1. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
- Author
-
Malik Peiris, Gabriel Goderski, Lisa Wijsman, Joanna Ellis, Jean Louis Romette, Olfert Landt, Chantal B.E.M. Reusken, Marie Luisa Schmidt, Bas van der Veer, Adam Meijer, Christian Drosten, Julia Schneider, Herman Goossens, Maria Zambon, Marco Kaiser, Richard Molenkamp, Marion Koopmans, Daniel K.W. Chu, Daphne G.J.C. Mulders, Tobias Bleicker, Bart L. Haagmans, Sharon van den Brink, Victor M. Corman, Sebastian Brünink, and Virology
- Subjects
0301 basic medicine ,Epidemiology ,Computer science ,medicine.disease_cause ,0302 clinical medicine ,Global health ,diagnostics ,030212 general & internal medicine ,COVID-19 ,RT-PCR ,Wuhan ,novel coronavirus ,2019-nCoV ,laboratory ,testing ,Coronavirus ,outbreak ,media_common ,Reverse Transcriptase Polymerase Chain Reaction ,European research ,3. Good health ,PT-PCR ,Severe acute respiratory syndrome-related coronavirus ,Coronavirus Infections ,medicine.medical_specialty ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Pneumonia, Viral ,India ,03 medical and health sciences ,Betacoronavirus ,Virology ,medicine ,media_common.cataloged_instance ,European union ,Pandemics ,Clinical Laboratory Techniques ,SARS-CoV-2 ,Public health ,Research ,Public Health, Environmental and Occupational Health ,Outbreak ,Data science ,030104 developmental biology ,Workflow ,Coronavirus SARS-CoV-2 ,RNA ,Human medicine - Abstract
Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. Aim We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. Methods Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. Results The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project. Conclusion The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
- Published
- 2020
- Full Text
- View/download PDF