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Air pollution represented by particulate matter 2.5 (PM2.5) is closely related to diseases of the respiratory system. Although the understanding of its mechanism is limited, pulmonary inflammation is closely correlated with PM2.5-mediated lung injury. Soluble epoxide hydrolase (sEH) and epoxy fatty acids play a vital role in the inflammation. Herein, we attempted to use the metabolomics of oxidized lipids for analyzing the relationship of oxylipins with lung injury in a PM2.5-mediated mouse model, and found that the cytochrome P450 oxidases/sEH mediated metabolic pathway was involved in lung injury. Furthermore, the sEH overexpression was revealed in lung injury mice. Interestingly, sEH genetic deletion or the selective sEH inhibitor TPPU increased levels of epoxyeicosatrienoic acids (EETs) in lung injury mice, and inactivated pulmonary macrophages based on the MAPK/NF-κB pathway, resulting in protection against PM2.5-mediated lung injury. Additionally, a natural sEH inhibitor luteolin from Inula japonica displayed a pulmonary protective effect towards lung injury mediated by PM2.5 as well. Our results are consistent with the sEH message and protein being both a marker and mechanism for PM2.5-induced inflammation, which suggest its potential as a pharmaceutical target for treating diseases of the respiratory system.

This article addresses the issue of atmospheric pollution caused by solid particles in urban environments. The presence of PM10 and PM2,5 particles in the air of major cities and industrial areas worldwide has been examined. An evaluation of atmospheric pollution levels with PM10 and PM2,5 particles in Kostopil, considering current air quality standards in Ukraine and the European Union, has been conducted. The authors employed the gravimetric method to measure the levels of suspended dust particles (PM10 and PM2,5) in Kostopil from autumn 2022 to winter 2023. The study revealed an excessive amount of fine dust particles in the city's air, exceeding the maximum permissible values outlined in regulatory laws by 2.1-2.7 times. Furthermore, the monitoring of changes in suspended dust particle levels showed peak values of PM10 = 1.15 mg/m³ in January and PM2,5 = 0.96 mg/m³ in December. The results of the statistical analysis of particle level distribution in Kostopil's urban areas indicated the statistical significance of certain distribution parameters, specifically SW-W and D for PM10 and PM2,5 particle classes.

BackgroundWhile strong evidence supports adverse maternal and offspring consequences of air pollution, mechanisms that involve the placenta, a key part of the intrauterine environment, are largely unknown. Previous studies of air pollution and placental gene expression were small candidate gene studies that rarely considered prenatal windows of exposure or the potential role of offspring sex. We examined overall and sex-specific associations of prenatal exposure to fine particulate matter (PM2.5) with genome-wide placental gene expression.MethodsParticipants with placenta samples, collected at birth, and childhood health outcomes from CANDLE (Memphis, TN) (n = 776) and GAPPS (Seattle, WA) (n = 205) cohorts of the ECHO-PATHWAYS Consortium were included in this study. PM2.5 exposures during trimesters 1, 2, 3, and the first and last months of pregnancy, were estimated using a spatiotemporal model. Cohort-specific linear adjusted models were fit for each exposure window and expression of >11,000 protein coding genes from paired end RNA sequencing data. Models with interaction terms were used to examine PM2.5-offspring sex interactions. False discovery rate (FDR

This paper makes a theoretical contribution by exploring the integration of sustainability principles into project management. It compares two recent methodologies, PM2 and PRiSMTM, and provides a practical contribution by advocating for the adoption of these sustainability-driven practices among practitioners. Sustainability remains relatively unknown in the realm of project management, and this study aims to bridge that gap. This study follows an interpretivist philosophy and employs a combination of interviews and archival data analysis. PM2, created by the European Commission, provides a project management methodology free to organizations and includes best practices from other bodies of knowledge. However, it does not include sustainability because it aims to be generic. PRiSMTM, based on the P5™, aims to make the project management process more sustainable. PRiSMTM has a P5 Impact Analysis and Sustainability Management Plan as its main differentiating deliverables and is an extension of the Triple Bottom Line, also including product and process. The PM2 Alliance CEO believes that PM2 aims to be generic, so a focus on sustainability would remove the methodology's "elasticity". However, users wishing to use PM2 and consider sustainability can include it in the additional objectives and use PRiSMTM to differentiate deliverables. [ABSTRACT FROM AUTHOR]

PM2.5 is an air pollutant that is widely associated with adverse health effects, and which tends to be disproportionately located near low-income communities and communities of color. We applied a community-engaged research approach to assess the distribution of PM2.5 concentrations in the context of community concerns and urban features within and around the city of Santa Ana, CA. Approximately 183 h of one-minute average PM2.5 measurements, along with high-resolution geographic coordinate measurements, were collected by volunteer community participants using roughly two dozen low-cost AtmoTube Pro air pollution sensors paired with real-time GPS tracking devices. PM2.5 varied by region, time of day, and month. In general, concentrations were higher near the city's industrial corridor, which is an area of concern to local community members. While the freeway systems were shown to correlate with some degree of elevated air pollution, two of four sampling days demonstrated little to no visible association with freeway traffic. Concentrations tended to be higher within socioeconomically disadvantaged communities compared to other areas. This pilot study demonstrates the utility of using low-cost air pollution sensors for the application of community-engaged study designs that leverage community knowledge, enable high-density air monitoring, and facilitate greater health-related awareness, education, and empowerment among communities. The mobile air-monitoring approach used in this study, and its application to characterize the ambient air quality within a defined geographic region, is in contrast to other community-engaged studies, which employ fixed-site monitoring and/or focus on personal exposure. The findings from this study underscore the existence of environmental health inequities that persist in urban areas today, which can help to inform policy decisions related to health equity, future urban planning, and community access to resources.

BackgroundFine particulate matter (PM2.5) exposure accelerates atherosclerosis and contains known ovotoxic chemicals. However, effects of exposure to PM2.5 on the finite ovarian follicle pool have hardly been investigated, nor have interactions between ovarian and cardiovascular effects. We hypothesized that subchronic inhalation exposure to human-relevant concentrations of PM2.5 results in destruction of ovarian follicles via apoptosis induction, as well as accelerated recruitment of primordial follicles into the growing pool. Further, we hypothesized that destruction of ovarian follicles enhances the adverse cardiovascular effects of PM2.5 in females.ResultsHyperlipidemic apolipoprotein E (Apoe) null ovary-intact or ovariectomized female mice and testis-intact male mice were exposed to concentrated ambient PM2.5 or filtered air for 12 weeks, 5 days/week for 4 h/day using a versatile aerosol concentration enrichment system. Primordial, primary, and secondary ovarian follicle numbers were decreased by 45%, 40%, and 17%, respectively, in PM2.5-exposed ovary-intact mice compared to controls (P

BackgroundPrior studies examining the association between ambient air pollutants and pancreatic cancer have been conducted in racially/ethnically homogeneous samples and have produced mixed results, with some studies supporting evidence of an association with fine particulate matter.MethodsTo further investigate these findings, we estimated exposure levels of particulate matter (PM2.5, PM10) and oxides of nitrogen (NOX, and NO2) using kriging interpolation for 100,527 men and women from the Multiethnic Cohort Study, residing largely in Los Angeles County from 1993 through 2013. We measured the association between these air pollutants and incident pancreatic cancer using Cox proportional hazards models with time-varying pollutant measures, with adjustment for confounding factors.ResultsA total of 821 incident pancreatic cancer and 1,660,488 person-years accumulated over the study period, with an average follow-up time of over 16 years. PM2.5 (per 10 μg/m3) was associated with incident pancreatic cancer (hazard ratio [HR] = 1.61; 95% CI, 1.09, 2.37). This PM2.5 -association was strongest among Latinos (HR = 3.59; 95% CI, 1.60, 8.06) and ever smokers (HR = 1.76; 95% CI, 1.05, 2.94). There was no association for PM10 (HR = 1.12; 95% CI, 0.94, 1.32, per 10 μg/m3), NOx (HR = 1.14; 95% CI, 0.88, 1.48, per 50 ppb), or NO2 (HR = 1.14; 95% CI, 0.85, 1.54, per 20 ppb).ConclusionsOur findings support prior research identifying an association between fine particulate matter, PM2.5, and pancreatic cancer. Although not statistically heterogeneous, this association was most notable among Latinos and smokers. Future studies are needed to replicate these results in an urban setting and in a racially/ethnically diverse population.

ObjectiveTo characterize the association between residential environmental manganese (Mn) exposure and depression and anxiety, given prior associations among occupationally-exposed workers.MethodsWe administered the Beck Depression Inventory (BDI) and the State-Trait Anxiety Inventory (STAI) to 697 study participants in their preferred languages. These participants represented a population-based sample of residents aged ≥40 from two predominantly Black African communities in Gauteng province, South Africa: 605 in Meyerton, adjacent to a large Mn smelter, and 92 in Ethembalethu, a comparable non-exposed community. We investigated the associations between community (Meyerton vs. Ethembalethu) and severity of depression and anxiety, using linear regression, adjusting for age and sex. To document community-level differences in Mn exposure, we measured airborne PM2.5-Mn.ResultsMeyerton residents had BDI scores 5.63 points (95 % CI 3.07, 8.20) higher than Ethembalethu residents, with all questions contributing to this significant difference. STAI-state scores were marginally higher in Meyerton than Ethembalethu residents [2.12 (95 % CI -0.17, 4.41)], whereas STAI-trait scores were more similar between the communities [1.26 (95 % CI -0.82, 3.35)]. Mean PM2.5-Mn concentration was 203 ng/m3 at a long-term fixed site in Meyerton and 10 ng/m3 in Ethembalethu.ConclusionResidence near Mn emission sources may be associated with greater depression symptomatology, and possibly current, but not lifetime, anxiety.

European winter wheat cultivar "Tabasco" was reported to have resistance to powdery mildew disease caused by Blumeria graminis f. sp. tritici (Bgt) in China. In previous studies, Tabasco was reported to have the resistance gene designated as Pm48 on the short arm of chromosome 5D when a mapping population was phenotyped with pathogen isolate Bgt19 collected in China and was genotyped with simple sequence repeat (SSR) markers. In this study, single-nucleotide polymorphism (SNP) chips were used to rapidly determine the resistance gene by mapping a new F2 population that was developed from Tabasco and a susceptible cultivar "Ningmaizi119" and inoculated with pathogen isolate NCF-D-1–1 that was collected in the USA. The segregation of resistance in the population was found to link with Pm2 which was identified in Tabasco. Therefore, it was concluded that the previously reported Pm48 on chromosome arm 5DS in Tabasco should be the Pm2 gene on the same chromosome. The Pm2 was also found in European cultivars "Mattis" and "Claire" but not in any of the accessions from diploid wheat Aegilops tauschii or modern cultivars such as "Gallagher," "Smith's Gold," and "OK Corral" being used in the Great Plains in the USA. A KASP marker was developed to track the resistance allele Pm2 in wheat breeding. [ABSTRACT FROM AUTHOR]

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Background: Most studies on air pollution (AP) exposure have focused on adverse health effects of particulate matter (PM). Less well-studied are the actions of volatile organic compounds (VOCs) not retained in PM collections. These studies quantified chemical and biological properties of both PM2.5 and VOCs. Methods: Samples were collected near the Port of Los Angeles (Long Beach, LB), railroads (Commerce, CM), and a pollution-trapping topography-site (San Bernardino, SB). Quantitative assays were conducted: (1) chemical—prooxidant and electrophile content, (2) biological—tumor necrosis factor-α (TNF-α) and heme oxygenase-1 (HO-1) expression (3), VOC modulation of PM effects and (4), activation of the antioxidant response element (ARE) using murine RAW 264.7 macrophages. Results: SB site samples were the most potent in the chemical and biological assays, followed by a CM railroad site. Only PM2.5 exhibited significant proinflammatory responses. VOCs were more potent than PM2.5 in generating anti-inflammatory responses; further, VOC pretreatment reduced PM-associated TNF-α expression. VOCs significantly increased ARE activation compared to their corresponding PM2.5 which remained at background levels. Conclusion: Ambient VOCs are major contributors to adaptive responses that can modulate PM effects, in vitro, and, as such, need to be included in comprehensive assessments of AP.

We present an approach to analyzing fine particulate matter (PM2.5) data from a network of "low cost air quality monitors" (LCAQM) to obtain a finely resolved concentration map. In the approach, based on a dispersion model, we first identify the probable locations of the sources, and then estimate the magnitudes of the emissions from these sources by fitting model estimates of concentrations to corresponding measurements. The emissions are then used to estimate concentrations on a grid covering the domain of interest. The residuals between model estimates at the monitor locations and the measured concentrations are then interpolated to the grid points using Kriging. We illustrate this approach by applying it to a network of 20 LCAQMs located in the Imperial Valley of Southern California. Estimating the underlying mean concentration field with a dispersion model provides a more realistic estimate of the spatial distribution of PM2.5 concentrations than that from the Kriging observations directly.

Introduction: Using the ACMG-AMP guidelines for the interpretation of sequence variants, it remains difficult to meet the criterion associated with the protein domain, PM1, which is assigned in only about 10% of cases, whereas the criteria related to variant frequency, PM2/BA1/BS1, is reported in 50% of cases. To improve the classification of human missense variants using protein domains information, we developed the DOLPHIN system (https://dolphin.mmg-gbit.eu).Methods: We used Pfam alignments of eukaryotes to define DOLPHIN scores to identify protein domain residues and variants that have a significant impact. In parallel, we enriched gnomAD variants frequencies for each domains’ residue. These were validated using ClinVar data.Results: We applied this method to all potential human transcripts’ variants, resulting in 30.0% being assigned a PM1 label, whereas 33.2% were eligible for a new benign support criterion, BP8. We also showed that DOLPHIN provides an extrapolated frequency for 31.8% of the variants, compared to the original frequency available in gnomAD for 7.6% of them.Discussion: Overall, DOLPHIN allows a simplified use of the PM1 criterion, an expanded application of the PM2/BS1 criteria and the creation of a new BP8 criterion. DOLPHIN could facilitate the classification of amino acid substitutions in protein domains that cover nearly 40% of proteins and represent the sites of most pathogenic variants.

Pollution due to particulate matter can harm human health and the environment. One of the particulate matter sources is the mining industry. Depending on the type of activity (drilling, blasting, loading, hauling, dozing, crushing, screening, etc.), various levels of PM emission occurs in surface mining operations. For this reason, it is important to measure the PM emission values of each ongoing activity, evaluate them by taking into consideration the limit values specified in the relevant legislation and to plan preventive/mitigating measures. When the studies conducted on the subject were examined, it was found out that the equations modeling the PM emission, which may occur according to the type of activity carried out in surface mines, were developed but they varied even for the same activity among different mines. For this reason, it was concluded that PM emission values are specific to the surface mines, that continuous emission measurement will provide more accurate results, and therefore, as the most realistic approach, separate PM emission modeling should be done for each activity carried out in a surface mine.

Hanoi and Ho Chi Minh City (HCM), the most populous cities in Vietnam, have received increasing global attention because of their poor air pollution status. As part of the recent UK-Vietnam 2-Cities project, the concentrations of trace metals in fine particulate matter have been characterized. 24-hour samples of PM2 were collected at 2 sites in Hanoi and 3 sites in HCM during two 4-week periods in September/October 2018 and March 2019. The soluble fraction of 15 trace metal(oid)s (Fe, Al, Mn, Ti, Zn, V, Cu, Ni, Co, Cd, Pb, Th, Cr, As, and Sb) bound to PM2 were analyzed by ICP-MS. The results show that Zn was the most abundant soluble metal in PM2 in both cities, with very large numbers of road vehicles (e.g. tyre wear) likely contributing in both cities and non-ferrous metal production being a substantial additional source in Hanoi. Fe and Al, derived from crustal sources, were the dominant metals after Zn. Most trace metals concentrations in Hanoi were higher than in HCM, especially toxic metals such as Pb, Cd, Cr and As. V and Ni were the only two metals having higher concentrations in HCM than in Hanoi, likely due to shipping emissions (combustion of heavy fuel oil) that strongly affect the air quality in HCM. Coal-power plants and non-ferrous metal production are likely to be the major sources of trace metals in Hanoi. Health risk assessment shows that a high carcinogenic risk exists for inhalation exposure of soluble trace metals bound to PM2 in both cities.

Powdery mildew of wheat, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that seriously reduces yield and quality worldwide. Utilization of plant resistance genes is an attractive and effective strategy for controlling this disease. Among the reported powdery mildew (Pm) resistance genes, Pm2 exhibits a diverse resistance spectrum among its multiple alleles. It has been widely used in China for resistance breeding for powdery mildew. To mine more Pm2 alleles and clarify their distribution, we screened 33 wheat cultivars/breeding lines carrying Pm2 alleles from 641 wheat genotypes using diagnostic and Pm2 -linked markers. To further investigate the relationships within the Pm2 alleles, we compared their resistance spectra, polymorphism of marker alleles and gene sequences, and found that they have identical marker alleles and gene sequences but diverse resistance spectra. In addition, the diagnostic kompetitive allele-specific PCR (KASP) marker, YTU-KASP-Pm2 , was developed and was shown to detect all the Pm2 alleles in the different genetic backgrounds. These findings provide valuable information for the distribution and rational use of Pm2 alleles, push forward their marker-assisted breeding (MAS), and hence improve the control of wheat powdery mildew. [ABSTRACT FROM AUTHOR]

Powdery mildew of wheat, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that seriously reduces yield and quality worldwide. Utilization of plant resistance genes is an attractive and effective strategy for controlling this disease. Among the reported powdery mildew (Pm) resistance genes, Pm2 exhibits a diverse resistance spectrum among its multiple alleles. It has been widely used in China for resistance breeding for powdery mildew. To mine more Pm2 alleles and clarify their distribution, we screened 33 wheat cultivars/breeding lines carrying Pm2 alleles from 641 wheat genotypes using diagnostic and Pm2-linked markers. To further investigate the relationships within the Pm2 alleles, we compared their resistance spectra, polymorphism of marker alleles and gene sequences, and found that they have identical marker alleles and gene sequences but diverse resistance spectra. In addition, the diagnostic kompetitive allele-specific PCR (KASP) marker, YTU-KASP-Pm2, was developed and was shown to detect all the Pm2 alleles in the different genetic backgrounds. These findings provide valuable information for the distribution and rational use of Pm2 alleles, push forward their marker-assisted breeding (MAS), and hence improve the control of wheat powdery mildew.

Purpose: We suggest using a project management approach to manage sports events. To this aim, we propose an adaptation of PM², the project management methodology developed inside the European Commission, to manage sports projects. Design/methodology/approach: We perform theoretical analyses arguing why a sport event is a project, about the roles of the event and sport management and a theorethical adaptation of the PM2 methodology to sport management. Findings: We show that events management could benefit from PM² methodology if we introduce several adaptations. The main one implies splitting the original project execution phase into two separate ones: the deployment phase and the execution (strito sensu). The former includes all the activities performed to prepare for the sports competition; the last, to cope with the complexity of the competition particular dates, characterized by simultaneous and mass involvement of participants and spectators in the event. We also propose changes into the original project governance model, for accommodating a market orientation to maximize the economic return of the event and for including the concept of “extended customer” that comprises the owner of the event, the participants and the spectators. Research limitations/implications: Further empirical research is needed to analyze the implementation of this adaptation of PM² . Practical implications: We offer an adaptation of PM² methodology for the event managers that allow them to apply project management standards in their particular events. Our adaptation accommodates the marketing approach and the market orientation commonly used by event managers. Social implications: The redefinition of the role of sports event managers with a project management orientation allows to introduce the cost-benefit analysis in the development of a sport event, making sports events not only successful but also profitable, sustainable and efficient. Originality/value: For the first time, PM² is applied to events, in particular, to sport events. The specific characteristics of PM2 make it interesting in any sector, as sports events, where managers do not have extensive experience using project management standards and methodologies. [ABSTRACT FROM AUTHOR]

Powdery mildew induced by Blumeria graminis f. sp. Tritici (Bgt) has a devastating impact on global wheat yield and quality. Host resistance is the most effective and economical means to control this disease. In this study, Heng 4568, an elite wheat cultivar, shows high resistance to 12 Bgt isolates from different regions in China at the seedling stage. Genetic analysis demonstrates that the powdery mildew resistance in Heng 4568 is conferred by a single dominant locus, temporarily designated PmH4568. Furthermore, PmH4568 is mapped to the reported Pm2 interval on chromosome 5DS with five Pm2 linked markers and flanked by the markers Bwm20 and Bwm21 with a genetic distance of 0.3 and 0.6 cM, respectively. To further investigate the relationship between PmH4568 and Pm2 , the diagnostic marker Pm2b-map-3 of Pm2 is used to genotype the F2:3 population derived from the cross Heng 4568 × Daimai 2173. Notably, there is no recombination found, indicating that PmH4568 is also probably a Pm2 allele. In addition, five closely linked markers as well as one diagnostic marker are successfully developed and tested in 16 wheat cultivars from different agro-ecological areas in China, which have potential applications in molecular breeding by marker-assisted selection. [ABSTRACT FROM AUTHOR]

Powdery mildew induced by Blumeria graminis f. sp. Tritici (Bgt) has a devastating impact on global wheat yield and quality. Host resistance is the most effective and economical means to control this disease. In this study, Heng 4568, an elite wheat cultivar, shows high resistance to 12 Bgt isolates from different regions in China at the seedling stage. Genetic analysis demonstrates that the powdery mildew resistance in Heng 4568 is conferred by a single dominant locus, temporarily designated PmH4568. Furthermore, PmH4568 is mapped to the reported Pm2 interval on chromosome 5DS with five Pm2 linked markers and flanked by the markers Bwm20 and Bwm21 with a genetic distance of 0.3 and 0.6 cM, respectively. To further investigate the relationship between PmH4568 and Pm2, the diagnostic marker Pm2b-map-3 of Pm2 is used to genotype the F2:3 population derived from the cross Heng 4568 × Daimai 2173. Notably, there is no recombination found, indicating that PmH4568 is also probably a Pm2 allele. In addition, five closely linked markers as well as one diagnostic marker are successfully developed and tested in 16 wheat cultivars from different agro-ecological areas in China, which have potential applications in molecular breeding by marker-assisted selection.

Reliability growth models are commonly used in the Department of Defense (DoD) to plan, track, and project reliability during system acquisition and testing. We describe two commonly used classes of reliability growth models for continuous failure time data and the metrics appropriate for their use. We also present two Bayesian reliability growth models that are based on the DoD models. The Bayesian models are easily interpretable in a statistical framework, which supports estimation and uncertainty quantification. Our goal is to provide a practical understanding of the development, implementation, and use of reliability growth models across a sequence of DoD testing events. [ABSTRACT FROM AUTHOR]

Abstract Background Precision therapeutics continuously make advances in cancer therapy, and a field of growing interest is the combination of targeted radionuclide therapy (TRNT) with potential radiosensitizing agents. This study evaluated whether the effects of in vitro TRNT, using the 177Lu-labeled anti-CD44v6 antibody AbN44v6, were potentiated by the novel stapled MDM2/X-p53 antagonist PM2. Materials and methods Two wt p53 cell lines, HCT116 (colorectal carcinoma) and UM-SCC-74B (head and neck squamous cell carcinoma), expressing different levels of the target antigen, CD44v6, were used. Antigen-specific binding of 177Lu-AbN44v6 was initially verified in a 2D cell assay, after which the potential effects of unlabeled AbN44v6 on downstream phosphorylation of Erk1/2 were evaluated by western blotting. Further, the therapeutic effects of unlabeled AbN44v6, 177Lu-AbN44v6, PM2, or a combination (labeled/unlabeled AbN44v6 +/− PM2) were assessed in 3D multicellular tumor spheroid assays. Results Radiolabeled antibody bound specifically to CD44v6 on both cell lines. Unlabeled AbN44v6 binding did not induce downstream phosphorylation of Erk1/2 at any of the concentrations tested, and repeated treatments with the unlabeled antibody did not result in any spheroid growth inhibition. 177Lu-AbN44v6 impaired spheroid growth in a dose-dependent and antigen-dependent manner. A single modality treatment with 20 μM of PM2 significantly impaired spheroid growth in both spheroid models. Furthermore, the combination of TRNT and PM2-based therapy proved significantly more potent than either monotherapy. In HCT116 spheroids, this resulted in a two- and threefold spheroid growth rate decrease for the combination of PM2 and 100 kBq 177Lu-AbN44v6 compared to monotherapies 14-day post treatment. In UM-SCC-74B spheroids, the combination therapy resulted in a reduction in spheroid size compared to the initial spheroid size 10-day post treatment. Conclusion TRNT using 177Lu-AbN44v6 proved efficient in stalling spheroid growth in a dose-dependent and antigen-dependent manner, and PM2 treatment demonstrated a growth inhibitory effect as a monotherapy. Moreover, by combining TRNT with PM2-based therapy, therapeutic effects of TRNT were potentiated in a 3D multicellular tumor spheroid model. This proof-of-concept study exemplifies the strength and possibility of combining TRNT targeting CD44v6 with PM2-based therapy.

After literally half a century of studies conducted in the western countries on the levels of pollutants in the atmosphere and their effects on human health, it is scientifically confirmed that a low quality of air quality leads to a state of Poor people's health. Particulate particles are criminated to have the most severe negative impact on the health of the population. In Romania it was updated according to European requirements, legislation in the field, by the adoption by the Romanian Parliament of Law No. 104 of 15 June 2011 on ambient air quality. This normative act aims to ensure support for the protection of human health and the environment, as a whole, by regulating measures intended to preserve ambient air quality, where this corresponds to the objectives for the quality Ambient air set out in that link.

Present state of air quality is determined according to emission and imission situation of basic pollutants. According to obtained information about the state of air quality the development of imission and emission has at the present time decreasing tendency caused especially by industrial production innovations in European countries, with slight rises renewable energy sources for electricity production, but especially the effort to improve the condition relates to the use of mandatory quotas and pollution charges in the European Union. The aim of the this article is the review of emission development in the year 1998 up to 2013 for Central European countries - Slovakia, Czech Republic and Poland and their comparison to the European average value of released emission. As pollutants were evaluated NOx and particulate matter PM10, PM2,5, which in the last few years contaminate the air and in particular the main environment.

Introduction. The purpose of the article is to assess the quality of indoor air in university by measuring the concentration of suspended particles. In the Russian Federation, the rationing of maximum permissible concentrations of undifferentiated dust in atmospheric air depends on particle size.Materials and methods. The research carries out measurements of suspended particles concentrations in the air of six audiences in the Moscow State National Research University of Civil Engineering (NRU MGSU).Results. The obtained results of concentration measurements (particle/m3) are recalculates in mg/m3, and compared with threshold values of maximum one-time concentrations of suspended particles.Discussion and conclusions. In calm weather, air pollution by suspended particles in the surface layer practically does not affect the quality of internal microclimate, which is associated with significant decrease of dust concentration in height and insufficient air exchange in natural ventilation systems.

Se lleva a cabo un estudio de correlación estadística entre datos numéricos correspondientes a la concentración de las partículas suspendidas y a la concentración de gases contaminantes que son obtenidos de las mediciones registradas en la base de datos de la Red Automática de Monitoreo Atmosférico (RAMA) para la Ciudad de México y el Área Metropolitana. Se reporta una importante dependencia entre los pares de gases NO2-NOX, NO-NOX, CO-NO, CO-NOX y los pares de partículas PM2.5-PM10, PMCO-PM10 cuando el estudio se realiza tomando como referencia una sola estación de monitoreo. Por otra parte, se encuentra una estrecha relación entre el ozono (O3) de una estación con el de otra para diferentes pares de estaciones.

Background: Precision therapeutics continuously make advances in cancer therapy, and a field of growing interest is the combination of targeted radionuclide therapy (TRNT) with potential radiosensitizing agents. This study evaluated whether the effects of in vitro TRNT, using the 177Lu-labeled anti-CD44v6 antibody AbN44v6, were potentiated by the novel stapled MDM2/X-p53 antagonist PM2. Materials and methods: Two wt p53 cell lines, HCT116 (colorectal carcinoma) and UM-SCC-74B (head and neck squamous cell carcinoma), expressing different levels of the target antigen, CD44v6, were used. Antigen-specific binding of 177Lu-AbN44v6 was initially verified in a 2D cell assay, after which the potential effects of unlabeled AbN44v6 on downstream phosphorylation of Erk1/2 were evaluated by western blotting. Further, the therapeutic effects of unlabeled AbN44v6, 177Lu-AbN44v6, PM2, or a combination (labeled/unlabeled AbN44v6 +/− PM2) were assessed in 3D multicellular tumor spheroid assays. Results: Radiolabeled antibody bound specifically to CD44v6 on both cell lines. Unlabeled AbN44v6 binding did not induce downstream phosphorylation of Erk1/2 at any of the concentrations tested, and repeated treatments with the unlabeled antibody did not result in any spheroid growth inhibition. 177Lu-AbN44v6 impaired spheroid growth in a dose-dependent and antigen-dependent manner. A single modality treatment with 20 μM of PM2 significantly impaired spheroid growth in both spheroid models. Furthermore, the combination of TRNT and PM2-based therapy proved significantly more potent than either monotherapy. In HCT116 spheroids, this resulted in a two- and threefold spheroid growth rate decrease for the combination of PM2 and 100 kBq 177Lu-AbN44v6 compared to monotherapies 14-day post treatment. In UM-SCC-74B spheroids, the combination therapy resulted in a reduction in spheroid size compared to the initial spheroid size 10-day post treatment. Conclusion: TRNT using 177Lu-AbN44v6 proved efficient in stalling spheroid growth in a dose-dependent and antigen-dependent manner, and PM2 treatment demonstrated a growth inhibitory effect as a monotherapy. Moreover, by combining TRNT with PM2-based therapy, therapeutic effects of TRNT were potentiated in a 3D multicellular tumor spheroid model. This proof-of-concept study exemplifies the strength and possibility of combining TRNT targeting CD44v6 with PM2-based therapy. [ABSTRACT FROM AUTHOR]

W pracy przedstawiono statystyczną ocenę wpływu temperatury powietrza oraz inwersji termicznych (dolnych i górnych) na stężenia pyłu zawieszonego PM10 i PM2,5 oraz dwutlenku siarki w Gdańsku, Wrocławiu i Warszawie w okresie kalendarzowej zimy XII-II w 2016/17 r. Wykazano, że w analizowanym sezonie zimowym temperatura powietrza w przygruntowej warstwie powietrza (do 200 cm) wywierała silny wpływ na zmienność stężeń dwutlenku siarki i pyłu zawieszonego obu frakcji w każdym mieście, zwłaszcza w porze nocnej (19.00-6.00). Niekorzystny wpływ na wielkość analizowanych zanieczyszczeń miała przede wszystkim miąższość dolnych warstw inwersyjnych, podczas gdy zjawiskiem jednoznacznie korzystnym dla dyspersji zanieczyszczeń, była duża miąższość dziennej warstwy mieszania, uwarunkowana wysokim zaleganiem podstawy inwersji górnych.

This paper presents a case study on the influence of the energy efficiency of a steam boiler of 330 MW energy group on the environment. The Benson boiler works with powdered lignite. We present the results of experimental measurements on immission and emissions of pollutants resulting from burning lignite: SO2, NOx, PM10, PM2,5, TSP. Experimental measurements were performed on the boilers of 330MW power units of the thermoelectric plant of Rovinari.

The tumor suppressor p53 is a key mediator of cellular stress and DNA damage response cascades and is activated after exposure to ionizing radiation. Amplifying wild-type p53 expression by targeting negative regulators such as MDM2 in combination with external beam radiotherapy (EBRT) may result in increased therapeutic effects. The novel stapled peptide PM2 prevents MDM2 from suppressing wild-type p53, and is thus a promising agent for therapeutic combination with EBRT. Effects of PM2 and potential PM2-induced radiosensitivity were assessed in a panel of cancer cell lines using 2D cell viability assays. Western Blot and flow cytometric analyses were used to investigate the mechanisms behind the observed effects in samples treated with PM2 and EBRT. Finally, PM2-treatment combined with EBRT was evaluated in an in vitro 3D spheroid model. PM2-therapy decreased cell viability in wild-type p53, HPV-negative cell lines. Western Blotting and flow cytometry confirmed upregulation of p53, as well as initiation of p53-mediated apoptosis measured by increased cleaved caspase-3 and Noxa activity. Furthermore, 3D in vitro tumor spheroid experiments confirmed the superior effects of the combination, as the only treatment regime resulting in growth inhibition and complete spheroid disintegration. We conclude that PM2 induces antitumorigenic effects in wt p53 HPV-negative cancer cells and potentiates the effects of EBRT, ultimately resulting in tumor eradication in a 3D spheroid model. This strategy shows great potential as a new wt p53 specific tumor-targeting compound, and the combination of PM2 and EBRT could be a promising strategy to increase therapeutic effects and decrease adverse effects from radiotherapy.

The tumor suppressor p53 is a key mediator of cellular stress and DNA damage response cascades and is activated after exposure to ionizing radiation. Amplifying wild-type p53 expression by targeting negative regulators such as MDM2 in combination with external beam radiotherapy (EBRT) may result in increased therapeutic effects. The novel stapled peptide PM2 prevents MDM2 from suppressing wild-type p53, and is thus a promising agent for therapeutic combination with EBRT. Effects of PM2 and potential PM2-induced radiosensitivity were assessed in a panel of cancer cell lines using 2D cell viability assays. Western Blot and flow cytometric analyses were used to investigate the mechanisms behind the observed effects in samples treated with PM2 and EBRT. Finally, PM2-treatment combined with EBRT was evaluated in an in vitro 3D spheroid model. PM2-therapy decreased cell viability in wild-type p53, HPV-negative cell lines. Western Blotting and flow cytometry confirmed upregulation of p53, as well as initiation of p53-mediated apoptosis measured by increased cleaved caspase-3 and Noxa activity. Furthermore, 3D in vitro tumor spheroid experiments confirmed the superior effects of the combination, as the only treatment regime resulting in growth inhibition and complete spheroid disintegration. We conclude that PM2 induces antitumorigenic effects in wt p53 HPV-negative cancer cells and potentiates the effects of EBRT, ultimately resulting in tumor eradication in a 3D spheroid model. This strategy shows great potential as a new wt p53 specific tumor-targeting compound, and the combination of PM2 and EBRT could be a promising strategy to increase therapeutic effects and decrease adverse effects from radiotherapy. [ABSTRACT FROM AUTHOR]

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Las partículas atmosféricas constituyon uno de los contaminantes atmosféricos más importantes, debido a que contienen metales e hidrocarburos aromáticos policíclicos (HAP) que son compuestos con conocida actividad genotóxica, mutagénica y carcinogénica. Las partículas finas con diámetro menor a 2,5 µm (PM2,5) producidas por la combustión de los vehículos diésel están implicadas en efectos sobre la salud humana. El propósito de esta investigación fue determinar la actividad mutagénica y genotóxica de la fracción respirable del PM2,5, presente cerca de la avenida fronteriza de alto flujo vehicular en Villa de Rosario (Norte de Santander), la cual comunica los países de Colombia y Venezuela. El PM2,5 fue evaluado con filtros de cuarzo Pallflex. La actividad mutagénica y genotóxica de los extractos del PM2,5 fue determinada mediante la prueba de Ames y el ensayo cometa. En la prueba de Ames se utilizaron las cepas TA98 y TA100 de Salmonella typhimurium y el daño genotóxico se evaluó en linfocitos de sangre periférica. Los resultados mostraron mutagenicidad y genotoxicidad asociada con la fracción PM2,5 en Villa del Rosario, lo cual representa un riesgo en la aparición de enfermedades como el cáncer en la población expuesta.

El uso doméstico de leña representa una condición socioecológica con importantes efectos sobre la salud, principalmente en las zonas rurales de los países en desarrollo. El objetivo del estudio fue evaluar la exposición a hidrocarburos aromáticos policíclicos (HAP) en mujeres y niños, así como la concentración de PM2,5 en interiores de viviendas indígenas de Tocoy, San Antonio, San Luis Potosí, México. Con los resultados, se analizó la perspectiva de los padres para la toma de decisiones en la atención a la salud ambiental infantil por riesgos de exposición a tóxicos. Utilizamos el 1-hidroxipireno como un biomarcador de exposición a HAP y se evaluaron las PM2,5 de 24 horas en interiores de viviendas indígenas mediante equipos Minivol®. El 100 % de las viviendas estudiadas utilizaban biomasa para la cocción de sus alimentos y el 70 % quemaban basura en sus hogares. Se encontraron niveles de 1-OHP en mujeres y niños de 1,15 (0,28-2,1) y 1,04 (0,28-3,62) μmol/mol creatinina respectivamente. Además, se detectaron concentraciones medias de PM2,5 de 93,3 ± 3,6 μg/m3. Finalmente se mostró que el 90 % de los cuidadores indican que el niño se concentra en el área de la cocina y traspatio, existiendo poca o nula adaptación para la seguridad del niño. Esta comunidad indígena es un ejemplo de los riesgos en salud asociados a contaminación de aire de interiores en México; por consiguiente se requiere de programas de intervención dirigidos a reducir la exposición a estos tóxicos en poblaciones vulnerables, iniciando por la educación de los cuidadores.

Powdery mildew, caused by Blumeria graminis f. sp. tritici , is a devastating disease that seriously threatens wheat yield and quality. To control this disease, host resistance is the preferred measure. However, wheat breeding is a complex process with elusive exchange and recombination of the traits from their parents. Increased resistance often leads to a decline in other key traits, such as yield and quality. Developing breakthrough germplasms with harmonious powdery mildew resistance and other key breeding traits is attractive in wheat breeding. In this study, we developed an ideal wheat breeding line AL46 that pyramided its hexaploid triticale parent-derived desirable yield traits and its wheat parent-derived powdery mildew resistance gene Pm2 . Sequential genomic in situ hybridization (GISH), multicolor GISH, multicolor fluorescence in situ hybridization, and molecular marker analyses revealed that AL46 was a wheat-rye T1RS·1BL translocation line. Genetic analysis combined with function marker detection and sequence alignment were used to confirm that AL46 carried the Pm2 gene. Then, we evaluated the powdery mildew resistance and comprehensive traits of AL46, and just as we designed, AL46 showed harmonious powdery mildew resistance with some key breeding traits. This study not only developed an ideal wheat germplasm resource but also provided a successful example for pyramiding breeding, which could be a promising direction for wheat improvement in the future., Competing Interests: The author(s) declare no conflict of interest.

PM10 and PM2,5 were sampled simultaneously in urban and volcanic environments in the Metropolitan Area of Costa Rica from October to November 2012. Higher mass concentrations (42-29 μgm-3) of PM10 and PM2,5 were found at industrial and commercial areas with high traffic flow (La Uruca, Heredia and Belen) compared with those found in the volcanic areas. The daily concentrations of PM10 and PM2,5 obtained in the Poas Volcano ranged from 3 -14 μgm-3 and 2-11 μgm-3, respectively. However the acidity of the collected particles in the Poas volcano was higher than those sampled in urban environments probably due to a lower occurrence rate of neutralization. The contribution of secondary ions was more important to the volcanic PM10 composition (around 40%), unlike the PM10 collected in urban areas.