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Your search keyword '"Nita-Lazar, Aleksandra"' showing total 138 results
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138 results on '"Nita-Lazar, Aleksandra"'

15. Less Severe Sepsis in Cecal Ligation and Puncture Models with and without Lipopolysaccharide in Mice with Conditional Ezh2-Deleted Macrophages (LysM-Cre System)

Author

Phuengmaung, Pornpimol, primary, Khiewkamrop, Phuriwat, additional, Makjaroen, Jiradej, additional, Issara-Amphorn, Jiraphorn, additional, Boonmee, Atsadang, additional, Benjaskulluecha, Salisa, additional, Ritprajak, Patcharee, additional, Nita-Lazar, Aleksandra, additional, Palaga, Tanapat, additional, Hirankarn, Nattiya, additional, and Leelahavanichkul, Asada, additional

Published
2023
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22. Less Severe Lipopolysaccharide-Induced Inflammation in Conditional mgmt -Deleted Mice with LysM-Cre System: The Loss of DNA Repair in Macrophages.

Author

Saisorn, Wilasinee, Phuengmaung, Pornpimol, Issara-Amphorn, Jiraphorn, Makjaroen, Jiradej, Visitchanakun, Peerapat, Sae-khow, Kritsanawan, Boonmee, Atsadang, Benjaskulluecha, Salisa, Nita-Lazar, Aleksandra, Palaga, Tanapat, and Leelahavanichkul, Asada

Subjects
O6-Methylguanine-DNA Methyltransferase, DNA repair, DNA ligases, MACROPHAGES, SMALL interfering RNA, GENE silencing
Abstract

Despite the known influence of DNA methylation from lipopolysaccharide (LPS) activation, data on the O6-methylguanine-DNA methyltransferase (MGMT, a DNA suicide repair enzyme) in macrophages is still lacking. The transcriptomic profiling of epigenetic enzymes from wild-type macrophages after single and double LPS stimulation, representing acute inflammation and LPS tolerance, respectively, was performed. Small interfering RNA (siRNA) silencing of mgmt in the macrophage cell line (RAW264.7) and mgmt null (mgmtflox/flox; LysM-Crecre/−) macrophages demonstrated lower secretion of TNF-α and IL-6 and lower expression of pro-inflammatory genes (iNOS and IL-1β) compared with the control. Macrophage injury after a single LPS dose and LPS tolerance was demonstrated by reduced cell viability and increased oxidative stress (dihydroethidium) compared with the activated macrophages from littermate control mice (mgmtflox/flox; LysM-Cre−/−). Additionally, a single LPS dose and LPS tolerance also caused mitochondrial toxicity, as indicated by reduced maximal respiratory capacity (extracellular flux analysis) in the macrophages of both mgmt null and control mice. However, LPS upregulated mgmt only in LPS-tolerant macrophages but not after the single LPS stimulation. In mice, the mgmt null group demonstrated lower serum TNF-α, IL-6, and IL-10 than control mice after either single or double LPS stimulation. Suppressed cytokine production resulting from an absence of mgmt in macrophages caused less severe LPS-induced inflammation but might worsen LPS tolerance. [ABSTRACT FROM AUTHOR]

Published
2023
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23. Microbiota and adipocyte mitochondrial damage in type 2 diabetes are linked by Mmp12+ macrophages

Author

Li, Zhipeng, primary, Gurung, Manoj, additional, Rodrigues, Richard R., additional, Padiadpu, Jyothi, additional, Newman, Nolan K., additional, Manes, Nathan P., additional, Pederson, Jacob W., additional, Greer, Renee L., additional, Vasquez-Perez, Stephany, additional, You, Hyekyoung, additional, Hioki, Kaito A., additional, Moulton, Zoe, additional, Fel, Anna, additional, De Nardo, Dominic, additional, Dzutsev, Amiran K., additional, Nita-Lazar, Aleksandra, additional, Trinchieri, Giorgio, additional, Shulzhenko, Natalia, additional, and Morgun, Andrey, additional

Published
2022
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24. Species-Specific Endotoxin Stimulus Determines Toll-Like Receptor 4- and Caspase 11-Mediated Pathway Activation Characteristics

Author

Ernst, Orna, primary, Khan, Mohd M., additional, Oyler, Benjamin L., additional, Yoon, Sung Hwan, additional, Sun, Jing, additional, Lin, Fang-Yu, additional, Manes, Nathan P., additional, MacKerell, Alexander D., additional, Fraser, Iain D. C., additional, Ernst, Robert K., additional, Goodlett, David R., additional, and Nita-Lazar, Aleksandra, additional

Published
2021
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32. The Role of TLRs in Anti-cancer Immunity and Tumor Rejection

Author

Urban-Wojciuk, Zuzanna, Khan, Mohd M., Oyler, Benjamin L., Fåhraeus, Robin, Marek-Trzonkowska, Natalia, Nita-Lazar, Aleksandra, Hupp, Ted R., Goodlett, David R., Urban-Wojciuk, Zuzanna, Khan, Mohd M., Oyler, Benjamin L., Fåhraeus, Robin, Marek-Trzonkowska, Natalia, Nita-Lazar, Aleksandra, Hupp, Ted R., and Goodlett, David R.

Abstract

In recent years, a lot of scientific interest has focused on cancer immunotherapy. Although chronic inflammation has been described as one of the hallmarks of cancer, acute inflammation can actually trigger the immune system to fight diseases, including cancer. Toll-like receptor (TLR) ligands have long been used as adjuvants for traditional vaccines and it seems they may also play a role enhancing efficiency of tumor immunotherapy. The aim of this perspective is to discuss the effects of TLR stimulation in cancer, expression of various TLRs in different types of tumors, and finally the role of TLRs in anti-cancer immunity and tumor rejection.

Published
2019
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34. Lipocalin-2 (Lcn-2) Attenuates Polymicrobial Sepsis with LPS Preconditioning (LPS Tolerance) in FcGRIIb Deficient Lupus Mice

Author

Ondee, Thunnicha, primary, Gillen, Joseph, additional, Visitchanakun, Peerapat, additional, Somparn, Poorichaya, additional, Issara-Amphorn, Jiraphorn, additional, Dang Phi, Cong, additional, Chancharoenthana, Wiwat, additional, Gurusamy, Devikala, additional, Nita-Lazar, Aleksandra, additional, and Leelahavanichkul, Asada, additional

Published
2019
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39. The Legionella pneumophilaeffector DenR hijacks the host NRas proto-oncoprotein to downregulate MAPK signaling

Author

Lehman, Stephanie S., Williamson, Chad D., Tucholski, Trisha, Ellis, Nicole A., Bouchard, Sabrina, Jarnik, Michal, Allen, Morgan, Nita-Lazar, Aleksandra, and Machner, Matthias P.

Abstract

Small GTPases of the Ras subfamily are best known for their role as proto-oncoproteins, while their function during microbial infection has remained elusive. Here, we show that Legionella pneumophilahijacks the small GTPase NRas to the Legionella-containing vacuole (LCV) surface. A CRISPR interference screen identifies a single L. pneumophilaeffector, DenR (Lpg1909), required for this process. Recruitment is specific for NRas, while its homologs KRas and HRas are excluded from LCVs. The C-terminal hypervariable tail of NRas is sufficient for recruitment, and interference with either NRas farnesylation or S-acylation sites abrogates recruitment. Intriguingly, we detect markers of active NRas signaling on the LCV, suggesting it acts as a signaling platform. Subsequent phosphoproteomics analyses show that DenR rewires the host NRas signaling landscape, including dampening of the canonical mitogen-activated protein kinase pathway. These results provide evidence for L. pneumophilatargeting NRas and suggest a link between NRas GTPase signaling and microbial infection.

Published
2024
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42. Internal modifications in the CENP-A nucleosome modulate centromeric dynamics

Author

Bui, Minh, Bui, Minh, Pitman, Mary, Nuccio, Arthur, Roque, Serene, Donlin-Asp, Paul Gregory, Nita-Lazar, Aleksandra, Papoian, Garegin A., Dalal, Yamini, Bui, Minh, Bui, Minh, Pitman, Mary, Nuccio, Arthur, Roque, Serene, Donlin-Asp, Paul Gregory, Nita-Lazar, Aleksandra, Papoian, Garegin A., and Dalal, Yamini

Abstract

Posttranslational modifications of core histones are correlated with changes in transcriptional status, chromatin fiber folding, and nucleosome dynamics. However, within the centromere-specific histone H3 variant CENP-A, few modifications have been reported, and their functions remain largely unexplored. In this multidisciplinary report, we utilize in silico computational and in vivo approaches to dissect lysine 124 of human CENP-A, which was previously reported to be acetylated in advance of replication. Computational modeling demonstrates that acetylation of K124 causes tightening of the histone core and hinders accessibility to its C-terminus, which in turn diminishes CENP-C binding. Additionally, CENP-A K124ac/H4 K79ac containing nucleosomes are prone to DNA sliding. In vivo experiments using a CENP-A acetyl or unacetylatable mimic (K124Q and K124A, respectively) reveal alterations in CENP-C levels and a modest increase in mitotic errors. Furthermore, mutation of K124 results in alterations in centromeric replication timing. Purification of native CENP-A proteins followed by mass spectrometry analysis reveals that while CENP-A K124 is acetylated at G1/S, it switches to monomethylation during early S and mid-S phases. Finally, we provide evidence implicating the histone acetyltransferase (HAT) p300 in this cycle. Taken together, our data suggest that cyclical modifications within the CENP-A nucleosome contribute to the binding of key kinetochore proteins, the integrity of mitosis, and centromeric replication. These data support the paradigm that modifications in histone variants can influence key biological processes.

Published
2017

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