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Abstract This study is focused on analysing polyphenols and carbohydrates released by Phaeodactylum tricornutum (P. tricornutum) diatoms cultured in natural seawater enriched with sublethal and lethal Cu doses. Cu concentrations of 0.31, 0.79 and 1.57 µM reduced cell densities by 37, 82 and 91%, respectively, compared to the control. The total sum of all identified polyphenols and total carbohydrates released by cells grown under lethal Cu levels increased up to 18.8 and 107.4 times, respectively, compared to data from a control experiment. Four different in vitro assays were used to estimate the antioxidant activities of the extracellular compounds: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition, cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power and Cu complexing ability (CCA). The highest antioxidant activities were observed in the Cu lethal treatments, where the CCA assay exhibited a greater increase (up to 32.2 times higher than that found in the control experiment) to reduce the concentration of free Cu in the medium and its toxicity. The presence of Cu stimulated the release of polyphenols and carbohydrates to the medium as a detoxification mechanism to survive under lethal levels of Cu regulating its speciation.

Com o advento da Convenção Internacional sobre os Direitos das Pessoas com Deficiência (CDPD) no ordenamento jurídico brasileiro, o legislador criou a Lei Brasileira de Inclusão, a qual trouxe uma revolução na teoria das incapacidades e no instituto assistencial da curatela, levando a diversas críticas e questionamentos, principalmente após a entrada em vigência do Código de Processo Civil, que demonstrou a existência de antinomia jurídica das disposições normativas quanto ao instituto da curatela. Deste modo, como resposta a essas críticas e para dar fim à insegurança jurídica advinda da antinomia, apresentou-se o Projeto de Lei nº. 757/2015, que busca alterar o Código Civil e o Código de Processo Civil. Todavia, quando do seu texto inicial, questionamentos sobre a sua constitucionalidade frente à CDPD foram realizados, de modo que é essencial a análise desta conforme o texto atual do projeto de lei.

The biochemical composition of three novel selected microalgae strains (Chlorophyta) was evaluated to confirm their potential possibilities as new sustainably produced biomass with nutritional, functional, and/or biomedical properties. Extracts from cultured Pseudopediastrum boryanum, Chloromonas cf. reticulata, and Chloroidium saccharophilum exhibited higher radical scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazyl) when compared to butylated hydroxytoluene (BHT), but lower than butylated hydroxyanisole (BHA). Total phenolic compounds and amino acids were determined by newly developed RP-HPLC methods. Total phenolic contents, as µg g−1 of dry biomass, reached 27.1 for C. cf. reticulata, 26.4 for P. boryanum, and 55.8 for C. saccharophilum. Percentages of total analysed amino acids were 24.3, 32.1, and 18.5% of dry biomass, respectively, presenting high values for essential amino acids reaching 54.1, 72.6, and 61.2%, respectively. Glutamic acid was the most abundant free amino acid in all microalgae samples, followed by proline and lysine in C. saccharophilum and P. boryanum, and methionine and lysine in C. reticulata. Soluble carbohydrates in aqueous extracts ranged from 39.6 for C. saccharophilum to 49.3% for C. reticulata, increasing values to 45.1 for C. saccharophilum and 52.7% for P. boryanum in acid hydrolysates of dried biomass. Results confirmed the potential possibilities of these microalgae strains.

Este artículo propone la teoría del cuidado como medio para la creación de infraestructuras de sustento en los modelos comunes. Se aborda la manera en la que los espacios comunes en la urbe proporcionan contexto y soporte para una creación artística alternativa. Para ello se realiza un análisis de los diferentes modelos, privado, público y común, en relación a la creación del espacio urbano y se identifican sus posibilidades de relación entre ellos. La teoría del cuidado se plantea como la metodología que prima en la gestión de los comunes al proponer una reivindicación de las labores reproductivas y de sostenimiento. A partir del estudio de varios proyectos, este artículo establece una clasificación de dos tipologías de intersección entre práctica artística contemporánea y espacios comunes, separando los proyectos artísticos que se constituyen como comunes, de los espacios comunes que ofrecen una infraestructura para la creación. También se analiza la relación que los proyectos analizados mantienen con el sistema público, desde la no interacción entre ambos, hasta la subvención de recursos. El objetivo de este análisis es el de mostrar la capacidad que los comunes proporcionan como infraestructura relacional y material para la creación artística.