19 results on '"Ma KN"'
Search Results
2. Resurrection of Perilimnastes (Sonerileae, Melastomataceae) with description of a new species P.nana .
- Author
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Liu Y, Dai JH, Zhuang QY, Zou CY, and Ma KN
- Abstract
Recent research has indicated that the Phyllagathis (raphides) clade (Sonerileae, Melastomataceae) is only distantly related to the type of Phyllagathis and should be separated as a distinct genus. Phylogeny of this clade is here reconstructed with expanded taxon sampling. Four strongly supported subclades have been identified. The possible affinities of taxa that were not sampled in the analysis are discussed, based on morphological data. Perilimnastes is resurrected as the generic name of the Phyllagathis (raphides) clade. A generic description, colour figures, map of distribution, a list of included species and a key are provided for Perilimnastes . Fifteen new combinations are made plus the description of a new species. As interpreted here, Perilimnastes consists of twenty species and two varieties., Competing Interests: The authors have declared that no competing interests exist., (Ying Liu, Jin-Hong Dai, Qi-Yuan Zhuang, Chun-Yu Zou, Kai-Nan Ma.)
- Published
- 2024
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3. Trichinella spiralis galectin binding to toll-like receptor 4 induces intestinal inflammation and mediates larval invasion of gut mucosa.
- Author
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Ma KN, Zhang Y, Zhang ZY, Wang BN, Song YY, Han LL, Zhang XZ, Long SR, Cui J, and Wang ZQ
- Subjects
- Mice, Animals, Humans, Toll-Like Receptor 4 genetics, NF-kappa B metabolism, Caco-2 Cells, Larva physiology, Galectins, Interleukin-6, Intestinal Mucosa metabolism, Cytokines metabolism, Inflammation veterinary, Transforming Growth Factor beta, Trichinella spiralis physiology
- Abstract
Previous studies showed that Trichinella spiralis galectin (Tsgal) facilitates larval invasion of intestinal epithelium cells (IECs). However, IEC proteins binding with Tsgal were not identified, and the mechanism by which Tsgal promotes larval invasion is not clear. Toll-like receptors (TLRs) are protein receptors responsible for recognition of pathogens. The aim of this study was to investigate whether recombinant Tsgal (rTsgal) binds to TLR-4, activates inflammatory pathway in gut epithelium and mediates T. spiralis invasion. Indirect immunofluorescence (IIF), GST pull-down and co-immunoprecipitation (Co-IP) assays confirmed specific binding between rTsgal and TLR-4 in Caco-2 cells. qPCR and Western blotting showed that binding of rTsgal with TLR-4 up-regulated the TLR-4 transcription and expression in Caco-2 cells, and activated p-NF-κB p65 and p-ERK1/2. Activation of inflammatory pathway TLR-4/MAPK-NF-κB by rTsgal up-regulated pro-inflammatory cytokines (IL-1β and IL-6) and down-regulated anti-inflammatory cytokine TGF-β in Caco-2 cells, and induced intestinal inflammation. TAK-242 (TLR-4 inhibitor) and PDTC (NF-κB inhibitor) significantly inhibited the activation of TLR-4 and MAPK-NF-κB pathway. Moreover, the two inhibitors also inhibited IL-1β and IL-6 expression, and increased TGF-β expression in Caco-2 cells. In T. spiralis infected mice, the two inhibitors also inhibited the activation of TLR-4/MAPK-NF-κB pathway, ameliorated intestinal inflammation, impeded larval invasion of gut mucosa and reduced intestinal adult burdens. The results showed that rTsgal binding to TLR-4 in gut epithelium activated MAPK-NF-κB signaling pathway, induced the expression of TLR-4 and pro-inflammatory cytokines, and mediated larval invasion. Tsgal might be regarded as a candidate molecular target of vaccine against T. spiralis enteral invasive stage., (© 2023. The Author(s).)
- Published
- 2023
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4. A novel C-type lectin from Trichinella spiralis mediates larval invasion of host intestinal epithelial cells.
- Author
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Hao HN, Song YY, Ma KN, Wang BN, Long SR, Liu RD, Zhang X, Wang ZQ, and Cui J
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- Mice, Animals, Larva genetics, DNA, Complementary, Lectins, C-Type metabolism, Mannose metabolism, Helminth Proteins metabolism, Mice, Inbred BALB C, Epithelial Cells metabolism, Trichinella spiralis, Trichinellosis parasitology, Trichinellosis veterinary, Vaccines
- Abstract
The aim of this study was to investigate the characteristics of a novel type C lectin from Trichinella spiralis (TsCTL) and its role in larval invasion of intestinal epithelial cells (IECs). TsCTL has a carbohydrate recognition domain (CRD) of C-type lectin. The full-length TsCTL cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of qPCR, Western blotting and immunofluorescence assays (IFAs) showed that TsCTL was a surface and secretory protein that was highly expressed at the T. spiralis intestinal infective larva (IIL) stages and primarily located at the cuticle, stichosome and embryos of the parasite. rTsCTL could specifically bind with IECs, and the binding site was localized in the IEC nucleus and cytoplasm. The IFA results showed that natural TsCTL was secreted and bound to the enteral epithelium at the intestinal stage of T. spiralis infection. The rTsCTL had a haemagglutinating effect on murine erythrocytes, while mannose was able to inhibit the rTsCTL agglutinating effect for mouse erythrocytes. rTsCTL accelerated larval intrusion into the IECs, whereas anti-rTsCTL antibodies and mannose significantly impeded larval intrusion in a dose-dependent manner. The results indicated that TsCTL specifically binds to IECs and promotes larval invasion of intestinal epithelium, and it might be a potential target of vaccines against T. spiralis enteral stages., (© 2022. The Author(s).)
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- 2022
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5. [Finite element analyses of retention of removable partial denture circumferential clasps manufactured by selective laser melting].
- Author
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Ma KN, Chen H, Shen YR, Zhou YS, Wang Y, and Sun YC
- Subjects
- Chromium Alloys, Dental Clasps, Denture Retention, Finite Element Analysis, Humans, Lasers, Titanium, Denture, Partial, Removable
- Abstract
Objective: To compare the retentions of different designs of cobalt-chromium (Co-Cr), pure titanium (CP Ti), and titanium alloy (Ti-6Al-4V) removable partial denture (RPD) circumferential clasps manufactured by selective laser melting (SLM) and to analyze the stress distribution of these clasps during the removal from abutment teeth., Methods: Clasps with clasp arm size A (1.9 mm width/1.1 mm thickness at the body and 0.8-taper) or B (1.2 times A) and 0.25 mm or 0.50 mm undercut engagement were modeled on a prepared first premolar die, named as designs A1, A2, A3, and A4, respectively. The density and elastic modulus of SLM-built Co-Cr, CP Ti, and Ti-6Al-4V were measured and given to different groups of clasps. The density, elastic modulus, and Poisson ' s ratio of enamel were given to the die. The control group was the cast Co-Cr clasp with design A1, to which the density and elastic modulus of cast Co-Cr alloy were given. The Poisson's ratio of all metals was 0.33. The initial 5 N dislodging force was applied, and the maximum displacement of the clasp along the insertion path was computed. The load was reapplied with an increment of 5 N than in the last simulation until the clasp was completely dislodged. The retentive force range of different groups of clasps was obtained. The retentive forces of the SLM-built Co-Cr, CP Ti, and Ti-6Al-4V clasps with equivalent computed retentive force range to the control group were validated through the insertion/removal experiment. The von Mises stress distributions of these three groups of SLM-built clasps under 15 N loads were analyzed., Results: SLM-built Co-Cr, CP Ti, and Ti-6Al-4V clasps with designs B1 or B2, and Co-Cr clasps with design A2 had higher retentive forces than those of the control group. SLM-built CP Ti and Ti-6Al-4V clasps with design A1 had lower retentive forces than those of the control group. SLM-built Co-Cr clasp with design A1 and SLM-built CP Ti and Ti-6Al-4V clasps with design A2 had equivalent retentive forces to those of the control group. The insertion/removal experiment showed that the measured retentive forces of these three groups of SLM-built clasps were (21.57±5.41) N, (19.75±4.47) N, and (19.32±2.04) N, respectively. No statistically significant measured retentive force difference was found among these three groups of SLM-built clasps ( P >0.05). The maximum von Mises stress of these three groups of SLM-built clasps exceeded their responding yield strength except for the Ti-6Al-4V one., Conclusion: SLM-built Co-Cr circumferential clasps had higher retention than CP Ti and Ti-6Al-4V ones with the same clasp arm size and undercut engagement. The retention of SLM-built circumferential clasps could be adjusted by changing the undercut engagement and clasp arm size. If SLM-built circumferential clasps are used in clinical practice, the Ti-6Al-4V clasp with clasp arm size A and 0.50 mm undercut engagement is recommended considering the long-term use of RPD in the patient's mouth.
- Published
- 2022
6. Molecular characterization of a novel cathepsin L from Trichinella spiralis and its participation in invasion, development and reproduction.
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Bai Y, Ma KN, Sun XY, Dan Liu R, Long SR, Jiang P, Wang ZQ, and Cui J
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- Animals, Cathepsin L, Female, Helminth Proteins, Larva genetics, Mice, Mice, Inbred BALB C, Reproduction, Trichinella spiralis genetics, Trichinellosis
- Abstract
Cathepsin L is one member of cysteine protease superfamily and widely distributed in parasitic organisms, it plays the important roles in worm invasion, migration, nutrient intake, molting and immune evasion. The objective of this study was to investigate the biological characteristics of a novel cathepsin L from Trichinella spiralis (TsCL) and its role in larval invasion, development and reproduction. TsCL has a functional domain of C1 peptidase, which belongs to cathepsin L family. The complete TsCL sequence was cloned and expressed in Escherichia coli BL21. The rTsCL has good immunogenicity. RT-PCR and Western blotting analysis showed that TsCL was transcribed and expressed at different T. spiralis phases (e.g., muscle larvae, intestinal infectious larvae, adult worms and newborn larvae). Immunofluorescence test revealed that TsCL was principally localized in the cuticle, stichosome, midgut and female intrauterine embryos of the nematode. rTsCL has the capacity to specially bind with intestinal epithelial cells (IECs) and the binding sites was located in the cytoplasm. rTsCL promoted larval penetration into IEC, while anti-rTsCL antibodies inhibited the invasion. The silencing of TsCL gene by specific dsRNA significantly reduced the TsCL expression and enzyme activity, and also reduced larval invasive ability, development and female reproduction. The results showed that TsCL is an obligatory protease in T. spiralis lifecycle. TsCL participates in worm invasion, development and reproduction, and may be regarded as a potential candidate vaccine/drug target against T. spiralis infection., (Copyright © 2021. Published by Elsevier B.V.)
- Published
- 2021
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7. Molecular cloning and characterization of a novel aspartyl aminopeptidase from Trichinella spiralis.
- Author
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Sun XY, Ma KN, Bai Y, Liu RD, Long SR, Zhang X, Jiang P, Ciu J, and Wang ZQ
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- Animals, Cloning, Molecular, Erythrocytes parasitology, Female, Mice, Mice, Inbred BALB C, Trichinella spiralis genetics, Trichinellosis, Glutamyl Aminopeptidase genetics, Helminth Proteins genetics, Trichinella spiralis enzymology
- Abstract
Trichinellosis is an important zoonotic parasitic disease worldwide and is principally caused by ingesting animal meat containing Trichinella infective larvae. Aspartyl aminopeptidase is an intracytoplasmic metalloproteinase that specifically hydrolyzes the N-terminus of polypeptides free of acidic amino acids (aspartic acid and glutamate), and plays an important role in the metabolism, growth and development of organisms. In this study, a novel T. spiralis aspartyl aminopeptidase (TsAAP) was cloned and expressed, and its biological properties and roles in worm growth and development were investigated. The results revealed that TsAAP transcription and expression in diverse T. spiralis stages were detected by RT-PCR and Western blotting, and primarily localized at cuticle, stichosome and intrauterine embryos of this nematode by immunofluorescence test. rTsAAP has the enzymatic activity of native AAP to hydrolyze the substrate H-Glu-pNA. There was a specific binding between rTsAAP and murine erythrocyte, and the binding site was localized in erythrocyte membrane proteins. Silencing of TsAAP gene by specific dsRNA significantly reduced the TsAAP expression, enzymatic activity, intestinal worm burdens and female fecundity. The results demonstrated that TsAAP participates in the growth, development and fecundity of T. spiralis and it might be a potential target molecule for anti-Trichinella vaccines.
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- 2021
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8. [Quantitative evaluation of printing accuracy of multi-color and multi-hardness three-dimensional printing dental model with photopolymer jetting].
- Author
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Shen YR, Chen H, Ma KN, and Sun YC
- Subjects
- Hardness, Maxilla diagnostic imaging, Printing, Three-Dimensional, Computer-Aided Design, Models, Dental
- Abstract
Objective: To quantitatively evaluate the accuracy of multi-color and multi-hardness dental models printed by using the photopolymer jetting (PJ) technology, and to provide protocol for the clinical application. Methods: A maxillary partially edentulous (Kennedy class Ⅱ subclass 1) standard digital model obtained through scanning and processing was selected as reference data. Five monochromatic DLP (digital light processing) models with single hardness were printed by printer DLP-800d based on DLP technology (DLP group), and five multi-color and multi-hardness PJ models were printed by printer J300Plus based on PJ technology (PJ group). Scan the printed model and register the scanning data to the reference data in Geomagic Studio 2013 software. The three-dimensional (3D) deviations of the whole and each area, including residual dentition, abutments adjacent to the edentulous area, gingiva, gingiva in the distal-extended edentulous area, gingiva in other edentulous areas, gingiva supporting the removable partial denture (RPD), were calculated and represented by the root mean square error (RMS) value. The smaller the RMS value was, the higher the trueness of printing was. The scanning data of the five models in the same group were registered in pairs to calculate the 3D deviation. The smaller the RMS value was, the higher the precision of printing was. The threshold of clinical acceptability was 200 μm. Statistical analysis was performed to compare the difference of trueness and precision between the two groups. Results: The overall trueness of the DLP group [57.70 (2.10) μm] was significantly better than that of the PJ group [71.00 (7.70) μm]. The overall precision of the DLP group [15.20 (5.05) μm] was significantly better than that of the PJ group [37.55 (15.55) μm]. The overall trueness and precision of both groups were within the clinically acceptable range. Conclusions: The domestic PJ printer used in this study can print multi-color and multi-hardness dental models with good trueness and precision, which can provide integrated 3D printing technology support for realizing the simulation of regional hardness differentiation between soft and hard tissues of dental models.
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- 2021
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9. [Advances in computer aided design and computer aided manufacturing of removable partial denture].
- Author
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Ma KN, Chen H, Ye HQ, Zhou YS, Wang Y, and Sun YC
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- Computer-Aided Design, Lasers, Tooth, Artificial, Workflow, Denture, Partial, Removable
- Abstract
Computer aided design, numerically controlled milling, additive manufacturing and other digital technologies have been widely used in clinical practice of prosthodontics. The application of these technologies not only changed the design and manufacturing workflow of removable partial denture (RPD), but also improved the production efficiency of dentures, and changed the design and manufacturing concept of dentures. A large number of researches on computer aided design and computer aided manufacturing (CAD/CAM) of RPD have emerged in recent years, including researches on the properties of selective laser melting metal powder and other new dental materials, the innovative design of retainers, connectors, artificial teeth and other denture components, high precision manufacturing of the RPD framework, denture base and artificial teeth, quantitative laboratory evaluations of the accuracy, adaption and mechanical properties of RPD with new structures and made of new materials, clinical evaluations of RPD and others. This paper introduces these recent developments in CAD/CAM of RPD.
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- 2021
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10. Identification of Biomarkers Related to Neuropathic Pain Induced by Peripheral Nerve Injury.
- Author
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Zhang CG, Wan HQ, Ma KN, Luan SX, and Li H
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- Animals, Biomarkers metabolism, Gene Regulatory Networks, Neuralgia etiology, Neuralgia metabolism, Peripheral Nerve Injuries complications, Peripheral Nerve Injuries metabolism, Rats, Sciatic Nerve injuries, Neuralgia genetics, Peripheral Nerve Injuries genetics, Protein Interaction Maps
- Abstract
Our study aimed to explore the molecular mechanisms and novel target genes of neuropathic pain via bioinformatics analysis. Gene expression profiling of GSE30691 which was consisted of sciatic nerve lesion and sham control samples at 3 days, 7 days, 21 days, and 40 days (D3, D7, D21, and D40) after injury were downloaded from Gene Expression Omnibus. Differentially expressed genes (DEGs) were identified for all the four time points. Overlapped DEGs for all the four time points were used for functional and weighted co-expression modular analysis. Afterwards, protein-protein interaction (PPI) network was analyzed by MCODE (Molecular Complex Detection) and BiNGO. Pathway network was constructed according to the enriched pathways of PPI network and relevant pathways selected from the Comparative Toxicogenomics Database. There were 355 overlapped DEGs for all the four time points. Two co-expression modules had significant positive correlations with disease. The top ten hub DEGs in the PPI network were Fos, Tp53, Csk, Map2k2, Stat3, Ccl2, Pxn, Tgfb1, Notch1, and Prkacb. Fos, Dusp1, Tp53, Tgfb1, and Map2k2 participated in MAPK signaling pathway, while Csk participated in chemokine signaling pathway. The expressions of Fos, Tp53, Csk, and Map2k2 were significantly increased at D3. Tp53, Csk, and Map2k2 continued overexpressing until at D7, and an elevated tendency in Csk expression could be observed until at D21. The expression of Fos reached up to the highest at D40. Fos, Tp53, Csk, and Map2k2 might be the potential biomarkers related to neuropathic pain.
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- 2019
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11. Peripapillary Retinoschisis in Glaucoma: Association With Progression and OCT Signs of Müller Cell Involvement.
- Author
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Fortune B, Ma KN, Gardiner SK, Demirel S, and Mansberger SL
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- Aged, Aged, 80 and over, Case-Control Studies, Disease Progression, Female, Glaucoma, Open-Angle diagnostic imaging, Humans, Intraocular Pressure, Male, Middle Aged, Ocular Hypertension diagnostic imaging, Ocular Hypertension physiopathology, Retinoschisis diagnostic imaging, Tomography, Optical Coherence methods, Visual Fields physiology, Ependymoglial Cells pathology, Glaucoma, Open-Angle physiopathology, Nerve Fibers pathology, Optic Disk pathology, Retinal Ganglion Cells pathology, Retinoschisis physiopathology
- Abstract
Purpose: To examine demographic and clinical factors associated with glaucomatous peripapillary retinoschisis (PPRS) and assess its association with glaucoma progression., Methods: Using a case control study design and longitudinal data from a cohort of 166 subjects with a diagnosis of glaucoma or glaucoma suspect, we compared functional, structural, clinical, and demographic characteristics between PPRS cases and controls., Results: The frequency of PPRS was 6.0% (12 eyes from 10/166 subjects) with two eyes having PPRS in different sectors for a total of 15 retinoschisis events. There were no significant differences (P > 0.05) in age, sex, visual acuity, central corneal thickness, intraocular pressure, or presence of vitreous adhesion between PPRS and controls. However, eyes with PPRS tended to have a higher cup-to-disc ratio (P = 0.06), thinner RNFL (P = 0.02), and worse visual field mean deviation (MD, P = 0.06) than controls. The rate of RNFL thinning was faster in PPRS (average: -2.8%/year; range: -7.4% to 0.0%/year) than controls (-1.3%/year; range: -4.4% to 0.6%/year; P = 0.021). The rate of visual field MD change was faster in PPRS (-0.49 dB/year; range: -2.0 to 0.9 dB/year) than controls (-0.06 dB/year; range: -0.8 to 0.3 dB/year; P = 0.030). OCT scans showed hyperreflective structures spanning the PPRS whose morphology and spacing (50 ± 7 μm) are consistent with Müller glia, causing signal attenuation casting "shadows" onto distal retina., Conclusions: This is the first report showing that glaucomatous PPRS is associated with a faster overall rate of RNFL thinning and visual field deterioration and to specifically identify OCT signs of Müller cell involvement.
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- 2018
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12. Projection-Resolved Optical Coherence Tomography Angiography of Macular Retinal Circulation in Glaucoma.
- Author
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Takusagawa HL, Liu L, Ma KN, Jia Y, Gao SS, Zhang M, Edmunds B, Parikh M, Tehrani S, Morrison JC, and Huang D
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- Aged, Algorithms, Female, Fluorescein Angiography, Glaucoma, Open-Angle diagnosis, Humans, Intraocular Pressure physiology, Low Tension Glaucoma diagnosis, Male, Middle Aged, Nerve Fibers pathology, Prospective Studies, ROC Curve, Retinal Ganglion Cells pathology, Visual Field Tests, Visual Fields physiology, Blood Circulation physiology, Computed Tomography Angiography, Glaucoma, Open-Angle physiopathology, Low Tension Glaucoma physiopathology, Retinal Vessels physiology, Tomography, Optical Coherence methods
- Abstract
Purpose: To detect macular perfusion defects in glaucoma using projection-resolved optical coherence tomography (OCT) angiography., Design: Prospective observation study., Participants: A total of 30 perimetric glaucoma and 30 age-matched normal participants were included., Methods: One eye of each participant was imaged using 6×6-mm macular OCT angiography (OCTA) scan pattern by 70-kHz 840-nm spectral-domain OCT. Flow signal was calculated by the split-spectrum amplitude-decorrelation angiography algorithm. A projection-resolved OCTA (PR-OCTA) algorithm was used to remove flow projection artifacts. Four en face OCTA slabs were analyzed: the superficial vascular complex (SVC), intermediate capillary plexus (ICP), deep capillary plexus (DCP), and all-plexus retina (SVC + ICP + DCP). The vessel density (VD), defined as the percentage area occupied by flow pixels, was calculated from en face OCTA. A novel algorithm was used to adjust the vessel density to compensate for local variations in OCT signal strength., Main Outcome Measures: Macular retinal VD, ganglion cell complex (GCC) thickness, and visual field (VF) sensitivity., Results: Focal capillary dropout could be visualized in the SVC, but not the ICP and DVP, in glaucomatous eyes. In the glaucoma group, the SVC and all-plexus retinal VD (mean ± standard deviation: 47.2%±7.1% and 73.5%±6.6%) were lower than in the normal group (60.5%±4.0% and 83.2%±4.2%, both P < 0.001, t test). The ICP and DCP VD were not significantly lower in the glaucoma group. Among the overall macular VD parameters, the SVC VD had the best diagnostic accuracy as measured by the area under the receiver operating characteristic curve (AROC). The accuracy was even better when the worse hemisphere (inferior or superior) was used, achieving an AROC of 0.983 and a sensitivity of 96.7% at a specificity of 95%. Among the glaucoma participants, the hemispheric SVC VD values were highly correlated with the corresponding GCC thickness and VF sensitivity (P < 0.003). The reflectance compensation step in VD calculation significantly improved repeatability, normal population variation, and correlation with VF and GCC thickness., Conclusions: On the basis of PR-OCTA, glaucoma preferentially affects perfusion in the SVC in the macula more than the deeper plexuses. Reflectance-compensated SVC VD measurement by PR-OCTA detected glaucoma with high accuracy and could be useful in the clinical evaluation of glaucoma., (Copyright © 2017 American Academy of Ophthalmology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
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13. A New Variant of Polypoidal Choroidal Vasculopathy With Annular Pigmentary Changes in Haitian Males.
- Author
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Thanos A, Miller JB, Ma KN, Subramanian ML, Kim IK, and Eliott D
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- Adult, Aged, Angiogenesis Inhibitors therapeutic use, Choroidal Neovascularization therapy, Combined Modality Therapy, Exudates and Transudates, Fluorescein Angiography, Haiti, Humans, Male, Middle Aged, Multimodal Imaging, Photochemotherapy, Polyps drug therapy, Retinal Detachment therapy, Retinal Hemorrhage therapy, Tomography, Optical Coherence, Vascular Endothelial Growth Factor A antagonists & inhibitors, Choroidal Neovascularization diagnosis, Polyps diagnosis, Retinal Detachment diagnosis, Retinal Hemorrhage diagnosis, Retinal Pigment Epithelium pathology
- Abstract
The authors report a new variant of idiopathic polypoidal choroidal vasculopathy (IPCV) in middle-aged Haitian men characterized by extramacular polypoidal lesions and bilateral extensive pigmentary alterations in the posterior pole in an annular wreath-like pattern surrounding the optic nerve and macular area. Two patients were seen at Massachusetts Eye and Ear Infirmary and one at Boston University Medical Center between 2010 and 2015. All three patients were middle-aged Haitian men who exhibited bilateral features of IPCV, including subretinal hemorrhages and serosanguinous pigment epithelial detachments. Indocyanine green angiography revealed extramacular polypoidal lesions located mostly along the major vascular arcades. Extensive pigmentary alterations were evident in the posterior pole surrounding the macula and optic nerve in an annular wreath-like pattern. These cases further expand the clinical spectrum of IPCV., (Copyright 2016, SLACK Incorporated.)
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- 2016
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14. A Novel Technique for Amniotic Membrane Transplantation in Patients with Acute Stevens-Johnson Syndrome.
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Ma KN, Thanos A, Chodosh J, Shah AS, and Mantagos IS
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- Acute Disease, Humans, Amnion transplantation, Ophthalmologic Surgical Procedures methods, Stevens-Johnson Syndrome surgery
- Abstract
Cryopreserved amniotic membrane (AM) transplantation is an emerging technique that is becoming the gold standard for the management of acute Stevens-Johnson syndrome (SJS) and its more severe variant, toxic epidermal necrolysis (TEN). We describe a novel surgical technique utilizing a single, large sheet of AM (5 x 10 cm) and a custom-made forniceal ring, which facilitates AM placement. Our technique is easy to use and minimizes suturing and manipulation of ocular tissues, resulting in decreased operative time. This technique may be applied in the management of multiple ocular surface disease processes, including chemical or thermal burns, severe ocular graft versus host disease (GVHD), and other autoimmune diseases., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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15. Bone Mineral Density in Navajo Men and Women and Comparison to Non-Hispanic Whites from NHANES (2005-2008).
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Miller K, Frech T, Greene T, Ma KN, McFadden M, Tom-Orme L, Slattery ML, and Murtaugh MA
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- Absorptiometry, Photon, Cross-Sectional Studies, Female, Humans, Male, White People, Bone Density, Indians, North American, Nutrition Surveys, Osteoporosis ethnology
- Abstract
Purpose: To describe bone mineral density (BMD) at the hip and spine and prevalence of low bone mass and osteoporosis in Navajo men and women across age, gender, and body mass index (BMI) compared with non-Hispanic (NH) Whites from NHANES (2005-2008)., Methods: Cross-sectional dual energy x-ray absorptiometry measurements at the hip and spine in 1,097 participants from the Education and Research Towards Health study., Results: Bone mineral density was lower among younger Navajo than NH-Whites at lower BMI, and in overweight, younger men at lumbar spine and total hip. Spine BMD was lower in Navajo women, across BMI. Prevalence of low bone mass and osteoporosis in Navajo was higher than NH-Whites, particularly among women., Conclusions: Further research is needed to understand if lower BMD among younger Navajo signals a risk for future fracture, and fracture risk relative to BMD, given the challenges in health care access and fracture morbidity among minorities.
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- 2016
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16. Ferric Citrate Reduces Intravenous Iron and Erythropoiesis-Stimulating Agent Use in ESRD.
- Author
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Umanath K, Jalal DI, Greco BA, Umeukeje EM, Reisin E, Manley J, Zeig S, Negoi DG, Hiremath AN, Blumenthal SS, Sika M, Niecestro R, Koury MJ, Ma KN, Greene T, Lewis JB, and Dwyer JP
- Subjects
- Administration, Intravenous, Anemia etiology, Drug Therapy, Combination, Female, Humans, Kidney Failure, Chronic complications, Male, Middle Aged, Anemia drug therapy, Ferric Compounds therapeutic use, Hematinics administration & dosage, Iron administration & dosage
- Abstract
Ferric citrate (FC) is a phosphate binder with shown efficacy and additional effects on iron stores and use of intravenous (iv) iron and erythropoiesis-stimulating agents (ESAs). We provide detailed analyses of changes in iron/hematologic parameters and iv iron/ESA use at time points throughout the active control period of a phase 3 international randomized clinical trial. In all, 441 subjects were randomized (292 to FC and 149 to sevelamer carbonate and/or calcium acetate [active control (AC)]) and followed for 52 weeks. Subjects on FC had increased ferritin and transferrin saturation (TSAT) levels compared with subjects on AC by week 12 (change in ferritin, 114.1±29.35 ng/ml; P<0.001; change in TSAT, 8.62%±1.57%; P<0.001). Change in TSAT plateaued at this point, whereas change in ferritin increased through week 24, remaining relatively stable thereafter. Subjects on FC needed less iv iron compared with subjects on AC over 52 weeks (median [interquartile range] dose=12.9 [1.0-28.9] versus 26.8 [13.4-47.6] mg/wk; P<0.001), and the percentage of subjects not requiring iv iron was higher with FC (P<0.001). Cumulative ESA over 52 weeks was lower with FC than AC (median [interquartile range] dose=5303 [2023-9695] versus 6954 [2664-12,375] units/wk; P=0.04). Overall, 90.3% of subjects on FC and 89.3% of subjects on AC experienced adverse events. In conclusion, treatment with FC as a phosphate binder results in increased iron parameters apparent after 12 weeks and reduces iv iron and ESA use while maintaining hemoglobin over 52 weeks, with a safety profile similar to that of available binders., (Copyright © 2015 by the American Society of Nephrology.)
- Published
- 2015
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17. Response.
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Ng K, Chan AT, and Fuchs CS
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- Female, Humans, Male, Anti-Inflammatory Agents, Non-Steroidal administration & dosage, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Aspirin administration & dosage, Colonic Neoplasms pathology, Colonic Neoplasms prevention & control, Cyclooxygenase 2 Inhibitors administration & dosage, Neoplasm Recurrence, Local prevention & control
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- 2015
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18. Identification of differentially expressed proteins and validation of the changes of N-ethylmaleimide-sensitive factor in rats with focal cerebral ischemia after transection of the cervical sympathetic trunk.
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Qu Y, Ma KN, and Li XZ
- Subjects
- Animals, Brain Injuries genetics, Brain Injuries pathology, Brain Ischemia genetics, Brain Ischemia pathology, Hippocampus metabolism, Hippocampus pathology, Male, N-Ethylmaleimide-Sensitive Proteins genetics, Rats, Rats, Wistar, Stellate Ganglion pathology, Brain Injuries metabolism, Brain Ischemia metabolism, Gene Expression Regulation, N-Ethylmaleimide-Sensitive Proteins biosynthesis, Stellate Ganglion metabolism, Transfection
- Abstract
Stellate ganglion blockade (SGB) protects patients from focal cerebral ischemic injury, and transection of the cervical sympathetic trunk (TCST) in a rat model can mimic SGB in humans. The purpose of this study was to investigate the mechanisms underlying the neuroprotective effects of TCST on neuronal damage in the hippocampus in a rat model of middle cerebral artery occlusion (MCAO) in an attempt to elucidate the neuroprotective effects of SGB. The modified method of Zea Longa was used to establish the permanent MCAO model. Male Wistar rats were randomly divided into three groups: sham-operated group, MCAO group, and TCST group. The animals in TCST group were sacrificed 48 h after TCST which was performed after the establishment of the MCAO model. Proteins were extracted from the ipsilateral hippocampus and analyzed by two-dimensional difference gel electrophoresis (2D-DIGE) and peptide mass fingerprinting (PMF). The levels of N-ethylmaleimide-sensitive factor (NSF) were measured as well. The results showed that 11 types of proteins were identified by 2D-DIGE. The expressions of eight proteins were changed both in the sham-operated and TCST groups, and the expressions of the other three proteins were changed in all three groups. Moreover, the expression of NSF was higher in the TCST group than in the MCAO group but lower in the MCAO group than in sham-operated group. The ratio of NSF expression between the MCAO group and shamoperated group was -1.37 (P<0.05), whereas that between the TCST group and MCAO group was 1.35 (P<0.05). Our results imply that TCST increases the expression of NSF in the hippocampus of adult rats with focal cerebral ischemia, which may contribute to the protection of the injured brain. Our study provides a theoretical basis for the therapeutic application of SGB to patients with permanent cerebral ischemia.
- Published
- 2014
- Full Text
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19. Aspirin and COX-2 inhibitor use in patients with stage III colon cancer.
- Author
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Ng K, Meyerhardt JA, Chan AT, Sato K, Chan JA, Niedzwiecki D, Saltz LB, Mayer RJ, Benson AB 3rd, Schaefer PL, Whittom R, Hantel A, Goldberg RM, Venook AP, Ogino S, Giovannucci EL, and Fuchs CS
- Subjects
- Adult, Age Factors, Aged, Camptothecin administration & dosage, Camptothecin analogs & derivatives, Chemotherapy, Adjuvant, Colonic Neoplasms drug therapy, Colonic Neoplasms mortality, Disease-Free Survival, Female, Fluorouracil administration & dosage, Humans, Irinotecan, Leucovorin administration & dosage, Male, Middle Aged, Neoplasm Recurrence, Local mortality, Neoplasm Staging, Odds Ratio, Prospective Studies, Sex Factors, Treatment Outcome, Anti-Inflammatory Agents, Non-Steroidal administration & dosage, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Aspirin administration & dosage, Colonic Neoplasms pathology, Colonic Neoplasms prevention & control, Cyclooxygenase 2 Inhibitors administration & dosage, Neoplasm Recurrence, Local prevention & control
- Abstract
We conducted a prospective, observational study of aspirin and COX-2 inhibitor use and survival in stage III colon cancer patients enrolled in an adjuvant chemotherapy trial. Among 799 eligible patients, aspirin use was associated with improved recurrence-free survival (RFS) (multivariable hazard ratio [HR] = 0.51, 95% confidence interval [CI] = 0.28 to 0.95), disease-free survival (DFS) (HR = 0.68, 95% CI = 0.42 to 1.11), and overall survival (OS) (HR = 0.63, 95% CI = 0.35 to 1.12). Adjusted HRs for DFS and OS censored at five years (in an attempt to minimize misclassification from noncancer death) were 0.61 (95% CI = 0.36 to 1.04) and 0.48 (95% CI = 0.23 to 0.99). Among 843 eligible patients, those who used COX-2 inhibitors had multivariable HRs for RFS, DFS, and OS of 0.53 (95% CI = 0.27 to 1.04), 0.60 (95% CI = 0.33 to 1.08), and 0.50 (95% CI = 0.23 to 1.07), and HRs of 0.47 (95% CI = 0.24 to 0.91) and 0.26 (95% CI = 0.08 to 0.81) for DFS and OS censored at five years. Aspirin and COX-2 inhibitor use may be associated with improved outcomes in stage III colon cancer patients., (© The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2014
- Full Text
- View/download PDF
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