Search

Your search keyword '"Picard, Emmanuel"' showing total 31 results
Start Over Author "Picard, Emmanuel" Publication Year Range Last 10 years
31 results on '"Picard, Emmanuel"'

8. Tunable optical lattices in the near-field of a few-mode nanophotonic waveguide

Author

Pin Christophe, Jager Jean-Baptiste, Tardif Manon, Picard Emmanuel, Hadji Emmanuel, de Fornel Frédérique, and Cluzel Benoît

Subjects
Physics, QC1-999
Abstract

Due to the action of the scattering force, particles that are optically trapped at the surface of a waveguide are propelled in the direction of the light propagation. In this work, we demonstrate an original approach for creating tunable periodic arrays of optical traps along a few-mode silicon nanophotonic waveguide. We show how the near-field optical forces at the surface of the waveguide are periodically modulated when two guided modes with different propagation constants are simultaneously excited. The phenomenon is used to achieve stable trapping of a large number of dielectric particles or bacteria along a single waveguide. By controlling the light coupling conditions and the laser wavelength, we investigate several techniques for manipulating the trapped particles. Especially, we demonstrate that the period of the optical lattice can be finely tuned by adjusting the laser wavelength. This effect can be used to control the trap positions, and thus transport the trapped particles in both directions along the waveguide.

Published
2019
Full Text
View/download PDF

11. Phage susceptibility testing and infectious titer determination through wide-field lensless monitoring of phage plaque growth

Author

Perlemoine, Prisca, Marcoux, Pierre, Picard, Emmanuel, Hadji, Emmanuel, Zelsmann, Marc, Mugnier, Grégoire, Marchet, Aurélie, Resch, Grégory, O’Connell, Larry, Lacot, Eric, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), PHotonique, ELectronique et Ingénierie QuantiqueS (PHELIQS), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Laboratoire des technologies de la microélectronique (LTM ), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Department of Fundamental Microbiology [Lausanne], Université de Lausanne (UNIL), Chimie pour la Reconnaissance et l’Etude d’Assemblages Biologiques (CREAB ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Département Interfaces pour l'énergie, la Santé et l'Environnement (DIESE), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), and Université de Lausanne = University of Lausanne (UNIL)

Subjects
Time Factors, Lysis (Medicine), [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Imaging Techniques, Physiology, Science, Staphylococcus, viruses, Geometry, Pathology and Laboratory Medicine, Research and Analysis Methods, Microbiology, Klebsiella Pneumoniae, Klebsiella, Microbial Control, Tissue Repair, Image Processing, Computer-Assisted, Medicine and Health Sciences, Prototypes, Bacteriophages, Staphylococcus Aureus, Microbial Pathogens, Lenses, Pharmacology, Bacteria, Organisms, Biology and Life Sciences, Bacterial Pathogens, Kinetics, Technology Development, Radii, Medical Microbiology, Antibiotic Resistance, Viruses, Physical Sciences, Medicine, Engineering and Technology, Antimicrobial Resistance, Pathogens, Physiological Processes, Mathematics, Research Article
Abstract

International audience; The growing number of drug-resistant bacterial infections worldwide is driving renewed interest in phage therapy. Based on the use of a personalized cocktail composed of highly specific bacterial viruses, this therapy relies on a range of tests on agar media to determine the most active phage on a given bacterial target (phage susceptibility testing), or to isolate new lytic phages from an environmental sample (enrichment of phage banks). However, these culture-based techniques are still solely interpreted through direct visual detection of plaques. The main objective of this work is to investigate computer-assisted methods inorder to ease and accelerate diagnosis in phage therapy but also to study phage plaque growth kinetics. For this purpose, we designed a custom wide-field lensless imaging device, which allows continuous monitoring over a very large area sensor (3.3 cm$^2$ ). Here we report bacterial susceptibility to $Staphylococcus\ aureus$ phage in 3 hr and estimation of infectious titer in 8 hr 20 min. These are much shorter time-to-results than the 12 to 24 hours traditionally needed, since naked eye observation and counting of phage plaques is still the most widely used technique for susceptibility testing prior to phage therapy. Moreover, the continuous monitoring of the samples enables the study of plaque growth kinetics, which enables a deeper understanding of the interaction between phage and bacteria. Finally, thanks to the 4.3 μm resolution, we detect phage-resistant bacterial microcolonies of $Klebsiella\ pneumoniae$ inside the boundaries of phage plaques and thus show that our prototype is also asuitable device to track phage resistance. Lensless imaging is therefore an all-in-one method that could easily be implemented in cost-effective and compact devices in phage laboratories to help with phage therapy diagnosis.

Published
2021

12. Artificial intelligence and large field, high-resolution lensless imaging for non-destructive, label-free identification directly on agar

Author

Schiavone, Patrick, Zamoun, Inès, Soulan, Sébastien, Maire, Alexis, Gougis, Maxime, Marcoux, Pierre R., Yescas González, Tonatiuh, Mugnier, Grégoire, Picard, Emmanuel, Zelsmann, M., Hadji, Emmanuel, Peyrade, David, Schiavone, Patrick, Laboratoire des technologies de la microélectronique (LTM ), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

13. 'Stable particle clusters trapped and assembled using a plasmonic nanotweezer integrated on a silicon waveguide,' oral (on-line) presentation

Author

Pin, Christophe, Magno, Giovanni, Ecarnot, Aurore, Picard, Emmanuel, Hadji, Emmanuel, Vy, Yam, De Fornel, Frederique, Dagens, Beatrice, Cluzel, Benoit, Centre de Nanosciences et de Nanotechnologies (C2N), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and DAGENS, Béatrice

Subjects
[PHYS]Physics [physics], ComputingMilieux_MISCELLANEOUS, [PHYS] Physics [physics]
Abstract

International audience

Published
2020

21. On-chip photonic tweezers for photonics, microfluidics, and biology

Author

Omatsu, Takashige, Pin, Christophe, Renaut, Claude, Tardif, Manon, Jager, Jean-Baptiste, Delamadeleine, Eric, Picard, Emmanuel, Peyrade, David, Hadji, Emmanuel, De Fornel, Frédérique, Cluzel, Benoit, Chiba University, Laboratoire des technologies de la microélectronique (LTM ), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire Interdisciplinaire Carnot de Bourgogne [Dijon] (LICB), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Université de Technologie de Belfort-Montbeliard (UTBM), Silicon Nanoelectronics Photonics and Structures (SiNaps), PHotonique, ELectronique et Ingénierie QuantiqueS (PHELIQS), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Interdisciplinaire Carnot de Bourgogne (LICB), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des technologies de la microélectronique [2001-2015] (LTM [2001-2015]), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Service de Physique des Matériaux et Microstructures (SP2M - UMR 9002), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), and Agence Universitaire de la Francophonie (AUF)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, Materials science, Microfluidics, Physics::Optics, Nanotechnology, Near and far field, 02 engineering and technology, 01 natural sciences, Optofluidics, law.invention, law, 0103 physical sciences, Tweezers, Physics::Atomic Physics, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, ComputingMilieux_MISCELLANEOUS, 010302 applied physics, Condensed Matter::Quantum Gases, [PHYS]Physics [physics], Silicon photonics, business.industry, Lab-on-a-chip, 021001 nanoscience & nanotechnology, [SPI.ELEC]Engineering Sciences [physics]/Electromagnetism, Optical tweezers, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Photonics, 0210 nano-technology, business
Abstract

International audience; Near-field optical forces arise from evanescent electromagnetic fields and can be advantageously used for on-chip optical trapping. In this work, we investigate how evanescent fields at the surface of photonic cavities can efficiently trap micro-objects such as polystyrene particles and bacteria. We study first the influence of trapped particle’s size on the trapping potential and introduce an original optofluidic near-field optical microscopy technique. Then we analyze the rotational motion of trapped clusters of microparticles and investigate their possible use as microfluidic micro-tools such as integrated micro-flow vane. Eventually, we demonstrate efficient on-chip optical trapping of various kinds of bacteria.

Published
2017

22. Optical trapping and Gram type differentiation of living bacteria with 2D photonic crystal cavities

Author

Therisod, Rita, Tardif, Manon, Picard, Emmanuel, Marcoux, Pierre R., Gaude, Victor, Jager, Jean-Baptiste, Hadji, Emmanuel, Peyrade, David, Houdré, Romuald, Laboratoire des technologies de la microélectronique (LTM ), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Silicon Nanoelectronics Photonics and Structures (SiNaps), PHotonique, ELectronique et Ingénierie QuantiqueS (PHELIQS), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Laboratoire de Génie Electrique de Grenoble (G2ELab), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut de Photonique et d'Electronique Quantiques, and Ecole Polytechnique Fédérale de Lausanne (EPFL)

Subjects
[PHYS]Physics [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience; The development of methods for the rapid analysis of pathogenic bacteria or viruses is of crucial interest in the clinical diagnosis of infectious diseases. In the last decade, optical resonators integrated with microfluidic layers arose as promising tools for biological analysis, notably thanks to their ability to trap objects with low powers, beneath the damage threshold of biological entities, and with a small footprint. Moreover, the resonant nature of optical cavities allows for the simultaneous acquisition of information on the trapped objects, thanks to the feedback effect induced by the specimen on the trapping field itself. Here we report on the trapping and on the Gram-type differentiation of seven types of living bacteria in an optofluidic system based on an optical cavity consisting in a large hole in a 2D silicon photonic crystal membrane. The hollow nature of the resonant cavity results in a large overlap between the confined field and the hollow volume, allowing for a maximum interaction between the trapping field and the trapped cell. The optical cavity was excited at the resonance wavelength and the shift induced by the trapped bacteria was analysed. To test the trapping capabilities of our structure, we investigated seven types of bacteria, featuring different morphologies, Gram-types and mobilities (presence or absence of flagella). The analysis of the resonance shift yielded Gram typing in a label-free and not destructive way, due to differences in the refractive index and in the deformability of the cell wall. In particular, Gram negative bacteria showed a larger shift.

Published
2017

28. On-chip photonic tweezers for photonics, microfluidics, and biology

Author

Pin, Christophe, additional, Renaut, Claude, additional, Tardif, Manon, additional, Jager, Jean-Baptiste, additional, Delamadeleine, Eric, additional, Picard, Emmanuel, additional, Peyrade, David, additional, Hadji, Emmanuel, additional, de Fornel, Frédérique, additional, and Cluzel, Benoît, additional

Published
2017
Full Text
View/download PDF

31. Phage susceptibility testing and infectious titer determination through wide-field lensless monitoring of phage plaque growth.

Author

Perlemoine P, Marcoux PR, Picard E, Hadji E, Zelsmann M, Mugnier G, Marchet A, Resch G, O'Connell L, and Lacot E

Subjects
Bacteria virology, Kinetics, Time Factors, Bacteriophages growth & development, Image Processing, Computer-Assisted, Lenses
Abstract

The growing number of drug-resistant bacterial infections worldwide is driving renewed interest in phage therapy. Based on the use of a personalized cocktail composed of highly specific bacterial viruses, this therapy relies on a range of tests on agar media to determine the most active phage on a given bacterial target (phage susceptibility testing), or to isolate new lytic phages from an environmental sample (enrichment of phage banks). However, these culture-based techniques are still solely interpreted through direct visual detection of plaques. The main objective of this work is to investigate computer-assisted methods in order to ease and accelerate diagnosis in phage therapy but also to study phage plaque growth kinetics. For this purpose, we designed a custom wide-field lensless imaging device, which allows continuous monitoring over a very large area sensor (3.3 cm2). Here we report bacterial susceptibility to Staphylococcus aureus phage in 3 hr and estimation of infectious titer in 8 hr 20 min. These are much shorter time-to-results than the 12 to 24 hours traditionally needed, since naked eye observation and counting of phage plaques is still the most widely used technique for susceptibility testing prior to phage therapy. Moreover, the continuous monitoring of the samples enables the study of plaque growth kinetics, which enables a deeper understanding of the interaction between phage and bacteria. Finally, thanks to the 4.3 μm resolution, we detect phage-resistant bacterial microcolonies of Klebsiella pneumoniae inside the boundaries of phage plaques and thus show that our prototype is also a suitable device to track phage resistance. Lensless imaging is therefore an all-in-one method that could easily be implemented in cost-effective and compact devices in phage laboratories to help with phage therapy diagnosis., Competing Interests: The authors have declared that no competing interests exist.

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results