16 results on '"Reto Crameri"'
Search Results
2. IgE Sensitization Profiles Differ between Adult Patients with Severe and Moderate Atopic Dermatitis.
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Irene Mittermann, Gustav Wikberg, Catharina Johansson, Christian Lupinek, Lena Lundeberg, Reto Crameri, Rudolf Valenta, and Annika Scheynius
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Medicine ,Science - Abstract
BackgroundAtopic dermatitis (AD) is a complex chronic inflammatory disease where allergens can act as specific triggering factors.AimTo characterize the specificities of IgE-reactivity in patients with AD to a broad panel of exogenous allergens including microbial and human antigens.MethodologyAdult patients with AD were grouped according to the SCORAD index, into severe (n = 53) and moderate AD (n = 126). As controls 43 patients were included with seborrhoeic eczema and 97 individuals without history of allergy or skin diseases. Specific IgE reactivity was assessed in plasma using Phadiatop®, ImmunoCap™, micro-arrayed allergens, dot-blotted recombinant Malassezia sympodialis allergens, and immune-blotted microbial and human proteins.ResultsIgE reactivity was detected in 92% of patients with severe and 83% of patients with moderate AD. Sensitization to cat allergens occurred most frequently, followed by sensitization to birch pollen, grass pollen, and to the skin commensal yeast M. sympodialis. Patients with severe AD showed a significantly higher frequency of IgE reactivity to allergens like cat (rFel d 1) and house dust mite (rDer p 4 and 10), to Staphylococcus aureus, M. sympodialis, and to human antigens. In contrast, there were no significant differences in the frequencies of IgE reactivity to the grass pollen allergens rPhl p 1, 2, 5b, and 6 between the two AD groups. Furthermore the IgE reactivity profile of patients with severe AD was more spread towards several different allergen molecules as compared to patients with moderate AD.ConclusionWe have revealed a hitherto unknown difference regarding the molecular sensitization profile in patients with severe and moderate AD. Molecular profiling towards allergen components may provide a basis for future investigations aiming to explore the environmental, genetic and epigenetic factors which could be responsible for the different appearance and severity of disease phenotypes in AD.
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- 2016
- Full Text
- View/download PDF
3. Structural aspects of fungal allergens
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Reto Crameri, University of Zurich, and Crameri, Reto
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Models, Molecular ,2403 Immunology ,Antigens, Fungal ,Molecular Structure ,business.industry ,Intracellular protein ,Immunology ,Fungi ,610 Medicine & health ,Allergens ,Cross Reactions ,Biology ,3. Good health ,Biotechnology ,Biochemistry ,10183 Swiss Institute of Allergy and Asthma Research ,Hypersensitivity ,2723 Immunology and Allergy ,Humans ,Immunology and Allergy ,business - Abstract
Despite the increasing number of solved crystal structures of allergens, the key question why some proteins are allergenic and the vast majority is not remains unanswered. The situation is not different for fungal allergens which cover a wide variety of proteins with different chemical properties and biological functions. They cover enzymes, cell wall, secreted, and intracellular proteins which, except cross-reactive allergens, does not show any evidence for structural similarities at least at the three-dimensional level. However, from a diagnostic point of view, pure allergens biotechnologically produced by recombinant technology can provide us, in contrast to fungal extracts which are hardly producible as standardized reagents, with highly pure perfectly standardized diagnostic reagents.
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- 2021
4. Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer
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Claudio Rhyner, Annika Scheynius, Yvonne Resch-Marat, Lukas Einhorn, Hiroshi Matsuda, Gerlinde Hofstetter, Edmund K. Hainisch, Irene Mittermann, Isabella Pali-Schöll, Eliane Isabelle Marti, Susanne Vrtala, Reto Crameri, H Sato, I Fukuda, Akane Tanaka, Rie Satoh, Sabine Brandt, Kanichi Kusano, Reiko Teshima, Rudolf Valenta, Erika Jensen-Jarolim, University of Zurich, and Jensen-Jarolim, E
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Male ,0301 basic medicine ,Allergy ,Microarray ,Immunology ,610 Medicine & health ,component‐resolved diagnosis ,Immunoglobulin E ,medicine.disease_cause ,Allergic sensitization ,Epitopes ,03 medical and health sciences ,Allergen ,10183 Swiss Institute of Allergy and Asthma Research ,medicine ,Animals ,Humans ,Immunology and Allergy ,Horses ,molecular ,Sensitization ,Recurrent airway obstruction ,2403 Immunology ,ISAC ,biology ,business.industry ,Horse ,Allergens ,Antigens, Plant ,medicine.disease ,horse ,3. Good health ,030104 developmental biology ,medicine.anatomical_structure ,Experimental Allergy and Immunology ,2723 Immunology and Allergy ,biology.protein ,Female ,Original Article ,IgE ,ORIGINAL ARTICLES ,business ,microarray ,Epitope Mapping ,Fagopyrum ,allergen - Abstract
BACKGROUND Companion animals are also affected by IgE-mediated allergies, but the eliciting molecules are largely unknown. We aimed at refining an allergen microarray to explore sensitization in horses and compare it to the human IgE reactivity profiles. METHODS Custom-designed allergen microarray was produced on the basis of the ImmunoCAP ISAC technology containing 131 allergens. Sera from 51 horses derived from Europe or Japan were tested for specific IgE reactivity. The included horse patients were diagnosed for eczema due to insect bite hypersensitivity, chronic coughing, recurrent airway obstruction and urticaria or were clinically asymptomatic. RESULTS Horses showed individual IgE-binding patterns irrespective of their health status, indicating sensitization. In contrast to European and Japanese human sensitization patterns, frequently recognized allergens were Aln g 1 from alder and Cyn d 1 from Bermuda grass, likely due to specific respiratory exposure around paddocks and near the ground. The most prevalent allergen for 72.5% of the tested horses (37/51) was the 2S-albumin Fag e 2 from buckwheat, which recently gained importance not only in human but also in horse diet. CONCLUSION In line with the One Health concept, covering human health, animal health and environmental health, allergen microarrays provide novel information on the allergen sensitization patterns of the companion animals around us, which may form a basis for allergen-specific preventive and therapeutic concepts.
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- 2018
5. Structural aspects and clinical relevance of Aspergillus fumigatus antigens/allergens
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Reto Crameri, Andreas G. Glaser, Sabine Zeller, Michael Weichel, Andreas Limacher, and Claudio Rhyner
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biology ,cDNA library ,General Medicine ,biology.organism_classification ,Immunoglobulin E ,medicine.disease ,Epitope ,Aspergillus fumigatus ,Microbiology ,Infectious Diseases ,Antigen ,Ribosomal protein ,biology.protein ,medicine ,Antibody ,Allergic bronchopulmonary aspergillosis - Abstract
Robotics-based high throughput screening of Aspergillus fumigatus cDNA libraries displayed on phage surfaces revealed at last 81 different structures able to bind IgE from serum of patients sensitized to this fungus. Among these, species-specific as well as phylogenetically highly conserved structures and such with unknown function have been detected. A subset of cDNAs have been used to produce and characterize the corresponding recombinant allergens which have proven to be useful diagnostic reagents allowing specific detection of A. fumigatus sensitization and differential diagnosis of allergic bronchopulmonary aspergillosis. Phylogenetically highly conserved structures like manganese-dependent superoxide dismutase, P2 acidic ribosomal protein, cyclophilins and thioredoxins induce, beyond sensitization, IgE antibodies able to cross-react with the corresponding homologous self antigens. These reactions, likely to contribute to the exacerbation and perpetuation of allergic bronchopulmonary aspergillosis, can be traced back to shared conformational B-cell epitopes build up from conserved amino acid residues scattered over the surface of the molecules as shown by detailed analyses of the crystal structures.
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- 2018
6. Evanescence wave-based technology for the rapid and sensitive quantification of biological analytes
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Torsten Zuberbier, Reto Crameri, Gerald Quapil, Max Wiki, Claudio Rhyner, Manfred Schawaller, Cezmi A. Akdis, and University of Zurich
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Analyte ,2403 Immunology ,Chromatography ,business.industry ,Immunology ,Enzyme-Linked Immunosorbent Assay ,610 Medicine & health ,Biosensing Techniques ,030204 cardiovascular system & hematology ,Molecular biology ,03 medical and health sciences ,0302 clinical medicine ,030202 anesthesiology ,10183 Swiss Institute of Allergy and Asthma Research ,2723 Immunology and Allergy ,Humans ,Immunology and Allergy ,Medicine ,business - Published
- 2018
7. Aspergillus fumigatus-specific immunoglobulin levels in BALF of CF patients
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Zsolt Szépfalusi, Petra Fucik, Birgit Pfaller, Sabine Renner, Markus Debiasi, Klara Schmidthaler, Jonathan Argeny, Thomas Eiwegger, Saskia Gruber, Claudio Rhyner, Mia Goña-Höpler, Andreas G. Glaser, Reto Crameri, Edith Nachbaur, Stefan Kanolzer, and University of Zurich
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0301 basic medicine ,Pulmonary and Respiratory Medicine ,Immunoglobulin levels ,biology ,business.industry ,lcsh:R ,Original Research Letter ,lcsh:Medicine ,610 Medicine & health ,respiratory system ,biology.organism_classification ,3. Good health ,Aspergillus fumigatus ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030228 respiratory system ,10183 Swiss Institute of Allergy and Asthma Research ,otorhinolaryngologic diseases ,Medicine ,business - Abstract
A lack of correlation between systemic and local IgE production at mucosal sites has been reported for allergic asthma [1–5], allergic rhinitis [6, 7] and chronic rhinosinusitis with nasal polyps [8–10]. In allergic asthmatics, local IgE production is higher than in nonallergic asthmatics [3] and high local IgE levels have been linked to the clinical phenotype. Interestingly, in cases of nasal polyps, local IgE targets mainly superantigens [8]., IgE responses to Aspergillus fumigatus in cystic fibrosis lungs http://ow.ly/XXwv30furqs
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- 2017
8. Laser-Assisted Intradermal Delivery of Adjuvant-Free Vaccines Targeting XCR1+ Dendritic Cells Induces Potent Antitumoral Responses
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Bjarne Bogen, Dorothea Terhorst, Reto Crameri, Sandrine Henri, Elmira Lechat, Camille Malosse, Reinhard Braun, Samira Tamoutounour, Bernard Malissen, Anna Baranska, Even Fossum, and Mattia Garbani
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Chemokine ,XCR1 ,Injections, Intradermal ,Ovalbumin ,medicine.medical_treatment ,T cell ,Immunology ,Melanoma, Experimental ,Cancer Vaccines ,Mice ,T-Lymphocyte Subsets ,Neoplasms ,medicine ,Animals ,Immunology and Allergy ,Mice, Knockout ,biology ,business.industry ,Melanoma ,Dendritic Cells ,Immunotherapy ,medicine.disease ,Chemokines, C ,Tumor Burden ,Adaptor Proteins, Vesicular Transport ,Disease Models, Animal ,medicine.anatomical_structure ,Myeloid Differentiation Factor 88 ,biology.protein ,Receptors, Chemokine ,business ,Adjuvant ,CD8 ,Protein Binding ,XCL1 - Abstract
The development of vaccines inducing efficient CD8+ T cell responses is the focus of intense research. Dendritic cells (DCs) expressing the XCR1 chemokine receptor, also known as CD103+ or CD8α+ DCs, excel in the presentation of extracellular Ags to CD8+ T cells. Because of its high numbers of DCs, including XCR1+ DCs, the skin dermis is an attractive site for vaccine administration. By creating laser-generated micropores through the epidermis, we targeted a model protein Ag fused to XCL1, the ligand of XCR1, to dermal XCR1+ DCs and induced Ag-specific CD8+ and CD4+ T cell responses. Efficient immunization required the emigration of XCR1+ dermal DCs to draining lymph nodes and occurred irrespective of TLR signaling. Moreover, a single intradermal immunization protected mice against melanoma tumor growth in prophylactic and therapeutic settings, in the absence of exogenous adjuvant. The mild inflammatory milieu created in the dermis by skin laser microporation itself most likely favored the development of potent T cell responses in the absence of exogenous adjuvants. The existence of functionally equivalent XCR1+ dermal DCs in humans should permit the translation of laser-assisted intradermal delivery of a tumor-specific vaccine targeting XCR1+ DCs to human cancer immunotherapy. Moreover, considering that the use of adjuvants in vaccines is often associated with safety issues, the possibility of inducing protective responses against melanoma tumor growth independently of the administration of exogenous adjuvants should facilitate the development of safer vaccines.
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- 2015
9. Artificial human sera: a breakthrough?
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Reto Crameri, University of Zurich, and Crameri, R
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0301 basic medicine ,2403 Immunology ,business.industry ,Immunology ,610 Medicine & health ,Computational biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Text mining ,030228 respiratory system ,10183 Swiss Institute of Allergy and Asthma Research ,2723 Immunology and Allergy ,Immunology and Allergy ,Medicine ,business - Published
- 2016
10. EAACI Molecular Allergology User's Guide
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Jörg Kleine-Tebbe, Sandip D. Kamath, Karin Hoffmann-Sommergruber, Simon Blank, Kirsten Beyer, M. Ebisawa, Daniela Posa, Thomas A.E. Platts-Mills, Joaquín Sastre, Mika J. Mäkelä, Elide A. Pastorello, Philippe Eigenmann, Nikolaos G. Papadopoulos, Peter Schmid-Grendelmeier, Richard W. Weber, Fatima Ferreira, Luis Caraballo, Helen A. Brough, Edward F. Knol, J-C Caubet, Andreas L. Lopata, Annette Kuehn, Ioana Agache, Tilo Biedermann, Martine Morisset, Stefan Vieths, Antonella Muraro, Barbara Bohle, Johannes Schmid, Barbara Ballmer-Weber, Verena Niederberger, Stephanie Hofmaier, R. van Ree, Peter Korošec, Heimo Breiteneder, Magnus Wickman, Gideon Lack, Thilo Jakob, Martin Glatz, Rudolf Valenta, Lars K. Poulsen, Philipp P. Bosshard, Anna Nowak-Wegrzyn, Gabrielle Pauli, Rob C. Aalberse, Thomas Hawranek, Marek Jutel, Gabriele Gadermaier, Hans Jürgen Hoffmann, Uta Jappe, Nikolaos Douladiris, M. van Hage, Reto Crameri, P W Hellings, Enrico Scala, Janet M. Davies, Riccardo Asero, Domingo Barber, Monika Raulf, Markus Ollert, Christiane Hilger, Robert G. Hamilton, M. B. Bilò, Montserrat Fernandez-Rivas, Paolo Maria Matricardi, Landsteiner Laboratory, Ear, Nose and Throat, AII - Amsterdam institute for Infection and Immunity, APH - Amsterdam Public Health, Experimental Immunology, Caubet, Jean-Christoph Roger J-P, and Eigenmann, Philippe
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0301 basic medicine ,Hypersensitivity, Immediate ,Allergy ,component-resolved diagnosis ,diagnosis ,Basophil ,medicine.disease_cause ,Immunoglobulin E ,Pediatrics ,profilins ,0302 clinical medicine ,Allergen ,Medicine ,Immunology and Allergy ,guidelines ,parvalbumins ,ddc:618 ,panallergens ,polcalcins ,biology ,atopic dermatitis ,food and beverages ,Perinatology ,3. Good health ,and Child Health ,medicine.anatomical_structure ,IgE ,lipocalins ,microarray ,Anaphylaxis ,Dander ,molecular allergology ,precision medicine ,Immunology ,Immunologic Tests ,tropomyosins ,03 medical and health sciences ,Food allergy ,diagnostic algorithms ,anaphylaxis ,Journal Article ,Humans ,Pediatrics, Perinatology, and Child Health ,House dust mite ,food allergy ,business.industry ,Allergens ,asthma ,biology.organism_classification ,medicine.disease ,allergy ,pathogenesis-related protein family 10 ,allergy diagnosis ,030104 developmental biology ,030228 respiratory system ,IgE cross-reactivity ,Pediatrics, Perinatology and Child Health ,biology.protein ,non-specific lipid transfer proteins ,serum albumins ,business ,Biomarkers - Abstract
The availability of allergen molecules ('components') from several protein families has advanced our understanding of immunoglobulin E (IgE)-mediated responses and enabled 'component-resolved diagnosis' (CRD). The European Academy of Allergy and Clinical Immunology (EAACI) Molecular Allergology User's Guide (MAUG) provides comprehensive information on important allergens and describes the diagnostic options using CRD. Part A of the EAACI MAUG introduces allergen molecules, families, composition of extracts, databases, and diagnostic IgE, skin, and basophil tests. Singleplex and multiplex IgE assays with components improve both sensitivity for low-abundance allergens and analytical specificity; IgE to individual allergens can yield information on clinical risks and distinguish cross-reactivity from true primary sensitization. Part B discusses the clinical and molecular aspects of IgE-mediated allergies to foods (including nuts, seeds, legumes, fruits, vegetables, cereal grains, milk, egg, meat, fish, and shellfish), inhalants (pollen, mold spores, mites, and animal dander), and Hymenoptera venom. Diagnostic algorithms and short case histories provide useful information for the clinical workup of allergic individuals targeted for CRD. Part C covers protein families containing ubiquitous, highly cross-reactive panallergens from plant (lipid transfer proteins, polcalcins, PR-10, profilins) and animal sources (lipocalins, parvalbumins, serum albumins, tropomyosins) and explains their diagnostic and clinical utility. Part D lists 100 important allergen molecules. In conclusion, IgE-mediated reactions and allergic diseases, including allergic rhinoconjunctivitis, asthma, food reactions, and insect sting reactions, are discussed from a novel molecular perspective. The EAACI MAUG documents the rapid progression of molecular allergology from basic research to its integration into clinical practice, a quantum leap in the management of allergic patients.
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- 2016
11. Interleukins (from IL-1 to IL-38), interferons, transforming growth factor b, and TNF-a : Receptors, functions, and roles in diseases
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Anna Głobińska, Cezmi A. Akdis, Terufumi Kubo, Paulina Wawrzyniak, Can Altunbulakli, Judith Olzhausen, Reto Crameri, Arturo Rinaldi, Andrzej Eljaszewicz, Zsolt István Komlósi, Umut Can Kucuksezer, Angela Treis, Barbara Stanic, Marcin Wawrzyniak, Thomas Eiwegger, Moira Prati, Claudio Rhyner, Ana Rebane, Marija Pezer, Milena Sokolowska, Oliver F. Wirz, Remo Frei, Mübeccel Akdis, Alar Aab, Su Duan, Kursat A Azkur, Liam O'Mahony, Carly Huitema, Ruth Ferstl, Kerstin Wanke, Patricia Konieczna, Kazunari Sugita, Rita Costa, Lena Hess, Josefina Zakzuk, Hideaki Morita, Nóra Kovács, Mattia Garbani, Norbert Meyer, and Willem van de Veen
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0301 basic medicine ,Immunology ,Biology ,T-Lymphocytes, Regulatory ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Transforming Growth Factor beta ,Immunology and Allergy ,Animals ,Humans ,Receptor ,Toll-like receptor ,Innate immune system ,Tumor Necrosis Factor-alpha ,Interleukins ,Innate lymphoid cell ,FOXP3 ,Dendritic cell ,Acquired immune system ,3. Good health ,030104 developmental biology ,Immune System Diseases ,Interferons ,030215 immunology ,Cytokines ,interleukins ,T cells ,B cells ,dendritic cells ,innate immune response ,adaptive immune response ,humoral immune response ,allergy and asthma - Abstract
There have been extensive developments on cellular and molecular mechanisms of immune regulation in allergy, asthma, autoimmune diseases, tumor development, organ transplantation, and chronic infections during the last few years. Better understanding the functions, reciprocal regulation, and counterbalance of subsets of immune and inflammatory cells that interact through interleukins, interferons, TNF-α, and TGF- β offer opportunities for immune interventions and novel treatment modalities in the era of development of biological immune response modifiers particularly targeting these molecules or their receptors. More than 60 cytokines have been designated as interleukins since the initial discoveries of monocyte and lymphocyte interleukins (called IL-1 and IL-2, respectively). Studies of transgenic or gene- deficient mice with altered expression of these cytokines or their receptors and analyses of mutations and polymorphisms in human genes that encode these products have provided essential information about their functions. Here we review recent developments on IL-1 to IL-38, TNF-α, TGF-β, and interferons. We highlight recent advances during the last few years in this area and extensively discuss their cellular sources, targets, receptors, signaling pathways, and roles in immune regulation in patients with allergy and asthma and other inflammatory diseases.
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- 2016
12. IgE sensitization profiles differ between adult patients with severe and moderate atopic dermatitis
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Annika Scheynius, Lena Lundeberg, Reto Crameri, Gustav Wikberg, Rudolf Valenta, Catharina Johansson, Christian Lupinek, Irene Mittermann, and University of Zurich
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0301 basic medicine ,Male ,Allergy ,Staphylococcus ,Eczema ,Atopic Dermatitis ,Plant Science ,medicine.disease_cause ,Immunoglobulin E ,Pathology and Laboratory Medicine ,Allergen ,10183 Swiss Institute of Allergy and Asthma Research ,Allergies ,Medicine and Health Sciences ,Staphylococcus Aureus ,Sensitization ,Mites ,Multidisciplinary ,biology ,Allergic Diseases ,Plant Anatomy ,Atopic dermatitis ,Plants ,Middle Aged ,Recombinant Proteins ,3. Good health ,Bacterial Pathogens ,medicine.anatomical_structure ,Medical Microbiology ,Malassezia sympodialis ,Medicine ,Pollen ,Female ,Malassezia ,Pathogens ,Research Article ,Adult ,Arthropoda ,Adolescent ,Science ,Immunology ,Immunoblotting ,Molecular Probe Techniques ,610 Medicine & health ,Dermatology ,1100 General Agricultural and Biological Sciences ,Research and Analysis Methods ,Microbiology ,Dermatitis, Atopic ,03 medical and health sciences ,Young Adult ,1300 General Biochemistry, Genetics and Molecular Biology ,medicine ,otorhinolaryngologic diseases ,Animals ,Humans ,Grasses ,Molecular Biology Techniques ,Microbial Pathogens ,Molecular Biology ,Aged ,Skin Tests ,House dust mite ,1000 Multidisciplinary ,Bacteria ,Organisms ,Biology and Life Sciences ,Allergens ,biology.organism_classification ,medicine.disease ,Invertebrates ,030104 developmental biology ,Case-Control Studies ,biology.protein ,Cats ,Clinical Immunology ,Clinical Medicine ,Biomarkers - Abstract
BackgroundAtopic dermatitis (AD) is a complex chronic inflammatory disease where allergens can act as specific triggering factors.AimTo characterize the specificities of IgE-reactivity in patients with AD to a broad panel of exogenous allergens including microbial and human antigens.MethodologyAdult patients with AD were grouped according to the SCORAD index, into severe (n = 53) and moderate AD (n = 126). As controls 43 patients were included with seborrhoeic eczema and 97 individuals without history of allergy or skin diseases. Specific IgE reactivity was assessed in plasma using Phadiatop®, ImmunoCap™, micro-arrayed allergens, dot-blotted recombinant Malassezia sympodialis allergens, and immune-blotted microbial and human proteins.ResultsIgE reactivity was detected in 92% of patients with severe and 83% of patients with moderate AD. Sensitization to cat allergens occurred most frequently, followed by sensitization to birch pollen, grass pollen, and to the skin commensal yeast M. sympodialis. Patients with severe AD showed a significantly higher frequency of IgE reactivity to allergens like cat (rFel d 1) and house dust mite (rDer p 4 and 10), to Staphylococcus aureus, M. sympodialis, and to human antigens. In contrast, there were no significant differences in the frequencies of IgE reactivity to the grass pollen allergens rPhl p 1, 2, 5b, and 6 between the two AD groups. Furthermore the IgE reactivity profile of patients with severe AD was more spread towards several different allergen molecules as compared to patients with moderate AD.ConclusionWe have revealed a hitherto unknown difference regarding the molecular sensitization profile in patients with severe and moderate AD. Molecular profiling towards allergen components may provide a basis for future investigations aiming to explore the environmental, genetic and epigenetic factors which could be responsible for the different appearance and severity of disease phenotypes in AD.
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- 2016
13. Differential cytokine induction by the human skin-associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control subjects
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Lennart M. Roesner, Annika Scheynius, Reto Crameri, Mattia Garbani, Thomas Werfel, Susanne Hradetzky, Annice Heratizadeh, and University of Zurich
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medicine.medical_treatment ,Immunology ,Human skin ,610 Medicine & health ,Autoantigens ,Dermatitis, Atopic ,03 medical and health sciences ,0302 clinical medicine ,Thioredoxins ,10183 Swiss Institute of Allergy and Asthma Research ,Healthy control ,medicine ,Immunology and Allergy ,Humans ,030304 developmental biology ,0303 health sciences ,2403 Immunology ,business.industry ,Atopic dermatitis ,Allergens ,medicine.disease ,Cytokine ,Case-Control Studies ,2723 Immunology and Allergy ,Cytokines ,Thioredoxin ,business ,030215 immunology - Published
- 2015
14. Immune regulation by intralymphatic immunotherapy with modular allergen translocation MAT vaccine
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Nesrin Özören, Cemalettin Bekpen, Reto Crameri, Claudio Rhyner, A. Zaleska, William W. Kwok, Angela Treis, Oscar Palomares, D. Demiröz, Ozge Soyer, Mübeccel Akdis, W. van de Veen, H. Rose, Thomas M. Kündig, Marek Jutel, Thomas Eiwegger, Cezmi A. Akdis, Michael B. Soyka, Gabriela Senti, Stefan Söllner, University of Zurich, and Akdis, M
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T-Lymphocytes ,medicine.medical_treatment ,Blotting, Western ,Immunology ,Caspase 1 ,610 Medicine & health ,10045 Clinic for Otorhinolaryngology ,Real-Time Polymerase Chain Reaction ,medicine.disease_cause ,Peripheral blood mononuclear cell ,Immune tolerance ,Allergen ,Immune system ,10183 Swiss Institute of Allergy and Asthma Research ,Fel d 1 ,medicine ,Humans ,Immunology and Allergy ,Glycoproteins ,Vaccines ,2403 Immunology ,Microscopy, Confocal ,biology ,business.industry ,10177 Dermatology Clinic ,Inflammasome ,Immunotherapy ,Flow Cytometry ,3. Good health ,Desensitization, Immunologic ,biology.protein ,2723 Immunology and Allergy ,business ,medicine.drug - Abstract
Background Allergen specific immunotherapy (SIT) faces problems related to side effects and limited efficacy. Direct administration of allergen extracts into lymph nodes induces increased specific IgG production and T cell responses using significantly lower allergen doses. Methods In this study mechanisms of immune regulation by MAT vaccines in vitro and in allergen SIT of cat Allergic rhinitis patients who received 3 inguinal intra lymph node injections of MAT Fel d 1 vaccine were investigated in PBMC and cell cultures for specific T cell proliferation Fel d 1 tetramer specific responses and multiple immune regulatory molecules. Results MAT Fel d 1 vaccine was efficiently internalized by antigen presenting cells. This was followed by precaspase 1 cleavage to caspase 1 and secretion of IL 1ß indicating inflammasome activation. Mat Fel d 1 induced specific T cell proliferation and an IL 10 and IFN ? dominated T cell responses with decreased Th2 cytokines at 100 times lower doses than Fel d 1. Induction of immune tolerance by MAT Fel d 1 ILIT involved multiple mechanisms of immune suppression. Early Fel d 1 specific T cell activation was followed by full T cell unresponsiveness to allergen after 1 year in the MAT Fel d 1 group characterized by increased allergen specific T regulatory cells decreased circulating Fel d 1 tetramer positive cells increased IL 10 and FOXP3 expression and change in the HR2/HR1 ratio toward HR2. Conclusions This study demonstrates the induction of allergen tolerance after 3 intra lymph node injections of MAT Fel d 1 vaccine mediated by increased cellular internalization of the allergen activation of inflammasome and generation of allergen specific peripheral T cell tolerance. © 2014 John Wiley Sons A/S. Published by John Wiley Sons Ltd.
- Published
- 2014
15. ITOC2 – 016. Effective vaccination against melanoma in an animal study: Combination of laser-assisted dermal skin delivery and cross-presenting XCR1+ dermal DCs targeting
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Dora, Terhorst D.T., primary, Even, Fossum E.F., additional, Anna, Baranska A.B., additional, Samira, Tamoutounour S.T., additional, Camille, Malosse C.M., additional, Mattia, Garbani M.G., additional, Elmira, Lechat E.L., additional, Reto, Crameri R.C., additional, Roland, Winteler R.W., additional, Bjarne, Bogen B.B., additional, Bernard, Malissen B.M., additional, and Sandrine, Henri S.H., additional
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- 2015
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- View/download PDF
16. Abstract 2518: Effective vaccination against melanoma in an animal study: Combination of laser-assisted dermal skin delivery and cross-presenting XCR1+ dermal DCs targeting
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Sandrine Henri, Bernard Malissen, Anna Baranska, Mattia Garbani, Even Fossum, Camille Malosse, Elmira Lechat, Bjarne Bogen, Dorothea Terhorst, Reto Crameri, Samira Tamoutounour, and Roland Winteler
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Cancer Research ,integumentary system ,business.industry ,Melanoma ,T cell ,Cancer ,Dendritic cell ,medicine.disease ,medicine.anatomical_structure ,Oncology ,Antigen ,Immunology ,medicine ,Cytotoxic T cell ,business ,CD8 ,XCL1 - Abstract
Background/Aim/Method The induction of CD8+ cytotoxic T lymphocytes (CTL) is critical to eradicate tumor cells. In mouse skin, a subset of dendritic cell characterised by the expression of the chemokine receptor XCR1 is highly potent to activate CD8+ T cells. By combining PLEASE-assisted laser poration of the skin allowing dermal delivery and the use of dimeric vaccine molecule in which the XCR1 ligand, Xcl1, was fused to the antigen, we targeted specifically this XCR1+ dermal subset. Results Dermal delivery of this vaccine molecule after skin laser-poration induced specific strong T cell proliferation in an XCR1-dependent manner. A single immunization allowed protecting against melanoma in both therapeutic and prophylactic protocols. Discussion & Conclusion Thus, specific targeting of cross-presenting skin DCs represents a promising vaccine strategy for induction of CD8+ T cell responses and protection against cancers. Citation Format: Dorothea Terhorst, Even Fossum, Anna Baranska, Samira Tamoutounour, Camille Malosse, Mattia Garbani, Elmira Lechat, Reto Crameri, Roland Winteler, Bjarne Bogen, Bernard Malissen, Sandrine Henri. Effective vaccination against melanoma in an animal study: Combination of laser-assisted dermal skin delivery and cross-presenting XCR1+ dermal DCs targeting. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2518. doi:10.1158/1538-7445.AM2015-2518
- Published
- 2015
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