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The constitutive proteasome and the immunoproteasome represent validated targets for pharmacological intervention in the context of various diseases, such as cancer, inflammation, and autoimmune diseases. The development of novel chemical scaffolds of non-peptidic nature, capable of inhibiting different catalytically active subunits of both isoforms, is a viable approach against these diseases. Such compounds are also useful as leads for the development of biochemical probes that enable the studies of the roles of both isoforms in various biological contexts. Here, we present a ligand-based computational design of (immuno)proteasome inhibitors, which resulted in the amino-substituted N-arylpiperidine-based compounds that can inhibit different subunits of the (immuno)proteasome in the low micromolar range. The compounds represent a useful starting point for further structure-activity relationship studies that will, hopefully, lead to non-peptidic compounds that could be used in pharmacological and biochemical studies of both proteasomes.

Synthetic fertilizers are the main nitrogen (N) input used in specialty crop systems established on sandy soils of Florida, although organic amendments and fertilizers can be used as a substitute. Organic N contained in these products must be mineralized before crop uptake, which is affected by amendment properties, soil properties, and temperature. A better method for predicting N release can help maximize the nutrient cycling benefits of organic amendments and fertilizers while avoiding negative environmental impacts. The main objective of this study was to measure N release and CO2 emissions from two poultry manure-based amendments (PMA) and two processed organic fertilizers (OF) made from livestock byproducts (e.g., feather meal). We conducted an 8-week incubation using two sandy Florida soils belonging to two soil orders (Entisol and Spodosol) and with a greater than two-fold difference in soil organic C. We incubated these soils at 10 °C, 17 °C, 24 °C, and 30 °C, measured plant-available N at 0, 1, 4, and 8 weeks, and measured CO2 emissions weekly. In both soils, OF released more inorganic N and at a faster rate compared with PMA, but CO2 emissions were greater from PMA than OF. Nitrogen mineralization and CO2 emissions increased with temperature, but temperature effects were less important than expected. These results on the mineralization of PMA and OF in sandy soils are key to optimize their use and management in Florida and other areas dominated by sandy soils.

Synthetic fertilizers are the main nitrogen (N) input used in specialty crop systems established on sandy soils of Florida, although organic amendments and fertilizers can be used as a substitute. Organic N contained in these products must be mineralized before crop uptake, which is affected by amendment properties, soil properties, and temperature. A better method for predicting N release can help maximize the nutrient cycling benefits of organic amendments and fertilizers while avoiding negative environmental impacts. The main objective of this study was to measure N release and CO2 emissions from two poultry manure-based amendments (PMA) and two processed organic fertilizers (OF) made from livestock byproducts (e.g., feather meal). We conducted an 8-week incubation using two sandy Florida soils belonging to two soil orders (Entisol and Spodosol) and with a greater than two-fold difference in soil organic C. We incubated these soils at 10 °C, 17 °C, 24 °C, and 30 °C, measured plant-available N at 0, 1, 4, and 8 weeks, and measured CO2 emissions weekly. In both soils, OF released more inorganic N and at a faster rate compared with PMA, but CO2 emissions were greater from PMA than OF. Nitrogen mineralization and CO2 emissions increased with temperature, but temperature effects were less important than expected. These results on the mineralization of PMA and OF in sandy soils are key to optimize their use and management in Florida and other areas dominated by sandy soils. [ABSTRACT FROM AUTHOR]

Societal Impact Statement The United Nations' Sustainable Development Goal (SDG) 17 calls for “strong global partnerships and cooperation” to support the other SDGs. The collections‐based science community offers many examples of conservation of plant and fungal biodiversity, sharing, repatriation and aggregation of data, access to new technologies, supply of plant and fungal material, strengthening capacity of practitioners, and benefit sharing with the providers of biodiversity and genetic resources. Collaboration framed by workable multilateral treaties will increase our understanding of plant and fungal diversity, help halt biodiversity loss, and accelerate our sustainable use of plants and fungi and the exploration of their useful traits. Summary Collections‐based institutes are at the forefront of generating knowledge and understanding of plant and fungal biodiversity. Through the analysis of occurrence data, the use of modern technologies to better understand the evolutionary relationships between species and documentation of their useful properties, the work of collections‐based institutes provides good models for conservation; addressing species loss and improving sustainable use of plants and fungi. Nevertheless, the pressure on the planet's plant and fungal diversity is relentless. We argue that a massive increase in the accessibility of preserved and living collections of plants and fungi is required. An increased scale of responsible exploration to both conserve and unlock the useful properties of plants and fungi is needed to deliver solutions to the many global challenges facing humanity and the planet. This article explores the role of collaborations between collections‐based institutes and their partners in preventing biodiversity loss and delivering sustainable development. Drawing on examples from herbaria, agricultural and wild species genebanks, mycological collections, an international NGO, and the botanic garden community, we demonstrate how collaboration improves efficiency and impact. Collaborations can be peer to peer, institutional, governmental, national, or international, they may involve work with local communities and are frequently a combination of these. We suggest the five key benefits to collaboration and show that with trust, understanding, and mutual respect, powerful and sustainable partnerships develop. Such trust and respect are hard won, but once established, sustain a high level of commitment, enable development of shared long‐term visions of success, and attract diverse funding streams.

The Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, and accessibility of germplasm collections of crop wild relatives (CWR). Between 2013 and 2018, partners in 25 countries, heirs to the globetrotting legacy of Nikolai Vavilov, undertook seed collecting expeditions targeting CWR of 28 crops of global significance for agriculture. Here, we describe the implementation of the 25 national collecting programs and present the key results. A total of 4587 unique seed samples from at least 355 CWR taxa were collected, conserved ex situ, safety duplicated in national and international genebanks, and made available through the Multilateral System (MLS) of the International Treaty on Plant Genetic Resources for Food and Agriculture (Plant Treaty). Collections of CWR were made for all 28 targeted crops. Potato and eggplant were the most collected genepools, although the greatest number of primary genepool collections were made for rice. Overall, alfalfa, Bambara groundnut, grass pea and wheat were the genepools for which targets were best achieved. Several of the newly collected samples have already been used in pre-breeding programs to adapt crops to future challenges.

Adhesion receptors, such as CD44, have been shown to activate receptor interacting protein kinase-3 (RIPK3)—mixed lineage kinase-like (MLKL) signaling, leading to a non-apoptotic cell death in human granulocyte/macrophage colony-stimulating factor (GM-CSF) – primed neutrophils. The signaling events of this necroptotic pathway, however, remain to be investigated. In the present study, we report the design, synthesis, and characterization of a series of novel serine protease inhibitors. Two of these inhibitors, compounds 1 and 3, were able to block CD44-triggered necroptosis in GM-CSF-primed neutrophils. Both inhibitors prevented the activation of MLKL, p38 mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3’—kinase (PI3K), hence blocking the increased levels of reactive oxygen species (ROS) required for cell death. Although compounds one and three partially inhibited isolated human neutrophil elastase (HNE) activity, we obtained no pharmacological evidence that HNE is involved in the initiation of this death pathway within a cellular context. Interestingly, neither serine protease inhibitor had any effect on FAS receptor-mediated apoptosis. Taken together, these results suggest that a serine protease is involved in non-apoptotic CD44-triggered RIPK3-MLKL-dependent neutrophil cell death, but not FAS receptor-mediated caspase-dependent apoptosis. Thus, a pharmacological block on serine proteases might be beneficial for preventing exacerbation of disease in neutrophilic inflammatory responses.

Effective irrigation scheduling enables the irrigator to apply the right amount of water at the right time to meet the crop water demand. This 19-page guide presents information on average daily and weekly crop water use and crop growth stages for twelve north Florida crops that can be used to help schedule irrigation. This will allow a grower to develop a realistic irrigation schedule that minimizes plant water stress, saves water, and reduces nutrient leaching potential. Written by Vivek Sharma, Charles Barrett, De Broughton, and Thomas Obreza, and published by the UF/IFAS Department of Soil and Water Sciences, revised December 2020.

This is an update by Andrea Albertin. Original publication: Shukla, Shuchi, and Thomas Obreza. 1. “Preparing and Storing an Emergency Safe Drinking Water Supply”. EDIS 2004 (18). https://journals.flvc.org/edis/article/view/114124.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

The information provided in the 2008 2nd edition is still sound for healthy citrus trees under Florida production conditions. Much of the information provided in this document on nutrients, application methods, leaf and soil sampling and irrigation scheduling are also effective for huanglongbing (HLB) affected citrus trees. However, research conducted since HLB was detected in Florida in 2005 has established changes in many production practices, including nutrient rates, irrigation scheduling, soil pH management, and use of Citrus Under Protective Screen (CUPS). Changes to the 2nd edition of SL253 will appear in boxes similar to this one at the beginnings of chapters 2, 6, 8, 9, and 11.

Legionella pneumophila is the causative agent of severe Legionnaires’ disease (LD). Although an increasing number of LD cases have been observed, published data from Slovenia are very limited and data on molecular epidemiology are even scarcer. The present retrospective study (2006–2020) reports the results of the microbiological diagnosis of LD, as well as the epidemiology and characterization of the Legionella clinical isolates. We tested urine samples from 15,540 patients with pneumonia symptoms for L. pneumophila infection by urine antigen test, of which 717 (4.6%) tested positive. Isolation of L. pneumophila was successfully performed from 88 clinical specimens, with 82 (93.2%) being identified as L. pneumophila sg 1 and six (6.8%) as L. pneumophila sg 2–14. Sequence-based typing (SBT) identified 33 different sequence types (STs), the most frequent being ST1 and ST23. Sequence type 1 mainly comprised isolates belonging to the Philadelphia subgroup, and ST23 mostly to Allentown/France. The standard SBT scheme, as well as Dresden phenotyping for L. pneumophila, presented a high diversity among isolates.

Using rescaffolding approach, we designed piperidine compounds decorated with an electrophilic oxathiazol-2-one moiety that is known to confer selectivity towards threonine proteases. Our efforts to prepare products according to the published procedures were not successful. Furthermore we identified major side products containing nitrile functional group, resulting from carboxamide dehydration. We systematically optimized reaction conditions towards our desired products to identify heating of carboxamides with chlorocarbonylsulfenyl chloride and sodium carbonate as base in dioxane at 100 °C. Our efforts culminated in the preparation of a small series of piperidin-3-yl-oxathiazol-2-ones that are suitable for further biological evaluation.

This document is about a survey conducted to quantify the implementation of pesticide Best Management Practices (BMPs) in the Gulf Coast Production Area (GCPA) citrus industry. The survey results revealed that most pesticide BMPs are widely adopted, with 13 out of 19 BMPs practiced on at least 80% of the surveyed area. This high adoption rate underscores the growers' awareness of the environmental and health impacts of pesticide use and their commitment to mitigating these effects. Key BMPs, such as targeted pesticide spraying, measures to reduce spray drift, regular equipment inspections, and proper storage practices, are extensively implemented. An informative BMP manual has been developed, and ongoing education and cost-share programs are recommended to support continuous improvement and adaptation to new regulations. Future surveys are suggested to monitor progress and effectiveness of these initiatives. First published Aug. 2010.

Soils in Florida may contain excess soil phosphorus (P) resulting from fertilizer, manure or biosolids applications. Excess P in soil does not damage soil fertility but can be transported from agricultural and urban areas either dissolved in water that drains away or as particulate matter (attached to soil particles) that travels with eroding soil. Increased P in water bodies is recognized as one of the major factors responsible for eutrophication-related decrease in water quality. Most soils have the capacity to retain excess P. However, soils with low capacity to retain excess P are abundant in Florida. The use of drinking-water treatment residuals (WTR) to control excess phosphorus (P) in soils with limited P adsorption capacity has been explored as a potential low-cost method by which to reduce phosphorus losses to surface waters and a discussion of these explorations is presented here. The target audience for this publication includes state agencies, like the Florida Department of Environmental Protection (FLDEP), and water management districts trying to control P pollution, and those interested in nutrient management for agricultural and environmental purposes. This 6-page fact sheet is a minor revision written by Jonathan D. Judy, Maria L. Silveira, Sampson Agyin-Birikorang, and George A. O’Connor, and published by the UF/IFAS Department of Soil and Water Sciences, June 2019. SL 300/SS513: Drinking-Water Treatment Residuals to Control Phosphorus in Soils (ufl.edu)

This 5-page fact sheet is part of the 2019–2020 Florida Citrus Production Guide. This chapter on irrigation management of citrus is largely taken from guidelines provided in SL253 Chapter 9 on trees prior to citrus greening: SL253/SS478: Nutrition of Florida Citrus Trees, 3rd Edition. A section has been added to cover recent findings on water use of trees affected by citrus greening and the impact this would have on the irrigation management considerations. Written by D. M. Kadyampakeni, K. T. Morgan, M. Zekri, R. S. Ferrarezi, A. W. Schumann, and T. A. Obreza, and published by the Agronomy Department, March 2019. CPG12/CG093: 2022–2023 Florida Citrus Production Guide: Irrigation Management of Citrus Trees (ufl.edu)

Fertilization is an important aspect of growing citrus commercially. Citrus tree growth, health, and fruit production and quality are closely affected by plant nutrition. This 3-page fact sheet is part of the 2019–2020 Florida Citrus Production Guide. Written by M. Zekri, A. W. Schumann, T. Vashisth, D. M. Kadyampakeni, K. T. Morgan, B. Boman, and T. Obreza, and published by the Agronomy Department, March 2019. CMG14/CG092: 2022–2023 Florida Citrus Production Guide: Fertilizer Application Methods (ufl.edu)

The following description of citrus fertilizer uptake, soil and leaf testing and nutrient recommendations were taken from SL 253* and reflects citrus nutrient management for healthy trees prior to citrus greening or HLB entering Florida. Additional information on nutrients obtained since the publication was released appear in this document as "the effect of HLB" on various aspects of citrus nutrient management and are noted as such with published papers cited. *Nutrition of Florida Citrus Trees, 2nd Edition By Thomas A. Obreza and Kelly T. Morgan (2008) SL253/SS478: Nutrition of Florida Citrus Trees, 3rd Edition. (ufl.edu) This 9-page fact sheet is part of the 2019–2020 Florida Citrus Production Guide. Written by K. T. Morgan, D. M. Kadyampakeni, M. Zekri, A. W. Shumann, T. Vashisth, and T. A. Obreza, and published by the Agronomy Department, March 2019. CMG13/CG091: 2022–2023 Florida Citrus Production Guide: Nutrition Management for Citrus Trees (ufl.edu)

This article presents the development of a reversed-phase ultra-high-performance liquid chromatographic method for determining process-related impurities in ropinirole hydrochloride drug substance applying the analytical quality by design approach. The current pharmacopeial method suffers from selectivity issues due to two coelutions of two pairs of impurities. The development of a new method began with preliminary experiments, based on which the Acquity UPLC BEH C8 was selected as the most appropriate column. The effects of six different critical method parameters (CMPs) were then investigated using a fractional factorial screening design. Column temperature, the ratio of methanol in mobile phase B, and gradient slope turned out to be highly significant CMPs in achieving critical resolutions, and they were further evaluated using a central composite face-centered response-surface design. Mathematical models were created by applying a multiple linear regression method. Based on the elution order of an unknown degradation impurity and impurity C, two design spaces were established, and for each design space an optimal combination of CMPs was determined. The method developed was validated for precision, accuracy, linearity, and sensitivity, and it was proven suitable for determining nine process-related impurities of ropinirole.

Tomato (Solanum lycopersicum L.) is a high-value crop that has potential to enhance its P-use efficiency. While phosphorus (P) is an essential nutrient, supplies are finite and much of the P supply in agricultural soils is not bioavailable after application due to reactions such as soil adsorption, immobilization, or precipitation. Low-P bioavailability results in reduced growth, so plants may mobilize soil-bound P by altering root morphology, exuding root-derived compounds, or forming symbiosis with microorganisms. This review discusses (i) the significance of P in plants and agroecosystems, (ii) within-plant response to changing P bioavailabilities, and (iii) strategies to enhance P-acquisition efficiency (PAE). Phosphorus forms fluctuate in the soil and potential approaches to increase the bioavailable pool of P may focus on processes such as desorption, mineralization, or dissolving precipitated P-compounds. To enhance these processes, roots may alter their spatial arrangement, exude protons to acidify the rhizosphere, exude carboxylates to solubilize bound-P, exude phosphatase to mineralize organic P, or enhance symbiosis with native microbes. High PAE allows for use of accumulated soil P as opposed to relying on fertilizer application to meet crop demand.

This article presents the development of a reversed-phase (RP) high-performance liquid chromatographic (HPLC) method for determination of process-related impurities in a celecoxib drug substance following Analytical Quality by Design (AQbD) principles. The method from European Pharmacopeia (EP) for celecoxib drug substance does not sufficiently separate celecoxib from its EP impurity B because the system suitability criterion is not achieved (resolution NLT 1.8). The same issue was observed with the proposed method from United States Pharmacopeia (USP) for celecoxib capsules, where EP impurity A elutes under the main peak. A new HPLC method was developed that eliminates the disadvantages of the two pharmacopeial methods and is capable of efficiently separating and determining all seven impurities listed in EP and the proposed USP monographs. The development of a new HPLC method started with method scouting, in which various C18 and phenyl stationary phases were tested. Improved selectivity was obtained only with a chiral stationary phase. An immobilized Chiralpak IA-3 column used in RP mode turned out to be the most appropriate for method optimization. The ratio of acetonitrile in the mobile phase, flow rate, and column temperature were recognized as critical method parameters (CMPs) and were further investigated using a central composite face response-surface design. A multiple linear regression (MLR) method was applied to fit the mathematical models on the experimental data to determine factor−response relationships. The models created show adequate fit and good prediction abilities. The Monte Carlo simulation method was used to establish the design space. The method developed was verified in terms of precision, sensitivity, accuracy, and linearity, and the results showed that the new method is suitable for determination of seven process-related impurities of celecoxib.

This 3-page publication targets agricultural and horticultural producers, homeowners, Extension agents, industry or governmental staff, land managers, other professionals, youth and interested citizens. It is a minor revision written by Mongi Zekri, Thomas A. Obreza, Robert Koo, and Fernando M. Alferez, and published by the Soil and Water Science Department, April 2018. SL 207/SS426: Irrigation, Nutrition, and Citrus Fruit Quality (ufl.edu)