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7 results on '"Macrae, Sheila"'

3. Additional file 7: Figure S1. of Comprehensive transcriptional landscape of aging mouse liver

Author

White, Ryan, Milholland, Brandon, MacRae, Sheila, Mingyan Lin, Deyou Zheng, and Vijg, Jan

Abstract

RNAseq validation. (a) qPCR validation of 13 selected transcripts normalized to eukaryotic 18 s rRNA using TaqMan primer/probe sets. (b) qPCR validation of 13 differentially expressed non-coding RNAs normalized to GAPDH mRNA using custom made primer for each novel ncRNA. For both, RNA-seq and qPCR values are plotted as the mean (Old, n = 3; Young, n = 3) log2FoldChange(Old/Young) value, where the red line indicates the slope of linear regression and the square of Pearson’s correlation coefficient (r). Insets are bar plots of equivalent corresponding values. (PDF 122 kb)

Published
2015
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4. Additional file 8: Figure S2. of Comprehensive transcriptional landscape of aging mouse liver

Author

White, Ryan, Milholland, Brandon, MacRae, Sheila, Mingyan Lin, Deyou Zheng, and Vijg, Jan

Abstract

Representative subset (Meg3, Rian and Mirg) of maternally expressed and imprinted ncRNA locus, Dlk-Dio3, on chromosome 12 in UCSC Genome Browser overlaid with aligned reads from RNA-seq dataset. Blue = sense paired-end, red = antisense paired-end; Scale = 100 kb. (PDF 64 kb)

Published
2015
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5. Comparative analysis of genome maintenance genes in naked mole rat, mouse, and human

Author

MacRae, Sheila, Zhang, Quanwei, Lemetre, Christophe, Seim, Inge, Calder, Robert, Hoeijmakers, Jan, Suh, Yousin, Gladyshev, Vadim, Seluanov, Andrei, Gorbunova, Vera, Vijg, Jan, Zhang, Zhengdong, MacRae, Sheila, Zhang, Quanwei, Lemetre, Christophe, Seim, Inge, Calder, Robert, Hoeijmakers, Jan, Suh, Yousin, Gladyshev, Vadim, Seluanov, Andrei, Gorbunova, Vera, Vijg, Jan, and Zhang, Zhengdong

Abstract

Genome maintenance (GM) is an essential defense system against aging and cancer, as both are characterized by increased genome instability. Here, we compared the copy number variation and mutation rate of 518 GM-associated genes in the naked mole rat (NMR), mouse, and human genomes. GM genes appeared to be strongly conserved, with copy number variation in only four genes. Interestingly, we found NMR to have a higher copy number of CEBPG, a regulator of DNA repair, and TINF2, a protector of telomere integrity. NMR, as well as human, was also found to have a lower rate of germline nucleotide substitution than the mouse. Together, the data suggest that the long-lived NMR, as well as human, has more robust GM than mouse and identifies new targets for the analysis of the exceptional longevity of the NMR.

Published
2015

6. DNA repair in species with extreme lifespan differences

Author

MacRae, Sheila L, primary, Croken, Matthew McKnight, additional, Calder, R.B, additional, Aliper, Alexander, additional, Milholland, Brandon, additional, White, Ryan R, additional, Zhavoronkov, Alexander, additional, Gladyshev, Vadim N, additional, Seluanov, Andrei, additional, Gorbunova, Vera, additional, Zhang, Zhengdong D, additional, and Vijg, Jan, additional

Published
2015
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