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Your search keyword '"Othman, Iekhsan"' showing total 400 results
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400 results on '"Othman, Iekhsan"'

15. Rosmarinic acid-rich ethanolic extract of Orthosiphon stamineus ameliorates cognitive and hippocampal long-term potentiation in chronic cerebral hypoperfusion rat model

Author

Hassan, Zurina, primary, Kumaran, KKesevan Rajah, additional, Chear, Nelson Jeng-Yeou, additional, Syuhadaa Bakar, Siti Najmi, additional, Retinasamy, Thaarvena, additional, Bhuvanendran, Saatheeyavaane, additional, Abdul Majeed, Amin Malik, additional, Shaikh, Mohd. Farooq, additional, and Othman, Iekhsan, additional

Published
2023
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25. Ensemble Adaptive Total Variation Graph Regularized NMF for Singlecell RNA-seq Data Analysis

Author

Tahir Ansari Mohammad, B. Kirmizibayrak Petek, El-Baz Ahmed, Shaldam Moataz, Mansour Basem, Yahya Galal, K. Kaymakcioglu Bedia, Tudu Praveen, Keuper Kristina, Gopalakrishnan Nair Gopakumar, Madhavan Manu, Bhuvanendran Saatheeyavaane, Kong Xiang-Zhen, Liu Jin-Xing, Othman Iekhsan, Zhu Ya-Li, Gao Ying-Lian, Sriramulu Sushmitha, Tok Fatih, Chaudhuri Punarbasu, Ning Chen Win, Pathak Surajit, Mahanty Shouvik, A. Sahar Esra, Farooq Shaikh Mohd., Zhu Rong, Kutty Radhakrishnan Ammu, Date Abhijit, and Yilmaz Sinem

Subjects
Computational Mathematics, Variation (linguistics), Genetics, Graph (abstract data type), RNA-Seq, Computational biology, Molecular Biology, Biochemistry, Mathematics, Non-negative matrix factorization
Abstract

Background: Single-cell RNA sequencing techniques have emerged as effective approaches for finding the heterogeneity between cells and discovering the differentiation stage. Adaptive total variation graph regularized nonnegative matrix factorization (ATV-NMF) has been proposed to capture the inner geometric structure and determine whether to retain feature details or denoise, which is suitable for analyzing single-cell data. However, the rank of matrix factorization significantly affects clustering performance greatly, and it is still challenging to determine the optimal rank. Objective: To solve the problem, in this paper, we propose an ensemble clustering method ANMFCE to integrate several base clustering results corresponding to different parameter rank values. Methods: Firstly, we use the ATV-NMF algorithm to obtain clustering results with different dimension reduction ranks. Secondly, the consensus function based on connected-triple-based similarity is applied to obtain the similarity matrix. Finally, the spectral clustering method is used to find the final optimal partition. Results: Clustering results on six single-cell sequencing datasets show that our method is more advanced than the individual ATV-NMF method and other comparison methods, which can illustrate that our method is effective in finding the heterogeneity in single-cell datasets. Moreover, the identification of gene markers also achieves accurate results. Conclusion: In summary, our method is effective for analyzing single-cell RNA sequencing datasets.

Published
2021

27. Estimating economic and disease burden of snakebite in ASEAN countries using a decision analytic model

Author

Patikorn, Chanthawat, primary, Blessmann, Jörg, additional, Nwe, Myat Thet, additional, Tiglao, Patrick Joseph G., additional, Vasaruchapong, Taksa, additional, Maharani, Tri, additional, Doan, Uyen Vy, additional, Zainal Abidin, Syafiq Asnawi, additional, Ismail, Ahmad Khaldun, additional, Othman, Iekhsan, additional, Taychakhoonavudh, Suthira, additional, and Chaiyakunapruk, Nathorn, additional

Published
2022
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29. Typical response of CD14++CD16– monocyte to knee synovial derived mediators as a key target to overcome the onset and progression of osteoarthritis

Author

Saffery, Nik Syazana Izyan, primary, Genasan, Krishnamurithy, additional, Chan, Chee Ken, additional, Ayob, Khairul Anwar, additional, Teo, Seow Hui, additional, Al-Fayyadh, Mohamed Zubair Mohamed, additional, Othman, Iekhsan, additional, Abidin, Syafiq Asnawi Zainal, additional, Raman, Murali Malliga, additional, Raghavendran, Hanumantha Rao Balaji, additional, and Kamarul, Tunku, additional

Published
2022
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31. Situation of snakebite, antivenom market and access to antivenoms in ASEAN countries

Author

Patikorn, Chanthawat, primary, Ismail, Ahmad Khaldun, additional, Abidin, Syafiq Asnawi Zainal, additional, Blanco, Francis Bonn, additional, Blessmann, Jörg, additional, Choumlivong, Khamla, additional, Comandante, John David, additional, Doan, Uyen Vy, additional, Mohamed @ Ismail, Zainalabidin, additional, Khine, Yi Yi, additional, Maharani, Tri, additional, Nwe, Myat Thet, additional, Qamruddin, Reza Murad, additional, Safferi, Ruth Sabrina, additional, Santamaria, Emelia, additional, Tiglao, Patrick Joseph G, additional, Trakulsrichai, Satariya, additional, Vasaruchapong, Taksa, additional, Chaiyakunapruk, Nathorn, additional, Taychakhoonavudh, Suthira, additional, and Othman, Iekhsan, additional

Published
2022
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33. Development of ATP13A2-deficient In vitro Model for PARK9 Parkinson’s Disease

Author

Jye Yap Yiing, Yen Ng Khuen, Moi Chye Soi, Gah Leong Voon Kenny, Yian Koh Rhun, Othman Iekhsan, and Hoque Chowdhury Ezharul

Subjects
Endocrinology, Parkinson's disease, Immunology, medicine, Pharmacology (medical), Biology, medicine.disease, In vitro model
Abstract

Background: PARK9 familial Parkinson’s disease (PD) is caused by a loss-of-function mutation in the ATP13A2 gene in which the mutation impairs the autophagic-lysosomal degradation pathway and induces intraneuronal accumulation of alpha-synuclein. RNA interference has been a useful tool in generating in vitro knockdown model to study the physiological role of the gene. However, the availability of a validated ATP13A2-deficient in vitro model is limited. Objective: This study aimed to develop the ATP13A2-deficient PD model by delivering ATP13A2 siRNA into neuroblastoma cells using carbonate apatite nanoparticles (CA NPs). Methods: CA NPs were fabricated using different concentrations of calcium chloride and characterised in the presence or absence of ATP13A2 siRNA. Time-dependent stabilities of CA NPs and CA NPs-associated siRNA (CA-siRNA) complex were evaluated by pH, turbidity, size, and zeta potential measurements. The dissolution abilities at acidic conditions of both complexes were investigated. Following that, green fluorescence protein (GFP) and four different siRNAs targeting ATP13A2 (siRNA_5, 6, 7, and 8) were transfected to cells with the fabricated CA NPs. Western blot was performed to determine the knockdown effect of the four siRNAs. Results: It was found that 4 mM calcium chloride was ideal for CA NP formation, while an incubation time of 48 hours was required to maintain the stability of nanoparticles. Successful transfection was confirmed by detection of fluorescence signal from the GFP plasmid and the subsequent silencing of this signal by transfecting GFP siRNA. Western blot analysis revealed that ATP13A2 protein expression was significantly reduced to 20% upon transfection with 20 nM of siRNA_5. Conclusion: ATP13A2-deficient PD model was successfully developed.

Published
2021

38. A Perspective on the Impact of COVID-19 Pandemic on Basic Science Research and its Future Implications

Author

Farooq Shaikh Mohd., Angelopoulou Efthalia, Raj Giri Bhupendra, Nath Paudel Yam, Othman Iekhsan, and Piperi Christina

Subjects
Microbiology (medical), Coronavirus disease 2019 (COVID-19), Immunology, Perspective (graphical), Pandemic, Immunology and Allergy, Engineering ethics, Sociology
Abstract

COVID-19 has emerged as a devastating pandemic of the century that the current generations have ever experienced. The COVID-19 pandemic has infected more than 12 million people around the globe, and 0.5 million people have succumbed to death. Due to the lack of effective vaccines against the COVID-19, several nations throughout the globe have imposed a lock-down as a preventive measure to lower the spread of COVID-19 infection. As a result of lock-down, most of the universities and research institutes have witnessed a long pause in basic science research ever. Much has been discussed about the long-term impact of COVID-19 on the economy, tourism, public health, small and large-scale businesses of several kinds. However, the long-term effects of the shut-down of these research labs and their impact on basic science research has not been much focused. Herein, we provide a perspective that portrays a common problem of all the basic science researchers throughout the globe and its long-term consequences.

Published
2021

39. Neuroprotective effect of phospholipase A2 from Malaysian Naja sumatrana venom against H2O2-induced cell damage and apoptosis.

Author

Haizum Abdullah, Nur Atiqah, Afifah Veronica Sainik, Nur Qisya, Esa, Ezalia, Muhamad Hendri, Nur Afrina, Ahmad Rusmili, Muhamad Rusdi, Hodgson, Wayne C., Shaikh, Mohd Farooq, and Othman, Iekhsan

Subjects
COBRAS, VENOM, OXIDATIVE stress, CELL survival, APOPTOSIS, NEUROPROTECTIVE agents
Abstract

Oxidative stress is one of the factors involved in the pathogenesis of several neurodegenerative diseases. It has been reported that a secretory phospholipase A2 known as A2-EPTX-NSm1a has lower cytotoxicity in neuronal cells compared to its crude Naja sumatrana venom. In this study, A2-EPTX-NSm1a was tested for its neuroprotective activity on human neuroblastoma cells (SH-SY5Y) differentiated into cholinergic neurons against oxidative stress induced by hydrogen peroxide (H2O2). H2O2 treatment alone increased the caspase-3 and caspase-8 activities, whereas pre-treatment with A2-EPTX-NSm1a reduced the activity of these apoptosisassociated proteins. Moreover, A2-EPTX-NSm1a protects the morphology and ultrastructure of differentiated SH-SY5Y cells in the presence of H2O2. Oxidative stress increased the number of small mitochondria. Further evaluation showed the size of mitochondria with a length below 0.25 µm in oxidative stress conditions is higher than the control group, suggesting mitochondria fragmentation. Pre-treatment with A2-EPTX-NSm1a attenuated the number of mitochondria in cells with H2O2 Furthermore, A2-EPTX-NSm1a altered the expression of several neuroprotein biomarkers of GDNF, IL-8, MCP-1, TIMP-1, and TNF-R1 in cells under oxidative stress induced by H2O2. These findings indicate that anti-apoptosis with mitochondria-related protection, antiinflammatory effect, and promote expression of important markers for cell survival may underlie the neuroprotective effect of A2-EPTX-NSm1a in cholinergic rich human cells under oxidative stress, a vital role in the neuronal disorder. [ABSTRACT FROM AUTHOR]

Published
2022
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40. Antimicrobial Resistance Patterns and Risk Factors Associated with Salmonella spp. Isolates from Poultry Farms in the East Coast of Peninsular Malaysia: A Cross-Sectional Study

Author

Osman, Abdinasir Yusuf, primary, Elmi, Sharifo Ali, additional, Simons, David, additional, Elton, Linzy, additional, Haider, Najmul, additional, Khan, Mohd Azam, additional, Othman, Iekhsan, additional, Zumla, Alimuddin, additional, McCoy, David, additional, and Kock, Richard, additional

Published
2021
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44. A Biochemical and Pharmacological Characterization of Phospholipase A2 and Metalloproteinase Fractions from Eastern Russell’s Viper (Daboia siamensis) Venom: Two Major Components Associated with Acute Kidney Injury

Author

Chaisakul, Janeyuth, primary, Khow, Orawan, additional, Wiwatwarayos, Kulachet, additional, Rusmili, Muhamad Rusdi Ahmad, additional, Prasert, Watcharamon, additional, Othman, Iekhsan, additional, Abidin, Syafiq Asnawi Zainal, additional, Charoenpitakchai, Mongkon, additional, Hodgson, Wayne C., additional, Chanhome, Lawan, additional, and Chaiyabutr, Narongsak, additional

Published
2021
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