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6,138 results on '"Requimte"'

1. Bioavailability of Red Wine Anthocyanins

Author: REQUIMTE/LAQV, Departamento de Química e Bioquímica, FCUP, Center for Health Technology and Services Research, and Vinoflavo
Published: 2016

2. Soluble Mediators Potentially Involved in Pruritus Associated to Cutaneous T-Cell Lymphomas and Mastocytosis: A Cross-Sectional Study

Author: Abreu M, Lima M, Mirand M, Coimbra S, Santos-Silva A, and Ucibio Requimte, Porto, Portugal
Subjects: medicine.anatomical_structure, Cross-sectional study, business.industry, hemic and lymphatic diseases, T cell, Immunology, Medicine, skin and connective tissue diseases, business
Abstract: Pruritus is a major distressing symptom, common in inflammatory diseases, like Cutaneous T-Cell Lymphoma (CTCL) and mastocytosis. We aimed to study the involvement of some molecules, namely, cytokines, neuromediators, endothelial adhesion molecules and angiogenic factors, in the severity of pruritus associated to CTCL and mastocytosis. CTCL - Mycosis Fungoides (MF, n=17) and Sézary syndrome (SS, n=10) and mastocytosis patients (n=17) were evaluated. Interleukin (IL)-8, IL-31, Vascular Endothelial Growth Factor (VEGF), E-selectin, serotonin and C-reactive protein (CRP) levels, were assessed; tryptase was measured in mastocytosis. Pruritus severity was assessed, using a Visual Analogue Scale (VAS). Compared to controls (n=29), CTCL patients presented higher CRP and IL-31. SS patients had higher IL-31, E-selectin and CRP than MF patients and controls. Itch correlated with IL- 31 and E-selectin, when considering all CTCL patients; in SS, itch correlated with E-selectin. Advanced CTCL stages revealed higher IL-31, E-selectin and CRP than early stages, and controls; itch intensity correlated with IL-31 and E-selectin, in advanced stages. Mastocytosis showed higher serotonin and VEGF, compared to controls, and itch intensity correlated with tryptase. Data suggest that in mastocytosis, serotonin is an important biomarker and that tryptase levels reflect itch intensity; IL-31 and E-selectin appear to be more important mediators in CTCL and strongly correlated with itch severity. The different involvement of studied mediators, probably due to different immune responses, suggests that different mechanisms underlie these diseases and may lead to different itch mechanisms.
Published: 2021
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3. Nutritional, health status and well-being at work: gender’s differences

Author: Rede de Química e Tecnologia Laqv Requimte, J. Lima, Centro de Investigação em Produção Agroalimentar Sustentável GreenUPorto, Sofia Almeida Costa, and Ada Rocha
Subjects: Cultural Studies, Gerontology, History, Literature and Literary Theory, Work (electrical), Well-being, Psychology
Published: 2018
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4. Monitorização e notificação de efeitos adversos ao sistema de Farmacovigilância Europeu da nova terapêutica para a Hepatite C

Author: Costa, Cláudia, FP-ENAS (Unidade de Investigação UFP em Energia, Ambiente e Saúde), CEBIMED (Centro de Estudos em Biomedicina), Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Portugal., Marques, Joana, Centre for Research in Health Technologies and Information Systems (CINTESIS), Faculdade de Medicina da Universidade do Porto, Portugal, Capela, João Paulo, and UCIBIO/REQUIMTE (Rede de Química e Tecnologia), Laboratório de Toxicologia, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Portugal.
Subjects: Hepatite C, vírus da Hepatite C, Harvoni®, efeitos adversos, notificação, farmacovigilância
Published: 2017

5. Fe3O4-Au core-shell nanoparticles as a multimodal platform for in vivo imaging and focused photothermal therapy

Author: Ana M. Beltrán, Alejandro Domínguez, Manuel Leal, Carlos Caro, John R. Pearson, María L. García-Martín, Yilian Fernández-Afonso, Pedro Quaresma, Jesús M. de la Fuente, Ricardo Franco, Jose María Páez-Muñoz, Eulália Pereira, Francisco Gámez, UCIBIO - Applied Molecular Biosciences Unit, DQ - Departamento de Química, Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica, Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. FQM102: Estereoquímica y Síntesis Asimétrica, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, [Caro,C, Páez-Muñoz,JM, Domínguez,A, Pearson,JR, García-Martín,ML] BIONAND—Centro Andaluz de Nanomedicina y Biotecnología (Junta de Andalucía-Universidad de Málaga), Málaga, Spain. [Gámez,F] Departamento de Química Física, Universidad de Granada, Granada, Spain. [Quaresma,P: Pereira,E] REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Porto, Portugal. [Pernía Leal,M] Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain. [Beltrán,AM] Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, Sevilla, Spain. [Fernandez-Afonso,Y, De la Fuente,JM] Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, Zaragoza, Spain. [De la Fuente,JM, García-Martín,ML] Biomedical Research Networking Center in Bioengineering, Biomaterials &Nanomedicine (CIBER-BBN), Madrid, Spain [Franco,R] UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal., Financial support was provided by the Spanish Ministry of Economy, Industry and Competitiveness (CTQ2017-86655-R and BIO2017-84246-C2-1-R), Fondo Social de la DGA (grupos DGA) and by the Regional Ministry of Health of Andalusia (OH-0026-2018). This work was supported by the Associate Laboratory for Green Chemistry—LAQV, and by the Applied Molecular Biosciences Unit –UCIBIO, both financed by Portuguese national funds from FCT/MCTES (UIDB/04378/2020 and UIDB/50006/2020)., Ministerio de Economía, Industria y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Diputación General de Aragón, Junta de Andalucía, Fundação para a Ciência e a Tecnologia (Portugal), and Ministério da Ciência, Tecnologia e Ensino Superior (Portugal)
Subjects: Imagen por resonancia magnética, Phenomena and Processes::Metabolic Phenomena::Pharmacokinetics::Tissue Distribution [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Magnetic Resonance Imaging [Medical Subject Headings], Nanoparticle, Pharmaceutical Science, lcsh:RS1-441, multimodal imaging, 02 engineering and technology, TomografíacComputarizada por rayos X, 01 natural sciences, Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Atmosphere::Weather::Temperature::Hot Temperature [Medical Subject Headings], chemistry.chemical_compound, Organisms::Eukaryota::Animals [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Multimodal Imaging [Medical Subject Headings], Zebrafish, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Fishes::Cypriniformes::Cyprinidae::Zebrafish [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Surface Plasmon Resonance [Medical Subject Headings], Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles::Metal Nanoparticles [Medical Subject Headings], Temperature, Nanopartículas, Imaging-guided therapy, Temperatura, Diseases::Neoplasms [Medical Subject Headings], contrast agent, 021001 nanoscience & nanotechnology, Photothermal therapy, Imagen multimodal, Neoplasias, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia [Medical Subject Headings], imaging-guided therapy, Tissues, Contrast agent, 0210 nano-technology, Preclinical imaging, Iron oxide nanoparticles, MRI, CT, Terapia fototérmica, Biodistribution, Biocompatibility, Medios de contraste, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Tomography::Tomography, X-Ray::Tomography, X-Ray Computed [Medical Subject Headings], Pez cebra, Technology and Food and Beverages::Technology, Industry, and Agriculture::Manufactured Materials::Biomedical and Dental Materials::Polymers::Plastics::Polyvinyls::Povidone [Medical Subject Headings], 010402 general chemistry, Article, lcsh:Pharmacy and materia medica, Therapeutic index, In vivo, Multimodal imaging, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings], Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Diagnostic Uses of Chemicals::Contrast Media [Medical Subject Headings], 0104 chemical sciences, Tejidos, chemistry, Nanoparticles, Biomedical engineering
Abstract: In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable “light-to-heat” conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model., Spanish Ministry of Economy, Industry and Competitiveness (CTQ2017-86655-R and BIO2017-84246-C2-1-R), Fondo Social de la DGA (grupos DGA) and by the Regional Ministry of Health of Andalusia (OH-0026-2018)., Associate Laboratory for Green Chemistry—LAQV, Applied Molecular Biosciences Unit –UCIBIO, Portuguese national funds from FCT/MCTES (UIDB/04378/2020 and UIDB/50006/2020)
Published: 2021

6. Natural Variation of Hazelnut Allergenicity: Is There Any Potential for Selecting Hypoallergenic Varieties?

Author: Gilberto Igrejas, Sandra Cabo, Ana Paula Silva, Berta Gonçalves, Mélanie Hillion, Michel Hébraud, Joana Costa, Isabel Mafra, Miguel Ribeiro, Universidade de Trás-os-Montes e Alto Douro (UTAD), Universidade do Trás-os-Montes e Alto Douro, LAQV-REQUIMTE, Universidade NOVA de Lisboa, Departamento de Quimica (REQUIMTE), Universidade de Lisboa (ULISBOA)-Centro de Quimica Fina e Biotecnologia, Centro de Investigação e de Tecnologias Agro-Ambientais e Biológicas (CITAB), University of Trás-os-Montes and Alto Douro [Portugal] (UTAD), INRAE, Plateforme d’Exploration du Métabolisme, Composante Protéomique (PFEMcp), 63122 Saint-Genès Champanelle, Microbiologie Environnement Digestif Santé (MEDIS), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Associate Laboratory for Green Chemistry-LAQV - Fundacao para a Ciencia e Tecnologia (FCT) UIDB 50006/2020national project AlleRiskAssess PTDC/BAA-AGR/31720/2017NORTE-01-0145-FEDER-00001national project HazelOmics (LAQV-REQUIMTE) INTERACT project-'Integrative Research in Environment, Agro-Chains and Technology' NORTE-01-0145-FEDER-000017European Union (EU), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Universidade de Lisboa = University of Lisbon (ULISBOA)-Centro de Quimica Fina e Biotecnologia, Plateforme Exploration du Métabolisme (PFEM), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-MetaboHUB-Clermont, and MetaboHUB-MetaboHUB
Subjects: 0301 basic medicine, Male, Proteomics, medicine.medical_treatment, 0302 clinical medicine, immunoblot, Exclusion diet, Food science, Desensitization (medicine), Plant Proteins, 2. Zero hunger, Nutrition and Dietetics, genetic diversity, respiratory system, Middle Aged, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, nutrition, Hazelnut allergy, Child, Preschool, proteogenomics, Female, Nut Hypersensitivity, lcsh:Nutrition. Foods and food supply, Food Hypersensitivity, Adult, Adolescent, lcsh:TX341-641, Biology, Natural variation, hazelnut-allergic patients’ sera, Article, 03 medical and health sciences, Young Adult, Corylus, Food allergy, medicine, Humans, hazelnut allergy, Aged, Genetic diversity, Genetic Variation, Hypoallergenic, Allergens, Antigens, Plant, Immunoglobulin E, medicine.disease, hazelnut-allergic patients' sera, respiratory tract diseases, 030104 developmental biology, Human nutrition, 030228 respiratory system, Food Science
Abstract: International audience; Hazelnuts (Corylus avellanaL.) have an important role in human nutrition and health. However, they are a common cause of food allergy. Due to hazelnut varietal diversity, variety-dependent differences in the IgE-binding properties may be suspected, which could allow therapeutic strategies based on the use of hypoallergenic varieties to induce desensitization. In a proteogenomic approach, we aimed to evaluate the allergenic potential of a genetically diverse set of hazelnuts (n = 13 varieties). Minor differences were found at the level of genes encoding important allergens, namely Cor a 8, Cor a 9, and Cor a 14. Nevertheless, IgE-reactivity was similar for all varieties using sera from seven allergic individuals. The predominant IgE-reactive proteins were Cor a 9 (100%) and Cor a 1.04 (60%), with the former being the most frequently identified by a two-dimensional gel electrophoresis (2-DE)-based proteomic approach. Therefore, it seems that the conventional exclusion diet will hold its ground for the time being.
Published: 2020
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7. Inhibition Mechanisms of Wine Polysaccharides on Salivary Protein Precipitation

Author: Nuno Mateus, Thierry Doco, Ignacio García-Estévez, Mafalda Santos Silva, Victor de Freitas, Elsa Brandão, Susana Soares, Pascale Williams, REQUIMTE/LAQV Departamento de Quimica e Bioquimica, Faculdade de Ciencias, Universidade do Porto, Sciences Pour l'Oenologie (SPO), Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), REQUIMTE/LAQV, Faculdade de Ciências, FCT/MEC (PTDC/AGR-TEC/6547/2014, NORTE-01-0145-FEDER-000011), European Union (EU), FEDER-Interreg Espana-Portugal Programme 0377_IBERPHENOL_6_E, Portuguese Foundation for Science and Technology UID/QUI/50006/2019, and IBERPHENOL project
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV]Life Sciences [q-bio], salivary proteins, Peptide, astringency, Polysaccharide, Galactans, 01 natural sciences, chemistry.chemical_compound, 0404 agricultural biotechnology, Polysaccharides, Arabinogalactan, procyanidin B2, tannins, Chemical Precipitation, Humans, Tannin, Salivary Proteins and Peptides, Sodium dodecyl sulfate, wine, Saliva, Polyacrylamide gel electrophoresis, Ternary complex, Procyanidin B2, chemistry.chemical_classification, rhamnogalacturonan II, 010401 analytical chemistry, 04 agricultural and veterinary sciences, General Chemistry, 040401 food science, 0104 chemical sciences, chemistry, Biochemistry, punicalagin, Pectins, arabinogalactan proteins, General Agricultural and Biological Sciences, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: In this work, high-performance liquid chromatography, fluorescence quenching, nephelometry, and sodium dodecyl sulfate polyacrylamide gel electrophoresis were used to study the effect of polysaccharides naturally present in wine [rhamnogalacturonan II (RG II) and arabinogalactan proteins (AGPs)] on the interaction between salivary proteins (SP) together present in saliva and tannins (punicalagin (PNG) and procyanidin B2). In general, the RG II fraction was more efficient to inhibit SP precipitation by tannins, especially for acidic proline-rich proteins (aPRPs) and statherin/P-B peptide, than AGPs. The RG II fraction can act mainly by a competition mechanism in which polysaccharides compete by tannin binding. However, in the presence of Na+ ions in solution, no RG II effect was observed on SP-tannin interactions. On the other hand, dependent upon the saliva sample as well as the tannin studied, AGPs can act by both mechanisms, competition and ternary (formation of a ternary complex with SP-tannin aggregates enhancing their solubility).
Published: 2020
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8. Next-generation care pathways for allergic rhinitis and asthma multimorbidity: A model for multimorbid non-communicable diseases—Meeting Report (Part 2)

Author: Josep M. Antó, Nick A. Guldemond, Ulysse Rodts, Bolesław Samoliński, Olga Lourenço, Mohamed H. Shamji, Tomohisa Iinuma, Rianne van der Kleij, Alkis Togias, W. J. Fokkens, Gert Marien, Derek K. Chu, Mario Sánchez-Borges, Daniel Laune, Desiree Larenas-Linneman, Anna Bedbrook, Guy Brusselle, Joaquim Mullol, Lars Münter, Ludger Klimek, Sanna Toppila-Salmi, Eugene Cash, Christine Rolland, Ioanna Tsiligianni, Isabelle Bosse, Dana Wallace, Motohiro Ebisawa, Abigail Phillips, Torsten Zuberbier, G. Walter Canonica, Elísio Costa, Samuel Benveniste, Yoshitaka Okamoto, Holger J. Schünemann, Moises A. Calderon, Sinthia Bosnic-Anticevich, Mike Bewick, Elaine Colgan, Arunas Valiulis, Sian Williams, Samantha Walker, Ignacio J. Ansotegui, David Somekh, Isabella Annesi-Maesano, Victoria Cardona, Wienczyslawa Czarlewski, Claus Bachert, Ana Maria Carriazo, João Fonseca, Nils Billo, Violeta Kvedariene, Ioana Agache, Maria Teresa Ventura, Jim Phillips, Piotr Kuna, Hilary Pinnock, Lorenzo Cecchi, F. Portejoie, Jean-Luc Fauquert, Alvaro A. Cruz, Jean Bousquet, Nikos Papadopoulos, Omar S. Usmani, Gabrielle L. Onorato, Arzu Yorgancioglu, Pablo Quinones-Delgado, Oliver Pfaar, Luis Caraballo, Stephen R. Durham, L. T. T. Le, João O. Malva, Maritta Perala, Enrica Menditto, Nhân Pham-Thi, Marina Erhola, Maddalena Illario, Ear, Nose and Throat, AII - Inflammatory diseases, University Hospital Montpellier, Montpellier, France, MACVIA-France, Fondation partenariale FMC VIA-LR, Montpellier, France, INSERM U1168, VIMA, Ageing and Chronic Diseases Epidemiological and Public Health Approaches, Villejuif, France, Université Versailles St-Quentin-en Yvelines, UMR-S 1168, Montigny le Bretonneux, France, Euforea, Brussels, Belgium, Charité, Universitätsmedizin Berlin, Humboldt-Universitätzu Berlin, Berlin, Germany, Berlin Institute of Health, Comprehensive Allergy Center, Department of Dermatology and Allergy, Berlin, Germany, EUFOREA, Brussels, Belgium, Allergy Department, Pasteur Institute, Paris, France, Faculty of Medicine, Transylvania University, Brasov, Romania, Epidemiology of Allergic and Respiratory Diseases, Department Institute Pierre Louis of Epidemiology and Public Health, INSERM and Sorbonne Universités, Medical School Saint Antoine, Paris, France, Department of Allergy and Immunology, Hospital Quirónsalud Bizkaia, Erandio, Spain, ISGlobAL, Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, CIBER Epidemiología y Salud Pública (CIBERESP), Barcelona, Spain, Upper Airways Research Laboratory, ENT Department, Ghent University Hospital, Ghent, Belgium, National Center of Expertise in Cognitive Stimulation (CEN STIMCO), Broca Hospital, Paris, France, Mines ParisTech CRI - PSL Research University, Fontainebleau, France, iQ4U Consultants Ltd, London, United Kingdom, Independent Consultant, Joensuu, Finland, Woolcock Institute of Medical Research, University of Sydney, Woolcock Emphysema Centre, Sydney Local Health District, Glebe, NSW, Australia, Allergist, La Rochelle, France, Department of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium, Imperial College London-National Heart and Lung Institute, London, United Kingdom, Personalized Medicine Clinic Asthma and Allergy, Humanitas Clinical and Research Center IRCCS, Rozzano (MI), Italy, Department of Biomedical Sciences, Humanitas University, Pieve Emanuele (MI), Italy, Institute for Immunological Research, University of Cartagena, Campus de Zaragocilla, Edificio Biblioteca Primer piso, Cartagena, Colombia, Foundation for the Development of Medical and Biological Sciences, Cartagena, Colombia, Allergy Section, Department of Internal Medicine, Hospital Vall d’Hebron and ARADyAL research network, Barcelona, Spain, Regional Ministry of Health of Andalusia, Seville, Spain, College of Psychology, Nova Southeastern University, School-related Psychological Assessments and Clinical Interventions Clinic, Ft Lauderdale, FL, United States, SOS Allergology and Clinical Immunology, USL Toscana Centro, Prato, Italy, Department of Health Research Methods, Evidence, and Impact, Division of Immunology and Allergy, Department of Medicine, McMaster University, Hamilton, ON, Canada, Department of Health, Social Services and Public Safety, Northern Ireland, Belfast, United Kingdom, UCIBIO, REQUIMTE, Faculty of Pharmacy, and Competence Center on Active and Healthy Ageing of University of Porto (AgeUPNetWork), University of Porto, Porto, Portugal, ProAR-Nucleo de Excelencia em Asma, Federal University of Bahia, Brasil and WHO GARD Executive Committee, Bahia, Brazil, Medical Consulting Czarlewski, Levallois, France, Allergy and Clinical Immunology Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom, Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Japan, National Institute for Health and Welfare, Helsinki, Finland, CHU Clermont-Ferrand, Unité d’Allergologie de l’Enfant, Pôle Pédiatrique, Hôpital Estaing, Clermont-Ferrand, France, Department of Otorhinolaryngology, Amsterdam University Medical Centres, AMC, Amsterdam, Netherlands, CINTESIS, Center for Research in Health Technology and Information Systems, Faculdade de Medicina da Universidade do Porto, Medida, Lda Porto, Portugal, Institute of Health Policy and Management iBMG, Erasmus University, Rotterdam, Netherlands, Department of Otorhinolaryngology, Chiba University Hospital, Chiba, Japan, Division for Health Innovation, Campania Region and Federico II University Hospital Naples (R and D and DISMET), Naples, Italy, Center for Rhinology and Allergology, Wiesbaden, Germany, Division of Internal Medicine, Asthma and Allergy, Barlicki University Hospital, Medical University of Lodz, Lodz, Poland, Institute of Biomedical Sciences, Department of Pathology, Faculty of Medicine, Vilnius University, Institute of Clinical Medicine, Clinic of Chest diseases and Allergology, Faculty of Medicine, Vilnius, Lithuania, Center of Excellence in Asthma and Allergy, Médica Sur Clinical Foundation and Hospital, México City, Mexico, KYomed INNOV, Montpellier, France, University of Medicine and Pharmacy, Hochiminh City, Viet Nam, Faculty of Health Sciences and CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal, Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Ageing@Coimbra EIP-AHA Reference Site, Coimbra, Portugal, CIRFF, Center of Pharmacoeconomics, University of Naples Federico II, Naples, Italy, Rhinology Unit and Smell Clinic, ENT Department, Hospital Clínic, Clinical and Experimental Respiratory Immunoallergy, IDIBAPS, CIBERES, University of Barcelona, Barcelona, Spain, Danish Committee for Health Education, Copenhagen East, Denmark, Division of Infection, Immunity and Respiratory Medicine, Royal Manchester Children’s Hospital, University of Manchester, Manchester, United Kingdom, Allergy Department, 2nd Pediatric Clinic, Athens General Children’s Hospital 'P and A Kyriakou', University of Athens, Athens, Greece, University of Oulu, Faculty of Medicine, Oulun Yliopisto, Finland, Department of Otorhinolaryngology, Head and Neck Surgery, Section of Rhinology and Allergy, University Hospital Marburg, Phillipps-Universität Marburg, Germany, Department of Health and Social Services, Welsh Government, Cardiff, United Kingdom, Centre For Empowering Patients and Communities, Dublin, Ireland, Asthma UK Centre for Applied Research, Usher Institute of Population Health Sciences and Informatics, The, University of Edinburgh, Edinburgh, United Kingdom, Agency for Social Services and Dependency, Regional Government for Equality, Social Policies and Conciliation of Andalucia, Seville, Spain, Association Asthme et Allergie, Paris, France, KanopyMed, Paris, France, Department of Prevention of Environmental Hazards and Allergology, Medical University of Warsaw, Warsaw, Poland, Allergy and Clinical Immunology Department, Centro Medico-Docente La Trinidad, Caracas, Venezuela, Immunomodulation and Tolerance Group, Imperial College London, and, Allergy and Clinical Immunology, Imperial College London, London, United Kingdom, European Health Futures Forum (EHFF), Dromahair, Ireland, Division of Allergy, Immunology, and Transplantation (DAIT), National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, United States, Skin and Allergy Hospital, Helsinki University Hospital, University of Helsinki, Helsinki, Finland, Health Planning Unit, Department of Social Medicine, Faculty of Medicine, University of Crete, Crete, Greece, International Primary Care Respiratory Group IPCRG, Aberdeen, United Kingdom, National Heart and Lung Institute (NHLI), Imperial College London, Royal Brompton Hospital, Airways Disease Section, London, United Kingdom, Asthma UK, London, United Kingdom, Nova Southeastern University, Fort Lauderdale, FL, United States, Vilnius University Faculty of Medicine, Institute of Clinical Medicine, Institute of Health Sciences, Vilnius, Lithuania, Department of Public Health and Primary Care, Leiden University Medical Center, Leiden, Netherlands, University of Bari Medical School, Unit of Geriatric Immunoallergology, Bari, Italy, Department of Pulmonary Diseases, Celal Bayar University, Faculty of Medicine, Manisa, Turkey, Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Uniersität zu Berlin, Berlin Institute of Health, Comprehensive Allergy-Centre, Department of Dermatology and Allergy, Berlin, Germany, CHU Arnaud de Villeneuve, 371 Avenue du Doyen Gaston Giraud, Montpellier Cedex 5, 34295, France, [Bousquet, Jean] Univ Hosp Montpellier, Montpellier, France, [Bousquet, Jean] Fdn Partenariale FMC VIA LR, MACVIA France, Montpellier, France, [Bedbrook, Anna] Fdn Partenariale FMC VIA LR, MACVIA France, Montpellier, France, [Onorato, Gabrielle L.] Fdn Partenariale FMC VIA LR, MACVIA France, Montpellier, France, [Portejoie, Fabienne] Fdn Partenariale FMC VIA LR, MACVIA France, Montpellier, France, [Bousquet, Jean] INSERM, Ageing & Chron Dis Epidemiol & Publ Hlth Approach, VIMA, U1168, Villejuif, France, [Bousquet, Jean] Univ Versailles St Quentin En Yvelines, UMR S 1168, Montigny Le Bretonneux, France, [Bousquet, Jean] Euforea, Brussels, Belgium, [Fokkens, Wytske J.] Euforea, Brussels, Belgium, [Marien, Gert] Euforea, Brussels, Belgium, [Bousquet, Jean] Humboldt Univ, Univ Med Berlin, Charite, Berlin, Germany, [Bousquet, Jean] Berlin Inst Hlth, Comprehens Allergy Ctr, Dept Dermatol & Allergy, Berlin, Germany, [Zuberbier, Torsten] Berlin Inst Hlth, Comprehens Allergy Ctr, Dept Dermatol & Allergy, Berlin, Germany, [Nhan Pham-Thi] Pasteur Inst, Allergy Dept, Paris, France, [Agache, Ioana] Transylvania Univ, Fac Med, Brasov, Romania, [Annesi-Maesano, Isabella] INSERM, Depet Inst Pierre Louis Epidemiol & Publ, Epidemiol Allerg & Resp Dis, Paris, France, [Annesi-Maesano, Isabella] Sorbonne Univ, Med Sch St Antoine, Paris, France, [Ansotegui, Ignacio] Hosp Quironsalud Bizkaia, Dept Allergy & Immunol, Erandio, Spain, [Anto, Josep M.] Ctr Res Environm Epidemiol CREAL, ISGlobAL, Barcelona, Spain, [Anto, Josep M.] Univ Pompeu Fabra, Barcelona, Spain, [Anto, Josep M.] CIBERESP, Barcelona, Spain, [Bachert, Claus] Ghent Univ Hosp, ENT Dept, Upper Airways Res Lab, Ghent, Belgium, [Benveniste, Samuel] Broca Hosp, Natl Ctr Expertise Cognit Stimulat CEN STIMCO, Paris, France, [Benveniste, Samuel] PSL Res Univ, Mines ParisTech CRI, Fontainebleau, France, [Bewick, Mike] iQ4U Consultants Ltd, London, England, [Bosnic-Anticevich, Sinthia] Univ Sydney, Woolcock Inst Med Res, Glebe, NSW, Australia, [Bosnic-Anticevich, Sinthia] Woolcock Emphysema Ctr, Glebe, NSW, Australia, [Bosnic-Anticevich, Sinthia] Sydney Local Hlth Dist, Glebe, NSW, Australia, [Brusselle, Guy] Ghent Univ Hosp, Dept Resp Med, Ghent, Belgium, [Calderon, Moses A.] Imperial Coll London, Natl Heart & Lung Inst, London, England, [Usmani, Omar] Imperial Coll London, Natl Heart & Lung Inst, London, England, [Canonica, G. Walter] IRCCS, Humanitas Clin & Res Ctr, Personalized Med Clin Asthma & Allergy, Rozzano, MI, Italy, [Canonica, G. Walter] Humanitas Univ, Dept Biomed Sci, Pieve Emanuele, MI, Italy, [Caraballo, Luis] Univ Cartagena, Inst Immunol Res, Campus Zaragocilla, Cartagena, Colombia, [Caraballo, Luis] Fdn Dev Med & Biol Sci Fundemeb, Cartagena, Colombia, [Cardona, Victoria] Hosp Valle De Hebron, Dept Internal Med, Allergy Sect, Barcelona, Spain, [Cardona, Victoria] ARADyAL Res Network, Barcelona, Spain, [Maria Carriazo, Ana] Reg Minist Hlth Andalusia, Seville, Spain, [Cash, Eugene] Nova Southeastern Univ, Coll Psychol, Ft Lauderdale, FL 33314 USA, [Cash, Eugene] Sch Related Psychol Assessments & Clin Intervent, Ft Lauderdale, FL USA, [Cecchi, Lorenzo] USL Toscana Ctr, SOS Allergol & Clin Immunol, Prato, Italy, [Schunemann, Holger J.] USL Toscana Ctr, SOS Allergol & Clin Immunol, Prato, Italy, [Chu, Derek K.] McMaster Univ, Dept Hlth Res Methods Evidence & Impact, Dept Med, Div Immunol & Allergy, Hamilton, ON, Canada, [Colgan, Elaine] Dept Hlth Social Serv & Publ Safety, Belfast, Antrim, North Ireland, [Costa, Elisio] Univ Porto, UCIBIO, Fac Pharm, REQUIMTE, Porto, Portugal, [Costa, Elisio] Univ Porto, AgeUPNetWork, Competence Ctr Act & Hlth Ageing, Porto, Portugal, [Cruz, Alvaro A.] Univ Fed Bahia, ProAR Nucleo Excelencia Asma, Salvador, BA, Brazil, [Cruz, Alvaro A.] WHO GARD Execut Comm, Salvador, BA, Brazil, [Czarlewski, Wienczyslawa] Med Consulting Czarlewski, Levallois Perret, France, [Durham, Stephen] Imperial Coll London, Natl Heart & Lung Inst, Allergy & Clin Immunol Sect, London, England, [Ebisawa, Motohiro] Natl Hosp Org, Sagamihara Natl Hosp, Clin Res Ctr Allergy & Rheumatol, Sagamihara, Kanagawa, Japan, [Erhola, Marina] Natl Inst Hlth & Welf, Helsinki, Finland, [Fauquert, Jean-Luc] CHU Clermont Ferrand, Hop Estaing, Pole Pediat, Unit Allergol Enfant, Clermont Ferrand, France, [Fokkens, Wytske J.] Amsterdam Univ Med Ctr, Dept Otorhinolaryngol, AMC, Amsterdam, Netherlands, [Fonseca, Joao A.] Univ Porto, Ctr Res Hlth Technol & Informat Syst, Fac Med, CINTESIS, Porto, Portugal, [Fonseca, Joao A.] Medida Lda, Porto, Portugal, [Guldemond, Nick] Erasmus Univ, Inst Hlth Policy & Management iBMG, Rotterdam, Netherlands, [Iinuma, Tomohisa] Chiba Univ Hosp, Dept Otorhinolaryngol, Chiba, Japan, [Okamoto, Yoshitaka] Chiba Univ Hosp, Dept Otorhinolaryngol, Chiba, Japan, [Illario, Maddalena] Campania Reg, Div Hlth Innovat, Naples, Italy, [Illario, Maddalena] Federico II Univ Hosp Naples, R&D, Naples, Italy, [Illario, Maddalena] Federico II Univ Hosp Naples, DISMET, Naples, Italy, [Klimek, Ludger] Ctr Rhinol & Allergol, Wiesbaden, Germany, [Kuna, Piotr] Med Univ Lodz, Barlicki Univ Hosp, Div Internal Med Asthma & Allergy, Lodz, Poland, [Kvedariene, Violeta] Vilnius Univ, Fac Med, Dept Pathol, Inst Biomed Sci, Vilnius, Lithuania, [Kvedariene, Violeta] Fac Med, Inst Clin Med, Clin Chest Dis & Allergol, Vilnius, Lithuania, [Larenas-Linneman, Desiree] Med Sur Clin Fdn & Hosp, Ctr Excellence Asthma & Allergy, Mexico City, DF, Mexico, [Laune, Daniel] KYomed INNOV, Montpellier, France, [Le, Lan T. T.] Univ Med & Pharm, Hochiminh City, Vietnam, [Lourenco, Olga] Univ Beira Interior, Fac Hlth Sci, Covilha, Portugal, [Lourenco, Olga] Univ Beira Interior, Hlth Sci Res Ctr, CICS UBI, Covilha, Portugal, [Malva, Joao O.] Univ Coimbra, Fac Med, Coimbra Inst Clin & Biomed Res iCBR, Coimbra, Portugal, [Malva, Joao O.] Ageing Coimbra EIP AHA Reference Site, Coimbra, Portugal, [Menditto, Enrica] Univ Naples Federico II, Ctr Pharmacoecon, CIRFF, Naples, Italy, [Mullol, Joaquim] Hosp Clin Barcelona, ENT Dept, Rhinol Unit, Barcelona, Spain, [Mullol, Joaquim] Hosp Clin Barcelona, ENT Dept, Smell Clin, Barcelona, Spain, [Mullol, Joaquim] Univ Barcelona, CIBERES, IDIBAPS, Clin & Expt Resp Immunoallergy, Barcelona, Spain, [Munter, Lars] Danish Comm Hlth Educ, Copenhagen East, Denmark, [Papadopoulos, Nikos G.] Univ Manchester, Royal Manchester Childrens Hosp, Div Infect Immun & Resp Med, Manchester, Lancs, England, [Papadopoulos, Nikos G.] Univ Athens, Athens Gen Childrens Hosp P&A Kyriakou, Allergy Dept, Pediat Clin 2, Athens, Greece, [Perala, Maritta] Univ Oulu, Fac Med, Oulu, Finland, [Pfaar, Oliver] Phillipps Univ Marburg, Univ Hosp Marburg, Sect Rhinol & Allergy, Dept Otorhinolaryngol Head & Neck Surg, Marburg, Germany, [Phillips, Abigail] Welsh Govt, Dept Hlth & Social Serv, Cardiff, S Glam, Wales, [Phillips, Jim] Ctr Empowering Patients & Communities, Dublin, Ireland, [Pinnock, Hilary] Univ Edinburgh, Asthma UK Ctr Appl Res, Usher Inst Populat Hlth Sci & Informat, Edinburgh, Midlothian, Scotland, [Quinones-Delgado, Pablo] Agcy Social Serv & Dependency, Reg Govt Equal Social Policies & Conciliat Andalu, Seville, Spain, [Rolland, Christine] Assoc Asthme & Allergie, Paris, France, [Rodts, Ulysse] KanopyMed, Paris, France, [Samolinski, Boleslaw] Med Univ Warsaw, Dept Prevent Environm Hazards & Allergol, Warsaw, Poland, [Sanchez-Borges, Mario] Ctr Medicodocente La Trinidad, Allergy & Clin Immunol Dept, Caracas, Venezuela, [Shamji, Mohamed] Imperial Coll London, Immunomodulat & Tolerance Grp, London, England, [Shamji, Mohamed] Imperial Coll London, Allergy & Clin Immunol, London, England, [Somekh, David] EHFF, Dromahair, Ireland, [Togias, Alkis] NIAID, DAIT, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA, [Toppila-Salmi, Sanna] Helsinki Univ Hosp, Skin & Allergy Hosp, Helsinki, Finland, [Toppila-Salmi, Sanna] Univ Helsinki, Helsinki, Finland, [Tsiligianni, Ioanna] Univ Crete, Fac Med, Dept Social Med, Hlth Planning Unit, Iraklion, Greece, [Tsiligianni, Ioanna] Int Primary Care Resp Grp, Aberdeen, Scotland, [Williams, Sian] Int Primary Care Resp Grp, Aberdeen, Scotland, [Usmani, Omar] Royal Brompton Hosp, Airways Dis Sect, London, England, [Walker, Samantha] Asthma UK, London, England, [Wallace, Dana] Nova Southeastern Univ, Ft Lauderdale, FL 33314 USA, [Valiulis, Arunas] Vilnius Univ, Inst Clin Med, Fac Med, Vilnius, Lithuania, [Valiulis, Arunas] Inst Hlth Sci, Vilnius, Lithuania, [Van der Kleij, Rianne] Leiden Univ, Dept Publ Hlth & Primary Care, Med Ctr, Leiden, Netherlands, [Ventura, Maria Teresa] Univ Bari, Unit Geriatr Immunoallergol, Med Sch, Bari, Italy, [Yorgancioglu, Arzu] Celal Bayar Univ, Fac Med, Dept Pulm Dis, Manisa, Turkey, [Zuberbier, Torsten] Univ Med Berlin, Berlin, Germany, [Zuberbier, Torsten] Free Univ Berlin, Berlin, Germany, [Zuberbier, Torsten] Humboldt Uniersitat Berlin, Berlin, Germany, [Zuberbier, Torsten] GA2LEN, Berlin, Germany, Mylan, GSK, ALK, Novartis, Sanofi, Stallergenes-Greer, Uriach, Health Services Management & Organisation (HSMO), Bousquet, J., Pham-Thi, N., Bedbrook, A., Agache, I., Annesi-Maesano, I., Ansotegui, I., Anto, J. M., Bachert, C., Benveniste, S., Bewick, M., Billo, N., Bosnic-Anticevich, S., Bosse, I., Brusselle, G., Calderon, M. A., Canonica, G. W., Caraballo, L., Cardona, V., Carriazo, A. M., Cash, E., Cecchi, L., Chu, D. K., Colgan, E., Costa, E., Cruz, A. A., Czarlewski, W., Durham, S., Ebisawa, M., Erhola, M., Fauquert, J. -L., Fokkens, W. J., Fonseca, J. A., Guldemond, N., Iinuma, T., Illario, M., Klimek, L., Kuna, P., Kvedariene, V., Larenas-Linneman, D., Laune, D., Le, L. T. T., Lourenco, O., Malva, J. O., Marien, G., Menditto, E., Mullol, J., Munter, L., Okamoto, Y., Onorato, G. L., Papadopoulos, N. G., Perala, M., Pfaar, O., Phillips, A., Phillips, J., Pinnock, H., Portejoie, F., Quinones-Delgado, P., Rolland, C., Rodts, U., Samolinski, B., Sanchez-Borges, M., Schunemann, H. J., Shamji, M., Somekh, D., Togias, A., Toppila-Salmi, S., Tsiligianni, I., Usmani, O., Walker, S., Wallace, D., Valiulis, A., Van der Kleij, R., Ventura, M. T., Williams, S., Yorgancioglu, A., Zuberbier, T., and uBibliorum
Subjects: 0301 basic medicine, Pulmonary and Respiratory Medicine, Allergy, medicine.medical_specialty, Aria, Allergic rhinitis - Asthma - Multimorbidity, Health literacy, Chronic respiratory-diseases, 03 medical and health sciences, 0302 clinical medicine, SDG 3 - Good Health and Well-being, Health care, medicine, Self-management, Global alliance, Intensive care medicine, mHealth, Asthma, Newsletter of GARD Section, business.industry, Environmental exposure, medicine.disease, 3. Good health, Integrated care, 030104 developmental biology, Impact, 030228 respiratory system, 13. Climate action, business
Abstract: In all societies, the burden and cost of allergic and chronic respiratory diseases are increasing rapidly. Most economies are struggling to deliver modern health care effectively. There is a need to support the transformation of the health care system into integrated care with organizational health literacy. MASK (Mobile Airways Sentinel NetworK) (1), a new development of the ARIA (Allergic Rhinitis and its Impact on Asthma) initiative (2), and POLLAR (Impact of Air POLLution on Asthma and Rhinitis, EIT Health) (3), in collaboration with professional and patient organizations in the field of allergy and airway diseases, are proposing real-life ICPs—centred around the patient with rhinitis and using mHealth monitoring of environmental exposure. An expert meeting took place at the Pasteur Institute in Paris, December 3, 2018. The aim was to discuss nextgeneration care pathways following an ongoing political agenda (4,5): (I) patient participation, health literacy and self-care through technology-assisted “patient activation”; (II) implementation of care pathways by pharmacists and (III) Next-generation guidelines assessing the recommendations of GRADE guidelines in rhinitis and asthma using real-world evidence (RWE) assessed by mobile technology. The present document reviews the workshop report and follows on from Part 1.
Published: 2019

9. Nitrogen isotopic fractionation as a biomarker for nitrogen use efficiency in ruminants: a meta-analysis

Author: Ana R.J. Cabrita, Gonzalo Cantalapiedra-Hijar, Isabelle Ortigues-Marty, David Makowski, Long Cheng, Richard J. Dewhurst, Pierre Noziere, Hélène Fouillet, António J.M. Fonseca, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Scotland's Rural College (SRUC), Faculty of Veterinary and Agricultural Science [Melbourne], University of Melbourne, Laboratorio Associado Para a Quimica Verde (LAQV/REQUIMTE), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Agronomie, Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Scotland's Rural College (SCUR), Univ Nova Lisboa, Dept Quim, Fac Ciencias & Tecnol, (LAQV REQUIMTE), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Scotland's Rural College, Faculty of Veterinary and Agricultural Sciences, Univ Nova Lisboa, Dept Quim, Fac Ciencias & Tecnol, ( LAQV REQUIMTE ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, and Physiologie de la Nutrition et du Comportement Alimentaire ( PNCA )
Subjects: 0301 basic medicine, Nitrogen balance, Rumen, Nitrogen, chemistry.chemical_element, Fractionation, ruminant, Biology, [ SDV.BA ] Life Sciences [q-bio]/Animal biology, SF1-1100, nitrogen use efficiency, 03 medical and health sciences, Animal science, Ruminant, Animals, Lactation, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Nitrogen Isotopes, 15N, [SDV.BA]Life Sciences [q-bio]/Animal biology, 0402 animal and dairy science, Regression analysis, 04 agricultural and veterinary sciences, Ruminants, Random effects model, biology.organism_classification, 040201 dairy & animal science, Animal culture, Diet, meta-analysis, 030104 developmental biology, Milk, chemistry, Meta-analysis, Animal Science and Zoology, Animal Nutritional Physiological Phenomena, Digestion, Female, Dietary Proteins, N-15, Biomarkers
Abstract: International audience; Animal proteins are naturally 15N enriched relative to the diet and the extent of this difference (Δ15Nanimal-diet or N isotopic fractionation) has been correlated to N use efficiency (NUE; N gain or milk N yield/N intake) in some recent ruminant studies. The present study used meta-analysis to investigate whether Δ15Nanimal-diet can be used as a predictor of NUE across a range of dietary conditions, particularly at the level of between-animal variation. An additional objective was to identify variables related to N partitioning explaining the link between NUE and Δ15Nanimal-diet. Individual values from eight publications reporting both NUE and Δ15Nanimal-diet for domestic ruminants were used to create a database comprising 11 experimental studies, 41 treatments and individual animal values for NUE (n=226) and Δ15Nanimal-diet (n=291). Data were analyzed by mixed-effect regression analysis taking into account experimental factors as random effects on both the intercept and slope of the model. Diets were characterized according to the INRA feeding system in terms of N utilization at the rumen, digestive and metabolic levels. These variables were used in a partial least squares regression analysis to predict separately NUE and Δ15Nanimal-diet variation, with the objective of identifying common variables linking NUE and Δ15Nanimal-diet. For individuals reared under similar conditions (within-study) and at the same time (within-period), the variance of NUE and Δ15Nanimal-diet not explained by dietary treatments (i.e. between-animal variation plus experimental error) was 35% and 55%, respectively. Mixed-effect regression analysis conducted with treatment means showed that Δ15Nanimal-diet was significantly and negatively correlated to NUE variation across diets (NUE=0.415 -0.055×Δ15Nanimal-diet). When using individual values and taking into account the random effects of study, period and diet, the relationship was also significant (NUE=0.358 -0.035×Δ15Nanimal-diet). However, there may be a biased prediction for animals close to zero, or in negative, N balance. When using a novel statistical approach, attempting to regress between-animal variation in NUE on between-animal variation in Δ15Nanimal-diet (without the influence of experimental factors), the negative relationship was still significant, highlighting the ability of Δ15Nanimal-diet to capture individual variability. Among the studied variables related to N utilization, those concerning N efficiency use at the metabolic level contributed most to predict both Δ15Nanimal-diet and NUE variation, with rumen fermentation and digestion contributing to a lesser extent. This study confirmed that on average Δ15Nanimal-diet can predict NUE variation across diets and across individuals reared under similar conditions.
Published: 2017
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10. Subproteomic signature comparison of in vitro selected fluoroquinolone resistance and ciprofloxacin stress in Salmonella Typhimurium DT104B

Author: Patrícia Poeta, Gilberto Igrejas, Carmen Torres, Manuela Caniça, Ingrid Chafsey, José Luis Capelo, Susana Correia, Christophe Chambon, Didier Viala, Michel Hébraud, department of neurology, clinical dementia center and DZNE, University Medical Centre Goettingen, Microbiologie Environnement Digestif Santé - Clermont Auvergne ( MEDIS ), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne ( UCA ), Qualité des Produits Animaux ( QUAPA ), Institut National de la Recherche Agronomique ( INRA ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Universidad de La Rioja ( UR ), National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, I.P, UCIBIO-REQUIMTE, Faculty of Science and Technology, Universidade Nova de Lisboa, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Department of Genetics and Biotechnology, Department of Genetics and Biotechnology, Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro (Portugal), Veterinary Science Department, Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Requimte, Universidade do Porto [Porto]-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia (FCT NOVA), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Faculdade de Ciências e Tecnologia (FCT NOVA), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Universidade do Porto [Porto]-Departamento de Química (DQ), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA), Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), Université Clermont Auvergne (UCA)-INRA Clermont-Ferrand-Theix, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Área de Bioquímica y Biología Molecular, Universidad de La Rioja (UR), Faculty of Sciences and Technology (FCT NOVA), Universidade Nova de Lisboa (NOVA), Departamento de Química (DQ), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Universidade do Porto [Porto], Faculty of Sciences and Technology, ProteoMass Scientific Society, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Microbiologie Environnement Digestif Santé (MEDIS), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Portuguese Foundation for Science and Technology (FCT) SFRH/BD/75160/2010, European Social Fund Ministry of Education and Science (MEC), national funds from FCT/MEC UID/Multi/04378/2013, ERDF under the PT Partnership Agreement POCI-01-0145-FEDER-007728, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade do Porto-Departamento de Química (DQ), and Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
Subjects: 0301 basic medicine, Salmonella, Proteome, [SDV]Life Sciences [q-bio], [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, education, Shotgun Proteomics, Salmonella Typhimurium DT104B, Biology, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, medicine.disease_cause, fluoroquinolone, Biochemistry, Microbiology, ciprofloxacine, 03 medical and health sciences, stress, Antibiotic resistance, Stress, Physiological, ciprofloxacin, Lc ms ms, Drug Resistance, Bacterial, medicine, antimicrobial resistance, Selection, Genetic, LC-MS/MS, Shotgun proteomics, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Comparative Subproteomics, [ SDV ] Life Sciences [q-bio], Resistência aos Antimicrobianos, comparative subproteomics, in vitro, 2-DE, salmonella Typhimurium DT104B, In vitro, Fluoroquinolone resistance, 3. Good health, Anti-Bacterial Agents, Ciprofloxacin, 030104 developmental biology, shotgun proteomics, Antimicrobial Resistance, salmonella typhimurium, medicine.drug, Fluoroquinolones
Abstract: Background: Fluoroquinolone resistance in nontyphoidal Salmonella is a situation of serious and international concern, particularly in S. Typhimurium DT104B multiresistant strains. Although known to be multifactorial, fluoroquinolone resistance is still far from a complete understanding. Methods: Subproteome changes between an experimentally selected fluoroquinolone-resistant strain (Se6-M) and its parent strain (Se6), and also in Se6-M under ciprofloxacin (CIP) stress, were evaluated in order to give new insights into the mechanisms involved. Proteomes were compared at the intracellular and membrane levels by a 2-DE~LC-MS/MS and a shotgun LC-MS/MS approach, respectively. Results: In total, 35 differentially abundant proteins were identified when comparing Se6 with Se6-M (25 more abundant in Se6 and 10 more abundant in Se6-M) and 82 were identified between Se6-M and Se6-M+CIP (51 more abundant in Se6-M and 31 more abundant under ciprofloxacin stress). Conclusion: Several proteins with known and possible roles in quinolone resistance were identified which provide important information about mechanism-related differential protein expression, supporting the current knowledge and also leading to new testable hypotheses on the mechanism of action of fluoroquinolone drugs. info:eu-repo/semantics/publishedVersion
Published: 2017
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11. Wheat glutenin: the 'tail' of the 1By protein subunits

Author: Emmanuelle Bancel, Gilberto Igrejas, Gérard Branlard, José Luis Capelo, J. D. Nunes-Miranda, Christophe Chambon, Didier Viala, Catherine Ravel, Functional Genomics and Proteomics Unit, Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (Portugal), UCIBIO-REQUIMTE, Faculty of Science and Technology, Universidade Nova de Lisboa, Génétique Diversité et Ecophysiologie des Céréales ( GDEC ), Institut National de la Recherche Agronomique ( INRA ) -Université Blaise Pascal - Clermont-Ferrand 2 ( UBP ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Qualité des Produits Animaux ( QUAPA ), Institut National de la Recherche Agronomique ( INRA ), Faculty of Sciences and Technology, Universidade Nova de Lisboa ( UNINOVA ), Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Requimte, Universidade do Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Portuguese Foundation for Science and Technology (FCT) SFRH/BD/80496/2011, European Social Fund, Ministry of Education and Science (MEC), FCT/MEC UID/Multi/04378/2013, ERDF POCI-01-0145-FEDER-007728, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia (FCT NOVA), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Faculdade de Ciências e Tecnologia (FCT NOVA), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Universidade do Porto [Porto]-Departamento de Química (DQ), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Universidade do Porto [Porto], Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Faculty of Sciences and Technology (FCT NOVA), Universidade Nova de Lisboa (NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Recherche Agronomique (INRA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Universidade do Porto [Porto]-Departamento de Química (DQ), and Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)
Subjects: 0106 biological sciences, 0301 basic medicine, gluténine, Glutens, [SDV]Life Sciences [q-bio], Protein subunit, Biophysics, proteolytic cleavage, 01 natural sciences, Biochemistry, 1By HMW-GS, Polymerization, 03 medical and health sciences, Glutenin, clivage proteolytique, blé, wheat, Immunochemistry, Food Quality, Storage protein, Asparagine, Cysteine, Triticum, wheat glutenin, 2. Zero hunger, chemistry.chemical_classification, C-terminal domain maturation, biology, [ SDV ] Life Sciences [q-bio], asparaginyl endopeptidase, Seed Storage Proteins, food and beverages, asparagine, Gluten, Molecular Weight, Protein Subunits, 030104 developmental biology, Enzyme, chemistry, protéine, biology.protein, protein, Protein Processing, Post-Translational, 010606 plant biology & botany, glutenin
Abstract: Epub ahead of print; Gluten-forming storage proteins play a major role in the viscoelastic properties of wheat dough through the formation of a continuous proteinaceous network. The high-molecular-weight glutenin subunits represent a functionally important subgroup of gluten proteins by promoting the formation of large glutenin polymers through interchain disulphide bonds between glutenin subunits. Here, we present evidences that y-type glutenin subunits encoded at the Glu-B1 locus are prone to proteolytic processing at the C-terminus tail, leading to the loss of the unique cysteine residue present at the C-terminal domain. Results obtained by intact mass measurement and immunochemistry for each proteoform indicate that the proteolytic cleavage appears to occur at the carboxyl-side of two conserved asparagine residues at the C-terminal domain start. Hence, we hypothesize that the responsible enzymes are a class of cysteine endopeptidases - asparaginyl endopeptidases - described in post-translational processing of other storage proteins in wheat. Biological significance The reported study provides new insights into wheat storage protein maturation. In view of the importance of gluten proteins on dough viscoelastic properties and end-product quality, the reported C-terminal domain cleavage of high-molecular-weight glutenin subunits is of particular interest, since this domain possesses a unique conserved cysteine residue which is assumed to participate in gluten polymerization.
Published: 2017
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12. Comparative subproteomic analysis of clinically acquired fluoroquinolone resistance and ciprofloxacin stress in Salmonella Typhimurium DT104B

Author: José Luis Capelo, Susana Correia, Christophe Chambon, Michel Hébraud, Yolanda Sáenz, Gilberto Igrejas, Didier Viala, Ingrid Chafsey, Patrícia Poeta, Functional Genomics and Proteomics Unit, Universidade de Trás-os-Montes e Alto Douro, Department of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro, Veterinary Science Department, University of Trás-os-Montes and Alto Douro (Portugal), Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia (FCT NOVA), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Faculdade de Ciências e Tecnologia (FCT NOVA), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Universidade do Porto [Porto]-Departamento de Química (DQ), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA)-Universidade do Porto [Porto], Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), Université Clermont Auvergne (UCA)-INRA Clermont-Ferrand-Theix, Qualité des Produits Animaux (QuaPA), Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja (CIBIR), Faculty of Sciences and Technology (FCT NOVA), Universidade Nova de Lisboa (NOVA), Portuguese Foundation for Science and Technology (FCT) SFRH/BD/75160/2010, European Social Fund, Ministry of Education and Science (MEC), FCT/MEC UID/Multi/04378/2013, ERDF POCI-01-0145-FEDER-007728, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Microbiologie Environnement Digestif Santé (MEDIS), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), department of neurology, clinical dementia center and DZNE, University Medical Centre Goettingen, Microbiologie Environnement Digestif Santé - Clermont Auvergne ( MEDIS ), Université Clermont Auvergne ( UCA ) -INRA Clermont-Ferrand-Theix, Qualité des Produits Animaux ( QUAPA ), Institut National de la Recherche Agronomique ( INRA ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Centro de Investigación Biomédica de La Rioja, UCIBIO-REQUIMTE, Faculty of Science and Technology, Universidade Nova de Lisboa, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Universidade do Porto [Porto]-Departamento de Química (DQ), Universidade Nova de Lisboa (NOVA)-Universidade Nova de Lisboa (NOVA), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne ( UCA ), Faculty of Sciences and Technology, Universidade Nova de Lisboa ( UNINOVA ), Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
Subjects: 0301 basic medicine, Serotype, Salmonella, fluoroquinolone resistance, salmonella typhimurium DT104B, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, [SDV]Life Sciences [q-bio], Clinical Biochemistry, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, medicine.disease_cause, fluoroquinolone, Microbiology, ciprofloxacine, 03 medical and health sciences, Antibiotic resistance, Stress, Physiological, ciprofloxacin, Drug Resistance, Bacterial, medicine, Humans, antimicrobial resistance, ciprofloxacin stress, ComputingMilieux_MISCELLANEOUS, résistance antimicrobienne, biology, Strain (chemistry), [ SDV ] Life Sciences [q-bio], comparative subproteomics, biology.organism_classification, 3. Good health, Ciprofloxacin, 030104 developmental biology, Mechanism of action, Salmonella enterica, Proteome, medicine.symptom, salmonella typhimurium, medicine.drug
Abstract: PURPOSE Antimicrobial resistance is a worldwide public health threat and Salmonella enterica subsp. enterica serotype Typhimurium phage type DT104B multiresistant strains with additional quinolone resistance have been responsible for global outbreaks and high mortality. Quinolone resistance is known to be multifactorial but is still far from a complete understanding. To give new insights about the resistance mechanisms involved, this work aimed to evaluate subproteome changes between an S. Typhimurium DT104B clinical strain that acquired fluoroquinolone resistance after treatment (Se20) and its pretreatment parental strain (Se6), and also subproteome variations in Se20 under ciprofloxacin (CIP) stress (Se20+CIP). EXPERIMENTAL DESIGN The proteomes were compared at the intracellular and membrane levels by a 2-DE∼LC-MS/MS and a shotgun LC-MS/MS approach, respectively. RESULTS In total, 14 differentially abundant proteins were identified when comparing Se6 with Se20 and 91 were identified between Se20 and Se20+CIP. Several proteins with known and possible roles in quinolone resistance (AAC(6')-Ib-cr4, OmpD, OmpX, GlmS, GlmU, H-NS, etc.) were identified and discussed. CONCLUSIONS AND CLINICAL RELEVANCE The great number of proteins identified in this study provides important information about mechanism-related differential protein expression which supports the current knowledge and might lead to new testable hypotheses on the mechanism of action of fluoroquinolone drugs.
Published: 2017
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13. Structure of a truncated form of leucine zipper II of JIP3 reveals an unexpected antiparallel coiled-coil arrangement

Author: Julie Ménétrey, Paola Llinas, Annélie de Régibus, Mélanie Chenon, Cátia Moreira, Pedro A. Fernandes, T. Quyen Nguyen, Maria J. Ramos, Raphael Guerois, Aline Coquard, Laboratoire d'Enzymologie et Biochimie Structurales (LEBS), Centre National de la Recherche Scientifique (CNRS), Faculty of Science and Technology, BIOSCOPE Group, UCIBIO-REQUIMTE, Universidade de Lisboa (ULISBOA), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, Laboratoire d'Enzymologie et Biochimie Structurales ( LEBS ), Centre National de la Recherche Scientifique ( CNRS ), Universidade de Lisboa ( ULISBOA ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Requimente, Departamento de Química, Faculdade de Ciências, Universidade do Porto [Porto], Universidade de Lisboa = University of Lisbon (ULISBOA), Universidade do Porto = University of Porto-Departamento de Química (DQ), and Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)
Subjects: 0301 basic medicine, Leucine zipper, crystal structure, buried asparagine, Stereochemistry, [SDV]Life Sciences [q-bio], Biophysics, Nerve Tissue Proteins, Small G Protein, Plasma protein binding, Molecular Dynamics Simulation, Crystallography, X-Ray, Antiparallel (biochemistry), Biochemistry, Protein Structure, Secondary, Research Communications, 03 medical and health sciences, Structural Biology, leucine zipper II, Genetics, Molecular motor, Humans, JIP4, Protein Interaction Domains and Motifs, Amino Acid Sequence, Binding site, Structural motif, Adaptor Proteins, Signal Transducing, JIP3, antiparallel coiled coil, Coiled coil, Leucine Zippers, 030102 biochemistry & molecular biology, [ SDV ] Life Sciences [q-bio], Chemistry, Condensed Matter Physics, Peptide Fragments, molecular dynamics, Protein Structure, Tertiary, Crystallography, 030104 developmental biology, Crystallization
Abstract: International audience; JIP3 and JIP4, two highly related scaffolding proteins for MAP kinases, are binding partners for two molecular motors as well as for the small G protein ARF6. The leucine zipper II (LZII) region of JIP3/4 is the binding site for these three partners. Previously, the crystal structure of ARF6 bound to JIP4 revealed LZII in a parallel coiled-coil arrangement. Here, the crystal structure of an N-terminally truncated form of LZII of JIP3 alone shows an unexpected antiparallel arrangement. Using molecular dynamics and modelling, the stability of this antiparallel LZII arrangement, as well as its specificity for ARF6, were investigated. This study highlights that N-terminal truncation of LZII can change its coiled-coil orientation without affecting its overall stability. Further, a conserved buried asparagine residue was pinpointed as a possible structural determinant for this dramatic structural rearrangement. Thus, LZII of JIP3/4 is a versatile structural motif, modifications of which can impact partner recognition and thus biological function.
Published: 2016
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14. Solubility of Bioactive, Inorganic and Polymeric Solids in Ionic Liquids — Experimental and Prediction Perspectives

Author: Luís C. Branco, Małgorzata E. Zakrzewska, Gonçalo V. S. M. Carrera, Ana V.M. Nunes, DQ - Departamento de Química, and CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
Subjects: chemistry.chemical_compound, Materials science, chemistry, Ionic liquid, Organic chemistry, Solubility
Abstract: This work was supported by Fundação para a Ciência e a Tecnologia through projects (PEstC/LA0006/2013, PTDC/CTM/103664/2008, EXPL/QEQ ERQ/2243/2013) one contract under Investigador FCT (L.C. Branco); two Postdoctoral fellowships (G.V.S.M. Carrera - SFRH/BPD/72095/2010 and A. V. M. Nunes - SFRH/BPD/74994/2010) and one doctoral fellowship (M. E.Zakrzewska SFRH/BD/74929/2010). publishersversion published
Published: 2015
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15. Novel markers to early detect degradation on cellulose nitrate-based heritage at the submicrometer level using synchrotron UV–VIS multispectral luminescence

Author: Maria Elvira Callapez, Maria João Melo, Artur Neves, Ana M. Ramos, Mathieu Thoury, Robert Friedel, Matthieu Réfrégiers, LAQV@REQUIMTE, DCR - Departamento de Conservação e Restauro, DQ - Departamento de Química, Refregiers, Matthieu, Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Centro Interuniversitário de História das Ciências e da Tecnologia [Caparica] (CIUHCT), University of Maryland [Baltimore], Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Institut photonique d'analyse non-destructive européen des matériaux anciens (IPANEMA), Muséum national d'Histoire naturelle (MNHN)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme (H2020-NMBP‐35‐2017), Grant agreement no 76081, Project NEMOSINE, 'Innovative packaging solutions for storage and conservation of 20th century cultural heritage of artefacts based on cellulose derivatives, and Fundação para a Ciência e a Tecnologia, Ministério da Ciência Tecnologia e Ensino Superior (FCT/MCTES), Portugal, through doctoral programme CORES-PD/00253/2012, PhD Grant PD/BD/136678/2018 (Artur Neves) and Associate Laboratory for Green Chemistry- LAQV financed by national funds (UIDB/ 50006/2020 and UIDP/50006/2020)., Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), and Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Subjects: Materials science, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Photochemistry, Science, Multispectral image, Nanotechnology, 02 engineering and technology, 01 natural sciences, Article, law.invention, Celluloid, chemistry.chemical_compound, Ultraviolet visible spectroscopy, Nitrate, SDG 3 - Good Health and Well-being, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, History of chemistry, Cellulose, General, Multidisciplinary, 010401 analytical chemistry, [SHS.ART]Humanities and Social Sciences/Art and art history, 021001 nanoscience & nanotechnology, Synchrotron, 0104 chemical sciences, Optics and photonics, Physical chemistry, chemistry, visual_art, visual_art.visual_art_medium, Degradation (geology), Medicine, [SHS.ART] Humanities and Social Sciences/Art and art history, 0210 nano-technology, Luminescence
Abstract: 20200316 H2020-NMBP‐35‐2017 CORES-PD/00253/2012 PD/BD/136678/2018 UIDB/ 50006/2020 UIDP/50006/2020 Cellulose nitrate (CN) is an intrinsically unstable material that puts at risk the preservation of a great variety of objects in heritage collections, also posing threats to human health. For this reason, a detailed investigation of its degradation mechanisms is necessary to develop sustainable conservation strategies. To investigate novel probes of degradation, we implemented deep UV photoluminescence micro spectral-imaging, for the first time, to characterize a corpus of historical systems composed of cellulose nitrate. The analysis of cinematographic films and everyday objects dated from the nineteenth c./early twentieth c. (Perlov's collection), as well as of photo-aged CN and celluloid references allowed the identification of novel markers that correlate with different stages of CN degradation in artworks, providing insight into the role played by plasticizers, fillers, and other additives in stability. By comparison with photoaged references of CN and celluloid (70% CN and 30% camphor), it was possible to correlate camphor concentration with a higher rate of degradation of the cinematographic films. Furthermore, the present study investigates, at the sub-microscale, materials heterogeneity that correlates to the artworks' history, associating the different emission profiles of zinc oxide to specific color formulations used in the late nineteenth and early twentieth centuries. publishersversion published
Published: 2021
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16. Simvastatine confinée dans MCM-41 : désordre dynamique, organisations/interactions moléculaires et leurs implications sur la vitesse de dissolution et le profil de libération

Author: T. Correia, Natália, Cordeiro, Teresa, Affouard, Frédéric, Matos, Ines, Danede, Florence, Sotomayor, João, Corvo, Marta, Fonseca, Isabel, Dionísio, Madalena, Viciosa, Maria T., Unité Matériaux et Transformations - UMR 8207 (UMET), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), CENIMAT/I3N, Departemento de Ciencia dos Materiais, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), and Universidade de Lisboa = University of Lisbon (ULISBOA)
Subjects: [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], [PHYS.COND.CM-DS-NN]Physics [physics]/Condensed Matter [cond-mat]/Disordered Systems and Neural Networks [cond-mat.dis-nn], [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
Abstract: International audience; La simvastatine (SIM) est un principe actif utilisé dans le monde entier pour diminuer le tauxde cholestérol. Dans cette communication, nous présentons une étude détaillée (structure,thermodynamique et mobilité moléculaire) de la simvastatine lorsqu’elle est chargée dans unematrice de silice mésoporeuse MCM-41 (diamètre moyen des pores 3.5 nm) afin de stabiliserl'état amorphe de cette molécule et améliorer sa dissolution dans le milieu aqueux.Les investigations ont été réalisées à partir d’expériences de diffraction des rayons X, résonance magnétique nucléaire, analyse enthalpique différentielle à balayage, ATR-FTIR, spectroscopie de relaxation diélectrique et de simulations numériques de dynamique moléculaire. Nous montrons que les caractéristiques de dissolution et du profil de libération de la simvastatine confinée dans MCM-41(cf. Figure 1) résultent d’une action conjointe complexe de divers facteurs impliquant l’état physique du principe actif, l’organisation structurale des molécules, leur mobilité ainsi que leurs interactions avec la surface interne de la matrice de confinement.
Published: 2022

17. How Molecular Mobility, Physical State, and Drug Distribution Influence the Naproxen Release Profile from Different Mesoporous Silica Matrices

Author: Mirtha A.O. Lourenço, Paula Ferreira, Piedade d’Orey, João Sotomayor, Inês Matos, Teresa Cordeiro, Isabel Fonseca, Natália T. Correia, Madalena Dionísio, Florence Danede, Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Italian Institute of Technology (IIT), Unité Matériaux et Transformations - UMR 8207 (UMET), and Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: Materials science, amorphous, Pharmaceutical Science, 02 engineering and technology, Conductivity, 030226 pharmacology & pharmacy, 03 medical and health sciences, Drug Delivery Systems, 0302 clinical medicine, Differential scanning calorimetry, X-Ray Diffraction, Spectroscopy, Fourier Transform Infrared, Drug Discovery, naproxen, [PHYS.COND.CM-DS-NN]Physics [physics]/Condensed Matter [cond-mat]/Disordered Systems and Neural Networks [cond-mat.dis-nn], Particle Size, mesoporous Silica, Drug Carriers, Calorimetry, Differential Scanning, Hydrogen bond, Water, Mesoporous silica, Silicon Dioxide, 021001 nanoscience & nanotechnology, control release, drug distribution, Solubility, Chemical engineering, Attenuated total reflection, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Molecular Medicine, Adsorption, Crystallization, 0210 nano-technology, Drug carrier, Mesoporous material, Glass transition, Hydrophobic and Hydrophilic Interactions, Porosity, [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]
Abstract: International audience; Aiming to evaluate how the release profile of naproxen (nap) is influenced by its physical state, molecular mobility, and distribution in the host, this pharmaceutical drug was loaded in three different mesoporous silicas differing in their architecture and surface composition. Unmodified and partially silylated MCM-41 matrices, respectively MCM-41 and MCM-41sil, and a biphenylene-bridged periodic mesoporous organic matrix, PMOBph, were synthetized and used as drug carriers, having comparable pore sizes (∼3 nm) and loading percentages (∼30% w/w). The loaded guest was investigated by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, powder X-ray diffraction (XRD), differential scanning calorimetry (DSC), and dielectric relaxation spectroscopy (DRS). DSC and XRD confirmed amorphization of a nap fraction incorporated inside the pores. A narrower glass transition was detected for PMOBph_nap, taken as an indication of the impact of host ordering, which also hinders the guest molecular mobility inside the pores as probed by DRS. While the PMOBph matrix is highly hydrophobic, the unmodified MCM-41 readily adsorbs water, accelerating the nap relaxation rate in the respective composite. In the dehydrated state, the faster dynamics was found for the silylated matrix since guest–host hydrogen bond interactions were inhibited to some extent by methylation. Nevertheless, in all the prepared composites, bulk-like crystalline drug deposits outside pores in a greater extent in PMOBph_nap. The DRS measurements analyzed in terms of conductivity show that, upon melting, nap easily migrates into pores in MCM-41-based composites, while it stays in the outer surface in the ordered PMOBph, determining a faster nap delivery from the latter matrix. On the other side, the mobility enhancement in the hydrated state controls the drug delivery in the unmodified MCM-41 matrix vs the silylated one. Therefore, DRS proved to be a suitable technique to disclose the influence of the ordering of the host surface and its chemical modification on the guest behavior, and, through conductivity depletion, it provides a mean to monitor the guest entrance inside the pores, easily followed even by untrained spectroscopists.
Published: 2021
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18. Cobalt phosphotungstate-based composites as bifunctional electrocatalysts for oxygen reactions

Author: Limani, Ndrina, Marques, Inês, Jarrais, Bruno, Fernandes, António, Freire, Cristina, Fernandes, Diana, LAQV-REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto = University of Porto, Laboratoire Innovation en Chimie des Surfaces et NanoSciences (LICSEN UMR 3685), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), CENIMAT/I3N, Departemento de Ciencia dos Materiais, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), and Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)
Subjects: water oxidation, carbon materials, polyoxometalates, [CHIM.MATE]Chemical Sciences/Material chemistry, N-S-doping, oxygen reduction
Abstract: International audience; The oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) are key reactions in energy-converting systems, such as fuel cells (FCs) and water-splitting (WS) devices. However, the current use of expensive Pt-based electrocatalysts for ORR and IrO$_2$ and RuO$_2$ for OER is still a major drawback for the economic viability of these clean energy technologies. Thus, there is an incessant search for low-cost and efficient electrocatalysts (ECs). Hence, herein, we report the preparation, characterization (Raman, XPS, and SEM), and application of four composites based on doped-carbon materials (CM) and cobalt phosphotungstate (MWCNT_N8_Co4, GF_N8_Co4, GF_ND8_Co4, and GF_NS8_Co4) as ORR and OER electrocatalysts in alkaline medium (pH = 13). Structural characterization confirmed the successful carbon materials doping with N and/or N, S, and the incorporation of the cobalt phosphotungstate. Overall, all composites showed good ORR performance with onset potentials ranging from 0.83 to 0.85 V vs. RHE, excellent tolerance to methanol crossover with current retentions between 88 and 90%, and good stability after 20,000 s at E = 0.55 V vs. RHE (73% to 82% of initial current). In addition, the number of electrons transferred per O$_2$ molecule was close to four, suggesting selectivity to the direct process. Moreover, these composites also presented excellent OER performance with GF_N8_Co4 showing an overpotential of 0.34 V vs. RHE (for j = 10 mA cm$^{-2}$) and jmax close to 70 mA cm$^{-2}$. More importantly, this electrocatalyst outperformed state-of-the-art IrO$_2$ electrocatalyst. Thus, this work represents a step forward toward bifunctional electrocatalysts using less expensive materials.
Published: 2022
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19. Formation of metastable tetragonal zirconia nanoparticles: Competitive influence of the dopants and surface state

Author: Lyubchik, Svetlana [REQUIMTE, Universida de Nova de Lisboa, 2829-516 Caparica (Portugal)]
Published: 2015
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20. Essential Oil Stabilisation by Response Surface Methodology (RSM): Nanoemulsion Formulation, Physicochemical, Microbiological, and Sensory Investigations

Author: Mariem BenJemaa, Fatma Zohra Rahali, Hanen Falleh, Raja Beji-Serairi, Wided Megdiche-Ksouri, Ibtissem Hamrouni, Majdi Hammami, Ralph Nehme, Renato B. Pereira, Sonia Andrés, Said Bouhallab, David M. Pereira, Fabrizio Ceciliani, Latifa Abdennebi-Najar, Riadh Ksouri, Giboulot, Anne, Laboratory of Aromatic and Medicinal Plants, Biotechnology Center of Borj-Cédria BP 901, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de l'élevage (IDELE), REQUIMTE/LAQV, Laboratory of Pharmacognosy, Department of Chemistry, Faculty of Pharmacy, University of Porto, Instituto de Ganadería de Montaña, CSIC-Universidad de León, Department of Veterinary Medicine, Università degli Studi di Milano, Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), This study was supported by the Tunisian Ministry of Higher Education, Scientific Research, Information, and Communication Technologies and was funded under MILKQUA-H2020-PRIMA 2018—Section 2., Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie), European Commission, and Andrés, Sonia
Subjects: essential oil, nanoemulsion, RSM experimental design, physical characterisation, biological activities, sensory analysis, Organic Chemistry, Pharmaceutical Science, Analytical Chemistry, Thymus Plant, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Milk, Anti-Infective Agents, Chemistry (miscellaneous), Drug Discovery, Oils, Volatile, Molecular Medicine, Animals, Emulsions, Physical and Theoretical Chemistry, Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: This manuscript aimed to optimise the encapsulation of Thymus capitatus essential oil into nanoemulsion. Response Surface Methodology results were best fitted into polynomial models with regression coefficient values of more than 0.95. The optimal nanoemulsion showed nanometer-sized droplets (380 nm), a polydispersity index less than 0.5, and a suitable Zeta potential (−10.3 mV). Stability results showed that nanoemulsions stored at 4 °C were stable with the lowest d3,2, PolyDispersity Index (PDI), and pH (day 11). Significant ameliorations in the capacity to neutralise DPPH radical after the encapsulation of the antimicrobial efficacy of thyme essential oil were recorded. S. typhimurium growth inhibition generated by nanoencapsulated thyme essential oil was 17 times higher than by bulk essential oil. The sensory analysis highlighted that the encapsulation of thyme essential oil improved enriched milk’s sensory appreciation. Indeed, 20% of the total population attributed a score of 4 and 5 on the scale used for milk enriched with nanoemulsion. In comparison, only 11% attributed the same score to milk enriched with bulk essential oil. The novel nanometric delivery system presents significant interest for agroalimentary industries., This study was supported by the Tunisian Ministry of Higher Education, Scientific Research, Information, and Communication Technologies and was funded under MILKQUA-H2020-PRIMA 2018—Section 2.
Published: 2022
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21. Pre-miRNA-149 G-quadruplex as a molecular agent to capture nucleolin

Author: Tiago Santos, André Miranda, Lionel Imbert, Andreia Jardim, Catarina R.F. Caneira, Virgínia Chu, João P. Conde, Maria Paula Cabral Campello, António Paulo, Gilmar Salgado, Eurico J. Cabrita, Carla Cruz, University of Beira Interior [Portugal] (UBI), Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento em Lisboa (INESC-ID), Instituto Superior Técnico, Universidade Técnica de Lisboa (IST)-Instituto de Engenharia de Sistemas e Computadores (INESC), Centro de Ciencias e Tecnologias Nucleares, Instituto Superior Tecnico, Universidade de Lisboa, Bobadela LRS, Portugal, Acides Nucléiques : Régulations Naturelle et Artificielle (ARNA), Université de Bordeaux (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), UCIBIO-REQUIMTE, Centro de Investigaçao Em Ciencias da Saúde - Health Sciences Research Center - University of Beira Interior (CICS-UBI), Centro de Investigação em Ciências da Saúde (CICS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB), Thomas, Frank, UCIBIO - Applied Molecular Biosciences Unit, and DQ - Departamento de Química
Subjects: Nucleolin, 0303 health sciences, Molecular receptors, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], RNA-Binding Proteins, Pharmaceutical Science, G-quadruplex ligands, Phosphoproteins, Cell Line, Pre-miRNA-149, G-Quadruplexes, Molecular Docking Simulation, RNA G-quadruplex, MicroRNAs, 03 medical and health sciences, 0302 clinical medicine, 030220 oncology & carcinogenesis, Humans, 030304 developmental biology
Abstract: PD/BD/142851/2018 PD/00065/2013 MIT-EXPL/BIO/0008/2017 IF/00959/2015 One of the most significant challenges in capturing and detecting biomarkers is the choice of an appropriate biomolecular receptor. Recently, RNA G-quadruplexes emerged as plausible receptors due to their ability to recognize with high-affinity proteins. Herein, we have unveiled and characterized the capability of the precursor microRNA 149 to form a G-quadruplex structure and determined the role that some ligands may have in its folding and binding capacity to nucleolin. The G-quadruplex formation was induced by K+ ions and stabilized by ligands, as demonstrated by nuclear magnetic resonance and circular dichroism experiments. Surface plasmon resonance measurements showed a binding affinity of precursor microRNA 149 towards ligands in the micromolar range (10−5–10−6 M) and a strong binding affinity to nucleolin RNA-binding domains 1 and 2 (8.38 × 10−10 M). Even in the presence of the ligand PhenDC3, the binding remains almost identical and in the same order of magnitude (4.46 × 10−10 M). The molecular interactions of the RNA G-quadruplex motif found in precursor miRNA 149 (5′-GGGAGGGAGGGACGGG- 3′) and nucleolin RNA-binding domains 1 and 2 were explored by means of molecular docking and molecular dynamics studies. The results showed that RNA G-quadruplex binds to a cavity between domains 1 and 2 of the protein. Then, complex formation was also evaluated through polyacrylamide gel electrophoresis. The results suggest that precursor microRNA 149/ligands and precursor microRNA 149/nucleolin RNA-binding domains 1 and 2 form stable molecular complexes. The in vitro co-localization of precursor microRNA 149 and nucleolin in PC3 cells was demonstrated using confocal microscopy. Finally, a rapid and straightforward microfluidic strategy was employed to check the ability of precursor microRNA 149 to capture nucleolin RNA-binding domains 1 and 2. The results revealed that precursor microRNA 149 can capture nucleolin RNA-binding domains 1 and 2 labeled with Fluorescein 5-isothiocyanate in a concentration-dependent manner, but PhenDC3 complexation seems to decrease the ability of precursor microRNA 149 to capture the protein. Overall, our results proved the formation of the G-quadruplex structure in the precursor microRNA 149 and the ability to recognize and detect nucleolin. This proof-of-concept study could open up a new framework for developing new strategies to design improved molecular receptors for capture and detection of nucleolin in complex biological samples. publishersversion published
Published: 2021
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22. The SOUL family of heme-binding proteins: Structure and function 15 years later

Author: Jean-Marc Moulis, Susana S. Aveiro, Anjos L. Macedo, Gloria C. Ferreira, Ana Luísa Carvalho, Alastair G. McEwen, Leonildo Delgado, Brian J. Goodfellow, João E. Rodrigues, Filipe Freire, Peggy Charbonnier, Catherine Birck, Pierre Poussin-Courmontagne, CICECO, Departamento de Química, Instituto de Biologia Experimental e Tecnológica (IBET), Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, GreenCoLab - Associação Oceano Verde, Universidade do Algarve, Métaux et Organes (MET&OR), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Laboratory of Fundamental and Applied Bioenergetics = Laboratoire de bioénergétique fondamentale et appliquée (LBFA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Equity Analyst Biotech & Healthcare, Morsani College of Medicine [Tampa, USA], University of South Florida [Tampa] (USF), Center for Neuroscience and Cell Biology (CNC) (CNC), University of Coimbra [Portugal] (UC)-Neuroscience Research Domain, Centre for Integrative Biology - CBI (Inserm U964 - CNRS UMR7104 - IGBMC), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), and Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)
Subjects: Heme binding, Globular protein, Protein Data Bank (RCSB PDB), Inorganic Chemistry, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Protein structure, NMR spectroscopy, Materials Chemistry, HEBP1, [CHIM.COOR]Chemical Sciences/Coordination chemistry, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Physical and Theoretical Chemistry, HEBP2, Function, Heme, Histidine, 030304 developmental biology, X-ray crystallography, chemistry.chemical_classification, 0303 health sciences, Tetrapyrrole binding, Structure, chemistry, SOUL protein, 030220 oncology & carcinogenesis, Biophysics, Heteronuclear single quantum coherence spectroscopy
Abstract: International audience; The SOUL, or heme-binding protein HBP/SOUL, family represents a group of evolutionary conservedputative heme-binding proteins that contains a number of members in animal, plant and bacterial spe-cies. The structures of the murine form of HEBP1, or p22HBP, and the human form of HEBP2, or SOUL,have been determined in 2006 and 2011 respectively.In this work we discuss the structures of HEBP1 and HEBP2 in light of new X-ray data for heme boundmurine HEBP1. The interaction between tetrapyrroles and HEBP1, initially proven to be hydrophobic innature, was thought to also involve electrostatic interactions between heme propionate groups and pos-itively charged amino acid side chains. However, the new X-ray structure, and results from murine HEBP1variants and human HEBP1, confirm the hydrophobic nature of the heme-HEBP1 interaction, resulting inKdvalues in the low nanomolar range, and rules out any electrostatic stabilization. Results from NMRrelaxation time measurements for human HEBP1 describe a rigid globular protein with no change inmotional regime upon heme binding.X-ray structures deposited in the PDB for human HEBP2 are very similar to each other and to the newheme-bound murine HEBP1 X-ray structure (backbone rmsd ca. 1 Å). Results from a HSQC spectrum cen-tred on the histidine side chain Nd-proton region for HEBP2 confirm that HEBP2 does not bind heme viaH42 as no chemical shift differences were observed upon heme addition for backbone NH and Ndprotons.A survey of the functions attributed to HEBP1 and HEBP2 over the last 20 years span a wide range ofcellular pathways. Interestingly, many of them are specific to higher eukaryotes, particularly mammalsand a potential link between heme release under oxidative stress and human HEBP1 is also examinedusing recent data. However, at the present moment, trying to relate function to the involvement of hemehttps://doi.org/10.1016/j.ccr.2021.2141890010-8545/Ó2021 Elsevier B.V. All rights reserved.Abbreviations:HEBP1, Heme-binding protein 1 (or p22HBP); HEBP2, Heme-binding protein 2 (or SOUL); BH3, Bcl-2 homology 3; HSQC, heteronuclear single quantumcoherence spectroscopy; TROSY, transverse relaxation optimized spectroscopy; FQ, fluorescence quenching; PPIX, protoporphyrin IX; sGC, solubleGuanylyl Cyclase; hetNOE,heteronuclear nuclear Overhauser effect; rmsd, root mean squared deviation; BMRB, biological magnetic resonance bank; VAST, vector alignment search tool; MEL, murineerythroleukemia.⇑Corresponding authors.E-mail addresses:brian.goodfellow@ua.pt(B.J. Goodfellow),mdam@fct.unl(A.L. Macedo).Coordination Chemistry Reviews 448 (2021) 214189Contents lists available atScienceDirectCoordination Chemistry Reviewsjournal homepage: www.elsevier.com/locate/ccr
Published: 2021
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23. Ru single atoms and nanoparticles on carbon nanotubes as multifunctional catalysts

Author: Philippe Serp, Diana M. Fernandes, Camila Rivera‐Cárcamo, Mariana Rocha, Cristina Freire, LAQV-REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Laboratoire de chimie de coordination (LCC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, Reducing agent, Inorganic chemistry, Oxygen evolution, Selective catalytic reduction, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Electrochemistry, 01 natural sciences, 7. Clean energy, 0104 chemical sciences, Catalysis, Inorganic Chemistry, chemistry.chemical_compound, Sodium borohydride, Reaction rate constant, chemistry, 13. Climate action, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Methanol, 0210 nano-technology
Abstract: International audience; In the last decade we have witnessed increasing interest in the production of renewable energy and value-added chemicals through sustainable and low-cost technologies where catalysts play a crucial role. Herein, we report the application of a Ru/CNT material containing a mixture of Ru single atoms and Ru nanoparticles as a multifunctional catalyst for both the catalytic reduction of nitroarenes and the electrocatalytic oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). The catalytic activity of the Ru-CNT material was evaluated in the reduction of 4-nitrophenol (4-NP), 4-nitroaniline (4-NA) and 2-nitrophenol (2-NP) in the presence of sodium borohydride as a reducing agent at room temperature, showing high catalytic activity with normalized rate constants (knor) of 19.0 × 103, 57.7 × 103 and 16.6 × 103 min−1 mmol−1 respectively. Furthermore, the catalyst could be reused in at least 10 cycles without catalytic activity loss, confirming the high stability and robustness of the material. The Ru/CNT material also showed good ORR electrocatalytic activity in alkaline medium with Eonset of 0.76 V vs. RHE, a diffusion-limited current density of 3.89 mA cm−2 and ñO2 of 3.3. In addition, Ru/CNT was remarkably insensitive to methanol with a current retention of 93% (51% for Pt/C) and competitive electrochemical stability of 80% after 20 000 s. Moreover, Ru/CNT was active for the OER with jmax = 29.5 mA cm−2 at E = 1.86 V vs. RHE, η10 = 0.50 V and good stability (η10 changed to 0.01 V and jmax only decreased by ≈12% after 500 cycles).
Published: 2020
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24. Photocatalysis Using UV-A and UV-C Light Sources for Advanced Oxidation of Anti-Cancer Drugs Spiked in Laboratory-Grade Water and Synthetic Urine

Author: Patricia Luis, Vanessa J. Pereira, João G. Crespo, Maria Rosário Bronze, Raphael Janssens, Beatriz M. Cristóvão, UCL - SST/IMMC/IMAP - Materials and process engineering, Instituto de Biologia Experimental e Tecnológica - iBET, Faculdade de Farmácia Universidade de Lisboa - iMED, Universidade Nova de Lisboa - LAQV-REQUIMTE, and Universidade Nova de Lisboa - Instituto de Tecnologia Química e Biológica António Xavier
Subjects: Chemistry, General Chemical Engineering, 02 engineering and technology, General Chemistry, 021001 nanoscience & nanotechnology, Photochemistry, Industrial and Manufacturing Engineering, law.invention, Synthetic urine, 020401 chemical engineering, law, Anti cancer drugs, Photocatalysis, 0204 chemical engineering, 0210 nano-technology, Light-emitting diode
Abstract: The presence of anti-cancer drugs in European surface waters appeals for the development of novel treatment processes. In this work, light emitting diodes (LEDs) that emit light at 255 nm (UV-C) and 365 nm (UV-A)wavelengths were compared in terms of their ability to degrade four anti-cancer drugs by UV and UV/TiO2 processes. None of treatments tested was able to degrade cyclophosphamide and ifosfamide. Nevertheless, etoposide and paclitaxel were successfully eliminated by UV-C and UVC/TiO2. Moreover, higher energetic yields were obtained with UV-C light for the degradation of anti-cancer drugs by photocatalysis than with UV-A. The option of using this treatment to deal with pollution at the source by performing essays in synthetic urine was shown to be not adequate, as drug photocatalysis was totally inhibited by the presence of radical scavenger species present in the urine matrix.
Published: 2019
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25. Solvent-Free Microwave Extraction of Thymus mastichina Essential Oil: Influence on Their Chemical Composition and on the Antioxidant and Antimicrobial Activities

Author: Xavier Fernandez, Sandrine Perino, Farid Chemat, Maximiano P. Ribeiro, Paula Coutinho, Cassandra Cunha, Luisa Jordao, Márcio Rodrigues, Jesus M. Rodilla, André R. T. S. Araujo, Lúcia Silva, Polytechnic Institute of Guarda (IPG), REQUIMTE/LAQV, Faculdade de Ciências, Universidade do Porto, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Chimie de Nice (ICN), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Centro de Investigaçao Em Ciencias da Saúde - Health Sciences Research Center - University of Beira Interior (CICS-UBI), Centro de Investigação em Ciências da Saúde (CICS), National Institute of Health Doutor Ricardo Jorge, and Fiber Materials and Environmental Technologies (FibEnTech)
Subjects: 0106 biological sciences, thymus mastichina, Thymus mastichina, Pharmaceutical Science, green extraction, 01 natural sciences, Large Scale, essential oil, law.invention, Steam distillation, chemistry.chemical_compound, Pharmacy and materia medica, 0404 agricultural biotechnology, law, Drug Discovery, Green Extraction, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Essential oil, Solvent-free Microwave Extraction, solvent-free microwave extraction, Chromatography, Chemistry, Extraction (chemistry), 04 agricultural and veterinary sciences, Dry distillation, 040401 food science, Essential Oil, RS1-441, Solvent, Eucalyptol, 13. Climate action, Yield (chemistry), large scale, Medicine, Molecular Medicine, 010606 plant biology & botany
Abstract: This article belongs to the Special Issue Antiradical, Chemopreventive and Antimicrobial Analysis of Bioactive Substances Solvent-free microwave extraction (SFME) is a combination of microwave heating and dry distillation performed at atmospheric pressure without the addition of water or organic solvents that has been proposed as a green method for the extraction of essential oils from aromatic and medicinal herbs. In this work, SFME and the conventional techniques of steam distillation (SD) and hydrodistillation (HD) were compared with respect to the extraction and antioxidant and antimicrobial activities of Thymus mastichina essential oil. The main constituent of essential oils obtained using different methods was 1,8-cineole (eucalyptol). The results showed that the essential oils extracted by means of SFME in 30 min were quantitatively (yield) and qualitatively (aromatic profile) similar to those obtained using conventional HD over 120 min. In addition, SFME generates less waste and less solvent, consumes less energy, and provides a higher yield for a shorter extraction time, which is advantageous for the extraction of the T. mastichina essential oil compared to SD. The antioxidant and antimicrobial activities of the T. mastichina essential oil obtained from either SFME or conventional extraction methods (SD or HD) showed a similar pattern. Large-scale experiments using this SFME procedure showed a potential industrial application. The authors acknowledge the Fundação para a Ciência e Tecnologia (FCT), Fundo Europeu de Desenvolvimento Regional (FEDER), and COMPETE 2020 for their financial support under theresearch project “The development of dermo-biotechnological applications using natural resourcesin the Beira and Serra da Estrela regions—DermoBio”, ref. SAICT-POL/23925/2016, presentedin the Notice for the Presentation of Applications No. 02/SAICT/2016—Scientific Research andTechnological Development Projects (IC & DT) in Co-Promotion (PDF) Solvent-Free Microwave Extraction of Thymus mastichina Essential Oil: Influence on Their Chemical Composition and on the Antioxidant and Antimicrobial Activities. info:eu-repo/semantics/publishedVersion
Published: 2021
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26. Enzymatic synthesis, structures, interactions with saliva proteins and quantification in juices of a series of dehydrodicaffeoylquinic acids, one of the main classes of oxidation products in apple-based beverages

Author: Castillo Fraire, Claudia, Poupard, Pascal, Guilois-Dubois, Sophie, Salas, Erika, Soares, Susana, Brandao, Elsa, de Freitas, Victor, Guyot, Sylvain, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Insitut Français des Productions Cidricoles (IFPC), Universidad Autónoma de Chihuahua (UACH), Requimte, Universidade do Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), and UMT Actia Nova2Cidre (Domaine de la motte)
Subjects: [SDV]Life Sciences [q-bio], [CHIM]Chemical Sciences
Abstract: International audience; Dehydrodicaffeoylquinic acids (DDCQAs) are among the main phenolic products resulting from enzymatic oxidation occurring during apple processing. Accounting for dozen milligrams per liters in some apple juices, they present original chemical structures including benzodioxane, dihydronaphtalene, dihydrobenzofuran or dicatechol patterns. Interestingly, those oxidation products exhibited unusual properties regarding their ability to aggregate salivary proteins.
Published: 2021

27. Successful dissemination of plasmid-mediated extended-Spectrum β-lactamases in enterobacterales over humans to wild fauna

Author: Richard Bonnet, Frédéric Robin, Patrícia Poeta, Racha Beyrouthy, Giberto Igrejas, Carolina Sabença, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Centre National de Référence de la Résistance aux Antibiotiques [CHU Clermont-Ferrand] (CNR), CHU Clermont-Ferrand, Universidade de Trás-os-Montes e Alto Douro (UTAD), Universidade do Trás-os-Montes e Alto Douro, Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto, Ministere de la Recherche et de la Technologie, Inserm (UMR 1071), INRAe (USC-2018), Programa de Accoes Universitarias Integradas Luso-Francesas [TC-14/2017], Portuguese Foundation for Science and Technology [[UID/QUI/50006/2019], ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016), European Project: 323209,EC:FP7:HEALTH,FP7-JPROG-2012-RTD,JPIAMR(2012), Molé, Christine, CAP 20-25 - - CAP 20-252016 - ANR-16-IDEX-0001 - IDEX - VALID, Coordination Action for the early implementation of the Joint Programming Initiative on Antimicrobial Resistance - JPIAMR - - EC:FP7:HEALTH2012-09-01 - 2016-02-29 - 323209 - VALID, Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), and Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)
Subjects: Microbiology (medical), QH301-705.5, medicine.disease_cause, Microbiology, Genome, Article, 03 medical and health sciences, Plasmid, Polymorphism (computer science), Virology, plasmid, medicine, Escherichia coli, IncI1-ST3, CTX-M-1, Biology (General), Gene, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, 030304 developmental biology, Synteny, 2. Zero hunger, Genetics, 0303 health sciences, biology, 030306 microbiology, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, bacterial infections and mycoses, β-lactamase, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Multilocus sequence typing, beta-lactamase, Bacteria
Abstract: Background: The emergence of multidrug-resistant bacteria remains poorly understood in the wild ecosystem and at the interface of habitats. Here, we explored the spread of Escherichia coli containing IncI1-ST3 plasmid encoding resistance gene cefotaximase-Munich-1 (blaCTX-M-1) in human-influenced habitats and wild fauna using a genomic approach. Methods. Multilocus sequence typing (MLST), single-nucleotide polymorphism comparison, synteny-based analysis and data mining approaches were used to analyse a dataset of genomes and circularised plasmids. Results. CTX-M-1 E. coli sequence types (STs) were preferentially associated with ecosystems. Few STs were shared by distinct habitats. IncI1-ST3-blaCTX-M-1 plasmids are disseminated among all E. coli phylogroups. The main divergences in plasmids were located in a shuffling zone including blaCTX-M-1 inserted in a conserved site. This insertion hot spot exhibited diverse positions and orientations in a zone-modulating conjugation, and the resulting synteny was associated with geographic and biological sources. Conclusions. The ecological success of IncI1-ST3-blaCTX-M-1 appears less linked to the spread of their bacterial recipients than to their ability to transfer in a broad spectrum of bacterial lineages. This feature is associated with the diversity of their shuffling conjugation region that contain blaCTX-M-1. These might be involved in the resistance to antimicrobials, but also in their spread.
Published: 2021
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28. Exhaustive reanalysis of barcode sequences from public repositories highlights ongoing misidentifications and impacts taxa diversity and distribution

Author: Marie-Mathilde Perrineau, Isabel C. Azevedo, Claire M. M. Gachon, Philip D. Kerrison, Willem de Visser, Isabel Sousa-Pinto, Kévin Cascella, Philippe Potin, Elisabete da Costa, Ricardo Calado, Johanna E. Beniers, Maria do Rosário Domingues, Ronan Sulpice, Michael D. Guiry, Adrie van der Werf, Antoine Fort, Marcus McHale, Henrice M. Jansen, National University of Ireland [Galway] (NUI Galway), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Scottish Association for Marine Science (SAMS), Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Universidade do Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Centro de Estudos do Ambiante e do Mar (CESAM), Universidade de Aveiro, Interdisciplinary Centre of Marine and Environmental Research [Matosinhos, Portugal] (CIIMAR), Universidade do Porto, Molécules de Communication et Adaptation des Micro-organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research [Wageningen] (WUR), and Martin Ryan Institute
Subjects: Land Use and Food Security, Aquaculture, phylogeny, Barcode, DNA barcoding, law.invention, Onderz. Form. D, Ulva, Aquaculture and Fisheries, law, DNA BArcoding, Genetics, Sea lettuce, 14. Life underwater, Applied Ecology, Ecology, Evolution, Behavior and Systematics, Phylogeny, Geography, biology, Aquacultuur en Visserij, Ulvophyceae, Toegepaste Ecologie, Species diversity, Landgebruik en Voedselzekerheid, biology.organism_classification, Europe, Ulvales, Herbarium, Taxon, aquaculture, Evolutionary biology, [SDE]Environmental Sciences, Biotechnology
Abstract: Accurate species identification often relies on public repositories to compare the barcode sequences of the investigated individual(s) with taxonomically assigned sequences. However, the accuracy of identifications in public repositories is often questionable, and the names originally given are rarely updated. For instance, species of the Sea Lettuce (Ulva spp.; Ulvophyceae, Ulvales, Ulvaceae) are frequently misidentified in public repositories, including herbaria and gene banks, making species identification based on traditional barcoding unreliable. We DNA barcoded 295 individual distromatic foliose strains of Ulva from the North-East Atlantic for three loci (rbcL, tufA, ITS1). Seven distinct species were found, and we compared our results with all worldwide Ulva spp. sequences present in the NCBI database for the three barcodes rbcL, tufA and the ITS1. Our results demonstrate a large degree of species misidentification, where we estimate that 24%–32% of the entries pertaining to foliose species are misannotated and provide an exhaustive list of NCBI sequences reannotations. An analysis of the global distribution of registered samples from foliose species also indicates possible geographical isolation for some species, and the absence of U.lactuca from Northern Europe. We extended our analytical framework to three other genera, Fucus, Porphyra and Pyropia and also identified erroneously labelled accessions and possibly new synonymies, albeit less than for Ulva spp. Altogether, exhaustive taxonomic clarification by aggregation of a library of barcode sequences highlights misannotations and delivers an improved representation of species diversity and distribution.
Published: 2021
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29. Identification of azaphilone derivatives of Monascus colorants from Talaromyces amestolkiae and their halochromic properties

Author: Valéria de Carvalho Santos Ebinuma, Eduardo J. Crevelin, Diana C. G. A. Pinto, Sónia P. M. Ventura, Fernanda Garcia de Oliveira, Inês L.D. Rocha, André Gonzaga dos Santos, Universidade Estadual Paulista (UNESP), University of Aveiro, LAQV - REQUIMTE, and Universidade de São Paulo (USP)
Subjects: Talaromyces amestolkiae, genetic structures, Monascus azaphilones, Halochromism, Red Color, Analytical Chemistry, Hydrolysis, Organic chemistry, Benzopyrans, Red colorants, chemistry.chemical_classification, biology, Extraction (chemistry), Fatty acid, General Medicine, Pigments, Biological, Halocromic properties, Monascus, biology.organism_classification, Color stability, chemistry, Talaromyces, Polyketides, Fermentation, Food Science
Abstract: Made available in DSpace on 2022-04-29T08:34:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-15 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Laboratório Associado para a Química Verde Centro de Investigação em Materiais Cerâmicos e Compósitos Currently, the ability to produce several kinds of water-soluble red natural colorants makes the genus Talaromyces particularly important to the dye industry, which can be an alternative to the use of harmful synthetic colorants. In this study, colored compounds produced by Talaromyces amestolkiae were extracted, characterized chemically and the color stability of the fermented broth without any extraction procedure was further evaluated over pH variation. Five azaphilones compounds were detected by Ultrahigh Performance Liquid Chromatography-Mass Spectrometry system, all being complexes of the fatty acid amino-hexanedioic acid and azaphilone Monascus colorants. The color of the fermented broth was stable at a wide range of pH (3–9). Furthermore, T. amestolkiae colorants precipitated through hydrolysis of key chemical groups at extremely acidic (pH 1) and lose red color in extremely basic (pH 13) medium, showing negative halochromism. Nevertheless, these findings enhance the industrial relevance of azaphilone colorants produced by biotechnological process. Department of Engineering Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University - UNESP CICECO – Aveiro Institute of Materials Chemistry Department University of Aveiro, Campus Universitário de Santiago LAQV - REQUIMTE Department of Chemistry and University of Aveiro Department of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University - UNESP Laboratory of Mass Spectrometry Applied to Natural Products Chemistry Department of Chemistry Faculty of Philosophy Sciences and Letters of Ribeirão Preto (FFCLRP) University of São Paulo (USP) Department of Engineering Bioprocess and Biotechnology School of Pharmaceutical Sciences São Paulo State University - UNESP Department of Drugs and Medicines School of Pharmaceutical Sciences São Paulo State University - UNESP FAPESP: 2014/01580-3 FAPESP: 2018/06908-8 FAPESP: 2019/15493-9 Laboratório Associado para a Química Verde: UIDB/50006/2020 Centro de Investigação em Materiais Cerâmicos e Compósitos: UIDB/50011/2020 Centro de Investigação em Materiais Cerâmicos e Compósitos: UIDP/50011/2020
Published: 2021

30. Heterogeneity of the pharmacologic treatment of allergic rhinitis in Europe based on MIDAS and OTCims platforms

Author: Torsten Zuberbier, Julia Saueressig, Joaquim Mullol, Glenis Scadding, João Fonseca, G. Walter Canonica, Eric Van Ganse, Claus Bachert, Bolesław Samoliński, Olga Lourenço, Oliver Pfaar, Philippe Devillier, Ann-Kathrin Stroh, Wienczyslawa Czarlewski, Jean Bousquet, Ludger Klimek, Elísio Costa, Piotr Kuna, Detlef Schroder-Bernhardi, Victoria Cardona, Sophie Scheire, Nhan Pham Thi, Institut Català de la Salut, [Bousquet J] Charité Universitätsmedizin Berlin, Humboldt-Universität zu Berlin, Berlin, Germany. Department of Dermatology and Allergy, Comprehensive Allergy Center, Berlin Institute of Health, Berlin, Germany. University Hospital Montpellier, Montpellier, France. MACVIA-France, Montpellier, France. [Schröder-Bernhardi D] IQVIA Consumer Health, Frankfurt, Germany. [Bachert C] Upper Airways Research Laboratory, ENT Dept, Ghent University Hospital, Ghent, Belgium. International Airway Research Center, First Affiliated Hospital Guangzou, Sun Yat-sen University, Guangzou, China. Division of ENT Diseases, CLINTEC, Karolinska Institutet, Stockholm, Sweden. Department of ENT Diseases, Karolinska University Hospital, Stockholm, Sweden. [Canonica GW] Personalized Medicine Asthma, & Allergy Clinic-Humanitas University & Research Hospital, IRCCS-Milano, Milano, Italy. [Cardona V] Servei d’Al·lergologia, Servei de Medicina Interna, Vall d'Hebron Hospital Universitari, Barcelona, Spain. ARADyAL Research Network, Barcelona, Spain. [Costa EM] UCIBIO, REQUINTE, Faculty of Pharmacy and Competence Center on Active and Healthy Ageing of University of Porto (Porto4Ageing), Porto, Portugal, Vall d'Hebron Barcelona Hospital Campus, uBibliorum, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Berlin Institute of Health (BIH), Contre les MAladies Chroniques pour un VIeillissement Actif en Languedoc-Roussillon (MACVIA-LR), Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Nîmes (CHU Nîmes)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-European Innovation Partnership on Active and Healthy Ageing Reference Site (EIP on AHA), Commission Européenne-Commission Européenne-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), IQVIA, Ghent University Hospital, Sun Yat-Sen University [Guangzhou] (SYSU), Karolinska Institutet [Stockholm], Karolinska University Hospital [Stockholm], IRCCS Istituto Nazionale dei Tumori [Milano], Vall d'Hebron University Hospital [Barcelona], Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Requimte, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Hôpital Foch [Suresnes], Universidade do Porto = University of Porto, University Medical Center [Mainz], Medical University of Łódź (MUL), University of Beira Interior [Portugal] (UBI), Centro de Investigación Biomédica en Red Enfermedades Respiratorias (CIBERES), Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona (UB), Philipps Universität Marburg = Philipps University of Marburg, Institut de Recherche Biomédicale des Armées (IRBA), Medical University of Warsaw - Poland, University College of London [London] (UCL), Health Service and Performance Research (HESPER), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, and Retiveau, Nolwenn
Subjects: 0301 basic medicine, Allergy, IMPACT, Database analysis, Medications, costs, MESH: Allergy and Immunology, Medication, Other subheadings::Other subheadings::/drug therapy [Other subheadings], Allergic rhinitis, 0302 clinical medicine, medications, MESH: Anti-Allergic Agents, Anti-Allergic Agents, Medicine and Health Sciences, Medicine, Immunology and Allergy, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Practice Patterns, Physicians', health care economics and organizations, International market, Medication use, Units, enfermedades respiratorias::enfermedades nasales::rinitis::rinitis alérgica [ENFERMEDADES], Medicaments - Prescripció - Europa, Europe, MESH: Rhinitis, Allergic, [SDV.IMM]Life Sciences [q-bio]/Immunology, preparados farmacéuticos::medicamentos por prescripción [COMPUESTOS QUÍMICOS Y DROGAS], localizaciones geográficas::Europa (continente) [DENOMINACIONES GEOGRÁFICAS], MIDAS, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, Rinitis - Tractament - Europa, Healthcare system, medicine.medical_specialty, [SDV.IMM] Life Sciences [q-bio]/Immunology, Immunology, Respiratory Tract Diseases::Nose Diseases::Rhinitis::Rhinitis, Allergic [DISEASES], Otros calificadores::Otros calificadores::/farmacoterapia [Otros calificadores], Geographic Locations::Europe [GEOGRAPHICALS], Eu countries, PERSON-CENTERED CARE, Pharmacological treatment, 03 medical and health sciences, units, Environmental health, Allergy and Immunology, MANAGEMENT, Humans, Pharmaceutical Preparations::Prescription Drugs [CHEMICALS AND DRUGS], MESH: Humans, allergic rhinitis, OTC Medicines, business.industry, PATHWAYS, Al·lèrgia - Tractament - Europa, AIR-POLLUTION, medicine.disease, Rhinitis, Allergic, Costs, 030104 developmental biology, 030228 respiratory system, Analytics, Family medicine, MESH: Practice Patterns, Physicians', ASTHMA, MESH: Europe, business
Abstract: Rinitis al·lèrgica; Costos; Medicaments Rinitis alérgica; Costos; Medicamentos Allergic rhinitis; Costs; Medications Background The practice of allergology varies widely between countries, and the costs and sales for the treatment of rhinitis differ depending on practices and health systems. To understand these differences and their implications, the rhinitis market was studied in some of the EU countries. Methods We conducted a pharmaco-epidemiological database analysis to assess the medications that were being prescribed for allergic rhinitis in the years 2016, 2017 and 2018. We used the IQVIA platforms for prescribed medicines (MIDAS®—Meaningful Integration of Data, Analytics and Services) and for OTC medicines (OTC International Market Tracking—OTCims). We selected the five most important markets in the EU (France, Germany, Italy, Poland and Spain). Results Intranasal decongestants were excluded from the analyses because they are rarely prescribed for allergic rhinitis. For both Standard Units (SU) and costs, France is leading the other countries. In terms of SU, the four other countries are similar. For costs, Poland is lower than the three others. However, medication use differs largely. For 2018, in SU, intranasal corticosteroid is the first treatment in Poland (70.0%), France (51.3%), Spain (51.1%) and Germany (50.3%), whereas the Italian market is dominated by systemic antihistamines (41.4%) followed by intranasal corticosteroids (30.1%). Results of other years were similar. Discussion There are major differences between countries in terms of rhinoconjunctivitis medication usage.
Published: 2021
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31. OrpR is a σ 54 ‐dependent activator using an iron‐sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough

Author: Alain Dolla, Zorah Dermoun, Danaé Eve, Corinne Aubert, Mériem Merrouch, Bénédicte Burlat, Anouchka Fiévet, Sofia R. Pauleta, Emanuele G. Biondi, Odile Valette, Gaël Brasseur, Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Departamento de Quimica (REQUIMTE), Universidade de Lisboa (ULISBOA)-Centro de Quimica Fina e Biotecnologia, Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN), Bioénergétique et Ingénierie des Protéines (BIP ), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), ANR-12-ISV8-0003,ORPANADIV,Caractérisation fonctionnelle et structurale de métalloprotéines conservées de fonction inconnue issues d'anaérobes: implication dans le contrôle de la division cellulaire(2012), DQ - Departamento de Química, UCIBIO - Applied Molecular Biosciences Unit, Universidade de Lisboa = University of Lisbon (ULISBOA)-Centro de Quimica Fina e Biotecnologia, and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)
Subjects: enhancer binding protein, Iron–sulfur cluster, Biology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, PAS domain, Transcription (biology), Enhancer binding, Desulfovibrio vulgaris, Molecular Biology, transcriptional regulator, 030304 developmental biology, iron-sulfur protein, [SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere, 0303 health sciences, 030306 microbiology, anaerobes, biology.organism_classification, Desulfovibrio, redox sensor, DNA binding site, Regulon, chemistry, Biochemistry
Abstract: Funding Information: We gratefully acknowledge the contribution of Marielle Bauzan for the cultures in syntrophy and Yann Denis for transcriptomic facilities and Grant Zane (Judy Wall laboratory) for providing us the GZ3621Orp ::mini‐Tn5 strain. The authors are also grateful to Emilien Etienne and the EPR facilities available at the national EPR network RENARD (IR CNRS 3443) and the Aix‐Marseille University EPR center. We also thank Martine Company for technical assistance, Katia Villion for her help on the project during its school program, Béatrice Py and Bruno Guigliarelli for insightful discussions. The French National Research Agency (ANR) funded this research project (ANR‐12‐ISV8‐0003‐01). The project leading to this publication has received funding from the Excellence Initiative of Aix‐Marseille University—A*MIDEX, a French “Investissements d'Avenir” programme and is part of the Institute of Microbiologies and Biotechnology—IM2B (AMX‐19‐IET‐006). This work was supported by Fundação para a Ciência e Tecnologia (FCT) (grant to SRP, FCT‐ANR/BBB‐MET/0023/2012) and by the Belgian Federal Science Policy Office (Belspo) (IAP7/44, iPROS project). SRP was also supported by the Applied Molecular Biosciences Unit‐UCIBIO financed by national funds from FCT (UIDP/04378/2020 and UIDB/04378/2020). This article is based upon work from COST Action CA15133, supported by COST (European Cooperation in Science and Technology). Made available in DSpace on 2021-11-29T23:39:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07 publishersversion published
Published: 2021
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32. Essential Oils in Livestock: From Health to Food Quality

Author: Riadh Ksouri, Ralph Nehme, Meriem Ben Jemaa, Renato B. Pereira, Saïd Bouhallab, Latifa Abdennebi-Najar, Sonia Andrés, Secundino López, Fatma Zohra Rahali, David M. Pereira, Fabrizio Ceciliani, Institut de l'élevage (IDELE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Science et Technologie du Lait et de l'Oeuf (STLO), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universidad de León [León], Departamento de Quimica (REQUIMTE), Universidade de Lisboa (ULISBOA)-Centro de Quimica Fina e Biotecnologia, Centre de Biotechnologie de Borj Cédria (Hammam-Lif, Tunisie), Università degli Studi di Milano [Milano] (UNIMI), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), This research was funded under H2020-PRIMA 2018—Section, European Commission, Andrés, Sonia [0000-0001-5255-1582], López, Secundino [0000-0001-6267-683X], Gestionnaire, Hal Sorbonne Université, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidade de Lisboa = University of Lisbon (ULISBOA)-Centro de Quimica Fina e Biotecnologia, Università degli Studi di Milano = University of Milan (UNIMI), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Andrés, Sonia, and López, Secundino
Subjects: 0301 basic medicine, Livestock, antioxidant, Physiology, [SDV]Life Sciences [q-bio], Clinical Biochemistry, Mastitis, Review, Biology, mastitis, Biochemistry, 03 medical and health sciences, Molecular Biology, essential oils, fermentation, Nutrition, Inflammation, 2. Zero hunger, Animal health, business.industry, Animal product, lcsh:RM1-950, Immunity, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Cell Biology, 040201 dairy & animal science, immunity, Early life, 3. Good health, Biotechnology, [SDV] Life Sciences [q-bio], livestock, lcsh:Therapeutics. Pharmacology, 030104 developmental biology, One Health, nutrition, Essential oils, inflammation, Fermentation, antimicrobial, encapsulation, Antimicrobial, Encapsulation, Antioxidant, business, Food quality
Abstract: 36 páginas, 6 figuras, 4 tablas., Using plant essential oils (EOs) contributes to the growing number of natural plants’ applications in livestock. Scientific data supporting the efficacy of EOs as anti-inflammatory, antibacterial and antioxidant molecules accumulates over time; however, the cumulative evidence is not always sufficient. EOs antioxidant properties have been investigated mainly from human perspectives. Still, so far, our review is the first to combine the beneficial supporting properties of EOs in a One Health approach and as an animal product quality enhancer, opening new possibilities for their utilization in the livestock and nutrition sectors. We aim to compile the currently available data on the main anti-inflammatory effects of EOs, whether encapsulated or not, with a focus on mammary gland inflammation. We will also review the EOs’ antioxidant activities when given in the diet or as a food preservative to counteract oxidative stress. We emphasize EOs’ in vitro and in vivo ruminal microbiota and mechanisms of action to promote animal health and performance. Given the concept of DOHaD (Developmental Origin of Health and Diseases), supplementing animals with EOs in early life opens new perspectives in the nutrition sector. However, effective evaluation of the significant safety components is required before extending their use to livestock and veterinary medicine., This research was funded under H2020-PRIMA 2018—Section 2.
Published: 2021
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33. Electrochemical Activity of Cytochrome P450 1A2: The Relevance of O 2 Control and the Natural Electron Donor

Author: Wissam Ghach, Mathieu Etienne, Sofia A. Pereira, Célia M. Silveira, Patricia Rodrigues, Francisco de Assis Esteves, Michel Kranendonk, M. Gabriela Almeida, Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, Instituto de Tecnologia Química e Biológica António Xavier (ITQB), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), School of Medicine [Cardiff], Cardiff University-Institute of Medical Genetics [Cardiff], Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Centro de Estudos de Doenças Crónicas (CEDOC), NOVA Medical School - Faculdade de Ciências Médicas (NMS), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), and Center for Toxicogenomics and Human Health, Genetics, Oncology and Human Toxicology
Subjects: 0303 health sciences, biology, Cytochrome p450 oxidoreductase, CYP1A2, Cytochrome P450, O2 reduction, Electron donor, 010402 general chemistry, Electrochemistry, 01 natural sciences, Combinatorial chemistry, Catalysis, 0104 chemical sciences, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, 03 medical and health sciences, chemistry.chemical_compound, chemistry, biology.protein, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology
Abstract: International audience
Published: 2021
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34. Continuous electroconversion of CO2 into formate using 2 nm tin oxide nanoparticles

Author: Vincent Vivier, Olivier Durupthy, Ivan Merino-Garcia, Angel Irabien, Lionel Tinat, Carlos M. Sánchez-Sánchez, Jonathan Albo, Manuel Alvarez-Guerra, Universidad de Cantabria, Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Department of Chemical and Biomolecular Engineering, Universidad de Cantabria [Santander], Laboratoire Interfaces et Systèmes Electrochimiques (LISE), and MATISSE
Subjects: Materials science, SnO 2 nanoparticles, Inorganic chemistry, CO2 electroreduction, Nanoparticle, 02 engineering and technology, 010402 general chemistry, Electrocatalyst, 01 natural sciences, CO 2 electroreduction, Catalysis, law.invention, chemistry.chemical_compound, law, formate, [CHIM]Chemical Sciences, Continuous reactor, Formate, General Environmental Science, Electrolysis, Process Chemistry and Technology, SnO2 nanoparticles, 021001 nanoscience & nanotechnology, Tin oxide, 0104 chemical sciences, chemistry, Cyclic voltammetry, Gas diffusion electrodes, 0210 nano-technology, Faraday efficiency
Abstract: Tin oxide nanoparticles (SnO2 NPs) as electrocatalyst for the production of formate from CO2 reduction reaction (CO2RR). We synthesize, characterize and evaluate high surface area SnO2 NPs (2.4 nm and 299 m2 g-1 in diameter size and surface area, respectively), for the continuous production of formate at high current density within a flow electrolyzer. SnO2 NPs under Ar and CO2 reduction conditions were studied by cyclic voltammetry. SnO2-based gas diffusion electrodes (SnO2-GDEs) were manufactured to perform continuous CO2RR. A maximum formate concentration value of 27 g L-1 was achieved with a Faradaic efficiency (FE) of 44.9 % at 300 mA cm-2, which was significantly stable and reproducible when operated up to 10 h. Nevertheless, ohmic drop contribution due to the semiconducting properties of SnO2 was not negligible. The low total FE (< 60 %) of products pointed out a leakage of formate by crossover migration through the membrane from the catholyte towards the anolyte. The authors gratefully acknowledge the financial sources from the Spanish Ministry of Economy and Competitiveness (MINECO), through the project CTQ2016-76231-C2-1-R (AEI/FEDER, UE). Moreover, I.M.- G. and C.M. S.-S would like to thank the MINECO for the postdoctoral period in Paris of the predoctoral research contract (BES-2014-070081) and J. A. for the Ramón y Cajal programme (RYC-2015-17080), respectively. L.T. and O.D. acknowledge the support of French governmental funds managed by the ANR within the Investissements d’Avenir programme under reference ANR-11-IDEX-0004-02, and more specifically within the framework of the Cluster of Excellence MATISSE led by Sorbonne Universit´e. Dr. G. Gouget is strongly acknowledge for HR-TEM images.
Published: 2021
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35. Multidrug resistance dissemination in Escherichia coli Isolated from wild animals: bacterial clones and plasmid complicity

Author: Gilberto Igrejas, Richard Bonnet, Patrícia Poeta, Racha Beyrouthy, Frédéric Robin, Carolina Sabença, University of Trás-os-Montes and Alto Douro [Portugal] (UTAD), LAQV-REQUIMTE, Universidade NOVA de Lisboa, Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Centre National de Référence de la Résistance aux Antibiotiques [CHU Clermont-Ferrand] (CNR), CHU Clermont-Ferrand, Ministère de la Recherche et de la Technologie, INSERM, INRAE, Santé Publique France, Centre Hospitalier Régional Universitaire de Clermont-Ferrand, Portuguese Foundation for Science and Technology [TC-14/2017], [FCT/MCTES (UID/QUI/50006/2019)], ANR-16-IDEX-0001,CAP 20-25,CAP 20-25(2016), and European Project: 323209,EC:FP7:HEALTH,FP7-JPROG-2012-RTD,JPIAMR(2012)
Subjects: Microbiology (medical), antibiotic resistance, wild fauna, Context (language use), Biology, medicine.disease_cause, Microbiology, Genome, 03 medical and health sciences, Antibiotic resistance, Plasmid, plasmid, medicine, Escherichia coli, animal, CTX-M-1, Molecular Biology, Gene, 030304 developmental biology, MESH: alpha-Linolenic Acid / pharmacology, Whole genome sequencing, Genetics, 0303 health sciences, 030306 microbiology, Multiple drug resistance, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ESBL, environment, enterobacteriaceae
Abstract: International audience; Objectives. Epidemiological data concerning third-generation cephalosporin (3GC) resistance in wild fauna are scarce. The aim of this study was to characterize the resistance genes, their genetic context, and clonal relatedness in 17 Escherichia coli resistant to 3GC isolated from wild animals. Methods. The isolates were characterized by short-read whole genome sequencing, and long-read sequencing was used for the hybrid assembly of plasmid sequences. Results. The 3GC resistance gene most identified in the isolates was the extended-spectrum β-lactamases (ESBL)-encoding gene blaCTX-M-1 (82.3%), followed by blaCTX-M-32 (5.9%), blaCTX-M-14 (5.9%), and blaSHV-12 (5.9%). E. coli isolates mainly belonged to the sequence types (STs) rarely reported from humans. The single nucleotide polymorphism (SNP)-based typing showed that most E. coli genomes from wild animals (wild boars, birds of prey, and buzzards) formed clonal clusters (
Published: 2021
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36. Ibuprofen incorporated into unmodified and modified mesoporous silica: From matrix synthesis to drug release

Author: Sara Inocêncio, Teresa Cordeiro, Natália T. Correia, João Sotomayor, Inês Matos, Madalena Dionísio, Marta C. Corvo, Florence Danede, Isabel Fonseca, Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), CENIMAT/I3N, Departemento de Ciencia dos Materiais, Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Associate Laboratory for Green Chemistry LAQV by national funds from FCT/MEC UID/QUI/50006/2019, ERDF under the PT2020 Partnership Agreement POCI-01-0145-FEDER - 007265, Interreg 2 Seas program 2014-2020 - European Regional Development Fund (FEDER) 2S01-059_IMODE, program PHC PESSOA 2018 project 4340/40868R, Portuguese Foundation for Science and TechnologyEuropean CommissionSFRH/BD/114653/2016, Portuguese Foundation for Science and TechnologyEuropean CommissionIF/01242/2014/CP1224/CT0008ROTEIRO/0031/2013 -PINFRA/22161/2016, FEDER through COMPETE 2020, POCI, PORL, FCT through PIDDAC POCI-01-0145-FEDER-007688UID/CTM/50025/2020-2023, Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), and Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: Dielectric spectroscopy, Population, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Amorphous materials, Magic angle spinning nuclear magnetic spectroscopy, Adsorption, General Materials Science, [PHYS.COND.CM-DS-NN]Physics [physics]/Condensed Matter [cond-mat]/Disordered Systems and Neural Networks [cond-mat.dis-nn], education, education.field_of_study, Aqueous solution, Chemistry, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, Mesoporous silica, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Chemical engineering, Mechanics of Materials, Drug delivery, Surface modification, 0210 nano-technology, Glass transition, Mesoporous material, [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft], Confinement
Abstract: Aiming to rationalize the release profile of an incorporated pharmaceutical drug in terms of its mobility, driven by guest-host interactions, the poorly water-soluble Ibuprofen drug was loaded in a mesoporous inorganic silica matrix with unmodified (MCM-41) and modified surface (MCM-41sil) by post-synthesis silylation, both having pore sizes ∼ 3 nm. The single calorimetric detection of a broad glass transition step for both ibuprofen composites indicates full drug amorphization, confirmed by the only appearance of an amorphous halo in the powder XRD patterns. Moreover, a gradient profile is disclosed by the heat flux derivative plot in the glass transition, in coherence with the thermogravimetric profile that shows a multi-step decomposition trace for confined ibuprofen in these matrixes. While identical guest dynamics, as probed by dielectric relaxation spectroscopy, were found in both dehydrated composites, a significant molecular population with faster relaxation exists in the hydrated state for the drug inside the unmodified matrix. This was rationalized as the concurrence of true confinement effects, which manifest under nanometer dimensions, and greater water affinity of the unmodified matrix, forcing the drug molecules to be placed mostly in the pore core. Finite size effects are also felt in both dehydrated composites, however guest-host interactions give origin to a dominant population with slowed down mobility that governs the overall guest dynamics. In spite of an inferior number of active sites for drug adsorption in the silylated matrix, a faster Ibuprofen delivery in phosphate buffer (pH = 6.8) was observed when the drug is released from unmodified MCM-41 in the hydrated state. Therefore, our results suggest that a relevant role is played by water molecules, which impair a strong guest adsorption in the host surface more efficiently than the limited surface modification, influence the higher ratio of a faster population in the pore core and facilitate the diffusion of the aqueous releasing media inside pores.
Published: 2021
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37. Multidimensional ln-aminophthalate photoluminescent coordination polymers

Author: Queirós C., Sun C., Silva A.M.G., de Castro B., Cabanillas-Gonzalez J., Cunha-Silva L. and This research work received partial financial support from Portuguese national funds (FCT/MCTES, Funda??o para a Ci?ncia e Tecnologia and Minist?rio da Ci?ncia, Tecnologia e Ensino Superior) through the strategic project UIDB/50006/2020 (for LAQV-REQUIMTE), and it was also funded from the European Union (FEDER funds through COMPETE) and FCT/MCTES by National Funds to the R&D project X-Sensors (PTDC/QUI-QOR/29426/2017). IMDEA Nanociencia acknowledges support from the ?Severo Ochoa? Programme for Centres of Excellence in R&D (MINECO, grant SEV-2016-0686). J.C.-G. acknowledges financial support from the Spanish Ministry of Science, Innovation and Universities (RTI2018-097508-B-I00), (PCIN-2015-169-C02-01) and from the Regional Government of Madrid (NMAT2D S2018/NMT4511).
Published: 2021

38. A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants

Author: Yunho Lee, Manika Choudhury, Om Prakash, Donald Morrison, Hélène Guilloteau, Heidrun Mayrhofer, María Inmaculada Polo-López, Belen Esteban, Katarzyna Slipko, Leonardo Pantoja Munoz, Samira Nahim–Granados, José Manuel Guillén-Navarro, Marco Guida, Stela Krizanovic, Hemda Garelick, Pilar Caballero, Traore Ousmane, Marcos Quintela-Baluja, Sara Rodríguez-Mozaz, Helmut Bürgmann, Nikolina Udiković-Kolić, Carsten Ulrich Schwermer, Agnieszka Kalinowska, Milena Milaković, Ester Heath, Marta Piotrowska, Andrea Di Cesare, Célia M. Manaia, Ying Yang, Amy Pruden, Anne F.C. Leonard, Erica Donner, Tanel Telson, Christophe Merlin, Karin Beck, Ana Agüera, Anhony A Adegoke, Marie-Noëlle Pons, Bastian Herzog, Joana Abreu-Silva, Leonie Henn, Norbert Kreuzinger, Younggun Yoon, Stefanie Heß, Olga C. Nunes, Alice L. Petre, Despo Fatta-Kassinos, Gianluca Brunetti, Roberto B. M. Marano, Stella Michael, Thor A. Stenström, Ayella Maile-Moskowitz, Popi Karaolia, Joshua T. Bunce, Giovanni Libralato, Jérôme Ory, Yogesh Nimonkar, Alfieri Pollice, Aneta Luczkiewicz, Carlo Salerno, Andrew Scott, Shichun Zou, Antoni Oliver, Barbara Drigo, Isabel Martínez-Alcalá, Virginia Riquelme, Veljo Kisand, Anna Baraniak, William H. Gaze, Edward Topp, Edouard Jurkevitch, Isabel Henriques, Telma Fernandes, Eddie Cytryn, José Luis Balcázar, Marta Tacão, Thomas Schwartz, Thomas Jäger, Gianluca Corno, Mailis Laht, Thomas U. Berendonk, Roberto Rosal, Magdalena Popowska, Pawel Krzeminski, Connor L. Brown, Veritati - Repositório Institucional da Universidade Católica Portuguesa, Marano, R. B. M., Fernandes, T., Manaia, C. M., Nunes, O., Morrison, D., Berendonk, T. U., Kreuzinger, N., Telson, T., Corno, G., Fatta-Kassinos, D., Merlin, C., Topp, E., Jurkevitch, E., Henn, L., Scott, A., Hess, S., Slipko, K., Laht, M., Kisand, V., Di Cesare, A., Karaolia, P., Michael, S. G., Petre, A. L., Rosal, R., Pruden, A., Riquelme, V., Aguera, A., Esteban, B., Luczkiewicz, A., Kalinowska, A., Leonard, A., Gaze, W. H., Adegoke, A. A., Stenstrom, T. A., Pollice, A., Salerno, C., Schwermer, C. U., Krzeminski, P., Guilloteau, H., Donner, E., Drigo, B., Libralato, G., Guida, M., Burgmann, H., Beck, K., Garelick, H., Tacao, M., Henriques, I., Martinez-Alcala, I., Guillen-Navarro, J. M., Popowska, M., Piotrowska, M., Quintela-Baluja, M., Bunce, J. T., Polo-Lopez, M. I., Nahim-Granados, S., Pons, M. -N., Milakovic, M., Udikovic-Kolic, N., Ory, J., Ousmane, T., Caballero, P., Oliver, A., Rodriguez-Mozaz, S., Balcazar, J. L., Jager, T., Schwartz, T., Yang, Y., Zou, S., Lee, Y., Yoon, Y., Herzog, B., Mayrhofer, H., Prakash, O., Nimonkar, Y., Heath, E., Baraniak, A., Abreu-Silva, J., Choudhury, M., Munoz, L. P., Krizanovic, S., Brunetti, G., Maile-Moskowitz, A., Brown, C., Cytryn, E., The Robert H. Smith Faculty of Agriculture, Food and Environment, Department of Animal Sciences, The Hebrew University of Jerusalem (HUJ), Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization - the Volcani Center, Centro de Química Fina e Biotecnologia (CQFB), Requimte, Universidade do Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Universidade do Porto, Edinburgh Napier University, Institute for Hydrobiology, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Vienna University of Technology, Institute of Technology [Tartu, Estonia], University of Tartu, CNR Water Research Institute (IRSA), Consiglio Nazionale delle Ricerche (CNR), University of Cyprus (UCY), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Agriculture and Agri-Food [Ottawa] (AAFC), Department of Biology, University of Western Ontario, University of Western Ontario (UWO), Estonian Environmental Research Centre (EKUK), Universidad de Alcalá - University of Alcalá (UAH), Virginia Tech [Blacksburg], Area de Quimica Inorganica - Centro de Investigacion en Energia Solar (CIESOL) (CIESOL), Universidad de Almería (UAL), Gdańsk University of Technology (GUT), University of Exeter Medical School, University of Exeter, Durban University of Technology, Universidad Nacional de Cuyo [Mendoza] (UNCUYO), Norwegian Institute for Water Research (NIVA), University of South Australia [Adelaide], Università degli studi di Napoli Federico II, Swiss Federal Insitute of Aquatic Science and Technology [Dübendorf] (EAWAG), Middlesex University [London], Universidade de Aveiro, Universidade de Coimbra [Coimbra], Universidad Católica San Antonio de Murcia (UCAM), Department of Applied Microbiology [Warsaw], Institute of Microbiology [Warsaw], Faculty of Biology [Warsaw], University of Warsaw (UW)-University of Warsaw (UW)-Faculty of Biology [Warsaw], University of Warsaw (UW)-University of Warsaw (UW), School of Engineering [Newcastle], Newcastle University [Newcastle], Plataforma Solar de Almeria – CIEMAT, Tabernas, Almeria, Laboratoire Réactions et Génie des Procédés (LRGP), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Rudjer Boskovic Institute [Zagreb], Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), CHU Clermont-Ferrand, Laboratorio EMATSA, Instituto Catalán de Investigación del Agua - ICRA (SPAIN) (ICRA), Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), Sun Yat-Sen University [Guangzhou] (SYSU), Gwangju Institute of Science and Technology (GIST), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), NCMR, Jozef Stefan Institute [Ljubljana] (IJS), National Medicines Institute - Narodowy Instytut Leków [Warsaw] (NIL), Agricultural Research Organisation (ARO), Volcani Center, Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade do Porto = University of Porto, National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), University of Cyprus [Nicosia] (UCY), Agriculture and Agri-Food (AAFC), University of Naples Federico II = Università degli studi di Napoli Federico II, Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Marano, Robert BM, Fernandes, Telma, Manaia, Celia M, Nunes, Olga, Donner, Erica, Drigo, Barbara, Brunetti, Gianluca, and Cytryn, Eddie
Subjects: Cefotaxime, 010504 meteorology & atmospheric sciences, Coliforms, Antibiotic resistance, Sewage, Microorganismes -- Resistència als medicaments, Wastewater treatment, Wastewater, 010501 environmental sciences, 01 natural sciences, Surveys and Questionnaires, Microorganisms -- Drug resistance, ComputingMilieux_MISCELLANEOUS, lcsh:Environmental sciences, General Environmental Science, lcsh:GE1-350, 6. Clean water, Anti-Bacterial Agents, 3. Good health, Europe, Interdisciplinary Natural Sciences, Health, Sewage treatment, Biotechnology, medicine.drug, Life sciences, biology, Aigua -- Reutilització, Asia, Quantification methods, Aigües residuals -- Plantes de tractament, Culture and Communities, Water Purification, Applied microbiology, Water reuse, Environmental health, ddc:570, medicine, Microbiology Research Group, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, antibiotic resistance, coliforms, ESBLs, wastewater treatment, wastewater reuse, Sewage disposal plants, Effluent, 0105 earth and related environmental sciences, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, Coliform, business.industry, Australia, Fecal coliform, Antibiotic resistance, Coliforms, ESBLs, Wastewater treatment, Water reuse, ESBL, North America, Environmental science, business
Abstract: The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (
Published: 2020
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39. The Need to Develop Standard Measures of Patient Adherence for Big Data: Viewpoint

Author: Kardas, Przemyslaw, Aguilar-Palacio, Isabel, Almada, Marta, Cahir, Caitriona, Costa, Elisio, Giardini, Anna, Malo, Sara, Massot Mesquida, Mireia, Menditto, Enrica, Midão, Luís, Parra-Calderón, Carlos Luis, Pepiol Salom, Enrique, Vrijens, Bernard, [Kardas,P] Department of Family Medicine, Medical University of Lodz, Lodz, Poland. [Aguilar-Palacio,I, Malo,S] Preventive Medicine and Public Health Department, Zaragoza University, Zaragoza, Spain. [Aguilar-Palacio,I, Malo,S] Fundación Instituto de Investigación Sanitaria de Aragón (IIS Aragón), Zaragoza, Spain. [Almada,M, Costa,E, and Midão,L] UCIBIO REQUIMTE, ICBAS, Porto4Ageing - Competences Center on Active and Healthy Ageing, Faculty of Pharmacy, University of Porto, Porto, Portugal. [Cahir,C] Division of Population Health Sciences, Royal College of Surgeons in Ireland, Dublin, Ireland. [Giardini,A] IT Department, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, Italy. [Massot Mesquida,M] Servei d’Atenció Primària Vallès Occidental, Institut Català de la Salut, Barcelona, Spain. [Menditto,E] CIRFF, Center of Pharmacoeconomics, University of Naples Federico II, Naples, Italy. [Menditto,E] Department of Pharmacy, University of Naples Federico II, Naples, Italy. [Parra-Calderón,CE] Group of Research and Innovation in Biomedical Informatics, Biomedical Engineering and Health Economy, Institute of Biomedicine of Seville, IBiS / Virgen del Rocío University Hospital / CSIC / University of Seville, Seville, Spain. [Pepiol Salom,E] International Commitee, Muy Ilustre Colegio Oficial de Farmacéuticos, Valencia, Spain. [Vrijens,B] AARDEX Group, Seraing, Belgium. [Vrijens,B] Liège University, Liège, Belgium.
Subjects: Consensus, Sistemas informatizados de historias clínicas, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Case-Control Studies::Retrospective Studies [Medical Subject Headings], Psychiatry and Psychology::Behavior and Behavior Mechanisms::Psychology, Social::Group Processes::Consensus [Medical Subject Headings], Electronic health record, Information Science::Information Science::Medical Informatics::Medical Informatics Applications::Information Systems::Medical Records Systems, Computerized [Medical Subject Headings], Patient adherence, Health care, Adhesión a la medicación, Macrodatos, Registros electrónicos de salud, Health Care::Health Care Quality, Access, and Evaluation::Delivery of Health Care::Attitude to Health::Patient Acceptance of Health Care::Patient Compliance::Medication Adherence [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Big data, Benchmarking, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Records as Topic::Medical Records::Medical Records Systems, Computerized::Electronic Health Records [Medical Subject Headings], Consenso, Phenomena and Processes::Physiological Phenomena::Physiological Processes::Growth and Development::Aging [Medical Subject Headings], Metrics, Cooperación del paciente, Health Care::Health Care Quality, Access, and Evaluation::Quality of Health Care::Health Care Evaluation Mechanisms::Program Evaluation::Benchmarking [Medical Subject Headings], Medication adherence, Atención a la salud, Psychiatry and Psychology::Behavior and Behavior Mechanisms::Behavior::Health Behavior::Patient Compliance [Medical Subject Headings]
Abstract: Despite half a century of dedicated studies, medication adherence remains far from perfect, with many patients not taking their medications as prescribed. The magnitude of this problem is rising, jeopardizing the effectiveness of evidence-based therapies. An important reason for this is the unprecedented demographic change at the beginning of the 21st century. Aging leads to multimorbidity and complex therapeutic regimens that create a fertile ground for nonadherence. As this scenario is a global problem, it needs a worldwide answer. Could this answer be provided, given the new opportunities created by the digitization of health care? Daily, health-related information is being collected in electronic health records, pharmacy dispensing databases, health insurance systems, and national health system records. These big data repositories offer a unique chance to study adherence both retrospectively and prospectively at the population level, as well as its related factors. In order to make full use of this opportunity, there is a need to develop standardized measures of adherence, which can be applied globally to big data and will inform scientific research, clinical practice, and public health. These standardized measures may also enable a better understanding of the relationship between adherence and clinical outcomes, and allow for fair benchmarking of the effectiveness and cost-effectiveness of adherence-targeting interventions. Unfortunately, despite this obvious need, such standards are still lacking. Therefore, the aim of this paper is to call for a consensus on global standards for measuring adherence with big data. More specifically, sound standards of formatting and analyzing big data are needed in order to assess, uniformly present, and compare patterns of medication adherence across studies. Wide use of these standards may improve adherence and make health care systems more effective and sustainable. Yes
Published: 2020

40. Next-generation care pathways for allergic rhinitis and asthma multimorbidity: a model for multimorbid non-communicable diseases -Meeting Report (Part 1)

Author: Elaine Colgan, Mario Sánchez-Borges, Jim Phillips, Ignacio J. Ansotegui, Bolesław Samoliński, Olga Lourenço, Giorgio Walter Canonica, F. Portejoie, Isabella Annesi-Maesano, Gabrielle L. Onorato, Victoria Cardona, João O. Malva, Eugene Cash, Christine Rolland, Hilary Pinnock, Samantha Walker, Yoshitaka Okamoto, Ana Maria Carriazo, Lorenzo Cecchi, Nikos G. Papadopoulos, Jean Bousquet, L. T. T. Le, João Fonseca, Oliver Pfaar, Luis Caraballo, Elísio Costa, Maritta Perala, Pablo Quinones-Delgado, Torsten Zuberbier, Holger J. Schünemann, Tomohisa Iinuma, Enrica Menditto, Maddalena Illario, Nils E. Billo, Arunas Valiulis, Sinthia Bosnic-Anticevich, Josep M. Antó, Nick A. Guldemond, Maria Teresa Ventura, Claus Bachert, Wytske Fokkens, Lars Münter, Mohamed H. Shamji, Ioana Agache, Ulysse Rodts, Daniel Laune, Sanna Toppila-Salmi, Joaquim Mullol, Ioanna Tsiligianni, Motohiro Ebisawa, Isabelle Bosse, Samuel Benveniste, Arzu Yorgancioglu, Alkis Togias, M. Bewick, Nhân Pham-Thi, Stephen R. Durham, Moises A. Calderon, Marina Erhola, Violeta Kvedariene, Omar S. Usmani, Alvaro A. Cruz, Anna Bedbrook, Abigail Phillips, Desiree Larenas-Linneman, Guy Brusselle, Gert Marien, Dana Wallace, David Somekh, Sian Williams, Wienczyslawa Czarlewski, Ludger Klimek, Piotr Kuna, Jean-Luc Fauquert, Rianne van der Kleij, Derek K. Chu, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Contre les MAladies Chroniques pour un VIeillissement Actif en Languedoc-Roussillon (MACVIA-LR), Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Nîmes (CHU Nîmes)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-European Innovation Partnership on Active and Healthy Ageing Reference Site (EIP on AHA), Commission Européenne-Commission Européenne-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Service de Pneumologie Allergologie [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris], Transilvania University of Brasov, Epidémiologie des maladies infectieuses et modélisation (ESIM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospital Quiròn Bizkaia Erandio, Universitat Pompeu Fabra [Barcelona] (UPF), CIBER de Epidemiología y Salud Pública (CIBERESP), Ghent University Hospital, Centre de Recherche en Informatique (CRI), MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), AP-HP - Hôpital Cochin Broca Hôtel Dieu [Paris], iQ4U consultants Ltd, University of Joensuu, The University of Sydney, Imperial College London, Humanitas Clinical and Research Center [Rozzano, Milan, Italy], Institute for Immunological Research (University of Cartagena), Vall d'Hebron University Hospital [Barcelona], Servicio Andaluz de Salud, Nova Southeastern University (NSU), Azienda Sanitaria di Prato, McMaster University [Hamilton, Ontario], DEPARTMENT OF HEALTH ( Social Services and Public Safety), Universidade do Porto, Universidade Federal da Bahia (UFBA), UCB Pharma, Colombes, Sagamihara National Hospital, National Institute for Health and Welfare [Helsinki], CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand, European Forum for Research and Education in Allergy and Airway Diseases (EUFOREA), Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA), Faculdade de Medicina da Universidade do Porto (FMUP), Erasmus University Medical Center [Rotterdam] (Erasmus MC), Chiba University Hospital, 'Federico II' University of Naples Medical School, Zentrum für Rhinologie und Allergologie [Wiesbaden, Germany], Medical University of Łódź (MUL), Vilnius University [Vilnius], Hospital Medica Sur [Mexico City, Mexico], KYomed INNOV, Ho Chi Minh City University of Technology (HCMUT), Faculty of Health Sciences and CICS-UB (Health Sciences Research Centre), University of Coimbra [Portugal] (UC), Centro de Investigación Biomédica en Red Enfermedades Respiratorias (CIBERES), Department of Public Health [Copenhagen], Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), University of Manchester [Manchester], University of Athens Medical School [Athens], University of Oulu, Philipps University of Marburg, Cardiff University, University of Edinburgh, Regional Government for Equality, Medical University of Warsaw - Poland, Centro Medico-Docente La Trinidad, Department of Clinical Epidemiology and Biostatistics and Medicine, National Institute of Allergy and Infectious Diseases [Bethesda] (NIAID-NIH), National Institutes of Health [Bethesda] (NIH), University of Helsinki, University of Crete [Heraklion] (UOC), National Heart and Lung Institute [London] (NHLI), Imperial College London-Royal Brompton and Harefield NHS Foundation Trust, King's College Hospital (KCH), Leiden University Medical Center (LUMC), University of Bari Aldo Moro (UNIBA), Manisa Celal Bayar University, Humboldt-Universität zu Berlin, Association Asthme et Allergie, Health Services Management & Organisation (HSMO), University Hospital Montpellier, Montpellier, France, MACVIA-France, Fondation partenariale FMC VIA-LR, Montpellier, France, INSERM U 1168, VIMA, Ageing and Chronic Diseases Epidemiological and Public Health Approaches, Villejuif, France, Université Versailles St-Quentin-en-Yvelines, UMR-S 1168, Montigny le Bretonneux, France, EUFOREA, Brussels, Belgium, Charité, Universitätsmedizin Berlin, Humboldt-Universität zu Berlin, Berlin, Germany, Berlin Institute of Health, Comprehensive Allergy Center, Department of Dermatology and Allergy, Berlin, Germany, Allergy Department, Pasteur Institute, Paris, France, Faculty of Medicine, Transylvania University, Brasov, Romania, Epidemiology of Allergic and Respiratory Diseases, Department Institute Pierre Louis of Epidemiology and Public Health, INSERM, Sorbonne Universités, Medical School Saint Antoine, Paris, France, Department of Allergy and Immunology, Hospital Quirónsalud Bizkaia, Erandio, Spain, ISGlobAL, Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, CIBER Epidemiolog a y Salud Pública (CIBERESP), Barcelona, Spain, Upper Airways Research Laboratory, ENT Dept., Ghent University Hospital, Ghent, Belgium, National Center of Expertise in Cognitive Stimulation (CEN STIMCO), Broca Hospital, Paris, France, Mines ParisTech CRI - PSL Research University, Fontainebleau, France, iQ4U Consultants Ltd., London, United Kingdom, Joensuu, Finland, Woolcock Institute of Medical Research, University of Sydney, Woolcock Emphysema Centre, Sydney Local Health District, Glebe, NSW, Australia, La Rochelle, France, Dept. of Respiratory Medicine, Ghent University Hospital, Ghent, Belgium, Imperial College London, National Heart and Lung Institute, London, United Kingdom, Personalized Medicine Clinic Asthma and Allergy, Department of Biomedical Sciences, Humanitas University, Pieve Emanuele (MI), Italy, Institute for Immunological Research, University of Cartagena, Campus de Zaragocilla, Edificio Biblioteca Primer piso, Cartagena, Colombia, Foundation for the Development of Medical and Biological Sciences (Fundemeb), Cartagena, Colombia, Allergy Section, Department of Internal Medicine, Hospital Vall d’Hebron and ARADyAL Research Network, Barcelona, Spain, Regional Ministry of Health of Andalusia, Seville, Spain, College of Psychology, Nova Southeastern University, School-related Psychological Assessments and Clinical Interventions Clinic, Ft Lauderdale, FL, United States, SOS Allergology and Clinical Immunology, USL Toscana Centro, Prato, Italy, Department of Health Research Methods, Evidence, and Impact, Division of Immunology and Allergy, Department of Medicine, McMaster University, Hamilton, ON, Canada, Department of Health, Social Services and Public Safety, Belfast, United Kingdom, UCIBIO, REQUIMTE, Faculty of Pharmacy, Competence Center on Active and Healthy Ageing of University of Porto (AgeUPNetWork), University of Porto, Porto, Portugal, ProAR-Nucleo de Excelencia em Asma, Federal University of Bahia, Brasil and WHO GARD Executive Committee, Bahia, Brazil, Medical Consulting Czarlewski, Levallois, France, Allergy and Clinical Immunology Section, National Heart and Lung Institute, Imperial College London, London, United Kingdom, Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Japan, National Institute for Health and Welfare, Helsinki, Finland, CHU Clermont-Ferrand, Unité d’Allergologie de l’Enfant, Pôle Pédiatrique, Hôpital Estaing, Clermont-Ferrand, France, Department of Otorhinolaryngology, Amsterdam University Medical Centres, AMC, Amsterdam, Netherlands, CINTESIS, Center for Research in Health Technology and Information Systems, Faculdade de Medicina, Universidade do Porto, Medida, Lda, Porto, Portugal, Institute of Health Policy and Management iBMG, Erasmus University, Rotterdam, Netherlands, Dept. of Otorhinolaryngology, Chiba University Hospital, Chiba, Japan, Division for Health Innovation, Campania Region and Federico II University Hospital Naples (R and D and DISMET), Naples, Italy, Center for Rhinology and Allergology, Wiesbaden, Germany, Division of Internal Medicine, Asthma and Allergy, Barlicki University Hospital, Medical University of Lodz, Lodz, Poland, Institute of Biomedical Sciences, Department of Pathology, Faculty of Medicine, Vilnius University, Vilnius, Lithuania, Institute of Clinical Medicine, Clinic of Chest Diseases and Allergology, Faculty of Medicine, Vilnius, Lithuania, Center of Excellence in Asthma and Allergy, Médica Sur Clinical Foundation and Hospital, México City, Mexico, KYomed INNOV, Montpellier, France, University of Medicine and Pharmacy, Hochiminh City, Viet Nam, Faculty of Health Sciences, CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal, Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Ageing@Coimbra EIP-AHA Reference Site, Coimbra, Portugal, CIRFF, Center of Pharmacoeconomics, University of Naples Federico II, Naples, Italy, Rhinology Unit and Smell Clinic, ENT Department, Hospital Cl nic, Clinical and Experimental Respiratory Immunoallergy, IDIBAPS, CIBERES, University of Barcelona, Barcelona, Spain, Danish Committee for Health Education, Copenhagen East, Denmark, Division of Infection, Immunity and Respiratory Medicine, Royal Manchester Children’s Hospital, University of Manchester, Manchester, United Kingdom, Allergy Department, 2nd Pediatric Clinic, Athens General Children’s Hospital 'P and A Kyriakou', University of Athens, Athens, Greece, University of Oulu, Faculty of Medicine, Oulun Yliopisto, Finland, Department of Otorhinolaryngology, Head and Neck Surgery, Section of Rhinology and Allergy, University Hospital Marburg, Phillipps-Universität Marburg, Germany, Department of Health and Social Services, Welsh Government, Cardiff, United Kingdom, Centre For Empowering Patients and Communities, Dublin, Ireland, Asthma UK Centre for Applied Research, Usher Institute of Population Health Sciences and Informatics, University of Edinburgh, Edinburgh, United Kingdom, Agency for Social Services and Dependency, Regional Government for Equality, Social Policies and Conciliation of Andalucia, Seville, Spain, Association Asthme et Allergie, Paris, France, KanopyMed, Paris, France, Department of Prevention of Environmental Hazards and Allergology, Medical University of Warsaw, Warsaw, Poland, Allergy and Clinical Immunology Department, Centro Medico-Docente La Trinidad, Caracas, Venezuela, Immunomodulation and Tolerance Group, Imperial College London, London, United Kingdom, Allergy and Clinical Immunology, Imperial College London, London, United Kingdom, European Health Futures Forum (EHFF), Dromahair, Ireland, Division of Allergy, Immunology, and Transplantation (DAIT), National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD, United States, Skin and Allergy Hospital, Helsinki University Hospital, University of Helsinki, Helsinki, Finland, Health Planning Unit, Department of Social Medicine, Faculty of Medicine, University of Crete, Crete, Greece, International Primary Care Respiratory Group IPCRG, Aberdeen, United Kingdom, National Heart and Lung Institute (NHLI), Imperial College London, Royal Brompton Hospital, Airways Disease Section, London, United Kingdom, Asthma UK, London, United Kingdom, Nova Southeastern University, Fort Lauderdale, FL, United States, Vilnius University, Faculty of Medicine, Institute of Clinical Medicine, Institute of Health Sciences, Vilnius, Lithuania, Department of Public Health and Primary Care, Leiden University Medical Center, Leiden, Netherlands, University of Bari Medical School, Unit of Geriatric Immunoallergology, Bari, Italy, Department of Pulmonary Diseases, Celal Bayar University, Faculty of Medicine, Manisa, Turkey, Universitätsmedizin Berlin, Humboldt-Uniersität zu Berlin, Berlin, Germany, Berlin Institute of Health, Comprehensive Allergy-Centre, Department of Dermatology and Allergy, Berlin, Germany, uBibliorum, Ear, Nose and Throat, AII - Inflammatory diseases, Institut Pasteur [Paris] (IP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Hospital Quirónsalud Bizkaia [Bilbao], Mines Paris - PSL (École nationale supérieure des mines de Paris), Universidade do Porto = University of Porto, Sagamihara National Hospital [Kanagawa, Japan], University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH), Philipps Universität Marburg = Philipps University of Marburg, Centro Médico Docente La Trinidad, Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), Humboldt University Of Berlin, Salvy-Córdoba, Nathalie, Universiteit Leiden, Bousquet, J., Pham-Thi, N., Bedbrook, A., Agache, I., Annesi-Maesano, I., Ansotegui, I., Anto, J. M., Bachert, C., Benveniste, S., Bewick, M., Billo, N., Bosnic-Anticevich, S., Bosse, I., Brusselle, G., Calderon, M. A., Canonica, G. W., Caraballo, L., Cardona, V., Carriazo, A. M., Cash, E., Cecchi, L., Chu, D. K., Colgan, E., Costa, E., Cruz, A. A., Czarlewski, W., Durham, S., Ebisawa, M., Erhola, M., Fauquert, J. -L., Fokkens, W. J., Fonseca, J. A., Guldemond, N., Iinuma, T., Illario, M., Klimek, L., Kuna, P., Kvedariene, V., Larenas-Linneman, D., Laune, D., Le, L. T. T., Lourenco, O., Malva, J. O., Marien, G., Menditto, E., Mullol, J., Munter, L., Okamoto, Y., Onorato, G. L., Papadopoulos, N. G., Perala, M., Pfaar, O., Phillips, A., Phillips, J., Pinnock, H., Portejoie, F., Quinones-Delgado, P., Rolland, C., Rodts, U., Samolinski, B., Sanchez-Borges, M., Schunemann, H. J., Shamji, M., Somekh, D., Togias, A., Toppila-Salmi, S., Tsiligianni, I., Usmani, O., Walker, S., Wallace, D., Valiulis, A., Van Der Kleij, R., Ventura, M. T., Williams, S., Yorgancioglu, A., and Zuberbier, T.
Subjects: Pulmonary and Respiratory Medicine, Allergy, medicine.medical_specialty, Allergic and chronic respiratory diseases, [SDV.IMM] Life Sciences [q-bio]/Immunology, Allergic rhinitis - Asthma - Multimorbidity, Health care system, Health literacy, 03 medical and health sciences, 0302 clinical medicine, SDG 3 - Good Health and Well-being, Health care, medicine, Patient participation, mHealth, Asthma, Rhinitis, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Newsletter of GARD Section, business.industry, Environmental exposure, medicine.disease, 3. Good health, Integrated care, [SDV.EE] Life Sciences [q-bio]/Ecology, environment, 030228 respiratory system, 13. Climate action, Family medicine, [SDV.IMM]Life Sciences [q-bio]/Immunology, 030211 gastroenterology & hepatology, business, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; In all societies, the burden and cost of allergic and chronic respiratory diseases are increasing rapidly. Most economies are struggling to deliver modern health care effectively. There is a need to support the transformation of the health care system for integrated care with organizational health literacy. MASK (Mobile Airways Sentinel NetworK) (1), a new development of the ARIA (Allergic Rhinitis and its Impact on Asthma) initiative, and POLLAR (Impact of Air POLLution on Asthma and Rhinitis, EIT Health) (2), in collaboration with professional and patient organizations in the field of allergy and airway diseases, are proposing real-life integrated care pathways (ICPs) (3)-centred around the patient with rhinitis and using mHealth monitoring of environmental exposure (4).An expert meeting took place at the Pasteur Institute in Paris, December 3, 2018. The aim was to discuss next-generation care pathways: (I) Patient participation, health literacy and self-care through technology-assisted “patient activation”; (II) Implementation of care pathways by pharmacists and (III) Next-generation guidelines assessing the recommendations of GRADE guidelines in rhinitis and asthma using real-world evidence (RWE) assessed by mobile technology.The EU (5) and global political agendas are of great importance in supporting health care transformation. MASK has been recognized by DG Santé as a Good Practice (6) in the field of digitally-enabled, integrated, person-centred care.The one-day meeting objectives were clear (Figure 1). The meeting was followed by a workshop. The present paper reports the background of the two-day meeting.
Published: 2019
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41. Next-Generation Sequencing and MALDI Mass Spectrometry in the Study of Multiresistant Processed Meat Vancomycin-Resistant Enterococci (VRE)

Author: Gilberto Igrejas, Richard Bonnet, Christophe Chambon, Michel Hébraud, Patrícia Poeta, Racha Beyrouthy, Laetitia Théron, Didier Viala, Telma de Sousa, Soraia Oliveira, Manuela Caniça, Carolina Sabença, University of Trás-os-Montes and Alto Douro [Portugal] (UTAD), Laboratorio Associado Para a Quimica Verde (LAQV/REQUIMTE), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Exploration du Métabolisme (PFEM), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-MetaboHUB-Clermont, MetaboHUB-MetaboHUB, Microbiologie Environnement Digestif Santé (MEDIS), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de Référence de la Résistance aux Antibiotiques (CNR), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre de Recherche en Nutrition Humaine d'Auvergne (CRNH d'Auvergne)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, Instituto Nacional de Saùde Dr Ricardo Jorge [Portugal] (INSA), Faculty of Science Oporto University, Centre for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, Oporto University, 4051-401 Oporto, 'Espectrometria de massa e sequenciacao aplicadas a microbiologia clinica moderna: pesquisa e identificacao de biomarcadores em especies bacterianas multiresistentes' (Programa de Accoes Universitarias Integradas Luso-Francesas) TC-14/2017Associate Laboratory for Green Chemistry, LAQV - FCT/MCTES UID/QUI/50006/2020CT/MCTES UIDB/00211/2020, LAQV@REQUIMTE, Plateforme Exploration du Métabolisme-MetaboHUB (PFEM-MetaboHUB), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), ROSSI, Sabine, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and INRAE, Plateforme d’Exploration du Métabolisme, Composante Protéomique (PFEMcp), 63122 Saint-Genès Champanelle
Subjects: 0301 basic medicine, antibiotic resistance, Tetracycline, Antibiotic resistance, [SDV]Life Sciences [q-bio], 030106 microbiology, Processed meat, General Biochemistry, Genetics and Molecular Biology, Article, Microbiology, 03 medical and health sciences, Immunology and Microbiology(all), medicine, MALDI-TOF MS, lcsh:QH301-705.5, Enterococcus spp, processed meat, General Immunology and Microbiology, biology, Agricultural and Biological Sciences(all), Resistência aos Antimicrobianos, Teicoplanin, Biochemistry, Genetics and Molecular Biology(all), Kanamycin, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, 3. Good health, [SDV] Life Sciences [q-bio], 030104 developmental biology, Enterococcus, lcsh:Biology (General), Streptomycin, Next-generation sequencing, Gentamicin, next-generation sequencing, General Agricultural and Biological Sciences, medicine.drug, Enterococcus faecium
Abstract: Vancomycin-resistant enterococci (VRE), due to their intrinsic resistance to various commonly used antibiotics and their malleable genome, make the treatment of infections caused by these bacteria less effective. The aims of this work were to characterize isolates of Enterococcus spp. that originated from processed meat, through phenotypic and genotypic techniques, as well as to detect putative antibiotic resistance biomarkers. The 19 VRE identified had high resistance to teicoplanin (89%), tetracycline (94%), and erythromycin (84%) and a low resistance to kanamycin (11%), gentamicin (11%), and streptomycin (5%). Based on a Next-Generation Sequencing NGS technique, most isolates were vanA-positive. The most prevalent resistance genes detected were erm(B) and aac(6')-Ii, conferring resistance to the classes of macrolides and aminoglycosides, respectively. MALDI-TOF mass spectrometry (MS) analysis detected an exclusive peak of the Enterococcus genus at m/z (mass-to-charge-ratio) 4428 &plusmn, 3, and a peak at m/z 6048 &plusmn, 1 allowed us to distinguish Enterococcus faecium from the other species. Several statistically significant protein masses associated with resistance were detected, such as peaks at m/z 6358.27 and m/z 13237.3 in ciprofloxacin resistance isolates. These results reinforce the relevance of the combined and complementary NGS and MALDI-TOF MS techniques for bacterial characterization.
Published: 2020
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42. The importance of wheat

Author: Gilberto Igrejas, Gérard Branlard, Department of Genetics and Biotechnology, Universidade de Trás-os-Montes e Alto Douro, Functional Genomics and Proteomics Unity, University of Tras-os-Montes and Alto Douro, LAQV-REQUIMTE, Universidade NOVA de Lisboa, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Gilberto Igrejas, Tatsuya M. Ikeda, Carlos Guzmán, and Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)
Subjects: media_common.quotation_subject, [SDV]Life Sciences [q-bio], Industrial uses, Wheat dissemination, 03 medical and health sciences, Some questions, 0404 agricultural biotechnology, Food supply, Quality (business), Domestication, 030304 developmental biology, media_common, 2. Zero hunger, chemistry.chemical_classification, 0303 health sciences, business.industry, food and beverages, 04 agricultural and veterinary sciences, 040401 food science, Gluten, Biotechnology, chemistry, Food processing, Grain yield, Urban life, business, Essential nutrient, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, World production
Abstract: The history of wheat domestication and use is closely linked to the efforts of humans to protect themselves from hunger and gain control over their food supply. Now grown worldwide wheat has become the most important source of food. For centuries bread wheat (Triticum aestivum) and durum wheat (Triticum durum) have been cultivated in the West to provide humans with energy and essential nutrients. Today China and India are the top two wheat-producing countries, largely because wheat has the advantage of requiring less water for cultivation than other comparable crops while being the main ingredient of a variety of processed foods valued in modern, mainly urban life. For more than a century, breeders have continuously improved wheat focusing on factors affecting grain yield and, more recently, technological quality. The properties of wheat that are ideal for processing into different food products have been greatly improved since the 1960s thanks to detailed research on storage proteins, which constitute the gluten. Most of these genetic successes are referred to in this book but many important goals remain to be achieved. Today further progress is crucial in the use of shared genetic resources, common analytical protocols for allele identification and technological processing, and dedicated tools for analysing polymer formation and characterisation particularly in response to climatic and other environmental factors. Technological properties are not the only wheat quality attributes, as consumers are increasingly aware and concerned about the nutritional value (the content in fiber, minerals, macro- and micro-nutrients, vitamins) and the health impact, whether positive or negative. For example, research on several pathologies associated with the consumption of gluten-based products will require collaboration between allergy specialists and wheat protein geneticists.
Published: 2020
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43. NO Releasing and Anticancer Properties of Octahedral Ruthenium–Nitrosyl Complexes with Equatorial 1H-Indazole Ligands

Author: Vladimir B. Arion, Wee Han Ang, Ewelina Orlowska, Tara E. McDonnell, Maria V. Babak, Peter Rapta, Chinju Govind, Dominik Schaniel, Orsolya Dömötör, Venugopal Karunakaran, Michal Malček, Dominique Luneau, Éva A. Enyedy, Lukáš Bučinský, Yusuf Chouthury Shaik Farid, Michal Zalibera, Universität Wien, National University of Singapore (NUS), University of Szeged [Szeged], Slovak University of Technology in Bratislava, REQUIMTE/LAQV, Faculdade de Ciências, Universidade do Porto, National Institute for Interdisciplinary Science and Technology (CSIR), National Institute for Interdisciplinary Science and Technology [India], Nanyang Polytechnic - NYP (REPUBLIC OF SINGAPORE), School of Biotechnology and Biomolecular Sciences, University of New South Wales [Sydney] (UNSW), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Cristallographie, Résonance Magnétique et Modélisations (CRM2), and Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Models, Molecular, Indazoles, Cell Survival, Blotting, Western, chemistry.chemical_element, Antineoplastic Agents, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, Ligands, Nitric Oxide, 010402 general chemistry, 01 natural sciences, Medicinal chemistry, Ruthenium, Inorganic Chemistry, Inhibitory Concentration 50, chemistry.chemical_compound, Deprotonation, Drug Stability, X-Ray Diffraction, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Electrochemistry, Organometallic Compounds, Acetone, Humans, Physical and Theoretical Chemistry, Dichloromethane, Indazole, 010405 organic chemistry, Water, HCT116 Cells, 0104 chemical sciences, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, chemistry, Octahedron, Proton NMR, Quantum Theory, Nitrogen Oxides, Cisplatin, Cyclic voltammetry
Abstract: International audience; With the aim of enhancing the biological activity of ruthenium−nitrosyl complexes, new compounds with four equatorially bound indazole ligands, namely, trans-[RuCl(Hind) 4 (NO)]Cl 2 •H 2 O ([3]Cl 2 •H 2 O) and trans-[RuOH(Hind) 4 (NO)]Cl 2 •H 2 O ([4]Cl 2 •H 2 O), have been prepared from trans-[Ru(NO 2) 2 (Hind) 4 ] ([2]). When the pH-dependent solution behavior of [3]Cl 2 •H 2 O and [4]Cl 2 • H 2 O was studied, two new complexes with two deprotonated indazole ligands were isolated, namely [RuCl(ind) 2 (Hind) 2 (NO)] ([5]) and [RuOH(ind) 2 (Hind) 2 (NO)] ([6]). All prepared compounds were comprehensively characterized by spectroscopic (IR, UV−vis, 1 H NMR) techniques. Compound [2], as well as [3]Cl 2 •2(CH 3) 2 CO, [4]Cl 2 •2(CH 3) 2 CO, and [5]•0.8CH 2 Cl 2 , the latter three obtained by recrystallization of the first isolated compounds (hydrates or anhydrous species) from acetone and dichloromethane, respectively, were studied by X-ray diffraction methods. The photoinduced release of NO in [3]Cl 2 and [4]Cl 2 was investigated by cyclic voltammetry and resulting paramagnetic NO species were detected by EPR spectroscopy. The quantum yields of NO release were calculated and found to be low (3−6%), which could be explained by NO dissociation and recombination dynamics, assessed by femtosecond pump−probe spectroscopy. The geometry and electronic parameters of Ru species formed upon NO release were identified by DFT calculations. The complexes [3]Cl 2 and [4]Cl 2 showed considerable antiproliferative activity in human cancer cell lines with IC 50 values in low micromolar or submicromolar concentration range and are suitable for further development as potential anticancer drugs. p53-dependence of Ru−NO complexes [3]Cl 2 and [4]Cl 2 was studied and p53-independent mode of action was confirmed. The effects of NO release on the cytotoxicity of the complexes with or without light irradiation were investigated using NO scavenger carboxy-PTIO.
Published: 2018
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44. Opioids and the Blood-Brain Barrier: A Dynamic Interaction with Consequences on Drug Disposition in Brain

Author: Salvatore Cisternino, Xavier Declèves, Catarina Chaves, Fernando Remião, Variabilité de réponse aux Psychotropes (VariaPsy - U1144), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Requimte, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), and Decleves, Xavier
Subjects: 0301 basic medicine, opioids, Abcg2, [SDV]Life Sciences [q-bio], ATP-binding cassette transporter, Pharmacology, P-glycoprotein, Article, neuroinflammation, 03 medical and health sciences, 0302 clinical medicine, Medicine, Animals, Humans, Pharmacology (medical), TLR4, Neuroinflammation, biology, business.industry, Brain, Biological Transport, General Medicine, 3. Good health, Solute carrier family, [SDV] Life Sciences [q-bio], Analgesics, Opioid, Psychiatry and Mental health, 030104 developmental biology, Neurology, Opioid, ABC transporters, Nonlinear Dynamics, Blood-Brain Barrier, biology.protein, ATP-Binding Cassette Transporters, Neurology (clinical), Efflux, business, 030217 neurology & neurosurgery, medicine.drug
Abstract: International audience; Background: Opioids are widely used in pain management, acting via opioid receptors and/or Toll-like receptors (TLR) present at the central nervous system (CNS). At the blood-brain barrier (BBB), several influx and efflux transporters, such as the ATP-binding cassette (ABC) P-glycoprotein (P-gp, ABCB1), Breast Cancer Resistance Protein (BCRP, ABCG2) and multidrug resistance-associated proteins (MRP, ABCC) transporters, and solute carrier transporters (SLC), are responsible for the transport of xenobiotics from the brain into the bloodstream or vice versa.Objective: ABC transporters export several clinically employed opioids, altering their neuropharmacokinetics and CNS effects. In this review, we explore the interactions between opioids and ABC transporters, and decipher the molecular mechanisms by which opioids can modify their expression at the BBB.Results: P-gp is largely implicated in the brain-to-blood efflux of opioids, namely morphine and oxycodone. Long-term exposure to morphine and oxycodone has proven to up-regulate the expression of ABC transporters, such as P-gp, BCRP and MRPs, at the BBB, which may lead to increased tolerance to the antinociceptive effects of such drugs. Recent studies uncover two mechanisms by which morphine may up-regulate P-gp and BCRP at the BBB: 1) via a glutamate, NMDA-receptor and COX-2 signaling cascade, and 2) via TLR4 activation, subsequent development of neuroinflammation, and activation of NF-kB, presumably via glial cells.Conclusion: The BBB-opioid interaction can culminate in bilateral consequences, since ABC transporters condition the brain disposition of opioids, while opioids also affect the expression of ABC transporters at the BBB, which may result in increased CNS drug pharmacoresistance.
Published: 2017
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45. Inter-laboratory calibration of quantitative analyses of antibiotic resistance genes

Author: Popi Karaolia, Jaqueline Rocha, Thomas U. Berendonk, Célia M. Manaia, Despo Fatta-Kassinos, Ioannis D. Kampouris, Thomas Schwartz, Hélène Guilloteau, Eddie Cytryn, Christophe Merlin, Roberto B. M. Marano, Damiano Cacace, Thomas Jäger, Centro de Química Fina e Biotecnologia (CQFB), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, Institut für Hydrobiologie, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization - the Volcani Center, University of Cyprus (UCY), and Veritati - Repositório Institucional da Universidade Católica Portuguesa
Subjects: Life sciences, biology, 0301 basic medicine, Calibration (statistics), Inter-laboratory calibration, Computational biology, 010501 environmental sciences, Biology, Wastewater, Antibiotic resistance gene, 01 natural sciences, 03 medical and health sciences, Quantitative PCR, Plasmid, Antibiotic resistance, ddc:570, Chemical Engineering (miscellaneous), Inter-laboratory, Waste Management and Disposal, Gene, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, Process Chemistry and Technology, 16S ribosomal RNA, Pollution, 6. Clean water, 030104 developmental biology, Real-time polymerase chain reaction, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Antibiotic resistance genes
Abstract: Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are widely distributed in the environment where they represent potential public health threats. Quantitative PCR (qPCR) is a suitable approach to detect and quantify ARGs in environmental samples. However, the comparison of gene quantification data between different laboratories is challenging since the data are predominantly obtained under non-harmonized conditions, using different qPCR protocols. This study aimed at carrying out an inter-laboratory calibration in order to assess the variability inherent to the qPCR procedures for quantification of ARGs. With this aim, samples of treated wastewater collected in three different countries were analysed based on common DNA extract pools and identical protocols as well as distinct equipment, reagents batches, and operators. The genes analysed were the 16S rRNA, vanA, blaTEM, qnrS, sul1, blaCTXM-32 and intI1 and the artificial pNORM1 plasmid containing fragments from the seven targeted genes was used as a reference. The 16S rRNA gene was the most abundant, in all the analysed samples, followed by intI1, sul1, qnrS, and blaTEM, while blaCTXM-32 and vanA were below the limit of quantification in most or all the samples. For the genes 16S rRNA, sul1, intI1, blaTEM and qnrS the inter-laboratory variation was below 28% (3-8%, 6-18%, 8-21%, 10-24%, 15-28%, respectively). While it may be difficult to fully harmonize qPCR protocols due to equipment, reagents and operator variations, the inter-laboratory calibration is an adequate and necessary step to increase the reliability of comparative data on ARGs abundance in different environmental compartments and/or geographic regions.
Published: 2020
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46. Predicted concentrations of anticancer drugs in the aquatic environment: What should we monitor and where should we treat?

Author: Kashyap Kumar Dubey, Patricia Luis, V.J. Pereira, Mrinal Kanti Mandal, Ankush Yadav, M.B. Cristóvão, B. Van der Bruggen, Shailesh Pandey, João G. Crespo, Raphael Janssens, UCL - SST/IMMC/IMAP - Materials and process engineering, Instituto de Biologia Experimental e Tecnológica - iBET, Central University of Haryana - Bioprocess Engineering Laboratory, National Institute of Technology Durgapur - n/a, KULeuven - n/a, Universidade Nova de Lisboa - LAQV-REQUIMTE, and Universidade Nova de Lisboa - Instituto de Tecnologia Química e Biológica António Xavier
Subjects: Environmental Engineering, Hospitalized and outpatients, Health, Toxicology and Mutagenesis, 0211 other engineering and technologies, India, Antineoplastic Agents, Fresh Water, 02 engineering and technology, Wastewater, 010501 environmental sciences, Entry route, 01 natural sciences, Anticancer drugs, Feces, Consumption pattern, Humans, Environmental Chemistry, Effective treatment, Medicine, Waste Management and Disposal, 0105 earth and related environmental sciences, 021110 strategic, defence & security studies, Portugal, business.industry, Predicted environmental concentrations, Pollution, Monitoring program, Drug Utilization, Hospitals, Biotechnology, Aquatic environment, Housing, business, Water Pollutants, Chemical, Environmental Monitoring
Abstract: Anticancer drugs have been detected in the aquatic environment, they have a potent mechanism of action and their consumption is expected to drastically increase in the future. Consequently, it is crucial to routinely monitor the occurrence of anticancer drugs and to develop effective treatment options to avoid their release into the environment. Prior to implementing a monitoring program, it is important to define which anticancer drugs are more prone to be found in the surface waters. In this study the consumption of anticancer drugs in the Lisbon region (Portugal), Belgium and Haryana state (India) were used to estimate the concentrations that can be expected in surface waters. Moreover, one important aspect is to define the major entry route of anticancer drugs in the aquatic environment: is it hospital or household effluents? The results disclosed in this study showed that in Belgium and Lisbon, 94 % of the total amount of anticancer drugs were delivered to outpatients, indicating that household effluents are the primary input source of these drugs and thus, upgrading the treatment in the domestic wastewater facilities should be the focus.
Published: 2020

47. A lanthanide MOF immobilized in PMMA transparent films as a selective fluorescence sensor for nitroaromatic explosive vapours

Author: Moscoso F.G., Almeida J., Sousaraei A., Lopes-Costa T., Silva A.M.G., Cabanillas-Gonzalez J., Cunha-Silva L., Pedrosa J.M. and The authors are grateful to the Spanish Ministry of Science, Innovation and Universities for funding through projects PCIN-2015-169-C02-01/02 (MOFsENS, under the project M-Era-NET/0005/2014), and RTI2018-097508-B-I00 (AMAPOLA). JCG acknowledges support from the Regional Government of Madrid through NMAT2D-CM project (S2018/NMT-4511). IMDEA Nanociencia acknowledges support from the ‘‘Severo Ochoa’’ Programme for Centers of Excellence in RD (MINECO, Grant SEV-2016-0686). Funding from the Regional Goverment of Andalucia through the grant AT17-5549 also contributed to the present research. The Porto group thanks Fundação para a Ciência e a Tecnologia (FCT) for the financial support to the LAQV/REQUIMTE (UID/QUI/50006/2019) through national funds and co-financed by FEDER under the PT2020 partnership agreement, LCS and AMGS also acknowledge the FCT by the employment contracts (in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19) supported by national funds (OE). JA also acknowledges FCT for the PhD grant PD/BD/142868/2018.
Published: 2020

48. Luminescent MOF crystals embedded in PMMA/PDMS transparent films as effective NO2gas sensors

Author: Moscoso F.G., Almeida J., Sousaraei A., Lopes-Costa T., Silva A.M.G., Cabanillas-Gonzalez J., Cunha-Silva L., Pedrosa J.M. and The authors are grateful to the Spanish Ministry of Science, Innovation and Universities for funding through projects PCIN-2015-169-C02-01/02 (MOFsENS, under the project M-Era-NET/ 0005/2014) and RTI2018-097508-B-I00 (AMAPOLA). JCG acknowledges support from the Regional Government of Madrid through the NMAT2D-CM project (S2018/NMT-4511). IMDEA Nanociencia acknowledges support from the 'Severo Ochoa' Programme for Centers of Excellence in R&D (MINECO, Grant SEV-2016-0686). Funding from the Regional Government of Andalucia through the grant AT17-5549 also contributed to the present research. The Porto group thanks Fundação para a Ciência e a Tecnologia (FCT) for the financial support to the LAQV/REQUIMTE (UID/QUI/50006/2019) through national funds and co-financed by FEDER under the PT2020 partnership agreement. LCS and AMGS also acknowledge the FCT for the employment contracts (in the scope of the framework contract foreseen in numbers 4, 5 and 6 of article 23 of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19) supported by national funds (OE).
Published: 2020

49. Aligning the good practice mask with the objectives of the European innovation partnership on active and healthy ageing

Author: Bousquet, J. and Farrell, J. and Onorato, G.L. and Bedbrook, A. and Czarlewski, W. and Micheli, Y. and Arnavielhe, S. and Illario, M. and Ansotegui, I.J. and Anto, J.M. and Bachert, C. and Basagaña, X. and Bédard, A. and Benveniste, S. and Bergmann, K.C. and Bewick, M. and Bindslev-Jensen, C. and Bjermer, L. and Blain, H. and Bosnic-Anticevich, S. and Bosse, I. and Braido, F. and Brussino, L. and Camuzat, T. and Canonica, G.W. and Cardona, V. and Carreiro Martins, P. and Cecchi, L. and Chavannes, N.H. and Chu, D.K. and Correia da Sousa, J. and Costa, D.J. and Costa, E. and Cruz, A.A. and da Silva, J. and Devillier, P. and de Feo, G. and de Vries, G. and Dray, G. and Ebisawa, M. and Erhola, M. and Fauquert, J.L. and Fokkens, W.J. and Fonseca, J. and Fontaine, J.M. and Gemicioğlu, B. and Haahtela, T. and Heffler, E. and Hellings, P.W. and Ivancevich, J.C. and Jassem, E. and Jutel, M. and Kaidashev, I. and Kalayci, O. and Klimek, L. and Kowalski, M.L. and Kull, I. and Kuna, P. and Kvedariene, V. and la Grutta, S. and Laune, D. and Larenas-Linnemann, D. and Ierodiakonou, D. and Le, L.T.T. and Lourenço, O. and Makris, M. and Menditto, E. and Monti, R. and Morais-Almeida, M. and Münter, L. and Muraro, A. and Murray, R. and Maurer, M. and Melén, E. and Mösges, R. and Mullol, J. and Niedoszytko, M. and O'Hehir, R.E. and Okamoto, Y. and Papadopoulos, N.G. and Passalacqua, G. and Patella, V. and Pereira, A.M. and Pfaar, O. and Pham-Thi, N. and Portejoie, F. and Price, D. and Prokopakis, E.P. and Psarros, F. and Raciborski, F. and Regateiro, F. and Reitsma, S. and Roche, N. and Rolland, C. and Ryan, D. and Samolinski, B. and Sastre, J. and Scadding, G.K. and Schmid-Grendelmeier, P. and Schünemann, H.J. and Shamji, M. and Sheikh, A. and Stellato, C. and Suppli-Ulrik, C. and Somekh, D. and Sova, M. and Todo Bom, A. and Tomazic, P.V. and Toppila-Salmi, S. and Triggiani, M. and Tsiligianni, I. and Valero, A. and Valiulis, A. and Valovirta, E. and van Eerd, M. and Vasankari, T. and Ventura, M.T. and Wallace, D. and Waserman, S. and Yorgancioglu, A. and Zidarn, M. and Zuberbier, T., CHU Arnaud de Villeneuve, Montpellier, France, MACVIA-France, Montpellier, France, INSERM U 1168, VIMA: Ageing and Chronic Diseases Epidemiological and Public Health Approaches, Villejuif, France, UMR-S 1168, Université Versailles St-Quentin-en-Yvelines, Montigny le Bretonneux, France, Euforea, Brussels, Belgium, Charité - Universitätsmedizin Berlin, Humboldt-Universität zu Berlin, Berlin, Germany, Department of Dermatology and Allergy, Berlin Institute of Health, Comprehensive Allergy Center, Berlin, Germany, LANUA International Healthcare Consultancy, Belfast, Northern Ireland, United Kingdom, Medical Consulting Czarlewski, Levallois, France, KYomed INNOV, Montpellier, France, Division for Health Innovation, Campania Region and Federico II University Hospital Naples (R&D Unit and Department of Public Health), Naples, Italy, Department of Allergy and Immunology, Hospital Quirónsalud Bizkaia, Erandio, Spain, ISGlobAL, Centre for Research in Environmental Epidemiology (CREAL), Barcelona, Spain, Hospital del Mar Research Institute (IMIM), Barcelona, Spain, CIBER Epidemiología y Salud Pública (CIBERESP), Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Upper Airways Research Laboratory, Department of ENT, Ghent University Hospital, Ghent, Belgium, National Center of Expertise in Cognitive Stimulation (CEN STIMCO), Broca Hospital, Paris, France, Mines ParisTech CRI - PSL Research University, Fontainebleau, France, iQ4U Consultants Ltd, London, United Kingdom, Department of Dermatology and Allergy Centre, Odense University Hospital, Odense Research Center for Anaphylaxis (ORCA), Odense, Denmark, Department of Respiratory Medicine and Allergology, Lund University, Lund, Sweden, Department of Geriatrics, Montpellier University Hospital, Montpellier, France, EA 2991, Euromov, University, Montpellier, France, Woolcock Institute of Medical Research, University of Sydney and Woolcock Emphysema Centre and Sydney Local Health District, Glebe, Australia, La Rochelle, France, University of Genoa, Department of Internal Medicine, DiMI) and IRCCS Ospedale Policlinico San Martino, Genova, Italy, Department of Medical Sciences, Allergy and Clinical Immunology Unit, University of Torino, Mauriziano Hospital, Torino, Italy, Région Occitanie, Montpellier, France, Personalized Medicine Clinic Asthma and Allergy, Humanitas Clinical and Research Center IRCCS, Rozzano (MI), Italy, Allergy Section, Department of Internal Medicine, Hospital Vall d'Hebron and ARADyAL Research Network, Barcelona, Spain, Serviço de Imunoalergologia, Hospital de Dona Estefânia, Centro Hospitalar de Lisboa Central, Lisbon, Portugal, CEDOC-CHRC, Faculdade de Ciências Médicas (FCM), Universidade Nova de Lisboa, Lisbon, Portugal, SOS Allergology and Clinical Immunology, USL Toscana Centro, Prato, Italy, Department of Public Health and Primary Care, Leiden University Medical Center, Leiden, Netherlands, Department of Health Research Methods, Evidence, and Impact, McMaster University, Hamilton, Canada, Division of Clinical Immunology and Allergy, Department of Medicine, McMaster University, Hamilton, Canada, Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal, ICVS/3B's, PT Government Associate Laboratory, Braga/Guimarães, Portugal, Nîmes, France, UCIBIO, REQUIMTE, Faculty of Pharmacy, and Competence Center on Active and Healthy Ageing of University of Porto (AgeUPNetWork), University of Porto, Porto, Portugal, ProAR - Nucleo de Excelencia em Asma, Federal University of Bahia, Salvador, Brazil, WHO GARD Planning Group, Salvador, Brazil, Allergy Service, University Hospital of Federal University of Santa Catarina (HU-UFSC), Florianópolis, Brazil, UPRES EA220, Pôle des Maladies des Voies Respiratoires, Hôpital Foch, Université Paris-Saclay, Suresnes, France, Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Salerno, Italy, Peercode BV, Geldermalsen, Netherlands, IMT Mines Alès, Université Montpellier, France, Clinical Reserch Center for Allergy and Rheumatology, Sagamihara National Hospital, Sagamihara, Japan, National Insitute for Health and Welfare, Helsinki, Finland, CHU Clermont-Ferrand, Unité d'allergologie de l'enfant, Pôle pédiatrique, Hôpital Estaing, Clermont-Ferrand, France, Department of Otorhinolaryngology, Amsterdam University Medical Centres, AMC, Amsterdam, Netherlands, CINTESIS, Center for Research in Health Technology and Information Systems, Faculdade de Medicina da Universidade do Porto, Porto, Portugal, Medida, Lda, Porto, Portugal, Reims, France, Department of Pulmonary Diseases, Istanbul University-Cerrahpasa, Cerrahpasa Faculty of Medicine, Istambul, Turkey, Skin and Allergy Hospital, Helsinki University Hospital, University of Helsinki, Helsinki, Finland, Department of Otorhinolaryngology, University Hospitals Leuven, Leuven, Belgium, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands, Servicio de Alergia e Immunologia, Clinica Santa Isabel, Buenos Aires, Argentina, Department of Allergology, Medical University of Gdańsk, Gdańsk, Poland, Department of Clinical Immunology, Wrocław Medical University, Poland, Ukrainina Medical Stomatological Academy, Poltava, Ukraine, Pediatric Allergy and Asthma Unit, Hacettepe University School of Medicine, Ankara, Turkey, Center for Rhinology and Allergology, Wiesbaden, Germany, Department of Immunology and Allergy, Healthy Ageing Research Center, Medical University of Lodz, Poland, Department of Clinical Science and Education Södersjukhuset, Karolinska Institutet, Stockholm, Sweden, Sach's Children and Youth Hospital, Södersjukhuset, Stockholm, Sweden, Division of Internal Medicine, Asthma and Allergy, Barlicki University Hospital, Medical University of Lodz, Poland, Institute of Biomedical Sciences, Department of Pathology, Faculty of Medicine, Vilnius University, Institute of Clinical Medicine, Clinic of Chest diseases and Allergology, Faculty of Medicine, Vilnius, Lithuania, Institute for Research and Biomedical Innovation (IRIB), National Research Council (CNR), Palermo, Italy, Center of Excellence in Asthma and Allergy, Médica Sur Clinical Foundation and Hospital, México City, Mexico, Department of Social Medicine, Faculty of Medicine, University of Crete, International Primary Care Respiratory Group, Crete, Greece, International Primary Care Respiratory Group IPCRG, Aberdeen, United Kingdom, University of Medicine and Pharmacy, Ho Chi Minh City, Viet Nam, Faculty of Health Sciences and CICS - UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal, Allergy Unit 'D Kalogeromitros', 2nd Department of Dermatology and Venereology, National and Kapodistrian University of Athens, Attikon University Hospital, Athens, Greece, CIRFF, Center of Pharmacoeconomics, University of Naples Federico II, Naples, Italy, Allergy Center, CUF Descobertas Hospital, Lisbon, Portugal, Danish Commitee for Health Education, Copenhagen East, Denmark, Food Allergy Referral Centre Veneto Region, Department of Women and Child Health, Padua General University Hospital, Padua, Italy, MedScript, Paraparaumu, New Zealand, Optimum Patient Care, Cambridge, United Kingdom, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden, Institute of Medical Statistics, and Computational Biology, Medical Faculty, University of Cologne, Germany and CRI-Clinical Research International-Ltd, Hamburg, Germany, Rhinology Unit and Smell Clinic, Department of ENT, Hospital Clínic, Barcelona, Spain, Clinical and Experimental Respiratory Immunoallergy, IDIBAPS, CIBERES, University of Barcelona, Barcelona, Spain, Department of Allergy, Immunology and Respiratory Medicine, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Australia, Department of Immunology, Monash University, Melbourne, Australia, Department of Otorhinolaryngology, Chiba University Hospital, Chiba, Japan, Division of Infection, Immunity and Respiratory Medicine, Royal Manchester Children's Hospital, University of Manchester, Manchester, United Kingdom, Department of Allergy, 2nd Pediatric Clinic, Athens General Children's Hospital 'P&A Kyriakou,', University of Athens, Athens, Greece, Allergy and Respiratory Diseases, Ospedale Policlino San Martino, University of Genoa, Italy, Division of Allergy and Clinical Immunology, Department of Medicine, Agency of Health ASL Salerno, Santa Maria della Speranza Hospital, Salerno, Italy, Allergy Unit, CUF-Porto Hospital and Institute, Porto, Portugal, Department of Otorhinolaryngology, Head and Neck Surgery, Section of Rhinology and Allergy, University Hospital Marburg, Philipps-Universität Marburg, Germany, Department of Allergy, Pasteur Institute, Paris, France, Observational and Pragmatic Research Institute, Singapore, Singapore, Department of Otorhinolaryngology, University of Crete, School of Medicine, Heraklion, Greece, Allergy Department, Athens Naval Hospital, Athens, Greece, Department of Prevention of Envinronmental Hazards and Allergology, Medical University of Warsaw, Warsaw, Poland, Imunoalergologia, Centro Hospitalar Universitário de Coimbra, Faculty of Medicine, University of Coimbra, Portugal, Pneumologie et Soins Intensifs Respiratoires, Hôpitaux Universitaires Paris, Centre Hôpital Cochin, Paris, France, Association Asthme et Allergie, Paris, France, Allergy and Respiratory Research Group, University of Edinburgh, Edinburgh, United Kingdom, Faculty of Medicine, Autnonous University of Madrid, Spain, Royal National TNE Hospital, University College London, London, United Kingdom, Allergy Unit, Department of Dermatology, University Hospital of Zurich, Zürich, Switzerland, Immunomodulation and Tolerance Group, Allergy and Clinical Immunology, Imperial College London, London, United Kingdom, Usher Institute of Population Health Sciences and Informatics, University of Edinburgh, Edinburgh, United Kingdom, Department of Respiratory Medicine, Hvidovre Hospital, University of Copenhagen, Copenhagen, Denmark, European Health Futures Forum (EHFF), Dromahair, Ireland, Department of Respiratory Medicine, University Hospital Olomouc, Olomouc, Czech Republic, Imunoalergologia, Centro Hospitalar Universitário de Coimbra, Faculty of Medicine, University of Coimbra, Coimbra, Portugal, Department of General ORL, H1NS, Medical University of Graz, Graz, Austria, Vilnius University Faculty of Medicine, Institute of Clinical Medicine, Institute of Health Sciences, Vilnius, Lithuania, European Academy of Paediatrics (EAP/UEMS-SP), Brussels, Belgium, Department of Lung Diseases and Clinical Immunology, University of Turku and Terveystalo Allergy Clinic, Turku, Finland, FILHA, Finnish Lung Association, Helsinki, Finland, University of Bari Medical School, Unit of Geriatric Immunoallergology, Bari, Italy, Nova Southeastern University, Fort Lauderdale, FL, United States, Department of Pulmonary Diseases, Celal Bayar University, Faculty of Medicine, Manisa, Turkey, and University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia
Abstract: The reference sites of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA) were renewed in 2019. The DG Santé good practice Mobile Airways Sentinel networK was reviewed to meet the objectives of the EIP on AHA. It included 1) Management of care process, 2) Blueprint of digital transformation, 3) EIP on AHA, innovation to market, 4) Community for monitoring and assessment framework, 5) Political, organizational, technological and financial readiness, 6) Contributing to European co-operation and transferability, 7) Delivering evidence of impact against the triple win approach, 8) Contribution to the European Digital Transformation of Health and Care and 9) scale of demonstration and deployment of innovation. © 2020 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https:// creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Published: 2020

50. Electrochemical CO2 reduction reaction on cost-effective oxide-derived copper and transition metal-nitrogen-carbon catalysts

Author: Jonathan Albo, Lin Zhang, Carlos M. Sánchez-Sánchez, Ivan Merino-Garcia, Universidad de Cantabria, Laboratoire Interfaces et Systèmes Electrochimiques (LISE), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto, Univ Cantabria, Chem & Blomol Engn Dept, Av Los Castros, E-39005 Santander, Spain, and Labex MATISSE (ASDB/2017-12)
Subjects: Transition metal–nitrogen–carbon catalysts, Materials science, Carbon dioxide electrochemical reduction reaction, Oxide, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, Electrochemistry, Electrocatalyst, 7. Clean energy, 01 natural sciences, Redox, Analytical Chemistry, Catalysis, chemistry.chemical_compound, Transition metal, Transition metal-nitrogen-carbon catalysts, [CHIM]Chemical Sciences, Oxide-derived copper catalysts, Value-added products, [CHIM.CATA]Chemical Sciences/Catalysis, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, Chemical engineering, 0210 nano-technology, Electrocatalysis, Carbon, Carbon monoxide
Abstract: The electrochemical CO2 reduction reaction (CO2RR) either to generate multicarbon (C2+) or single carbon (C1) value-added products provides an effective and promising approach to mitigate the high CO2 concentration in the atmosphere and promote energy storage. However, cost-effectiveness of catalytic materials limits practical application of this technology in the short term. Herein, we summarize and discuss recent and advanced works on cost-effective oxide-derived copper catalysts for the generation of C2+ products (hydrocarbons and alcohols) and transition metal-nitrogen-doped carbon electrocatalytic materials for C1 compounds production from CO2RR. We think they represent suitable electrocatalyst candidates for scaling up electrochemical CO2 conversion. This short review may provide inspiration for the future design and development of innovative active, cost-effective, selective and stable electrocatalysts with improved properties for either the production of C2+ (alcohols, hydrocarbons) or carbon monoxide from CO2RR. C.M. S.–S. thanks the funding of Sorbonne Université - China Scholarship Council program 2017 and Labex Matisse program (ASDB/2017-12) for the PhD contract of L. Zhang, as well as the ECOS Sud-Chile CONICYT program (Project C17E10). Thanks for the help of Dr. F. J. Recio Cortes and Dr. R. Venegas in Pontificia Universidad Católica de Chile. I.M.-G. would like to thank his postdoctoral contract in the frame of Research Project PTDC/EQU-EPQ/29579/2017, financed by ‘Programa Operacional Regional de Lisboa, na componente FEDER’ and ‘Fundação para a Ciência e Tecnologia, I.P’. J.A. gratefully acknowledge the Spanish Ministry of Economy and Competitiveness (MINECO) under Ramón y Cajal program (RYC-2015-17080).
Published: 2020
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