrangements, although the strains were highly related. A high number of transposases were exclusively found in Xtg and corresponded to 413 to 457 insertion/excision transposable elements per strain. These mobile genetic elements are likely to be involved in the observed genomic plasticity and may play an important role in the adaptation of Xtg. The pathovar was found to lack a type IV secretion system, and it possessed the smallest set of type III effectors in the species. However, three XopE and XopX family effectors were found, while in the other pathovars of the species two or less were present. Additional genes that were specific to the pathovar were identified, including a unique set of minor pilins of the type IV pilus, 17 TonB-dependent receptors (TBDRs), and 11 plant cell wall degradative enzymes., Conclusion: These results suggest a high adaptability of Xtg, conferred by the abundance of mobile genetic elements, which could play a crucial role in pathogen adaptation. The large amount of such elements in Xtg compared to other pathovars of the species could, at least partially, explain its high virulence and broad host range. Conserved features that were specific to Xtg were identified, and further investigation will help to determine genes that are essential to pathogenicity and host adaptation of Xtg., (© 2023. The Author(s).)

Bacterial leaf streak, bacterial blight, and black chaff caused by Xanthomonas translucens pathovars are major diseases affecting small grains. Xanthomonas translucens pv. translucens and X. translucens pv. undulosa are seedborne pathogens that cause similar symptoms on barley, but only X. translucens pv. undulosa causes bacterial leaf streak of wheat. Recent outbreaks of X. translucens have been a concern for wheat and barley growers in the Northern Great Plains; however, there are limited diagnostic tools for pathovar differentiation. We developed a multiplex PCR based on whole-genome differences to distinguish X. translucens pv. translucens and X. translucens pv. undulosa . We validated the primers across different Xanthomonas and non- Xanthomonas strains. To our knowledge, this is the first multiplex PCR to distinguish X. translucens pv. translucens and X. translucens pv. undulosa . These molecular tools will support disease management strategies enabling detection and pathovar incidence analysis of X. translucens . [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license., Competing Interests: The author(s) declare no conflict of interest.

Bacterial leaf streak (BLS) is a disease of monocot plants caused by Xanthomonas translucens on small grains, X. vasicola on maize and sorghum, and X. oryzae on rice. These three pathogens cause remarkably similar symptomology in their host plants. Despite causing similar symptoms, BLS pathogens are dispersed throughout the larger Xanthomonas phylogeny. Each aforementioned species includes strain groups that do not cause BLS and instead cause vascular disease. In this commentary, we hypothesize that strains of X. translucens , X. vasicola , and X. oryzae convergently evolved to cause BLS due to shared evolutionary pressures. We examined the diversity of secreted effectors, which may be important virulence factors for BLS pathogens and their evolution. We discuss evidence that differences in gene regulation and abilities to manipulate plant hormones may also separate BLS pathogens from other Xanthomonas species or pathovars. BLS is becoming an increasing issue across the three pathosystems. Overall, we hope that a better understanding of conserved mechanisms used by BLS pathogens will enable researchers to translate findings across production systems and guide approaches to control this (re)emerging threat., Competing Interests: The author(s) declare no conflict of interest.

Bacterial leaf streak (BLS) of wheat ( Triticum aestivum ), caused by Xanthomonas translucens pv. undulosa , is a disease of major concern in the Northern Great Plains. The host range for X. translucens pv. undulosa is relatively broad, including several small grains and perennial grasses. In Minnesota, X. translucens pv. undulosa was isolated from weedy grasses in and around wheat fields that exhibited BLS symptoms and from cultivated wild rice ( Zizania palustris ) with symptomatic leaf tissue. Currently, no genomic resources are available for X. translucens pv. undulosa strains isolated from non-wheat hosts. In this study, we sequenced and assembled the complete genomes of five strains isolated from weedy grass hosts, foxtail barley ( Hordeum jubatum ), green foxtail ( Setaria viridis ), and wild oat ( Avena fatua ), and from cultivated wild rice and wheat. These five genomes were compared with the publicly available genomes of seven X. translucens pv. undulosa strains originating from wheat and one genome of an X. translucens pv. secalis strain originating from rye ( Secale cereale ). Global alignments of the genomes revealed little variation in genomic structures. Average nucleotide identity-based phylogeny and life identification numbers revealed that the strains share ≥99.25% identity. We noted differences in the presence of Type III secreted effectors, including transcription activator-like effectors. Despite differences between strains, we did not identify unique features distinguishing strains isolated from wheat and non-wheat hosts. This study contributes to the availability of genomic data for X. translucens pv. undulosa from non-wheat hosts, thus increasing our understanding of the diversity within the pathogen population., Competing Interests: The author(s) declare no conflict of interest.

Xanthomonas translucens pv. translucens (Xtt) is a global barley patho-gen and a concern for resistance breeding and regulation. Long-read whole genome sequences allow in-depth understanding of pathogen diversity. We have completed long-read PacBio sequencing of two Minnesotan Xtt strains and an in-depth analysis of available Xtt genomes. We found that average nucleotide identity (ANI)-based approaches organize Xtt strains different from the previous standard multilocus sequencing analysis approach. According to ANI, Xtt forms a separate clade from X. translucens pv. undulosa and consists of three main groups which are represented on multiple continents. Some virulence factors, such as 17 Type III-secreted effectors, are highly conserved and offer potential targets for the elicitation of broad resistance. However, there is a high degree of variation in virulence factors, meaning that germplasm should be screened for resistance with a diverse panel of Xtt., Competing Interests: The author(s) declare no conflict of interest.

Strains of Xanthomonas citri pv. malvacearum cause bacterial blight of cotton, a potentially serious threat to cotton production worldwide, including in sub-Saharan countries. Development of disease symptoms, such as water soaking, has been linked to the activity of a class of type 3 effectors, called transcription activator-like (TAL) effectors, which induce susceptibility genes in the host's cells. To gain further insight into the global diversity of the pathogen, to elucidate their repertoires of TAL effector genes, and to better understand the evolution of these genes in the cotton-pathogenic xanthomonads, we sequenced the genomes of three African strains of X. citri pv. malvacearum using nanopore technology. We show that the cotton-pathogenic pathovar of X. citri is a monophyletic lineage containing at least three distinct genetic subclades, which appear to be mirrored by their repertoires of TAL effectors. We observed an atypical level of TAL effector gene pseudogenization, which might be related to resistance genes that are deployed to control the disease. Our work thus contributes to a better understanding of the conservation and importance of TAL effectors in the interaction with the host plant, which can inform strategies for improving resistance against bacterial blight in cotton., Competing Interests: The author(s) declare no conflict of interest.

Evolutionarily, early-branching xanthomonads, also referred to as clade-1 xanthomonads, include major plant pathogens, most of which colonize monocotyledonous plants. Seven species have been validly described, among them the two sugarcane pathogens Xanthomonas albilineans and Xanthomonas sacchari , as well as Xanthomonas translucens , which infects small-grain cereals and diverse grasses but also asparagus and pistachio trees. Single-gene sequencing and genomic approaches have indicated that this clade likely contains more, yet-undescribed species. In this study, we sequenced representative strains of three novel species using long-read sequencing technology. Xanthomonas campestris pv. phormiicola strain CFBP 8444 causes bacterial streak on New Zealand flax, another monocotyledonous plant. Xanthomonas sp. strain CFBP 8443 has been isolated from common bean, and Xanthomonas sp. strain CFBP 8445 originated from banana. Complete assemblies of the chromosomes confirmed their unique phylogenetic position within clade 1 of Xanthomonas . Genome mining revealed novel genetic features, hitherto undescribed in other members of the Xanthomonas genus. In strain CFBP 8444, we identified genes related to the synthesis of coronatine-like compounds, a phytotoxin produced by several pseudomonads, which raises interesting questions about the evolution and pathogenicity of this pathogen. Furthermore, strain CFBP 8444 was found to contain a second, atypical flagellar gene cluster in addition to the canonical flagellar gene cluster. Overall, this research represents an important step toward better understanding the evolutionary history and biology of early-branching xanthomonads., Competing Interests: The author(s) declare no conflict of interest.

Winterberries ( Ilex verticillata and hybrids) are deciduous species of holly whose branches bearing colorful fruit are cut in late Fall to be used for seasonal decorations. The annual wholesale value of the woody cuts is $1.5 million nationally (NASS, 2019). In June 2021, approximately 80% of the 45 Ilex verticillata 'Maryland Beauty' potted plants, which were maintained in a container yard at The Ohio State University research farm in Columbus, OH, presented leaves with irregular necrotic lesions surrounded by a chlorotic halo. No other symptoms were present on the plants. Bacterial streaming was observed from the lesions using a compound microscope and isolations were performed after surface disinfesting small sections of leaf tissue from the border of the lesions by soaking in 10% bleach for 30 sec, rinsing twice in sterile water, macerating in sterile water, and streaking the suspension on nutrient broth yeast extract agar. Creamy white, circular, smooth, and convex colonies were recovered after incubation at 28°C for 48 h. Bacterial identification of one representative isolate was initially pursued from single colonies of a purified culture using five discriminative phenotypic tests (i.e., LOPAT: "L", levan production; "O", oxidase activity; "P", pectinolytic activity; "A", arginine dehydrolase production; "T", tobacco hypersensitive reaction), which resulted in the L+ O- P- A- T+ profile consistent with the description of Pseudomonas syringae (Lelliott et al. 1996). Molecular identification was performed based on rpoD marker amplification and sequencing using primers PsrpoD FNP1/PsrpoDnprpcr1 (Parkison et al. 2011). NCBI GenBank BLASTn comparison of the rpoD sequence (GenBank Acc. No. OP221440) shared 99.12% identity to P. syringae pv. passiflorae (AB163366.1). Whole genome sequence analysis was conducted to strengthen the classification of the isolate species. To this extent, DNA was sequenced with an iSeq 100 Illumina benchtop sequencer using Illumina DNA Prep kit and iSeq 100 i1 Reagent v2 (Illumina, Inc, REF: 20060060 and 20031371). Illumina Local Run Manager software was used for base calling, demultiplexing, and trimming of the raw reads. Unicycler v0.5.0 was used for de novo assembly of the genome (Wick et al. 2017). The assembled genome size was 5.9 Mb with 959 contigs and 10× coverage (NCBI GenBank Biosample No. SAMN30281368; Acc. No. JANQCB010000000). Average nucleotide identity (ANI) analysis was performed on the server MiGA online (Rodriguez-R et al. 2018). Subgroup identification was inconclusive ( p >0.05), positioning this isolate between P. syringae pv. actinidiae (96.45% ANI) and pv. viburni (96.65% ANI) (Rodriguez-R & Konstantinidis, 2016). Both these pathovars cause leaf spots on woody plants such as kiwi and viburnum (Donati et al. 2020; Garibaldi et al. 2005). To confirm pathogenicity, three separate branches on each of two I. verticillata 'Maryland Beauty' potted plants were selected, and 5-7 individual young leaves (>2 weeks from emergence) on each branch were infiltrated with a bacterial suspension (10 8 CFU/mL) in sterile water (SW) using a needleless syringe by delivering 30-50 µL of suspension per infiltration point. One additional branch per plant was infiltrated with SW to serve as control. Plants were covered with a plastic bag for two days post-inoculation (DPI) and maintained in the laboratory at an average of 23°C. All inoculated leaves showed necrotic lesions two DPI while control leaves remained asymptomatic. To fulfill Koch's postulates, the bacterium was re-isolated from the symptomatic leaves six DPI and confirmed to be identical to the original isolate based on rpoD gene sequencing. To the best of our knowledge, this report signifies the first instance of P. syringae causing bacterial leaf spot on winterberry worldwide. Ornamental plant sales are based primarily on visual appeal; therefore, identification and monitoring of emerging pathogens is essential to ensure the health of the industry.

Changes in Xanthomonas race and species composition causing bacterial spot of tomato have occurred throughout the world and are often associated with epidemics. Knowledge of bacterial population structure is key for resistance discovery and deployment. We surveyed Xanthomonas spp. composition from processing tomato fields in the Midwestern United States over a 4-year period between 2017 and 2020, compared these to strains collected previously, and found that X. perforans is currently the most prevalent species. We characterized 564 X. perforans isolates for sequence variation in avrXv3 to distinguish between race T3 and T4 and validated race designation using hypersensitive response (HR) assays for 106 isolates. Race T4 accounted for over 95% of X. perforans isolates collected in the Midwest between 2017 and 2020. Whole genome sequencing, Average Nucleotide Identity (ANI) analysis, core genome alignment and single nucleotide polymorphism (SNP) detection relative to a reference strain, and phylogenomic analysis suggest that the majority of Midwestern X. perforans strains collected between 2017 and 2020 were nearly identical, with greater than 99.99% ANI to X. perforans isolates collected from Collier County, Florida in 2012. These isolates shared a common SNP variant resulting an a premature stop codon in avrXv3. One sequenced isolate was identified with a deletion of avrXv3 and shared 99.99% ANI with a strain collected in Collier Co., Florida in 2006. A population shift to X. perforans T4 occurred in the absence of widely deployed resistance, with only 7% of tomato varieties tested having the resistant allele at the Xv3 / Rx-4 locus. The persistence of nearly identical strains over multiple years suggests that migration led to the establishment of an endemic population. Our findings validate a genomics-based framework to track shifts in X. perforans populations due to migration, mutation, drift, or selection based on comparisons to 146 genomes., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Bernal, Rotondo, Roman-Reyna, Klass, Timilsina, Minsavage, Iruegas-Bocardo, Goss, Jones, Jacobs, Miller and Francis.)

The Xanthomonas translucens species comprises phytopathogenic bacteria that can cause serious damage to cereals and to forage grasses. So far, the genomic resources for X. translucens were limited, which hindered further understanding of the host-pathogen interactions at the molecular level and the development of disease-resistant cultivars. To this end, we complemented the available complete genome sequence of the X. translucens pv. translucens pathotype strain DSM 18974 by sequencing the genomes of all the other 10 X. translucens pathotype strains using PacBio long-read technology and assembled complete genome sequences. Phylogeny based on average nucleotide identity (ANI) revealed three distinct clades within the species, which we propose to classify as clades Xt-I, Xt-II, and Xt-III. In addition to 2,181 core X. translucens genes, a total of 190, 588, and 168 genes were found to be exclusive to each clade, respectively. Moreover, 29 non-transcription activator-like effector (TALE) and 21 TALE type III effector classes were found, and clade- or strain-specific effectors were identified. Further investigation of these genes could help to identify genes that are critically involved in pathogenicity and/or host adaptation, setting the grounds for the development of new resistant cultivars., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Goettelmann, Roman-Reyna, Cunnac, Jacobs, Bragard, Studer, Koebnik and Kölliker.)

Comprehensive meta-analyses of risky decision making in children, adolescents, and adults have revealed that age trends in disambiguated laboratory tasks confirmed fuzzy-trace theory's prediction that preference for risk decreases monotonically from childhood to adulthood. These findings are contrary to predictions of dual systems or neurobiological imbalance models. Assumptions about increasing developmental reliance on mental representations of the gist of risky options are essential to account for this developmental trend. However, dual systems theory appropriately emphasizes how cultural context changes behavioral manifestation of risk preferences across age and neurobiological imbalance models appropriately emphasize developmental changes in reward sensitivity. All of the major theories include the assumption of increasing behavioral inhibition. Here, we integrate these theoretical constructs-representation, cultural context, reward sensitivity, and behavioral inhibition-to provide a novel framework for understanding and improving risky decision making in youth. We also discuss the roles of critical tests, scientific falsification, disambiguating assessments of psychological and neurological processes, and the misuse of such concepts as ecological validity and reverse inference. We illustrate these concepts by extending fuzzy-trace theory to explain why youth are a major conduit of viral infections, including the virus that causes COVID-19. We conclude by encouraging behavioral scientists to embrace new ways of thinking about risky decision making that go beyond traditional stereotypes about adolescents and that go beyond conceptualizing ideal decision making as trading off degrees of risk and reward.

Xylella fastidiosa ( Xf ) is a globally distributed plant-pathogenic bacterium. The primary control strategy for Xf diseases is eradicating infected plants; therefore, timely and accurate detection is necessary to prevent crop losses and further pathogen dispersal. Conventional Xf diagnostics primarily relies on quantitative PCR (qPCR) assays. However, these methods do not consider new or emerging variants due to pathogen genetic recombination and sensitivity limitations. We developed and tested a metagenomics pipeline using in-house short-read sequencing as a complementary approach for affordable, fast, and highly accurate Xf detection. We used metagenomics to identify Xf to the strain level in single- and mixed-infected plant samples at concentrations as low as 1 pg of bacterial DNA per gram of tissue. We also tested naturally infected samples from various plant species originating from Europe and the United States. We identified Xf subspecies in samples previously considered inconclusive with real-time PCR (quantification cycle [ C q ], >35). Overall, we showed the versatility of the pipeline by using different plant hosts and DNA extraction methods. Our pipeline provides taxonomic and functional information for Xf diagnostics without extensive knowledge of the disease. This pipeline demonstrates that metagenomics can be used for early detection of Xf and incorporated as a tool to inform disease management strategies. IMPORTANCE Destructive Xylella fastidiosa ( Xf ) outbreaks in Europe highlight this pathogen's capacity to expand its host range and geographical distribution. The current disease diagnostic approaches are limited by a multiple-step process, biases to known sequences, and detection limits. We developed a low-cost, user-friendly metagenomic sequencing tool for Xf detection. In less than 3 days, we were able to identify Xf subspecies and strains in field-collected samples. Overall, our pipeline is a diagnostics tool that could be easily extended to other plant-pathogen interactions and implemented for emerging plant threat surveillance.