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Understanding beef cattle enteric emissions on extensive western U.S. rangeland systems is critical for implementing sustainable grazing practices that reduce greenhouse gas (GHG) emissions. These practices must also improve livestock and plant productivity and climate resiliency while maintaining or reducing production costs. However, enteric emissions of grazing cattle under different stocking rates and grazing management strategies are largely unknown for western U.S. rangelands. Thus, our objective was to evaluate the differences in enteric emissions of stocker steers grazing native rangeland. Yearling Angus steers (n = 127) were stratified by initial body weight (BW) to six groups, then randomly assigned to one of six pastures grazed from June to September 2023 at the South Dakota State University Cottonwood Field Station (Cottonwood, SD) as part of a long-term stocking rate trial (80+ yr). Each pasture was equipped with a GreenFeed (C-Lock Inc, Rapid City, SD) pasture system to measure individual animal CH4 and CO2 emissions for continuous grazing (CG) and rotational grazing (RG) strategies in three stocking rates (Light, Moderate, and Heavy, 0.32, 0.40, and 0.72 animal unit months, respectively) in a 2 x 3 factorial design. Differences in enteric emissions among stocking rate and grazing strategy were analyzed using a linear mixed effects model in R, using the lme4 package, with animal as a random effect, and a post-hoc contrast among treatments was assessed using the lsmeans package. Steer initial and final BW were similar (P > 0.05) among treatments. For CH4 emissions, there was a significant interaction between stocking rate and grazing strategy (P < 0.05). Specifically, CH4 emissions for moderate-RG (195 ± 4.3 g/d) were less than those from heavy-CG (221 ± 4.5 g/d) and light-RG (221 ± 4.7 g/d) over the study period (P < 0.05). For CO2 emissions, light-CG steers respired greater CO2 emissions (6892 ± 101 g/d) than heavy-RG (6398 ± 107 g/d; P < 0.05). All other treatment lsmeans were similar (P > 0.05) and intermediate to these treatment effects. The assessment of GHG emission values for cattle grazing native rangeland is essential for informing climate mitigation strategies, improving animal efficiency, and understanding changes in GHG emissions over production phases and across animal classes. [ABSTRACT FROM AUTHOR]

Our objective was to evaluate how virtual fencing grazing management during the stocker phase influences steer growth performance, carcass traits, and enteric emissions during the finishing phase. Angus steers [n = 96; initial body weight (BW) = 375 ± 7.2 kg) grazed native rangeland from 07 June 2023 to 31 August 2023 in Cottonwood, SD at South Dakota State University’s Cottonwood Field Station. Following summer grazing, steers were transported to Brookings, SD and fed for 130-d at the South Dakota State University Cow-Calf Education and Research Facility. During the summer grazing season, steers were assigned to different stocking rates (Light, Moderate, and Heavy, at 0.32, 0.42, and 0.72 animal unit months, respectively), and grazing strategies [continuous (CG) or virtual fencing rotational (RG)] in a 2 × 3 factorial design. Steers were transitioned from a 30% roughage to a 10% roughage diet over 21-d. The finishing diet (13.7% CP, 17.2% NDF, 1.36 Mcal/kg NEg, and 30 g/907-kg monensin sodium) was manufactured twice daily and provided ad libitum. Feed consumption was tracked using precision monitoring equipment (Insentec, The Hague, Netherlands). Steers were individually weighed on d 1 (entry BW), 21, 63, 90, and 130 and administered a steroidal implant (200 mg trenbolone acetate and 20 mg estradiol; Revalor-200, Merck Animal Health, DeSoto, KS) on d 21. Enteric emissions, including methane and carbon dioxide, were monitored using the GreenFeed trailer system (C-Lock Inc., Rapid City, SD), growth performance, carcass traits, and categorical carcass traits were analyzed in a factorial design using a randomized complete block design with individual steer considered the experimental unit. Final BW was determined from hot carcass weight divided by 0.625. Steers from heavy-CG had greater feedlot entry BW (P ≤ 0.05) compared with all treatments except light-CG and light-RG. Light-CG and light-RG had greater entry BW compared with heavy-RG (P ≤ 0.05). Gain efficiency was greater for light-CG compared with light-RG, moderate-CG, and heavy-CG (P ≤ 0.05), while moderate-RG and heavy-RG were intermediate (P ≤ 0.05). Steers from moderate-RG had greater marbling scores compared with heavy-RG (P = 0.05), and all other treatments were intermediate. Neither stocking density (P ≥ 0.15) nor grazing management strategy (P ≥ 0.13) had any influence on other measures of growth performance, emissions (CH4 and CO2 ) production rate, or carcass traits. These findings suggest that stocking density and grazing-management strategy (CG vs. virtual fencing-RG) influence gains on pasture and alter feedlot entry BW, highlighting the need for further investigation into their implications for greenhouse gas emissions, as well as for determining optimal stocking densities and management strategies for ranchers [ABSTRACT FROM AUTHOR]

Advancements in technology have ushered in a new era of sensor-based measurement and management of livestock production systems. These sensor-based technologies have the ability to automatically monitor feeding, growth, and enteric emissions for individual animals across confined and extensive production systems. One challenge with sensor-based technologies is the large amount of data generated, which can be difficult to access, process, visualize, and monitor information in real time to ensure equipment is working properly and animals are utilizing it correctly. A solution to this problem is the development of application programming interfaces (APIs) to automate downloading, visualizing, and summarizing datasets generated from precision livestock technology. For this methods paper, we develop three APIs and accompanying processes for rapid data acquisition, visualization, systems tracking, and summary statistics for three technologies (SmartScale, SmartFeed, and GreenFeed) manufactured by C-Lock Inc (Rapid City, SD). Program R markdown documents and example datasets are provided to facilitate greater adoption of these techniques and to further advance precision livestock technology. The methodology presented successfully downloaded data from the cloud and generated a series of visualizations to conduct systems checks, animal usage rates, and calculate summary statistics. These tools will be essential for further adoption of precision technology. There is huge potential to further leverage APIs to incorporate a wide range of datasets such as weather data, animal locations, and sensor data to facilitate decision-making on times scales relevant to researchers and livestock managers. [ABSTRACT FROM AUTHOR]

Spatial utilization of pasture landscapes by grazing animals is often heterogeneous and driven by complex environmental and physiological interactions between plants and animals. Synergistic energy pathways can be modeled using mechanistic relationships describing resource distribution, animal behavior, metabolic energy rate, and pasture utilization using biometric sensors. The objective of this study was to demonstrate the ability to measure the energy landscape by mapping fine-scale contributions to animal energy expenditure, nutrient acquisition, growth, and overall economic profitability. We utilized animal trajectories and location fixes recorded by virtual fencing collars (Vence) daily body weight (BW) using precision livestock scales (Smartscale, C-Lock Inc.) and oxygen consumption (GreenFeeds, C-Lock Inc.) on steers (n = 127), managed as part of a broader project to evaluate environmental synergies in extensive grazing systems. Steers were allocated to one of six native grass pastures assigned to either rotational or continuous grazing strategies, and one of three stocking rates (low, moderate, high) in a 2 x 3 factorial arrangement. Space utilization was quantified as the frequency of fixes located within each cell of a 5 x 5-meter Grid and the distance between grid cells and water was calculated using the Pythagorean theorem. Movement behavior was quantified using Program R to calculate step length, the distance between two temporally continuous fix points utilizing the Pythagorean Theorem, and turn angles calculated as the cosine of the pre- and pro-ceeding step lengths where larger values indicated greater deviation from a straight trajectory. A mixed linear regression model was fitted using the lmer function in the lme4 package. Space utilization decreased with increased distance from water (P < 0.05), with rotational grazing strategies increasing concentration around water sources (P < 0.05). This indicates an opportunity to utilize precision livestock technology to facilitate pasture management and increase space utilization in grazing cattle. [ABSTRACT FROM AUTHOR]

Solving complex livestock production problems is a pressing issue for achieving long-term sustainability and profitability and often requires modeling techniques. The use of mathematical models is undoubtedly a daily practice in the livestock industry, especially nutrition, and has improved animal performance, productivity, and environmental sustainability while maintaining or reducing costs. Although many students and professionals use spreadsheets and existing empirical models for nutrition and management [NASEM (2016)], there is still a need to understand the complexity of livestock systems and the utility of flight simulator models. At the same time, more complex models (although robust) may fail to provide new insights for experienced nutritionists due to poor userfriendliness. A systems understanding goes beyond simply obtaining a desired output, such as optimizing a total mixed ration, but instead leads to identifying high-leverage solutions and gaining insight. Further, parameterizing and calibrating variables and equations and testing management scenarios is straightforward. However, developing causal feedback linkages (A to B and B to A) and identifying time delays is less intuitive and more challenging for novices. Model flight simulators grounded in fully documented, calibrated models provide a means to introduce practitioners to a methodology of insight generation because the user designs and runs the model scenarios for themselves, challenging their mental models. Such approaches are generally more impactful (compared with someone telling them) because they have gained insight into the system themselves, and, in the case of open source (white box models), they can explore equations and parameters. Therefore, understanding how to utilize dynamic models in scenario-based simulations is critical in training current and future modelers in animal science. This hands-on model training will cover the basics of System Dynamics modeling and allow participants to run real-time animal production simulations. Finally, participants will be “debriefed” to unpack “ah ha” moments that were unexpected. The debrief will include using models to develop accurate guidelines and recommendations for those who cannot use computer models and developing experimental designs to test hypotheses and “validate” model recommendations (proof in the pudding). Participants will gain knowledge of System Dynamics applications for animal production systems, experience using flight simulators, and their utility in teaching, informing, and guiding their livestock production or that of a client. The “flight simulator” will focus on production and nutrition with species-agnostic principles. Participants will also have a new tool to identify areas for improvement in model development (i.e., what is missing?), research questions, or industry needs. The need for modelers who can turn big data into insight, knowledge, and wisdom using a systems approach is becoming even more critical due to the increasing use of precision livestock farming. Thus, providing the livestock industry with trained systems modelers will help achieve current and future sustainability challenges. [ABSTRACT FROM AUTHOR]

Virtual fencing (VF) has the capacity to transform the landscape of livestock management within extensive rangeland systems. The objective of this study was to evaluate the effect of Vence (Merck & Co., Inc., Rahway, NJ) VF technology on yearling steer behavior and performance. Steers were allocated into one of two grazing systems [continuous grazing (CG) or rotational grazing (RG) managed using VF] across three different stocking rates (light, moderate, and heavy, 0.32, 0.40, and 0.72 animal unit months, respectively) for three grazing seasons. Based on initial body weight, steers were randomly blocked into six pastures (346 ± 39, 273 ± 34, and 319 ± 29 kg, respectively) at the South Dakota State University Cottonwood Field Station (n = 127, 135, and 127, in 2021, 2022 and 2023, respectively). Steers assigned to the CG treatment had free access to the entire pasture for the duration of the grazing season. Steers assigned to the RG treatment were rotated among virtual ‘paddocks’ for the duration of the grazing season. The days spent grazing in each paddock for the RG treatment were determined based on bi-weekly clip plots for biomass estimation. The VF collars were used to track steer location at 5-min intervals; only steers assigned to the RG treatment were managed with VF boundaries and exposed to auditory and electrical cues from the collars. Raw data was downloaded from Vence Herd Manager through an automatic programming interface and cleaned by removing messages that failed to transmit correctly or were outside the bounds of study site pastures. Animal behavior metrics of daily distance traveled (DDT), daily grazing time, daily resting time, and daily walking time were calculated for each individual animal and averaged by pasture using Program R. Steer weights were collected using SmartScales (C-Lock Inc, Rapid City, SD). Average daily gain (ADG) was calculated using a linear model to develop a regression equation for each individual animal using weight as the dependent variable and day of trial as the independent variable. The slope of the regression line was used to estimate ADG. Individual DDT, behavior, and performance metrics were analyzed using a mixed model analysis of variance (ANOVA) to compare the impact of grazing treatment and stocking rate (fixed effects) with year as the random effect. Treatment did not influence (P > 0.05) grazing, walking, or resting behavior. Similarly, no differences were detected in DDT between treatment groups or across different stocking rates. Furthermore, there were no differences (P > 0.05) in ADG across treatment groups. Implementation of VF had no impact on animal behavior or performance, justifying its use in grazing settings; however, financial and cattle finishing aspects still need to be evaluated to determine its overall benefits and costs. [ABSTRACT FROM AUTHOR]

Predicting dry matter intake (DMI) for beef cattle on extensive rangelands presents a significant challenge to determining stocking rates. Traditionally, DMI is estimated by taking full body weight (BW) multiplied by a percentage selected based on animal class, production phase, and forage quality, which introduces tremendous levels of accumulated error at the herd level. Animal Unit Months (AUMs) are utilized to simplify the determination of stocking rate (animal units per area per a specific period of time) of pastures. This challenge represents a tremendous opportunity to leverage precision technology to account for individual animal variation in BW and growth, with subsequent impacts on herd-level decisions. Therefore, the objective of this study was to utilize precision livestock technology (PLT) collected data to build a precision system model (PSM) to evaluate the differences in predicted DMI using either initial BW, expected midseason BW, or PLT measured BW. The PSM model was built utilizing BW data measured using SmartScale (C-Lock Inc.) for 60 days during the summers of 2021 and 2022 on Angus yearling steers (average initial BW 393.71 ± 39.01 and 315.23 ± 53.91 kg, n = 130 and 124, for year 2021 and 2022, respectively) on native pastures at the South Dakota State University Cottonwood Field Station. The PSM evaluated total forage consumption and deterministically estimated hectares of pasture required to meet the herd forage demands relative to available biomass (kg/ha). the PSM estimated 4.49% and 6.94% more DMI at the herd level compared with using initial BW for years 2021 and 22, respectively, and 1.64% less DMI than mid-season BW in 2021. This resulted in an additional 14.03 and 17.95 ha required for years 2021 and 22, respectively, according to PSM estimates compared with initial BW, while animals were understocked by 5 and 0.3 ha for 2021 and 2022, respectively, using mid-season BW. Individuals expressed a divergent growth rate, resulting in greater misalignment between static and PSM predicted DMI as the grazing period progressed (Menendez et al., 2023), indicating greater opportunities for targeted grazing management in long grazing periods. Therefore, applying precision data provides more precise DMI estimates and demonstrates the advantages and disadvantages of herd-level estimates. The use of PSM helps to identify high-leverage precision tools to minimize a performance gap like overgrazing extensive rangeland systems and demonstrates the critical need to develop robust data-collection and processing steps to leverage continuously collected PLT data. [ABSTRACT FROM AUTHOR]

Attaining sustainability of livestock production and understanding environmental synergies requires in-depth knowledge of grazing animal growth and behavior. A pilot study (2021-22) was conducted at the South Dakota State University Cottonwood Field Station as part of a broader project to evaluate precision livestock technology and environmental synergies on native rangelands. Yearling Angus steers (n = 262) were fitted with virtual fencing collars (Vence), weighed on a traditional chute scale, and allocated to one of six native grass pastures equipped with individual weighing (SmartScale) scales at the water source. Each pasture was assigned either Rotational (RG) or Continuous (CG) grazing strategy and one of three stocking rates (Low, Medium, and High, 0.3, 0.42, and 0.7 AUMs, respectively) in a 2x3 factorial design. The data were downloaded, (Rcore Team, 2023), and spurious weights identified and removed using Robust Regression (hwts > 0.99, Parsons et al. 2023). Effects of stocking rate and grazing strategy were analyzed on water visits, time spent drinking, and growth using a linear mixed effects model to calculate effect sizes. Differences between BW and ADG calculated using the smart-scale vs. traditional chute weights were evaluated using a paired t-test. Steers visited the smart-scale 3.44 ± 2.79 visits per day, with significant effect of RG vs. CG strategies (3.65 ± 0.09 vs. 2.89 ± 0.10 visits per day respectively, P < 0.05). Total water visit duration averaged 5.04 ± 7.58 minutes per day, with most visits occurring between 0600 and 2000 hours and no observations occurring between 2000 and 0000 hours. RG managed steers exhibited significantly higher water visit duration (5.28 ± 0.308 vs 4.16 ± 0.317 minutes/day, P < 0.05) compared with CG managed steers. Smartscale measured weights were significantly heavier than chute weights at the beginning and end of the study period, (7.47 ± 24.54 and 9.77 ± 46.25 kg respectively, P < 0.05). No differences in overall ADG were found between smartscale and traditional chute weights(P > 0.58), however, significant temporal interactions occurred (P < 0.05), which demonstrates that ADG varied over the trial duration. This created significant discrepancies in predicted vs. actual stocking rates in heavy stocked pastures (0.84 AUM, P < 0.05), indicating pastures exceeded their target stocking rate. Overall, we found steers readily acclimated to smart-scale weighing systems, and creates a viable technology to monitor watering behavior, real-time body weight, and ADG in extensively managed cattle. Further, we showed RG vs CG grazing strategies significantly affect animal drinking behavior, while stocking rate resulted in inequalities between expected vs. actual assigned animal unit months. Precision livestock technologies offer a vital solution towards enhancing sustainable livestock management practices and improving nutrition and modeling in extensive rangeland systems. [ABSTRACT FROM AUTHOR]

Energy budgets in grazing cattle are notoriously difficult to study given complex environmental and physiological interactions between plants and animals, each operating according to individual grazing budgets. However, these synergistic energy pathways can be modeled using a series of mechanistic relationships describing forage resource distribution and animal behavior, each which can be observed with high levels of accuracy and precision using biometric sensors and remote sensing technology. The objective of this study is to demonstrate the coordination of data inputs into a coherent framework utilizing biometric and remote sensing technology. Crossbred steers (n = 10) were fitted with GPS and video tracking collars (Vectronic) and accelerometers (Wildbytes technologies), then managed for 286 days as part of a broader research grazing ecology project on pasture (~11.54 hectare) comprised of bermudagrass (Cynodon dactylon) and tall fescue (Festuca arundinacea) and equipped with an automated walk-over-weigh (TruTest) scale system. Distribution of forage quality was sampled monthly with a ½ m² quadrat using a 20 x 20-meter grid (n = 297) and remotely assessed via an unmanned aerial vehicle (Matrice 100) mounted hyperspectral camera (MicaSense) at 8 cm resolution. Animal behavior was predicted utilizing a trained randomforest prediction algorithm, and linked to geo-location and forage quality at the spatial-temporal scale in Program R. We then constructed animal ethogram, distribution of forage quality, and spatial-temporal distribution of animal behavior across the grazing landscape for a test period. We found steers allocated the greatest proportion of time to grazing and resting behaviors, 30 and 60% respectively, with the remainder dedicated to ruminating and walking behaviors. A diurnal, circadian rhythm in animal behavior demonstrates that steers grazed between 0900 and 1000 in the morning, and again between 1300 and 2000 in the evening, while resting behavior dominated the hours between 0300 and 0900, with a slight increase in grazing behavior noted at 0600, or approximately sunrise. Animal behavior linked forage availability showed steers selectively grazed areas with higher NDVI signatures, while seeking sources of water and cover to practice resting behavior. Given the need to develop robust models predicting forage intake and energy in grazing animals to improve sustainability and environmental metrics, animal tracking and biometric technologies offer a vital solution to improving management of extensively managed grazing livestock. [ABSTRACT FROM AUTHOR]

Animal behavior plays a crucial role as an indicator of animal health and nutritional status and serves as an indicator of animal growth. The objective of this study was to build an ethogram describing behavior in grazing cattle. We collected video and accelerometer data from crossbred steers (n = 10) used as part of a larger grazing study on the HH Leveck Animal Research Center, Mississippi State, MS. Daily Diary accelerometers (Wildbyte® technologies, Swansea) were programmed to collect magnetometer and accelerometer data at 40 Hz and attached to the GPS collars fitted on the animals prior to their release into a 10-hectare pasture of Tall Fescue and Bermudagrass, overseeded with Annual Ryegrass. Automated camera traps (Bushnell Essential®) were synced with UTC time and programmed to record 30-second video clips when triggered. Approximately 387,000 accelerometer signals representing 161 minutes of behavior from 10 animals were recorded, and behavior classified according to 1 of 5 categories: traveling, foraging, resting, ruminating, and grooming (Kilgour et al., 2012). Categorized accelerometer data was used to train a random forest model (Liaw and Weiner, 2002) in Program R (R Core Team, 2020), which resulted in a model sensitivity of 0.97, 0.93, 0.90, 0.87, and 0.80 for Traveling, Foraging, Resting, Ruminating, and Grooming, respectively, and an overall model accuracy of 0.95. Behaviors were aggregated into behavior bouts, and a daily ethogram was calculated for March 2019. This revealed that the steers spent the most amount of time traveling, an average of 1,026 minutes per day. This behavior was followed, in the average length of time, by foraging and resting for 205 ± 52.8 minutes and 31.8 ± 28.2 minutes per day, respectively. These results indicate the ability to accurately build a behavioral ethogram for grazing cattle and warrant further study in future research and livestock management. [ABSTRACT FROM AUTHOR]

Body weight (BW) is a critical component for monitoring animal weight gain, body condition, nutritional status. Remote animal weighing systems facilitate frequent collection of animal BW, however, datasets often contain spurious data. The objective of this study was to describe the utility of using a remote Walk-over-Weigh system and subsequent methods for data cleaning. Beef steers (n = 10) were tagged with Electronic RFID tags (EID) in an improved pasture (~12.1 hectares) containing Bermuda and Tall Fescue and inter-seeded with Annual Ryegrass and grazed from Feb. - Dec. 2020. Static chute weights (n = 80) were collected monthly, and a WOW system placed by the water to remotely collect BW (n = 5,466). Data were first loaded into Program R and scanned for spurious data using each of 2 primary approaches, 1) the whole herd and individual means ± 1 standard deviation (SD) calculated daily or over the entire trial and 2) each of 3 data smoothing algorithms, which included a quadratic growth model, cubic splines, and polynomial regression. Then, data with spurious observations removed were paired with static chute weights and fitted to a linear model to measure accuracy (mean bias) and precision (R2) of WOW data. Whole herd mean ± 1SD and individual daily mean ± 1SD identified 1,204 and 1,516 spurious data, with mean bias of -12.46 and -15.37 KG and R2 of 0.90 and 0.68, respectively. Smoothing functions identified 1,707, 4,684, and 4,776 spurious points, with a mean bias of 13.61, -19.78, and 12.58 KG, and R2 of 0.94, 0.70, and 0.87 for quadratic growth models, cubic splines, and polynomial regression, respectively. These results indicate the utility of using a simple WOW system to collect data for measuring growth curves and using weight data in a real-time fashion to make management and marketing decisions. [ABSTRACT FROM AUTHOR]

Previous research in feedlot studies has demonstrated that cattle feeding behavior is driven by internal metabolic processes and external environmental stimuli and serves as an indicator of animal health, nutritional status, and growth and feed quality and availability. However, technology has only recently allowed measurement of foraging behavior in grazing cattle. Objectives of this study were to measure frequency and duration of foraging bouts, meals, and total distance traveled during meals in grazing steers. The study was conducted as part of a larger grazing study on a 10-hectare Bermudagrass and Tall Fescue pasture, overseeded with Annual Ryegrass, located at the HH Leveck Animal Research Center, Mississippi State, MS. Using tri-axial accelerometers and GPS information from 10 crossbred steers, we examined foraging and meal bout frequency and duration and distance and speed traveled per meal for the period of March 2019. Observed animal behavior was used to train a randomforest model to predict foraging behavior, with model accuracy and sensitivity of 0.95 and 0.93, respectively. We found individual foraging bouts occurred on average 2,849 bouts per day and took on average 5.0 ± 1.8 min (range: 3-9 min), and that steers fed on average 205 ± 52.8 min/day (range: 120-270 min/day). Steers had an average of 9.5 ± 2.9 meals/day, that took on average, 89.3 ± 93.9 min/meal (range: 0.5-938.5 min/meal). Steers traveled an average of 412.4 ± 93.9 meters per meal, with an average foraging speed between 0 and 0.63 m/s. Traveling distance while foraging was positively correlated with meal length (0.83, P < 0.01) and foraging speed (0.70, P < 0.01). These results show that cattle grazing behavior can be accurately quantified in grazing cattle and warrants further research to examine associations between animal efficiency and performance, forage quality, and pasture management. [ABSTRACT FROM AUTHOR]