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In the last few years, there has been a growing interest in exploring the association between risk factors such as overweight, obesity and physical activity, and incidence of various cancers. Meta-analysis was performed to investigate the risk ratio of follicular lymphoma incidence and mortality in overweight and obese individuals, and in individuals with a different physical activity levels using the random-effects model. A literature search through September 2016 was performed. Case-control studies accounted for over 2.100 cases and 12.700 controls, whereas cohort studies accounted for over 2.600 cases in cohort of about 3.000.000 individuals. In overweight individuals (body mass index between 25 and 29.99 kg/m2) risk ratio for the development of follicular lymphoma was 1.03 (0.95-1.11; 95% CI; p = 0.51) and in obese (body mass index ≥ 30 kg/m2) it was 1.15 (1.01-1.31; 95% CI; p = 0.04) when compared to individuals with normal body mass index (< 25 kg/m2). The risk ratio of specific follicular lymphoma mortality in overweight was 0.59 (0.38-0.91; 95% CI; p = 0.02), while in obese patients it was 1.08 (0.68-1.71; 95% CI; p = 0.75). In patients with the highest physical activity levels, the risk ratio for follicular lymphoma occurrence was 0.95 (0.75-1.21; 95% CI; p = 0.68) when compared to patients that had the lowest physical activity levels. In summary, our meta-analysis has shown statistically significant direct association between obesity and follicular lymphoma incidence.

Archaeoacoustic and Electromagnetic research of ancient sites is becoming an established discipline. SB Research Group (SBRG) have been developing a new methodology over the last five years using a practical archaeoacoustic standard (SBSA), which helps to explain certain phenomena which are commonly found at “sacred sites”. Applying this expertise enabled better understanding of the specific EM and acoustic wave emissions present on Kanda Hill, Macedonia. Analyzing these emissions enables better understanding of what lies below the surface. The previous research paper (ARSA 2014) demonstrated evidence of various physical phenomena present at this ancient site and the artificial origin of this hill, which should now be reffered to as a tumulus. At the time of writing the presence of interior cavities in was suspected, possibly consisting of small number of chambers and passageways. This hypothesis is corroborated by the evidence presented in this paper.

Archaeoacoustic and Electromagnetic research of ancient sites is becoming an established discipline. SB Research Group (SBRG) have been developing a new methodology over the last five years using a practical archaeoacoustic standard (SBSA), which helps to explain certain phenomena which are commonly found at “sacred sites”. Applying this expertise enabled better understanding of the specific EM and acoustic wave emissions present on Kanda Hill, Macedonia. Analyzing these emissions enables better understanding of what lies below the surface. The previous research paper (ARSA 2014) demonstrated evidence of various physical phenomena present at this ancient site and the artificial origin of this hill, which should now be referred to as a tumulus. At the time of writing the presence of interior cavities in was suspected, possibly consisting of small number of chambers and passageways. This hypothesis is corroborated by the evidence presented in this paper.

Although surgical revascularization of the myocardium is routine and one of the most performed procedures in cardiac surgery, complications that occur during and after surgery, such as systemic inflammation and sepsis, as well as bleeding due to impaired platelet function, have a high share in such high mortality. The extracorporeal bloodstream system has a special role in these processes. Determination of levels of new biomarker for detection infectious complications - presepsin, with CRP and leukocyte formula, is just one of these diagnostic combinations of tests that allow assessment of the immune status of a cardiac patient and the need for further examination and / or therapy of patients who may potentially develop systemic inflammatory response syndrome (SIRS) or sepsis. On the other hand, the application of aggregometry gives precise data on the functional changes of platelets, which enables adequate care of complications in terms of increased postoperative bleeding or thrombosis. The study included 100 patients of both sexes, 22 women (21.6%) and 78 men (76.5%), mean age 64.63 ± 7.48 years. Patients were divided into two groups in relation to the use of extracorporeal blood circulation device. Postoperatively, there was a decrease in the number of erythrocytes (p 1800ml) was present in 15 patients (15 in the group where the extracorporeal circulatory system was applied, 17.64% of patients (9/51) met the clinical criteria for SIRS with elevated presepsin values of 600 pg / ml and more, while in the second group of patients 2 of 49 (4.08%) met the clinical and laboratory criteria for SIRS.The ECC system significantly affects the development of SIRS and inhibition of platelet function in patients where it was applied during surgery.

A possibility to develop sever systemic complications of acute pancreatitis(AP) demands timely assessment of the severity of the disease in order to make an optimal therapeutic approach and a correct prognosis of the disease. The aim of the study was to establish the role of certain individual inflammatory biomarkers and coagulation parameters in the prediction of AP severity, and also to propose a mathematical formula which allows there combined use for the same purpose. Methods. The prospective study included 92 patients with AP. The patients were divided into groups: mild, moderate and severe AP. Results. Based on the results of Mann-Whitney,s test, it can be concluded that a statistically significant difference between groups A(mild) and B(moderate and severe) for CRP (p

In classical Hodgkin’s lymphoma (cHL) the existing prognostic scoring systems do not include markers that adequately reflect the interaction of malignant Hodgkin and Reed-Sternberg (HRS) cells and tumor environment. The research objective is to determine the relationship between serum Galectin-1 (Gal-1) and soluble CD163 (sCD163) and the clinical status of patients with cHL, with special emphasis on the presence of relapse, progression, or resistance to the therapy applied. The research included 79 patients. Of this number, 63 were patients with cHL, and the control group consisted of 16 healthy volunteers. The study group of 63 patients with cHL included a subgroup of newly diagnosed patients without therapy, newly diagnosed patients with therapy, patients with relapse and progression of the disease, and primary refractory patients during 2014 and 2015. Analysis of the levels of sCD163 and sGal-1 within a group of patients suffering from cHL has shown that the values of both molecules were higher in relapsed patients and the subgroup with progressive disease comparing the subgroup of newly diagnosed patients without therapy or patients with therapy onset. Determination of Gal-1 and sCD163 levels is simple and reliable analysis that can contribute to the identification of high-risk patients with cHL, and deserves inclusion in current prognostic scoring systems.

Patients with diffuse large B cell non Hodgkin lymphoma (DLBCL) have a very heterogeneous clinical course and prognosis. There is a constant need to find new prognostic factors to assess risk in patients. Immunohistochemical biomarkers correlated with clinical and biochemical parameters, as well as prognostic scores may be predictors of risk patients. The main aim of this study was to analyze the survival of patients with diffuse large B- cell non Hodgkin lymphoma according to clinical prognostic factors and expression of proteins involved in the activation of immune response, cell cycle regulation and programmed cell death (Ki-67, Bcl-2, Bcl-6 , CD10, CD5, CD138, MUM1 and the presence of mutations L265P MyD88), by classifying patients according to current prognostic scoring systems comparing the clinical and immunological factors prognosis in patients with DLBCL, make a proposal for a prognostic scoring system that would unite the two groups of parameters. All patients was determined by clinical stage of the disease. During the study were collected and processed by clinical and laboratory parameters, that are known to have a prognostic value and that it was possible to collect for all patients included in the study. It is about gender, age, Ann Arbor clinical stage of disease, extranodal number of cities affected processes, performance status, presence of "B" symptoms, hemoglobin, platelet count, erythrocyte sedimentation rate, C-reactive protein levels, beta 2 microglobulin, serum albumin and serum LDH. Histopathological material consisted of 68 paraffin blocks. From immunohistochemical parameters were analyzed: CD5, CD10, Ki- 67, Bcl-2, Bcl-6, MUM1 by EnVision procedure. Molecular-genetic study of gene polymorphism MyD88 L265P was used genomic DNA isolated from fixed tissue in paraffin patients with DLBCL. Polymerization chain reaction were amplified by DNA segments within which the SNP is tested gene MyD88 L265P. Statistical analyzes included the use of nonparametric methods and parametric tests. Period of OS (overall survival), was studied using Kaplan-Meier analysis. A statistically significant difference in overall survival (OS) exists in relation to the age (the benefit under the age of 60 years), compared with gender (in favor of women) and the albumin concentration in serum (lower serum albumin levels in patients with DLBCL are associated with shorter OS, as they showed the greatest predictive value). In the prediction of the risk significance showed recategorized performance status (category : ≤2; > 2), recategorized clinical stage of the disease (limited and advanced DLBCL) and R- IPI. The analysis of restriction fragment length is determined homozygous, normal wild type genotype polymorphisms tested. All other clinical, biochemical and immunohistochemical parameters showed no predictive value in patients with DLBCL. in relation to the applied therapeutic protocol there is a strong statistical significance in overall survival in favor of the patients treated at R- CHOP protocol. We standardized PCR-RFLP method for Myd 88 L265P DNA samples isolated from paraffin molds. According to our knowledge there are no data available on the study Myd 88 L265P polymorphism in a population of patients with DLBCL in Serbia. In the population of patients with areas of southeast Serbia pathological signaling pathway which achieves mutation Myd 88 L265, is not responsible for the development DLBCL.Male gender, age over 60, low levels of serum albumin were negative predictor factors in patients with DLBCL. R-IPI score is in the era imunochemotherapy, useful prognostic score in relation to the IPI.

Interferons (IFN) are first described in the late fifties of the twentieth century as special proteins in the family of cytokines and induced by the host, after invasion virus. If you are transferred to the fresh tissue was observed antiviral activity, thanks to inhibit replication of the virus. In this appearance immunology known as "interference". Under normal conditions in the human body concentration of IFN-a was below detectable levels or because cells synthesize IFN or contain as its constituent element. IFN was divided in three classes: IFN-, IFN- and IFN-. Most mammalian cells formed in unequal quantities of IFN- and IFN- after stimulation with viruses or other agents, or inducers of the synthesis of IFN. In many countries, is still detained biotechnological process for the production of human IFN (hlFN) for the clinical application of human leukocytes allocated from canned whole blood units, not older than 6 hours. After centrifugation the procedure centrifugation devotes a buffy coat (leukocyte suspension) as a "side" -produkat. Further processing of "buffy coat" (BC) was purified from the red cell contamination, in order to obtain as pure substrate of human leukocytes, which is subjected to viral infection in order to stimulate the synthesis of hIFN-. Different viruses are used as inductors synthesis hIFN-, but in practice most often used "Sendai" virus, cultivated in horion-alantoic membrane of chicken embryos. Induction of synthesis hIFN- played on temperature of +37 ° C and lasts 18 hours. Refined suspension of human tal and practical. In fundamental respects got the time sequence data on the morphology, viability and induction capabilities of leucocytes, or their ability under the influence of the inductor synthesis hIFN- (or lyophilized virus hemagglutinin) higher yields of interferon, in the new proposed terms. In practical terms should consolidate that, when using a total amount of BC and from all transfusion centers in the Republic of Serbia as a raw material for the production of IFN-, in order to produce sufficient quantities IFN-, as biotherapeutic products, to cover not only needs Republic of Serbia, but to ensure the export of certain quantities of these products in countries with insufficient financial resources. Conclusions: 1. Learn yield and enhanced the antiviral activity of hIFN- in the compositions prepared from the suspension of leukocytes obtained from the pools "buffy coat"s which are added to dissolve dextran and 10% 6% hydroxy ethyl starch high molecular weight compared to the leukocyte suspension prepared in the conventional (current) method. 2. Better and more efficient biotechnological production process hIFN-, compared to the current procedure of obtaining hIFN- according to the modified "Cantell's" method. 3. The human leukocytes used for the production of hIFN- move more and have a greater ability to induction, while the same suspension of leukocytes is used for the production of hIFN- according to the modified "Cantell's" method has a stronger capacity of cell viability and induction ability of human leukocytes. 4. The lyophilized phytohemagglutinin - PHA-P, used as an inductor for the synthesis of hIFN- , although weaker than inductor "Sendai" virus, is a good inducer of the synthesis of which has been successfully induced human leukocytes in purified suspension.