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Teais one of the most significant plantation crops to be emphasized towards research on development for climate-resilient variants that suits across different climate crisisimpacted countries including India. Recent tea genome research advancements eased our thoughts to begin, and apply that addresses biotic, abiotic stresses and productivity. Though the effect of climate change with unpredictable weather conditions on tea crop’s resistance is unclear, DNA based and genomics-assisted breeding techniques might play prominent role in facing future challenges of crop improving set-ups. Transgene based technological advancements and molecular breeding strategies have simplified the progress of elite tea genotypes with robust adaptation to climate change and the genomics-assisted breeding strategies in specific, found to play a substantial part in the advance of climate resilient tea crops. In this review, we briefed the signs of progress in tea genome-based research and their further perspectives needed to address the current challenges we face due to the climate crisis that resolve to breed for the water-stress-tolerant tea plant.

Supplemental material, sj-docx-1-eeg-10.1177_15500594221138292 for Is There a Difference in EEG Characteristics in Acute, Chronic, and Experimentally Induced Musculoskeletal Pain States? a Systematic Review by Jerin Mathew, Tyson Michael Perez, Divya Bharatkumar Adhia, Dirk De Ridder and Ramakrishnan Mani in Clinical EEG and Neuroscience

Nanomaterial synthesis using natural biological systems, especially plant-based green synthesis has evinced great excitement and interest. In this study, iron oxide nanomaterials were synthesized from three different varieties of date palms obtained from Srivilliputhur, Tamil Nadu, India. The extract of date palms reduced ferric chloride in solution and facilitated the formation of iron oxide nanomaterials. Phytochemical studies of the extracts obtained from all three varieties of date palms indicated the presence of phenols. The synthesized nanomaterials were characterized by UV-VIS spectroscopy, SEM, EDAX, XRD and FTIR methods. The ability of the synthesized nanomaterials to inhibit human microbial pathogens proliferation was tested in Staphylococcus sp. and Bacillus sp. by turbidity method and recorded the maximum antimicrobial property against Bacillus sp. in the iron nanomaterial synthesized from Sukhri date palm. The cytotoxic potential of the iron oxide nanomaterials was assessed against two human cancer cell lines (A-549 and MDA-MB-231) by MTT assay. The substantial cytotoxicity response was detected from the iron oxide nanomaterials made from Sukhri date palm.

Objective: The objective of this study is to alter the expression of p-glycoprotein (p-gp) pump proteins in HepG2 cells after treating with urea and β-mercaptoethanol (BME) (lead compounds). The most common cause for resistance to a broad range of anticancer drugs is influenced by overexpression of p-gp pumps that detect and eject anticancer drugs from the cancer cell. Altering the expression of these proteins will reduce the efflux action and enhance the drug retention eventually killing the cancer cell. Materials and Methods: 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyl tetrazolium bromide (MTT) assay was carried out to measure the cell viability (HepG2 cells) post-treatment with the lead compounds followed by flow cytometric analysis for protein expression studies. Results: MTT assay confirms that the viability of HepG2 cells reduces as the concentrations of the lead compounds are increased. Flow cytometric analysis confirms reduced p-gp expression in HepG2 cells post-treatment with urea and BME. Compare to BME, urea turns out to be a potential compound in altering the expression of p-gp. Conclusion: The present cell line study confirms that urea and BME are potential compounds which are able to reduce the p-gp expression inHepG2 cells.