79 results on '"Maggio, B"'
Search Results
2. Hexagonal phase with ordered acyl chains formed by a short chain asymmetric ceramide
- Author
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Dupuy, F.G., Fernández Bordín, S.P., Maggio, B., and Oliveira, R.G.
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- 2017
- Full Text
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3. Sound-Induced Flash Illusions Support Cortex Hyperexcitability in Fibromyalgia
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Di Stefano, V, Iacono, S, Gagliardo, A, Maggio, B, Guggino, G, Gangitano, M, Monastero, R, Maggio, V, Bolognini, N, Brighina, F, Di Stefano V., Iacono S., Gagliardo A., Maggio B., Guggino G., Gangitano M., Monastero R., Maggio V. R., Bolognini N., Brighina F., Di Stefano, V, Iacono, S, Gagliardo, A, Maggio, B, Guggino, G, Gangitano, M, Monastero, R, Maggio, V, Bolognini, N, Brighina, F, Di Stefano V., Iacono S., Gagliardo A., Maggio B., Guggino G., Gangitano M., Monastero R., Maggio V. R., Bolognini N., and Brighina F.
- Abstract
Objectives. Fibromyalgia (FM) is characterized by spontaneous chronic widespread pain in combination with hyperalgesia to pressure stimuli. Sound-induced flash illusions (SIFIs) reflect cross-modal interactions between senses allowing to assess a visual cortical hoerexcitability (VCH) by evaluating the fission and fusion illusions disruption. The aims of the present study were to explore whether SIFIs are perceived differently in patients with fibromyalgia as compared to healthy controls (HCs) and how migraine affects fission and fusion illusions in fibromyalgia. Methods. A single flash (F) accompanied by 0 to 4 beeps (B) was presented to induce the fission illusion while multiple flash (i.e., 2 to 4) accompanied by 0 or 1 beep was presented to induce fusion illusion. The mean number of perceived flashes in fission and fusion illusion trials was compared between the groups (i.e., FM, FM with migraine, and HCs) using repeated-measures analysis of variance. Medication history was recorded along with the administration of Fibromyalgia Impact Questionnaire and Hospital Anxiety and Depression scales. Results. Twenty-four patients with FM (mean age 51, 2 ± 10, 6 years; 22 females), seventeen patients with FM and migraine without aura (mean age 47.8 ± 11.4 years; 16 females; 13 chronic, 4 episodic migraine), and forty-one age- and sex-matched HCs (mean age 47.3 ± 6.9 years; 34 females) participated in the study. Fission and fusion illusory effects were detected in all the participants. However, in FM patients, the fission illusion was reduced and almost abolished as compared to HCs (1F1B, p=0.02; 1F2B, p<0.0001; 1F3B, p<0.0001; 1F4B, p=0.0001), while there were no differences between groups in fusion trials. Migraine did not affect the fission and the fusion illusions. Conclusion. Results from this study confirm that patients with FM have a VCH suggesting that the pathological changes in cortical excitability might have important roles in the pathophysiology of FM. S
- Published
- 2022
4. Il Fast-Track delle fratture di femore prossimale over 65: analisi dell’epicrisi e sviluppo di modelli organizzativi efficaci
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Calori, G. M., Colombo, M., Campi, A., Di Maggio, B., Caiaffa, V., Momoli, A., Caroli, F., Longo, G., Donelli, F., Randelli, F., De Simoni, P., Pellegrino, A., Falez, F., and Sessa, G.
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- 2018
- Full Text
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5. Surface interactions, thermodynamics and topography of binary monolayers of Insulin with dipalmitoylphosphatidylcholine and 1-palmitoyl-2-oleoylphosphatidylcholine at the air/water interface
- Author
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Grasso, E.J., Oliveira, R.G., and Maggio, B.
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- 2016
- Full Text
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6. Preliminary report in treatment of proximal humeral fracture with closed reduction and DOS external fixation System: a multicentric study
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Scaravilli G, Mercurio J, Grazioli A, Gioia C, D’Arienzo A, Toro G, Galvano N, Monteleone G, Battaglini G, Di Santo F, Sepe S, Pagliuca S, Cesarano E, Mastrobuono G, Italiano M, D’Arienzo M, Maniscalco P, Ciatti C, Di Maggio B., Scaravilli, G, Mercurio, J, Grazioli, A, Gioia, C, D’Arienzo, A, Toro, G, Galvano, N, Monteleone, G, Battaglini, G, Di Santo, F, Sepe, S, Pagliuca, S, Cesarano, E, Mastrobuono, G, Italiano, M, D’Arienzo, M, Maniscalco, P, Ciatti, C, and Di Maggio, B.
- Abstract
Introduction: Proximal humerus fractures are the seventh most frequent fracture in adults, and the third in patients over 65 years old, 5.7% of whole diagnosed fractures. Most of these fractures can be treated conservatively and achieve good results. However, more and more frequently we are confronted with dislo-cated and multifragmentary fractures, and with elderly and high functional demanding patients. In patients with osteoporosis and poor general conditions external fixation can be performed as rapid and mininvasive procedure with good outcome and low complication rates. The authors investigated the use of external fixa-tion in the treatment of proximal humerus fractures. The objective is to demonstrate the effectiveness of this method as a valid alternative to other surgical techniques. Materials and Methods: A multicentre study was conducted at 7 hospitals in Italy from 2014 through 2018. We recruited all proximal humeral fractures (as classified with the Neer system) that are surgically treated with the same external fixator DOS, for a total of 110 patients, evaluated later with Oxford Shoulder Scale (OSS) and disability of the arm, shoulder and hand score (DASH) at 1, 2 and 6 months. Results:The patients have passed from a score of 75,37 in the first month to a score of 29,47in the sixth month at the DASH and from 47,02 to 27,71 at the OSS. The data further confirm the increased incidence of these fractures in women and in a mean age of about 65. Conclusions:Al-though it does not represent the golden standard in the treatment of fractures of the proximal humerus, in our experience the minimal osteosynthesis with external fixator turned out to be a very valid help especially for the simplicity and speed of the method, as well as for the exciting functional results. sometimes superior to other methods. The preliminary results from the different centers have confirmed this hypothesis. We hope this will be a good starting point for further in-depth studies.
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- 2022
7. Monitoring of the radio galaxy M 87 during a low-emission state from 2012 to 2015 with MAGIC
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Acciari, V A, Ansoldi, Antonelli, S, L A, Arbet , Engels, Arcaro, A, Baack, C, Babić, D, Banerjee, A, Bangale, B, Barres , de , Almeida, P, Barrio, U, J A, Becerra , González, Bednarek, J, Bellizzi, W, Bernardini, L, Berti, E, Besenrieder, A, Bhattacharyya, J, Bigongiari, W, Biland, C, Blanch, A, Bonnoli, O, Bošnjak, G, Busetto, Ž, Carosi, G, Ceribella, R, Chai, G, Chilingaryan, Y, Cikota, A, Colak, S, S M, Colin, Colombo, U, Contreras, E, J L, Cortina, Covino, J, D’Elia, S, Da , Vela, V, Dazzi, P, De , Angelis, F, Lotto, A, Delfino, B, Delgado, M, Depaoli, J, Di , Pierro, D, Venere, F, Do , Souto , Espiñeira, L, Dominis , Prester, E, Donini, D, Dorner, A, Doro, D, Elsaesser, M, Fallah , Ramazani, D, Fattorini, V, Fernández-Barral, A, Ferrara, A, Fidalgo, G, Foffano, D, Fonseca, L, M V, Font, Fruck, L, Fukami, C, García , López, S, R J, Garczarczyk, Gasparyan, M, Gaug, S, Giglietto, M, Giordano, N, Godinović, F, Green, N, Guberman, D, Hadasch, D, Hahn, D, Herrera, A, Hoang, J, Hrupec, J, Hütten, D, Inada, M, Inoue, T, Ishio, S, Iwamura, K, Jouvin, Y, Kerszberg, L, Kubo, D, Kushida, H, Lamastra, J, Lelas, A, D, Leone, F., Lindfors, E., Lombardi, Longo, S, López, F, López-Coto, M, López-Oramas, R, Loporchio, A, Machado , Oliveira , Fraga, S, Maggio, B, Majumdar, C, Makariev, P, Mallamaci, M, Maneva, M, Manganaro, G, Mannheim, M, Maraschi, K, Mariotti, L, Martínez, M, Masuda, M, Mazin, S, Mićanović, D, Miceli, S, Minev, D, Miranda, M, J M, Mirzoyan, Molina, R, Moralejo, E, Morcuende, A, Moreno, D, Moretti, V, Munar-Adrover, E, Neustroev, P, Nigro, V, Nilsson, C, Ninci, K, Nishijima, D, Noda, K, Nogués, K, Nöthe, L, Nozaki, M, Paiano, S, Palacio, S, Palatiello, J, Paneque, M, Paoletti, D, Paredes, R, Peñil, Peresano, P, Persic, M, Prada , Moroni, M, P G, Prandini, Puljak, E, Rhode, I, Ribó, W, Rico, M, Righi, J, Rugliancich, C, Saha, A, Sahakyan, L, Saito, N, Sakurai, T, Satalecka, S, Schmidt, K, Schweizer, K, Sitarek, T, Šnidarić, J, Sobczynska, I, Somero, D, Stamerra, A, Strom, A, Strzys, D, Suda, M, Surić, Y, Takahashi, T, Tavecchio, M, Temnikov, F, Terzić, P, Teshima, T, Torres-Albà, M, Tosti, N, Tsujimoto, L, Vagelli, S, van , Scherpenberg, V, Vanzo, J, Acosta, G, M Vazquez, Vigorito, C F, Vitale, Vovk, V, Will, I, Zarić, M, Asano, D, Collaborators: K, Hada, Harris, K, D E, Giroletti, Jermak, M, H E, Madrid, J P, Massaro, Richter, F, Spanier, S, Steele, F, I A, Walker, R, C, European Space Agency, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), National Aeronautics and Space Administration (US), Agencia Estatal de Investigación (España), European Commission, Acciari, V. A., Ansoldi, S., Antonelli, L. A., Arbet Engels, A., Arcaro, C., Baack, D., Babic, A., Banerjee, B., Bangale, P., Barres de Almeida, U., Barrio, J. A., Becerra Gonzalez, J., Bednarek, W., Bellizzi, L., Bernardini, E., Berti, A., Besenrieder, J., Bhattacharyya, W., Bigongiari, C., Biland, A., Blanch, O., Bonnoli, G., Bosnjak, Z., Busetto, G., Carosi, R., Ceribella, G., Chai, Y., Chilingaryan, A., Cikota, S., Colak, S. M., Colin, U., Colombo, E., Contreras, J. L., Cortina, J., Covino, S., D'Elia, V., da Vela, P., Dazzi, F., de Angelis, A., de Lotto, B., Delfino, M., Delgado, J., Depaoli, D., Di Pierro, F., Di Venere, L., Do Souto Espineira, E., Dominis Prester, D., Donini, A., Dorner, D., Doro, M., Elsaesser, D., Fallah Ramazani, V., Fattorini, A., Fernandez-Barral, A., Ferrara, G., Fidalgo, D., Foffano, L., Fonseca, M. V., Font, L., Fruck, C., Fukami, S., Garcia Lopez, R. J., Garczarczyk, M., Gasparyan, S., Gaug, M., Giglietto, N., Giordano, F., Godinovic, N., Green, D., Guberman, D., Hadasch, D., Hahn, A., Herrera, J., Hoang, J., Hrupec, D., Hutten, M., Inada, T., Inoue, S., Ishio, K., Iwamura, Y., Jouvin, L., Kerszberg, D., Kubo, H., Kushida, J., Lamastra, A., Lelas, D., Leone, F., Lindfors, E., Lombardi, S., Longo, F., Lopez, M., Lopez-Coto, R., Lopez-Oramas, A., Loporchio, S., Machado de Oliveira Fraga, B., Maggio, C., Majumdar, P., Makariev, M., Mallamaci, M., Maneva, G., Manganaro, M., Mannheim, K., Maraschi, L., Mariotti, M., Martinez, M., Masuda, S., Mazin, D., Micanovic, S., Miceli, D., Minev, M., Miranda, J. M., Mirzoyan, R., Molina, E., Moralejo, A., Morcuende, D., Moreno, V., Moretti, E., Munar-Adrover, P., Neustroev, V., Nigro, C., Nilsson, K., Ninci, D., Nishijima, K., Noda, K., Nogues, L., Nothe, M., Nozaki, S., Paiano, S., Palacio, J., Palatiello, M., Paneque, D., Paoletti, R., Paredes, J. M., Penil, P., Peresano, M., Persic, M., Prada Moroni, P. G., Prandini, E., Puljak, I., Rhode, W., Ribo, M., Rico, J., Righi, C., Rugliancich, A., Saha, L., Sahakyan, N., Saito, T., Sakurai, S., Satalecka, K., Schmidt, K., Schweizer, T., Sitarek, J., Snidaric, I., Sobczynska, D., Somero, A., Stamerra, A., Strom, D., Strzys, M., Suda, Y., Suric, T., Takahashi, M., Tavecchio, F., Temnikov, P., Terzic, T., Teshima, M., Torres-Alba, N., Tosti, L., Tsujimoto, S., Vagelli, V., van Scherpenberg, J., Vanzo, G., Vazquez Acosta, M., Vigorito, C. F., Vitale, V., Vovk, I., Will, M., Zaric, D., Asano, K., Hada, K., Harris, D. E., Giroletti, M., Jermak, H. E., Madrid, J. P., Massaro, F., Richter, S., Spanier, F., Steele, I. A., Walker, R. C., 30588766 - Arcaro, Cornelia, 25433822 - Richter, Stephan, and 25161814 - Spanier, Felix Alexander
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galaxies [Gamma rays] ,Photon ,Radio galaxy ,VHE [gamma ray] ,radiation mechanisms non-thermal ,galaxies active ,galaxies individual M 87 ,galaxies jets ,gamma rays galaxies ,magnetic field ,Astrophysics ,jet [Galaxies] ,01 natural sciences ,Power law ,law.invention ,galaxies: individual: M 87 ,law ,flux [gamma ray] ,radiation mechanisms: non-thermal ,galaxies: active ,galaxies: jets ,gamma rays: galaxies ,Astrophysics - High Energy Astrophysical Phenomena ,010303 astronomy & astrophysics ,QC ,QB ,High Energy Astrophysical Phenomena (astro-ph.HE) ,astro-ph.HE ,Physics ,galaxie [Gamma rays] ,photon ,Synchrotron ,Magnetic field ,Wavelength ,Galaxies: active ,Galaxies: individual: M 87 ,Galaxies: jets ,Gamma rays: galaxies ,Radiation mechanisms: non-thermal ,Spectral energy distribution ,Astrophysics and Astronomy ,active [Galaxies] ,Astrophysics::High Energy Astrophysical Phenomena ,radio wave [galaxy] ,FOS: Physical sciences ,Astrophysics::Cosmology and Extragalactic Astrophysics ,GLAST ,GeneralLiterature_MISCELLANEOUS ,Telescope ,emission [gamma ray] ,synchrotron ,0103 physical sciences ,Astrophysics::Galaxy Astrophysics ,non-thermal [Radiation mechanisms] ,hybrid ,010308 nuclear & particles physics ,ComputingMilieux_PERSONALCOMPUTING ,individual: M 87 [galaxies] ,Astronomy and Astrophysics ,MAGIC ,monitoring ,Space and Planetary Science ,ddc:520 ,spectral ,jets [Galaxies] ,individual: M 87 [Galaxies] ,non-thermal [adiation mechanisms] - Abstract
MAGIC Collaboration: et al., M 87 is one of the closest (z = 0.004 36) extragalactic sources emitting at very high energies (VHE, E > 100 GeV). The aim of this work is to locate the region of the VHE gamma-ray emission and to describe the observed broad-band spectral energy distribution (SED) during the low VHE gamma-ray state. The data from M 87 collected between 2012 and 2015 as part of a MAGIC monitoring programme are analysed and combined with multiwavelength data from Fermi-LAT, Chandra, HST, EVN, VLBA, and the Liverpool Telescope. The averaged VHE gamma-ray spectrum can be fitted from ∼100 GeV to ∼10 TeV with a simple power law with a photon index of (−2.41 ± 0.07), while the integral flux above 300 GeV is (1.44±0.13)×10−12cm−2s−1. During the campaign between 2012 and 2015, M 87 is generally found in a low-emission state at all observed wavelengths. The VHE gamma-ray flux from the present 2012–2015M 87 campaign is consistent with a constant flux with some hint of variability (∼3σ) on a daily time-scale in 2013. The low-state gamma-ray emission likely originates from the same region as the flare-state emission. Given the broad-band SED, both a leptonic synchrotron self-Compton and a hybrid photohadronic model reproduce the available data well, even if the latter is preferred. We note, however, that the energy stored in the magnetic field in the leptonic scenario is very low, suggesting a matter-dominated emission region., The financial support of the German BMBF and MPG, the Italian Istituto Nazionale di Fisica Nucleare (INFN) and Istituto Nazionale di Astrofisica (INAF), the Swiss National Fund (SNF), the ERDF under the Spanish Ministry of Economy and Competitiveness (MINECO) (FPA2015-69818-P, FPA2012-36668, FPA2015-68378-P, FPA2015-69210-C6-2-R, FPA2015-69210-C6-4-R, FPA2015-69210-C6-6-R, AYA2015-71042-P, AYA2016-76012-C3-1-P, ESP2015-71662-C2-2-P, FPA2017-90566-REDC), the Indian Department of Atomic Energy, the Japanese JSPS and MEXT, and the Bulgarian Ministry of Education and Science, National RI Roadmap Project DO1-153/28.08.2018, is gratefully acknowledged. This work was also supported by the Spanish Centro de Excelencia ‘Severo Ochoa’ SEV-2016-0588 and SEV2015-0548, and Unidad de Excelencia ‘Maria de Maeztu’ MDM2014-0369, by the Croatian Science Foundation (HrZZ) Project IP-2016-06-9782 and the University of Rijeka Project 13.12.1.3.02, by the DFG Collaborative Research Centers grants SFB823/C4 and SFB876/C3, by the Polish National Research Centre grant UMO2016/22/M/ST9/00382, and by the Brazilian MCTIC, CNPq, and FAPERJ. The Very Long Baseline Array is operated by the National Radio Astronomy Observatory, which is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc. The work of FM is supported by the ‘Departments of Excellence 2018–2022’ Grant awarded by the Italian Ministry of Education, University and Research (MIUR) (L. 232/2016). His research has made also use of resources provided by the Compagnia di San Paolo for the grant awarded on the BLENV project (S1618 L1 MASF 01) and by the Ministry of Education, Universities and Research for the grant MASF FFABR 17 01. This investigation is supported by the National Aeronautics and Space Administration (NASA) grants GO4-15096X, AR6-17012X, and GO6-17081X. This work made use of the Swinburne University of Technology software correlator, developed as part of the Australian Major National Research Facilities Programme and operated under licence. Based in part on observations made with the NASA/European Space Agency (ESA) Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS5-26555. These observations are associated with programmes GO 12293, 12671, 13061, and 13759.
- Published
- 2020
8. Second primary neoplasms in patients with lung and gastroenteropancreatic neuroendocrine neoplasms: Data from a retrospective multi-centric study
- Author
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Massironi, S., Campana, D., Pusceddu c, S., Albertelli, M., Faggiano e, A., Panzuto f, F., Smiroldo g, V., Andreasi h, V., Rossi i, R. E., Maggio b, I., Torchio c, M., Dotto, A., Modica j, R., Rinzivillo f, M., Carnaghi k, C., Partelli h, S., Fanetti l, I., Lamberti b, G., Corti c, F., Ferone, D., Colao j, A., Annibale f, B., M, Invernizzi a, P., Falconi h, M., and ItaNet (Italian Association for Neuroendocrine Tumours) Study Group
- Published
- 2020
9. Sintesi e valutazione biologica di nuovi nitroderivati analoghi della pirrolomicina D attivi su Pseudomonas aeruginosa
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Agnese, R., Cusimano, M., Plescia, F., Maggio, B., Daidone, G., Raffa, D., LA FRANCA, M., LI PETRI, G., Ornella, R., Roberta, L., Barone, G., Schillaci, D., and Raimondi, M.
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Pirrolomina D ,Pseudomonas aeruginosa ,MIC ,Settore CHIM/03 - Chimica Generale E Inorganica ,Settore BIO/19 - Microbiologia Generale ,Settore CHIM/08 - Chimica Farmaceutica - Published
- 2018
10. CIO: 360° in trauma care
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Maniscalco, P., primary, Benazzo, F., additional, Ruggieri, P., additional, and Di Maggio, B., additional
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- 2019
- Full Text
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11. Synthesized benzamido derivatives exert antiproliferative effects by DNA damage and ROS generation
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D’Anneo, A., Raffa, D., Plescia, F., Maggio, B., Raimondi, M., Daidone, G., Lauricella, M., D’Anneo, A, Raffa, D, Plescia, F, Maggio, B, Raimondi, MV, Daidone, G, and Lauricella, M.
- Subjects
anti-proliferative effect ,DNA damage ,benzamido derivatives ,ROS ,anti-proliferative effects - Abstract
In this study we explored the effect and the biological action of synthesized benzamido derivatives bearing the (1S,2S)-2-phenyl-cyclopropane-1-carboxamido, 1,1'-biphen-2-carboxamido and 1,1'-biphen-4-carboxamido moieties on K562, a human leukemia cell line. Among the synthesized compounds a particular antiproliferative action was observed with the benzamido derivative bearing the 2-1,1'-biphenyl moiety with the substitution at the 5 position of the benzamido moiety with iodine. This compound showed cytotoxic effects in K562 leukemic cells at nanomolar concentrations and was, therefore, chosen as compound to explore its mode of action. Our analyses provided evidence that this benzamido derivative induced a reduction in cell number and volume with the appearance of a shrunken cytoplasm at 24h, followed by a widespread cell fragmentation at 48h. As demonstrated by flow cytometry analyses, the effect was dose- and time-dependent, causing a G2/M cell cycle arrest in the first phase of treatment (24h), followed by an apoptotic death at 48h (IC50 0.5 μM ). The elucidation of the underlying mechanism also disclosed that DNA arrest in G2/M phase of cell cycle was consequent to DNA lesions, since an increase in phospho-ATM and yH2AX, two known markers of DNA repair response system, was observed. Prolonging the time of treatment, the effects, which were observed only in leukaemic cells but not in normal bronchial epithelial cells, were accompanied by ROS production, JNK phosphorylation and induction of a caspase-dependent apoptosis.
- Published
- 2016
12. A Multicentric Prospective Incidence Study of Guillain-Barré Syndrome in Italy. The ITANG Study
- Author
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Benedetti, M, Pugliatti, M, Dalessandro, R, BEGHI, ETTORE, Chiò, A, Logroscino, G, Filippini, G, Galeotti, F, Massari, M, Santuccio, C, Raschetti, R, Abruzzi, L, Agazzi, E, Agostoni, E, Ambrogio, L, Amidei, S, Arbasino, C, Argentiero, V, Arnaboldi, M, Baldini, D, Barki, R, Bassi, P, Basso, F, Belcastro, V, Bellotti, M, Bersano, E, Besana, R, Bettoni, L, Bezzi, G, Bianconi, C, Bondavalli, M, Bonometti, A, Borghi, AM, Borsato, C, Bortolotto, S, Bottacchi, EF, Bresolin, N, Bruno, S, Burlina, A, Cafasso, G, Callegarini, C, Calvo, A, Candeloro, E, Casano, A, Cattaneo, SI, Cavallo, R, Cheldi, A, Ciardo, G, Cirignotta, F, Clerici, AM, Clerici, R, Comi, G, Conti, R, Coppo, F, Covelli, V, Crespi, V, Currò Dossi, M, Curtò, NA, D'Adda, E, Dallocchio, C, D'Anna, S, De Massis, P, De Toni Franceschini, L, Di Vito, N, Didonè, G, Dileone, M, Donati, E, Dotta, M, Fazio, R, Federico, F, FERRARESE, CARLO, Ferrazzini, F, Ferrero, B, Filosto, M, Frasson, E, Fusina, S, Galbussera, A, Gastaldo, E, Geda, C, Ghiglione, P, Giometto, B, Gionco, M, Giorgetti, A, Giussani, G, Gobbin, F, Grampa, G, Granieri, E, Greco, G, Guidetti, D, Guidi, C, Guidotti, M, Gusmaroli, G, Imperiale, D, Internò, S, Jann, S, La Spina, I, Leo, A, Leone, M, Leoni, S, Leotta, D, Lerario, R, Liotta, G, Livrea, P, Luda di Cortemiglio, E, Maggio, B, Magni, E, Magnoni, A, Maistrelli, J, Manca, D, Mandrioli, J, Manera, U, Marcello, N, Marchi, P, Marchini, C, Marconi, S, Mattioli, M, Mauro, A, Mazzaglia, G, Medici, D, Meineri, P, Meola, G, Micaglio, G, Michelucci, R, Michieli, G, Micieli, G, Minardi, C, Moglia, C, Monaco, S, Montanari, E, Moretto, G, Munerati, V, Mura, G, Mussutto, V, Nascimbene, C, Neri, W, Nichelli, P, Nobile Orazio, E, Oddenino, E, Onorato, S, Padovani, A, Palermo, M, Papurello, DM, Passarella, B, Pavesi, G, Penza, MT, Perini, M, Perini, F, Perla, F, Perlotto, N, Perrone, P, Pignatta, P, Pisano, F, Poglio, F, Polo, A, Poloni, M, Porazzi, D, Pradotto, L, Previdi, P, Quatrale, R, Rasi, F, Ravasio, A, Ravetti, C, Repaci, M, Riccardi, T, Riguzzi, P, Rinaldi, R, Riva, M, Romeo, V, Romorini, A, Rosso, T, Rotondo, G, Sacquegna, T, Sanson, F, Santamato, V, Santoro, D, Sartori, V, Sasanelli, F, Savio, K, Serena, M, Silani, V, Silvestri, L, Simioni, V, Squintani, GM, Suardelli, M, Tartagla, L, Terenghi, F, Terlizzi, E, Terzano, M, Tesser, F, Testa, L, Ticca, A, Ticozzi, N, Tiriticco, M, Tola, MR, Tonietti, S, Trianni, G, Trojano, M, Trotta, F, Turatti, M, Ursino, E, Vanotti, A, Vercellino, M, Villani, A, Vitelli, E, Zambito Marsala, S, Zanette, G, Zarcone, D, Zimatore, G, Zoccolella, S., Benedetti, Md, Pugliatti, M, D'Alessandro, R, Beghi, E, Chiò, A, Logroscino, G, Filippini, G, Galeotti, F, Massari, M, Santuccio, C, Comi, Giancarlo, Raschetti, R, ITANG Study, Group, Giometto, B, Benedetti, M, Dalessandro, R, Abruzzi, L, Agazzi, E, Agostoni, E, Ambrogio, L, Amidei, S, Arbasino, C, Argentiero, V, Arnaboldi, M, Baldini, D, Barki, R, Bassi, P, Basso, F, Belcastro, V, Bellotti, M, Bersano, E, Besana, R, Bettoni, L, Bezzi, G, Bianconi, C, Bondavalli, M, Bonometti, A, Borghi, A, Borsato, C, Bortolotto, S, Bottacchi, E, Bresolin, N, Bruno, S, Burlina, A, Cafasso, G, Callegarini, C, Calvo, A, Candeloro, E, Casano, A, Cattaneo, S, Cavallo, R, Cheldi, A, Ciardo, G, Cirignotta, F, Clerici, A, Clerici, R, Comi, G, Conti, R, Coppo, F, Covelli, V, Crespi, V, Currò Dossi, M, Curtò, N, D'Adda, E, Dallocchio, C, D'Anna, S, De Massis, P, De Toni Franceschini, L, Di Vito, N, Didonè, G, Dileone, M, Donati, E, Dotta, M, Fazio, R, Federico, F, Ferrarese, C, Ferrazzini, F, Ferrero, B, Filosto, M, Frasson, E, Fusina, S, Galbussera, A, Gastaldo, E, Geda, C, Ghiglione, P, Gionco, M, Giorgetti, A, Giussani, G, Gobbin, F, Grampa, G, Granieri, E, Greco, G, Guidetti, D, Guidi, C, Guidotti, M, Gusmaroli, G, Imperiale, D, Internò, S, Jann, S, La Spina, I, Leo, A, Leone, M, Leoni, S, Leotta, D, Lerario, R, Liotta, G, Livrea, P, Luda di Cortemiglio, E, Maggio, B, Magni, E, Magnoni, A, Maistrelli, J, Manca, D, Mandrioli, J, Manera, U, Marcello, N, Marchi, P, Marchini, C, Marconi, S, Mattioli, M, Mauro, A, Mazzaglia, G, Medici, D, Meineri, P, Meola, G, Micaglio, G, Michelucci, R, Michieli, G, Micieli, G, Minardi, C, Moglia, C, Monaco, S, Montanari, E, Moretto, G, Munerati, V, Mura, G, Mussutto, V, Nascimbene, C, Neri, W, Nichelli, P, Nobile Orazio, E, Oddenino, E, Onorato, S, Padovani, A, Palermo, M, Papurello, D, Passarella, B, Pavesi, G, Penza, M, Perini, M, Perini, F, Perla, F, Perlotto, N, Perrone, P, Pignatta, P, Pisano, F, Poglio, F, Polo, A, Poloni, M, Porazzi, D, Pradotto, L, Previdi, P, Quatrale, R, Rasi, F, Ravasio, A, Ravetti, C, Repaci, M, Riccardi, T, Riguzzi, P, Rinaldi, R, Riva, M, Romeo, V, Romorini, A, Rosso, T, Rotondo, G, Sacquegna, T, Sanson, F, Santamato, V, Santoro, D, Sartori, V, Sasanelli, F, Savio, K, Serena, M, Silani, V, Silvestri, L, Simioni, V, Squintani, G, Suardelli, M, Tartagla, L, Terenghi, F, Terlizzi, E, Terzano, M, Tesser, F, Testa, L, Ticca, A, Ticozzi, N, Tiriticco, M, Tola, M, Tonietti, S, Trianni, G, Trojano, M, Trotta, F, Turatti, M, Ursino, E, Vanotti, A, Vercellino, M, Villani, A, Vitelli, E, Zambito Marsala, S, Zanette, G, Zarcone, D, Zimatore, G, and Zoccolella, S
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Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Epidemiology ,Population ,Guillain-Barre Syndrome ,Rate ratio ,NO ,Young Adult ,Axonal and demyelinating GBS ,Guillain-Barré syndrome ,Incidence ,Prospective study ,Trend ,Neurology (clinical) ,Aged ,Aged, 80 and over ,Female ,Humans ,Italy ,Middle Aged ,80 and over ,medicine ,Young adult ,education ,Prospective cohort study ,education.field_of_study ,Guillain-Barre syndrome ,business.industry ,Medicine (all) ,Incidence (epidemiology) ,medicine.disease ,Vaccination ,Settore MED/26 - NEUROLOGIA ,business ,Human - Abstract
Background: To assess Guillain-Barré syndrome (GBS) incidence we relied on the Italian Network for the study of GBS (ITANG) established in 2010 in 7 Italian regions to analyse the association between influenza vaccination and GBS. Methods: All individuals aged ≥18 years, presenting with clinical manifestations that suggested GBS according to the universally accepted Asbury's diagnostic criteria (1990) were prospectively notified to a centralised database by ITANG neurologists over the period October 1, 2010-September 30, 2011. Through a telephone survey, 9 trained interviewers followed up the cases to diagnosis and then for 1 year since hospital discharge. Validation of case reporting was performed with the support of administrative data in 5 regions. Results: We found 365 cases fulfilling the definition for GBS or one of its variants over 19,846,068 population ≥18 years of age, yielding an annual incidence rate of 1.84 per 100,000 (95% CI 1.65-2.03), 2.30 (95% CI 1.99-2.60) in men and 1.41 (95% CI 1.18-1.64) in women. A highly significant peak of incidence was observed in February 2011 as compared to reference month (September 2011, rate ratio 3.3:1, p < 0.01). Conclusions: In Italy, GBS incidence was among the highest reported in Europe and higher than previously observed in Italian studies.
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- 2015
13. A Multicentric Prospective Incidence Study of Guillain-Barre Syndrome in Italy. the ITANG Study
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Benedetti, M. D., Pugliatti, M., Dalessandro, R., Beghi, E., Chio, A., Logroscino, G., Filippini, G., Galeotti, F., Massari, M., Santuccio, C., Raschetti R., Abruzzi L, Agazzi, E, Agostoni, E, Ambrogio, L, Amidei, S, Arbasino, C, Argentiero, V, Arnaboldi, M, Baldini, D, Barki, R, Bassi, P, Basso, F, Belcastro, V, Bellotti, M, Bersano, E, Besana, R, Bettoni, L, Bezzi, G, Bianconi, C, Bondavalli, M, Bonometti, A, Borghi, Am, Borsato, C, Bortolotto, S, Bottacchi, Ef, Bresolin, N, Bruno, S, Burlina, A, Cafasso, G, Callegarini, C, Calvo, A, Candeloro, E, Casano, A, Cattaneo, Si, Cavallo, R, Cheldi, A, Ciardo, G, Cirignotta, F, Clerici, Am, Clerici, R, Comi, G, Conti, R, Coppo, F, Covelli, V, Crespi, V, Currò Dossi, M, Curtò, Na, D'Adda, E, Dallocchio, C, D'Anna, S, De Massis, P, De Toni Franceschini, L, Di Vito, N, Didonè, G, Dileone, M, Donati, E, Dotta, M, Fazio, R, Federico, F, Ferrarese, C, Ferrazzini, F, Ferrero, B, Filosto, M, Frasson, E, Fusina, S, Galbussera, A, Gastaldo, E, Geda, C, Ghiglione, P, Giometto, B, Gionco, M, Giorgetti, A, Giussani, G, Gobbin, F, Grampa, G, Granieri, E, Greco, G, Guidetti, D, Guidi, C, Guidotti, M, Gusmaroli, G, Imperiale, D, Internò, S, Jann, S, La Spina, I, Leo, A, Leone, M, Leoni, S, Leotta, D, Lerario, R, Liotta, G, Livrea, P, Luda di Cortemiglio, E, Maggio, B, Magni, Eugenio, Magnoni, A, Maistrelli, J, Manca, D, Mandrioli, J, Manera, U, Marcello, N, Marchi, P, Marchini, C, Marconi, S, Mattioli, M, Mauro, A, Mazzaglia, G, Medici, D, Meineri, P, Meola, G, Micaglio, G, Michelucci, R, Michieli, G, Micieli, G, Minardi, C, Moglia, C, Monaco, S, Montanari, E, Moretto, G, Munerati, V, Mura, G, Mussutto, V, Nascimbene, C, Neri, W, Nichelli, P, Nobile-Orazio, E, Oddenino, E, Onorato, S, Padovani, A, Palermo, M, Papurello, Dm, Passarella, B, Pavesi, G, Penza, Mt, Perini, M, Perini, F, Perla, F, Perlotto, N, Perrone, P, Pignatta, P, Pisano, F, Poglio, F, Polo, A, Poloni, M, Porazzi, D, Pradotto, L, Previdi, P, Quatrale, R, Rasi, F, Ravasio, A, Ravetti, C, Repaci, M, Riccardi, T, Riguzzi, P, Rinaldi, R, Riva, M, Romeo, V, Romorini, A, Rosso, T, Rotondo, G, Sacquegna, T, Sanson, F, Santamato, V, Santoro, D, Sartori, V, Sasanelli, F, Savio, K, Serena, M, Silani, V, Silvestri, L, Simioni, V, Squintani, Gm, Suardelli, M, Tartagla, L, Terenghi, F, Terlizzi, E, Terzano, Mg, Tesser, F, Testa, L, Ticca, A, Ticozzi, N, Tiriticco, M, Tola, Mr, Tonietti, S, Trianni, G, Trojano, M, Trotta, F, Turatti, M, Ursino, E, Vanotti, A, Vercellino, M, Villani, A, Vitelli, E, Zambito Marsala, S, Zanette, G, Zarcone, D, Zimatore, G, Zoccolella, S., Magni E (ORCID:0000-0002-2235-2280), Benedetti, M. D., Pugliatti, M., Dalessandro, R., Beghi, E., Chio, A., Logroscino, G., Filippini, G., Galeotti, F., Massari, M., Santuccio, C., Raschetti R., Abruzzi L, Agazzi, E, Agostoni, E, Ambrogio, L, Amidei, S, Arbasino, C, Argentiero, V, Arnaboldi, M, Baldini, D, Barki, R, Bassi, P, Basso, F, Belcastro, V, Bellotti, M, Bersano, E, Besana, R, Bettoni, L, Bezzi, G, Bianconi, C, Bondavalli, M, Bonometti, A, Borghi, Am, Borsato, C, Bortolotto, S, Bottacchi, Ef, Bresolin, N, Bruno, S, Burlina, A, Cafasso, G, Callegarini, C, Calvo, A, Candeloro, E, Casano, A, Cattaneo, Si, Cavallo, R, Cheldi, A, Ciardo, G, Cirignotta, F, Clerici, Am, Clerici, R, Comi, G, Conti, R, Coppo, F, Covelli, V, Crespi, V, Currò Dossi, M, Curtò, Na, D'Adda, E, Dallocchio, C, D'Anna, S, De Massis, P, De Toni Franceschini, L, Di Vito, N, Didonè, G, Dileone, M, Donati, E, Dotta, M, Fazio, R, Federico, F, Ferrarese, C, Ferrazzini, F, Ferrero, B, Filosto, M, Frasson, E, Fusina, S, Galbussera, A, Gastaldo, E, Geda, C, Ghiglione, P, Giometto, B, Gionco, M, Giorgetti, A, Giussani, G, Gobbin, F, Grampa, G, Granieri, E, Greco, G, Guidetti, D, Guidi, C, Guidotti, M, Gusmaroli, G, Imperiale, D, Internò, S, Jann, S, La Spina, I, Leo, A, Leone, M, Leoni, S, Leotta, D, Lerario, R, Liotta, G, Livrea, P, Luda di Cortemiglio, E, Maggio, B, Magni, Eugenio, Magnoni, A, Maistrelli, J, Manca, D, Mandrioli, J, Manera, U, Marcello, N, Marchi, P, Marchini, C, Marconi, S, Mattioli, M, Mauro, A, Mazzaglia, G, Medici, D, Meineri, P, Meola, G, Micaglio, G, Michelucci, R, Michieli, G, Micieli, G, Minardi, C, Moglia, C, Monaco, S, Montanari, E, Moretto, G, Munerati, V, Mura, G, Mussutto, V, Nascimbene, C, Neri, W, Nichelli, P, Nobile-Orazio, E, Oddenino, E, Onorato, S, Padovani, A, Palermo, M, Papurello, Dm, Passarella, B, Pavesi, G, Penza, Mt, Perini, M, Perini, F, Perla, F, Perlotto, N, Perrone, P, Pignatta, P, Pisano, F, Poglio, F, Polo, A, Poloni, M, Porazzi, D, Pradotto, L, Previdi, P, Quatrale, R, Rasi, F, Ravasio, A, Ravetti, C, Repaci, M, Riccardi, T, Riguzzi, P, Rinaldi, R, Riva, M, Romeo, V, Romorini, A, Rosso, T, Rotondo, G, Sacquegna, T, Sanson, F, Santamato, V, Santoro, D, Sartori, V, Sasanelli, F, Savio, K, Serena, M, Silani, V, Silvestri, L, Simioni, V, Squintani, Gm, Suardelli, M, Tartagla, L, Terenghi, F, Terlizzi, E, Terzano, Mg, Tesser, F, Testa, L, Ticca, A, Ticozzi, N, Tiriticco, M, Tola, Mr, Tonietti, S, Trianni, G, Trojano, M, Trotta, F, Turatti, M, Ursino, E, Vanotti, A, Vercellino, M, Villani, A, Vitelli, E, Zambito Marsala, S, Zanette, G, Zarcone, D, Zimatore, G, Zoccolella, S., and Magni E (ORCID:0000-0002-2235-2280)
- Abstract
To assess Guillain-Barre syndrome (GBS) incidence we relied on the Italian Network for the study of GBS (ITANG) established in 2010 in 7 Italian regions to analyse the association between influenza vaccination and GBS. Methods: All individuals aged ≥18 years, presenting with clinical manifestations that suggested GBS according to the universally accepted Asbury's diagnostic criteria (1990) were prospectively notified to a centralised database by ITANG neurologists over the period October 1, 2010-September 30, 2011. Through a telephone survey, 9 trained interviewers followed up the cases to diagnosis and then for 1 year since hospital discharge. Validation of case reporting was performed with the support of administrative data in 5 regions. Results: We found 365 cases fulfilling the definition for GBS or one of its variants over 19,846,068 population ≥18 years of age, yielding an annual incidence rate of 1.84 per 100,000 (95% CI 1.65-2.03), 2.30 (95% CI 1.99-2.60) in men and 1.41 (95% CI 1.18-1.64) in women. A highly significant peak of incidence was observed in February 2011 as compared to reference month (September 2011, rate ratio 3.3:1, p < 0.01). Conclusions: In Italy, GBS incidence was among the highest reported in Europe and higher than previously observed in Italian studies.
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- 2015
14. The orthopaedic and traumatology scenario during Covid-19 outbreak in Italy: chronicles of a silent war
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Vincenzo Caiaffa, Biagio Moretti, Giuseppe Solarino, Fabrizio Quattrini, Vittorio Nappi, Raffaele Pezzella, Filippo Raggini, Raffaele Cioffi, Matteo Ghiara, Francesco Sanna, Placido Stissi, Enrico Vaienti, Alessandro Massè, Nathalie Bini, Alberto Castelli, Alberto Visigalli, Carmela Chinni, Luigi Murena, Emilio Luigi Mazza, Giulia Trovarelli, Giovanni Bove, Fabrizio Rivera, Guido Maritan, Gianluca Canton, Alberto Belluati, Michael Mazzacane, Francesco Benazzo, Antonio Medici, Pietro Maniscalco, Elisa Pala, Giovanni Vicenti, Angela Iuliano, Pietro Ruggieri, Francesco Pogliacomi, Agnese Puzzo, Bruno Di Maggio, Nunzia Garofalo, Alessandro Formica, Pietro Randelli, Stefano Marco Paolo Rossi, Marco Rosolani, Gabriele Scaravilli, Corrado Ciatti, Mario Mosconi, Andrea Angelini, Simone Ripanti, L Moretti, Loris Perticarini, Daniela Nonne, Andrea Colombelli, Alessandro Aprato, Fabio D'Angelo, Benazzo, F., Rossi, S. M. P., Maniscalco, P., Moretti, B., Vaienti, E., Ruggieri, P., Masse, A., Medici, A., Formica, A., Di Maggio, B., Caiaffa, V., Mosconi, M., Murena, L., D'Angelo, F., Belluati, A., Mazza, E. L., Rivera, F., Castelli, A., Ghiara, M., Rosolani, M., Cioffi, R., Pezzella, R., Scaravilli, G., Bove, G., Stissi, P., Mazzacane, M., Quattrini, F., Ciatti, C., Trovarelli, G., Pala, E., Angelini, A., Sanna, F., Nonne, D., Colombelli, A., Raggini, F., Puzzo, A., Canton, G., Maritan, G., Iuliano, A., Randelli, P., Solarino, G., Moretti, L., Vicenti, G., Garofalo, N., Nappi, V., Ripanti, S., Chinni, C., Pogliacomi, F., Visigalli, A., Bini, N., Aprato, A., and Perticarini, L.
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Traumatology ,Femoral Neck Fractures ,Disease Outbreaks ,0302 clinical medicine ,Trauma Centers ,Social mobility ,Medicine ,Orthopedic Procedures ,Orthopedics and Sports Medicine ,Viral ,Letter to the Editor ,Emergency Service ,030222 orthopedics ,Disease Outbreak ,Trauma Center ,Social situation ,Italy ,Covid-19 ,Orthopaedics ,Outbreak ,Pandemic ,Elective Surgical Procedures ,Emergency Service, Hospital ,Coronavirus Infections ,Human ,medicine.medical_specialty ,COVID-19 ,Humans ,Orthopedics ,SARS-CoV-2 ,Betacoronavirus ,Pandemics ,Pneumonia, Viral ,Coronavirus disease 2019 (COVID-19) ,Hospital ,03 medical and health sciences ,Orthopaedic trauma ,030203 arthritis & rheumatology ,Elective Surgical Procedure ,Betacoronaviru ,Coronavirus Infection ,business.industry ,General surgery ,Orthopedic ,Orthopaedic ,Pneumonia ,Orthopedic Procedure ,Orthopedic surgery ,Surgery ,business ,Trauma surgery - Abstract
Background: From February 21, the day of hospitalisation in ICU of the first diagnosed case of Covid-19, the social situation and the hospitals’ organisation throughout Italy dramatically changed. Methods: The CIO (Club Italiano dell’Osteosintesi) is an Italian society devoted to the study of traumatology that counts members spread in public and private hospitals throughout the country. Fifteen members of the CIO, Chairmen of 15 Orthopaedic and Trauma Units of level 1 or 2 trauma centres in Italy, have been involved in the study. They were asked to record data about surgical, outpatients clinics and ER activity from the 23rd of February to the 4th of April 2020. The data collected were compared with the data of the same timeframe of the previous year (2019). Results: Comparing with last year, overall outpatient activity reduced up to 75%, overall Emergency Room (ER) trauma consultations up to 71%, elective surgical activity reduced up to 100% within two weeks and trauma surgery excluding femoral neck fractures up to 50%. The surgical treatment of femoral neck fractures showed a stable reduction from 15 to 20% without a significant variation during the timeframe. Conclusions: Covid-19 outbreak showed a tremendous impact on all orthopaedic trauma activities throughout the country except for the surgical treatment of femoral neck fractures, which, although reduced, did not change in percentage within the analysed timeframe.
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- 2020
15. Sound-Induced Flash Illusions Support Cortex Hyperexcitability in Fibromyalgia
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Vincenzo Di Stefano, Salvatore Iacono, Andrea Gagliardo, Bruna Maggio, Giuliana Guggino, Massimo Gangitano, Roberto Monastero, Vito Renato Maggio, Nadia Bolognini, Filippo Brighina, Di Stefano, Vincenzo, Iacono, Salvatore, Gagliardo, Andrea, Maggio, Bruna, Guggino, Giuliana, Gangitano, Massimo, Monastero, Roberto, Maggio, Vito Renato, Bolognini, Nadia, Brighina, Filippo, Di Stefano, V, Iacono, S, Gagliardo, A, Maggio, B, Guggino, G, Gangitano, M, Monastero, R, Maggio, V, Bolognini, N, and Brighina, F
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Adult ,Fibromyalgia ,Article Subject ,Migraine Disorders ,Infant, Newborn ,Anxiety ,Middle Aged ,Illusions ,Anesthesiology and Pain Medicine ,Neurology ,Illusion ,Migraine Disorder ,Acoustic Stimulation ,Auditory Perception ,Humans ,Settore MED/26 - Neurologia ,Female ,Photic Stimulation ,Human - Abstract
Objectives. Fibromyalgia (FM) is characterized by spontaneous chronic widespread pain in combination with hyperalgesia to pressure stimuli. Sound-induced flash illusions (SIFIs) reflect cross-modal interactions between senses allowing to assess a visual cortical hoerexcitability (VCH) by evaluating the fission and fusion illusions disruption. The aims of the present study were to explore whether SIFIs are perceived differently in patients with fibromyalgia as compared to healthy controls (HCs) and how migraine affects fission and fusion illusions in fibromyalgia. Methods. A single flash (F) accompanied by 0 to 4 beeps (B) was presented to induce the fission illusion while multiple flash (i.e., 2 to 4) accompanied by 0 or 1 beep was presented to induce fusion illusion. The mean number of perceived flashes in fission and fusion illusion trials was compared between the groups (i.e., FM, FM with migraine, and HCs) using repeated-measures analysis of variance. Medication history was recorded along with the administration of Fibromyalgia Impact Questionnaire and Hospital Anxiety and Depression scales. Results. Twenty-four patients with FM (mean age 51, 2 ± 10, 6 years; 22 females), seventeen patients with FM and migraine without aura (mean age 47.8 ± 11.4 years; 16 females; 13 chronic, 4 episodic migraine), and forty-one age- and sex-matched HCs (mean age 47.3 ± 6.9 years; 34 females) participated in the study. Fission and fusion illusory effects were detected in all the participants. However, in FM patients, the fission illusion was reduced and almost abolished as compared to HCs (1F1B, p = 0.02 ; 1F2B, p < 0.0001 ; 1F3B, p < 0.0001 ; 1F4B, p = 0.0001 ), while there were no differences between groups in fusion trials. Migraine did not affect the fission and the fusion illusions. Conclusion. Results from this study confirm that patients with FM have a VCH suggesting that the pathological changes in cortical excitability might have important roles in the pathophysiology of FM. SIFI represents a noninvasive behavioral tool for the exploration of cross-sensory functional interplay.
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- 2022
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16. Frequency and Correlates of Mild Cognitive Impairment in Myasthenia Gravis
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Salvatore Iacono, Vincenzo Di Stefano, Vanessa Costa, Giuseppe Schirò, Antonino Lupica, Bruna Maggio, Davide Norata, Antonia Pignolo, Filippo Brighina, Roberto Monastero, Iacono S., Di Stefano V., Costa V., Schiro G., Lupica A., Maggio B., Norata D., Pignolo A., Brighina F., and Monastero R.
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myasthenia gravis ,depressive symptoms ,General Neuroscience ,prevalence ,Settore MED/26 - Neurologia ,sleep disorders ,neuropsychological testing ,cognitive impairment - Abstract
Background: Antibodies against acetylcholine receptors (AChRs) can also target nicotinic AChRs that are present throughout the central nervous system, thus leading to cognitive dysfunctions in patients with myasthenia gravis (MG). However, the presence of cognitive impairment in MG is controversial, and the factors that may influence this risk are almost completely unknown. In this study, the frequency of mild cognitive impairment (MCI) in MG, as well as the clinical, immunological, and behavioral correlates of MCI in MG were evaluated. Methods: A total of 52 patients with MG underwent a comprehensive assessment including motor and functional scales, serological testing, and neuropsychological and behavioral evaluation. Results: The frequency of MCI was 53.8%, and the most impaired cognitive domains were, in order, visuoconstructive/visuospatial skills, memory, and attention. After multivariate analysis, only pyridostigmine use was inversely associated with the presence of MCI, while a trend toward a positive association between MCI and disease severity and arms/legs hyposthenia was found. Correlation analyses showed that daily doses of prednisone and azathioprine significantly correlated with depressive symptomatology, while disease severity significantly correlated with depressive symptomatology and sleep disturbance. Conclusions: The presence of MCI is rather frequent in MG and is characterized by multidomain amnestic impairment. Such preliminary data need further confirmation on larger case series.
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- 2023
17. New complex polycyclic compounds: Synthesis, antiproliferative activity and mechanism of action
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Ernest Hamel, Benedetta Maggio, Maria Valeria Raimondi, Marianna Lauricella, Demetrio Raffa, Giuseppe Daidone, Antonella D'Anneo, Fabiana Plescia, Daidone G., D'Anneo A., Raimondi M.V., Raffa D., Hamel E., Plescia F., Lauricella M., and Maggio B.
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Stereochemistry ,O-glycoconjugate polycyclic compounds ,Apoptosis ,Antiproliferative activity ,Crystallography, X-Ray ,01 natural sciences ,Biochemistry ,Structure-Activity Relationship ,Breast cancer cell line ,Cell Line, Tumor ,Drug Discovery ,medicine ,Autophagy ,MDA-MB231 breast cancer cells ,Humans ,Polycyclic Compounds ,Cytotoxicity ,Molecular Biology ,Cell Proliferation ,010405 organic chemistry ,Hydrogen bond ,Chemistry ,Organic Chemistry ,Hydrogen Bonding ,In vitro ,0104 chemical sciences ,Partition coefficient ,010404 medicinal & biomolecular chemistry ,Mechanism of action ,Pyrazolo[3,4-b]pyrazolo[3′,4′:2,3]azepino[4,5-f]azocine ,medicine.symptom - Abstract
Polycyclic or O-glycoconiugate polycyclic compounds 1a-g were previously tested for their in vitro antiproliferative activity. In this series of compounds, activity increases as log P decreases. Specifically, compounds 1d and 1g showed lower log P values together with the best antiproliferative profiles. With the aim of extending our understanding of the structure–activity relationship (SAR) of this class of compounds, we prepared new polycyclic derivatives 2a-c, which bear on each of the two phenyl rings hydrophilic substituents (OH, SO2NH2 or NHCOCH3). These substituents are able to form hydrogen bonds and to decrease the partition coefficient value as compared with compound 1d. Compound 2a was slightly more active than 1d, while 2b and 2c had antiproliferative activity comparable to that of 1d. Finally, the role of the two phenyl groups of polycycle derivatives 1 was also investigated. The analog 3, which bears two methyls instead of the two phenyls had a lower log P value (2.94 ± 1.22) than all the other compounds, but it had negligible antiproliferative activity at 10 µM. The analysis of the most active derivative 2a revealed a significant antiproliferative activity against the triple-negative breast cancer cell line MDA-MB231. After a 24 h treatment, an autophagic process was activated, as demonstrated by an increase in monodansylcadaverine-positive cells as well as by the appearance of the autophagic markers Beclin and LC3II. Prolonging the treatment to 48 h, 2a caused cytotoxicity through the activation of caspase-dependent apoptosis.
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- 2020
18. Synthesis and biofilm formation reduction of pyrazole-4-carboxamide derivatives in some Staphylococcus aureus strains
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Maria Grazia Cusimano, Fabiana Plescia, Demetrio Raffa, Domenico Schillaci, Giuseppe Daidone, Maria Valeria Raimondi, Benedetta Maggio, Barbara Manachini, Stella Cascioferro, Cascioferro, S., Maggio, B., Raffa, D., Raimondi, M., Cusimano, M., Schillaci, D., Manachini, B., Plescia, F., and Daidone, G.
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0301 basic medicine ,Staphylococcus aureus ,medicine.drug_class ,030106 microbiology ,Carboxamide ,Moths ,N-phenyl-1H-pyrazole-4-carboxamide ,Pyrazole ,Settore BIO/19 - Microbiologia Generale ,medicine.disease_cause ,01 natural sciences ,Microbiology ,Structure-Activity Relationship ,03 medical and health sciences ,chemistry.chemical_compound ,Drug Discovery ,Inhibition of biofilm formation ,medicine ,Animals ,IC50 ,Pharmacology ,Wax ,Virulence ,biology ,010405 organic chemistry ,Drug Discovery3003 Pharmaceutical Science ,Anti-virulence ,Organic Chemistry ,Biofilm ,S. aureu ,General Medicine ,Staphylococcal Infections ,biology.organism_classification ,Settore CHIM/08 - Chimica Farmaceutica ,Anti-Bacterial Agents ,0104 chemical sciences ,Galleria mellonella ,Hydrazines ,Settore AGR/11 - Entomologia Generale E Applicata ,chemistry ,Biofilms ,Larva ,visual_art ,Wax moth larva model ,visual_art.visual_art_medium ,Pyrazoles ,Lead compound - Abstract
The ability of several N-phenyl-1H-pyrazole-4-carboxamide derivatives and other pyrazoles opportunely modified at the positions 3, 4 and 5, to reduce the formation of the biofilm in some Staphylococcus aureus strains (ATCC 29213, ATCC 25923 and ATCC 6538) were investigated. All the tested compounds were able, although to a different extent, to reduce the biofilm formation of the three bacterial strains considered. Among these, the 1-(2,5-dichlorophenyl)-5-methyl-N-phenyl-1H-pyrazole-4-carboxamide 14 resulted as the best inhibitor of biofilm formation showing an IC50 ranging from 2.3 to 32 μM, against all the three strains of S. aureus. Compound 14 also shows a good protective effect in vivo by improving the survival of wax moth larva (Galleria mellonella) infected with S. aureus ATCC 29213. These findings indicate that 14d is a potential lead compound for the development of new anti-virulence agents against S. aureus infections.
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- 2016
19. Synthesis and antiproliferative activity of a natural like glycoconjugate polycyclic compound
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Antonella D'Anneo, Marie-Christine Scherrmann, Marianna Lauricella, Demetrio Raffa, Fabiana Plescia, Benedetta Maggio, Nicolò Prosa, Maria Valeria Raimondi, Giuseppe Daidone, Maggio, B., Raimondi, M., Raffa, D., Plescia, F., Scherrmann, M., Prosa, N., Lauricella, M., D'Anneo, A., and Daidone, G.
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0301 basic medicine ,Cell cycle checkpoint ,Cell Survival ,Receptor, ErbB-2 ,Stereochemistry ,Glycoconjugate ,Antineoplastic Agents ,Antiproliferative activity ,Chemistry Techniques, Synthetic ,03 medical and health sciences ,0302 clinical medicine ,Cyclin-dependent kinase ,Cell Line, Tumor ,Drug Discovery ,Humans ,Polycyclic Compounds ,MDA-MB231 ,Cyclin B1 ,Cell Proliferation ,Cyclin ,Pharmacology ,chemistry.chemical_classification ,Biological Products ,Cyclin-dependent kinase 1 ,G2/M phase arrest ,p21WAF1 inhibitor ,biology ,Chemistry ,Kinase ,Drug Discovery3003 Pharmaceutical Science ,O-glycoconjugate polycyclic compound ,Organic Chemistry ,General Medicine ,Molecular biology ,G2 Phase Cell Cycle Checkpoints ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Cell culture ,Pyrazolo[3,4-b]pyrazolo[3′,4′:2,3]azepino[4,5-f]azocine ,Drug Design ,030220 oncology & carcinogenesis ,biology.protein ,M Phase Cell Cycle Checkpoints ,Receptors, Progesterone ,Glycoconjugates - Abstract
A natural like O -glycoconjugate polycyclic compound 4 was obtained by a multistep procedure starting from N -(3-methyl-1-(4-nitrophenyl)-1 H -pyrazol-5-yl)acetamide. The glycosyl derivative 4 showed antiproliferative activity against all the tumoral cell lines of the NCI panel in the range 0.47–5.43 μ M. Cytofluorimetric analysis performed on MDA-MB231, a very aggressive breast cancer cell line, which does not express estrogen, progesterone and HER-2/neu receptors, showed that 4 is able to induce prolonged cell cycle arrest at G2/M phase and morphological signs of differentiation. These events are correlated with down-regulation of both cyclin B1 and cdc2, the cyclins involved in G2/M transition, as well as up-regulation of cyclin-dependent kinase (CDK) inhibitor p21 Cip1/Waf1.
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- 2016
20. A new class of phenylhydrazinylidene derivatives as inhibitors of Staphylococcus aureus biofilm formation
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Maria Grazia Cusimano, Benedetta Maggio, Ainars Leonchiks, Giuseppe Daidone, Barbara Manachini, Stella Cascioferro, Maria Valeria Raimondi, Demetrio Raffa, Domenico Schillaci, Cascioferro, S., Maggio, B., Raffa, D., Raimondi, M., Cusimano, M., Schillaci, D., Manachini, B., Leonchiks, A., and Daidone, G.
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0301 basic medicine ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Bacterial adhesion ,Antibiofilm agent ,Settore BIO/19 - Microbiologia Generale ,medicine.disease_cause ,Microbiology ,Antivirulence agent ,03 medical and health sciences ,Antibiotic resistance ,In vivo ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,biology ,Chemistry ,Sortase A ,Organic Chemistry ,Biofilm ,Phenylhydrazinylidene derivative ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Settore CHIM/08 - Chimica Farmaceutica ,Galleria mellonella ,Settore AGR/11 - Entomologia Generale E Applicata ,030104 developmental biology ,Mechanism of action ,Biochemistry ,Staphylococcus aureus ,Pharmacology, Toxicology and Pharmaceutics (all) ,medicine.symptom - Abstract
In the struggle against the emergence of the antibiotic resistance, new molecules targeting biofilm formation could be useful as adjuvant of conventional antibiotics. This study focused on a new class of 2-phenylhydrazinylidene derivatives as antivirulence agents. The compound 12e showed interesting activities against biofilm formation of all tested Staphylococcus aureus strains with IC50 ranging from 1.7 to 43 µM; compounds 12f and 13a resulted strong inhibitors of S. aureus ATCC 6538 and ATCC 29213 biofilm formation with IC50 of 0.9 and 0.8 µM, respectively. A preliminary study on the mechanism of action was carried on evaluating the inhibition of sortase A transpeptidase. Compound 12e resulted not to be toxic at 1 mg/ml by using an in vivo model (the wax moth larva model, Galleria mellonella).
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- 2016
21. Synthesis and antiproliferative activity of 3-(2-chloroethyl)-5-methyl-6-phenyl-8-(trifluoromethyl)-5,6-dihydropyrazolo[3,4-f][1,2,3,5]tetrazepin-4-(3H)-one
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Gabriella Cancemi, Manlio Tolomeo, Maria Valeria Raimondi, Giuseppe Daidone, Fabiana Plescia, Benedetta Maggio, Demetrio Raffa, Stella Cascioferro, Stefania Grimaudo, Maggio, B., Raimondi, M., Raffa, D., Plescia, F., Cascioferro, S., Cancemi, G., Tolomeo, M., Grimaudo, S., and Daidone, G.
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Methyltransferase ,Stereochemistry ,HL60 ,Antineoplastic Agents ,Apoptosis ,HL-60 Cells ,Structure-Activity Relationship ,chemistry.chemical_compound ,Drug Discovery ,Humans ,Structure–activity relationship ,Cell Proliferation ,Pharmacology ,Trifluoromethyl ,Dose-Response Relationship, Drug ,Molecular Structure ,Chemistry ,Cell growth ,Cell Cycle ,Organic Chemistry ,Azepines ,General Medicine ,Cell cycle ,Settore CHIM/08 - Chimica Farmaceutica ,1,2,3,5-Tetrazepinones, pyrazolo[3,4-f][1,2,3,5]-tetrazepinones, drug resistance, apoptosis, antiproliferative activity ,Cell culture ,Pyrazoles ,Drug Screening Assays, Antitumor ,K562 Cells - Abstract
Based on the encouraging results found for 3,5-dimethyl-6-phenyl-8-(trifluoromethyl)-5,6-dihydropyrazolo[3,4-f][1,2,3,5]tetrazepin-4-(3H)-one 7 previously tested by us, as well as the consideration that heterocycle fused tetrazepinones bearing the 2-chloroethyl substituent show a better cytotoxic profile than temozolomide and mitozolomide against human cancer cell lines which express the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT), in this paper we report the multistep synthesis and the biological study of 3-(2-cloroethyl)-5-methyl-6-phenyl-8-(trifluoromethyl)-5,6-dihydropyrazolo[3,4-f][1,2,3,5]tetrazepin-4-(3H)-one 10. Like compound 7, it was active on P-glycoprotein expressing cells (MDR) HL60 and on K562 cell line that are resistant to apoptosis induced by different stimuli, showing GI50 values of 14 and 18 µM respectively. As an antiproliferative agent against the above cells compound 10 was about 2.2 times more active than compound 7. Compound 10 was also tested against WiDR cells which are overexpressing the DNA repair protein MGMT, showing a GI50 value of 2.3 µM. Finally, concerning the effect on cell cycle we observed an evident difference between compounds 7 and 10. In fact, compound 7 induces a block of cell cycle in G0-G1, therefore acting as phase-specific drug, in contrast, compound 10 is a not phase-specific agent. Both the compounds are able to increase the apoptotic sub G0-G1 peak of cell cycle.
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- 2015
22. Recent discoveries of anticancer flavonoids
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Fabiana Plescia, Giuseppe Daidone, Benedetta Maggio, Maria Valeria Raimondi, Demetrio Raffa, Raffa, D., Maggio, B., Raimondi, M., Plescia, F., and Daidone, G.
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0301 basic medicine ,Computational biology ,Chemoprevention ,03 medical and health sciences ,Structure-Activity Relationship ,0302 clinical medicine ,Neoplasms ,Drug Discovery ,Animals ,Humans ,heterocyclic compounds ,Benzopyrans ,Chemoprotection ,Pharmacology ,Flavonoids ,Chemistry ,Drug Discovery3003 Pharmaceutical Science ,fungi ,Organic Chemistry ,food and beverages ,General Medicine ,Plants ,Antineoplastic Agents, Phytogenic ,030104 developmental biology ,030220 oncology & carcinogenesis ,Flavonoid ,Bioactive compound ,Chemotherapeutic agent - Abstract
In this review we report the recent advances in anticancer activity of the family of natural occurring flavonoids, covering the time span of the last five years. The bibliographic data will be grouped, on the basis of biological information, in two great categories: reports in which the extract plants bioactivity is reported and the identification of each flavonoid is present or not, and reports in which the anticancer activity is attributable to purified and identified flavonoids from plants. Wherever possible, the targets and mechanisms of action as well as the structure-activity relationships of the molecules will be reported. Also, in the review it was thoroughly investigated the recent discovery on flavonoids containing the 2-phenyl-4H-chromen-4-one system even if some examples of unusual flavonoids, bearing a non-aromatic B-ring or other ring condensed to the base structure are reported.
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- 2017
23. Anthranilamide-based 2-phenylcyclopropane-1-carboxamides, 1,1'-biphenyl-4-carboxamides and 1,1'-biphenyl-2-carboxamides: Synthesis biological evaluation and mechanism of action
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Giuseppe Daidone, Fabiana Plescia, Antonella D'Anneo, Demetrio Raffa, Benedetta Maggio, Maria Valeria Raimondi, Marianna Lauricella, Raffa, D., Plescia, F., Maggio, B., Raimondi, M., D'Anneo, A., Lauricella, M., and Daidone, G.
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0301 basic medicine ,G2 Phase ,2-Phenylcyclopropane-1-carboxamides, 1,1’-biphenyl-4-carboxamides, 1,1’-biphenyl-2-carboxamides, G2/M arrest, Phospho-ATM and gH2AX increase ,DNA Repair ,DNA repair ,Stereochemistry ,Antineoplastic Agents ,Apoptosis ,Chloride ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Settore BIO/10 - Biochimica ,Drug Discovery ,medicine ,Cytotoxic T cell ,Humans ,ortho-Aminobenzoates ,Mode of action ,Cell Proliferation ,Pharmacology ,Chemistry ,Organic Chemistry ,General Medicine ,Cell Cycle Checkpoints ,Cell cycle ,Settore CHIM/08 - Chimica Farmaceutica ,030104 developmental biology ,Mechanism of action ,030220 oncology & carcinogenesis ,medicine.symptom ,K562 Cells ,DNA ,medicine.drug ,DNA Damage - Abstract
Several anthranilamide-based 2-phenylcyclopropane-1-carboxamides 13a-f, 1,1’-biphenyl-4-carboxamides 14a-f and 1,1’-biphenyl-2-carboxamides 17a-f were obtained by a multistep procedure starting from the (1S,2S)-2-phenylcyclopropane-1-carbonyl chloride 11, the 1,1'-biphenyl-4-carbonyl chloride 12 or the 1,1'-biphenyl-2-carbonyl chloride 16 with the appropriate anthranilamide derivative 10a-f. Derivatives 13a-f, 14a-f and 17a-f showed antiproliferative activity against human leukemia K562 cells. Among these derivatives 13b, 14b and 17b exerted a particular cytotoxic effect on tumor cells. Derivative 17b showed a better antitumoral effect on K562 cells than 13b and 14b. Analyses performed to explore 17b mode of action revealed that it induced an arrest in G2/M phase of cell cycle which was consequent to DNA lesions as demonstrated by the increase in phospho-ATM and γH2AX, two known markers of DNA repair response system. The effect of 17b was also related to ROS generation, activation of JNK and induction of caspase-3 dependent apoptosis.
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- 2017
24. Derivati policiclici con struttura complessa dotati di attività antiproliferativa
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MAGGIO, Benedetta, RAIMONDI, Maria Valeria, RAFFA, Demetrio, PLESCIA, Fabiana, Schermann, MC, Prosa, N, LAURICELLA, Marianna, D'ANNEO, Antonella, DAIDONE, Giuseppe, Maggio, B, Raimondi, MV, Raffa, D, Plescia, F, Schermann, MC, Prosa, N, Lauricella, M, D'Anneo, A, and Daidone, G
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Settore BIO/10 - Biochimica ,derivati policiclici, attivita antitumorale, ciclo cellulare ,Settore CHIM/08 - Chimica Farmaceutica - Abstract
Sono stati sintetizzati composti a struttura policiclica complessa. Tutti i composti sintetizzati sono stati saggiati dall'NCI (USA) su un panel di 60 linee cellulari tumorali umane. Sula base dei risultati ottenuti è stato possibile effettuare uno studio SAR che ha evidenziato come la presenza di gruppi idrofili è necessaria ai fini dell'attività antitumorale. E' stato inoltre studiato il meccanismo d'azione di queste molecole. E' stato notato un arresto delle cellule MDA-MB231 in fase G0/G1 correlato con la defosforilazione ed attivazione, indotta dai composti, della proteina pRb. Il meccanismo comunque varia arrestando il ciclo cellulare in fase G2/M quando per effetto della glicosilazione del derivato.
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- 2017
25. Discovery of a new class of sortase a transpeptidase inhibitors to tackle gram-positive pathogens: 2-(2-phenylhydrazinylidene)alkanoic acids and related derivatives
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Fabiana Plescia, Demetrio Raffa, Dmitrijs Zhulenkovs, Maria Grazia Cusimano, Benedetta Maggio, Ainars Leonchiks, Giuseppe Daidone, Domenico Schillaci, Stella Cascioferro, Maria Valeria Raimondi, Livia Basile, Maggio, B., Raffa, D., Raimondi, M., Cascioferro, S., Plescia, F., Schillaci, D., Cusimano, M., Leonchiks, A., Zhulenkovs, D., Basile, L., and Daidone, G.
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sortase A ,biofilms ,2-(2-phenylhydrazinylidene)alkanoic acid derivatives ,FRET ,0301 basic medicine ,Staphylococcus aureus ,Stereochemistry ,Pharmaceutical Science ,Related derivatives ,medicine.disease_cause ,Settore BIO/19 - Microbiologia Generale ,01 natural sciences ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,Inhibitory Concentration 50 ,03 medical and health sciences ,chemistry.chemical_compound ,2-(2-phenylhydrazinylidene)alkanoic acid derivative ,Anti-Infective Agents ,Bacterial Proteins ,lcsh:Organic chemistry ,Staphylococcus epidermidis ,Amide ,Drug Discovery ,medicine ,Enzyme Inhibitors ,Physical and Theoretical Chemistry ,IC50 ,Gram ,biology ,010405 organic chemistry ,Chemistry ,Biofilm ,Sortase A ,Organic Chemistry ,Aminoacyltransferases ,biology.organism_classification ,Settore CHIM/08 - Chimica Farmaceutica ,Phenylhydrazines ,0104 chemical sciences ,Cysteine Endopeptidases ,030104 developmental biology ,Chemistry (miscellaneous) ,Molecular Medicine - Abstract
A FRET-based random screening assay was used to generate hit compounds as sortase A inhibitors that allowed us to identify ethyl 3-oxo-2-(2-phenylhydrazinylidene)butanoate as an example of a new class of sortase A inhibitors. Other analogues were generated by changing the ethoxycarbonyl function for a carboxy, cyano or amide group, or introducing substituents in the phenyl ring of the ester and acid derivatives. The most active derivative found was 3-oxo-2-(2-(3,4dichlorophenyl)hydrazinylidene)butanoic acid (2b), showing an IC50 value of 50 µM. For a preliminary assessment of their antivirulence properties the new derivatives were tested for their antibiofilm activity. The most active compound resulted 2a, which showed inhibition of about 60% against S. aureus ATCC 29213, S. aureus ATCC 25923, S. aureus ATCC 6538 and S. epidermidis RP62A at a screening concentration of 100 µM.
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- 2016
26. Isoxazolo[3,4-d]pyridazin-7(6H)-one derivatives endowed with anti-proliferative Activity
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Maggio, Benedetta, Cancemi, G., Demetrio Raffa, Maria Valeria Raimondi, Plescia, Fabiana, Anneo, Antonella D., Lauricella, Marianna, Giampaolo Barone, Riccardo Bonsignore, Giuseppe Daidone, Maggio, B, Cancemi, G, Raffa, D, Raimondi, MV, Plescia,F, D’Anneo, A, Lauricella, M, Barone, G, Bonsignore, R, and Daidone, G.
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anti-proliferative activity ,Isoxazolo[3,4-d]pyridazin-7(6H)-one derivative - Abstract
Isoxazolo[3,4-d]pyridazin-7(6H)-one derivatives endowed with antiproliferative Activity B. Maggio1, G. Cancemi1, D. Raffa1, M. V. Raimondi1, F. Plescia1, A. D’Anneo2,M. Lauricella3, G. Barone4, R. Bonsignore4, G. Daidone1 1. Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, Medicinal Chemistry and Pharmaceutical Technologies Section, University of Palermo, ViaArchirafi 32, 90123, Palermo, Italy 2. Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, Laboratory of Biochemistry, University of Palermo. 3.Department of Experimental Biomedicine and Clinical Neurosciences, Laboratory of Biochemistry, University of Palermo. 4. Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo. benedetta.maggio@unipa.it A screening carried out by NCI (Bethesda, USA) on compounds available in our laboratory,in order to ascertain their antiproliferative activity against a panel of 60 human tumor cell lines, allowed to discovery the 3,4-diphenylisoxazolo[3,4-d]pyridazin-7(6H)-one 1a [1] as an hit compound, often showing GI50values at sub-micromolar level. We synthesized some analogs of 1a, i.e.1b-gand other derivatives in which the NHgroup is variably alkylated, with the aim to obtain more active compounds as well as to perform structure-activity relationship(SAR) studies. We obtained a quite active antiproliferative compound, the 3,4-di-p-tolylisoxazolo[3,4-d]pyridazin-7(6H)- one 1d, and verified the importance for the antiproliferative activity of the aril, and not alkyl, groups linked to the isoxazolo-pyridazinone moiety. Studies performed on the cell cycle progression and on some cellular target (ATM, procaspase-2 proteins and H2AX histone) demonstrated that 1d produces an increase of the cell population in pre-G0/G1 and induces cellular death by apoptosis, damaging the DNA by double strand breaks. UV-vis titration and viscosity measurement showed that the compound is able to give an interaction with the B-DNA. 1. Renzi G., Dal Piaz V. (2004)Gazz. Chim. It.95: 1478–1491
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- 2016
27. SINTESI ED ATTIVITÀ ANTIPROLIFERATIVA DI NUOVI DERIVATI A STRUTTURA TETRAZEPINONICA
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RAIMONDI, Maria Valeria, PLESCIA, Fabiana, INGARRA, Angela Maria, GRIMAUDO, Stefania, Tolomeo, M, MAGGIO, Benedetta, Raimondi, MV, Plescia, F, Ingarra, AM, Grimaudo, S, Tolomeo, M, and Maggio, B
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Attività antitumorale ,Settore BIO/13 - Biologia Applicata ,Temozolomide ,Tetrazepinoni ,Settore CHIM/08 - Chimica Farmaceutica ,Multi-farmaco resistenza - Abstract
Sintesi di due nuovi composti a struttura tetrazepinonica, analoghi della Temozolomide. I due nuovi composti hanno mostrato una interessante attività antiproliferativa nei confronti di linee cellulari tumorali esprimenti l'enzima MGMT
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- 2016
28. Sortase A Inhibitors: Recent Advances and Future Perspectives
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Maria Valeria Raimondi, Benedetta Maggio, Domenico Schillaci, Stella Cascioferro, Demetrio Raffa, Giuseppe Daidone, Cascioferro, S., Raffa, D., Maggio, B., Raimondi, M., Schillaci, D., and Daidone, G.
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Models, Molecular ,Staphylococcus aureus ,Rhodanine ,Protein Conformation ,Virulence ,Adamantane ,medicine.disease_cause ,Staphylococcal infections ,Settore BIO/19 - Microbiologia Generale ,Benzoates ,Bacterial Adhesion ,Sortase A inhibitors, review, future perspective ,Microbiology ,Small Molecule Libraries ,Bacterial Proteins ,In vivo ,Drug Discovery ,Nitriles ,medicine ,Animals ,Humans ,Enzyme Inhibitors ,chemistry.chemical_classification ,Lipid II ,biology ,Thiones ,Staphylococcal Infections ,biology.organism_classification ,medicine.disease ,Aminoacyltransferases ,Settore CHIM/08 - Chimica Farmaceutica ,Amino acid ,Anti-Bacterial Agents ,Cysteine Endopeptidases ,Thiazoles ,Biochemistry ,chemistry ,Sortase A ,Molecular Medicine ,Bacteria ,Carbolines - Abstract
Here, we describe the most promising small synthetic organic compounds that act as potent Sortase A inhibitors and cater the potential to be developed as antivirulence drugs. Sortase A is a polypeptide of 206 amino acids, which catalyzes two sequential reactions: (i) thioesterification and (ii) transpeptidation. Sortase A is involved in the process of bacterial adhesion by anchoring LPXTG-containing proteins to lipid II. Sortase A inhibitors do not affect bacterial growth, but they restrain the virulence of pathogenic bacterial strains, thereby preventing infections caused by Staphylococcus aureus or other Gram-positive bacteria. The efficacy of the most promising inhibitors needs to be comprehensively evaluated in in vivo models of infection, in order to select compounds eligible for the treatment of bacterial infections in humans.
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- 2015
29. Synthesis, antiproliferative activity and possible mechanism of action of novel 2-acetamidobenzamides bearing the 2-phenoxy functionality
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Maria Grazia Cusimano, Stella Cascioferro, Benedetta Maggio, Gabriella Cancemi, Ernest Hamel, Fabiana Plescia, Maria Valeria Raimondi, Antonella D'Anneo, Ruoli Bai, Giuseppe Daidone, Demetrio Raffa, Marianna Lauricella, Raffa D, Maggio B, Plescia, F, Cascioferro, S, Raimondi, MV, Cancemi, G, D'Anneo, A, Lauricella, M, Cusimano, MG, Bai, R, Hamel, E, and Daidone,G
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Clinical Biochemistry ,Cell ,Pharmaceutical Science ,Antineoplastic Agents ,Apoptosis ,Antiproliferative activity ,Pharmacology ,G0/G1 arrest ,Biochemistry ,Article ,2-(2-Phenoxyacetamido)benzamide ,Antineoplastic Agent ,Structure-Activity Relationship ,Benzamide ,Settore BIO/10 - Biochimica ,Cell Line, Tumor ,Drug Discovery ,G1 Phase Cell Cycle Checkpoint ,K562 Cell ,medicine ,Humans ,Molecular Biology ,Cell Proliferation ,Cell growth ,Chemistry ,Drug Discovery3003 Pharmaceutical Science ,Organic Chemistry ,Apoptosi ,Cell cycle ,medicine.disease ,Caspase ,Settore CHIM/08 - Chimica Farmaceutica ,G1 Phase Cell Cycle Checkpoints ,Leukemia ,medicine.anatomical_structure ,Microscopy, Fluorescence ,Cell culture ,Caspases ,Benzamides ,Molecular Medicine ,Drug Screening Assays, Antitumor ,K562 Cells ,Pro-caspase 3 ,Human ,K562 cells ,Chronic myelogenous leukemia - Abstract
Several new 2-(2-phenoxyacetamido)benzamides 17a-v, 21 and 22 were synthesized by stirring in pyridine the acid chlorides 16a-e and the appropriate5-R-4-R1-2-aminobenzamide 15a-e and initially evaluated in vitro for antiproliferative activity against the K562 (human chronic myelogenous leukemia) cell line. Some of synthesized compounds were evaluated for their in vitro antiproliferative activity against the full NCI tumor cell line panel derived from nine clinically isolated cancer types (leukemia, non-small cell lung, colon, CNS, melanoma, ovarian, renal, prostate and breast). The most active compounds caused an arrest of K562 cells in the G0-G1 phase of cell cycle and induction of apoptosis, which was mediated by caspase activation.
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- 2015
30. Recent advanced in bioactive systems containing pyrazole fused with a five membered heterocycle
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Giuseppe Daidone, Demetrio Raffa, Fabiana Plescia, Stella Cascioferro, Benedetta Maggio, Maria Valeria Raimondi, Gabriella Cancemi, Raffa, D, Maggio, B, Raimondi, MV, Cascioferro, SM, Plescia, F, Cancemi, G, and Daidone, G
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Pharmacology ,Stereochemistry ,Organic Chemistry ,pyrazole fused with a five membered heterocycle, bioactive system ,General Medicine ,Pyrazole ,Ring (chemistry) ,Settore CHIM/08 - Chimica Farmaceutica ,chemistry.chemical_compound ,Oxygen atom ,chemistry ,Therapeutic Area ,Drug Discovery ,Animals ,Humans ,Pyrazoles ,Molecule - Abstract
In this review we report the recent advances in bioactive system containing pyrazole fused with a five membered heterocycle, covering the time span of the last decade. All of them are represented around the common structure of the pyrazole ring fused with another five membered heterocycle containing the nitrogen, sulfur and oxygen atoms in all their possible combinations. The classification we have used is based in terms of the therapeutic area providing, when possible, some general conclusions on the targets and mechanisms of action as well as the structure-activity relationships of the molecules.
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- 2015
31. Pharmaceutical potential of synthetic and natural pyrrolomycins
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Benedetta Maggio, Stella Cascioferro, Demetrio Raffa, Maria Grazia Cusimano, Maria Valeria Raimondi, Domenico Schillaci, Giuseppe Daidone, Cascioferro, S., Raimondi, M., Cusimano, M., Raffa, D., Maggio, B., Daidone, G., and Schillaci, D.
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Pyoluteorin ,antibiotic resistance ,medicine.drug_class ,Antibiotics ,Pharmaceutical Science ,Microbial Sensitivity Tests ,Review ,Pharmacology ,Antibiofilm agent ,pyrrolomycin ,Settore BIO/19 - Microbiologia Generale ,Analytical Chemistry ,lcsh:QD241-441 ,Antibiotic resistance ,lcsh:Organic chemistry ,Drug Discovery ,Drug Resistance, Bacterial ,Medicine ,Animals ,Humans ,Pyrroles ,Clinical efficacy ,Physical and Theoretical Chemistry ,pyrrolomycins ,pentabromopseudilin ,Low toxicity ,Bacteria ,business.industry ,Organic Chemistry ,Biological activity ,Bacterial Infections ,antibiofilm agents ,Antimicrobial ,Settore CHIM/08 - Chimica Farmaceutica ,Anti-Bacterial Agents ,Chemistry (miscellaneous) ,Biofilms ,Pentabromopseudilin ,Molecular Medicine ,business ,Antibacterial activity - Abstract
The emergence of antibiotic resistance is currently considered one of the most important global health problem. The continuous onset of multidrug-resistant Gram-positive and Gram-negative bacterial strains limits the clinical efficacy of most of the marketed antibiotics. Therefore, there is an urgent need for new antibiotics. Pyrrolomycins are a class of biologically active compounds that exhibit a broad spectrum of biological activities, including antibacterial, antifungal, anthelmintic, antiproliferative, insecticidal, and acaricidal activities. In this review we focus on the antibacterial activity and antibiofilm activity of pyrrolomycins against Gram-positive and Gram-negative pathogens. Their efficacy, combined in some cases with a low toxicity, confers to these molecules a great potential for the development of new antimicrobial agents to face the antibiotic crisis.
- Published
- 2015
32. New Anti-Adhesion Agents In The Development of Antivirulence Drugs
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CASCIOFERRO, Stella Maria, MAGGIO, Benedetta, RAFFA, Demetrio, RAIMONDI, Maria Valeria, CUSIMANO, Maria Grazia, SCHILLACI, Domenico, MANACHINI, Barbara Rosy Ines, PLESCIA, Fabiana, DAIDONE, Giuseppe, Cascioferro, SM, Maggio, B, Raffa, D, Raimondi, MV, Cusimano, MG, Schillaci, D, Manachini, B, Plescia, F, and Daidone G
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antibiotic resistance ,anti-virulence agents ,Settore BIO/19 - Microbiologia Generale ,Settore CHIM/08 - Chimica Farmaceutica ,biofilm - Abstract
Gram-positive bacteria are a significant cause of nosocomial and community-acquired infections associated with diseases of high morbidity and mortality. Moreover, antibiotic resistance of important Gram-positive pathogens, such as Staphylococcus aureus, Streptococcus pneumoniae and Enterococcus faecalis is one of the major worldwide health problems. Over the last decade, many studies have focused on agents that target the virulence of important pathogens without killing or inhibiting their growth therefore imposing limited selective pressure to promote the development and spread of resistance mechanisms [1]enterococci and streptococci, sortase A plays a critical role in Gram-positive bacterial pathogenesis. It is thus considered a promising target for the development of new anti-infective drugs that aim to interfere with important Gram-positive virulence mechanisms, such as adhesion to host tissues, evasion of host defences, and biofilm formation. The additional properties of sortase A as an enzyme that is not required for Gram-positive bacterial growth or viability and is conveniently located on the cell membrane making it more accessible to inhibitor targeting, constitute additional reasons reinforcing the view that sortase A is an ideal target for anti-virulence drug development. Many inhibitors of sortase A have been identified to date using high-throughput or in silico screening of compound libraries (synthetic or natural. We synthesized two classes of molecules: curcumin analogues and N-phenyl-1H-pyrazole-4-carboxamides able to interfere with the bacterial adhesion, a fundamental step of Gram-positive pathogenesis [2].All derivatives obtained were tested for both the planktonic growth inhibition activity and the inhibition of biofilm formation against the following reference bacterial strains: S. aureus ATCC 6538, S. aureus ATCC 25923 and S. aureus ATCC 29213.
- Published
- 2015
33. Beyond Pain: The Effects of OnabotulinumtoxinA Therapy on Sensitization and Interictal Symptoms in Chronic Migraine.
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Alonge P, Brighina F, Maccora S, Pilati L, Di Marco S, Ventimiglia D, Maggio B, Cutrò I, Camarda C, and Torrente A
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- Humans, Chronic Disease, Migraine Disorders drug therapy, Botulinum Toxins, Type A therapeutic use
- Abstract
Chronic migraine is a disease with a high burden on patients from both a working and quality of life point of view. The pathophysiology of this subtype of migraine is due to several factors, such as medication overuse. Nevertheless, the detrimental recurring of headache attacks with central and peripheral sensitization plays a central role and explains some additional symptoms complained about by these patients even in the interictal phase. OnabotulinumtoxinA is a therapy indicated for chronic migraine since it has proven to reduce peripheral sensitization, showing even efficacy on central symptoms. The aim of this narrative review is to present the current evidence regarding the effect of OnabotulinumtoxinA on sensitization and interictal symptoms.
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- 2024
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34. Frequency and Correlates of Mild Cognitive Impairment in Myasthenia Gravis.
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Iacono S, Di Stefano V, Costa V, Schirò G, Lupica A, Maggio B, Norata D, Pignolo A, Brighina F, and Monastero R
- Abstract
Background: Antibodies against acetylcholine receptors (AChRs) can also target nicotinic AChRs that are present throughout the central nervous system, thus leading to cognitive dysfunctions in patients with myasthenia gravis (MG). However, the presence of cognitive impairment in MG is controversial, and the factors that may influence this risk are almost completely unknown. In this study, the frequency of mild cognitive impairment (MCI) in MG, as well as the clinical, immunological, and behavioral correlates of MCI in MG were evaluated., Methods: A total of 52 patients with MG underwent a comprehensive assessment including motor and functional scales, serological testing, and neuropsychological and behavioral evaluation., Results: The frequency of MCI was 53.8%, and the most impaired cognitive domains were, in order, visuoconstructive/visuospatial skills, memory, and attention. After multivariate analysis, only pyridostigmine use was inversely associated with the presence of MCI, while a trend toward a positive association between MCI and disease severity and arms/legs hyposthenia was found. Correlation analyses showed that daily doses of prednisone and azathioprine significantly correlated with depressive symptomatology, while disease severity significantly correlated with depressive symptomatology and sleep disturbance., Conclusions: The presence of MCI is rather frequent in MG and is characterized by multidomain amnestic impairment. Such preliminary data need further confirmation on larger case series.
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- 2023
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35. Clinical and Neurophysiological Follow-Up of Chronic Inflammatory Demyelinating Polyneuropathy Patients Treated with Subcutaneous Immunoglobulins: A Real-Life Single Center Study.
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Alonge P, Di Stefano V, Lupica A, Gangitano M, Torrente A, Pignolo A, Maggio B, Iacono S, Gentile F, and Brighina F
- Abstract
Background: chronic idiopathic demyelinating polyneuropathy (CIDP) is an acquired, immune-mediated neuropathy characterized by weakness, sensory symptoms and significant reduction or loss of deep tendon reflexes evolving over 2 months at least, associated with electrophysiological evidence of peripheral nerve demyelination. Recently, subcutaneous immunoglobulins (SCIg) have been introduced in clinical practice as a maintenance therapy for CIDP; nevertheless, electrophysiological and efficacy data are limited., Methods: to evaluate SCIg treatment efficacy, we retrospectively reviewed data from 15 CIDP patients referring to our clinic, receiving SCIg treatment and who performed electrophysiological studies (NCS) and clinical scores (MRC sumscore, INCAT disability score and ISS) before starting the treatment and at least one year after., Results: NCS showed no significant changes before and during treatment for all the nerves explored. Clinical scores did not significantly change between evaluations. Correlation analysis evidenced a positive correlation of cMAPs distal amplitude with MRC sumscore and a trend of negative correlation with the INCAT disability score., Conclusions: SCIg maintenance therapy preserves nerve function in CIDP with a good efficacy and safety. Treatment effectiveness can be assessed with ENG, which represents a useful instrument in the follow-up and prognostic assessment of CIDP.
- Published
- 2022
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36. Sound-Induced Flash Illusions Support Cortex Hyperexcitability in Fibromyalgia.
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Di Stefano V, Iacono S, Gagliardo A, Maggio B, Guggino G, Gangitano M, Monastero R, Maggio VR, Bolognini N, and Brighina F
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- Acoustic Stimulation methods, Adult, Anxiety, Auditory Perception physiology, Female, Humans, Infant, Newborn, Middle Aged, Photic Stimulation methods, Fibromyalgia, Illusions physiology, Migraine Disorders
- Abstract
Objectives: Fibromyalgia (FM) is characterized by spontaneous chronic widespread pain in combination with hyperalgesia to pressure stimuli. Sound-induced flash illusions (SIFIs) reflect cross-modal interactions between senses allowing to assess a visual cortical hoerexcitability (VCH) by evaluating the fission and fusion illusions disruption. The aims of the present study were to explore whether SIFIs are perceived differently in patients with fibromyalgia as compared to healthy controls (HCs) and how migraine affects fission and fusion illusions in fibromyalgia., Methods: A single flash (F) accompanied by 0 to 4 beeps (B) was presented to induce the fission illusion while multiple flash (i.e., 2 to 4) accompanied by 0 or 1 beep was presented to induce fusion illusion. The mean number of perceived flashes in fission and fusion illusion trials was compared between the groups (i.e., FM, FM with migraine, and HCs) using repeated-measures analysis of variance. Medication history was recorded along with the administration of Fibromyalgia Impact Questionnaire and Hospital Anxiety and Depression scales., Results: Twenty-four patients with FM (mean age 51, 2 ± 10, 6 years; 22 females), seventeen patients with FM and migraine without aura (mean age 47.8 ± 11.4 years; 16 females; 13 chronic, 4 episodic migraine), and forty-one age- and sex-matched HCs (mean age 47.3 ± 6.9 years; 34 females) participated in the study. Fission and fusion illusory effects were detected in all the participants. However, in FM patients, the fission illusion was reduced and almost abolished as compared to HCs (1F1B, p = 0.02; 1F2B, p < 0.0001; 1F3B, p < 0.0001; 1F4B, p = 0.0001), while there were no differences between groups in fusion trials. Migraine did not affect the fission and the fusion illusions., Conclusion: Results from this study confirm that patients with FM have a VCH suggesting that the pathological changes in cortical excitability might have important roles in the pathophysiology of FM. SIFI represents a noninvasive behavioral tool for the exploration of cross-sensory functional interplay., Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper., (Copyright © 2022 Vincenzo Di Stefano et al.)
- Published
- 2022
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37. Developing a Novel Measurement of Sleep in Rheumatoid Arthritis: Study Proposal for Approach and Considerations.
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Crouthamel M, Mather RJ, Ramachandran S, Bode K, Chatterjee G, Garcia-Gancedo L, Kim J, Alaj R, Wipperman MF, Leyens L, Sillen H, Murphy T, Benecky M, Maggio B, and Switzer T
- Abstract
The development of novel digital endpoints (NDEs) using digital health technologies (DHTs) may provide opportunities to transform drug development. It requires a multidisciplinary, multi-study approach with strategic planning and a regulatory-guided pathway to achieve regulatory and clinical acceptance. Many NDEs have been explored; however, success has been limited. To advance industry use of NDEs to support drug development, we outline a theoretical, methodological study as a use-case proposal to describe the process and considerations when developing and obtaining regulatory acceptance for an NDE to assess sleep in patients with rheumatoid arthritis (RA). RA patients often suffer joint pain, fatigue, and sleep disturbances (SDs). Although many researchers have investigated the mobility of joint functions using wearable technologies, the research of SD in RA has been limited due to the availability of suitable technologies. We proposed measuring the improvement of sleep as the novel endpoint for an anti-TNF therapy and described the meaningfulness of the measure, considerations of tool selection, and the design of clinical validation. The recommendations from the FDA patient-focused drug development guidance, the Clinical Trials Transformation Initiative (CTTI) pathway for developing novel endpoints from DHTs, and the V3 framework developed by the Digital Medicine Society (DiMe) have been incorporated in the proposal. Regulatory strategy and engagement pathways are also discussed., Competing Interests: The authors declare no conflicts of interest. However, all authors are employees and/or stockholders of the companies with which they are affiliated., (Copyright © 2021 by S. Karger AG, Basel.)
- Published
- 2021
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38. 4-(3H)-quinazolinones N-3 substituted with a five membered heterocycle: A promising scaffold towards bioactive molecules.
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Plescia F, Maggio B, Daidone G, and Raffa D
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- Animals, Anti-Infective Agents chemistry, Anti-Infective Agents pharmacology, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents pharmacology, Anticonvulsants chemistry, Anticonvulsants pharmacology, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Antioxidants chemistry, Antioxidants pharmacology, Antitubercular Agents chemistry, Antitubercular Agents pharmacology, Benzothiazoles chemistry, Bronchodilator Agents chemistry, Bronchodilator Agents pharmacology, Humans, Molecular Structure, Nitrogen chemistry, Oxygen chemistry, Quinazolinones pharmacology, Sulfur chemistry, Thiazoles chemistry, Quinazolinones chemistry
- Abstract
The quinazolinone nucleus represents, among the class of fused heterocycles, a very important scaffold to obtain molecules with biological activities. A review of literature revealed how such kind of fused heterocycles, coming from natural or synthetic source, are associated with a wide range of biological activities. This review is mainly directed towards the 4-(3H)-quinazolinones N-3 substituted with a five membered heterocycle in which all the possible combinations of nitrogen, sulfur and oxygen atoms are present., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Masson SAS. All rights reserved.)
- Published
- 2021
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39. Proximal humerus fractures in COVID-19 lockdown: the experience of three orthopedics and traumatology departments in the first ten weeks of the Italian epidemic.
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Maniscalco P, Ciatti C, Gattoni S, Quattrini F, Puma Pagliarello C, Patane' AC, Capelli P, Banchini F, Rivera F, Sanna F, Nonne D, Di Maggio B, and Scaravilli G
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- Adult, Aged, Aged, 80 and over, Female, Humans, Italy epidemiology, Male, Middle Aged, Orthopedics, Traumatology, COVID-19 epidemiology, Humeral Fractures surgery, SARS-CoV-2
- Abstract
Introduction: Coronavirus disease (CoVID-19) is causing millions of deaths worldwide and the crisis of the global healthcare system. Aim Of The Study: evaluate the preliminary impact of CoVID-19 in three Italian Orthopedics and Traumatology Departments in the first 10 weeks of the national lockdown. We focused on proximal humerus fractures, analyzing data and results in comparison with the same period of 2019., Materials and Methods: From February 22nd to May 3rd 2020, 55 patients were admitted to our departments for promixal humerus fractures. Our cohort of patients is composed by 13 males (23.6%) and 42 females (76.4%), with an average age of 73.8 ± 11.7 years (range 44 - 94). Trauma occurred at home in 43 cases (78.2%), by the roadside in 10 cases (18.2%), in a retirement home in 1 case (1.8%), and at work in 1 case (1.8%). We proposed surgical treatment in 15/55 cases, but 4 patients refused hospitalization, mainly because of the risk of contracting n-CoV19 infection., Results: We noticed a decrease in proximal humerus fractures compared to 2019 (-37.5%). Particularly, we observed a significant drop in traumas occurred on the road and at work respectively 23.9% and 3.4%% in 2019, and 18.2% and 1.8% in 2020, probably due to the consequences of the national lockdown. Sports traumas had a reset during the pandemic (6 cases in 2019, 0 in 2020). As consequence, surgical treatment had a decrease due to the reduction in number of fractures, indications and patient's consent., Conclusion: The incidence of proximal humerus fractures had a significant reduction during CoVID-19 spread. We assume that the reasons of this reduction are to be found in the national lockdown (since March 10th, 2020) and Ministerial Decrees that limited the access to the E.R. only in case of severe traumas in order to avoid CoVID-19 spread.
- Published
- 2021
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40. Fractures around Trochanteric Nails: The "Vergilius Classification System".
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Toro G, Moretti A, Ambrosio D, Pezzella R, De Cicco A, Landi G, Tammaro N, Florio P, Cecere AB, Braile A, Medici A, Siano A, Di Maggio B, Calabrò G, Gagliardo N, Di Fino C, Bruno G, Pellegrino A, Negri G, Monaco V, Gison M, Toro A, Schiavone Panni A, Tarantino U, and Iolascon G
- Abstract
Introduction: The fractures that occurred around trochanteric nails (perinail fractures, PNFs) are becoming a huge challenge for the orthopaedic surgeon. Although presenting some specific critical issues (i.e., patients' outcomes and treatment strategies), these fractures are commonly described within peri-implant ones and their treatment was based on periprosthetic fracture recommendations. The knowledge gap about PNFs leads us to convene a research group with the aim to propose a specific classification system to guide the orthopaedic surgeon in the management of these fractures., Materials and Methods: A steering committee, identified by two Italian associations of orthopaedic surgeons, conducted a comprehensive literature review on PNFs to identify the unmet needs about this topic. Subsequently, a panel of experts was involved in a consensus meeting proposing a specific classification system and formulated treatment statements for PNFs. Results and Discussion . The research group considered four PNF main characteristics for the classification proposal: (1) fracture localization, (2) fracture morphology, (3) fracture fragmentation, and (3) healing status of the previous fracture. An alphanumeric code was included to identify each characteristic, allowing to describe up to 54 categories of PNFs, using a 3- to 4-digit code. The proposal of the consensus-based classification reporting the most relevant aspects for PNF treatment might be a useful tool to guide the orthopaedic surgeon in the appropriate management of these fractures., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2021 Giuseppe Toro et al.)
- Published
- 2021
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41. The orthopaedic and traumatology scenario during Covid-19 outbreak in Italy: chronicles of a silent war.
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Benazzo F, Rossi SMP, Maniscalco P, Moretti B, Vaienti E, Ruggieri P, Massè A, Medici A, Formica A, Di Maggio B, Caiaffa V, Mosconi M, Murena L, D'Angelo F, Belluati A, Mazza EL, Rivera F, Castelli A, Ghiara M, Rosolani M, Cioffi R, Pezzella R, Scaravilli G, Bove G, Stissi P, Mazzacane M, Quattrini F, Ciatti C, Trovarelli G, Pala E, Angelini A, Sanna F, Nonne D, Colombelli A, Raggini F, Puzzo A, Canton G, Maritan G, Iuliano A, Randelli P, Solarino G, Moretti L, Vicenti G, Garofalo N, Nappi V, Ripanti S, Chinni C, Pogliacomi F, Visigalli A, Bini N, Aprato A, and Perticarini L
- Subjects
- COVID-19, Disease Outbreaks, Elective Surgical Procedures statistics & numerical data, Emergency Service, Hospital, Humans, Italy epidemiology, Orthopedics, SARS-CoV-2, Trauma Centers, Traumatology, Betacoronavirus, Coronavirus Infections epidemiology, Orthopedic Procedures statistics & numerical data, Pandemics, Pneumonia, Viral epidemiology
- Abstract
Background: From February 21, the day of hospitalisation in ICU of the first diagnosed case of Covid-19, the social situation and the hospitals' organisation throughout Italy dramatically changed., Methods: The CIO (Club Italiano dell'Osteosintesi) is an Italian society devoted to the study of traumatology that counts members spread in public and private hospitals throughout the country. Fifteen members of the CIO, Chairmen of 15 Orthopaedic and Trauma Units of level 1 or 2 trauma centres in Italy, have been involved in the study. They were asked to record data about surgical, outpatients clinics and ER activity from the 23rd of February to the 4th of April 2020. The data collected were compared with the data of the same timeframe of the previous year (2019)., Results: Comparing with last year, overall outpatient activity reduced up to 75%, overall Emergency Room (ER) trauma consultations up to 71%, elective surgical activity reduced up to 100% within two weeks and trauma surgery excluding femoral neck fractures up to 50%. The surgical treatment of femoral neck fractures showed a stable reduction from 15 to 20% without a significant variation during the timeframe., Conclusions: Covid-19 outbreak showed a tremendous impact on all orthopaedic trauma activities throughout the country except for the surgical treatment of femoral neck fractures, which, although reduced, did not change in percentage within the analysed timeframe.
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- 2020
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42. New complex polycyclic compounds: Synthesis, antiproliferative activity and mechanism of action.
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Daidone G, D'Anneo A, Raimondi MV, Raffa D, Hamel E, Plescia F, Lauricella M, and Maggio B
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- Apoptosis drug effects, Autophagy drug effects, Cell Line, Tumor, Crystallography, X-Ray, Humans, Hydrogen Bonding, Polycyclic Compounds chemistry, Structure-Activity Relationship, Cell Proliferation drug effects, Polycyclic Compounds chemical synthesis, Polycyclic Compounds pharmacology
- Abstract
Polycyclic or O-glycoconiugate polycyclic compounds 1a-g were previously tested for their in vitro antiproliferative activity. In this series of compounds, activity increases as log P decreases. Specifically, compounds 1d and 1g showed lower log P values together with the best antiproliferative profiles. With the aim of extending our understanding of the structure-activity relationship (SAR) of this class of compounds, we prepared new polycyclic derivatives 2a-c, which bear on each of the two phenyl rings hydrophilic substituents (OH, SO
2 NH2 or NHCOCH3 ). These substituents are able to form hydrogen bonds and to decrease the partition coefficient value as compared with compound 1d. Compound 2a was slightly more active than 1d, while 2b and 2c had antiproliferative activity comparable to that of 1d. Finally, the role of the two phenyl groups of polycycle derivatives 1 was also investigated. The analog 3, which bears two methyls instead of the two phenyls had a lower log P value (2.94 ± 1.22) than all the other compounds, but it had negligible antiproliferative activity at 10 µM. The analysis of the most active derivative 2a revealed a significant antiproliferative activity against the triple-negative breast cancer cell line MDA-MB231. After a 24 h treatment, an autophagic process was activated, as demonstrated by an increase in monodansylcadaverine-positive cells as well as by the appearance of the autophagic markers Beclin and LC3II. Prolonging the treatment to 48 h, 2a caused cytotoxicity through the activation of caspase-dependent apoptosis., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2020
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43. Novel 4-(3-phenylpropionamido), 4-(2-phenoxyacetamido) and 4-(cinnamamido) substituted benzamides bearing the pyrazole or indazole nucleus: synthesis, biological evaluation and mechanism of action.
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Raffa D, D'Anneo A, Plescia F, Daidone G, Lauricella M, and Maggio B
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- Antineoplastic Agents chemical synthesis, Antineoplastic Agents toxicity, Apoptosis drug effects, Benzamides chemical synthesis, Benzamides toxicity, Cell Line, Tumor, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Humans, Indazoles chemical synthesis, Indazoles toxicity, Proto-Oncogene Proteins c-bcl-2 metabolism, Pyrazoles chemical synthesis, Pyrazoles toxicity, Antineoplastic Agents pharmacology, Benzamides pharmacology, Indazoles pharmacology, Pyrazoles pharmacology
- Abstract
Based on some common structural features of known compounds interfering with p53 pathways and our previously synthesized benzamides, we synthesized new ethyl 5-(4-substituted benzamido)-1-phenyl-1H-pyrazole-4-carboxylates 26a-c, ethyl 5-(4-substituted benzamido)-1-(pyridin-2-yl)-1H-pyrazole-4-carboxylates 27a-c and N-(1H-indazol-6-yl)-4-substituted benzamides 31a,b bearing in the 4 position of the benzamido moiety the 2-phenylpropanamido or 2-phenoxyacetamido or cinnamamido groups. A preliminary test to evaluate the antiproliferative activity against human lung carcinoma H292 cells highlighted how compound 26c showed the best activity. This last was therefore selected for further studies with the aim to find the mechanism of action. Compound 26c induces intrinsic apoptotic pathway by activating p53 and is also able to activate TRAIL-inducing death pathway by promoting increase of DR4 and DR5 death receptors, downregulation of c-FLIP
L and caspase-8 activation., (Copyright © 2018 Elsevier Inc. All rights reserved.)- Published
- 2019
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44. The role of (E)-6-chloro-3-(3-methyl-1-phenyl-1H-pyrazol-5-yl)-2-styrylquinazolin-4(3H)-one in the modulation of cannabinoidergic system. A pilot study.
- Author
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Plescia F, Plescia F, Raffa D, Cavallaro A, Lavanco G, Maggio B, Raimondi MV, Daidone G, Brancato A, and Cannizzaro C
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- Animals, Cannabinoids chemistry, Cyclohexanols chemistry, Dose-Response Relationship, Drug, Locomotion physiology, Pilot Projects, Quinazolines chemistry, Rats, Rats, Wistar, Recognition, Psychology physiology, Styrenes chemistry, Cannabinoids pharmacology, Cyclohexanols pharmacology, Locomotion drug effects, Quinazolines pharmacology, Recognition, Psychology drug effects, Styrenes pharmacology
- Abstract
Background: Compounds acting on endocannabinoid system regulate different neuronal processes through the cannabinoid receptors activation. The main aim of this study was determining whether the 2-styrylquinazolin-4(3H)-one 5, a structural analogue of rimonabant, was able to counteract the behavioural signs of the activation of the endocannabinoidergic system induced by CP 55.940., Methods: Behavioural assessment was carried out using the tetrad task and the novel object recognition test. The endocannabinoidergic system activation was possible by the administration of CP 55.940 and 30min after rats were tested in the tetrad task for the evaluation of the antinociceptive-, cataleptic-, hypothermic- and locomotor- effects. The evaluation of the declarative memory was carried out through the novel object recognition test. The administration of the new compound was made at three different doses, 30min before CP 55.940 administration on a separate group of animals., Results: Our results demonstrated that compound 5, at the highest dose, was able to counteract the effects exerted by CP 55.940, shown by an increase in body temperature, total distance travelled, latency to fall and decrease in tail flick latency, interfering conjointly in memory impairment., Conclusion: This study shows that compound 5 is able to counteract the cannabinoid activation induced by the agonist CP 55.940. Further investigations on its pharmacological profile are mandatory before considering it as a potential candidate for clinical studies and its possible employment as pharmacological agent for the management of different pathological conditions such as motor incoordination, obesity and brain related disorders., (Copyright © 2018 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier B.V. All rights reserved.)
- Published
- 2018
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45. Clearly Detectable, Kinetically Restricted Solid-Solid Phase Transition in cis-Ceramide Monolayers.
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Fanani ML, Busto JV, Sot J, Abad JL, Fabrías G, Saiz L, Vilar JMG, Goñi FM, Maggio B, and Alonso A
- Abstract
Sphingosine [(2 S,3 R,4 E)-2-amino-4-octadecene-1,3-diol] is the most common sphingoid base in mammals. Ceramides are N-acyl sphingosines. Numerous small variations on this canonical structure are known, including the 1-deoxy, the 4,5-dihydro, and many others. However, whenever there is a Δ4 double bond, it adopts the trans (or E) configuration. We synthesized a ceramide containing 4 Z-sphingosine and palmitic acid ( cis-pCer) and studied its behavior in the form of monolayers extended on an air-water interface. cis-pCer acted very differently from the trans isomer in that, upon lateral compression of the monolayer, a solid-solid transition was clearly observed at a mean molecular area ≤44 Å
2 ·molecule-1 , whose characteristics depended on the rate of compression. The solid-solid transition, as well as states of domain coexistence, could be imaged by atomic force microscopy and by Brewster-angle microscopy. Atomistic molecular dynamics simulations provided results compatible with the experimentally observed differences between the cis and trans isomers. The data can help in the exploration of other solid-solid transitions in lipids, both in vitro and in vivo, that have gone up to now undetected because of their less obvious change in surface properties along the transition, as compared to cis-pCer.- Published
- 2018
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46. Can Clinical and Surgical Parameters Be Combined to Predict How Long It Will Take a Tibia Fracture to Heal? A Prospective Multicentre Observational Study: The FRACTING Study.
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Massari L, Benazzo F, Falez F, Cadossi R, Perugia D, Pietrogrande L, Aloj DC, Capone A, D'Arienzo M, Cadossi M, Lorusso V, Caruso G, Ghiara M, Ciolli L, La Cava F, Guidi M, Castoldi F, Marongiu G, La Gattuta A, Dell'Omo D, Scaglione M, Giannini S, Fortina M, Riva A, De Palma PL, Gigante AP, Moretti B, Solarino G, Lijoi F, Giordano G, Londini PG, Castellano D, Sessa G, Costarella L, Barile A, Borrelli M, Rota A, Fontana R, Momoli A, Micaglio A, Bassi G, Cornacchia RS, Castelli C, Giudici M, Monesi M, Branca Vergano L, Maniscalco P, Bulabula M, Zottola V, Caraffa A, Antinolfi P, Catani F, Severino C, Castaman E, Scialabba C, Tovaglia V, Corsi P, Friemel P, Ranellucci M, Caiaffa V, Maraglino G, Rossi R, Pastrone A, Caldora P, Cusumano C, Squarzina PB, Baschieri U, Demattè E, Gherardi S, De Roberto C, Belluati A, Giannini A, Villani C, Persiani P, Demitri S, Di Maggio B, Abate G, De Terlizzi F, and Setti S
- Subjects
- Adolescent, Adult, Female, Humans, Male, Middle Aged, Osteogenesis physiology, Prospective Studies, Reproducibility of Results, Treatment Outcome, Young Adult, Fracture Healing physiology, Tibia physiopathology, Tibia surgery, Tibial Fractures physiopathology, Tibial Fractures surgery
- Abstract
Background: Healing of tibia fractures occurs over a wide time range of months, with a number of risk factors contributing to prolonged healing. In this prospective, multicentre, observational study, we investigated the capability of FRACTING (tibia FRACTure prediction healING days) score, calculated soon after tibia fracture treatment, to predict healing time., Methods: The study included 363 patients. Information on patient health, fracture morphology, and surgical treatment adopted were combined to calculate the FRACTING score. Fractures were considered healed when the patient was able to fully weight-bear without pain., Results: 319 fractures (88%) healed within 12 months from treatment. Forty-four fractures healed after 12 months or underwent a second surgery. FRACTING score positively correlated with days to healing: r = 0.63 ( p < 0.0001). Average score value was 7.3 ± 2.5; ROC analysis showed strong reliability of the score in separating patients healing before versus after 6 months: AUC = 0.823., Conclusions: This study shows that the FRACTING score can be employed both to predict months needed for fracture healing and to identify immediately after treatment patients at risk of prolonged healing. In patients with high score values, new pharmacological and nonpharmacological treatments to enhance osteogenesis could be tested selectively, which may finally result in reduced disability time and health cost savings.
- Published
- 2018
- Full Text
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47. Direct visualization of the lateral structure of giant vesicles composed of pseudo-binary mixtures of sulfatide, asialo-GM1 and GM1 with POPC.
- Author
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Rodi PM, Maggio B, and Bagatolli LA
- Subjects
- 2-Naphthylamine analogs & derivatives, 2-Naphthylamine chemistry, Carbocyanines chemistry, Fluorescent Dyes chemistry, Laurates chemistry, Microscopy, Fluorescence, Molecular Structure, G(M1) Ganglioside chemistry, Lipid Bilayers chemistry, Phosphatidylcholines chemistry, Sulfoglycosphingolipids chemistry, Unilamellar Liposomes chemistry
- Abstract
We compared the lateral structure of giant unilamellar vesicles (GUVs) composed of three pseudo binary mixtures of different glycosphingolipid (GSL), i.e. sulfatide, asialo-GM1 or GM1, with POPC. These sphingolipids possess similar hydrophobic residues but differ in the size and charge of their polar head group. Fluorescence microscopy experiments using LAURDAN and DiIC
18 show coexistence of micron sized domains in a molar fraction range that depends on the nature of the GSLs. In all cases, experiments with LAURDAN show that the membrane lateral structure resembles the coexistence of solid ordered and liquid disordered phases. Notably, the overall extent of hydration measured by LAURDAN between the solid ordered and liquid disordered membrane regions show marked similarities and are independent of the size of the GSL polar head group. In addition, the maximum amount of GSL incorporated in the POPC bilayer exhibits a strong dependence on the size of the GSL polar head group following the order sulfatide>asialo-GM1>GM1. This observation is in full harmony with previous experiments and theoretical predictions for mixtures of these GSL with glycerophospholipids. Finally, compared with previous results reported in GUVs composed of mixtures of POPC with the sphingolipids cerebroside and ceramide, we observed distinctive curvature effects at particular molar fraction regimes in the different mixtures. This suggests a pronounced effect of these GSL on the spontaneous curvature of the bilayer. This observation may be relevant in a biological context, particularly in connection with the highly curved structures found in neural cells., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2018
- Full Text
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48. Recent discoveries of anticancer flavonoids.
- Author
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Raffa D, Maggio B, Raimondi MV, Plescia F, and Daidone G
- Subjects
- Animals, Benzopyrans chemistry, Benzopyrans pharmacology, Humans, Plants chemistry, Structure-Activity Relationship, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic pharmacology, Drug Discovery methods, Flavonoids chemistry, Flavonoids pharmacology, Neoplasms drug therapy
- Abstract
In this review we report the recent advances in anticancer activity of the family of natural occurring flavonoids, covering the time span of the last five years. The bibliographic data will be grouped, on the basis of biological information, in two great categories: reports in which the extract plants bioactivity is reported and the identification of each flavonoid is present or not, and reports in which the anticancer activity is attributable to purified and identified flavonoids from plants. Wherever possible, the targets and mechanisms of action as well as the structure-activity relationships of the molecules will be reported. Also, in the review it was thoroughly investigated the recent discovery on flavonoids containing the 2-phenyl-4H-chromen-4-one system even if some examples of unusual flavonoids, bearing a non-aromatic B-ring or other ring condensed to the base structure are reported., (Copyright © 2017 Elsevier Masson SAS. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
49. PEEK radiolucent plate for distal radius fractures: multicentre clinical results at 12 months follow up.
- Author
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Di Maggio B, Sessa P, Mantelli P, Maniscalco P, Rivera F, Calori GM, Bisogno L, Scaravilli G, and Caforio M
- Subjects
- Adult, Aged, Aged, 80 and over, Benzophenones, Bone Screws, Carbon, Carbon Fiber, Female, Follow-Up Studies, Humans, Ketones, Male, Middle Aged, Polyethylene Glycols, Polymers, Radius Fractures diagnostic imaging, Radius Fractures physiopathology, Range of Motion, Articular physiology, Retrospective Studies, Treatment Outcome, Wrist Joint physiopathology, Young Adult, Bone Plates, Fracture Fixation, Internal instrumentation, Fracture Fixation, Internal methods, Fracture Healing physiology, Radiography, Radius Fractures surgery, Wrist Joint diagnostic imaging
- Abstract
Introduction: Open reduction and internal fixation (ORIF) with plate and screws represents the recommended treatment for unstable intra-articular distal radius fractures. Although significant progresses in surgical technique have been made, anatomical reconstruction of radio-carpal articular surface still represent a difficult task, especially in multifragmentary fractures. Available PEEK reinforced-carbon fiber composite radiolucent devices allow both an easier and more careful assessment of intra-operative reduction of the articular surface of distal radius and prompt correction of any residual step deformity., Materials and Methods: We retrospectively reviewed clinical and radiological multicentre results of 71 consecutive AO B and C fracture pattern of distal radius treated using the same PEEK reinforced-carbon fiber composite radiolucent plate., Results: Three patients lost at final follow up and 4 cases with incomplete radiological documentations were excluded from the study. 64 patients (38 females, 26 males) were available and formed the basis of this report. Fracture types included 9 patients with 23-B, 13 patients 23-B2,15 patients with 23-B3,10 patients with 23-C1, 7 patients with 23-C2 and 10 patients with 23-C3. Mean Modified Mayo wrist Score was on average 38.11 (SD 10.1; range 24-75, 95%CI 34.7-41.4), 67.22 (SD 9.6, range 50-90, 95%CI 64-70.4), 90.54 (SD 6.3, range 75-100, 95%CI 88.4-92.6) at one, two and twelve months of follow-up, respectively. A statistically significant difference was found between mean scores at different follow-up periods (p = 0.001). We noted 1 case of distal screw fixation aseptic loosening at 5 months post surgical intervention., Conclusions: PEEK reinforced-carbon fiber composite radiolucent plate represents a useful device for treatment of complex distal radius fractures in the adult population. It possesses unique biomechanical properties and allows for an easier anatomical reduction during surgical intervention., (© 2017 Elsevier Ltd. All rights reserved.)
- Published
- 2017
- Full Text
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50. The many faces (and phases) of ceramide and sphingomyelin I - single lipids.
- Author
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Fanani ML and Maggio B
- Abstract
Ceramides, the simplest kind of two-chained sphingolipids, contain a single hydroxyl group in position 1 of the sphingoid base. Sphingomyelins further contain a phosphocholine group at the OH of position 1 of ceramide. Ceramides and sphingomyelins show a variety of species depending on the fatty acyl chain length, hydroxylation, and unsaturation. Because of the relatively high transition temperature of sphingomyelin compared to lecithin and, particularly, of ceramides with 16:0-18:0 saturated chains, a widespread idea on their functional importance refers to formation of rather solid domains enriched in sphingomyelin and ceramide. Frequently, and especially in the cell biology field, these are generally (and erroneously) assumed to occur irrespective on the type of N-acyl chain in these lipids. This is because most studies indicating such condensed ordered domains employed sphingolipids with acyl chains with 16 carbons while scarce attention has been focused on the influence of the N-acyl chain on their surface properties. However, abundant evidence has shown that variations of the N-acyl chain length in ceramides and sphingomyelins markedly affect their phase state, interfacial elasticity, surface topography, electrostatics and miscibility and that, even the usually conceived "condensed" sphingolipids and many of their mixtures, may exhibit liquid-like expanded states. This review is a summarized overview of our work and of related others on some facts regarding membranes composed of single molecular species of ceramide and sphingomyelin. A second part is dedicated to discuss the miscibility properties between species of sphingolipids that differ in N-acyl and oligosaccharide chains.
- Published
- 2017
- Full Text
- View/download PDF
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